pycom10g13730

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017722
Physical Location & Seq
Reverse (-)
17200191 .. 17201714
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
ATGGAGTTTATTAAGGTTGATGATGGAGATTATTACAATGAGCCGGTGAGTCCTACTGGGCAATGTCTCAACACGTCGGTATTATCCATGTCAGTTCTTGGTGTTTTAGAATCTGAGGTTCCAATCGATGAATCAAAGATCATGTCATTCCTTCAGGATGTCTTCCTCCACATCAACCCACGCTTCTGCTCCATCATGGTTGACGTCGACGGAGAGAAACAATGGAAGAAGGTTGAGGTCAAGCTCACGGATCATGTTCACGTCCCTATTTTTCCCTCTGGAGTATTGTCACCTGAGTCTTACGACCTTTATCTCGGTGACTACATATCAAAAGTAGTACTGGAAAAATACCCAGAAGACAAACCACTGTGGGAAGTTCACATAGTCAAATATCCAACTTCCACTGCCGCTGGTAACCTAATATTCAAGCTTCACCATGCACTTGGCGATGGCTACTCCTTCATGAGTGCTCTTCTTTCTTGTCTGCAAAGAGCTGACGATCCTTCTCGCCCTCTAACTTTTCCGTCGCGCCGAGGTTCCGAGAGAAAGGGACGTGACGTTTATTATAAGAGTGCTTTGAGGTGGGTGTCTCAGAATTTGGCCTCAGCTTTTTATGCCGTGAAGGATTTGGGTTGGAACGCTTTCGTTGAAGATGATCGAACACCGATAAGATCTGGGAATTCTGGAGTTGAGTTTCTGCCAATGGATATATCAAGCTTGATATTCTCCCTTGATGAAATCAAACTAATCAAGAACAAGCTCAACGTGACGATAAATGATGTGATATGTGGAATAGTGTTCTTTGCTACTAGACTGTACATGCAAGAGATGATTAATAATAACCAAAAATCAAGCAGCGCAGCAAGCTGCCGAGCGCTGGTGTTGGTCAATACTAGGATCACTCATGGTGATTATAAGCCGGTGAAGCAGATCGATCGGACCCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

317

Amino Acids

35.74

Weight (kDa)

5.84

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 106 - 262 1.3e-07 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 567, 915
AatII GACGTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 529
AciI CCGC 1 cut(s) 408
AclWI GGATC 3 cut(s) 258, 494, 905
AcsI RAATTY 2 cut(s) 595, 679
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 339, 818
AfeI AGCGCT 1 cut(s) 876
AfiI CCNNNNNNNGG 1 cut(s) 877
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 2 cut(s) 427, 650
AjiI CACGTC 3 cut(s) 75, 262, 554
AluBI AGCT 7 cut(s) 244, 430, 494, 608, 717, 760, 867
AluI AGCT 7 cut(s) 244, 430, 494, 608, 717, 760, 867
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 2 cut(s) 71, 594
AlwI GGATC 3 cut(s) 258, 494, 905
Aor51HI AGCGCT 1 cut(s) 876
AoxI GGCC 1 cut(s) 600
ApeKI GCWGC 3 cut(s) 855, 860, 867
ApoI RAATTY 2 cut(s) 595, 679
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 3 cut(s) 531, 860, 877
AspS9I GGNCC 1 cut(s) 939
AsuHPI GGTGA 6 cut(s) 58, 282, 329, 425, 920, 934
AvaII GGWCC 1 cut(s) 939
BbsI GAAGAC 2 cut(s) 154, 363
Bbv12I GWGCWC 1 cut(s) 472
BbvCI CCTCAGC 1 cut(s) 604
BbvI GCAGC 3 cut(s) 854, 867, 872
BccI CCATC 3 cut(s) 17, 200, 443
BceAI ACGGC 1 cut(s) 602
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BcoDI GTCTC 2 cut(s) 71, 594
BfaI CTAG 2 cut(s) 810, 894
BfoI RGCGCY 1 cut(s) 878
BglII AGATCT 1 cut(s) 671
BisI GCNGC 4 cut(s) 408, 856, 861, 868
BlsI GCNGC 4 cut(s) 409, 857, 862, 869
BmcAI AGTACT 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 939
BmgBI CACGTC 3 cut(s) 75, 262, 554
BmgT120I GGNCC 1 cut(s) 939
BmiI GGNNCC 3 cut(s) 120, 538, 941
BmrI ACTGGG 1 cut(s) 66
BmuI ACTGGG 1 cut(s) 66
BpiI GAAGAC 2 cut(s) 154, 363
BpmI CTGGAG 2 cut(s) 300, 705
Bpu10I CCTNAGC 1 cut(s) 604
Bsa29I ATCGAT 2 cut(s) 126, 933
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 1 cut(s) 877
Bse118I RCCGGY 2 cut(s) 43, 919
Bse1I ACTGG 2 cut(s) 61, 345
Bse3DI GCAATG 1 cut(s) 68
BseCI ATCGAT 2 cut(s) 126, 933
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 877
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 4 cut(s) 105, 285, 605, 618
BseNI ACTGG 2 cut(s) 61, 345
BseXI GCAGC 3 cut(s) 854, 867, 872
Bsh1236I CGCG 1 cut(s) 529
Bsh1285I CGRYCG 1 cut(s) 937
BshFI GGCC 1 cut(s) 602
BshVI ATCGAT 2 cut(s) 126, 933
BsiEI CGRYCG 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 2 cut(s) 44, 920
BslFI GGGAC 2 cut(s) 248, 564
BslI CCNNNNNNNGG 1 cut(s) 877
BsmAI GTCTC 2 cut(s) 71, 594
BsmFI GGGAC 2 cut(s) 248, 564
BsnI GGCC 1 cut(s) 602
Bsp1286I GDGCHC 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
BspACI CCGC 1 cut(s) 408
BspANI GGCC 1 cut(s) 602
BspCNI CTCAG 4 cut(s) 106, 286, 604, 617
BspDI ATCGAT 2 cut(s) 126, 933
BspFNI CGCG 1 cut(s) 529
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 120, 538, 941
BspPI GGATC 3 cut(s) 258, 494, 905
BspQI GCTCTTC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 68
BsrFI RCCGGY 2 cut(s) 43, 919
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 61, 345
BssAI RCCGGY 2 cut(s) 43, 919
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
BssNI GRCGYC 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 369, 816
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 865
BstDEI CTNAG 4 cut(s) 114, 294, 591, 604
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 529
BstH2I RGCGCY 1 cut(s) 878
BstHHI GCGC 3 cut(s) 531, 860, 877
BstKTI GATC 8 cut(s) 141, 253, 502, 658, 674, 900, 933, 937
BstMAI GTCTC 2 cut(s) 71, 594
BstMBI GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
BstMCI CGRYCG 1 cut(s) 937
BstMWI GCNNNNNNNGC 3 cut(s) 614, 864, 925
BstNSI RCATGY 1 cut(s) 823
BstPI GGTNACC 1 cut(s) 413
BstUI CGCG 1 cut(s) 529
BstV1I GCAGC 3 cut(s) 854, 867, 872
BstV2I GAAGAC 2 cut(s) 154, 363
BstX2I RGATCY 1 cut(s) 671
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 1 cut(s) 671
Bsu15I ATCGAT 2 cut(s) 126, 933
BsuRI GGCC 1 cut(s) 602
BsuTUI ATCGAT 2 cut(s) 126, 933
BtgZI GCGATG 1 cut(s) 462
BtrI CACGTC 3 cut(s) 75, 262, 554
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 2 cut(s) 365, 402
Cac8I GCNNGC 1 cut(s) 865
CciI TCATGA 1 cut(s) 462
CfoI GCGC 3 cut(s) 531, 860, 877
Cfr10I RCCGGY 2 cut(s) 43, 919
Cfr13I GGNCC 1 cut(s) 939
ClaI ATCGAT 2 cut(s) 126, 933
Csp6I GTAC 2 cut(s) 338, 817
CviAII CATG 8 cut(s) 88, 142, 196, 254, 437, 463, 820, 905
CviQI GTAC 2 cut(s) 338, 817
DdeI CTNAG 4 cut(s) 114, 294, 591, 604
DpnI GATC 8 cut(s) 140, 252, 501, 657, 673, 899, 932, 936
DpnII GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 939
Eco47III AGCGCT 1 cut(s) 876
Eco57I CTGAAG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 679
FaeI CATG 8 cut(s) 91, 145, 199, 257, 440, 466, 823, 908
FaqI GGGAC 2 cut(s) 248, 564
FatI CATG 8 cut(s) 87, 141, 195, 253, 436, 462, 819, 904
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 408, 856, 861, 868
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 408, 856, 861, 868
FspBI CTAG 2 cut(s) 810, 894
GlaI GCGC 3 cut(s) 530, 859, 876
GluI GCNGC 4 cut(s) 408, 856, 861, 868
GsuI CTGGAG 2 cut(s) 300, 705
HaeII RGCGCY 1 cut(s) 878
HaeIII GGCC 1 cut(s) 602
HapII CCGG 2 cut(s) 44, 920
HhaI GCGC 3 cut(s) 531, 860, 877
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 8 cut(s) 91, 145, 199, 257, 440, 466, 823, 908
Hin6I GCGC 3 cut(s) 529, 858, 875
HinP1I GCGC 3 cut(s) 529, 858, 875
HincII GTYRAC 2 cut(s) 202, 208
HindII GTYRAC 2 cut(s) 202, 208
HindIII AAGCTT 2 cut(s) 428, 715
HinfI GANTC 4 cut(s) 49, 110, 131, 296
HpaII CCGG 2 cut(s) 44, 920
HphI GGTGA 6 cut(s) 58, 282, 329, 425, 920, 934
Hpy166II GTNNAC 4 cut(s) 202, 208, 259, 379
Hpy188I TCNGA 5 cut(s) 115, 541, 594, 939, 950
Hpy188III TCNNGA 5 cut(s) 155, 279, 463, 684, 751
Hpy8I GTNNAC 4 cut(s) 202, 208, 259, 379
Hpy99I CGWCG 4 cut(s) 79, 209, 212, 529
HpyAV CCTTC 5 cut(s) 161, 223, 469, 513, 616
HpyCH4III ACNGT 2 cut(s) 369, 816
HpyCH4IV ACGT 6 cut(s) 74, 204, 261, 553, 558, 765
HpyCH4V TGCA 3 cut(s) 440, 487, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 614, 864, 925
HpyF3I CTNAG 4 cut(s) 114, 294, 591, 604
HpySE526I ACGT 6 cut(s) 74, 204, 261, 553, 558, 765
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 8 cut(s) 91, 145, 199, 257, 440, 466, 823, 908
HspAI GCGC 3 cut(s) 529, 858, 875
Kzo9I GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 3 cut(s) 854, 867, 872
MaeI CTAG 2 cut(s) 810, 894
MaeII ACGT 6 cut(s) 74, 204, 261, 553, 558, 765
MaeIII GTNAC 5 cut(s) 288, 317, 413, 554, 766
MalI GATC 8 cut(s) 140, 252, 501, 657, 673, 899, 932, 936
MboI GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
MboII GAAGA 5 cut(s) 154, 238, 368, 464, 662
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 1 cut(s) 472
MluCI AATT 3 cut(s) 595, 679, 944
MlyI GAGTC 2 cut(s) 58, 305
MmeI TCCRAC 2 cut(s) 419, 614
MnlI CCTC 8 cut(s) 109, 176, 229, 286, 522, 527, 573, 613
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 2 cut(s) 12, 834
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 44, 920
MvnI CGCG 1 cut(s) 529
MwoI GCNNNNNNNGC 3 cut(s) 614, 864, 925
NdeII GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
NlaIII CATG 8 cut(s) 91, 145, 199, 257, 440, 466, 823, 908
NlaIV GGNNCC 3 cut(s) 120, 538, 941
NmeAIII GCCGAG 2 cut(s) 557, 896
NmuCI GTSAC 4 cut(s) 288, 317, 554, 766
NspI RCATGY 1 cut(s) 823
PagI TCATGA 1 cut(s) 462
PciSI GCTCTTC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 2 cut(s) 110, 131
PkrI GCNGC 4 cut(s) 409, 857, 862, 869
Ple19I CGATCG 1 cut(s) 937
PleI GAGTC 2 cut(s) 57, 304
PpsI GAGTC 2 cut(s) 57, 304
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 2 cut(s) 567, 915
PspEI GGTNACC 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 120, 538, 941
PspPI GGNCC 1 cut(s) 939
PsuI RGATCY 1 cut(s) 671
PvuI CGATCG 1 cut(s) 937
RsaI GTAC 2 cut(s) 339, 818
RsaNI GTAC 2 cut(s) 338, 817
SalI GTCGAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 12, 834
SatI GCNGC 4 cut(s) 408, 856, 861, 868
Sau3AI GATC 8 cut(s) 138, 250, 499, 655, 671, 897, 930, 934
Sau96I GGNCC 1 cut(s) 939
ScaI AGTACT 1 cut(s) 339
SchI GAGTC 2 cut(s) 58, 305
SduI GDGCHC 1 cut(s) 472
SgrDI CGTCGACG 1 cut(s) 206
SinI GGWCC 1 cut(s) 939
Sse9I AATT 3 cut(s) 595, 679, 944
SsiI CCGC 1 cut(s) 408
SspI AATATT 1 cut(s) 423
SspMI CTAG 2 cut(s) 810, 894
TaaI ACNGT 2 cut(s) 369, 816
TaiI ACGT 6 cut(s) 77, 207, 264, 556, 561, 768
TaqI TCGA 4 cut(s) 126, 207, 658, 933
TasI AATT 3 cut(s) 595, 679, 944
TatI WGTACW 2 cut(s) 337, 816
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 110, 131
Tru1I TTAA 2 cut(s) 12, 834
Tru9I TTAA 2 cut(s) 12, 834
TscAI CASTG 2 cut(s) 372, 409
TseFI GTSAC 4 cut(s) 288, 317, 554, 766
TseI GCWGC 3 cut(s) 855, 860, 867
Tsp45I GTSAC 4 cut(s) 288, 317, 554, 766
TspDTI ATGAA 3 cut(s) 144, 451, 750
TspGWI ACGGA 3 cut(s) 225, 263, 513
TspRI CASTG 2 cut(s) 372, 409
VpaK11BI GGWCC 1 cut(s) 939
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 2 cut(s) 595, 679
XceI RCATGY 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 2 cut(s) 810, 894
ZraI GACGTC 1 cut(s) 205
ZrmI AGTACT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.