pycom10g00400

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013601
Physical Location & Seq
Forward (+)
448148 .. 449078
931 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGGAATGGTCTCCTCAAGCTGCCTTGAAAGCTTACTTAGATACTCTTCAGCTGTGCAAGTTTCACAATGATCAAGACTTGGTGCATGGAAACACATGTAGTGGTGGTATTATAGAACCAAAATGCATGGAATTTATATCAGCTTTAGCAGCTGGAAAGCGAGCAAGGTTAATGTTGCAAGTCACATCTGAAGGCGTAACACCGCTGACGGTTTCGCTTGCGGTGGCTGCAAAGCAAAGTGGAGGCCGACTGATCGTGTGCATTAATAATGACGACGACGACGATCATACGGAGAAGATTGGTAAAACCCTACTTGTGGAAAACGGTCTCGACAACGTCATTGAATATGTATATGAAACTGATCCTTGTAGAATGGTGAAGCATTTCAAGCATGTTGATTTTGCGGCTGTGGACTGCAAGTTTAAAGATTACTTGAAACTTTTGAAGGTTATGAATCTCAACCCAAATGGGAGTATAGTGGTGAGAAACAATATTGGTGTGACTGTTAATGGAGGTCCAATTCTCGATCAAGTTTTCAAGGAAAAGAAGGGACTTGAATATGTGACCTTACCTTTAGGAGAAGGAATGGAGTTCACAAGATTCAGATCCAAGGGCAAGTGCAAAACCCAGAGATATAAGAGATTTCATGTAACATTTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

222

Amino Acids

24.74

Weight (kDa)

8.11

Isoelectric Point (pI)

20.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 3 - 211 3.5e-22 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013601)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00920
malus_domestica MD10G1005000.v1.1
prunus_persica Prupe.8G007800_v2.0.a1
pyrus_communis pycom10g00400
rosa_chinensis RchiOBHm_Chr6g0251271
rosa_laevigata RLG00000015468
rosa_multiflora Rmu_co8193732.1_g000001 Rmu_sc0001637.1_g000006 Rmu_sc0015396.1_g000007
rosa_roxburghii Rroxscaffold_7G00216860
rosa_rugosa Rorug05G0501400
rosa_samantha Rh6AG010800 Rh6BG001200 Rh6CG007800 Rh6DG008200
rosa_wichuraiana Rw6G001040 Rw6G001150 Rw6G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 203, 221, 404
AclWI GGATC 2 cut(s) 356, 600
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 2 cut(s) 32, 210
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 8 cut(s) 28, 344, 388, 436, 445, 538, 557, 659
AluBI AGCT 5 cut(s) 20, 32, 52, 143, 152
AluI AGCT 5 cut(s) 20, 32, 52, 143, 152
Alw26I GTCTC 2 cut(s) 15, 332
AlwI GGATC 2 cut(s) 356, 600
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 3 cut(s) 20, 149, 227
ApoI RAATTY 1 cut(s) 131
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 515
AsuHPI GGTGA 2 cut(s) 388, 493
AvaII GGWCC 1 cut(s) 515
BbvI GCAGC 3 cut(s) 7, 161, 214
BclI TGATCA 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 15, 332
BisI GCNGC 4 cut(s) 21, 150, 228, 405
BlsI GCNGC 4 cut(s) 22, 151, 229, 406
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 604
BsaI GGTCTC 2 cut(s) 15, 332
BsaJI CCNNGG 1 cut(s) 609
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 604
BseDI CCNNGG 1 cut(s) 609
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 1 cut(s) 316
BseXI GCAGC 3 cut(s) 7, 161, 214
BshFI GGCC 1 cut(s) 246
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 15, 332
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 2 cut(s) 15, 332
Bsp143I GATC 6 cut(s) 70, 252, 283, 361, 526, 605
BspACI CCGC 3 cut(s) 203, 221, 404
BspANI GGCC 1 cut(s) 246
BspPI GGATC 2 cut(s) 356, 600
BspTNI GGTCTC 2 cut(s) 15, 332
BssECI CCNNGG 1 cut(s) 609
BssMI GATC 6 cut(s) 70, 252, 283, 361, 526, 605
BssT1I CCWWGG 1 cut(s) 609
Bst4CI ACNGT 3 cut(s) 211, 326, 505
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 162, 219
BstDEI CTNAG 1 cut(s) 37
BstKTI GATC 6 cut(s) 73, 255, 286, 364, 529, 608
BstMAI GTCTC 2 cut(s) 15, 332
BstMBI GATC 6 cut(s) 70, 252, 283, 361, 526, 605
BstMWI GCNNNNNNNGC 4 cut(s) 29, 149, 227, 388
BstNSI RCATGY 2 cut(s) 99, 395
BstV1I GCAGC 3 cut(s) 7, 161, 214
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 1 cut(s) 246
Cac8I GCNNGC 2 cut(s) 162, 219
Cfr13I GGNCC 1 cut(s) 515
CviAII CATG 5 cut(s) 86, 96, 127, 392, 647
CviJI RGCY 8 cut(s) 20, 32, 52, 143, 152, 227, 246, 407
CviKI_1 RGCY 8 cut(s) 20, 32, 52, 143, 152, 227, 246, 407
DdeI CTNAG 1 cut(s) 37
DpnI GATC 6 cut(s) 72, 254, 285, 363, 528, 607
DpnII GATC 6 cut(s) 70, 252, 283, 361, 526, 605
DraI TTTAAA 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 609
Eco31I GGTCTC 2 cut(s) 15, 332
Eco47I GGWCC 1 cut(s) 515
Eco57I CTGAAG 2 cut(s) 32, 210
EcoT14I CCWWGG 1 cut(s) 609
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 5 cut(s) 89, 99, 130, 395, 650
FaqI GGGAC 1 cut(s) 564
FatI CATG 5 cut(s) 85, 95, 126, 391, 646
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 21, 150, 228, 405
Fsp4HI GCNGC 4 cut(s) 21, 150, 228, 405
GluI GCNGC 4 cut(s) 21, 150, 228, 405
HaeIII GGCC 1 cut(s) 246
Hin1II CATG 5 cut(s) 89, 99, 130, 395, 650
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 2 cut(s) 454, 600
HphI GGTGA 2 cut(s) 388, 493
Hpy166II GTNNAC 2 cut(s) 412, 594
Hpy188I TCNGA 2 cut(s) 190, 605
Hpy188III TCNNGA 3 cut(s) 74, 329, 524
Hpy8I GTNNAC 2 cut(s) 412, 594
Hpy99I CGWCG 3 cut(s) 278, 281, 284
HpyAV CCTTC 4 cut(s) 185, 439, 541, 575
HpyCH4III ACNGT 3 cut(s) 211, 326, 505
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 8 cut(s) 57, 85, 126, 178, 230, 261, 417, 621
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 149, 227, 388
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 5 cut(s) 89, 99, 130, 395, 650
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 6 cut(s) 70, 252, 283, 361, 526, 605
LpnPI CCDG 2 cut(s) 138, 641
Lsp1109I GCAGC 3 cut(s) 7, 161, 214
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 5 cut(s) 181, 196, 499, 562, 649
MalI GATC 6 cut(s) 72, 254, 285, 363, 528, 607
MboI GATC 6 cut(s) 70, 252, 283, 361, 526, 605
MboII GAAGA 2 cut(s) 38, 307
MflI RGATCY 1 cut(s) 605
MluCI AATT 3 cut(s) 131, 519, 661
MnlI CCTC 3 cut(s) 24, 236, 506
Mph1103I ATGCAT 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 5 cut(s) 170, 264, 423, 507, 664
MspA1I CMGCKG 3 cut(s) 52, 152, 205
MwoI GCNNNNNNNGC 4 cut(s) 29, 149, 227, 388
NdeII GATC 6 cut(s) 70, 252, 283, 361, 526, 605
NlaIII CATG 5 cut(s) 89, 99, 130, 395, 650
NmuCI GTSAC 3 cut(s) 181, 499, 562
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 2 cut(s) 99, 395
PciI ACATGT 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 454, 600
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 22, 151, 229, 406
PscI ACATGT 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 264
PspPI GGNCC 1 cut(s) 515
PsuI RGATCY 1 cut(s) 605
PsyI GACNNNGTC 1 cut(s) 335
PvuII CAGCTG 2 cut(s) 52, 152
SaqAI TTAA 5 cut(s) 170, 264, 423, 507, 664
SatI GCNGC 4 cut(s) 21, 150, 228, 405
Sau3AI GATC 6 cut(s) 70, 252, 283, 361, 526, 605
Sau96I GGNCC 1 cut(s) 515
SinI GGWCC 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
Sse9I AATT 3 cut(s) 131, 519, 661
SsiI CCGC 3 cut(s) 203, 221, 404
SspI AATATT 1 cut(s) 493
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 3 cut(s) 211, 326, 505
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 330, 525
TasI AATT 3 cut(s) 131, 519, 661
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 2 cut(s) 454, 600
Tru1I TTAA 5 cut(s) 170, 264, 423, 507, 664
Tru9I TTAA 5 cut(s) 170, 264, 423, 507, 664
TseFI GTSAC 3 cut(s) 181, 499, 562
TseI GCWGC 3 cut(s) 20, 149, 227
Tsp45I GTSAC 3 cut(s) 181, 499, 562
TspDTI ATGAA 3 cut(s) 369, 467, 635
TspGWI ACGGA 1 cut(s) 305
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 515
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 1 cut(s) 131
XceI RCATGY 2 cut(s) 99, 395
XmnI GAANNNNTTC 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.