Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007989

Physical Location & Seq

Genomic Coordinates Reverse (-)

3974187 .. 3984010

Gene Structure 9824 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGGACTCCATCGAGTCGCGGTGGGTCTTTCAGGACGAGGACGACTCCGAAGTCGACGACGAGGATGCAGATACGGAGGAAGATGCGATGCGTCGGACGGTTCTCGATTCGGAGGATGATGAAGAGGAGGACGATAATGCTGAGCAGCGCTTGATTCGCACTGGCCCTCGCGTGGATTCCTTTGACGTCGAAGCGCTCGAGGTCCCCGGCGCTCACAGAAACGAATATGAGGATTTCAGTCTTGGAAGGAAAATAATAATTGCTTTTCAGACCCTTGGGGTTGTCTTTGGTGATGTTGGAACAAGTCCATTATATACGTTCAGTGTCATGTTTAGCAAAGCACCTATTAATGGAAATGAGGATGTTATTGGAGCAATGTCGCTAGTCCTATACACATTAATCTTGATCCCGTTGCTTAAGTATGTGCTGGTGGTTCTTTGGGCTAATGATGATGGTGAAGGTGGTACATTTGCTTTGTATTCATTGATCTGTCGACATGCTAAGGTCAGTCTTCTTCCCAATCAGCTTCCGTCAGATGCCCGGATGTCGAGCTTCAGGCTAAAGGTGCCATCTCCTGAACTTGAAAGATCTTTAAAAATCAAGGAAAGGCTTGAGGCTTCACTGACTCTGAAAAAGCTTCTTCTGATGTTAGTGCTTGTTGGTACTGCTATGGTGATAGCTGATGGGGTTGTTACACCAGCAATGTCAGTAGTATCAGCTGTTGGTGGTCTAAAGGTTGGAGTAGAGGCAATTGAGCAAGATGAAGTGGTGATGATTTCGGTTGCCTTTCTTGTTATTTTATTCAGTGTACAGAAATTTGGGACAAGCAAAGTTGGACTGGCTGTAGGCCCTGCCTTATTCATATGGTTTTGTTCCCTTGGTGGGATTGGGATTTACAACCTTGTTAAATATGACAGCAGTGTCTTAAAGGCATTCAATCCTGTTCACATCTATTATTTCTTCGAGAGAAACTCAACTAAGGCTTGGTATTCTCTTGGGGGTTGTCTTTTATGCGCAACAGGTTCAGAGGCAATGTTTGCGGATCTTTGCTATTTTTCTGTACGATCAGTCCAGCTTACATTCGTTTTTCTTGTTTTGCCCTGCCTTACGTTGGGTTATTTGGGTCAAGCCGCCTACCTTATGGAGAACCAAGATGGGGCTGAGCAAGCTTTCTTTTCTTCAATCCCAAGTGGTGTTTTCTGGCCTGTGTTGTTCATTGCTAATGTTGCTGCATTGATTGCTAGTCGTGCAATGACAACGGCCACTTTTTCATGCATAAAACAATCAATGGCACTTGGTTGTTTCCCTCGCCTTAAAATCATACATACCTCCCGGAAGTTCATGGGGCAAATTTATATCCCTGTCGTAAATTGGTTTCTGCTGGTGGTTTCCCTAGTGGCTATCTGCACTATTTCAAACATTGATGAGATTGGAAATGCATATGGCATTGCTGAGCTAGGAGTGATGATGATGACGACCATTTTAGTAACAATCGTTATGCTTCTTATATGGCAGATAAACATAATCATCGTGCTGAGTTTCTTAATCATTTTCCTGGGGTTGGAGTTGACCTTTTTCTCATCGGTTTTGTGTAGTGTGGGAGATGGAAGTTGGATTATTTTGGTCTTTGCCATAATTATGTTTTTTATAATGTTTATCTGGAACTATGGAAGCAAGCTTAAGTATGAAACCGAAGTCAAGCAAAAACTTTCAATGGATCTGATGCGAGAATTGGGTTGCAATCTTGGGACAATCAGAGCTCCAGGCATTGGCTTGCTTTACAATGAGCTGGTGAAGGGAATACCAGCAATCTTTGGCCATTTTCTGACCACACTTCCTGCAATCCACTCGATGATCATATTTGTTTGTATAAAGTATGTTCCAGTTCCTGTAGTGCCTCAAAGCGAAAGGTTCCTTTTTCGGCGTGTCTGTCCGAAAAACTACCACATATTTCGTTGTATTGCCAGGTATGGCTATAAGGATGTTCGTAAAGAAAATCACCAAACATTTGAACAGTTACTAATTGAGAGCCTTGAGAAATTCATACGTAGGGAAGCTCAGCAGCGGTCACTGGAGAGCGATGGGGATGATGGTGATACAGACTCAGAAGATGAGACCTCTGTCTCGAGGGTTCTTATAGCCCCCAATGGAAGTGTTTACTCACTTGGTGTTCCTTTTCTGGCTGAGTACAAGGAGATGAGCCGGCAGCCTGTATCAGAAGCAAGCACATCAGAGGTGAAGCCAGTGCTTCCAGAAGATCCAACAGTTTTTGATTCTGAGCAGAGTATGGATAGGGAACTATCATTTATACGAAAGGCCAAAGAATCGGGGGTTGTTTATCTTCTTGGTCACGGGGATATTAGGGCCAGGAAAGATTCATGGTTTATCAAGAAGTTAATCATCAATTACTTTTATGCTTTCTTGAGGAAGAACTGCAGGAGGGGCACTGCGAATTTGAGTGTGCCCCATTCGCATCTAATGCAGGTTGGGATGACATATATGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004016 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009975 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0016829 GO:0016849 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

836

Amino Acids

93.37

Weight (kDa)

5.2

Isoelectric Point (pI)

41.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	88 - 573	2.9e-146	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	588 - 833	3.2e-24	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007989)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33530 AT4G33530 AT5G09400
fragaria_vesca	FvH4_2g11940 FvH4_2g11940 FvH4_2g11940 FvH4_2g11940
malus_domestica	MD05G1045000.v1.1 MD10G1051900.v1.1
prunus_persica	Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1
pyrus_communis	pycom05g03750 pycom10g03670
rosa_chinensis	RchiOBHm_Chr6g0271831
rosa_laevigata	RLG00000013711
rosa_multiflora	Rmu_sc0002396.1_g000028
rosa_roxburghii	Rroxscaffold_173G00434990 Rroxscaffold_7G00196740
rosa_rugosa	Rorug06G0063500
rosa_samantha	Rh6AG181700 Rh6BG185800 Rh6CG182300
rosa_wichuraiana	Rw6G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1649
AasI	GACNNNNNNGTC	2 cut(s)	50, 920
AatII	GACGTC	1 cut(s)	189
Acc16I	TGCGCA	1 cut(s)	1015
Acc36I	ACCTGC	1 cut(s)	2473
AccB1I	GGYRCC	1 cut(s)	565
AccB7I	CCANNNNNTGG	1 cut(s)	1769
AccI	GTMKAC	2 cut(s)	54, 493
AccII	CGCG	2 cut(s)	19, 171
AciI	CCGC	4 cut(s)	19, 1040, 1131, 2065
AclWI	GGATC	4 cut(s)	400, 1050, 1725, 2252
AcoI	YGGCCR	2 cut(s)	1260, 1816
AcsI	RAATTY	4 cut(s)	815, 1350, 2039, 2452
AcuI	CTGAAG	1 cut(s)	538
AcyI	GRCGYC	1 cut(s)	186
AdeI	CACNNNGTG	1 cut(s)	2168
AfaI	GTAC	5 cut(s)	466, 664, 810, 1062, 2189
AfeI	AGCGCT	2 cut(s)	149, 195
AfiI	CCNNNNNNNGG	7 cut(s)	172, 350, 882, 1111, 1156, 1561, 1769
AflII	CTTAAG	2 cut(s)	416, 1679
AgsI	TTSAA	6 cut(s)	584, 937, 1182, 1416, 1713, 2012
AjnI	CCWGG	4 cut(s)	1554, 1762, 1964, 2366
Alw21I	GWGCWC	1 cut(s)	1762
Alw26I	GTCTC	2 cut(s)	2108, 2128
AlwI	GGATC	4 cut(s)	400, 1050, 1725, 2252
AlwNI	CAGNNNCTG	1 cut(s)	1889
Ama87I	CYCGRG	2 cut(s)	197, 2125
Aor51HI	AGCGCT	2 cut(s)	149, 195
AoxI	GGCC	7 cut(s)	163, 847, 1202, 1260, 1816, 2316, 2364
ApeKI	GCWGC	4 cut(s)	145, 1229, 2062, 2206
ApoI	RAATTY	4 cut(s)	815, 1350, 2039, 2452
AseI	ATTAAT	2 cut(s)	348, 398
AspLEI	GCGC	4 cut(s)	150, 196, 212, 1016
AspS9I	GGNCC	4 cut(s)	164, 202, 848, 2364
AsuC2I	CCSGG	3 cut(s)	207, 541, 1333
AsuHPI	GGTGA	8 cut(s)	302, 467, 685, 781, 1804, 1991, 2105, 2248
AvaI	CYCGRG	2 cut(s)	197, 2125
AvaII	GGWCC	1 cut(s)	202
BaeGI	GKGCMC	2 cut(s)	2447, 2466
BalI	TGGCCA	1 cut(s)	1818
BanI	GGYRCC	1 cut(s)	565
BanII	GRGCYC	1 cut(s)	1762
BbsI	GAAGAC	1 cut(s)	503
Bbv12I	GWGCWC	1 cut(s)	1762
BbvI	GCAGC	4 cut(s)	157, 1216, 2074, 2218
BccI	CCATC	8 cut(s)	17, 446, 577, 677, 1148, 1598, 2075, 2084
BceAI	ACGGC	1 cut(s)	1275
BcgI	CGANNNNNNTGC	4 cut(s)	47, 81, 1830, 1864
BciT130I	CCWGG	4 cut(s)	1556, 1764, 1966, 2368
BclI	TGATCA	1 cut(s)	1854
BcnI	CCSGG	3 cut(s)	207, 541, 1333
BcoDI	GTCTC	2 cut(s)	2108, 2128
BfaI	CTAG	4 cut(s)	383, 1242, 1394, 1457
BfmI	CTRYAG	3 cut(s)	843, 1890, 2434
BfoI	RGCGCY	3 cut(s)	151, 197, 213
BfrI	CTTAAG	2 cut(s)	416, 1679
BfuAI	ACCTGC	1 cut(s)	2473
BglII	AGATCT	1 cut(s)	587
BisI	GCNGC	5 cut(s)	146, 1131, 1230, 2063, 2207
BlpI	GCTNAGC	4 cut(s)	141, 1161, 1452, 2058
BlsI	GCNGC	5 cut(s)	147, 1132, 1231, 2064, 2208
Bme1390I	CCNGG	7 cut(s)	207, 541, 1333, 1556, 1764, 1966, 2368
Bme18I	GGWCC	1 cut(s)	202
BmeT110I	CYCGRG	2 cut(s)	197, 2125
BmgT120I	GGNCC	4 cut(s)	164, 202, 848, 2364
BmiI	GGNNCC	3 cut(s)	204, 567, 1913
BmrFI	CCNGG	7 cut(s)	207, 541, 1333, 1556, 1764, 1966, 2368
BmsI	GCATC	6 cut(s)	55, 73, 78, 526, 1713, 2482
BoxI	GACNNNNGTC	1 cut(s)	2120
BpiI	GAAGAC	1 cut(s)	503
BplI	GAGNNNNNCTC	4 cut(s)	29, 61, 956, 988
BpmI	CTGGAG	2 cut(s)	1746, 2093
Bpu10I	CCTNAGC	1 cut(s)	501
Bpu1102I	GCTNAGC	4 cut(s)	141, 1161, 1452, 2058
BpuEI	CTTGAG	3 cut(s)	632, 2054, 2443
BpuMI	CCSGG	3 cut(s)	207, 541, 1333
BsaAI	YACGTR	1 cut(s)	2048
BsaHI	GRCGYC	1 cut(s)	186
BsaI	GGTCTC	1 cut(s)	2108
BsaJI	CCNNGG	4 cut(s)	205, 274, 877, 1555
Bsc4I	CCNNNNNNNGG	7 cut(s)	172, 350, 882, 1111, 1156, 1561, 1769
Bse118I	RCCGGY	1 cut(s)	2202
Bse1I	ACTGG	5 cut(s)	166, 843, 1883, 2076, 2243
Bse3DI	GCAATG	6 cut(s)	381, 708, 1038, 1215, 1257, 1446
BseBI	CCWGG	4 cut(s)	1556, 1764, 1966, 2368
BseDI	CCNNGG	4 cut(s)	205, 274, 877, 1555
BseGI	GGATG	7 cut(s)	70, 121, 367, 549, 1987, 2092, 2496
BseLI	CCNNNNNNNGG	7 cut(s)	172, 350, 882, 1111, 1156, 1561, 1769
BseMI	GCAATG	6 cut(s)	381, 708, 1038, 1215, 1257, 1446
BseMII	CTCAG	8 cut(s)	132, 1152, 1443, 1526, 2072, 2118, 2175, 2268
BseNI	ACTGG	5 cut(s)	166, 843, 1883, 2076, 2243
BseRI	GAGGAG	1 cut(s)	140
BseSI	GKGCMC	2 cut(s)	2447, 2466
BseXI	GCAGC	4 cut(s)	157, 1216, 2074, 2218
BsgI	GTGCAG	1 cut(s)	1390
Bsh1236I	CGCG	2 cut(s)	19, 171
BshFI	GGCC	7 cut(s)	165, 849, 1204, 1262, 1818, 2318, 2366
BshNI	GGYRCC	1 cut(s)	565
BsiHKAI	GWGCWC	1 cut(s)	1762
BsiHKCI	CYCGRG	2 cut(s)	197, 2125
BsiSI	CCGG	4 cut(s)	207, 541, 1333, 2203
BslFI	GGGAC	3 cut(s)	188, 835, 1762
BslI	CCNNNNNNNGG	7 cut(s)	172, 350, 882, 1111, 1156, 1561, 1769
BsmAI	GTCTC	2 cut(s)	2108, 2128
BsmFI	GGGAC	3 cut(s)	188, 835, 1762
BsmI	GAATGC	1 cut(s)	932
BsnI	GGCC	7 cut(s)	165, 849, 1204, 1262, 1818, 2318, 2366
Bso31I	GGTCTC	1 cut(s)	2108
BsoBI	CYCGRG	2 cut(s)	197, 2125
Bsp1286I	GDGCHC	3 cut(s)	1762, 2447, 2466
Bsp1407I	TGTACA	1 cut(s)	808
Bsp143I	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
Bsp1720I	GCTNAGC	4 cut(s)	141, 1161, 1452, 2058
BspACI	CCGC	4 cut(s)	19, 1040, 1131, 2065
BspANI	GGCC	7 cut(s)	165, 849, 1204, 1262, 1818, 2318, 2366
BspCNI	CTCAG	8 cut(s)	133, 1153, 1444, 1527, 2071, 2117, 2176, 2269
BspFNI	CGCG	2 cut(s)	19, 171
BspLI	GGNNCC	3 cut(s)	204, 567, 1913
BspMAI	CTGCAG	1 cut(s)	2438
BspMI	ACCTGC	1 cut(s)	2473
BspPI	GGATC	4 cut(s)	400, 1050, 1725, 2252
BspT107I	GGYRCC	1 cut(s)	565
BspTI	CTTAAG	2 cut(s)	416, 1679
BspTNI	GGTCTC	1 cut(s)	2108
BsrDI	GCAATG	6 cut(s)	381, 708, 1038, 1215, 1257, 1446
BsrFI	RCCGGY	1 cut(s)	2202
BsrGI	TGTACA	1 cut(s)	808
BsrI	ACTGG	5 cut(s)	166, 843, 1883, 2076, 2243
BssAI	RCCGGY	1 cut(s)	2202
BssECI	CCNNGG	4 cut(s)	205, 274, 877, 1555
BssMI	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
BssNI	GRCGYC	1 cut(s)	186
BssT1I	CCWWGG	2 cut(s)	274, 877
Bst2UI	CCWGG	4 cut(s)	1556, 1764, 1966, 2368
Bst4CI	ACNGT	3 cut(s)	100, 2016, 2266
Bst6I	CTCTTC	1 cut(s)	117
BstACI	GRCGYC	1 cut(s)	186
BstAFI	CTTAAG	2 cut(s)	416, 1679
BstAPI	GCANNNNNTGC	3 cut(s)	1037, 1238, 2479
BstAUI	TGTACA	1 cut(s)	808
BstBAI	YACGTR	1 cut(s)	2048
BstC8I	GCNNGC	5 cut(s)	1167, 1676, 1775, 2204, 2224
BstF5I	GGATG	7 cut(s)	70, 121, 367, 549, 1987, 2092, 2496
BstFNI	CGCG	2 cut(s)	19, 171
BstH2I	RGCGCY	3 cut(s)	151, 197, 213
BstHHI	GCGC	4 cut(s)	150, 196, 212, 1016
BstKTI	GATC	8 cut(s)	408, 489, 590, 1045, 1067, 1720, 1857, 2260
BstMAI	GTCTC	2 cut(s)	2108, 2128
BstMBI	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
BstNI	CCWGG	4 cut(s)	1556, 1764, 1966, 2368
BstNSI	RCATGY	1 cut(s)	500
BstPAI	GACNNNNGTC	1 cut(s)	2120
BstSCI	CCNGG	7 cut(s)	205, 539, 1331, 1554, 1762, 1964, 2366
BstSFI	CTRYAG	3 cut(s)	843, 1890, 2434
BstSLI	GKGCMC	2 cut(s)	2447, 2466
BstSNI	TACGTA	1 cut(s)	2048
BstUI	CGCG	2 cut(s)	19, 171
BstV1I	GCAGC	4 cut(s)	157, 1216, 2074, 2218
BstV2I	GAAGAC	1 cut(s)	503
BstX2I	RGATCY	4 cut(s)	587, 1042, 1717, 2257
BstYI	RGATCY	4 cut(s)	587, 1042, 1717, 2257
BsuRI	GGCC	7 cut(s)	165, 849, 1204, 1262, 1818, 2318, 2366
BtgZI	GCGATG	2 cut(s)	101, 2094
BtsCI	GGATG	7 cut(s)	70, 121, 367, 549, 1987, 2092, 2496
BtsI	GCAGTG	2 cut(s)	925, 2445
BtsIMutI	CAGTG	8 cut(s)	159, 328, 620, 811, 925, 2069, 2250, 2445
BveI	ACCTGC	1 cut(s)	2473
Cac8I	GCNNGC	5 cut(s)	1167, 1676, 1775, 2204, 2224
CaiI	CAGNNNCTG	1 cut(s)	1889
CfoI	GCGC	4 cut(s)	150, 196, 212, 1016
Cfr10I	RCCGGY	1 cut(s)	2202
Cfr13I	GGNCC	4 cut(s)	164, 202, 848, 2364
CseI	GACGC	1 cut(s)	80
Csp6I	GTAC	5 cut(s)	465, 663, 809, 1061, 2188
CviAII	CATG	5 cut(s)	328, 497, 1272, 1342, 2379
CviQI	GTAC	5 cut(s)	465, 663, 809, 1061, 2188
DpnI	GATC	8 cut(s)	407, 488, 589, 1044, 1066, 1719, 1856, 2259
DpnII	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
DraI	TTTAAA	1 cut(s)	594
DraIII	CACNNNGTG	1 cut(s)	2168
DrdI	GACNNNNNNGTC	2 cut(s)	50, 920
DseDI	GACNNNNNNGTC	2 cut(s)	50, 920
EaeI	YGGCCR	2 cut(s)	1260, 1816
Eam1104I	CTCTTC	1 cut(s)	117
EarI	CTCTTC	1 cut(s)	117
Ecl136II	GAGCTC	1 cut(s)	1760
Eco105I	TACGTA	1 cut(s)	2048
Eco130I	CCWWGG	2 cut(s)	274, 877
Eco24I	GRGCYC	1 cut(s)	1762
Eco31I	GGTCTC	1 cut(s)	2108
Eco47I	GGWCC	1 cut(s)	202
Eco47III	AGCGCT	2 cut(s)	149, 195
Eco53kI	GAGCTC	1 cut(s)	1760
Eco57I	CTGAAG	1 cut(s)	538
Eco88I	CYCGRG	2 cut(s)	197, 2125
EcoICRI	GAGCTC	1 cut(s)	1760
EcoO109I	RGGNCCY	2 cut(s)	202, 848
EcoRII	CCWGG	4 cut(s)	1554, 1762, 1964, 2366
EcoT14I	CCWWGG	2 cut(s)	274, 877
EcoT22I	ATGCAT	2 cut(s)	1277, 1441
EcoT38I	GRGCYC	1 cut(s)	1762
ErhI	CCWWGG	2 cut(s)	274, 877
FaeI	CATG	5 cut(s)	331, 500, 1275, 1345, 2382
FaqI	GGGAC	3 cut(s)	188, 835, 1762
FatI	CATG	5 cut(s)	327, 496, 1271, 1341, 2378
FauNDI	CATATG	2 cut(s)	863, 1441
FbaI	TGATCA	1 cut(s)	1854
FblI	GTMKAC	2 cut(s)	54, 493
Fnu4HI	GCNGC	5 cut(s)	146, 1131, 1230, 2063, 2207
FokI	GGATG	7 cut(s)	77, 128, 374, 556, 1994, 2099, 2503
FriOI	GRGCYC	1 cut(s)	1762
Fsp4HI	GCNGC	5 cut(s)	146, 1131, 1230, 2063, 2207
FspBI	CTAG	4 cut(s)	383, 1242, 1394, 1457
FspI	TGCGCA	1 cut(s)	1015
GlaI	GCGC	4 cut(s)	149, 195, 211, 1015
GluI	GCNGC	5 cut(s)	146, 1131, 1230, 2063, 2207
GsuI	CTGGAG	2 cut(s)	1746, 2093
HaeII	RGCGCY	3 cut(s)	151, 197, 213
HaeIII	GGCC	7 cut(s)	165, 849, 1204, 1262, 1818, 2318, 2366
HapII	CCGG	4 cut(s)	207, 541, 1333, 2203
HgaI	GACGC	1 cut(s)	80
HhaI	GCGC	4 cut(s)	150, 196, 212, 1016
Hin1I	GRCGYC	1 cut(s)	186
Hin1II	CATG	5 cut(s)	331, 500, 1275, 1345, 2382
Hin6I	GCGC	4 cut(s)	148, 194, 210, 1014
HinP1I	GCGC	4 cut(s)	148, 194, 210, 1014
HincII	GTYRAC	3 cut(s)	55, 494, 1569
HindII	GTYRAC	3 cut(s)	55, 494, 1569
HindIII	AAGCTT	3 cut(s)	635, 1167, 1676
HpaII	CCGG	4 cut(s)	207, 541, 1333, 2203
HphI	GGTGA	8 cut(s)	302, 467, 685, 781, 1804, 1991, 2105, 2248
Hpy166II	GTNNAC	6 cut(s)	55, 494, 809, 946, 1569, 2158
Hpy8I	GTNNAC	6 cut(s)	55, 494, 809, 946, 1569, 2158
Hpy99I	CGWCG	4 cut(s)	59, 62, 96, 191
HpyAV	CCTTC	3 cut(s)	240, 452, 1789
HpyCH4III	ACNGT	3 cut(s)	100, 2016, 2266
HpyCH4IV	ACGT	4 cut(s)	186, 317, 1109, 2047
HpySE526I	ACGT	4 cut(s)	186, 317, 1109, 2047
Hsp92I	GRCGYC	1 cut(s)	186
Hsp92II	CATG	5 cut(s)	331, 500, 1275, 1345, 2382
HspAI	GCGC	4 cut(s)	148, 194, 210, 1014
KroI	GCCGGC	1 cut(s)	2202
KroNI	GCCGGC	1 cut(s)	2204
Ksp22I	TGATCA	1 cut(s)	1854
Kzo9I	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
LmnI	GCTCC	2 cut(s)	371, 1765
Lsp1109I	GCAGC	4 cut(s)	157, 1216, 2074, 2218
LweI	GCATC	6 cut(s)	55, 73, 78, 526, 1713, 2482
MaeI	CTAG	4 cut(s)	383, 1242, 1394, 1457
MaeII	ACGT	4 cut(s)	186, 317, 1109, 2047
MaeIII	GTNAC	5 cut(s)	691, 1486, 2016, 2067, 2348
MalI	GATC	8 cut(s)	407, 488, 589, 1044, 1066, 1719, 1856, 2259
MboI	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
MfeI	CAATTG	1 cut(s)	750
MflI	RGATCY	4 cut(s)	587, 1042, 1717, 2257
MhlI	GDGCHC	3 cut(s)	1762, 2447, 2466
MlsI	TGGCCA	1 cut(s)	1818
MluNI	TGGCCA	1 cut(s)	1818
MlyI	GAGTC	4 cut(s)	23, 38, 619, 2096
MmeI	TCCRAC	7 cut(s)	74, 277, 718, 814, 1542, 1592, 2285
Mox20I	TGGCCA	1 cut(s)	1818
Mph1103I	ATGCAT	2 cut(s)	1277, 1441
MroNI	GCCGGC	1 cut(s)	2202
MscI	TGGCCA	1 cut(s)	1818
MslI	CAYNNNNRTG	1 cut(s)	1637
Msp20I	TGGCCA	1 cut(s)	1818
MspA1I	CMGCKG	2 cut(s)	719, 2065
MspCI	CTTAAG	2 cut(s)	416, 1679
MspI	CCGG	4 cut(s)	207, 541, 1333, 2203
MspR9I	CCNGG	7 cut(s)	207, 541, 1333, 1556, 1764, 1966, 2368
MunI	CAATTG	1 cut(s)	750
Mva1269I	GAATGC	1 cut(s)	932
MvaI	CCWGG	4 cut(s)	1556, 1764, 1966, 2368
MvnI	CGCG	2 cut(s)	19, 171
NaeI	GCCGGC	1 cut(s)	2204
NciI	CCSGG	3 cut(s)	207, 541, 1333
NdeI	CATATG	2 cut(s)	863, 1441
NdeII	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
NgoMIV	GCCGGC	1 cut(s)	2202
NlaIII	CATG	5 cut(s)	331, 500, 1275, 1345, 2382
NlaIV	GGNNCC	3 cut(s)	204, 567, 1913
NmuCI	GTSAC	2 cut(s)	2067, 2348
NsbI	TGCGCA	1 cut(s)	1015
NsiI	ATGCAT	2 cut(s)	1277, 1441
NspI	RCATGY	1 cut(s)	500
PaeR7I	CTCGAG	2 cut(s)	197, 2125
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PctI	GAATGC	1 cut(s)	932
PdiI	GCCGGC	1 cut(s)	2204
PfeI	GAWTC	6 cut(s)	107, 154, 176, 2273, 2324, 2375
PflMI	CCANNNNNTGG	1 cut(s)	1769
PfoI	TCCNGGA	1 cut(s)	1331
PkrI	GCNGC	5 cut(s)	147, 1132, 1231, 2064, 2208
PleI	GAGTC	4 cut(s)	22, 38, 619, 2096
PpsI	GAGTC	4 cut(s)	22, 38, 619, 2096
Ppu21I	YACGTR	1 cut(s)	2048
PpuMI	RGGWCCY	1 cut(s)	202
PshAI	GACNNNNGTC	1 cut(s)	2120
PshBI	ATTAAT	2 cut(s)	348, 398
PsiI	TTATAA	1 cut(s)	1649
Psp124BI	GAGCTC	1 cut(s)	1762
Psp5II	RGGWCCY	1 cut(s)	202
Psp6I	CCWGG	4 cut(s)	1554, 1762, 1964, 2366
PspGI	CCWGG	4 cut(s)	1554, 1762, 1964, 2366
PspN4I	GGNNCC	3 cut(s)	204, 567, 1913
PspPI	GGNCC	4 cut(s)	164, 202, 848, 2364
PspPPI	RGGWCCY	1 cut(s)	202
PspXI	VCTCGAGB	1 cut(s)	197
PstI	CTGCAG	1 cut(s)	2438
PstNI	CAGNNNCTG	1 cut(s)	1889
PsuI	RGATCY	4 cut(s)	587, 1042, 1717, 2257
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	5 cut(s)	466, 664, 810, 1062, 2189
RsaNI	GTAC	5 cut(s)	465, 663, 809, 1061, 2188
RseI	CAYNNNNRTG	1 cut(s)	1637
SacI	GAGCTC	1 cut(s)	1762
SalI	GTCGAC	2 cut(s)	53, 492
SatI	GCNGC	5 cut(s)	146, 1131, 1230, 2063, 2207
Sau3AI	GATC	8 cut(s)	405, 486, 587, 1042, 1064, 1717, 1854, 2257
Sau96I	GGNCC	4 cut(s)	164, 202, 848, 2364
SchI	GAGTC	4 cut(s)	23, 38, 619, 2096
ScrFI	CCNGG	7 cut(s)	207, 541, 1333, 1556, 1764, 1966, 2368
SduI	GDGCHC	3 cut(s)	1762, 2447, 2466
SfaNI	GCATC	6 cut(s)	55, 73, 78, 526, 1713, 2482
SfcI	CTRYAG	3 cut(s)	843, 1890, 2434
Sfr274I	CTCGAG	2 cut(s)	197, 2125
SinI	GGWCC	1 cut(s)	202
SlaI	CTCGAG	2 cut(s)	197, 2125
SmiMI	CAYNNNNRTG	1 cut(s)	1637
SmlI	CTYRAG	7 cut(s)	197, 416, 611, 1679, 2033, 2125, 2422
SmoI	CTYRAG	7 cut(s)	197, 416, 611, 1679, 2033, 2125, 2422
SnaBI	TACGTA	1 cut(s)	2048
SsiI	CCGC	4 cut(s)	19, 1040, 1131, 2065
SspMI	CTAG	4 cut(s)	383, 1242, 1394, 1457
SstI	GAGCTC	1 cut(s)	1762
StyD4I	CCNGG	7 cut(s)	205, 539, 1331, 1554, 1762, 1964, 2366
StyI	CCWWGG	2 cut(s)	274, 877
TaaI	ACNGT	3 cut(s)	100, 2016, 2266
TaiI	ACGT	4 cut(s)	189, 320, 1112, 2050
TatI	WGTACW	2 cut(s)	808, 2187
TauI	GCSGC	1 cut(s)	1133
TfiI	GAWTC	6 cut(s)	107, 154, 176, 2273, 2324, 2375
TscAI	CASTG	8 cut(s)	166, 328, 627, 811, 925, 2076, 2250, 2452
TseFI	GTSAC	2 cut(s)	2067, 2348
TseI	GCWGC	4 cut(s)	145, 1229, 2062, 2206
Tsp45I	GTSAC	2 cut(s)	2067, 2348
TspGWI	ACGGA	2 cut(s)	89, 519
TspRI	CASTG	8 cut(s)	166, 328, 627, 811, 925, 2076, 2250, 2452
Van91I	CCANNNNNTGG	1 cut(s)	1769
Vha464I	CTTAAG	2 cut(s)	416, 1679
VpaK11BI	GGWCC	1 cut(s)	202
VspI	ATTAAT	2 cut(s)	348, 398
XapI	RAATTY	4 cut(s)	815, 1350, 2039, 2452
XceI	RCATGY	1 cut(s)	500
XhoI	CTCGAG	2 cut(s)	197, 2125
XmiI	GTMKAC	2 cut(s)	54, 493
XspI	CTAG	4 cut(s)	383, 1242, 1394, 1457
ZraI	GACGTC	1 cut(s)	187
Zsp2I	ATGCAT	2 cut(s)	1277, 1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.