pycom10g03680

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013343
Physical Location & Seq
Forward (+)
3985936 .. 3991205
5270 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 876 bp
ATGTTCGAAATGGTAGCATTAGCAGGTTTCTCAGCCCCTCCGAGGCTGAGCTTTTCATTGGGTTCTTGCCGCCGTAGTAACTCAACATCTCTTCCTTCATTGCCTCTCAGTCTCAGTCTCAGTCTCAGTCTCAGAAATTCGACGCCAAAGTCCACAGTCTCTGCCTTGCACACTCCGTACGGCGATTCTTTAGCAACTAGTGGATTATCAAGTAGAACTTGTGGGTCACCTCTAAAGCTTGACGGCAACGATTTTGGTGATACTAGTACAAGTTATGGTGCAATAGAAGCCAAAAAAGGGAATCCACCCATACTCCCAGCTGTGATGACCCCAGGTGGACCATTGGATCTCTCTTCTGTATTATTCAGGAACCGTATAATCTTCATTGGGCAGCCAGTCACTTCAGCGGTGGCTCAACGAGTCATATCCCAGCTTGTGACTTTGGCTACTATTGATGAGAATTCAGATATACTGGTGTATTTGAACTGCCCTGGTGGGAGTACATACTCTGTTTTGGCAATTTATGACTGCATGTCTTGGATCAAACCTAAGGTAGGAACAGTATGTTTTGGAGTAGCTGCAAGCCAAGGAGCACTTCTTCTTGCTGGGGGAGAAAAGGGAATGCGCTATGCGATGCCAAATGCACGAATCATGATACACCAACCACAGAGTGGTTGTGGGGGCCATGTGGAAGATGTGAGGCGCCAAGTGAACGAAGCAGTTCAAACTCGCCATAAAGTTGACAAAATGTATGTTGCGTTTACTGGGCAATCTCTAGAGAAAGTGCAACAGTACACTGAGAGGGATCGTTTCTTATCTGTTTCCGAGGCTATGGAATTCGGGCTTATTGATGGCGTGCTGGAAACCGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04112 ko04212 map04112 map04212
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

292

Amino Acids

31.14

Weight (kDa)

7.6

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 116 - 289 5.2e-67 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11750 AT1G11750
fragaria_vesca FvH4_2g13930
malus_domestica MD05G1045200.v1.1 MD10G1052100.v1.1
prunus_persica Prupe.8G061700_v2.0.a1 Prupe.8G061700_v2.0.a1
pyrus_communis pycom10g03680
rosa_chinensis RchiOBHm_Chr6g0275681
rosa_laevigata RLG00000013447
rosa_multiflora Rmu_sc0016068.1_g000003 Rmu_sc0018708.1_g000009
rosa_roxburghii Rroxscaffold_7G00193030
rosa_rugosa Rorug06G0095600
rosa_samantha Rh6AG207500 Rh6CG213500 Rh6DG203200
rosa_wichuraiana Rw6G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
Acc36I ACCTGC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 702
AccB7I CCANNNNNTGG 1 cut(s) 671
AciI CCGC 2 cut(s) 70, 407
AclWI GGATC 3 cut(s) 354, 548, 813
AcsI RAATTY 3 cut(s) 136, 460, 836
AcuI CTGAAG 1 cut(s) 387
AcyI GRCGYC 2 cut(s) 143, 703
AdeI CACNNNGTG 1 cut(s) 671
AfaI GTAC 4 cut(s) 179, 268, 502, 794
AfiI CCNNNNNNNGG 4 cut(s) 42, 297, 554, 671
AgsI TTSAA 2 cut(s) 484, 725
AhdI GACNNNNNGTC 1 cut(s) 532
AhlI ACTAGT 2 cut(s) 197, 263
AjnI CCWGG 2 cut(s) 331, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 5 cut(s) 51, 238, 320, 433, 578
AluI AGCT 5 cut(s) 51, 238, 320, 433, 578
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 5 cut(s) 116, 122, 128, 134, 163
AlwI GGATC 3 cut(s) 354, 548, 813
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 1 cut(s) 682
ApeKI GCWGC 2 cut(s) 391, 578
ApoI RAATTY 3 cut(s) 136, 460, 836
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 2 cut(s) 627, 705
AspS9I GGNCC 2 cut(s) 338, 682
AsuHPI GGTGA 2 cut(s) 219, 269
AsuII TTCGAA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 549
BanI GGYRCC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 2 cut(s) 403, 565
BccI CCATC 1 cut(s) 845
BceAI ACGGC 3 cut(s) 57, 196, 259
BciT130I CCWGG 2 cut(s) 333, 492
BcoDI GTCTC 5 cut(s) 116, 122, 128, 134, 163
BcuI ACTAGT 2 cut(s) 197, 263
BfaI CTAG 3 cut(s) 198, 264, 776
BfoI RGCGCY 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 3 cut(s) 70, 392, 579
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 3 cut(s) 71, 393, 580
Bme1390I CCNGG 2 cut(s) 333, 492
Bme18I GGWCC 1 cut(s) 338
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgT120I GGNCC 2 cut(s) 338, 682
BmiI GGNNCC 3 cut(s) 371, 683, 704
BmrFI CCNGG 2 cut(s) 333, 492
BmrI ACTGGG 1 cut(s) 774
BmsI GCATC 1 cut(s) 624
BmuI ACTGGG 1 cut(s) 774
Bpu1102I GCTNAGC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 6
BsaHI GRCGYC 2 cut(s) 143, 703
BsaJI CCNNGG 5 cut(s) 41, 331, 490, 586, 825
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 297, 554, 671
Bse1I ACTGG 3 cut(s) 395, 477, 769
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 98
BseBI CCWGG 2 cut(s) 333, 492
BseDI CCNNGG 5 cut(s) 41, 331, 490, 586, 825
BseLI CCNNNNNNNGG 4 cut(s) 42, 297, 554, 671
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 8 cut(s) 38, 45, 121, 127, 133, 139, 145, 789
BseNI ACTGG 3 cut(s) 395, 477, 769
BseXI GCAGC 2 cut(s) 403, 565
BseYI CCCAGC 3 cut(s) 316, 429, 605
BshFI GGCC 1 cut(s) 684
BshNI GGYRCC 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 595
BsiWI CGTACG 1 cut(s) 177
BslI CCNNNNNNNGG 4 cut(s) 42, 297, 554, 671
BsmAI GTCTC 5 cut(s) 116, 122, 128, 134, 163
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 1 cut(s) 684
Bsp119I TTCGAA 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 3 cut(s) 346, 540, 805
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 2 cut(s) 70, 407
BspANI GGCC 1 cut(s) 684
BspCNI CTCAG 8 cut(s) 39, 44, 120, 126, 132, 138, 144, 790
BspHI TCATGA 1 cut(s) 651
BspLI GGNNCC 3 cut(s) 371, 683, 704
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 3 cut(s) 354, 548, 813
BspT104I TTCGAA 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 702
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 3 cut(s) 395, 477, 769
BssECI CCNNGG 5 cut(s) 41, 331, 490, 586, 825
BssMI GATC 3 cut(s) 346, 540, 805
BssNI GRCGYC 2 cut(s) 143, 703
BssT1I CCWWGG 1 cut(s) 586
Bst2UI CCWGG 2 cut(s) 333, 492
Bst4CI ACNGT 4 cut(s) 157, 374, 562, 792
Bst6I CTCTTC 2 cut(s) 96, 358
BstACI GRCGYC 2 cut(s) 143, 703
BstBI TTCGAA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 583, 857
BstDEI CTNAG 9 cut(s) 31, 47, 107, 113, 119, 125, 131, 549, 798
BstEII GGTNACC 1 cut(s) 225
BstENI CCTNNNNNAGG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 706
BstHHI GCGC 2 cut(s) 627, 705
BstKTI GATC 3 cut(s) 349, 543, 808
BstMAI GTCTC 5 cut(s) 116, 122, 128, 134, 163
BstMBI GATC 3 cut(s) 346, 540, 805
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 2 cut(s) 333, 492
BstNSI RCATGY 1 cut(s) 535
BstPI GGTNACC 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 331, 490
BstV1I GCAGC 2 cut(s) 403, 565
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 549
BsuRI GGCC 1 cut(s) 684
BtgZI GCGATG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 2 cut(s) 583, 857
CaiI CAGNNNCTG 1 cut(s) 161
CciI TCATGA 1 cut(s) 651
CfoI GCGC 2 cut(s) 627, 705
Cfr13I GGNCC 2 cut(s) 338, 682
CseI GACGC 1 cut(s) 151
Csp6I GTAC 4 cut(s) 178, 267, 501, 793
CviAII CATG 3 cut(s) 532, 652, 686
CviQI GTAC 4 cut(s) 178, 267, 501, 793
DdeI CTNAG 9 cut(s) 31, 47, 107, 113, 119, 125, 131, 549, 798
DinI GGCGCC 1 cut(s) 704
DpnI GATC 3 cut(s) 348, 542, 807
DpnII GATC 3 cut(s) 346, 540, 805
DraIII CACNNNGTG 1 cut(s) 671
DrdI GACNNNNNNGTC 1 cut(s) 148
DriI GACNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 96, 358
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 2 cut(s) 96, 358
Eco130I CCWWGG 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 549
Eco91I GGTNACC 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoO65I GGTNACC 1 cut(s) 225
EcoRI GAATTC 2 cut(s) 460, 836
EcoRII CCWGG 2 cut(s) 331, 490
EcoT14I CCWWGG 1 cut(s) 586
EgeI GGCGCC 1 cut(s) 704
EheI GGCGCC 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 586
FaeI CATG 3 cut(s) 535, 655, 689
FalI AAGNNNNNCTT 4 cut(s) 202, 234, 579, 611
FatI CATG 3 cut(s) 531, 651, 685
Fnu4HI GCNGC 3 cut(s) 70, 392, 579
Fsp4HI GCNGC 3 cut(s) 70, 392, 579
FspBI CTAG 3 cut(s) 198, 264, 776
GlaI GCGC 2 cut(s) 626, 704
GluI GCNGC 3 cut(s) 70, 392, 579
GsaI CCCAGC 3 cut(s) 320, 433, 609
HaeII RGCGCY 1 cut(s) 706
HaeIII GGCC 1 cut(s) 684
HgaI GACGC 1 cut(s) 151
HhaI GCGC 2 cut(s) 627, 705
Hin1I GRCGYC 2 cut(s) 143, 703
Hin1II CATG 3 cut(s) 535, 655, 689
Hin6I GCGC 2 cut(s) 625, 703
HinP1I GCGC 2 cut(s) 625, 703
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 4 cut(s) 185, 301, 420, 648
HphI GGTGA 2 cut(s) 219, 269
Hpy166II GTNNAC 6 cut(s) 153, 338, 712, 742, 762, 795
Hpy188I TCNGA 4 cut(s) 42, 134, 466, 826
Hpy188III TCNNGA 3 cut(s) 367, 652, 776
Hpy8I GTNNAC 6 cut(s) 153, 338, 712, 742, 762, 795
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 105
HpyCH4III ACNGT 4 cut(s) 157, 374, 562, 792
HpyCH4V TGCA 6 cut(s) 169, 281, 531, 581, 644, 787
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 9 cut(s) 31, 47, 107, 113, 119, 125, 131, 549, 798
Hsp92I GRCGYC 2 cut(s) 143, 703
Hsp92II CATG 3 cut(s) 535, 655, 689
HspAI GCGC 2 cut(s) 625, 703
KasI GGCGCC 1 cut(s) 702
Kzo9I GATC 3 cut(s) 346, 540, 805
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 2 cut(s) 403, 565
LweI GCATC 1 cut(s) 624
MaeI CTAG 3 cut(s) 198, 264, 776
MaeIII GTNAC 4 cut(s) 77, 225, 397, 436
MalI GATC 3 cut(s) 348, 542, 807
MboI GATC 3 cut(s) 346, 540, 805
MboII GAAGA 5 cut(s) 83, 345, 373, 590, 704
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 4 cut(s) 136, 460, 519, 836
Mly113I GGCGCC 1 cut(s) 703
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 7 cut(s) 36, 48, 114, 240, 693, 795, 820
MroXI GAANNNNTTC 1 cut(s) 720
MspA1I CMGCKG 2 cut(s) 320, 407
MspR9I CCNGG 2 cut(s) 333, 492
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 2 cut(s) 333, 492
MwoI GCNNNNNNNGC 1 cut(s) 287
NarI GGCGCC 1 cut(s) 703
NdeII GATC 3 cut(s) 346, 540, 805
NlaIII CATG 3 cut(s) 535, 655, 689
NlaIV GGNNCC 3 cut(s) 371, 683, 704
NmuCI GTSAC 3 cut(s) 225, 397, 436
NspI RCATGY 1 cut(s) 535
NspV TTCGAA 1 cut(s) 6
PagI TCATGA 1 cut(s) 651
PctI GAATGC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 3 cut(s) 185, 301, 648
Pfl23II CGTACG 1 cut(s) 177
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 3 cut(s) 71, 393, 580
PleI GAGTC 1 cut(s) 428
PluTI GGCGCC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 331, 490
PspEI GGTNACC 1 cut(s) 225
PspFI CCCAGC 3 cut(s) 316, 429, 605
PspGI CCWGG 2 cut(s) 331, 490
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 371, 683, 704
PspPI GGNCC 2 cut(s) 338, 682
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 179, 268, 502, 794
RsaNI GTAC 4 cut(s) 178, 267, 501, 793
SatI GCNGC 3 cut(s) 70, 392, 579
Sau3AI GATC 3 cut(s) 346, 540, 805
Sau96I GGNCC 2 cut(s) 338, 682
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 333, 492
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 1 cut(s) 624
SfoI GGCGCC 1 cut(s) 704
SfuI TTCGAA 1 cut(s) 6
SinI GGWCC 1 cut(s) 338
SpeI ACTAGT 2 cut(s) 197, 263
Sse9I AATT 4 cut(s) 136, 460, 519, 836
SsiI CCGC 2 cut(s) 70, 407
SspDI GGCGCC 1 cut(s) 702
SspI AATATT 1 cut(s) 872
SspMI CTAG 3 cut(s) 198, 264, 776
StyD4I CCNGG 2 cut(s) 331, 490
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 4 cut(s) 157, 374, 562, 792
TaqI TCGA 2 cut(s) 6, 140
TasI AATT 4 cut(s) 136, 460, 519, 836
TatI WGTACW 3 cut(s) 266, 500, 792
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 3 cut(s) 185, 301, 648
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 3 cut(s) 225, 397, 436
TseI GCWGC 2 cut(s) 391, 578
Tsp45I GTSAC 3 cut(s) 225, 397, 436
TspDTI ATGAA 3 cut(s) 45, 87, 373
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 802
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 3 cut(s) 136, 460, 836
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 3 cut(s) 198, 264, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.