RLG00000023049

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012149
Physical Location & Seq
Reverse (-)
17281943 .. 17284287
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023049

Sequence Viewer

Length: 1176 bp
ATGGTGGCCGAAGCTAAGATCTTGTGTCCGCAGAACGTTGAAGTGTTTGCTAAAGGGATGAACGTTCGTGAGGTTGAAGATGTAGTTGCATCGATTTCGCCCAAGAGTTTTGACCGAAGTCGCCCTCTGGAATTTGTCTCCCCTGCTGCTGCTTTAATATCGAGCTCCCAACCTGATGTTAAACCAGCGGATAAAGTTTCAGACTTGGCTCCTGAACCAGCCGACTTGGAGTTCTTTCCCAGGCTCCGTTCTGGTAGCTTCGCTGACATTGGACCGCGTAGATACATGGAAGATGAACATATACTGATAGACGATCTATCTTCACACCTGGGATCACTCTTTAGTTTTCCCACCCCAAGTGCTTTTTATGGGGTATTTGACGGACATGGAGGACCTGAAGCAGCAGCTTATGTTCGAAAAAATGTGATGAGACTCTTGTTTGATGATGTCAATTTTCCCCGAGCTAGTGAAGTTAATAAAATTTTCTCAGAGGAGCTCGAGAATACTCTCCGTAAAGCATTTCATCAGGTAGACCTTGCTTTGGCAGATGACTGCAGTGTAAGCAGTTCTTCTGGGACGACAGCATTGACTGCCATGATCTTTGGAAGGCTTTTGATGGTAGCCAATACTGGTGATTGTCGAGCTGTTCTCTGCAGGAAGGGAGAGGCAATTGATATGTCCCAAGACCATAGGCCAATATATCCCTCGGAACGGAAGAGGGTTGAAGAATTGGGGGGATATGTTGATGATGGGTATCTGAATGGTGTCTTATCAGTTTCCCGAGCCTTAGGTGACTGGGACATGAAGTTTCCTCGTGGTTCTGCTTCACCCCTTATCGCCGAACCAGAGTTCCATAGGATGGATTTAACAGAGGAAGATGAATTTCTCATTATAGGGTGTGATGGGATCTGGGATGTGATGTCAAGTCAGCATGCGGTTAGCCTTGTGCGCCGTGGCCTGCGGCGGCATGATGACCCAGAGCAGTGTGCCAAGGATCTTGTCATGGAGGCTCTTCGCCTCAACACGTTTGATAATCTTACTGTAATTGTTGTATGCTTCTCTTCTCTTGATCAGCGGGAACCATTATCACCGCCTCGGGAACGGAGATTTAGGTGTTGCAGCCTCTCTGCTGAGGCTCTCTGTAGTCTGAGGAGCTTGCTGGATACAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

392

Amino Acids

43.32

Weight (kDa)

4.94

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 93 - 345 5.1e-65 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62260 AT3G62260 AT3G62260
fragaria_vesca FvH4_1g26700 FvH4_1g26700
malus_domestica MD09G1287100.v1.1
prunus_persica Prupe.3G006100_v2.0.a1
pyrus_communis pycom09g19420 pycom17g27730
rosa_chinensis RchiOBHm_Chr3g0486881
rosa_laevigata RLG00000023049
rosa_multiflora Rmu_sc0003470.1_g000010
rosa_roxburghii Rroxscaffold_6G00395430
rosa_rugosa Rorug03G0228500
rosa_samantha Rh3AG277700 Rh3BG313500 Rh3CG312000 Rh3DG308300
rosa_wichuraiana Rw3G024660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 859
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 277
AciI CCGC 8 cut(s) 29, 188, 275, 935, 961, 964, 1075, 1091
AclI AACGTT 2 cut(s) 36, 63
AclWI GGATC 3 cut(s) 340, 914, 1002
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 3 cut(s) 131, 480, 881
AcuI CTGAAG 1 cut(s) 417
AfiI CCNNNNNNNGG 3 cut(s) 541, 711, 859
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 3 cut(s) 41, 77, 725
AjnI CCWGG 2 cut(s) 239, 327
AloI GAACNNNNNNTCC 2 cut(s) 834, 866
AluBI AGCT 8 cut(s) 14, 165, 258, 407, 464, 496, 644, 1155
AluI AGCT 8 cut(s) 14, 165, 258, 407, 464, 496, 644, 1155
Alw21I GWGCWC 2 cut(s) 167, 498
Alw26I GTCTC 2 cut(s) 142, 424
AlwI GGATC 3 cut(s) 340, 914, 1002
Ama87I CYCGRG 4 cut(s) 459, 497, 780, 1095
AoxI GGCC 3 cut(s) 6, 692, 955
ApeKI GCWGC 5 cut(s) 146, 149, 401, 404, 1119
ApoI RAATTY 3 cut(s) 131, 480, 881
AspLEI GCGC 1 cut(s) 951
AspS9I GGNCC 2 cut(s) 272, 392
AsuHPI GGTGA 4 cut(s) 644, 803, 819, 1080
AsuII TTCGAA 1 cut(s) 415
AvaI CYCGRG 4 cut(s) 459, 497, 780, 1095
AvaII GGWCC 2 cut(s) 272, 392
AxyI CCTNAGG 1 cut(s) 787
BanII GRGCYC 2 cut(s) 167, 498
BauI CACGAG 1 cut(s) 813
Bbv12I GWGCWC 2 cut(s) 167, 498
BbvCI CCTCAGC 1 cut(s) 1131
BbvI GCAGC 5 cut(s) 133, 136, 413, 416, 1131
BccI CCATC 4 cut(s) 610, 743, 853, 896
BceAI ACGGC 1 cut(s) 936
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 2 cut(s) 241, 329
BciVI GTATCC 1 cut(s) 1156
BclI TGATCA 1 cut(s) 1069
BcoDI GTCTC 2 cut(s) 142, 424
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 3 cut(s) 553, 652, 1141
BfuI GTATCC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 18
BisI GCNGC 7 cut(s) 147, 150, 402, 405, 962, 965, 1120
BlsI GCNGC 7 cut(s) 148, 151, 403, 406, 963, 966, 1121
Bme1390I CCNGG 2 cut(s) 241, 329
Bme18I GGWCC 2 cut(s) 272, 392
BmeT110I CYCGRG 4 cut(s) 459, 497, 780, 1095
BmgT120I GGNCC 2 cut(s) 272, 392
BmiI GGNNCC 3 cut(s) 210, 245, 1080
BmrFI CCNGG 2 cut(s) 241, 329
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 1 cut(s) 98
BmuI ACTGGG 1 cut(s) 805
BoxI GACNNNNGTC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 633, 665
Bpu10I CCTNAGC 1 cut(s) 1131
Bpu14I TTCGAA 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 753
BsaJI CCNNGG 6 cut(s) 239, 328, 705, 952, 990, 1094
Bsc4I CCNNNNNNNGG 3 cut(s) 541, 711, 859
Bse1I ACTGG 2 cut(s) 634, 800
Bse21I CCTNAGG 1 cut(s) 787
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 2 cut(s) 241, 329
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 6 cut(s) 239, 328, 705, 952, 990, 1094
BseGI GGATG 3 cut(s) 63, 864, 919
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 3 cut(s) 541, 711, 859
BseMII CTCAG 3 cut(s) 501, 1122, 1139
BseNI ACTGG 2 cut(s) 634, 800
BseRI GAGGAG 2 cut(s) 506, 1165
BseXI GCAGC 5 cut(s) 133, 136, 413, 416, 1131
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 8, 694, 957
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 2 cut(s) 167, 498
BsiHKCI CYCGRG 4 cut(s) 459, 497, 780, 1095
BslFI GGGAC 3 cut(s) 589, 664, 812
BslI CCNNNNNNNGG 3 cut(s) 541, 711, 859
BsmAI GTCTC 2 cut(s) 142, 424
BsmFI GGGAC 3 cut(s) 589, 664, 812
BsnI GGCC 3 cut(s) 8, 694, 957
BsoBI CYCGRG 4 cut(s) 459, 497, 780, 1095
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 167, 498
Bsp143I GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
BspACI CCGC 8 cut(s) 29, 188, 275, 935, 961, 964, 1075, 1091
BspANI GGCC 3 cut(s) 8, 694, 957
BspCNI CTCAG 3 cut(s) 500, 1123, 1140
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 210, 245, 1080
BspMAI CTGCAG 2 cut(s) 557, 656
BspPI GGATC 3 cut(s) 340, 914, 1002
BspQI GCTCTTC 1 cut(s) 1017
BspT104I TTCGAA 1 cut(s) 415
BsrI ACTGG 2 cut(s) 634, 800
BssECI CCNNGG 6 cut(s) 239, 328, 705, 952, 990, 1094
BssMI GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
BssSI CACGAG 1 cut(s) 813
BssT1I CCWWGG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 813
Bst2UI CCWGG 2 cut(s) 241, 329
Bst4CI ACNGT 1 cut(s) 1042
Bst6I CTCTTC 3 cut(s) 710, 1017, 1066
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 3 cut(s) 933, 959, 1157
BstDEI CTNAG 5 cut(s) 15, 487, 787, 1131, 1148
BstDSI CCRYGG 1 cut(s) 952
BstF5I GGATG 3 cut(s) 63, 864, 919
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 951
BstKTI GATC 7 cut(s) 21, 316, 335, 600, 909, 997, 1072
BstMAI GTCTC 2 cut(s) 142, 424
BstMBI GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 561, 590, 948
BstNI CCWGG 2 cut(s) 241, 329
BstNSI RCATGY 1 cut(s) 935
BstPAI GACNNNNGTC 1 cut(s) 117
BstSCI CCNGG 2 cut(s) 239, 327
BstSFI CTRYAG 3 cut(s) 553, 652, 1141
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 5 cut(s) 133, 136, 413, 416, 1131
BstX2I RGATCY 3 cut(s) 18, 906, 994
BstYI RGATCY 3 cut(s) 18, 906, 994
Bsu15I ATCGAT 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 787
BsuI GTATCC 1 cut(s) 1156
BsuRI GGCC 3 cut(s) 8, 694, 957
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 952
BtsCI GGATG 3 cut(s) 63, 864, 919
BtsI GCAGTG 2 cut(s) 562, 989
BtsIMutI CAGTG 2 cut(s) 562, 989
Cac8I GCNNGC 3 cut(s) 933, 959, 1157
CfoI GCGC 1 cut(s) 951
Cfr13I GGNCC 2 cut(s) 272, 392
ClaI ATCGAT 1 cut(s) 92
CviAII CATG 7 cut(s) 286, 386, 595, 802, 932, 968, 1003
DdeI CTNAG 5 cut(s) 15, 487, 787, 1131, 1148
DpnI GATC 7 cut(s) 20, 315, 334, 599, 908, 996, 1071
DpnII GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 3 cut(s) 710, 1017, 1066
EarI CTCTTC 3 cut(s) 710, 1017, 1066
Ecl136II GAGCTC 2 cut(s) 165, 496
Eco130I CCWWGG 1 cut(s) 990
Eco24I GRGCYC 2 cut(s) 167, 498
Eco47I GGWCC 2 cut(s) 272, 392
Eco53kI GAGCTC 2 cut(s) 165, 496
Eco57I CTGAAG 1 cut(s) 417
Eco81I CCTNAGG 1 cut(s) 787
Eco88I CYCGRG 4 cut(s) 459, 497, 780, 1095
EcoICRI GAGCTC 2 cut(s) 165, 496
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 2 cut(s) 239, 327
EcoT14I CCWWGG 1 cut(s) 990
EcoT38I GRGCYC 2 cut(s) 167, 498
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 7 cut(s) 289, 389, 598, 805, 935, 971, 1006
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FaqI GGGAC 3 cut(s) 589, 664, 812
FatI CATG 7 cut(s) 285, 385, 594, 801, 931, 967, 1002
FauI CCCGC 1 cut(s) 1068
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 7 cut(s) 147, 150, 402, 405, 962, 965, 1120
FokI GGATG 3 cut(s) 70, 871, 926
FriOI GRGCYC 2 cut(s) 167, 498
Fsp4HI GCNGC 7 cut(s) 147, 150, 402, 405, 962, 965, 1120
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 950
GluI GCNGC 7 cut(s) 147, 150, 402, 405, 962, 965, 1120
HaeIII GGCC 3 cut(s) 8, 694, 957
HhaI GCGC 1 cut(s) 951
Hin1II CATG 7 cut(s) 289, 389, 598, 805, 935, 971, 1006
Hin6I GCGC 1 cut(s) 949
HinP1I GCGC 1 cut(s) 949
HinfI GANTC 1 cut(s) 432
HphI GGTGA 4 cut(s) 644, 803, 819, 1080
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 5 cut(s) 202, 490, 709, 759, 1149
Hpy188III TCNNGA 7 cut(s) 68, 128, 212, 499, 780, 1067, 1097
Hpy8I GTNNAC 1 cut(s) 532
HpyAV CCTTC 2 cut(s) 600, 652
HpyCH4III ACNGT 1 cut(s) 1042
HpyCH4IV ACGT 3 cut(s) 36, 63, 1025
HpyCH4V TGCA 4 cut(s) 89, 555, 654, 1119
HpyF10VI GCNNNNNNNGC 3 cut(s) 561, 590, 948
HpyF3I CTNAG 5 cut(s) 15, 487, 787, 1131, 1148
HpySE526I ACGT 3 cut(s) 36, 63, 1025
Hsp92II CATG 7 cut(s) 289, 389, 598, 805, 935, 971, 1006
HspAI GCGC 1 cut(s) 949
Ksp22I TGATCA 1 cut(s) 1069
Kzo9I GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 5 cut(s) 170, 214, 249, 493, 1152
Lsp1109I GCAGC 5 cut(s) 133, 136, 413, 416, 1131
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 465
MaeII ACGT 3 cut(s) 36, 63, 1025
MaeIII GTNAC 1 cut(s) 791
MalI GATC 7 cut(s) 20, 315, 334, 599, 908, 996, 1071
MboI GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
MboII GAAGA 9 cut(s) 89, 302, 312, 561, 727, 737, 887, 1004, 1053
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 3 cut(s) 18, 906, 994
MhlI GDGCHC 2 cut(s) 167, 498
MluCI AATT 7 cut(s) 131, 451, 480, 669, 728, 881, 1044
MlyI GAGTC 1 cut(s) 426
MseI TTAA 4 cut(s) 155, 180, 474, 866
MspA1I CMGCKG 2 cut(s) 188, 1075
MspR9I CCNGG 2 cut(s) 241, 329
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 2 cut(s) 241, 329
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 3 cut(s) 561, 590, 948
NdeII GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
NlaIII CATG 7 cut(s) 289, 389, 598, 805, 935, 971, 1006
NlaIV GGNNCC 3 cut(s) 210, 245, 1080
NmuCI GTSAC 1 cut(s) 791
NspI RCATGY 1 cut(s) 935
NspV TTCGAA 1 cut(s) 415
PaeI GCATGC 1 cut(s) 935
PaeR7I CTCGAG 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 1017
PflMI CCANNNNNTGG 1 cut(s) 859
PkrI GCNGC 7 cut(s) 148, 151, 403, 406, 963, 966, 1121
PleI GAGTC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 426
PpuMI RGGWCCY 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 117
Psp124BI GAGCTC 2 cut(s) 167, 498
Psp1406I AACGTT 2 cut(s) 36, 63
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 239, 327
PspGI CCWGG 2 cut(s) 239, 327
PspN4I GGNNCC 3 cut(s) 210, 245, 1080
PspPI GGNCC 2 cut(s) 272, 392
PspPPI RGGWCCY 1 cut(s) 392
PstI CTGCAG 2 cut(s) 557, 656
PsuI RGATCY 3 cut(s) 18, 906, 994
SacI GAGCTC 2 cut(s) 167, 498
SapI GCTCTTC 1 cut(s) 1017
SaqAI TTAA 4 cut(s) 155, 180, 474, 866
SatI GCNGC 7 cut(s) 147, 150, 402, 405, 962, 965, 1120
Sau3AI GATC 7 cut(s) 18, 313, 332, 597, 906, 994, 1069
Sau96I GGNCC 2 cut(s) 272, 392
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 2 cut(s) 241, 329
SduI GDGCHC 2 cut(s) 167, 498
SfaNI GCATC 1 cut(s) 98
SfcI CTRYAG 3 cut(s) 553, 652, 1141
Sfr274I CTCGAG 1 cut(s) 497
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 2 cut(s) 272, 392
SlaI CTCGAG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SphI GCATGC 1 cut(s) 935
Sse9I AATT 7 cut(s) 131, 451, 480, 669, 728, 881, 1044
SsiI CCGC 8 cut(s) 29, 188, 275, 935, 961, 964, 1075, 1091
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 2 cut(s) 167, 498
StyD4I CCNGG 2 cut(s) 239, 327
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 1 cut(s) 1042
TaiI ACGT 3 cut(s) 39, 66, 1028
TaqI TCGA 5 cut(s) 92, 161, 415, 498, 640
TaqII GACCGA 1 cut(s) 129
TasI AATT 7 cut(s) 131, 451, 480, 669, 728, 881, 1044
TauI GCSGC 2 cut(s) 964, 967
Tru1I TTAA 4 cut(s) 155, 180, 474, 866
Tru9I TTAA 4 cut(s) 155, 180, 474, 866
TscAI CASTG 2 cut(s) 562, 989
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 5 cut(s) 146, 149, 401, 404, 1119
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 5 cut(s) 74, 309, 512, 818, 894
TspGWI ACGGA 5 cut(s) 236, 396, 500, 727, 1117
TspRI CASTG 2 cut(s) 562, 989
Van91I CCANNNNNTGG 1 cut(s) 859
VpaK11BI GGWCC 2 cut(s) 272, 392
XapI RAATTY 3 cut(s) 131, 480, 881
XceI RCATGY 1 cut(s) 935
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.