pycom10g04470

Belongs to the glutamyl-tRNA reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003375
Physical Location & Seq
Reverse (-)
4685045 .. 4687577
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04470.1

Sequence Viewer

Length: 1629 bp
ATGGCGGCGGCTGCGAGTGGCTTCGCGGCCTCTCTTCCACGGCCCAGCGCGGAGCGCACCACCTCCTATTCTTTCTCTACATTGCATTCCCAAATCCGGATCCCGTACAGGCCTCTCCAAACCACAACGCTCAGAGGAGGACCCGCTTTGAACCCTAGGTGTGAAATGGGCTCCGAGAATTCGGTTCAGACGATCAACATCGGTTCCAGGTCTTTGAGCTCTTCGGCTCTCGAGCTCCTCAAAACCTCCGCAGCTGACAGATATACGAAAGAGAGGAGCAGCATTGCAGTAATAGGGCTGAATGTTCACACTGCTCCAGTTGAGATACGTGAGAAACTTGCCATCCCTGAAGCGCAGTGGTCTCAAGCCATTGGCGAGCTCTGTGCTTTGAACCATATAGAAGAAGCTGCTGTGCTTAGTACTTGTAACCGTATGGAAATATATGTTGTAGCTCTCTCTCAGCATCGTGGGGTTAAAGAAGTGACTGAATGGATGTCAAAGGTCAGTGGTGTTCCGGTCTCTGAGCTTTGTAAACATCGGGTTTTGCTGTACAACAAAGATGCCACCCAGCACCTATTTGAAATAGCTGCTGGGCTTGATTCTCTTGTGCTAGGAGAAGGTCAAATCCTTGCTCAAGTCAAACATGTTGTGAAAGTTGGACAGGGAGTACCTGGTTTTGATAGGAAAATCAGTGGTCTGTTCAAGCATGCAATCACTGTTGGGAAGCGAGTTAGATCGGAGACCAACATTGCCTCAGGGTCAGTTTCTGTCAGTTCAGCTGCTGTGGAGCTAGCCCTAATGAAGCTTCCAGAACCTTCTTATGCTACTGCCAGAGTGTTGGTGATTGGTGCAGGCAAGATGGGAAAACTTGTGATTAAACATTTAGTTGCAAAAGGGTGCACAAAAATGGTGATTGTTAATAGGAGTGAGGAGAGAGTGGCTGCCCTACGCGAAGAGTTAAAGGATGTTGAAATAATCTACAGGCCACTCTCAGATATGCTAGCATGCGCTGCTGAAGCGGATGTCATTTTCACTAGCACTGCATCAGAAGCCCCATTATTCTTGAAAGAACATGTACAGATGCTTCCCTCTGTGAGTCCAGAACTTGGAAATCGGCTTTTCATTGACATATCTGTCCCTAGGAATGTGGAACCATGCATCTCAGATCTTGAAGCTGCGACTGTATATAATGTAGATGACCTCAAAGAGGTTGTGGCTGCTCACAAGGAGGATAGGCTAAGGAAGGCTATGGAAGCTCAGACAATTATCACCGAGGAATCAAATAAATTTGAAGCTTGGAAGGATTCTCTTGAGACTGTTCCTACCATCAAGAAGTTTAGAGCTTATGCTGAGAGGATCAGGGCTTCCGAGGTTGAAAAGTGTTTGTCAAAGATGGGTGATGATATCTCTAAGAAGCAAAAAAAGGCTATTTACGATCTTAGCATGGGAATAGTGAACAAACTCCTTCATGGTCCAATGGAACACCTCAGATGTGATGGAAGTGACAATCGTGCTTTGAGTGAGATACTAGAGAATATGCGTGCTCTAAACAGAATGTTTGATCTCGAGACAGATACATCTTTGTTGGAAGAAAAGGTTCGAGCCAAGGTGGAACGAACCCGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
GlutR_N GlutR_dimer Shikimate_DH

Protein Analysis

543

Amino Acids

59.64

Weight (kDa)

8.42

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GlutR_N PF05201 98 - 248 5.1e-48 Glutamyl-tRNAGlu reductase, N-terminal domain
Shikimate_DH PF01488 264 - 404 5e-45 Shikimate / quinate 5-dehydrogenase
F420_oxidored PF03807 279 - 350 1.2e-07 NADP oxidoreductase coenzyme F420-dependent
GlutR_dimer PF00745 418 - 522 1.6e-26 Glutamyl-tRNAGlu reductase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1133
AccB7I CCANNNNNTGG 1 cut(s) 1106
AccII CGCG 3 cut(s) 26, 50, 951
AccIII TCCGGA 1 cut(s) 96
AciI CCGC 7 cut(s) 5, 8, 26, 50, 144, 249, 1019
AclWI GGATC 3 cut(s) 94, 107, 1364
AcsI RAATTY 2 cut(s) 178, 1286
AcuI CTGAAG 2 cut(s) 369, 1035
AfaI GTAC 5 cut(s) 107, 421, 551, 669, 1077
AfiI CCNNNNNNNGG 3 cut(s) 96, 1106, 1207
AflIII ACRYGT 2 cut(s) 643, 1072
AgsI TTSAA 9 cut(s) 151, 391, 581, 703, 971, 1066, 1172, 1292, 1376
AjnI CCWGG 2 cut(s) 206, 670
Alw21I GWGCWC 5 cut(s) 221, 237, 381, 902, 1546
Alw26I GTCTC 5 cut(s) 366, 523, 734, 1307, 1562
Alw44I GTGCAC 1 cut(s) 898
AlwI GGATC 3 cut(s) 94, 107, 1364
AlwNI CAGNNNCTG 2 cut(s) 767, 782
Ama87I CYCGRG 2 cut(s) 230, 1565
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 4 cut(s) 27, 41, 110, 983
ApaLI GTGCAC 1 cut(s) 898
ApoI RAATTY 2 cut(s) 178, 1286
Asp700I GAANNNNTTC 1 cut(s) 1596
AspA2I CCTAGG 2 cut(s) 155, 1139
AspLEI GCGC 4 cut(s) 50, 57, 355, 1010
AspS9I GGNCC 3 cut(s) 42, 140, 1472
AsuC2I CCSGG 1 cut(s) 1621
AsuHPI GGTGA 4 cut(s) 853, 922, 1261, 1409
AsuNHI GCTAGC 2 cut(s) 790, 1000
AvaI CYCGRG 2 cut(s) 230, 1565
AvaII GGWCC 2 cut(s) 140, 1472
AvrII CCTAGG 2 cut(s) 155, 1139
AxyI CCTNAGG 1 cut(s) 754
BaeGI GKGCMC 1 cut(s) 902
BamHI GGATCC 1 cut(s) 99
BanII GRGCYC 4 cut(s) 173, 221, 237, 381
BarI GAAGNNNNNNTAC 2 cut(s) 1068, 1100
Bbv12I GWGCWC 5 cut(s) 221, 237, 381, 902, 1546
BbvI GCAGC 9 cut(s) 263, 291, 394, 574, 766, 928, 997, 1162, 1204
BccI CCATC 5 cut(s) 350, 853, 1334, 1387, 1490
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 2 cut(s) 208, 672
BcnI CCSGG 1 cut(s) 1621
BcoDI GTCTC 5 cut(s) 366, 523, 734, 1307, 1562
BfaI CTAG 7 cut(s) 156, 611, 791, 1001, 1035, 1140, 1529
BfmI CTRYAG 1 cut(s) 979
BglII AGATCT 1 cut(s) 1165
BlnI CCTAGG 2 cut(s) 155, 1139
BmcAI AGTACT 1 cut(s) 421
Bme1390I CCNGG 3 cut(s) 208, 672, 1621
Bme18I GGWCC 2 cut(s) 140, 1472
BmeT110I CYCGRG 2 cut(s) 230, 1565
BmgT120I GGNCC 3 cut(s) 42, 140, 1472
BmiI GGNNCC 5 cut(s) 101, 142, 172, 205, 1152
BmrFI CCNGG 3 cut(s) 208, 672, 1621
BmsI GCATC 5 cut(s) 472, 550, 1052, 1071, 1167
BmtI GCTAGC 2 cut(s) 794, 1004
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 1238
BpuEI CTTGAG 3 cut(s) 348, 618, 1331
BpuMI CCSGG 1 cut(s) 1621
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 197
BsaI GGTCTC 3 cut(s) 366, 523, 734
BsaJI CCNNGG 6 cut(s) 38, 155, 1139, 1272, 1368, 1605
BsaWI WCCGGW 2 cut(s) 96, 514
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 1106, 1207
Bse1I ACTGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 754
Bse3DI GCAATG 3 cut(s) 80, 282, 747
Bse8I GATNNNNATC 1 cut(s) 197
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 2 cut(s) 208, 672
BseDI CCNNGG 6 cut(s) 38, 155, 1139, 1272, 1368, 1605
BseGI GGATG 4 cut(s) 342, 498, 970, 1027
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 3 cut(s) 96, 1106, 1207
BseMI GCAATG 3 cut(s) 80, 282, 747
BseMII CTCAG 9 cut(s) 145, 473, 513, 768, 1005, 1176, 1271, 1341, 1501
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 4 cut(s) 150, 227, 289, 944
BseSI GKGCMC 1 cut(s) 902
BseXI GCAGC 9 cut(s) 263, 291, 394, 574, 766, 928, 997, 1162, 1204
BseYI CCCAGC 3 cut(s) 44, 567, 590
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 3 cut(s) 26, 50, 951
BshFI GGCC 4 cut(s) 29, 43, 112, 985
BsiHKAI GWGCWC 5 cut(s) 221, 237, 381, 902, 1546
BsiHKCI CYCGRG 2 cut(s) 230, 1565
BsiSI CCGG 3 cut(s) 97, 515, 1621
BslFI GGGAC 1 cut(s) 1121
BslI CCNNNNNNNGG 3 cut(s) 96, 1106, 1207
BsmAI GTCTC 5 cut(s) 366, 523, 734, 1307, 1562
BsmFI GGGAC 1 cut(s) 1121
BsmI GAATGC 1 cut(s) 85
BsnI GGCC 4 cut(s) 29, 43, 112, 985
Bso31I GGTCTC 3 cut(s) 366, 523, 734
BsoBI CYCGRG 2 cut(s) 230, 1565
Bsp1286I GDGCHC 6 cut(s) 173, 221, 237, 381, 902, 1546
Bsp13I TCCGGA 1 cut(s) 96
Bsp1407I TGTACA 2 cut(s) 549, 1075
Bsp143I GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
BspACI CCGC 7 cut(s) 5, 8, 26, 50, 144, 249, 1019
BspANI GGCC 4 cut(s) 29, 43, 112, 985
BspCNI CTCAG 9 cut(s) 144, 472, 514, 767, 1004, 1175, 1270, 1342, 1500
BspEI TCCGGA 1 cut(s) 96
BspFNI CGCG 3 cut(s) 26, 50, 951
BspLI GGNNCC 5 cut(s) 101, 142, 172, 205, 1152
BspOI GCTAGC 2 cut(s) 794, 1004
BspPI GGATC 3 cut(s) 94, 107, 1364
BspQI GCTCTTC 1 cut(s) 226
BspTNI GGTCTC 3 cut(s) 366, 523, 734
BsrDI GCAATG 3 cut(s) 80, 282, 747
BsrGI TGTACA 2 cut(s) 549, 1075
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 6 cut(s) 38, 155, 1139, 1272, 1368, 1605
BssMI GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
BssT1I CCWWGG 3 cut(s) 155, 1139, 1605
Bst2UI CCWGG 2 cut(s) 208, 672
Bst4CI ACNGT 4 cut(s) 431, 718, 1183, 1318
Bst6I CTCTTC 3 cut(s) 39, 226, 948
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstAUI TGTACA 2 cut(s) 549, 1075
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 7 cut(s) 377, 708, 792, 853, 1002, 1006, 1542
BstDSI CCRYGG 1 cut(s) 38
BstENI CCTNNNNNAGG 1 cut(s) 1205
BstF5I GGATG 4 cut(s) 342, 498, 970, 1027
BstFNI CGCG 3 cut(s) 26, 50, 951
BstHHI GCGC 4 cut(s) 50, 57, 355, 1010
BstKTI GATC 7 cut(s) 102, 195, 737, 1168, 1359, 1438, 1564
BstMAI GTCTC 5 cut(s) 366, 523, 734, 1307, 1562
BstMBI GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
BstMWI GCNNNNNNNGC 6 cut(s) 11, 54, 1010, 1016, 1049, 1253
BstNI CCWGG 2 cut(s) 208, 672
BstNSI RCATGY 4 cut(s) 647, 710, 1008, 1076
BstSCI CCNGG 3 cut(s) 206, 670, 1619
BstSFI CTRYAG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 902
BstUI CGCG 3 cut(s) 26, 50, 951
BstV1I GCAGC 9 cut(s) 263, 291, 394, 574, 766, 928, 997, 1162, 1204
BstX2I RGATCY 2 cut(s) 99, 1165
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 2 cut(s) 99, 1165
Bsu36I CCTNAGG 1 cut(s) 754
BsuRI GGCC 4 cut(s) 29, 43, 112, 985
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 342, 498, 970, 1027
BtsI GCAGTG 3 cut(s) 309, 362, 1038
BtsIMutI CAGTG 6 cut(s) 309, 362, 511, 697, 714, 1038
Cac8I GCNNGC 7 cut(s) 377, 708, 792, 853, 1002, 1006, 1542
CaiI CAGNNNCTG 2 cut(s) 767, 782
CfoI GCGC 4 cut(s) 50, 57, 355, 1010
Cfr13I GGNCC 3 cut(s) 42, 140, 1472
CsiI ACCWGGT 1 cut(s) 670
Csp6I GTAC 5 cut(s) 106, 420, 550, 668, 1076
CviAII CATG 7 cut(s) 644, 707, 1005, 1073, 1155, 1444, 1469
CviQI GTAC 5 cut(s) 106, 420, 550, 668, 1076
DpnI GATC 7 cut(s) 101, 194, 736, 1167, 1358, 1437, 1563
DpnII GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
DrdI GACNNNNNNGTC 1 cut(s) 1133
DseDI GACNNNNNNGTC 1 cut(s) 1133
Eam1104I CTCTTC 3 cut(s) 39, 226, 948
EarI CTCTTC 3 cut(s) 39, 226, 948
Ecl136II GAGCTC 3 cut(s) 219, 235, 379
Eco130I CCWWGG 3 cut(s) 155, 1139, 1605
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 4 cut(s) 173, 221, 237, 381
Eco31I GGTCTC 3 cut(s) 366, 523, 734
Eco32I GATATC 1 cut(s) 1405
Eco47I GGWCC 2 cut(s) 140, 1472
Eco53kI GAGCTC 3 cut(s) 219, 235, 379
Eco57I CTGAAG 2 cut(s) 369, 1035
Eco81I CCTNAGG 1 cut(s) 754
Eco88I CYCGRG 2 cut(s) 230, 1565
EcoICRI GAGCTC 3 cut(s) 219, 235, 379
EcoNI CCTNNNNNAGG 1 cut(s) 1205
EcoO109I RGGNCCY 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 206, 670
EcoRV GATATC 1 cut(s) 1405
EcoT14I CCWWGG 3 cut(s) 155, 1139, 1605
EcoT22I ATGCAT 1 cut(s) 1160
EcoT38I GRGCYC 4 cut(s) 173, 221, 237, 381
ErhI CCWWGG 3 cut(s) 155, 1139, 1605
FaeI CATG 7 cut(s) 647, 710, 1008, 1076, 1158, 1447, 1472
FaqI GGGAC 1 cut(s) 1121
FatI CATG 7 cut(s) 643, 706, 1004, 1072, 1154, 1443, 1468
FauI CCCGC 1 cut(s) 151
FokI GGATG 4 cut(s) 329, 505, 977, 1034
FriOI GRGCYC 4 cut(s) 173, 221, 237, 381
FspBI CTAG 7 cut(s) 156, 611, 791, 1001, 1035, 1140, 1529
GlaI GCGC 4 cut(s) 49, 56, 354, 1009
GsaI CCCAGC 3 cut(s) 48, 571, 594
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 4 cut(s) 29, 43, 112, 985
HapII CCGG 3 cut(s) 97, 515, 1621
HhaI GCGC 4 cut(s) 50, 57, 355, 1010
Hin1II CATG 7 cut(s) 647, 710, 1008, 1076, 1158, 1447, 1472
Hin6I GCGC 4 cut(s) 48, 55, 353, 1008
HinP1I GCGC 4 cut(s) 48, 55, 353, 1008
HindIII AAGCTT 2 cut(s) 803, 1293
HinfI GANTC 4 cut(s) 599, 1096, 1277, 1304
HpaII CCGG 3 cut(s) 97, 515, 1621
HphI GGTGA 4 cut(s) 853, 922, 1261, 1409
Hpy166II GTNNAC 4 cut(s) 307, 533, 900, 1456
Hpy8I GTNNAC 4 cut(s) 307, 533, 900, 1456
HpyAV CCTTC 5 cut(s) 611, 825, 1237, 1294, 1475
HpyCH4III ACNGT 4 cut(s) 431, 718, 1183, 1318
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 8 cut(s) 85, 287, 710, 851, 890, 900, 1043, 1158
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 54, 1010, 1016, 1049, 1253
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 7 cut(s) 647, 710, 1008, 1076, 1158, 1447, 1472
HspAI GCGC 4 cut(s) 48, 55, 353, 1008
Kpn2I TCCGGA 1 cut(s) 96
Kzo9I GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
LguI GCTCTTC 1 cut(s) 226
LmnI GCTCC 6 cut(s) 52, 176, 240, 276, 319, 787
Lsp1109I GCAGC 9 cut(s) 263, 291, 394, 574, 766, 928, 997, 1162, 1204
LweI GCATC 5 cut(s) 472, 550, 1052, 1071, 1167
MabI ACCWGGT 1 cut(s) 670
MaeI CTAG 7 cut(s) 156, 611, 791, 1001, 1035, 1140, 1529
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 3 cut(s) 425, 481, 1502
MalI GATC 7 cut(s) 101, 194, 736, 1167, 1358, 1437, 1563
MboI GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
MboII GAAGA 5 cut(s) 26, 213, 413, 965, 1601
MflI RGATCY 2 cut(s) 99, 1165
MhlI GDGCHC 6 cut(s) 173, 221, 237, 381, 902, 1546
MluCI AATT 3 cut(s) 178, 1263, 1286
MlyI GAGTC 1 cut(s) 1105
MmeI TCCRAC 2 cut(s) 637, 1566
Mph1103I ATGCAT 1 cut(s) 1160
MroI TCCGGA 1 cut(s) 96
MroXI GAANNNNTTC 1 cut(s) 1596
MseI TTAA 4 cut(s) 474, 876, 918, 959
MslI CAYNNNNRTG 1 cut(s) 905
MspA1I CMGCKG 2 cut(s) 254, 779
MspI CCGG 3 cut(s) 97, 515, 1621
MspR9I CCNGG 3 cut(s) 208, 672, 1621
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 2 cut(s) 208, 672
MvnI CGCG 3 cut(s) 26, 50, 951
MwoI GCNNNNNNNGC 6 cut(s) 11, 54, 1010, 1016, 1049, 1253
NciI CCSGG 1 cut(s) 1621
NdeII GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
NheI GCTAGC 2 cut(s) 790, 1000
NlaIII CATG 7 cut(s) 647, 710, 1008, 1076, 1158, 1447, 1472
NlaIV GGNNCC 5 cut(s) 101, 142, 172, 205, 1152
NmuCI GTSAC 2 cut(s) 481, 1502
NsiI ATGCAT 1 cut(s) 1160
NspI RCATGY 4 cut(s) 647, 710, 1008, 1076
PaeI GCATGC 2 cut(s) 710, 1008
PaeR7I CTCGAG 2 cut(s) 230, 1565
PceI AGGCCT 1 cut(s) 112
PciI ACATGT 2 cut(s) 643, 1072
PciSI GCTCTTC 1 cut(s) 226
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 1596
PfeI GAWTC 3 cut(s) 599, 1277, 1304
PflMI CCANNNNNTGG 1 cut(s) 1106
PleI GAGTC 1 cut(s) 1104
PpsI GAGTC 1 cut(s) 1104
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 140
PscI ACATGT 2 cut(s) 643, 1072
Psp124BI GAGCTC 3 cut(s) 221, 237, 381
Psp5II RGGWCCY 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 206, 670
PspFI CCCAGC 3 cut(s) 44, 567, 590
PspGI CCWGG 2 cut(s) 206, 670
PspN4I GGNNCC 5 cut(s) 101, 142, 172, 205, 1152
PspPI GGNCC 3 cut(s) 42, 140, 1472
PspPPI RGGWCCY 1 cut(s) 140
PstNI CAGNNNCTG 2 cut(s) 767, 782
PsuI RGATCY 2 cut(s) 99, 1165
PvuII CAGCTG 2 cut(s) 254, 779
RsaI GTAC 5 cut(s) 107, 421, 551, 669, 1077
RsaNI GTAC 5 cut(s) 106, 420, 550, 668, 1076
RseI CAYNNNNRTG 1 cut(s) 905
SacI GAGCTC 3 cut(s) 221, 237, 381
SapI GCTCTTC 1 cut(s) 226
SaqAI TTAA 4 cut(s) 474, 876, 918, 959
Sau3AI GATC 7 cut(s) 99, 192, 734, 1165, 1356, 1435, 1561
Sau96I GGNCC 3 cut(s) 42, 140, 1472
ScaI AGTACT 1 cut(s) 421
SchI GAGTC 1 cut(s) 1105
ScrFI CCNGG 3 cut(s) 208, 672, 1621
SduI GDGCHC 6 cut(s) 173, 221, 237, 381, 902, 1546
SexAI ACCWGGT 1 cut(s) 670
SfaNI GCATC 5 cut(s) 472, 550, 1052, 1071, 1167
SfcI CTRYAG 1 cut(s) 979
Sfr274I CTCGAG 2 cut(s) 230, 1565
SinI GGWCC 2 cut(s) 140, 1472
SlaI CTCGAG 2 cut(s) 230, 1565
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 5 cut(s) 230, 363, 633, 1310, 1565
SmoI CTYRAG 5 cut(s) 230, 363, 633, 1310, 1565
SphI GCATGC 2 cut(s) 710, 1008
Sse9I AATT 3 cut(s) 178, 1263, 1286
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 7 cut(s) 5, 8, 26, 50, 144, 249, 1019
SspMI CTAG 7 cut(s) 156, 611, 791, 1001, 1035, 1140, 1529
SstI GAGCTC 3 cut(s) 221, 237, 381
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 3 cut(s) 206, 670, 1619
StyI CCWWGG 3 cut(s) 155, 1139, 1605
TaaI ACNGT 4 cut(s) 431, 718, 1183, 1318
TaiI ACGT 1 cut(s) 331
TaqI TCGA 3 cut(s) 231, 1566, 1600
TasI AATT 3 cut(s) 178, 1263, 1286
TatI WGTACW 3 cut(s) 419, 549, 1075
TauI GCSGC 3 cut(s) 8, 11, 29
TfiI GAWTC 3 cut(s) 599, 1277, 1304
Tru1I TTAA 4 cut(s) 474, 876, 918, 959
Tru9I TTAA 4 cut(s) 474, 876, 918, 959
TscAI CASTG 6 cut(s) 316, 362, 511, 697, 721, 1045
TseFI GTSAC 2 cut(s) 481, 1502
Tsp45I GTSAC 2 cut(s) 481, 1502
TspDTI ATGAA 3 cut(s) 815, 1111, 1457
TspRI CASTG 6 cut(s) 316, 362, 511, 697, 721, 1045
Van91I CCANNNNNTGG 1 cut(s) 1106
VneI GTGCAC 1 cut(s) 898
VpaK11BI GGWCC 2 cut(s) 140, 1472
XagI CCTNNNNNAGG 1 cut(s) 1205
XapI RAATTY 2 cut(s) 178, 1286
XceI RCATGY 4 cut(s) 647, 710, 1008, 1076
XhoI CTCGAG 2 cut(s) 230, 1565
XmaJI CCTAGG 2 cut(s) 155, 1139
XmnI GAANNNNTTC 1 cut(s) 1596
XspI CTAG 7 cut(s) 156, 611, 791, 1001, 1035, 1140, 1529
ZrmI AGTACT 1 cut(s) 421
Zsp2I ATGCAT 1 cut(s) 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.