pycom10g14140

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010915
Physical Location & Seq
Reverse (-)
17540394 .. 17545536
5143 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2886 bp
ATGAGAAACATAGGTGACCCCACTAAAGAAAGACAATCATTTTCTTGGATCCCAATATCTCTTTCACTTCCTTCTTTTGAAATCTTCACTCTGTTTACTCCCCTTTGTTTCCTAGATAAGTATTTTGGGCTTCTCTGGTTTCTGAAGATAGCAAACTTTCGTGGTTCCGAATTCATGGACCTCCATTGTTTGTGGGTTGGCATCTGTCTGTTCTTCGGCTTTTCGCTAGTTGCCTTATGTGACCAAGATGGTTTCTTGAGCTTGATTTGCGGTGGAACTGCCGATTACACCGATTCATCTTCAATCTCATGGATTCCAGACAGTGCGTACATCGCCACAGGCAACATAACCAATGTTGATTACATCGAAGGAACTTCCTCATCTAGTGTCCCAGTTCGGTACTTCCCAATTTCCCAAGGCCGGAATTGTTATAAGCTACCAGTCACCAATGTGTCGTCTTTGGTTCTTGTGAGAGCTCAGTTTGTATATAAAAACTATGACGGGCATCGAAAGCCCCCGTCATTCTCCGTCTCTCTTGGGACAGCCATTGTTAGTACTGTAGACCTCCTAAAAAATGATCCGTGGACAGAAGAGTTTGTTTGGCCAGTTGATAAGGACACAGTGTCGTTCTGTTTGCACGAAATTGCAGGTAGAGGAGCTCCTGTAATTTCTACACTTGAAGTTAGGCCACTTCCTCAAGAAGCTTACACAAGTGGCATGGAAGATTTCCCTAACAAGTCACTTAGGAAAAACTACCGGATAAATTGCGGTTACACGAATGGAACTTTGAGGTACCCTTTGGATCCGTATGATCGGATATGGGATGCTGATCAAAGCTTTTCACCATTTCATACATCCACCGAGTTCAAGACTCAGCTTAGCTTCAATTTCTCTGCTTTGAAGGAGGCTCCCCCTGCTGCCATTCTCCAAACAGCAAGAGTTTTGGCGCGAAGGGACATGTTGACATATACCTTTCCTCTTGATACCCTGGCAGACTACTACATTGTCCTCTACTTTGCTGGGATTCTTCCTGTTTTTCCTTCGTTTGATATCTTAATCAACGGTGCTGTTGTGCAGTCAAACTATACCATCAGAAGCTCAGAAGTTAGAACACTATATTTTAAACAGAGAGGAATAAAGAGCTTGAACGTCACACTGAAAACCATCAGCTTCTATCCTCAAGTCAACGCAATTGAGGTGTATGAAATTCTCGATGTTCCAGAGGAAGCCTCCTCCACTACAGTATCAGCTCTTCAGGTTATCCAACAGTCTACCGGTTTAGATTTGGGGTGGCAAGATGATCCTTGCTCTCCTAAATCATGGGATCAAATTGGATGTGAAGGAAACATAGTCACATCACTGGAGCTTCCGGGAATCAGTTTGAGGTCGATTAGTGCAGCCATCGGCGATTTGCTTGATCTTAAAACATTGGATCTGCATAACACATCACTTGCCGGTGAAATACAGAATTTGGGCAGCTTGACACGCCTCGAGAAATTGAATCTGAGCTTCAATCGACTAACATCCTTCGGTACTGAGTTGGAGAACTTGGTTAGCCTCCAAATTCTGGACTTGCAAAATAATACTTTGCAGGGAATCGTTCCAGACAGCTTGGGAGAGTTAGAAGAACTTCACTTATTGAACCTGGAAAACAACAAACTACAAGGCACTCTTCCGCTGACCTTGAACCGAGAAAGTTTGGAAATCAGGACATCTGGAAATTTATGCCTCTCTTTTTCGACAATGTCATGCAATGATCCTTCATCCAACTCTTCAATTGAGGCACCACAAGTTACACTCTTTCCCCGAAAGAAACACACTGAACACAGCCATGTAGCAATTATACTTGGCGCAATTGGAGGAGCCTTACTTGCACTTGCGATATTTGTCTTCCTTTTAGTATTCTTGCACATGAAGAAAAGGAGAACTGAAATGACTTGCGCGGCAAGGGAGGTGGCGGATATGCGAAACTGGAATGCTGCAAGAGTTTTTACCTACAAAGAAATCAAGGCAGCTACAAACAATTTTAAGGAGGTTCTAGGCCGGGGTAGTTTCGGTTGTGTTTACCTTGGAAAGCTTTCGGATCGAAAAATGGTGGCTGTGAAAGTGCGATTCGATAAAAGCCAACTGGGGGCAGATTCTTTTATCAACGAGGTGCGTCTCTTGTCGGGAATTAGCCATCAAAATCTTGTAGGCTTGGAAGGATTCTGTCATGAAGCAAAGCAGCAAATACTTGTTTATGAGTATCTTCCTGGCGGATCGTTGGCTAACCATCTTTACGGTCCAAAGAGTAAAAAAGTTTCGCTGAGTTGGGTTCGCCGGCTGAAAATTGCTGTCGATGCTGCAAAAGGATTGGACTATCTACACAACGGGAATGAGCCGCGAGTCATACACCGTGATGTGAAATGCAGTAACATCCTTCTGGACAAGGAGATGAATGCTAAGGTTTGTGATTTTGGCCTCTCTAAGCAAGTAATGCAAGCGGAAGCAACTCATGTGACCACTGTCGTTAAGGGCACTGCCGGCTATATTGATCCTGAATATTACTCGACCCAGCAGCTTACCGAGAAAAGTGACGTTTATAGCTTTGGAGTTGTTCTTCTGGAGCTGATTTGCGGGAGAGAACCATTGAGTCACTCTGGAACCCCCGACTCCTTTAACCTGGTGTTATGGGCCAAGCCTTACTTACAGGCAGGTGCATATGAGATAGTGGATGAGAGCCTGCAGGGCACATTCGATGCCGAAAGCATGAGAAAGGCAGCCTTAGTTGCTATAAAATCTGTAGAGAGAGATGCATCACAAAGGCCAACCATAGCAGAGGTTTTGGCCGAGCTCAAAGAAGCCTTCAGCATTCAACTTTCGTATCTCGCATCACGTGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

962

Amino Acids

106.99

Weight (kDa)

5.76

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 90 - 402 2.4e-55 Malectin-like domain
LRR_8 PF13855 496 - 554 7.3e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 676 - 943 2.1e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 676 - 943 2.4e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010915)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48740
fragaria_vesca FvH4_2g19020 FvH4_2g19020 FvH4_2g19020 FvH4_2g19020
malus_domestica MD10G1164000.v1.1
prunus_persica Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1
pyrus_communis pycom10g14140
rosa_chinensis RchiOBHm_Chr6g0284821
rosa_laevigata RLG00000012713
rosa_multiflora Rmu_sc0000686.1_g000012
rosa_roxburghii Rroxscaffold_7G00184340
rosa_rugosa Rorug06G0167200
rosa_samantha Rh6AG279900 Rh6BG279900 Rh6CG282100 Rh6DG275000
rosa_wichuraiana Rw6G024060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AarI CACCTGC 1 cut(s) 2684
Acc36I ACCTGC 2 cut(s) 638, 2684
Acc65I GGTACC 1 cut(s) 792
AccB1I GGYRCC 2 cut(s) 792, 1783
AccB7I CCANNNNNTGG 1 cut(s) 1567
AccI GTMKAC 2 cut(s) 561, 1271
AccII CGCG 3 cut(s) 949, 1943, 2383
AciI CCGC 9 cut(s) 270, 768, 1676, 1943, 1958, 2256, 2381, 2483, 2616
AcoI YGGCCR 2 cut(s) 602, 2826
AcsI RAATTY 5 cut(s) 170, 1206, 1468, 1563, 1720
AcuI CTGAAG 3 cut(s) 164, 1238, 2830
AcvI CACGTG 1 cut(s) 2876
AfaI GTAC 5 cut(s) 329, 401, 556, 794, 1534
AfiI CCNNNNNNNGG 3 cut(s) 420, 1567, 2131
AflIII ACRYGT 2 cut(s) 957, 2880
AgeI ACCGGT 1 cut(s) 1274
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 4 cut(s) 987, 1644, 2251, 2661
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AleI CACNNNNGTG 1 cut(s) 449
Alw21I GWGCWC 3 cut(s) 478, 661, 2835
Alw26I GTCTC 2 cut(s) 535, 2165
Ama87I CYCGRG 1 cut(s) 1490
AoxI GGCC 8 cut(s) 418, 602, 686, 2041, 2458, 2673, 2804, 2826
ApeKI GCWGC 9 cut(s) 917, 1397, 1476, 1979, 2012, 2224, 2342, 2557, 2759
ApoI RAATTY 5 cut(s) 170, 1206, 1468, 1563, 1720
AsiGI ACCGGT 1 cut(s) 1274
Asp700I GAANNNNTTC 2 cut(s) 1629, 2077
Asp718I GGTACC 1 cut(s) 792
AspLEI GCGC 3 cut(s) 949, 1853, 1943
AspS9I GGNCC 3 cut(s) 178, 2282, 2673
AsuC2I CCSGG 2 cut(s) 1371, 2045
AsuHPI GGTGA 4 cut(s) 26, 436, 834, 1469
AvaI CYCGRG 1 cut(s) 1490
AvaII GGWCC 2 cut(s) 178, 2282
BaeGI GKGCMC 2 cut(s) 2519, 2732
BaeI ACNNNNGTAYC 4 cut(s) 1227, 1260, 2846, 2879
BalI TGGCCA 1 cut(s) 604
BamHI GGATCC 2 cut(s) 48, 802
BanI GGYRCC 2 cut(s) 792, 1783
BanII GRGCYC 3 cut(s) 478, 661, 2835
BarI GAAGNNNNNNTAC 2 cut(s) 1236, 1268
BbrPI CACGTG 1 cut(s) 2876
BbsI GAAGAC 1 cut(s) 1882
Bbv12I GWGCWC 3 cut(s) 478, 661, 2835
BbvI GCAGC 9 cut(s) 904, 1409, 1488, 1966, 2024, 2236, 2329, 2569, 2771
BccI CCATC 6 cut(s) 242, 1097, 1172, 1409, 2187, 2280
BciT130I CCWGG 4 cut(s) 989, 1646, 2253, 2663
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 2 cut(s) 1371, 2045
BcoDI GTCTC 2 cut(s) 535, 2165
BfaI CTAG 4 cut(s) 113, 227, 384, 2039
BfmI CTRYAG 4 cut(s) 558, 1239, 2723, 2781
BfuAI ACCTGC 2 cut(s) 638, 2684
BglI GCCNNNNNGGC 1 cut(s) 2727
BlpI GCTNAGC 1 cut(s) 878
BmcAI AGTACT 1 cut(s) 556
Bme1390I CCNGG 6 cut(s) 989, 1371, 1646, 2045, 2253, 2663
Bme18I GGWCC 2 cut(s) 178, 2282
BmeRI GACNNNNNGTC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 1490
BmgT120I GGNCC 3 cut(s) 178, 2282, 2673
BmiI GGNNCC 8 cut(s) 50, 166, 794, 804, 909, 1785, 1864, 2644
BmrFI CCNGG 6 cut(s) 989, 1371, 1646, 2045, 2253, 2663
BmrI ACTGGG 2 cut(s) 386, 2138
BmsI GCATC 8 cut(s) 210, 514, 814, 2329, 2728, 2782, 2804, 2879
BmuI ACTGGG 2 cut(s) 386, 2138
BoxI GACNNNNGTC 1 cut(s) 2504
BpiI GAAGAC 1 cut(s) 1882
BplI GAGNNNNNCTC 2 cut(s) 1214, 1246
BpmI CTGGAG 2 cut(s) 1382, 2624
Bpu10I CCTNAGC 1 cut(s) 2442
Bpu1102I GCTNAGC 1 cut(s) 878
BpuEI CTTGAG 3 cut(s) 277, 681, 1164
BpuMI CCSGG 2 cut(s) 1371, 2045
BsaAI YACGTR 1 cut(s) 2876
BsaBI GATNNNNATC 1 cut(s) 828
BsaJI CCNNGG 5 cut(s) 415, 581, 987, 2044, 2068
BsaWI WCCGGW 2 cut(s) 756, 1274
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 3 cut(s) 420, 1567, 2131
Bse118I RCCGGY 4 cut(s) 1274, 1454, 2319, 2522
Bse1I ACTGG 6 cut(s) 392, 440, 605, 1365, 1976, 2133
Bse3DI GCAATG 1 cut(s) 1759
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 4 cut(s) 989, 1646, 2253, 2663
BseDI CCNNGG 5 cut(s) 415, 581, 987, 2044, 2068
BseGI GGATG 7 cut(s) 829, 854, 1340, 1523, 1763, 2415, 2719
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 3 cut(s) 420, 1567, 2131
BseMI GCAATG 1 cut(s) 1759
BseMII CTCAG 6 cut(s) 491, 887, 1113, 1496, 1527, 2297
BseNI ACTGG 6 cut(s) 392, 440, 605, 1365, 1976, 2133
BseRI GAGGAG 3 cut(s) 669, 1222, 1875
BseSI GKGCMC 2 cut(s) 2519, 2732
BseXI GCAGC 9 cut(s) 904, 1409, 1488, 1966, 2024, 2236, 2329, 2569, 2771
BseYI CCCAGC 2 cut(s) 1019, 2553
BsgI GTGCAG 2 cut(s) 1094, 1416
Bsh1236I CGCG 3 cut(s) 949, 1943, 2383
BshFI GGCC 8 cut(s) 420, 604, 688, 2043, 2460, 2675, 2806, 2828
BshNI GGYRCC 2 cut(s) 792, 1783
BshTI ACCGGT 1 cut(s) 1274
BsiHKAI GWGCWC 3 cut(s) 478, 661, 2835
BsiHKCI CYCGRG 1 cut(s) 1490
BsiSI CCGG 8 cut(s) 421, 757, 1275, 1370, 1455, 2044, 2320, 2523
BslFI GGGAC 3 cut(s) 374, 553, 968
BslI CCNNNNNNNGG 3 cut(s) 420, 1567, 2131
BsmAI GTCTC 2 cut(s) 535, 2165
BsmBI CGTCTC 2 cut(s) 535, 2165
BsmFI GGGAC 3 cut(s) 374, 553, 968
BsmI GAATGC 3 cut(s) 1981, 2443, 2850
BsnI GGCC 8 cut(s) 420, 604, 688, 2043, 2460, 2675, 2806, 2828
BsoBI CYCGRG 1 cut(s) 1490
Bsp1286I GDGCHC 5 cut(s) 478, 661, 2519, 2732, 2835
Bsp1720I GCTNAGC 1 cut(s) 878
BspACI CCGC 9 cut(s) 270, 768, 1676, 1943, 1958, 2256, 2381, 2483, 2616
BspANI GGCC 8 cut(s) 420, 604, 688, 2043, 2460, 2675, 2806, 2828
BspCNI CTCAG 6 cut(s) 490, 886, 1112, 1497, 1528, 2298
BspFNI CGCG 3 cut(s) 949, 1943, 2383
BspHI TCATGA 1 cut(s) 2212
BspLI GGNNCC 8 cut(s) 50, 166, 794, 804, 909, 1785, 1864, 2644
BspMAI CTGCAG 1 cut(s) 2727
BspMI ACCTGC 2 cut(s) 638, 2684
BspQI GCTCTTC 1 cut(s) 1257
BspT107I GGYRCC 2 cut(s) 792, 1783
BsrDI GCAATG 1 cut(s) 1759
BsrFI RCCGGY 4 cut(s) 1274, 1454, 2319, 2522
BsrI ACTGG 6 cut(s) 392, 440, 605, 1365, 1976, 2133
BssAI RCCGGY 4 cut(s) 1274, 1454, 2319, 2522
BssECI CCNNGG 5 cut(s) 415, 581, 987, 2044, 2068
BssT1I CCWWGG 2 cut(s) 415, 2068
Bst2UI CCWGG 4 cut(s) 989, 1646, 2253, 2663
Bst4CI ACNGT 9 cut(s) 323, 559, 622, 1064, 1243, 1269, 2282, 2396, 2506
Bst6I CTCTTC 4 cut(s) 585, 1257, 1677, 1777
BstAPI GCANNNNNTGC 1 cut(s) 2476
BstBAI YACGTR 1 cut(s) 2876
BstC8I GCNNGC 4 cut(s) 2321, 2481, 2524, 2723
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 7 cut(s) 829, 854, 1340, 1523, 1763, 2415, 2719
BstFNI CGCG 3 cut(s) 949, 1943, 2383
BstHHI GCGC 3 cut(s) 949, 1853, 1943
BstMAI GTCTC 2 cut(s) 535, 2165
BstNI CCWGG 4 cut(s) 989, 1646, 2253, 2663
BstNSI RCATGY 2 cut(s) 961, 2884
BstPAI GACNNNNGTC 1 cut(s) 2504
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 6 cut(s) 987, 1369, 1644, 2043, 2251, 2661
BstSFI CTRYAG 4 cut(s) 558, 1239, 2723, 2781
BstSLI GKGCMC 2 cut(s) 2519, 2732
BstUI CGCG 3 cut(s) 949, 1943, 2383
BstV1I GCAGC 9 cut(s) 904, 1409, 1488, 1966, 2024, 2236, 2329, 2569, 2771
BstV2I GAAGAC 1 cut(s) 1882
BstX2I RGATCY 3 cut(s) 48, 802, 1432
BstYI RGATCY 3 cut(s) 48, 802, 1432
BsuRI GGCC 8 cut(s) 420, 604, 688, 2043, 2460, 2675, 2806, 2828
BtgI CCRYGG 1 cut(s) 581
BtgZI GCGATG 1 cut(s) 316
BtsCI GGATG 7 cut(s) 829, 854, 1340, 1523, 1763, 2415, 2719
BtsI GCAGTG 1 cut(s) 2517
BtsIMutI CAGTG 7 cut(s) 328, 627, 1154, 1358, 1818, 2502, 2517
BveI ACCTGC 2 cut(s) 638, 2684
Cac8I GCNNGC 4 cut(s) 2321, 2481, 2524, 2723
CciI TCATGA 1 cut(s) 2212
CfoI GCGC 3 cut(s) 949, 1853, 1943
Cfr10I RCCGGY 4 cut(s) 1274, 1454, 2319, 2522
Cfr13I GGNCC 3 cut(s) 178, 2282, 2673
CseI GACGC 1 cut(s) 2147
CsiI ACCWGGT 1 cut(s) 2661
Csp6I GTAC 5 cut(s) 328, 400, 555, 793, 1533
CspAI ACCGGT 1 cut(s) 1274
CspCI CAANNNNNGTGG 2 cut(s) 1935, 1970
CviQI GTAC 5 cut(s) 328, 400, 555, 793, 1533
DraI TTTAAA 1 cut(s) 1123
DriI GACNNNNNGTC 1 cut(s) 622
EaeI YGGCCR 2 cut(s) 602, 2826
Eam1104I CTCTTC 4 cut(s) 585, 1257, 1677, 1777
Eam1105I GACNNNNNGTC 1 cut(s) 622
EarI CTCTTC 4 cut(s) 585, 1257, 1677, 1777
EciI GGCGGA 2 cut(s) 1973, 2271
Ecl136II GAGCTC 3 cut(s) 476, 659, 2833
Eco130I CCWWGG 2 cut(s) 415, 2068
Eco24I GRGCYC 3 cut(s) 478, 661, 2835
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 2 cut(s) 178, 2282
Eco53kI GAGCTC 3 cut(s) 476, 659, 2833
Eco57I CTGAAG 3 cut(s) 164, 1238, 2830
Eco72I CACGTG 1 cut(s) 2876
Eco88I CYCGRG 1 cut(s) 1490
Eco91I GGTNACC 1 cut(s) 14
EcoICRI GAGCTC 3 cut(s) 476, 659, 2833
EcoO65I GGTNACC 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 4 cut(s) 987, 1644, 2251, 2661
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 2 cut(s) 415, 2068
EcoT22I ATGCAT 1 cut(s) 2797
EcoT38I GRGCYC 3 cut(s) 478, 661, 2835
ErhI CCWWGG 2 cut(s) 415, 2068
Esp3I CGTCTC 2 cut(s) 535, 2165
FalI AAGNNNNNCTT 6 cut(s) 1656, 1688, 2669, 2701, 2747, 2779
FaqI GGGAC 3 cut(s) 374, 553, 968
FauI CCCGC 1 cut(s) 2609
FauNDI CATATG 1 cut(s) 2701
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 2 cut(s) 561, 1271
FokI GGATG 7 cut(s) 836, 841, 1347, 1510, 1750, 2402, 2726
FriOI GRGCYC 3 cut(s) 478, 661, 2835
FspBI CTAG 4 cut(s) 113, 227, 384, 2039
GlaI GCGC 3 cut(s) 948, 1852, 1942
GsaI CCCAGC 2 cut(s) 1023, 2557
GsuI CTGGAG 2 cut(s) 1382, 2624
HaeIII GGCC 8 cut(s) 420, 604, 688, 2043, 2460, 2675, 2806, 2828
HapII CCGG 8 cut(s) 421, 757, 1275, 1370, 1455, 2044, 2320, 2523
HgaI GACGC 1 cut(s) 2147
HhaI GCGC 3 cut(s) 949, 1853, 1943
Hin6I GCGC 3 cut(s) 947, 1851, 1941
HinP1I GCGC 3 cut(s) 947, 1851, 1941
HincII GTYRAC 2 cut(s) 963, 1186
HindII GTYRAC 2 cut(s) 963, 1186
HindIII AAGCTT 3 cut(s) 702, 835, 2075
HpaII CCGG 8 cut(s) 421, 757, 1275, 1370, 1455, 2044, 2320, 2523
HphI GGTGA 4 cut(s) 26, 436, 834, 1469
Hpy166II GTNNAC 7 cut(s) 96, 562, 585, 963, 1186, 1272, 2065
Hpy188I TCNGA 7 cut(s) 144, 169, 816, 1094, 1102, 1506, 2083
Hpy8I GTNNAC 7 cut(s) 96, 562, 585, 963, 1186, 1272, 2065
HpyCH4III ACNGT 9 cut(s) 323, 559, 622, 1064, 1243, 1269, 2282, 2396, 2506
HpyCH4IV ACGT 3 cut(s) 1149, 2577, 2875
HpySE526I ACGT 3 cut(s) 1149, 2577, 2875
HspAI GCGC 3 cut(s) 947, 1851, 1941
KpnI GGTACC 1 cut(s) 796
KroI GCCGGC 2 cut(s) 2319, 2522
KroNI GCCGGC 2 cut(s) 2321, 2524
Ksp22I TGATCA 1 cut(s) 829
LguI GCTCTTC 1 cut(s) 1257
LmnI GCTCC 6 cut(s) 656, 664, 913, 1363, 1862, 2605
Lsp1109I GCAGC 9 cut(s) 904, 1409, 1488, 1966, 2024, 2236, 2329, 2569, 2771
LweI GCATC 8 cut(s) 210, 514, 814, 2329, 2728, 2782, 2804, 2879
MabI ACCWGGT 1 cut(s) 2661
MaeI CTAG 4 cut(s) 113, 227, 384, 2039
MaeII ACGT 3 cut(s) 1149, 2577, 2875
MfeI CAATTG 3 cut(s) 1191, 1776, 1854
MflI RGATCY 3 cut(s) 48, 802, 1432
MhlI GDGCHC 5 cut(s) 478, 661, 2519, 2732, 2835
MlsI TGGCCA 1 cut(s) 604
MluNI TGGCCA 1 cut(s) 604
MlyI GAGTC 4 cut(s) 865, 2394, 2641, 2645
MmeI TCCRAC 3 cut(s) 1288, 1521, 1791
Mox20I TGGCCA 1 cut(s) 604
Mph1103I ATGCAT 1 cut(s) 2797
MroNI GCCGGC 2 cut(s) 2319, 2522
MroXI GAANNNNTTC 2 cut(s) 1629, 2077
MscI TGGCCA 1 cut(s) 604
MseI TTAA 6 cut(s) 1055, 1122, 1422, 2028, 2511, 2658
MslI CAYNNNNRTG 3 cut(s) 449, 1830, 2397
Msp20I TGGCCA 1 cut(s) 604
MspA1I CMGCKG 1 cut(s) 1678
MspI CCGG 8 cut(s) 421, 757, 1275, 1370, 1455, 2044, 2320, 2523
MspR9I CCNGG 6 cut(s) 989, 1371, 1646, 2045, 2253, 2663
MunI CAATTG 3 cut(s) 1191, 1776, 1854
Mva1269I GAATGC 3 cut(s) 1981, 2443, 2850
MvaI CCWGG 4 cut(s) 989, 1646, 2253, 2663
MvnI CGCG 3 cut(s) 949, 1943, 2383
NaeI GCCGGC 2 cut(s) 2321, 2524
NciI CCSGG 2 cut(s) 1371, 2045
NdeI CATATG 1 cut(s) 2701
NgoMIV GCCGGC 2 cut(s) 2319, 2522
NlaIV GGNNCC 8 cut(s) 50, 166, 794, 804, 909, 1785, 1864, 2644
NmeAIII GCCGAG 1 cut(s) 2854
NsiI ATGCAT 1 cut(s) 2797
NspI RCATGY 2 cut(s) 961, 2884
OliI CACNNNNGTG 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 1490
PagI TCATGA 1 cut(s) 2212
PaqCI CACCTGC 1 cut(s) 2684
PciI ACATGT 2 cut(s) 957, 2880
PciSI GCTCTTC 1 cut(s) 1257
PctI GAATGC 3 cut(s) 1981, 2443, 2850
PdiI GCCGGC 2 cut(s) 2321, 2524
PdmI GAANNNNTTC 2 cut(s) 1629, 2077
PfeI GAWTC 9 cut(s) 293, 313, 1024, 1374, 1501, 1596, 2112, 2138, 2205
PflFI GACNNNGTC 1 cut(s) 1744
PflMI CCANNNNNTGG 1 cut(s) 1567
PfoI TCCNGGA 1 cut(s) 1369
PinAI ACCGGT 1 cut(s) 1274
PleI GAGTC 4 cut(s) 865, 2393, 2640, 2645
PmaCI CACGTG 1 cut(s) 2876
PmlI CACGTG 1 cut(s) 2876
PpsI GAGTC 4 cut(s) 865, 2393, 2640, 2645
Ppu21I YACGTR 1 cut(s) 2876
PscI ACATGT 2 cut(s) 957, 2880
PshAI GACNNNNGTC 1 cut(s) 2504
PsiI TTATAA 1 cut(s) 432
Psp124BI GAGCTC 3 cut(s) 478, 661, 2835
Psp6I CCWGG 4 cut(s) 987, 1644, 2251, 2661
PspCI CACGTG 1 cut(s) 2876
PspEI GGTNACC 1 cut(s) 14
PspFI CCCAGC 2 cut(s) 1019, 2553
PspGI CCWGG 4 cut(s) 987, 1644, 2251, 2661
PspN4I GGNNCC 8 cut(s) 50, 166, 794, 804, 909, 1785, 1864, 2644
PspPI GGNCC 3 cut(s) 178, 2282, 2673
PstI CTGCAG 1 cut(s) 2727
PsuI RGATCY 3 cut(s) 48, 802, 1432
PsyI GACNNNGTC 1 cut(s) 1744
RsaI GTAC 5 cut(s) 329, 401, 556, 794, 1534
RsaNI GTAC 5 cut(s) 328, 400, 555, 793, 1533
RseI CAYNNNNRTG 3 cut(s) 449, 1830, 2397
SacI GAGCTC 3 cut(s) 478, 661, 2835
SapI GCTCTTC 1 cut(s) 1257
SaqAI TTAA 6 cut(s) 1055, 1122, 1422, 2028, 2511, 2658
Sau96I GGNCC 3 cut(s) 178, 2282, 2673
SbfI CCTGCAGG 1 cut(s) 2727
ScaI AGTACT 1 cut(s) 556
SchI GAGTC 4 cut(s) 865, 2394, 2641, 2645
ScrFI CCNGG 6 cut(s) 989, 1371, 1646, 2045, 2253, 2663
SdaI CCTGCAGG 1 cut(s) 2727
SduI GDGCHC 5 cut(s) 478, 661, 2519, 2732, 2835
SexAI ACCWGGT 1 cut(s) 2661
SfaNI GCATC 8 cut(s) 210, 514, 814, 2329, 2728, 2782, 2804, 2879
SfcI CTRYAG 4 cut(s) 558, 1239, 2723, 2781
Sfr274I CTCGAG 1 cut(s) 1490
SinI GGWCC 2 cut(s) 178, 2282
SlaI CTCGAG 1 cut(s) 1490
SmiMI CAYNNNNRTG 3 cut(s) 449, 1830, 2397
SmlI CTYRAG 4 cut(s) 256, 696, 1179, 1490
SmoI CTYRAG 4 cut(s) 256, 696, 1179, 1490
Sse8387I CCTGCAGG 1 cut(s) 2727
SsiI CCGC 9 cut(s) 270, 768, 1676, 1943, 1958, 2256, 2381, 2483, 2616
SspI AATATT 1 cut(s) 2543
SspMI CTAG 4 cut(s) 113, 227, 384, 2039
SstI GAGCTC 3 cut(s) 478, 661, 2835
StyD4I CCNGG 6 cut(s) 987, 1369, 1644, 2043, 2251, 2661
StyI CCWWGG 2 cut(s) 415, 2068
TaaI ACNGT 9 cut(s) 323, 559, 622, 1064, 1243, 1269, 2282, 2396, 2506
TaiI ACGT 3 cut(s) 1152, 2580, 2878
TatI WGTACW 1 cut(s) 554
TauI GCSGC 2 cut(s) 1946, 2383
TfiI GAWTC 9 cut(s) 293, 313, 1024, 1374, 1501, 1596, 2112, 2138, 2205
Tru1I TTAA 6 cut(s) 1055, 1122, 1422, 2028, 2511, 2658
Tru9I TTAA 6 cut(s) 1055, 1122, 1422, 2028, 2511, 2658
TscAI CASTG 7 cut(s) 328, 627, 1161, 1365, 1825, 2509, 2524
TseI GCWGC 9 cut(s) 917, 1397, 1476, 1979, 2012, 2224, 2342, 2557, 2759
TspDTI ATGAA 8 cut(s) 163, 285, 839, 1218, 1752, 1928, 2229, 2450
TspGWI ACGGA 3 cut(s) 517, 570, 795
TspRI CASTG 7 cut(s) 328, 627, 1161, 1365, 1825, 2509, 2524
Tth111I GACNNNGTC 1 cut(s) 1744
Van91I CCANNNNNTGG 1 cut(s) 1567
VpaK11BI GGWCC 2 cut(s) 178, 2282
XapI RAATTY 5 cut(s) 170, 1206, 1468, 1563, 1720
XceI RCATGY 2 cut(s) 961, 2884
XhoI CTCGAG 1 cut(s) 1490
XmiI GTMKAC 2 cut(s) 561, 1271
XmnI GAANNNNTTC 2 cut(s) 1629, 2077
XspI CTAG 4 cut(s) 113, 227, 384, 2039
ZrmI AGTACT 1 cut(s) 556
Zsp2I ATGCAT 1 cut(s) 2797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.