pycom10g12870

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017243
Physical Location & Seq
Forward (+)
16351538 .. 16352885
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGCCGGCTTCCATAAGCATGGATGAGAATCTGGGGTTCCAGTATGGAGTTTCCAGGCCAGGGCTTAATCCGCCAATTCAATTTGGGAATGGATTTTGTGGAGCAGGGTCAGGACTCATTCGAGGCGGATTGGGTGCGTATGGAAAGAAAATTTGTGGTTCAAGTTCTCAGTATGTTCAAAGCAATATAAGCAAGTACTTCTCAGATCCTCAATACTACTTCCAAGTGAACTCCCACTACGTCCGAAACAAACTGAAGATAATTCTGTTTCCATTTCTGCACAGGGGGCACTGGACGAGGATGACAGAGCCAGTTGGAGGCAGGCTTTCGTATAACCCTCCAATTACTGACATACATGCCCCAGACTTGTACATTCCATTCATGACATTTGCAACGTACTTGGTTCTCGCTGGGATCTCACTAGGTCTTTCTGGGAACCCAGAGGCTATAAATTGGCAGTTTGTGAAGGGAATGGTGGGTTGGCTACTCCAAGTGATGCTGCTCAAGGCATCGTTGTCTTCACTTGGGGGTGGAGAGGCACCTTTGCTAGACATGATAGCATATACTGGGTACACTTTCACAGGGTTGTGTGTGGCGGTTCTAGGGCGAATCACGCTAAGCTATGCGTACTACTTGATAATCATATGGACATCCATGTGCTCAGGCATCTTCTTGGCAAAGACAATTAAGATAACTTTGTATGCTGAGATCAACAGTTATGATGCAAACATGCATCATTATATGTTGCTAGGTATCGCGTTTTCTCAGTTCCCGTTGATCATCTGGCTGAGCAACCCTACCGGGAATTGGTTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

273

Amino Acids

30.07

Weight (kDa)

8.94

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 46 - 264 1.1e-48 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017243)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17570
malus_domestica MD10G1149100.v1.1
prunus_persica Prupe.8G187900_v2.0.a1
pyrus_communis pycom10g12870
rosa_chinensis RchiOBHm_Chr6g0282511
rosa_multiflora Rmu_sc0000283.1_g000013 Rmu_sc0005697.1_g000030
rosa_roxburghii Rroxscaffold_7G00186300
rosa_rugosa Rorug06G0147000
rosa_samantha Rh6AG260400 Rh6CG262600 Rh6DG254500
rosa_wichuraiana Rw6G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccII CGCG 1 cut(s) 758
AciI CCGC 3 cut(s) 71, 126, 596
AclWI GGATC 2 cut(s) 200, 422
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 5 cut(s) 197, 371, 398, 572, 629
AfiI CCNNNNNNNGG 3 cut(s) 60, 317, 807
AgsI TTSAA 3 cut(s) 80, 162, 179
AjnI CCWGG 2 cut(s) 53, 58
AjuI GAANNNNNNNTTGG 2 cut(s) 463, 495
AluBI AGCT 1 cut(s) 621
AluI AGCT 1 cut(s) 621
Alw21I GWGCWC 1 cut(s) 662
AlwI GGATC 2 cut(s) 200, 422
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 499
ApoI RAATTY 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 802
BaeGI GKGCMC 1 cut(s) 291
BanI GGYRCC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 662
BbvI GCAGC 1 cut(s) 486
BciT130I CCWGG 2 cut(s) 55, 60
BclI TGATCA 1 cut(s) 777
BcnI CCSGG 1 cut(s) 802
BfaI CTAG 4 cut(s) 422, 548, 602, 749
BisI GCNGC 1 cut(s) 500
BlpI GCTNAGC 2 cut(s) 617, 788
BlsI GCNGC 1 cut(s) 501
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 3 cut(s) 55, 60, 802
BmiI GGNNCC 3 cut(s) 38, 437, 540
BmrFI CCNGG 3 cut(s) 55, 60, 802
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 5 cut(s) 486, 518, 675, 712, 742
BmuI ACTGGG 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 661
Bpu1102I GCTNAGC 2 cut(s) 617, 788
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 802
BsaBI GATNNNNATC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 317, 807
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 4 cut(s) 40, 296, 311, 571
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 2 cut(s) 55, 60
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 3 cut(s) 28, 306, 650
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 60, 317, 807
BseMII CTCAG 6 cut(s) 182, 216, 675, 696, 779, 779
BseNI ACTGG 4 cut(s) 40, 296, 311, 571
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 1 cut(s) 486
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 758
BshFI GGCC 1 cut(s) 58
BshNI GGYRCC 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 662
BsiSI CCGG 2 cut(s) 5, 801
BslI CCNNNNNNNGG 3 cut(s) 60, 317, 807
BsnI GGCC 1 cut(s) 58
Bsp1286I GDGCHC 2 cut(s) 291, 662
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 205, 414, 708, 777
Bsp1720I GCTNAGC 2 cut(s) 617, 788
BspACI CCGC 3 cut(s) 71, 126, 596
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 6 cut(s) 181, 215, 674, 697, 778, 780
BspFNI CGCG 1 cut(s) 758
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 3 cut(s) 38, 437, 540
BspPI GGATC 2 cut(s) 200, 422
BspT107I GGYRCC 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 40, 296, 311, 571
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 4 cut(s) 205, 414, 708, 777
Bst2UI CCWGG 2 cut(s) 55, 60
Bst4CI ACNGT 1 cut(s) 716
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 6, 323
BstDEI CTNAG 7 cut(s) 168, 202, 617, 661, 705, 765, 788
BstF5I GGATG 3 cut(s) 28, 306, 650
BstFNI CGCG 1 cut(s) 758
BstKTI GATC 4 cut(s) 208, 417, 711, 780
BstMBI GATC 4 cut(s) 205, 414, 708, 777
BstMWI GCNNNNNNNGC 4 cut(s) 70, 189, 286, 613
BstNI CCWGG 2 cut(s) 55, 60
BstNSI RCATGY 2 cut(s) 359, 733
BstSCI CCNGG 3 cut(s) 53, 58, 800
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 758
BstV1I GCAGC 1 cut(s) 486
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 205, 414
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 2 cut(s) 205, 414
BsuRI GGCC 1 cut(s) 58
BtsCI GGATG 3 cut(s) 28, 306, 650
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 6, 323
CciI TCATGA 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 5 cut(s) 196, 370, 397, 571, 628
CviAII CATG 6 cut(s) 19, 356, 382, 553, 655, 730
CviJI RGCY 9 cut(s) 8, 58, 64, 310, 325, 446, 484, 621, 787
CviKI_1 RGCY 9 cut(s) 8, 58, 64, 310, 325, 446, 484, 621, 787
CviQI GTAC 5 cut(s) 196, 370, 397, 571, 628
DdeI CTNAG 7 cut(s) 168, 202, 617, 661, 705, 765, 788
DpnI GATC 4 cut(s) 207, 416, 710, 779
DpnII GATC 4 cut(s) 205, 414, 708, 777
EciI GGCGGA 2 cut(s) 60, 141
Eco57I CTGAAG 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 53, 58
EcoT22I ATGCAT 1 cut(s) 735
FaeI CATG 6 cut(s) 22, 359, 385, 556, 658, 733
FatI CATG 6 cut(s) 18, 355, 381, 552, 654, 729
FauNDI CATATG 1 cut(s) 644
FbaI TGATCA 1 cut(s) 777
Fnu4HI GCNGC 1 cut(s) 500
FokI GGATG 3 cut(s) 35, 313, 637
Fsp4HI GCNGC 1 cut(s) 500
FspBI CTAG 4 cut(s) 422, 548, 602, 749
GluI GCNGC 1 cut(s) 500
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 1 cut(s) 58
HapII CCGG 2 cut(s) 5, 801
Hin1II CATG 6 cut(s) 22, 359, 385, 556, 658, 733
HinfI GANTC 3 cut(s) 28, 114, 609
HpaII CCGG 2 cut(s) 5, 801
Hpy166II GTNNAC 2 cut(s) 229, 573
Hpy188I TCNGA 2 cut(s) 205, 245
Hpy188III TCNNGA 3 cut(s) 111, 382, 816
Hpy8I GTNNAC 2 cut(s) 229, 573
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 1 cut(s) 716
HpyCH4IV ACGT 2 cut(s) 240, 395
HpyCH4V TGCA 4 cut(s) 280, 392, 725, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 189, 286, 613
HpyF3I CTNAG 7 cut(s) 168, 202, 617, 661, 705, 765, 788
HpySE526I ACGT 2 cut(s) 240, 395
Hsp92II CATG 6 cut(s) 22, 359, 385, 556, 658, 733
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 777
Kzo9I GATC 4 cut(s) 205, 414, 708, 777
LmnI GCTCC 1 cut(s) 101
Lsp1109I GCAGC 1 cut(s) 486
LweI GCATC 5 cut(s) 486, 518, 675, 712, 742
MaeI CTAG 4 cut(s) 422, 548, 602, 749
MaeII ACGT 2 cut(s) 240, 395
MalI GATC 4 cut(s) 207, 416, 710, 779
MboI GATC 4 cut(s) 205, 414, 708, 777
MboII GAAGA 3 cut(s) 268, 510, 661
MflI RGATCY 2 cut(s) 205, 414
MhlI GDGCHC 2 cut(s) 291, 662
MluCI AATT 8 cut(s) 75, 80, 150, 261, 342, 451, 684, 805
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 7 cut(s) 116, 219, 291, 311, 348, 436, 529
Mph1103I ATGCAT 1 cut(s) 735
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 2 cut(s) 66, 687
MslI CAYNNNNRTG 2 cut(s) 17, 655
MspI CCGG 2 cut(s) 5, 801
MspR9I CCNGG 3 cut(s) 55, 60, 802
MvaI CCWGG 2 cut(s) 55, 60
MvnI CGCG 1 cut(s) 758
MwoI GCNNNNNNNGC 4 cut(s) 70, 189, 286, 613
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 802
NdeI CATATG 1 cut(s) 644
NdeII GATC 4 cut(s) 205, 414, 708, 777
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 22, 359, 385, 556, 658, 733
NlaIV GGNNCC 3 cut(s) 38, 437, 540
NsiI ATGCAT 1 cut(s) 735
NspI RCATGY 2 cut(s) 359, 733
PagI TCATGA 1 cut(s) 381
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 28, 609
PkrI GCNGC 1 cut(s) 501
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 53, 58
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 2 cut(s) 53, 58
PspN4I GGNNCC 3 cut(s) 38, 437, 540
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PsuI RGATCY 2 cut(s) 205, 414
RsaI GTAC 5 cut(s) 197, 371, 398, 572, 629
RsaNI GTAC 5 cut(s) 196, 370, 397, 571, 628
RseI CAYNNNNRTG 2 cut(s) 17, 655
SaqAI TTAA 2 cut(s) 66, 687
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 4 cut(s) 205, 414, 708, 777
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 3 cut(s) 55, 60, 802
SduI GDGCHC 2 cut(s) 291, 662
SetI ASST 6 cut(s) 243, 398, 427, 544, 623, 754
SfaNI GCATC 5 cut(s) 486, 518, 675, 712, 742
SmiMI CAYNNNNRTG 2 cut(s) 17, 655
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 8 cut(s) 75, 80, 150, 261, 342, 451, 684, 805
SsiI CCGC 3 cut(s) 71, 126, 596
SspMI CTAG 4 cut(s) 422, 548, 602, 749
StyD4I CCNGG 3 cut(s) 53, 58, 800
TaaI ACNGT 1 cut(s) 716
TaiI ACGT 2 cut(s) 243, 398
TaqI TCGA 1 cut(s) 121
TasI AATT 8 cut(s) 75, 80, 150, 261, 342, 451, 684, 805
TatI WGTACW 2 cut(s) 195, 369
TfiI GAWTC 2 cut(s) 28, 609
Tru1I TTAA 2 cut(s) 66, 687
Tru9I TTAA 2 cut(s) 66, 687
TscAI CASTG 1 cut(s) 296
TseI GCWGC 1 cut(s) 499
TspDTI ATGAA 1 cut(s) 370
TspRI CASTG 1 cut(s) 296
XapI RAATTY 1 cut(s) 150
XceI RCATGY 2 cut(s) 359, 733
XspI CTAG 4 cut(s) 422, 548, 602, 749
ZrmI AGTACT 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.