pycom10g10190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010959
Physical Location & Seq
Forward (+)
13478210 .. 13478599
390 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
ATGGCTTATTATGGAAGAAGCCCAACCAGCTCCTTCTTAGAAGGGTTTTCTCTATGTCCTCTGCCCTACCCAGTTCTACTAATCCTAGCAGTACTCTCTATCTTTTTAGGCATCTCATGGTACTCCTCCATTGAGTCAGCTGTGGAAGAAGTTGGACTGCAATTCAATTGGGTGTTTTTGCTCACACCAGTAGTTCTCATCTTCATTGTGAAGGGGCTCTCATATATGGATCCTGATTGGCTCTTCCCCATGTCGCTGTGGGAACGACGCCGGAGGACTCACCATATACCTTCAGAAGGAAGCTCACCTTGGGGTGTTGCTGCTTTCATTGTGGTCTTACTTGTTTTGCTGAATTACCAGTCAATTTTTCGTGATAGCTGGCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.82

Weight (kDa)

5.53

Isoelectric Point (pI)

72.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 224, 237
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 2 cut(s) 93, 122
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 292, 324
AluBI AGCT 4 cut(s) 30, 140, 303, 378
AluI AGCT 4 cut(s) 30, 140, 303, 378
AlwI GGATC 2 cut(s) 224, 237
ApeKI GCWGC 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 272, 297
BamHI GGATCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 307
BfaI CTAG 1 cut(s) 86
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
BmcAI AGTACT 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 231
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 71, 188, 358
BseDI CCNNGG 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 296
BseNI ACTGG 3 cut(s) 71, 188, 358
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 2 cut(s) 224, 237
BspQI GCTCTTC 1 cut(s) 248
BsrI ACTGG 3 cut(s) 71, 188, 358
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 1 cut(s) 229
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 380
BstDEI CTNAG 1 cut(s) 37
BstENI CCTNNNNNAGG 1 cut(s) 294
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 380
CseI GACGC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 92, 121
CviAII CATG 2 cut(s) 117, 250
CviJI RGCY 9 cut(s) 5, 21, 30, 140, 217, 241, 303, 378, 382
CviKI_1 RGCY 9 cut(s) 5, 21, 30, 140, 217, 241, 303, 378, 382
CviQI GTAC 2 cut(s) 92, 121
DdeI CTNAG 1 cut(s) 37
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 2 cut(s) 120, 253
FaiI YATR 9 cut(s) 12, 55, 118, 223, 225, 227, 251, 285, 287
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FatI CATG 2 cut(s) 116, 249
Fnu4HI GCNGC 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 1 cut(s) 86
GluI GCNGC 1 cut(s) 321
HapII CCGG 1 cut(s) 271
HgaI GACGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 2 cut(s) 120, 253
HinfI GANTC 2 cut(s) 134, 277
HpaII CCGG 1 cut(s) 271
HphI GGTGA 2 cut(s) 272, 297
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 2 cut(s) 233, 371
Hpy99I CGWCG 1 cut(s) 270
HpyAV CCTTC 5 cut(s) 35, 43, 205, 290, 300
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpyF3I CTNAG 1 cut(s) 37
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 2 cut(s) 120, 253
Kzo9I GATC 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 1 cut(s) 35
LpnPI CCDG 7 cut(s) 40, 84, 201, 246, 284, 364, 371
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 86
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 27, 158, 193, 235
MfeI CAATTG 1 cut(s) 166
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 4 cut(s) 161, 166, 352, 363
MlyI GAGTC 2 cut(s) 143, 271
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 69, 136, 267
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 27
NdeII GATC 1 cut(s) 229
NlaIII CATG 2 cut(s) 120, 253
NlaIV GGNNCC 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 2 cut(s) 142, 271
PpsI GAGTC 2 cut(s) 142, 271
PspN4I GGNNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 2 cut(s) 93, 122
RsaNI GTAC 2 cut(s) 92, 121
SapI GCTCTTC 1 cut(s) 248
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 229
ScaI AGTACT 1 cut(s) 93
SchI GAGTC 2 cut(s) 143, 271
SduI GDGCHC 1 cut(s) 219
SetI ASST 6 cut(s) 32, 142, 292, 305, 310, 380
SfaNI GCATC 1 cut(s) 120
Sse9I AATT 4 cut(s) 161, 166, 352, 363
SspMI CTAG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 308
TasI AATT 4 cut(s) 161, 166, 352, 363
TatI WGTACW 1 cut(s) 91
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 193, 316
XagI CCTNNNNNAGG 1 cut(s) 294
XspI CTAG 1 cut(s) 86
ZrmI AGTACT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.