pycom09g15450

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011362
Physical Location & Seq
Reverse (-)
15316633 .. 15318404
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1554 bp
ATGTTTTCATCCCTGCCCCGCAGGGAAAAAACAACATCCTCCGCCCACCTAAAAAGCTCACAAAACCAACTCAAGCATGGCCCTTCTAACCTGGAACGTGCTTCTCTTGATGTTCAAATACCACTTGCCCTAGTTCTACGTGAAGAAGATAACAACACAGATAACTTTAGGCTTTTAGAAAGCATTGAAGCTTTTCTGTGCGGGTGGGATTCTGGGAACTCTGCCTCTCAGGTTTTGGATTTGATGAACCTAAAGAACAAAGTTCATAATGAGAAGCCACTTGAGTTCGAGAATGAGCTTCTTAGTGGGTCTTTTGTTTCTACAGAAAGTTTTCGTGGAACACCATACATGGAAGCTATACTAGACAGGAGAACGAAAGATGGAGGCGTACTGGTGAAGAAGTGGCTTCAAGAAGCTCTTCGTCGGGAAAATATAACTGTGAATGTCAAATCGCGTCCTGGTTTTGTTACAAAGTCAGAGTTGCAACTTATGGTAAAAGCCCTAGCTAAAACGCAGTCATCTTTACTGAGAAATAAGGGAATTATTCAATTAGCAGCAGCTGCTCTGGTTGCCCTTGACGAATCTAATTCGGCCAGATGGGAAGCATTTATGAGTGCAGAGAGAATATTGAGTGTTGTAAGTGCTGGAGACACAACCCAGAGTCTTTCTGCTCAAATTGATGATCTTATCAATAAGACGGCTCTGATGGGATTGCATGGACAGAAGAACAACAAGTCGGAGGCCTCAAAAGGACTACTTTCTTTTCAGGATGCTTTGCTCCTTATGATTTCTGGATATGTATTAGCAGGTGAGAATTTTCCGACATCTGGGTCTGATGGCCCTTTTTCTTGGCAAGAGGAGAAATTGTTGAAGGATTCTATTGTTGAAGCAATTCTTGAAAACCCATCAATAGCAAAACTCAAGTTTCTTCATGGTCTAATGGAAGAGCTCGAGACCAACTTAAGCAAAGAAACGTCTTCAGATCAAATAGACATCGATGATTTTGATGACGATCAGTGGGGTAAATGGGGTGATGAAGAAGTTGATAATAAAAATAACAGTAAAGAGCAAGTGTATAGTGACATGCAGCTGAAGTTGGAGCTGCGTGATAGGGTGGATAATCTTTTCAAATTCCTTCACAAGCTATCTAGTTTGAAGAGTAGGAACATACCATTGAAGGACGGGTCATTGTCTTCAGAAAATAATTTCAGCGGAGATCCATATGCAAGCAGAGGACTGCTCTATAAGCTTCTAACAAGGGTTTTGGCGAAGAACGACGTACCTGGTCTGGAGTATCACTCGTCTACTGTGGGACAACTTTTTAAAAGTGGTTTCAGAAGATTTGGCCTTGGGCAGGCGAAACCAAGTTTAGCCGACCAAAATATCATCCTGGTTTTTGTCATTGGAGGAATCAATAGTGTCGAGGTTCGTGAAGCTCAAGAGGCATTATCCGAGAGTGGAAGGCCGGATATTGAATTGGTTCTTGGAGGAACAACTCTCCTTACTCCTGACGACATGCTCGACTTACTACTGGGCAAGTCTAGTTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006508 GO:0006624 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016485 GO:0019538 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

518

Amino Acids

57.34

Weight (kDa)

5.21

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 202 - 508 1.4e-08 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42700 AT2G42700 AT2G42700
fragaria_vesca FvH4_6g27600 FvH4_6g27600
malus_domestica MD11G1004500.v1.1
prunus_persica Prupe.3G115700_v2.0.a1
pyrus_communis pycom09g15450
rosa_chinensis RchiOBHm_Chr2g0130091
rosa_laevigata RLG00000019126
rosa_multiflora Rmu_co7958197.1_g000001 Rmu_co8394299.1_g000001 Rmu_sc0010460.1_g000011
rosa_roxburghii Rroxscaffold_2G00114090
rosa_rugosa Rorug02G0291000
rosa_samantha Rh2AG342800 Rh2BG350500 Rh2CG329900 Rh2DG369000
rosa_wichuraiana Rw2G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 797
AasI GACNNNNNNGTC 1 cut(s) 829
Acc36I ACCTGC 1 cut(s) 797
AccI GTMKAC 1 cut(s) 1304
AccII CGCG 1 cut(s) 454
AciI CCGC 4 cut(s) 19, 42, 201, 1212
AclWI GGATC 1 cut(s) 1211
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 2 cut(s) 814, 1130
AcuI CTGAAG 3 cut(s) 963, 1112, 1179
AfaI GTAC 2 cut(s) 390, 1281
AfiI CCNNNNNNNGG 2 cut(s) 827, 1354
AflII CTTAAG 1 cut(s) 961
AjnI CCWGG 4 cut(s) 90, 457, 1282, 1389
AjuI GAANNNNNNNTTGG 2 cut(s) 1467, 1499
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 642, 947
AlwI GGATC 1 cut(s) 1211
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 950
AoxI GGCC 6 cut(s) 79, 591, 741, 838, 1345, 1463
ApeKI GCWGC 5 cut(s) 554, 557, 560, 1087, 1102
ApoI RAATTY 2 cut(s) 814, 1130
Asp700I GAANNNNTTC 5 cut(s) 192, 330, 417, 891, 1479
AspS9I GGNCC 2 cut(s) 80, 839
AsuHPI GGTGA 3 cut(s) 406, 821, 1043
AvaI CYCGRG 1 cut(s) 950
BanII GRGCYC 1 cut(s) 951
BbsI GAAGAC 2 cut(s) 969, 1185
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 5 cut(s) 547, 566, 569, 1089, 1099
BccI CCATC 5 cut(s) 374, 591, 700, 830, 913
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 4 cut(s) 92, 459, 1284, 1391
BcoDI GTCTC 2 cut(s) 642, 947
BfaI CTAG 5 cut(s) 131, 362, 503, 1149, 1542
BfmI CTRYAG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 961
BfuAI ACCTGC 1 cut(s) 797
BisI GCNGC 5 cut(s) 555, 558, 561, 1088, 1103
BlsI GCNGC 5 cut(s) 556, 559, 562, 1089, 1104
Bme1390I CCNGG 4 cut(s) 92, 459, 1284, 1391
BmeT110I CYCGRG 1 cut(s) 950
BmgT120I GGNCC 2 cut(s) 80, 839
BmrFI CCNGG 4 cut(s) 92, 459, 1284, 1391
BmrI ACTGGG 1 cut(s) 1541
BmsI GCATC 1 cut(s) 760
BmuI ACTGGG 1 cut(s) 1541
BpiI GAAGAC 2 cut(s) 969, 1185
BplI GAGNNNNNCTC 4 cut(s) 1224, 1256, 1283, 1315
BpmI CTGGAG 2 cut(s) 666, 1310
BpuEI CTTGAG 4 cut(s) 56, 302, 905, 1422
Bsa29I ATCGAT 1 cut(s) 996
BsaAI YACGTR 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 1 cut(s) 1348
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 827, 1354
Bse1I ACTGG 2 cut(s) 396, 1536
Bse8I GATNNNNATC 1 cut(s) 1011
BseBI CCWGG 4 cut(s) 92, 459, 1284, 1391
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 1 cut(s) 1348
BseGI GGATG 4 cut(s) 8, 35, 775, 1386
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 2 cut(s) 827, 1354
BseMII CTCAG 2 cut(s) 242, 518
BseNI ACTGG 2 cut(s) 396, 1536
BseRI GAGGAG 1 cut(s) 872
BseXI GCAGC 5 cut(s) 547, 566, 569, 1089, 1099
BsgI GTGCAG 1 cut(s) 636
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 6 cut(s) 81, 593, 743, 840, 1347, 1465
BshVI ATCGAT 1 cut(s) 996
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 950
BsiSI CCGG 1 cut(s) 1466
BslFI GGGAC 1 cut(s) 1326
BslI CCNNNNNNNGG 2 cut(s) 827, 1354
BsmAI GTCTC 2 cut(s) 642, 947
BsmFI GGGAC 1 cut(s) 1326
BsnI GGCC 6 cut(s) 81, 593, 743, 840, 1347, 1465
Bso31I GGTCTC 1 cut(s) 947
BsoBI CYCGRG 1 cut(s) 950
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 4 cut(s) 682, 982, 1012, 1216
BspACI CCGC 4 cut(s) 19, 42, 201, 1212
BspANI GGCC 6 cut(s) 81, 593, 743, 840, 1347, 1465
BspCNI CTCAG 2 cut(s) 241, 519
BspDI ATCGAT 1 cut(s) 996
BspFNI CGCG 1 cut(s) 454
BspMI ACCTGC 1 cut(s) 797
BspPI GGATC 1 cut(s) 1211
BspQI GCTCTTC 2 cut(s) 423, 939
BspTI CTTAAG 1 cut(s) 961
BspTNI GGTCTC 1 cut(s) 947
BsrI ACTGG 2 cut(s) 396, 1536
BssECI CCNNGG 1 cut(s) 1348
BssMI GATC 4 cut(s) 682, 982, 1012, 1216
BssT1I CCWWGG 1 cut(s) 1348
Bst2UI CCWGG 4 cut(s) 92, 459, 1284, 1391
Bst4CI ACNGT 3 cut(s) 439, 1061, 1309
Bst6I CTCTTC 3 cut(s) 423, 939, 1151
BstAFI CTTAAG 1 cut(s) 961
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 1228, 1356
BstDEI CTNAG 3 cut(s) 228, 302, 527
BstENI CCTNNNNNAGG 1 cut(s) 1352
BstF5I GGATG 4 cut(s) 8, 35, 775, 1386
BstFNI CGCG 1 cut(s) 454
BstKTI GATC 4 cut(s) 685, 985, 1015, 1219
BstMAI GTCTC 2 cut(s) 642, 947
BstMBI GATC 4 cut(s) 682, 982, 1012, 1216
BstMWI GCNNNNNNNGC 4 cut(s) 560, 569, 1246, 1442
BstNI CCWGG 4 cut(s) 92, 459, 1284, 1391
BstNSI RCATGY 2 cut(s) 1087, 1519
BstSCI CCNGG 4 cut(s) 90, 457, 1282, 1389
BstSFI CTRYAG 1 cut(s) 321
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 5 cut(s) 547, 566, 569, 1089, 1099
BstV2I GAAGAC 2 cut(s) 969, 1185
BstX2I RGATCY 1 cut(s) 1216
BstYI RGATCY 1 cut(s) 1216
Bsu15I ATCGAT 1 cut(s) 996
BsuRI GGCC 6 cut(s) 81, 593, 743, 840, 1347, 1465
BsuTUI ATCGAT 1 cut(s) 996
BtsCI GGATG 4 cut(s) 8, 35, 775, 1386
BtsIMutI CAGTG 1 cut(s) 1022
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 2 cut(s) 1228, 1356
CaiI CAGNNNCTG 1 cut(s) 560
Cfr13I GGNCC 2 cut(s) 80, 839
ClaI ATCGAT 1 cut(s) 996
CseI GACGC 1 cut(s) 443
CsiI ACCWGGT 1 cut(s) 1282
Csp6I GTAC 2 cut(s) 389, 1280
CviAII CATG 6 cut(s) 77, 349, 716, 932, 1084, 1516
CviQI GTAC 2 cut(s) 389, 1280
DdeI CTNAG 3 cut(s) 228, 302, 527
DpnI GATC 4 cut(s) 684, 984, 1014, 1218
DpnII GATC 4 cut(s) 682, 982, 1012, 1216
DraI TTTAAA 1 cut(s) 1324
DrdI GACNNNNNNGTC 1 cut(s) 829
DseDI GACNNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 3 cut(s) 423, 939, 1151
EarI CTCTTC 3 cut(s) 423, 939, 1151
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 1 cut(s) 1348
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 951
Eco31I GGTCTC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 3 cut(s) 963, 1112, 1179
Eco88I CYCGRG 1 cut(s) 950
EcoICRI GAGCTC 1 cut(s) 949
EcoNI CCTNNNNNAGG 1 cut(s) 1352
EcoRII CCWGG 4 cut(s) 90, 457, 1282, 1389
EcoT14I CCWWGG 1 cut(s) 1348
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 1348
FaeI CATG 6 cut(s) 80, 352, 719, 935, 1087, 1519
FalI AAGNNNNNCTT 6 cut(s) 402, 434, 741, 773, 879, 911
FaqI GGGAC 1 cut(s) 1326
FatI CATG 6 cut(s) 76, 348, 715, 931, 1083, 1515
FauI CCCGC 2 cut(s) 26, 194
FauNDI CATATG 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 1304
Fnu4HI GCNGC 5 cut(s) 555, 558, 561, 1088, 1103
FokI GGATG 3 cut(s) 22, 782, 1373
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 5 cut(s) 555, 558, 561, 1088, 1103
FspBI CTAG 5 cut(s) 131, 362, 503, 1149, 1542
GluI GCNGC 5 cut(s) 555, 558, 561, 1088, 1103
GsuI CTGGAG 2 cut(s) 666, 1310
HaeIII GGCC 6 cut(s) 81, 593, 743, 840, 1347, 1465
HapII CCGG 1 cut(s) 1466
HgaI GACGC 1 cut(s) 443
Hin1II CATG 6 cut(s) 80, 352, 719, 935, 1087, 1519
HindIII AAGCTT 2 cut(s) 189, 1247
HinfI GANTC 5 cut(s) 209, 581, 661, 875, 1410
HpaII CCGG 1 cut(s) 1466
HphI GGTGA 3 cut(s) 406, 821, 1043
Hpy166II GTNNAC 1 cut(s) 1305
Hpy8I GTNNAC 1 cut(s) 1305
Hpy99I CGWCG 2 cut(s) 426, 1280
HpyAV CCTTC 5 cut(s) 93, 865, 1145, 1171, 1455
HpyCH4III ACNGT 3 cut(s) 439, 1061, 1309
HpyCH4IV ACGT 4 cut(s) 97, 139, 974, 1278
HpyCH4V TGCA 5 cut(s) 484, 617, 715, 1087, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 560, 569, 1246, 1442
HpyF3I CTNAG 3 cut(s) 228, 302, 527
HpySE526I ACGT 4 cut(s) 97, 139, 974, 1278
Hsp92II CATG 6 cut(s) 80, 352, 719, 935, 1087, 1519
Kzo9I GATC 4 cut(s) 682, 982, 1012, 1216
LguI GCTCTTC 2 cut(s) 423, 939
LmnI GCTCC 2 cut(s) 783, 1099
Lsp1109I GCAGC 5 cut(s) 547, 566, 569, 1089, 1099
LweI GCATC 1 cut(s) 760
MabI ACCWGGT 1 cut(s) 1282
MaeI CTAG 5 cut(s) 131, 362, 503, 1149, 1542
MaeII ACGT 4 cut(s) 97, 139, 974, 1278
MaeIII GTNAC 3 cut(s) 466, 1079, 1544
MalI GATC 4 cut(s) 684, 984, 1014, 1218
MboI GATC 4 cut(s) 682, 982, 1012, 1216
MflI RGATCY 1 cut(s) 1216
MhlI GDGCHC 1 cut(s) 951
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 3 cut(s) 717, 845, 1077
MroXI GAANNNNTTC 5 cut(s) 192, 330, 417, 891, 1479
MseI TTAA 2 cut(s) 962, 1323
MspA1I CMGCKG 3 cut(s) 560, 1090, 1212
MspCI CTTAAG 1 cut(s) 961
MspI CCGG 1 cut(s) 1466
MspR9I CCNGG 4 cut(s) 92, 459, 1284, 1391
MvaI CCWGG 4 cut(s) 92, 459, 1284, 1391
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 4 cut(s) 560, 569, 1246, 1442
NdeI CATATG 1 cut(s) 1222
NdeII GATC 4 cut(s) 682, 982, 1012, 1216
NlaIII CATG 6 cut(s) 80, 352, 719, 935, 1087, 1519
NmuCI GTSAC 1 cut(s) 1079
NspI RCATGY 2 cut(s) 1087, 1519
PaeR7I CTCGAG 1 cut(s) 950
PaqCI CACCTGC 1 cut(s) 797
PceI AGGCCT 1 cut(s) 743
PciSI GCTCTTC 2 cut(s) 423, 939
PcsI WCGNNNNNNNCGW 1 cut(s) 1518
PdmI GAANNNNTTC 5 cut(s) 192, 330, 417, 891, 1479
PfeI GAWTC 4 cut(s) 209, 581, 875, 1410
PkrI GCNGC 5 cut(s) 556, 559, 562, 1089, 1104
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
Ppu21I YACGTR 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 951
Psp6I CCWGG 4 cut(s) 90, 457, 1282, 1389
PspGI CCWGG 4 cut(s) 90, 457, 1282, 1389
PspPI GGNCC 2 cut(s) 80, 839
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 1 cut(s) 1216
PvuII CAGCTG 2 cut(s) 560, 1090
RsaI GTAC 2 cut(s) 390, 1281
RsaNI GTAC 2 cut(s) 389, 1280
SacI GAGCTC 1 cut(s) 951
SapI GCTCTTC 2 cut(s) 423, 939
SaqAI TTAA 2 cut(s) 962, 1323
SatI GCNGC 5 cut(s) 555, 558, 561, 1088, 1103
Sau3AI GATC 4 cut(s) 682, 982, 1012, 1216
Sau96I GGNCC 2 cut(s) 80, 839
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 4 cut(s) 92, 459, 1284, 1391
SduI GDGCHC 1 cut(s) 951
SexAI ACCWGGT 1 cut(s) 1282
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 1 cut(s) 321
Sfr274I CTCGAG 1 cut(s) 950
SlaI CTCGAG 1 cut(s) 950
SmlI CTYRAG 6 cut(s) 71, 281, 920, 950, 961, 1437
SmoI CTYRAG 6 cut(s) 71, 281, 920, 950, 961, 1437
SseBI AGGCCT 1 cut(s) 743
SsiI CCGC 4 cut(s) 19, 42, 201, 1212
SspI AATATT 1 cut(s) 627
SspMI CTAG 5 cut(s) 131, 362, 503, 1149, 1542
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 4 cut(s) 90, 457, 1282, 1389
StyI CCWWGG 1 cut(s) 1348
TaaI ACNGT 3 cut(s) 439, 1061, 1309
TaiI ACGT 4 cut(s) 100, 142, 977, 1281
TaqI TCGA 5 cut(s) 288, 951, 996, 1422, 1521
TfiI GAWTC 4 cut(s) 209, 581, 875, 1410
Tru1I TTAA 2 cut(s) 962, 1323
Tru9I TTAA 2 cut(s) 962, 1323
TscAI CASTG 1 cut(s) 1022
TseFI GTSAC 1 cut(s) 1079
TseI GCWGC 5 cut(s) 554, 557, 560, 1087, 1102
Tsp45I GTSAC 1 cut(s) 1079
TspDTI ATGAA 4 cut(s) 254, 260, 920, 1050
TspRI CASTG 1 cut(s) 1022
Vha464I CTTAAG 1 cut(s) 961
XagI CCTNNNNNAGG 1 cut(s) 1352
XapI RAATTY 2 cut(s) 814, 1130
XceI RCATGY 2 cut(s) 1087, 1519
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 1304
XmnI GAANNNNTTC 5 cut(s) 192, 330, 417, 891, 1479
XspI CTAG 5 cut(s) 131, 362, 503, 1149, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.