Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0012476

Physical Location & Seq

Genomic Coordinates Reverse (-)

17699911 .. 17702054

Gene Structure 2144 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGACCATTACTCTAATTAAGTTAAAATTGAGGGACTATTGCGTGACCCAACCTACCCGCCACATAAACGGTTTCCTCCTCACTTGTCACTTCACCTTTGCTTTCTTAAATTCCACCAATCCTTCTCTCTTTCCTCCTCAAAACGCTGATACAATGGCGGCCAAAGAACCAGAGATTATCAGGGACAAGGACCAGATGAGGAGGTGGTCACGCGCCATGAGAGCCCAAGGCAAGACCATCGGATTAGTACCCACAATGGGCTACCTCCACGAAGGACACGTTTCCCTCATCCGAGAAGCCCGCACCCACGCCGACGTCCTCGCCGTCTCGATCTACGTGAACCCGGGTCAGTTCGCCCCCTCCGAAGACCTCTCCACATACCCGTCCGATCTCCACGGCGACATCCGGAAGCTCACACAAATTCCCGGCGGCGTCGACGTCGTCTTCAACCCCCACAACCTGTACGACTACGGAGGAAGCACGGACGCAAGCGCACGTGCGAGTAATGGGGCGGTTAAGAGCGGCGGCTGCGAAACGGTGTCGTGCTTGGAGGACAAGGGGGTGGGGCACGAGACGTGGGTTAGGGTCGAGAGGTTGGAGAAGGGGATGTGCGGGCGGAGCAGGCCGGTGTTTTTCAGAGGGGTTGCGACGGTTGTGAGCAAGTTGTTTAATATCGTGGAGCCTGATGTTGCGGTGTTTGGGAAGAAGGATTATCAACAATGGCGGATAATTCAGCGGATGGTTCGAGATCTCGATTTTTCCGTTAGAGTGATAGGTTCTGAAATAGTGCGAGAAGACGATGGCCTGGCAATGAGTTCACGCAATGTGCGCCTCTCACCTGAAGAACGGGAAAAGGCACTGTCTATAAGCAAGTCATTGTCAGAAGCTAGATCTTCTGCAGAAAAGGGACAAGTTAATTGTAAAGAGTTGAGAGCCTTGGTCATCCAAGCGATTAGTGAAGCCGGTGGAAGAGTCGATTATGCTGAGATTGTCGACCAAGAAAGCTTGGAGTCGGTAGAAGAGATCAGAAGGCCTGTTGTGTTCTGTGTCGCTGCCTGGTTTGGAAAGGTTCGACTGATCGACAACATGGAGATCAACATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004592 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006573 GO:0006575 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009081 GO:0009108 GO:0009110 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0015939 GO:0015940 GO:0016053 GO:0016874 GO:0016879 GO:0016881 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0033317 GO:0034641 GO:0042364 GO:0042398 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0061458 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605

KEGG Pathways

Metabolic & Signaling

ko00410 ko00770 ko01100 ko01110 map00410 map00770 map01100 map01110

Pfam Domains

Protein Families

Pantoate_ligase

Protein Analysis

368

Amino Acids

40.95

Weight (kDa)

7.08

Isoelectric Point (pI)

40.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pantoate_ligase	PF02569	59 - 363	1.8e-98	Pantoate-beta-alanine ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48840
fragaria_vesca	FvH4_2g19300
malus_domestica	MD10G1166100.v1.1
prunus_persica	Prupe.8G204600_v2.0.a1 Prupe.8G204600_v2.0.a1
pyrus_communis	pycom10g14330
rosa_chinensis	RchiOBHm_Chr6g0285101
rosa_laevigata	RLG00000012672
rosa_multiflora	Rmu_sc0002535.1_g000036 Rmu_sc0003797.1_g000011
rosa_roxburghii	Rroxscaffold_7G00183530 Rroxscaffold_7G00183670 Rroxscaffold_7G00184030
rosa_rugosa	Rorug06G0171100
rosa_samantha	Rh6AG282300 Rh6BG283000 Rh6CG284900 Rh6DG278000
rosa_wichuraiana	Rw6G024270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	318, 441
AccBSI	CCGCTC	1 cut(s)	522
AccI	GTMKAC	2 cut(s)	435, 993
AccII	CGCG	1 cut(s)	213
AccIII	TCCGGA	1 cut(s)	405
AcoI	YGGCCR	1 cut(s)	159
AcsI	RAATTY	2 cut(s)	109, 420
AcuI	CTGAAG	1 cut(s)	861
AcvI	CACGTG	1 cut(s)	497
AcyI	GRCGYC	3 cut(s)	315, 432, 438
AfaI	GTAC	2 cut(s)	249, 464
AfiI	CCNNNNNNNGG	1 cut(s)	257
AflIII	ACRYGT	1 cut(s)	277
AgsI	TTSAA	1 cut(s)	448
AjiI	CACGTC	1 cut(s)	576
AjnI	CCWGG	2 cut(s)	804, 1055
AluBI	AGCT	3 cut(s)	412, 887, 1005
AluI	AGCT	3 cut(s)	412, 887, 1005
Alw26I	GTCTC	2 cut(s)	331, 566
Ama87I	CYCGRG	1 cut(s)	343
Aor13HI	TCCGGA	1 cut(s)	405
AoxI	GGCC	4 cut(s)	159, 623, 802, 1031
ApeKI	GCWGC	2 cut(s)	528, 1052
ApoI	RAATTY	2 cut(s)	109, 420
AspLEI	GCGC	3 cut(s)	215, 494, 831
AspS9I	GGNCC	1 cut(s)	190
AsuC2I	CCSGG	3 cut(s)	344, 345, 426
AsuHPI	GGTGA	2 cut(s)	85, 828
AvaI	CYCGRG	1 cut(s)	343
AvaII	GGWCC	1 cut(s)	190
BaeGI	GKGCMC	1 cut(s)	570
BanII	GRGCYC	1 cut(s)	226
BauI	CACGAG	1 cut(s)	569
BbrPI	CACGTG	1 cut(s)	497
BbsI	GAAGAC	3 cut(s)	372, 436, 801
BbvI	GCAGC	2 cut(s)	515, 1039
BccI	CCATC	3 cut(s)	245, 733, 794
BceAI	ACGGC	2 cut(s)	308, 412
BcgI	CGANNNNNNTGC	2 cut(s)	220, 254
BciT130I	CCWGG	2 cut(s)	806, 1057
BcnI	CCSGG	3 cut(s)	344, 345, 426
BcoDI	GTCTC	2 cut(s)	331, 566
BfaI	CTAG	1 cut(s)	888
BfmI	CTRYAG	1 cut(s)	897
BglII	AGATCT	2 cut(s)	748, 890
BisI	GCNGC	6 cut(s)	159, 430, 523, 526, 529, 1053
BlsI	GCNGC	6 cut(s)	160, 431, 524, 527, 530, 1054
Bme1390I	CCNGG	5 cut(s)	344, 345, 426, 806, 1057
Bme18I	GGWCC	1 cut(s)	190
BmeT110I	CYCGRG	1 cut(s)	343
BmgBI	CACGTC	1 cut(s)	576
BmgT120I	GGNCC	1 cut(s)	190
BmiI	GGNNCC	1 cut(s)	681
BmrFI	CCNGG	5 cut(s)	344, 345, 426, 806, 1057
BpiI	GAAGAC	3 cut(s)	372, 436, 801
BpuMI	CCSGG	3 cut(s)	344, 345, 426
BsaAI	YACGTR	2 cut(s)	337, 497
BsaHI	GRCGYC	3 cut(s)	315, 432, 438
BsaJI	CCNNGG	4 cut(s)	226, 343, 394, 936
BsaWI	WCCGGW	1 cut(s)	405
Bsc4I	CCNNNNNNNGG	1 cut(s)	257
Bse118I	RCCGGY	2 cut(s)	625, 962
Bse3DI	GCAATG	2 cut(s)	816, 829
BseAI	TCCGGA	1 cut(s)	405
BseBI	CCWGG	2 cut(s)	806, 1057
BseDI	CCNNGG	4 cut(s)	226, 343, 394, 936
BseGI	GGATG	5 cut(s)	288, 402, 612, 744, 942
BseLI	CCNNNNNNNGG	1 cut(s)	257
BseMI	GCAATG	2 cut(s)	816, 829
BseMII	CTCAG	1 cut(s)	975
BseRI	GAGGAG	3 cut(s)	68, 126, 214
BseSI	GKGCMC	1 cut(s)	570
BseXI	GCAGC	2 cut(s)	515, 1039
Bsh1236I	CGCG	1 cut(s)	213
BshFI	GGCC	4 cut(s)	161, 625, 804, 1033
BsiHKCI	CYCGRG	1 cut(s)	343
BsiSI	CCGG	5 cut(s)	344, 406, 426, 626, 963
BslFI	GGGAC	3 cut(s)	47, 197, 921
BslI	CCNNNNNNNGG	1 cut(s)	257
BsmAI	GTCTC	2 cut(s)	331, 566
BsmBI	CGTCTC	2 cut(s)	331, 566
BsmFI	GGGAC	3 cut(s)	47, 197, 921
BsnI	GGCC	4 cut(s)	161, 625, 804, 1033
BsoBI	CYCGRG	1 cut(s)	343
Bsp1286I	GDGCHC	2 cut(s)	226, 570
Bsp13I	TCCGGA	1 cut(s)	405
Bsp143I	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
BspANI	GGCC	4 cut(s)	161, 625, 804, 1033
BspCNI	CTCAG	1 cut(s)	976
BspEI	TCCGGA	1 cut(s)	405
BspFNI	CGCG	1 cut(s)	213
BspLI	GGNNCC	1 cut(s)	681
BspMAI	CTGCAG	1 cut(s)	901
BsrBI	CCGCTC	1 cut(s)	522
BsrDI	GCAATG	2 cut(s)	816, 829
BsrFI	RCCGGY	2 cut(s)	625, 962
BssAI	RCCGGY	2 cut(s)	625, 962
BssECI	CCNNGG	4 cut(s)	226, 343, 394, 936
BssMI	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
BssNI	GRCGYC	3 cut(s)	315, 432, 438
BssSI	CACGAG	1 cut(s)	569
BssT1I	CCWWGG	2 cut(s)	226, 936
Bst2BI	CACGAG	1 cut(s)	569
Bst2UI	CCWGG	2 cut(s)	806, 1057
Bst4CI	ACNGT	4 cut(s)	71, 538, 652, 861
Bst6I	CTCTTC	2 cut(s)	964, 1014
BstACI	GRCGYC	3 cut(s)	315, 432, 438
BstBAI	YACGTR	2 cut(s)	337, 497
BstC8I	GCNNGC	4 cut(s)	301, 490, 614, 623
BstDEI	CTNAG	1 cut(s)	984
BstDSI	CCRYGG	1 cut(s)	394
BstF5I	GGATG	5 cut(s)	288, 402, 612, 744, 942
BstFNI	CGCG	1 cut(s)	213
BstHHI	GCGC	3 cut(s)	215, 494, 831
BstKTI	GATC	7 cut(s)	333, 391, 751, 893, 1026, 1080, 1095
BstMAI	GTCTC	2 cut(s)	331, 566
BstMBI	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
BstMWI	GCNNNNNNNGC	5 cut(s)	221, 528, 618, 622, 828
BstNI	CCWGG	2 cut(s)	806, 1057
BstSCI	CCNGG	5 cut(s)	342, 343, 424, 804, 1055
BstSFI	CTRYAG	1 cut(s)	897
BstSLI	GKGCMC	1 cut(s)	570
BstUI	CGCG	1 cut(s)	213
BstV1I	GCAGC	2 cut(s)	515, 1039
BstV2I	GAAGAC	3 cut(s)	372, 436, 801
BstX2I	RGATCY	2 cut(s)	748, 890
BstYI	RGATCY	2 cut(s)	748, 890
BsuRI	GGCC	4 cut(s)	161, 625, 804, 1033
BtgI	CCRYGG	1 cut(s)	394
BtrI	CACGTC	1 cut(s)	576
BtsCI	GGATG	5 cut(s)	288, 402, 612, 744, 942
BtsIMutI	CAGTG	1 cut(s)	857
Cac8I	GCNNGC	4 cut(s)	301, 490, 614, 623
CfoI	GCGC	3 cut(s)	215, 494, 831
Cfr10I	RCCGGY	2 cut(s)	625, 962
Cfr13I	GGNCC	1 cut(s)	190
Cfr9I	CCCGGG	1 cut(s)	343
CseI	GACGC	2 cut(s)	421, 494
Csp6I	GTAC	2 cut(s)	248, 463
CviAII	CATG	2 cut(s)	217, 1087
CviQI	GTAC	2 cut(s)	248, 463
DdeI	CTNAG	1 cut(s)	984
DpnI	GATC	7 cut(s)	332, 390, 750, 892, 1025, 1079, 1094
DpnII	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
EaeI	YGGCCR	1 cut(s)	159
Eam1104I	CTCTTC	2 cut(s)	964, 1014
EarI	CTCTTC	2 cut(s)	964, 1014
EciI	GGCGGA	2 cut(s)	631, 739
Eco130I	CCWWGG	2 cut(s)	226, 936
Eco147I	AGGCCT	1 cut(s)	1033
Eco24I	GRGCYC	1 cut(s)	226
Eco47I	GGWCC	1 cut(s)	190
Eco57I	CTGAAG	1 cut(s)	861
Eco72I	CACGTG	1 cut(s)	497
Eco88I	CYCGRG	1 cut(s)	343
EcoRII	CCWGG	2 cut(s)	804, 1055
EcoT14I	CCWWGG	2 cut(s)	226, 936
EcoT38I	GRGCYC	1 cut(s)	226
ErhI	CCWWGG	2 cut(s)	226, 936
Esp3I	CGTCTC	2 cut(s)	331, 566
FaeI	CATG	2 cut(s)	220, 1090
FaiI	YATR	8 cut(s)	65, 218, 379, 866, 981, 1088, 1100, 1102
FaqI	GGGAC	3 cut(s)	47, 197, 921
FatI	CATG	2 cut(s)	216, 1086
FauI	CCCGC	3 cut(s)	65, 308, 605
FauNDI	CATATG	1 cut(s)	1100
FblI	GTMKAC	2 cut(s)	435, 993
Fnu4HI	GCNGC	6 cut(s)	159, 430, 523, 526, 529, 1053
FokI	GGATG	5 cut(s)	275, 389, 619, 751, 929
FriOI	GRGCYC	1 cut(s)	226
Fsp4HI	GCNGC	6 cut(s)	159, 430, 523, 526, 529, 1053
FspBI	CTAG	1 cut(s)	888
GlaI	GCGC	3 cut(s)	214, 493, 830
GluI	GCNGC	6 cut(s)	159, 430, 523, 526, 529, 1053
HaeIII	GGCC	4 cut(s)	161, 625, 804, 1033
HapII	CCGG	5 cut(s)	344, 406, 426, 626, 963
HgaI	GACGC	2 cut(s)	421, 494
HhaI	GCGC	3 cut(s)	215, 494, 831
Hin1I	GRCGYC	3 cut(s)	315, 432, 438
Hin1II	CATG	2 cut(s)	220, 1090
Hin6I	GCGC	3 cut(s)	213, 492, 829
HinP1I	GCGC	3 cut(s)	213, 492, 829
HincII	GTYRAC	2 cut(s)	436, 994
HindII	GTYRAC	2 cut(s)	436, 994
HindIII	AAGCTT	1 cut(s)	1003
HinfI	GANTC	2 cut(s)	972, 1010
HpaII	CCGG	5 cut(s)	344, 406, 426, 626, 963
HphI	GGTGA	2 cut(s)	85, 828
Hpy166II	GTNNAC	4 cut(s)	340, 436, 818, 994
Hpy188I	TCNGA	8 cut(s)	242, 293, 364, 388, 638, 781, 883, 1028
Hpy188III	TCNNGA	5 cut(s)	328, 406, 589, 746, 752
Hpy8I	GTNNAC	4 cut(s)	340, 436, 818, 994
Hpy99I	CGWCG	5 cut(s)	317, 437, 440, 443, 652
HpyAV	CCTTC	5 cut(s)	132, 266, 595, 700, 1023
HpyCH4III	ACNGT	4 cut(s)	71, 538, 652, 861
HpyCH4IV	ACGT	6 cut(s)	279, 315, 336, 438, 496, 575
HpyCH4V	TGCA	1 cut(s)	899
HpyF10VI	GCNNNNNNNGC	5 cut(s)	221, 528, 618, 622, 828
HpyF3I	CTNAG	1 cut(s)	984
HpySE526I	ACGT	6 cut(s)	279, 315, 336, 438, 496, 575
Hsp92I	GRCGYC	3 cut(s)	315, 432, 438
Hsp92II	CATG	2 cut(s)	220, 1090
HspAI	GCGC	3 cut(s)	213, 492, 829
Kpn2I	TCCGGA	1 cut(s)	405
Kzo9I	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
LmnI	GCTCC	2 cut(s)	618, 679
Lsp1109I	GCAGC	2 cut(s)	515, 1039
MaeI	CTAG	1 cut(s)	888
MaeII	ACGT	6 cut(s)	279, 315, 336, 438, 496, 575
MaeIII	GTNAC	3 cut(s)	43, 86, 207
MalI	GATC	7 cut(s)	332, 390, 750, 892, 1025, 1079, 1094
MbiI	CCGCTC	1 cut(s)	522
MboI	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
MboII	GAAGA	8 cut(s)	377, 436, 715, 806, 854, 885, 981, 1031
MflI	RGATCY	2 cut(s)	748, 890
MhlI	GDGCHC	2 cut(s)	226, 570
MluCI	AATT	6 cut(s)	15, 26, 109, 420, 729, 916
MlyI	GAGTC	2 cut(s)	981, 1019
MmeI	TCCRAC	1 cut(s)	576
MroI	TCCGGA	1 cut(s)	405
MseI	TTAA	6 cut(s)	18, 23, 107, 516, 669, 915
MspA1I	CMGCKG	1 cut(s)	736
MspI	CCGG	5 cut(s)	344, 406, 426, 626, 963
MspR9I	CCNGG	5 cut(s)	344, 345, 426, 806, 1057
MvaI	CCWGG	2 cut(s)	806, 1057
MvnI	CGCG	1 cut(s)	213
MwoI	GCNNNNNNNGC	5 cut(s)	221, 528, 618, 622, 828
NciI	CCSGG	3 cut(s)	344, 345, 426
NdeI	CATATG	1 cut(s)	1100
NdeII	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
NlaIII	CATG	2 cut(s)	220, 1090
NlaIV	GGNNCC	1 cut(s)	681
NmuCI	GTSAC	3 cut(s)	43, 86, 207
PceI	AGGCCT	1 cut(s)	1033
PcsI	WCGNNNNNNNCGW	4 cut(s)	276, 321, 360, 759
PflFI	GACNNNGTC	1 cut(s)	440
PkrI	GCNGC	6 cut(s)	160, 431, 524, 527, 530, 1054
PleI	GAGTC	2 cut(s)	980, 1018
PmaCI	CACGTG	1 cut(s)	497
PmlI	CACGTG	1 cut(s)	497
PpsI	GAGTC	2 cut(s)	980, 1018
Ppu21I	YACGTR	2 cut(s)	337, 497
Psp6I	CCWGG	2 cut(s)	804, 1055
PspCI	CACGTG	1 cut(s)	497
PspGI	CCWGG	2 cut(s)	804, 1055
PspN4I	GGNNCC	1 cut(s)	681
PspPI	GGNCC	1 cut(s)	190
PstI	CTGCAG	1 cut(s)	901
PsuI	RGATCY	2 cut(s)	748, 890
PsyI	GACNNNGTC	1 cut(s)	440
RsaI	GTAC	2 cut(s)	249, 464
RsaNI	GTAC	2 cut(s)	248, 463
SalI	GTCGAC	2 cut(s)	434, 992
SaqAI	TTAA	6 cut(s)	18, 23, 107, 516, 669, 915
SatI	GCNGC	6 cut(s)	159, 430, 523, 526, 529, 1053
Sau3AI	GATC	7 cut(s)	330, 388, 748, 890, 1023, 1077, 1092
Sau96I	GGNCC	1 cut(s)	190
SchI	GAGTC	2 cut(s)	981, 1019
ScrFI	CCNGG	5 cut(s)	344, 345, 426, 806, 1057
SduI	GDGCHC	2 cut(s)	226, 570
SfcI	CTRYAG	1 cut(s)	897
SgrDI	CGTCGACG	1 cut(s)	434
SinI	GGWCC	1 cut(s)	190
SmaI	CCCGGG	1 cut(s)	345
Sse9I	AATT	6 cut(s)	15, 26, 109, 420, 729, 916
SseBI	AGGCCT	1 cut(s)	1033
SspMI	CTAG	1 cut(s)	888
StuI	AGGCCT	1 cut(s)	1033
StyD4I	CCNGG	5 cut(s)	342, 343, 424, 804, 1055
StyI	CCWWGG	2 cut(s)	226, 936
TaaI	ACNGT	4 cut(s)	71, 538, 652, 861
TaiI	ACGT	6 cut(s)	282, 318, 339, 441, 499, 578
TaqI	TCGA	9 cut(s)	329, 435, 588, 745, 753, 975, 993, 1072, 1080
TasI	AATT	6 cut(s)	15, 26, 109, 420, 729, 916
TauI	GCSGC	4 cut(s)	161, 432, 525, 528
Tru1I	TTAA	6 cut(s)	18, 23, 107, 516, 669, 915
Tru9I	TTAA	6 cut(s)	18, 23, 107, 516, 669, 915
TscAI	CASTG	1 cut(s)	864
TseFI	GTSAC	3 cut(s)	43, 86, 207
TseI	GCWGC	2 cut(s)	528, 1052
Tsp45I	GTSAC	3 cut(s)	43, 86, 207
TspGWI	ACGGA	3 cut(s)	486, 497, 751
TspMI	CCCGGG	1 cut(s)	343
TspRI	CASTG	1 cut(s)	864
Tth111I	GACNNNGTC	1 cut(s)	440
VpaK11BI	GGWCC	1 cut(s)	190
XapI	RAATTY	2 cut(s)	109, 420
XmaI	CCCGGG	1 cut(s)	343
XmiI	GTMKAC	2 cut(s)	435, 993
XspI	CTAG	1 cut(s)	888
ZraI	GACGTC	2 cut(s)	316, 439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.