Rroxscaffold_6G00395430

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012149
Physical Location & Seq
Reverse (-)
16457462 .. 16460282
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00395430.1

Sequence Viewer

Length: 1188 bp
ATGGTGGCCGAAGCTAAGATCATGTGTCCGCAGAACGTTGAGGTGTTTGCTAAAGGGATTAACGTTCGTGAGGTTGAAGATGTAGTTGCATCGATTTCGCCAAAGAGTTTTGACCGAAGTCGCCGGCCTCTGGAATTTGTCTCCCCTGCTGCTGCTGCTGCTGCTTTAATATCGACCTCCCAACCTGATGTTAAACCAGCGGATAAAGTTTCAGACTTGGCTCCTGAACCAGCCAACCTGGAATTCTTTCCCAGGCTCCGTTCTGGTAGCTTCGCTGACATTGGACCGCGTAGATACATGGAAGATGAACATATACTGATAGACGATCTATCTTCCCACCTGGGATCGCTCTTTAGTTTTCCCACCCCAAGTGCTTTTTATGGGGTATTTGACGGACATGGAGGACCTGAAGCAGCAGCTTATGTTCGAAAAAATGTGATGAGACTCTTGTTTGATGATGTCAATTTTCCCCGAGCTAGTGAAGTTAATAAAATTTTCTCAGAGGAGCTCGAGAATACTCTCCGTAAAGCATTTCATCAGGTCGACCTTGCTTTGGCAGATGACTGCAGTGTAAGCAGTTCTTCTGGGACGACAGCATTAACTGCCATGATCTTTGGAAGGCTTTTGATGGTAGCCAACACTGGTGATTGTCGAGCTGTTCTCTGCAGGAAGGGAGAGGCAATTGATATGTCCCAAGACCATAGGCCAATATATCCCTCGGAACGGAAGAGGGTTGAAGAGTTGGGGGGATATGTTGATGATGGGTATCTGAATGGTGTCTTATCAGTTTCCCGAGCCTTAGGTGACTGGGACATGAAGTTTCCTCGTGGTTCTGCTTCACCCCTTATCGCTGAACCAGAGTTCCGTAGGATGGATTTAACAGAGGAAGATGAGTTTCTCATTATAGGGTGTGATGGGATCTGGGATGTGATGTCAAGTCAGCATGCGGTTAGCCTTGTGCGCCGTGGCCTGCGGCGGCATGATGACCCAGAGCAGTGTGCCAAGGATCTTGTCATGGAGGCTCTTCGCCTCAACACGTTTGATAATCTTACTGTAATTGTTGTATGCTTCTCTTCTCTTGATCAGCGGGAACCATTATCACCGCCTCGGGAACGGAGATTTAGGTGTTGCAGCCTCTCTGCTGAGGCTCTCTGTAGCCTGAGGAGCTTGTTGGATACAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

395

Amino Acids

43.72

Weight (kDa)

5.04

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 97 - 349 2e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62260 AT3G62260 AT3G62260
fragaria_vesca FvH4_1g26700 FvH4_1g26700
malus_domestica MD09G1287100.v1.1
prunus_persica Prupe.3G006100_v2.0.a1
pyrus_communis pycom09g19420 pycom17g27730
rosa_chinensis RchiOBHm_Chr3g0486881
rosa_laevigata RLG00000023049
rosa_multiflora Rmu_sc0003470.1_g000010
rosa_roxburghii Rroxscaffold_6G00395430
rosa_rugosa Rorug03G0228500
rosa_samantha Rh3AG277700 Rh3BG313500 Rh3CG312000 Rh3DG308300
rosa_wichuraiana Rw3G024660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 543
AccII CGCG 1 cut(s) 289
AciI CCGC 8 cut(s) 29, 200, 287, 947, 973, 976, 1087, 1103
AclI AACGTT 2 cut(s) 36, 63
AclWI GGATC 3 cut(s) 352, 926, 1014
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 3 cut(s) 134, 242, 492
AcuI CTGAAG 1 cut(s) 429
AfiI CCNNNNNNNGG 4 cut(s) 130, 553, 723, 871
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 2 cut(s) 77, 737
AjnI CCWGG 3 cut(s) 237, 251, 339
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AloI GAACNNNNNNTCC 2 cut(s) 846, 878
AluBI AGCT 7 cut(s) 14, 270, 419, 476, 508, 656, 1167
AluI AGCT 7 cut(s) 14, 270, 419, 476, 508, 656, 1167
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 145, 436
AlwI GGATC 3 cut(s) 352, 926, 1014
Ama87I CYCGRG 4 cut(s) 471, 509, 792, 1107
AoxI GGCC 4 cut(s) 6, 125, 704, 967
ApeKI GCWGC 8 cut(s) 149, 152, 155, 158, 161, 413, 416, 1131
ApoI RAATTY 3 cut(s) 134, 242, 492
Asp700I GAANNNNTTC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 2 cut(s) 284, 404
AsuHPI GGTGA 4 cut(s) 656, 815, 831, 1092
AsuII TTCGAA 1 cut(s) 427
AvaI CYCGRG 4 cut(s) 471, 509, 792, 1107
AvaII GGWCC 2 cut(s) 284, 404
AxyI CCTNAGG 2 cut(s) 799, 1160
BanII GRGCYC 1 cut(s) 510
BauI CACGAG 1 cut(s) 825
Bbv12I GWGCWC 1 cut(s) 510
BbvCI CCTCAGC 1 cut(s) 1143
BbvI GCAGC 8 cut(s) 136, 139, 142, 145, 148, 425, 428, 1143
BccI CCATC 4 cut(s) 622, 755, 865, 908
BceAI ACGGC 1 cut(s) 948
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 3 cut(s) 239, 253, 341
BciVI GTATCC 1 cut(s) 1168
BclI TGATCA 1 cut(s) 1081
BcoDI GTCTC 2 cut(s) 145, 436
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 3 cut(s) 565, 664, 1153
BfuI GTATCC 1 cut(s) 1168
Bme1390I CCNGG 3 cut(s) 239, 253, 341
Bme18I GGWCC 2 cut(s) 284, 404
BmeT110I CYCGRG 4 cut(s) 471, 509, 792, 1107
BmgT120I GGNCC 2 cut(s) 284, 404
BmiI GGNNCC 3 cut(s) 222, 257, 1092
BmrFI CCNGG 3 cut(s) 239, 253, 341
BmrI ACTGGG 1 cut(s) 817
BmsI GCATC 1 cut(s) 98
BmuI ACTGGG 1 cut(s) 817
BoxI GACNNNNGTC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 645, 677
Bpu10I CCTNAGC 1 cut(s) 1143
Bpu14I TTCGAA 1 cut(s) 427
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 765
BsaJI CCNNGG 6 cut(s) 251, 340, 717, 964, 1002, 1106
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 553, 723, 871
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 2 cut(s) 646, 812
Bse21I CCTNAGG 2 cut(s) 799, 1160
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 3 cut(s) 239, 253, 341
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 6 cut(s) 251, 340, 717, 964, 1002, 1106
BseGI GGATG 2 cut(s) 876, 931
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 4 cut(s) 130, 553, 723, 871
BseMII CTCAG 3 cut(s) 513, 1134, 1151
BseNI ACTGG 2 cut(s) 646, 812
BseRI GAGGAG 2 cut(s) 518, 1177
BseXI GCAGC 8 cut(s) 136, 139, 142, 145, 148, 425, 428, 1143
Bsh1236I CGCG 1 cut(s) 289
BshFI GGCC 4 cut(s) 8, 127, 706, 969
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 4 cut(s) 471, 509, 792, 1107
BsiSI CCGG 1 cut(s) 124
BslFI GGGAC 3 cut(s) 601, 676, 824
BslI CCNNNNNNNGG 4 cut(s) 130, 553, 723, 871
BsmAI GTCTC 2 cut(s) 145, 436
BsmFI GGGAC 3 cut(s) 601, 676, 824
BsnI GGCC 4 cut(s) 8, 127, 706, 969
BsoBI CYCGRG 4 cut(s) 471, 509, 792, 1107
Bsp119I TTCGAA 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
BspACI CCGC 8 cut(s) 29, 200, 287, 947, 973, 976, 1087, 1103
BspANI GGCC 4 cut(s) 8, 127, 706, 969
BspCNI CTCAG 3 cut(s) 512, 1135, 1152
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 222, 257, 1092
BspMAI CTGCAG 2 cut(s) 569, 668
BspPI GGATC 3 cut(s) 352, 926, 1014
BspQI GCTCTTC 1 cut(s) 1029
BspT104I TTCGAA 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 2 cut(s) 646, 812
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 6 cut(s) 251, 340, 717, 964, 1002, 1106
BssMI GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 1 cut(s) 1002
Bst2BI CACGAG 1 cut(s) 825
Bst2UI CCWGG 3 cut(s) 239, 253, 341
Bst4CI ACNGT 1 cut(s) 1054
Bst6I CTCTTC 4 cut(s) 722, 732, 1029, 1078
BstAPI GCANNNNNTGC 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 125, 945, 971
BstDEI CTNAG 5 cut(s) 15, 499, 799, 1143, 1160
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 2 cut(s) 876, 931
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 7 cut(s) 21, 328, 347, 612, 921, 1009, 1084
BstMAI GTCTC 2 cut(s) 145, 436
BstMBI GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
BstMWI GCNNNNNNNGC 7 cut(s) 155, 158, 161, 573, 602, 960, 1164
BstNI CCWGG 3 cut(s) 239, 253, 341
BstNSI RCATGY 1 cut(s) 947
BstPAI GACNNNNGTC 1 cut(s) 117
BstSCI CCNGG 3 cut(s) 237, 251, 339
BstSFI CTRYAG 3 cut(s) 565, 664, 1153
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 8 cut(s) 136, 139, 142, 145, 148, 425, 428, 1143
BstX2I RGATCY 2 cut(s) 918, 1006
BstYI RGATCY 2 cut(s) 918, 1006
Bsu15I ATCGAT 1 cut(s) 92
Bsu36I CCTNAGG 2 cut(s) 799, 1160
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 4 cut(s) 8, 127, 706, 969
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 964
BtsCI GGATG 2 cut(s) 876, 931
BtsI GCAGTG 2 cut(s) 574, 1001
BtsIMutI CAGTG 3 cut(s) 574, 639, 1001
Cac8I GCNNGC 3 cut(s) 125, 945, 971
CfoI GCGC 1 cut(s) 963
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 284, 404
ClaI ATCGAT 1 cut(s) 92
CviAII CATG 8 cut(s) 22, 298, 398, 607, 814, 944, 980, 1015
DdeI CTNAG 5 cut(s) 15, 499, 799, 1143, 1160
DpnI GATC 7 cut(s) 20, 327, 346, 611, 920, 1008, 1083
DpnII GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 722, 732, 1029, 1078
EarI CTCTTC 4 cut(s) 722, 732, 1029, 1078
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 1002
Eco24I GRGCYC 1 cut(s) 510
Eco47I GGWCC 2 cut(s) 284, 404
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 429
Eco81I CCTNAGG 2 cut(s) 799, 1160
Eco88I CYCGRG 4 cut(s) 471, 509, 792, 1107
EcoICRI GAGCTC 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 237, 251, 339
EcoT14I CCWWGG 1 cut(s) 1002
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 1002
FaeI CATG 8 cut(s) 25, 301, 401, 610, 817, 947, 983, 1018
FalI AAGNNNNNCTT 2 cut(s) 565, 597
FaqI GGGAC 3 cut(s) 601, 676, 824
FatI CATG 8 cut(s) 21, 297, 397, 606, 813, 943, 979, 1014
FauI CCCGC 1 cut(s) 1080
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 1 cut(s) 543
FokI GGATG 2 cut(s) 883, 938
FriOI GRGCYC 1 cut(s) 510
FspBI CTAG 1 cut(s) 477
GlaI GCGC 1 cut(s) 962
HaeIII GGCC 4 cut(s) 8, 127, 706, 969
HapII CCGG 1 cut(s) 124
HhaI GCGC 1 cut(s) 963
Hin1II CATG 8 cut(s) 25, 301, 401, 610, 817, 947, 983, 1018
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 1 cut(s) 444
HpaII CCGG 1 cut(s) 124
HphI GGTGA 4 cut(s) 656, 815, 831, 1092
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 214, 502, 721, 771
Hpy188III TCNNGA 7 cut(s) 68, 131, 224, 511, 792, 1079, 1109
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 2 cut(s) 612, 664
HpyCH4III ACNGT 1 cut(s) 1054
HpyCH4IV ACGT 3 cut(s) 36, 63, 1037
HpyCH4V TGCA 4 cut(s) 89, 567, 666, 1131
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 158, 161, 573, 602, 960, 1164
HpyF3I CTNAG 5 cut(s) 15, 499, 799, 1143, 1160
HpySE526I ACGT 3 cut(s) 36, 63, 1037
Hsp92II CATG 8 cut(s) 25, 301, 401, 610, 817, 947, 983, 1018
HspAI GCGC 1 cut(s) 961
KroI GCCGGC 1 cut(s) 123
KroNI GCCGGC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 4 cut(s) 226, 261, 505, 1164
Lsp1109I GCAGC 8 cut(s) 136, 139, 142, 145, 148, 425, 428, 1143
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 36, 63, 1037
MaeIII GTNAC 1 cut(s) 803
MalI GATC 7 cut(s) 20, 327, 346, 611, 920, 1008, 1083
MboI GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
MboII GAAGA 9 cut(s) 89, 314, 324, 573, 739, 749, 899, 1016, 1065
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 2 cut(s) 918, 1006
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 6 cut(s) 134, 242, 463, 492, 681, 1056
MlyI GAGTC 1 cut(s) 438
MmeI TCCRAC 1 cut(s) 1152
MroNI GCCGGC 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 6 cut(s) 60, 167, 192, 486, 599, 878
MspA1I CMGCKG 2 cut(s) 200, 1087
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 3 cut(s) 239, 253, 341
MunI CAATTG 1 cut(s) 681
MvaI CCWGG 3 cut(s) 239, 253, 341
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 7 cut(s) 155, 158, 161, 573, 602, 960, 1164
NaeI GCCGGC 1 cut(s) 125
NdeII GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
NgoMIV GCCGGC 1 cut(s) 123
NlaIII CATG 8 cut(s) 25, 301, 401, 610, 817, 947, 983, 1018
NlaIV GGNNCC 3 cut(s) 222, 257, 1092
NmuCI GTSAC 1 cut(s) 803
NspI RCATGY 1 cut(s) 947
NspV TTCGAA 1 cut(s) 427
PaeI GCATGC 1 cut(s) 947
PaeR7I CTCGAG 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 1029
PdiI GCCGGC 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 246
PleI GAGTC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 438
PpuMI RGGWCCY 1 cut(s) 404
PshAI GACNNNNGTC 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 510
Psp1406I AACGTT 2 cut(s) 36, 63
Psp5II RGGWCCY 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 237, 251, 339
PspGI CCWGG 3 cut(s) 237, 251, 339
PspN4I GGNNCC 3 cut(s) 222, 257, 1092
PspPI GGNCC 2 cut(s) 284, 404
PspPPI RGGWCCY 1 cut(s) 404
PstI CTGCAG 2 cut(s) 569, 668
PsuI RGATCY 2 cut(s) 918, 1006
SacI GAGCTC 1 cut(s) 510
SalI GTCGAC 1 cut(s) 542
SapI GCTCTTC 1 cut(s) 1029
SaqAI TTAA 6 cut(s) 60, 167, 192, 486, 599, 878
Sau3AI GATC 7 cut(s) 18, 325, 344, 609, 918, 1006, 1081
Sau96I GGNCC 2 cut(s) 284, 404
SchI GAGTC 1 cut(s) 438
ScrFI CCNGG 3 cut(s) 239, 253, 341
SduI GDGCHC 1 cut(s) 510
SfaNI GCATC 1 cut(s) 98
SfcI CTRYAG 3 cut(s) 565, 664, 1153
Sfr274I CTCGAG 1 cut(s) 509
SfuI TTCGAA 1 cut(s) 427
SinI GGWCC 2 cut(s) 284, 404
SlaI CTCGAG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SphI GCATGC 1 cut(s) 947
Sse9I AATT 6 cut(s) 134, 242, 463, 492, 681, 1056
SsiI CCGC 8 cut(s) 29, 200, 287, 947, 973, 976, 1087, 1103
SspMI CTAG 1 cut(s) 477
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 237, 251, 339
StyI CCWWGG 1 cut(s) 1002
TaaI ACNGT 1 cut(s) 1054
TaiI ACGT 3 cut(s) 39, 66, 1040
TaqI TCGA 6 cut(s) 92, 173, 427, 510, 543, 652
TaqII GACCGA 1 cut(s) 129
TasI AATT 6 cut(s) 134, 242, 463, 492, 681, 1056
TauI GCSGC 2 cut(s) 976, 979
Tru1I TTAA 6 cut(s) 60, 167, 192, 486, 599, 878
Tru9I TTAA 6 cut(s) 60, 167, 192, 486, 599, 878
TscAI CASTG 3 cut(s) 574, 646, 1001
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 8 cut(s) 149, 152, 155, 158, 161, 413, 416, 1131
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 3 cut(s) 321, 524, 830
TspGWI ACGGA 6 cut(s) 248, 408, 512, 739, 854, 1129
TspRI CASTG 3 cut(s) 574, 646, 1001
VpaK11BI GGWCC 2 cut(s) 284, 404
XapI RAATTY 3 cut(s) 134, 242, 492
XceI RCATGY 1 cut(s) 947
XhoI CTCGAG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 543
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.