pycom09g19420

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012149
Physical Location & Seq
Reverse (-)
20274809 .. 20278054
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19420.4

Sequence Viewer

Length: 1470 bp
ATGAGATGGATATTGAAAAGATTGAAACATCCCAAAGGTCACGCGGTAACCGGTTTCGCCAAATCACCCGACCATATATATACCGGTTCAGCCACAACTCCCCCGATTTTTGTTGATTCAGCGAAAGAAAAGGAAAAACGAACCGAAAGTGCGAAATTTTTTTCCAGAAACCGCGGGAAAAAAAAAAAAAAAAAAGTCATCGGATTCTCTTCCTCCTCGTCTCCGTCGTCTCCGTCGTTGGTTGTCGAAGTTTTGTTCGAGTTTAACAACAACATGGTGGCAGAAGCTAAGGTTGTGTGTCAGCAGAGCGTGCCGGTGCTGGATGTTCAGTACTTTACCAAAAATGTTCACGAAGTTGAAGATGTAGTTGCAGTTACCCAATCGATTTCGTCGCCCAAGAGTTTTGACCGGAATCTTTCTTCGGAACCGGTCTCCGCCGCCGCTCTCTTTAGCTCGCAAGCCGTCGATGCTAAGCCTGTGGACAAAATTCCAGATTTGGTTCCTGCCTCGGCCGACCAGCAATTCTTCCCCAAGCTGCGTTCTGGTAGCTTTGCTGACATTGGACCCCGTAGATACATGGAAGATGAACATATTCTAATAGATGATCTATCTTCACACTTGGGACCACTCTTTAGTTTTCCTAAGCCAAATGCATTTTATGGGGTATTTGATGGACATGGAGGACCTGAAGCAGCTGCTTATGTCCGGAAAAATGTGCTCAGACTCTTTTTTGAAGATGCCAATTTTCCCCGAACTTCTGACGTTGATGAAATTTTCTCAGAGAAGCTTGAGAACTCCCTCCGCAAGGCATTTCATGTGGTTGACCTTGCTTTGGCTGATGACTGCAGTGTAAGCAGTTCTTCGGGGACAACAGCATTAACTGCTATGATATTTGGAAGGCTTTTAATGGTGGCCAATGCTGGGGACTGCCGAGCAGTACTCTGTCGGAAAGGAGAAGCCATTGATATGTCTCAAGACCACAGGCCAATATATCCCTCAGAAAGGAGGCGGATAGAAGAATTAGGTGGATATGTTGATGATGGGTATCTGAACGGTGTCTTATCAGTTACCCGAGCATTAGGCGACTGGGACATGAAGTTTCCTCGTGGATCTTCTTCACCCCTAATCGCCGAGCCCGAGTTCCGGCAGGTGGTTTTAACAGAGGATGACGAGTTTCTAATTATAGGGTGTGATGGGATTTGGGATGTGATGTCTAGTCAGCATGCTGTTAGCCTTGTTCGCCGCGGGCTGAGGCGTCATGACGACCCCGAGCAGTGTGCCAGGGACCTTGTCATGGAGGCTCTTCACCTCAACACGTTTGATAACCTCACGGTGATCATTGTATGCTTCTCTCCCCTTGAGCACCGGGATCTATCGCCACAGCGAGAACGGAGATTAAAGTGCTGCAGCCTCTCTGCTGAGGCCCTTTGTAGCTTGAGGGCCTTGTTGGACAGTAGTGCCAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

490

Amino Acids

54.18

Weight (kDa)

6.15

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62260 AT3G62260 AT3G62260
fragaria_vesca FvH4_1g26700 FvH4_1g26700
malus_domestica MD09G1287100.v1.1
prunus_persica Prupe.3G006100_v2.0.a1
pyrus_communis pycom09g19420 pycom17g27730
rosa_chinensis RchiOBHm_Chr3g0486881
rosa_laevigata RLG00000023049
rosa_multiflora Rmu_sc0003470.1_g000010
rosa_roxburghii Rroxscaffold_6G00395430
rosa_rugosa Rorug03G0228500
rosa_samantha Rh3AG277700 Rh3BG313500 Rh3CG312000 Rh3DG308300
rosa_wichuraiana Rw3G024660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1138
Acc36I ACCTGC 1 cut(s) 1138
AccBSI CCGCTC 1 cut(s) 443
AccII CGCG 3 cut(s) 44, 174, 1245
AccIII TCCGGA 1 cut(s) 705
AclWI GGATC 2 cut(s) 1117, 1377
AcoI YGGCCR 2 cut(s) 510, 912
AcsI RAATTY 3 cut(s) 155, 486, 771
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 1255
AfaI GTAC 2 cut(s) 332, 939
AfiI CCNNNNNNNGG 7 cut(s) 805, 832, 921, 1002, 1143, 1150, 1294
AflIII ACRYGT 1 cut(s) 1314
AgeI ACCGGT 3 cut(s) 50, 83, 427
AgsI TTSAA 4 cut(s) 16, 25, 359, 734
AjnI CCWGG 1 cut(s) 1280
AluBI AGCT 8 cut(s) 287, 453, 535, 549, 695, 787, 1434, 1467
AluI AGCT 8 cut(s) 287, 453, 535, 549, 695, 787, 1434, 1467
Alw21I GWGCWC 2 cut(s) 720, 1365
Alw26I GTCTC 4 cut(s) 225, 234, 436, 975
AlwI GGATC 2 cut(s) 1117, 1377
AlwNI CAGNNNCTG 1 cut(s) 1467
Ama87I CYCGRG 3 cut(s) 1071, 1136, 1268
Aor13HI TCCGGA 1 cut(s) 705
AoxI GGCC 5 cut(s) 510, 912, 983, 1422, 1440
ApeKI GCWGC 5 cut(s) 535, 692, 695, 1404, 1407
ApoI RAATTY 3 cut(s) 155, 486, 771
AsiGI ACCGGT 3 cut(s) 50, 83, 427
Asp700I GAANNNNTTC 1 cut(s) 591
AspS9I GGNCC 6 cut(s) 563, 623, 683, 1285, 1423, 1440
AsuC2I CCSGG 1 cut(s) 1367
AsuHPI GGTGA 4 cut(s) 57, 1110, 1298, 1345
AvaI CYCGRG 3 cut(s) 1071, 1136, 1268
AvaII GGWCC 4 cut(s) 563, 623, 683, 1285
BalI TGGCCA 1 cut(s) 914
BanII GRGCYC 1 cut(s) 1137
BauI CACGAG 1 cut(s) 1104
Bbv12I GWGCWC 2 cut(s) 720, 1365
BbvCI CCTCAGC 2 cut(s) 1250, 1419
BbvI GCAGC 5 cut(s) 522, 682, 704, 1391, 1419
BccI CCATC 3 cut(s) 665, 1034, 1187
BceAI ACGGC 1 cut(s) 446
BcgI CGANNNNNNTGC 2 cut(s) 1259, 1293
BciT130I CCWGG 1 cut(s) 1282
BclI TGATCA 1 cut(s) 1335
BcnI CCSGG 1 cut(s) 1367
BcoDI GTCTC 4 cut(s) 225, 234, 436, 975
BfaI CTAG 1 cut(s) 1215
BfmI CTRYAG 2 cut(s) 844, 1405
BfuAI ACCTGC 1 cut(s) 1138
BisI GCNGC 8 cut(s) 438, 441, 536, 693, 696, 1243, 1405, 1408
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 8 cut(s) 439, 442, 537, 694, 697, 1244, 1406, 1409
BmcAI AGTACT 2 cut(s) 332, 939
Bme1390I CCNGG 2 cut(s) 1282, 1367
Bme18I GGWCC 4 cut(s) 563, 623, 683, 1285
BmeT110I CYCGRG 3 cut(s) 1071, 1136, 1268
BmgT120I GGNCC 6 cut(s) 563, 623, 683, 1285, 1423, 1440
BmiI GGNNCC 5 cut(s) 426, 501, 565, 624, 1286
BmrFI CCNGG 2 cut(s) 1282, 1367
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 2 cut(s) 457, 727
BmuI ACTGGG 1 cut(s) 1096
BplI GAGNNNNNCTC 2 cut(s) 924, 956
Bpu10I CCTNAGC 4 cut(s) 288, 642, 1250, 1419
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 4 cut(s) 809, 957, 1379, 1456
BpuMI CCSGG 1 cut(s) 1367
Bsa29I ATCGAT 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 1255
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 4 cut(s) 172, 507, 1243, 1281
BsaWI WCCGGW 5 cut(s) 50, 83, 408, 427, 705
Bsc4I CCNNNNNNNGG 7 cut(s) 805, 832, 921, 1002, 1143, 1150, 1294
Bse118I RCCGGY 4 cut(s) 50, 83, 313, 427
Bse1I ACTGG 2 cut(s) 1091, 1461
Bse8I GATNNNNATC 1 cut(s) 1044
BseAI TCCGGA 1 cut(s) 705
BseBI CCWGG 1 cut(s) 1282
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 4 cut(s) 172, 507, 1243, 1281
BseGI GGATG 4 cut(s) 28, 328, 1171, 1210
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 7 cut(s) 805, 832, 921, 1002, 1143, 1150, 1294
BseMII CTCAG 5 cut(s) 733, 792, 1011, 1241, 1410
BseNI ACTGG 2 cut(s) 1091, 1461
BseRI GAGGAG 1 cut(s) 205
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 5 cut(s) 522, 682, 704, 1391, 1419
BseYI CCCAGC 1 cut(s) 920
Bsh1236I CGCG 3 cut(s) 44, 174, 1245
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 5 cut(s) 512, 914, 985, 1424, 1442
BshTI ACCGGT 3 cut(s) 50, 83, 427
BshVI ATCGAT 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 720, 1365
BsiHKCI CYCGRG 3 cut(s) 1071, 1136, 1268
BsiSI CCGG 8 cut(s) 51, 84, 314, 409, 428, 706, 1144, 1366
BslFI GGGAC 5 cut(s) 636, 880, 938, 1103, 1298
BslI CCNNNNNNNGG 7 cut(s) 805, 832, 921, 1002, 1143, 1150, 1294
BsmAI GTCTC 4 cut(s) 225, 234, 436, 975
BsmBI CGTCTC 2 cut(s) 225, 234
BsmFI GGGAC 5 cut(s) 636, 880, 938, 1103, 1298
BsnI GGCC 5 cut(s) 512, 914, 985, 1424, 1442
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 3 cut(s) 1071, 1136, 1268
Bsp1286I GDGCHC 3 cut(s) 720, 1137, 1365
Bsp13I TCCGGA 1 cut(s) 705
Bsp143I GATC 4 cut(s) 604, 1109, 1335, 1369
Bsp1720I GCTNAGC 1 cut(s) 471
BspANI GGCC 5 cut(s) 512, 914, 985, 1424, 1442
BspCNI CTCAG 5 cut(s) 732, 791, 1010, 1242, 1411
BspDI ATCGAT 1 cut(s) 383
BspEI TCCGGA 1 cut(s) 705
BspFNI CGCG 3 cut(s) 44, 174, 1245
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 5 cut(s) 426, 501, 565, 624, 1286
BspMAI CTGCAG 2 cut(s) 848, 1409
BspMI ACCTGC 1 cut(s) 1138
BspPI GGATC 2 cut(s) 1117, 1377
BspQI GCTCTTC 1 cut(s) 1308
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 443
BsrFI RCCGGY 4 cut(s) 50, 83, 313, 427
BsrI ACTGG 2 cut(s) 1091, 1461
BssAI RCCGGY 4 cut(s) 50, 83, 313, 427
BssECI CCNNGG 4 cut(s) 172, 507, 1243, 1281
BssMI GATC 4 cut(s) 604, 1109, 1335, 1369
BssNI GRCGYC 1 cut(s) 1255
BssSI CACGAG 1 cut(s) 1104
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 1 cut(s) 1282
Bst4CI ACNGT 3 cut(s) 1055, 1333, 1454
Bst6I CTCTTC 2 cut(s) 214, 1308
BstACI GRCGYC 1 cut(s) 1255
BstAPI GCANNNNNTGC 2 cut(s) 310, 881
BstC8I GCNNGC 5 cut(s) 311, 455, 459, 1224, 1247
BstDEI CTNAG 8 cut(s) 288, 471, 642, 719, 778, 997, 1250, 1419
BstDSI CCRYGG 2 cut(s) 172, 1243
BstEII GGTNACC 1 cut(s) 46
BstENI CCTNNNNNAGG 2 cut(s) 803, 1000
BstF5I GGATG 4 cut(s) 28, 328, 1171, 1210
BstFNI CGCG 3 cut(s) 44, 174, 1245
BstKTI GATC 4 cut(s) 607, 1112, 1338, 1372
BstMAI GTCTC 4 cut(s) 225, 234, 436, 975
BstMBI GATC 4 cut(s) 604, 1109, 1335, 1369
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 5 cut(s) 310, 467, 852, 881, 1239
BstNI CCWGG 1 cut(s) 1282
BstNSI RCATGY 1 cut(s) 1226
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 1280, 1365
BstSFI CTRYAG 2 cut(s) 844, 1405
BstUI CGCG 3 cut(s) 44, 174, 1245
BstV1I GCAGC 5 cut(s) 522, 682, 704, 1391, 1419
BstX2I RGATCY 2 cut(s) 1109, 1369
BstYI RGATCY 2 cut(s) 1109, 1369
BstZI CGGCCG 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 383
BsuRI GGCC 5 cut(s) 512, 914, 985, 1424, 1442
BsuTUI ATCGAT 1 cut(s) 383
BtgI CCRYGG 2 cut(s) 172, 1243
BtsCI GGATG 4 cut(s) 28, 328, 1171, 1210
BtsI GCAGTG 2 cut(s) 853, 1280
BtsIMutI CAGTG 2 cut(s) 853, 1280
BveI ACCTGC 1 cut(s) 1138
Cac8I GCNNGC 5 cut(s) 311, 455, 459, 1224, 1247
CaiI CAGNNNCTG 1 cut(s) 1467
CciI TCATGA 1 cut(s) 1258
Cfr10I RCCGGY 4 cut(s) 50, 83, 313, 427
Cfr13I GGNCC 6 cut(s) 563, 623, 683, 1285, 1423, 1440
Cfr42I CCGCGG 2 cut(s) 175, 1246
ClaI ATCGAT 1 cut(s) 383
CseI GACGC 1 cut(s) 1244
Csp6I GTAC 2 cut(s) 331, 938
CspAI ACCGGT 3 cut(s) 50, 83, 427
CviAII CATG 8 cut(s) 274, 577, 677, 815, 1093, 1223, 1259, 1294
CviQI GTAC 2 cut(s) 331, 938
DdeI CTNAG 8 cut(s) 288, 471, 642, 719, 778, 997, 1250, 1419
DpnI GATC 4 cut(s) 606, 1111, 1337, 1371
DpnII GATC 4 cut(s) 604, 1109, 1335, 1369
EaeI YGGCCR 2 cut(s) 510, 912
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 214, 1308
EarI CTCTTC 2 cut(s) 214, 1308
EciI GGCGGA 2 cut(s) 424, 1024
EclXI CGGCCG 1 cut(s) 510
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 4 cut(s) 563, 623, 683, 1285
Eco52I CGGCCG 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 3 cut(s) 1071, 1136, 1268
Eco91I GGTNACC 1 cut(s) 46
EcoNI CCTNNNNNAGG 2 cut(s) 803, 1000
EcoO109I RGGNCCY 4 cut(s) 683, 1285, 1423, 1440
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 1280
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 1137
Esp3I CGTCTC 2 cut(s) 225, 234
FaeI CATG 8 cut(s) 277, 580, 680, 818, 1096, 1226, 1262, 1297
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FaqI GGGAC 5 cut(s) 636, 880, 938, 1103, 1298
FatI CATG 8 cut(s) 273, 576, 676, 814, 1092, 1222, 1258, 1293
FauI CCCGC 2 cut(s) 167, 1238
FbaI TGATCA 1 cut(s) 1335
Fnu4HI GCNGC 8 cut(s) 438, 441, 536, 693, 696, 1243, 1405, 1408
FokI GGATG 4 cut(s) 15, 335, 1178, 1217
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 8 cut(s) 438, 441, 536, 693, 696, 1243, 1405, 1408
FspBI CTAG 1 cut(s) 1215
GluI GCNGC 8 cut(s) 438, 441, 536, 693, 696, 1243, 1405, 1408
GsaI CCCAGC 1 cut(s) 924
HaeIII GGCC 5 cut(s) 512, 914, 985, 1424, 1442
HapII CCGG 8 cut(s) 51, 84, 314, 409, 428, 706, 1144, 1366
HgaI GACGC 1 cut(s) 1244
Hin1I GRCGYC 1 cut(s) 1255
Hin1II CATG 8 cut(s) 277, 580, 680, 818, 1096, 1226, 1262, 1297
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 4 cut(s) 116, 204, 412, 723
HpaII CCGG 8 cut(s) 51, 84, 314, 409, 428, 706, 1144, 1366
HphI GGTGA 4 cut(s) 57, 1110, 1298, 1345
Hpy166II GTNNAC 3 cut(s) 349, 481, 823
Hpy188I TCNGA 8 cut(s) 203, 424, 722, 760, 781, 948, 1000, 1050
Hpy188III TCNNGA 6 cut(s) 165, 350, 491, 706, 974, 1259
Hpy8I GTNNAC 3 cut(s) 349, 481, 823
Hpy99I CGWCG 4 cut(s) 229, 238, 394, 467
HpyAV CCTTC 1 cut(s) 891
HpyCH4III ACNGT 3 cut(s) 1055, 1333, 1454
HpyCH4IV ACGT 2 cut(s) 762, 1316
HpyCH4V TGCA 4 cut(s) 371, 653, 846, 1407
HpyF10VI GCNNNNNNNGC 5 cut(s) 310, 467, 852, 881, 1239
HpyF3I CTNAG 8 cut(s) 288, 471, 642, 719, 778, 997, 1250, 1419
HpySE526I ACGT 2 cut(s) 762, 1316
Hsp92I GRCGYC 1 cut(s) 1255
Hsp92II CATG 8 cut(s) 277, 580, 680, 818, 1096, 1226, 1262, 1297
Kpn2I TCCGGA 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 1335
KspI CCGCGG 2 cut(s) 175, 1246
Kzo9I GATC 4 cut(s) 604, 1109, 1335, 1369
LguI GCTCTTC 1 cut(s) 1308
Lsp1109I GCAGC 5 cut(s) 522, 682, 704, 1391, 1419
LweI GCATC 2 cut(s) 457, 727
MaeI CTAG 1 cut(s) 1215
MaeII ACGT 2 cut(s) 762, 1316
MaeIII GTNAC 4 cut(s) 38, 46, 373, 1066
MalI GATC 4 cut(s) 606, 1111, 1337, 1371
MbiI CCGCTC 1 cut(s) 443
MboI GATC 4 cut(s) 604, 1109, 1335, 1369
MflI RGATCY 2 cut(s) 1109, 1369
MhlI GDGCHC 3 cut(s) 720, 1137, 1365
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 7 cut(s) 155, 486, 521, 742, 771, 1019, 1179
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 1 cut(s) 717
MmeI TCCRAC 2 cut(s) 926, 1428
Mox20I TGGCCA 1 cut(s) 914
Mph1103I ATGCAT 1 cut(s) 655
MroI TCCGGA 1 cut(s) 705
MroXI GAANNNNTTC 1 cut(s) 591
MscI TGGCCA 1 cut(s) 914
MseI TTAA 5 cut(s) 264, 878, 905, 1157, 1397
MslI CAYNNNNRTG 1 cut(s) 965
Msp20I TGGCCA 1 cut(s) 914
MspA1I CMGCKG 3 cut(s) 174, 695, 1245
MspI CCGG 8 cut(s) 51, 84, 314, 409, 428, 706, 1144, 1366
MspR9I CCNGG 2 cut(s) 1282, 1367
MvaI CCWGG 1 cut(s) 1282
MvnI CGCG 3 cut(s) 44, 174, 1245
MwoI GCNNNNNNNGC 5 cut(s) 310, 467, 852, 881, 1239
NciI CCSGG 1 cut(s) 1367
NdeII GATC 4 cut(s) 604, 1109, 1335, 1369
NlaIII CATG 8 cut(s) 277, 580, 680, 818, 1096, 1226, 1262, 1297
NlaIV GGNNCC 5 cut(s) 426, 501, 565, 624, 1286
NmeAIII GCCGAG 3 cut(s) 488, 956, 1156
NmuCI GTSAC 1 cut(s) 38
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 1226
PaeI GCATGC 1 cut(s) 1226
PagI TCATGA 1 cut(s) 1258
PaqCI CACCTGC 1 cut(s) 1138
PciSI GCTCTTC 1 cut(s) 1308
PcsI WCGNNNNNNNCGW 3 cut(s) 224, 233, 1134
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 3 cut(s) 116, 204, 412
PflFI GACNNNGTC 1 cut(s) 1289
PinAI ACCGGT 3 cut(s) 50, 83, 427
PkrI GCNGC 8 cut(s) 439, 442, 537, 694, 697, 1244, 1406, 1409
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PpuMI RGGWCCY 2 cut(s) 683, 1285
Psp5II RGGWCCY 2 cut(s) 683, 1285
Psp6I CCWGG 1 cut(s) 1280
PspEI GGTNACC 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 1 cut(s) 1280
PspN4I GGNNCC 5 cut(s) 426, 501, 565, 624, 1286
PspPI GGNCC 6 cut(s) 563, 623, 683, 1285, 1423, 1440
PspPPI RGGWCCY 2 cut(s) 683, 1285
PstI CTGCAG 2 cut(s) 848, 1409
PstNI CAGNNNCTG 1 cut(s) 1467
PsuI RGATCY 2 cut(s) 1109, 1369
PsyI GACNNNGTC 1 cut(s) 1289
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 332, 939
RsaNI GTAC 2 cut(s) 331, 938
RseI CAYNNNNRTG 1 cut(s) 965
SacII CCGCGG 2 cut(s) 175, 1246
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 5 cut(s) 264, 878, 905, 1157, 1397
SatI GCNGC 8 cut(s) 438, 441, 536, 693, 696, 1243, 1405, 1408
Sau3AI GATC 4 cut(s) 604, 1109, 1335, 1369
Sau96I GGNCC 6 cut(s) 563, 623, 683, 1285, 1423, 1440
ScaI AGTACT 2 cut(s) 332, 939
SchI GAGTC 1 cut(s) 717
ScrFI CCNGG 2 cut(s) 1282, 1367
SduI GDGCHC 3 cut(s) 720, 1137, 1365
SfaNI GCATC 2 cut(s) 457, 727
SfcI CTRYAG 2 cut(s) 844, 1405
Sfr303I CCGCGG 2 cut(s) 175, 1246
SgrBI CCGCGG 2 cut(s) 175, 1246
SinI GGWCC 4 cut(s) 563, 623, 683, 1285
SmiMI CAYNNNNRTG 1 cut(s) 965
SmlI CTYRAG 4 cut(s) 788, 972, 1358, 1435
SmoI CTYRAG 4 cut(s) 788, 972, 1358, 1435
SphI GCATGC 1 cut(s) 1226
Sse9I AATT 7 cut(s) 155, 486, 521, 742, 771, 1019, 1179
SspMI CTAG 1 cut(s) 1215
StyD4I CCNGG 2 cut(s) 1280, 1365
TaaI ACNGT 3 cut(s) 1055, 1333, 1454
TaiI ACGT 2 cut(s) 765, 1319
TaqI TCGA 4 cut(s) 246, 258, 383, 465
TasI AATT 7 cut(s) 155, 486, 521, 742, 771, 1019, 1179
TatI WGTACW 2 cut(s) 330, 937
TauI GCSGC 3 cut(s) 440, 443, 1245
TfiI GAWTC 3 cut(s) 116, 204, 412
Tru1I TTAA 5 cut(s) 264, 878, 905, 1157, 1397
Tru9I TTAA 5 cut(s) 264, 878, 905, 1157, 1397
TscAI CASTG 2 cut(s) 853, 1280
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 5 cut(s) 535, 692, 695, 1404, 1407
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 4 cut(s) 600, 783, 803, 1109
TspGWI ACGGA 3 cut(s) 213, 222, 1405
TspRI CASTG 2 cut(s) 853, 1280
Tth111I GACNNNGTC 1 cut(s) 1289
VpaK11BI GGWCC 4 cut(s) 563, 623, 683, 1285
XagI CCTNNNNNAGG 2 cut(s) 803, 1000
XapI RAATTY 3 cut(s) 155, 486, 771
XceI RCATGY 1 cut(s) 1226
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 1 cut(s) 1215
ZrmI AGTACT 2 cut(s) 332, 939
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.