pycom10g10670

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017042
Physical Location & Seq
Forward (+)
14057168 .. 14058244
1077 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGGCCTCAAAGAGAATATTGAAGGAGCTCAAGGACTTGCAAAGAGATCCACCAACTTCATGTAGTGCAGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCAACAATAATTGGTCCAAATGATAGCCCCTATTCTGGGGGTGTCTTCCTTGTGACTATCAATTTTCCACCTGATTATCCTTTCAAACCTCCTAAGGTTGCCTTCAAAACCAAAGTTTTCCATCCAAATATAAACAGTAACGGCAATATTTGCTTGGACATTCTCAAGGAGCAATGGAGTCCAGCACTCACCATATCGAAGGTTTTACTGTCCATATGTTCATTGCTGACGGATCCAAACCCTGATGACCCTCTTGTACCTGAGATTGCTCATATGTGCAAGTGTGACAAAGGCAAATATGAGTCAACGGCTCGGAGCTGGACCCAGAAGTACGCCATGGATTAA

Protein Analysis

149

Amino Acids

16.49

Weight (kDa)

6.81

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 1.9e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16740
fragaria_vesca FvH4_2g01240
malus_domestica MD05G1121200.v1.1
prunus_persica Prupe.8G165100_v2.0.a1
pyrus_communis pycom05g11540 pycom10g10670
rosa_chinensis RchiOBHm_Chr6g0251901
rosa_laevigata RLG00000015421
rosa_multiflora Rmu_ssc0000303.1_g000012
rosa_roxburghii Rroxscaffold_7G00216320
rosa_rugosa Rorug05G0504900
rosa_samantha Rh6BG014100
rosa_wichuraiana Rw6G001540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AclWI GGATC 3 cut(s) 41, 329, 342
AfaI GTAC 2 cut(s) 360, 434
AfiI CCNNNNNNNGG 2 cut(s) 137, 138
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 3 cut(s) 22, 187, 208
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw21I GWGCWC 1 cut(s) 30
AlwI GGATC 3 cut(s) 41, 329, 342
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 3
AspS9I GGNCC 3 cut(s) 71, 116, 423
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 3 cut(s) 71, 116, 423
AxyI CCTNAGG 1 cut(s) 195
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 81
BccI CCATC 1 cut(s) 231
BceAI ACGGC 2 cut(s) 259, 426
BfuAI ACCTGC 1 cut(s) 59
Bme18I GGWCC 3 cut(s) 71, 116, 423
BmgT120I GGNCC 3 cut(s) 71, 116, 423
BmiI GGNNCC 2 cut(s) 336, 425
BpiI GAAGAC 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 14, 251
BsaJI CCNNGG 1 cut(s) 438
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 138
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 2 cut(s) 281, 323
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 137, 138
BseMI GCAATG 2 cut(s) 281, 323
BseMII CTCAG 2 cut(s) 72, 354
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 137, 138
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 46, 334
Bsp19I CCATGG 1 cut(s) 438
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 73, 355
BspLI GGNNCC 2 cut(s) 336, 425
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 3 cut(s) 41, 329, 342
BsrDI GCAATG 2 cut(s) 281, 323
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 2 cut(s) 46, 334
BssT1I CCWWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 239, 312
BstAPI GCANNNNNTGC 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 3 cut(s) 81, 195, 363
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 2 cut(s) 49, 337
BstMBI GATC 2 cut(s) 46, 334
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 91
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 2 cut(s) 46, 334
BstYI RGATCY 2 cut(s) 46, 334
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 71, 116, 423
Csp6I GTAC 2 cut(s) 359, 433
CviAII CATG 3 cut(s) 60, 88, 439
CviJI RGCY 6 cut(s) 5, 28, 80, 129, 413, 420
CviKI_1 RGCY 6 cut(s) 5, 28, 80, 129, 413, 420
CviQI GTAC 2 cut(s) 359, 433
DdeI CTNAG 3 cut(s) 81, 195, 363
DpnI GATC 2 cut(s) 48, 336
DpnII GATC 2 cut(s) 46, 334
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 3 cut(s) 71, 116, 423
Eco53kI GAGCTC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 3 cut(s) 63, 91, 442
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FatI CATG 3 cut(s) 59, 87, 438
FauNDI CATATG 2 cut(s) 317, 375
FokI GGATG 1 cut(s) 210
FriOI GRGCYC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 3 cut(s) 63, 91, 442
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 280, 404
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 1 cut(s) 417
Hpy8I GTNNAC 1 cut(s) 408
HpyAV CCTTC 3 cut(s) 16, 214, 295
HpyCH4III ACNGT 2 cut(s) 239, 312
HpyCH4V TGCA 3 cut(s) 40, 68, 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 3 cut(s) 81, 195, 363
Hsp92II CATG 3 cut(s) 63, 91, 442
Kzo9I GATC 2 cut(s) 46, 334
LmnI GCTCC 3 cut(s) 25, 271, 417
LpnPI CCDG 9 cut(s) 54, 87, 123, 186, 297, 357, 375, 406, 440
MaeIII GTNAC 3 cut(s) 154, 239, 386
MalI GATC 2 cut(s) 48, 336
MboI GATC 2 cut(s) 46, 334
MboII GAAGA 1 cut(s) 139
MflI RGATCY 2 cut(s) 46, 334
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 111, 163
MlyI GAGTC 2 cut(s) 289, 413
MnlI CCTC 4 cut(s) 16, 76, 201, 363
MseI TTAA 1 cut(s) 445
MwoI GCNNNNNNNGC 1 cut(s) 252
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 2 cut(s) 317, 375
NdeII GATC 2 cut(s) 46, 334
NlaIII CATG 3 cut(s) 63, 91, 442
NlaIV GGNNCC 2 cut(s) 336, 425
NmuCI GTSAC 2 cut(s) 154, 386
NspI RCATGY 1 cut(s) 91
PciI ACATGT 1 cut(s) 87
PleI GAGTC 2 cut(s) 288, 412
PpsI GAGTC 2 cut(s) 288, 412
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 336, 425
PspPI GGNCC 3 cut(s) 71, 116, 423
PspPPI RGGWCCY 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 2 cut(s) 46, 334
RsaI GTAC 2 cut(s) 360, 434
RsaNI GTAC 2 cut(s) 359, 433
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 445
Sau3AI GATC 2 cut(s) 46, 334
Sau96I GGNCC 3 cut(s) 71, 116, 423
SchI GAGTC 2 cut(s) 289, 413
SduI GDGCHC 1 cut(s) 30
SetI ASST 8 cut(s) 30, 73, 175, 193, 201, 306, 364, 422
SinI GGWCC 3 cut(s) 71, 116, 423
SmlI CTYRAG 2 cut(s) 29, 266
SmoI CTYRAG 2 cut(s) 29, 266
Sse9I AATT 2 cut(s) 111, 163
SspI AATATT 2 cut(s) 18, 250
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 239, 312
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 111, 163
Tru1I TTAA 1 cut(s) 445
Tru9I TTAA 1 cut(s) 445
TseFI GTSAC 2 cut(s) 154, 386
Tsp45I GTSAC 2 cut(s) 154, 386
TspDTI ATGAA 3 cut(s) 48, 83, 312
TspGWI ACGGA 1 cut(s) 347
VpaK11BI GGWCC 3 cut(s) 71, 116, 423
XceI RCATGY 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.