pycom10g04050

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017372
Physical Location & Seq
Forward (+)
4333061 .. 4335565
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGGCTGCAACTGATCTAATGGTTAAGGTTAACTCGTCACAAGTTTGCCGCGATCATCCACCACCAAAACTGACCACCATAAAAAAGCTTATTGCGGAATCACAAGACACCAACGGCAACTTCATTGTTCCTCCCACTTATACCTACACAAACAATAATAATCCCCACGATGATCAGAAAGCTTTCGAAGAAGATACAGAAGCAGCAATCATTCCGACCGTTGACTTATCTCTTCTCGCAACATCTGCTACTCCTGAACAGCGCTCTAAAGCCATCAACGACATCGGCAAAGCCTGCCAGGAGTGGGGCTTCTTCATGGTGATCAATCATGGTGTGCCAGAGAGTCTGATGAAGGCAATCTTGGATGGTTGTGAGGGCTTTTTTGATCTGACGGAGGAGGAGAAGCAGGAGTTTGAAGGCAAGCACGTGTTGGACCCCATCAGATGCGGGACCAGCTTCAATGCCTCTGTGGAGAATGTTCGGTTTTGGAGGGATTTTCTCAAGGTTTTTGTATATCCTGATTTTCACTTCCCCTCTAAACCTGCTGGCTTCAGTGAGCTTGCATCAGAGTATTGCAAAAGAACAAGAGAAGTGACAAGGGAGCTACTGAAAGGAATATCAGAGAGCCTGGGGTTGGAGGCCACAGACTTTGACACGGCCTTGGATGTGGAATCAGCTGGCTTGCAAATCTTCATTGCAAACCTGTATCCTCCTTGTCCACAGCCAGAACTTGCAATGGGGATGCCTCCTCATTCTGATCATGGCCTCTTGACCCTTCTTATGCAGAATGGGATCAGTGGCCTTCAAGTGCAACACAAGGGTAAATGGGTCAACGTCAATGCTGCACCCTACTCCTTCCTTGTCAACACTGTTAGCAATGGAAAGTACAGGAGCAACATTCACAGGGCAGTGCTGAACAACAAATCAACAAGAATATCGGTAGCCACTCCGACTGGCCCATCACTTGAAACAGTAGTTAGACCTGCACCAAAGTTGGTAGACACTGCACAGCATCCGGCAGCAGCATATATTGGCATGAAGTACAAGGACTACTTGGAGCTTCAACAAGGCAACGTACTTGATGGGAAAGCCATCTTGGATCGTGTACGTGCGGGGGTTTCAAACTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

378

Amino Acids

41.63

Weight (kDa)

5.54

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 71 - 173 2e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 229 - 317 4.3e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017372)

Species Orthologous Gene IDs
malus_domestica MD10G1053500.v1.1
prunus_persica Prupe.8G066900_v2.0.a1
pyrus_communis pycom10g04050
rosa_chinensis RchiOBHm_Chr6g0271361
rosa_laevigata RLG00000013727
rosa_multiflora Rmu_sc0004866.1_g000028
rosa_roxburghii Rroxscaffold_7G00197070
rosa_rugosa Rorug06G0060500
rosa_samantha Rh6AG179600 Rh6BG182600 Rh6CG179500 Rh6DG171200
rosa_wichuraiana Rw6G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 550, 991
AccI GTMKAC 1 cut(s) 999
AccII CGCG 1 cut(s) 51
AciI CCGC 4 cut(s) 49, 95, 447, 1112
AclWI GGATC 2 cut(s) 800, 1107
AcuI CTGAAG 1 cut(s) 535
AcvI CACGTG 1 cut(s) 427
AfaI GTAC 4 cut(s) 887, 1043, 1077, 1107
AfeI AGCGCT 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 304, 634
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 6 cut(s) 416, 460, 806, 968, 1064, 1122
AjnI CCWGG 2 cut(s) 297, 627
AjuI GAANNNNNNNTTGG 6 cut(s) 104, 136, 344, 376, 1079, 1111
AluBI AGCT 7 cut(s) 88, 182, 456, 559, 604, 677, 1060
AluI AGCT 7 cut(s) 88, 182, 456, 559, 604, 677, 1060
AlwI GGATC 2 cut(s) 800, 1107
Aor51HI AGCGCT 1 cut(s) 263
AoxI GGCC 5 cut(s) 639, 657, 763, 799, 955
ApeKI GCWGC 5 cut(s) 5, 203, 842, 1019, 1022
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 3 cut(s) 433, 450, 956
AsuHPI GGTGA 1 cut(s) 331
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 2 cut(s) 433, 450
BbrPI CACGTG 1 cut(s) 427
BbvI GCAGC 4 cut(s) 215, 829, 1031, 1034
BccI CCATC 6 cut(s) 281, 359, 446, 967, 1076, 1100
BceAI ACGGC 2 cut(s) 130, 672
BciT130I CCWGG 2 cut(s) 299, 629
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 3 cut(s) 172, 321, 757
BfoI RGCGCY 1 cut(s) 265
BfuAI ACCTGC 2 cut(s) 550, 991
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 6 cut(s) 6, 49, 204, 843, 1020, 1023
BlsI GCNGC 6 cut(s) 7, 50, 205, 844, 1021, 1024
Bme1390I CCNGG 2 cut(s) 299, 629
Bme18I GGWCC 2 cut(s) 433, 450
BmgT120I GGNCC 3 cut(s) 433, 450, 956
BmiI GGNNCC 2 cut(s) 435, 451
BmrFI CCNGG 2 cut(s) 299, 629
BmsI GCATC 4 cut(s) 434, 572, 732, 1021
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 485
BsaAI YACGTR 2 cut(s) 427, 1109
BsaJI CCNNGG 2 cut(s) 628, 660
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 634
Bse1I ACTGG 1 cut(s) 958
Bse3DI GCAATG 3 cut(s) 693, 741, 883
BseBI CCWGG 2 cut(s) 299, 629
BseDI CCNNGG 2 cut(s) 628, 660
BseGI GGATG 5 cut(s) 55, 370, 670, 747, 1012
BseLI CCNNNNNNNGG 2 cut(s) 304, 634
BseMI GCAATG 3 cut(s) 693, 741, 883
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 3 cut(s) 410, 413, 738
BseXI GCAGC 4 cut(s) 215, 829, 1031, 1034
BsgI GTGCAG 3 cut(s) 828, 969, 990
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 641, 659, 765, 801, 957
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 1016
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 2 cut(s) 304, 634
BsmFI GGGAC 1 cut(s) 463
BsnI GGCC 5 cut(s) 641, 659, 765, 801, 957
Bsp119I TTCGAA 1 cut(s) 186
Bsp143I GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
BspACI CCGC 4 cut(s) 49, 95, 447, 1112
BspANI GGCC 5 cut(s) 641, 659, 765, 801, 957
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 435, 451
BspMI ACCTGC 2 cut(s) 550, 991
BspPI GGATC 2 cut(s) 800, 1107
BspT104I TTCGAA 1 cut(s) 186
BsrDI GCAATG 3 cut(s) 693, 741, 883
BsrI ACTGG 1 cut(s) 958
BssECI CCNNGG 2 cut(s) 628, 660
BssMI GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 299, 629
Bst4CI ACNGT 3 cut(s) 220, 871, 973
Bst6I CTCTTC 1 cut(s) 237
BstAPI GCANNNNNTGC 2 cut(s) 245, 294
BstBAI YACGTR 2 cut(s) 427, 1109
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 6 cut(s) 295, 422, 547, 561, 679, 683
BstF5I GGATG 5 cut(s) 55, 370, 670, 747, 1012
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 8 cut(s) 16, 55, 175, 324, 388, 760, 795, 1102
BstMBI GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 3 cut(s) 245, 294, 453
BstNI CCWGG 2 cut(s) 299, 629
BstSCI CCNGG 2 cut(s) 297, 627
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 4 cut(s) 215, 829, 1031, 1034
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 5 cut(s) 641, 659, 765, 801, 957
BtsCI GGATG 5 cut(s) 55, 370, 670, 747, 1012
BtsI GCAGTG 2 cut(s) 915, 1002
BtsIMutI CAGTG 5 cut(s) 559, 802, 867, 915, 1002
BveI ACCTGC 2 cut(s) 550, 991
Cac8I GCNNGC 6 cut(s) 295, 422, 547, 561, 679, 683
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 3 cut(s) 433, 450, 956
Csp6I GTAC 4 cut(s) 886, 1042, 1076, 1106
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 4 cut(s) 316, 329, 761, 1036
CviQI GTAC 4 cut(s) 886, 1042, 1076, 1106
DpnI GATC 8 cut(s) 15, 54, 174, 323, 387, 759, 794, 1101
DpnII GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 660
Eco47I GGWCC 2 cut(s) 433, 450
Eco47III AGCGCT 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 535
Eco72I CACGTG 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 297, 627
EcoT14I CCWWGG 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 4 cut(s) 319, 332, 764, 1039
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 1037, 1069
FaqI GGGAC 1 cut(s) 463
FatI CATG 4 cut(s) 315, 328, 760, 1035
FauI CCCGC 2 cut(s) 440, 1105
FbaI TGATCA 3 cut(s) 172, 321, 757
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 6 cut(s) 6, 49, 204, 843, 1020, 1023
FokI GGATG 5 cut(s) 42, 377, 677, 754, 999
Fsp4HI GCNGC 6 cut(s) 6, 49, 204, 843, 1020, 1023
GlaI GCGC 1 cut(s) 263
GluI GCNGC 6 cut(s) 6, 49, 204, 843, 1020, 1023
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 5 cut(s) 641, 659, 765, 801, 957
HapII CCGG 1 cut(s) 1016
HhaI GCGC 1 cut(s) 264
Hin1II CATG 4 cut(s) 319, 332, 764, 1039
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 4 cut(s) 31, 223, 832, 865
HindII GTYRAC 4 cut(s) 31, 223, 832, 865
HindIII AAGCTT 2 cut(s) 86, 180
HinfI GANTC 3 cut(s) 98, 343, 671
HpaI GTTAAC 1 cut(s) 31
HpaII CCGG 1 cut(s) 1016
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 7 cut(s) 31, 223, 719, 832, 865, 1000, 1106
Hpy188III TCNNGA 3 cut(s) 254, 518, 769
Hpy8I GTNNAC 7 cut(s) 31, 223, 719, 832, 865, 1000, 1106
HpyAV CCTTC 5 cut(s) 346, 410, 785, 812, 865
HpyCH4III ACNGT 3 cut(s) 220, 871, 973
HpyCH4IV ACGT 4 cut(s) 426, 834, 1074, 1108
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 294, 453
HpySE526I ACGT 4 cut(s) 426, 834, 1074, 1108
Hsp92II CATG 4 cut(s) 319, 332, 764, 1039
HspAI GCGC 1 cut(s) 262
Ksp22I TGATCA 3 cut(s) 172, 321, 757
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
LmnI GCTCC 3 cut(s) 601, 891, 1057
Lsp1109I GCAGC 4 cut(s) 215, 829, 1031, 1034
LweI GCATC 4 cut(s) 434, 572, 732, 1021
MaeII ACGT 4 cut(s) 426, 834, 1074, 1108
MaeIII GTNAC 2 cut(s) 36, 592
MalI GATC 8 cut(s) 15, 54, 174, 323, 387, 759, 794, 1101
MboI GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
MboII GAAGA 5 cut(s) 200, 203, 224, 304, 682
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 4 cut(s) 239, 411, 615, 974
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 2 cut(s) 24, 30
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 1 cut(s) 1016
MspR9I CCNGG 2 cut(s) 299, 629
MvaI CCWGG 2 cut(s) 299, 629
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 245, 294, 453
NdeII GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
NlaIII CATG 4 cut(s) 319, 332, 764, 1039
NlaIV GGNNCC 2 cut(s) 435, 451
NmuCI GTSAC 2 cut(s) 36, 592
NspV TTCGAA 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 98, 671
PkrI GCNGC 6 cut(s) 7, 50, 205, 844, 1021, 1024
PleI GAGTC 1 cut(s) 351
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
PpsI GAGTC 1 cut(s) 351
Ppu21I YACGTR 2 cut(s) 427, 1109
Psp6I CCWGG 2 cut(s) 297, 627
PspCI CACGTG 1 cut(s) 427
PspGI CCWGG 2 cut(s) 297, 627
PspN4I GGNNCC 2 cut(s) 435, 451
PspPI GGNCC 3 cut(s) 433, 450, 956
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 887, 1043, 1077, 1107
RsaNI GTAC 4 cut(s) 886, 1042, 1076, 1106
SaqAI TTAA 2 cut(s) 24, 30
SatI GCNGC 6 cut(s) 6, 49, 204, 843, 1020, 1023
Sau3AI GATC 8 cut(s) 13, 52, 172, 321, 385, 757, 792, 1099
Sau96I GGNCC 3 cut(s) 433, 450, 956
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 299, 629
SfaNI GCATC 4 cut(s) 434, 572, 732, 1021
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 2 cut(s) 433, 450
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SsiI CCGC 4 cut(s) 49, 95, 447, 1112
StyD4I CCNGG 2 cut(s) 297, 627
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 220, 871, 973
TaiI ACGT 4 cut(s) 429, 837, 1077, 1111
TaqI TCGA 1 cut(s) 186
TatI WGTACW 2 cut(s) 885, 1041
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 98, 671
Tru1I TTAA 2 cut(s) 24, 30
Tru9I TTAA 2 cut(s) 24, 30
TscAI CASTG 5 cut(s) 559, 802, 874, 915, 1009
TseFI GTSAC 2 cut(s) 36, 592
TseI GCWGC 5 cut(s) 5, 203, 842, 1019, 1022
Tsp45I GTSAC 2 cut(s) 36, 592
TspDTI ATGAA 5 cut(s) 112, 304, 365, 682, 1052
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 5 cut(s) 559, 802, 874, 915, 1009
VpaK11BI GGWCC 2 cut(s) 433, 450
XmiI GTMKAC 1 cut(s) 999
XmnI GAANNNNTTC 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.