Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0013873

Physical Location & Seq

Genomic Coordinates Reverse (-)

19996312 .. 19998165

Gene Structure 1854 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGACCATGCCTTCCACGGCAACAGCGGCAGCTACAGGTACACGGGTTAAGCCTGTTACTGGTACTACAAGACACCGCCGGCTTCCTTCTTTGAACTCTTCAAATTCCTTCAGAAATCAATTGGGATTTTCCCCCTCTTCTTCCTCAATTTTCAAATCAAAAACCATCTCTTGCTCTCTGCAAACACCTTCTCTAGTCCAAATTCCCAAACACTCCCCTACCTACCAACAACCTTCAACTTCAACTTCCACTAAAATCCCAAGAGAAAATAAGACCCAAAAGTCCCCAACTTCTCCAAAGCCAGTGCCACAACAATGGAACTTGTTACAGAGAGCAGCATCCATGGCTTTGGACGCAATGGAGAGCGCTCTGCTTTCCAAGGAAAAACAACACCCACTTCCCAAAACATCCGACCCAGAAGTCCAAATCGCCGGAAATTTTGCTCCGGTGCCGGAGCACCCAGTCGAACACTATCTACCGGTGACGGGAAAAATCCCCGACTCAATCCAAGGCCTCTACGTCCGAAACGGCGCCAACCCACTTCACGAGCCGGTCGCCGGTCACCACCTCTTCGACGGCGACGGCATGGTCCACGCCCTCCAGTTCAAAGACGGAACCGCCAGCTACGCTTGCAGGTTCACCGAAACCAGCCGTATCAGCCAAGAACGTGACCTCGGCCGTCCCCTGTTTCCCAAAGCCATTGGCGAGCTCCACGGCCACTCCGGCATCGCCCGCCTCCTCCTTTTCTACGCCAGAGGGATGTTCGGACTCGTGGACCCCACCCACGGCGTTGGCGTCGCGAACGCCGGCCTCGTCTACCACAACGGTCACCTCCTCGCCATGTCGGAAGACGACTTGCCGTATCACGTCCAAATCACCGAAACAGGGGACCTCAAAACGGTCGGCCGCTACGACTTCGAAGGGCAGCTGAAGTCCACAATGATCGCCCACCCGAAGGTTGACCCCGCCACGGGGGAGCTCTTCGCGCTCAGCTACGACGTCGTTCAGAGGCCTTACTTGAAATACTTCAAATTCTCCCCAAACGGCGAGAAGTCCCCCGACATCGAAATCAACCTCGAGCAGCCCACCATGATGCACGACTTCGCCATTACCGAACGACACGTCGTAATCCCCGACCAGCAGGTGGTGTTCAAGCTGCAGGAGATGATCGGCGGTGGGTCCCCCGTGATTTACGACAAGAACAAGATGTCGCGTTTTGGGATCCTCGACAAGAGTGCCAAGGACGCGTCTGGAATCCGGTGGGTCGACTGCCCCGACTGCTTCTGCTTCCACCTCTGGAACGCGTGGGAGGAGCCGGAGACGGACGAGATCGTCGTTATCGGCTCGTGCATGACGCCCCCGGACTCCATTTTCAATGAATGCGACGAGAATTTAGAGAGTGTTCTGTCCGAAATCCGGCTCAATTTGAAAACCGGCAAGTCGACGCGCCGCCGCATTTGCTCCGAGAATCTGAACCTGGAAGCTGGGATGGTGAATCGGAACAAGCTAGGGAGAAAAACCCAGTTCGCGTATTTGGCCCTGGCGGATCCGTGGCCGAAAGTTTCGGGTTTTGCGAAGGTGGACCTCTCCACTGGTGAGGTGAAGAAGCATATCTACGGTGACGAGAAGTACGGTGGCGAACCTCTGTTTCTTCCCAGAGACCCCAACTCGGAGAACGAGGACGATGGTTACATTTTGGCCTTCGTTCACGACGAGAAGAAGTGGAAGTCGGAGCTGCAAATCGTGAACGCCATGGATTTGAAGGTGGAAGCTACAATTCAGCTGCCCTCGAGAGTTCCGTACGGTTTTCATGGAACGTTTATAAGCGCTAAGGACTTGAAGAAGCAGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0006970 GO:0006972 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009651 GO:0009687 GO:0009688 GO:0009791 GO:0009987 GO:0010035 GO:0010154 GO:0010162 GO:0010431 GO:0010436 GO:0016020 GO:0016053 GO:0016106 GO:0016114 GO:0016491 GO:0016701 GO:0016702 GO:0019752 GO:0021700 GO:0022414 GO:0022611 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034357 GO:0042221 GO:0042538 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045549 GO:0046165 GO:0046394 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051213 GO:0055035 GO:0055114 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

RPE65

Protein Analysis

618

Amino Acids

68.5

Weight (kDa)

6.75

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	148 - 609	2.3e-130	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0013873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78390
fragaria_vesca	FvH4_3g16730
malus_domestica	MD05G1207300.v1.1 MD10G1194200.v1.1
prunus_persica	Prupe.4G150100_v2.0.a1
pyrus_communis	pycom05g19340 pycom10g16810
rosa_chinensis	RchiOBHm_Chr5g0027901
rosa_laevigata	RLG00000033052
rosa_multiflora	Rmu_sc0000309.1_g000029
rosa_roxburghii	Rroxscaffold_1G00051730
rosa_rugosa	Rorug05G0102400.1
rosa_samantha	Rh5AG196700 Rh5BG194600 Rh5CG214600 Rh5DG196300
rosa_wichuraiana	Rw5G017900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1823
AarI	CACCTGC	1 cut(s)	1132
AasI	GACNNNNNNGTC	4 cut(s)	280, 419, 587, 1331
AatII	GACGTC	1 cut(s)	1002
Acc36I	ACCTGC	2 cut(s)	624, 1132
AccB1I	GGYRCC	2 cut(s)	448, 530
AccB7I	CCANNNNNTGG	1 cut(s)	1144
AccI	GTMKAC	3 cut(s)	816, 1266, 1442
AccII	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
AclI	AACGTT	1 cut(s)	1817
AclWI	GGATC	4 cut(s)	1216, 1229, 1541, 1554
AcoI	YGGCCR	4 cut(s)	676, 715, 904, 1553
AcsI	RAATTY	5 cut(s)	103, 201, 436, 1031, 1390
AcuI	CTGAAG	2 cut(s)	94, 950
AcyI	GRCGYC	4 cut(s)	531, 795, 999, 1355
AfaI	GTAC	4 cut(s)	40, 64, 1631, 1802
AfeI	AGCGCT	2 cut(s)	367, 1828
AflIII	ACRYGT	3 cut(s)	1120, 1245, 1302
AgeI	ACCGGT	1 cut(s)	478
AjiI	CACGTC	2 cut(s)	868, 1123
AjnI	CCWGG	2 cut(s)	1476, 1539
Alw21I	GWGCWC	3 cut(s)	459, 711, 981
Alw26I	GTCTC	2 cut(s)	1313, 1653
AlwI	GGATC	4 cut(s)	1216, 1229, 1541, 1554
Ama87I	CYCGRG	2 cut(s)	1076, 1789
Aor51HI	AGCGCT	2 cut(s)	367, 1828
ApeKI	GCWGC	7 cut(s)	29, 335, 925, 1081, 1156, 1735, 1783
ApoI	RAATTY	5 cut(s)	103, 201, 436, 1031, 1390
ArsI	GACNNNNNNTTYG	2 cut(s)	1107, 1139
AsiGI	ACCGGT	1 cut(s)	478
Asp700I	GAANNNNTTC	1 cut(s)	1025
AspLEI	GCGC	5 cut(s)	368, 533, 988, 1449, 1829
AspS9I	GGNCC	6 cut(s)	589, 775, 889, 1179, 1537, 1582
AsuC2I	CCSGG	1 cut(s)	1361
AsuHPI	GGTGA	9 cut(s)	493, 554, 631, 821, 868, 1504, 1607, 1612, 1631
AsuII	TTCGAA	1 cut(s)	918
AvaI	CYCGRG	2 cut(s)	1076, 1789
AvaII	GGWCC	5 cut(s)	589, 775, 889, 1179, 1582
BaeI	ACNNNNGTAYC	2 cut(s)	30, 63
BamHI	GGATCC	2 cut(s)	1221, 1546
BanI	GGYRCC	2 cut(s)	448, 530
BanII	GRGCYC	2 cut(s)	711, 981
BarI	GAAGNNNNNNTAC	2 cut(s)	1598, 1630
BauI	CACGAG	3 cut(s)	545, 770, 1345
BbsI	GAAGAC	1 cut(s)	855
Bbv12I	GWGCWC	3 cut(s)	459, 711, 981
BbvI	GCAGC	7 cut(s)	41, 347, 937, 1093, 1143, 1722, 1770
BccI	CCATC	3 cut(s)	173, 1483, 1679
BcgI	CGANNNNNNTGC	2 cut(s)	1217, 1251
BciT130I	CCWGG	2 cut(s)	1478, 1541
BcnI	CCSGG	1 cut(s)	1361
BcoDI	GTCTC	2 cut(s)	1313, 1653
BfaI	CTAG	2 cut(s)	194, 1508
BfmI	CTRYAG	2 cut(s)	33, 1157
BfoI	RGCGCY	3 cut(s)	369, 534, 1830
BfuAI	ACCTGC	2 cut(s)	624, 1132
BglI	GCCNNNNNGGC	1 cut(s)	723
BlpI	GCTNAGC	1 cut(s)	989
Bme1390I	CCNGG	3 cut(s)	1361, 1478, 1541
Bme18I	GGWCC	5 cut(s)	589, 775, 889, 1179, 1582
BmeT110I	CYCGRG	2 cut(s)	1076, 1789
BmgBI	CACGTC	2 cut(s)	868, 1123
BmgT120I	GGNCC	6 cut(s)	589, 775, 889, 1179, 1537, 1582
BmrFI	CCNGG	3 cut(s)	1361, 1478, 1541
BmrI	ACTGGG	2 cut(s)	455, 1516
BmsI	GCATC	3 cut(s)	347, 735, 1083
BmuI	ACTGGG	2 cut(s)	455, 1516
BpiI	GAAGAC	1 cut(s)	855
BpmI	CTGGAG	1 cut(s)	584
Bpu10I	CCTNAGC	1 cut(s)	1830
Bpu1102I	GCTNAGC	1 cut(s)	989
Bpu14I	TTCGAA	1 cut(s)	918
BpuMI	CCSGG	1 cut(s)	1361
BsaHI	GRCGYC	4 cut(s)	531, 795, 999, 1355
BsaI	GGTCTC	1 cut(s)	1653
BsaWI	WCCGGW	3 cut(s)	445, 478, 1257
BsaXI	ACNNNNNCTCC	2 cut(s)	704, 734
Bse118I	RCCGGY	6 cut(s)	78, 478, 550, 557, 806, 1433
Bse1I	ACTGG	6 cut(s)	64, 302, 461, 601, 1522, 1597
Bse3DI	GCAATG	1 cut(s)	363
BseBI	CCWGG	2 cut(s)	1478, 1541
BseGI	GGATG	4 cut(s)	338, 407, 765, 1494
BseMI	GCAATG	1 cut(s)	363
BseMII	CTCAG	1 cut(s)	1003
BseNI	ACTGG	6 cut(s)	64, 302, 461, 601, 1522, 1597
BseRI	GAGGAG	3 cut(s)	728, 824, 1325
BseX3I	CGGCCG	2 cut(s)	676, 904
BseXI	GCAGC	7 cut(s)	41, 347, 937, 1093, 1143, 1722, 1770
BseYI	CCCAGC	1 cut(s)	1484
Bsh1236I	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
Bsh1285I	CGRYCG	4 cut(s)	555, 679, 903, 907
BshNI	GGYRCC	2 cut(s)	448, 530
BshTI	ACCGGT	1 cut(s)	478
BsiEI	CGRYCG	4 cut(s)	555, 679, 903, 907
BsiHKAI	GWGCWC	3 cut(s)	459, 711, 981
BsiHKCI	CYCGRG	2 cut(s)	1076, 1789
BsiWI	CGTACG	1 cut(s)	1800
BslFI	GGGAC	5 cut(s)	268, 666, 902, 1039, 1165
BsmAI	GTCTC	2 cut(s)	1313, 1653
BsmBI	CGTCTC	1 cut(s)	1313
BsmFI	GGGAC	5 cut(s)	268, 666, 902, 1039, 1165
BsmI	GAATGC	1 cut(s)	1385
Bso31I	GGTCTC	1 cut(s)	1653
BsoBI	CYCGRG	2 cut(s)	1076, 1789
Bsp119I	TTCGAA	1 cut(s)	918
Bsp1286I	GDGCHC	3 cut(s)	459, 711, 981
Bsp143I	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
Bsp1720I	GCTNAGC	1 cut(s)	989
Bsp19I	CCATGG	2 cut(s)	342, 1752
Bsp68I	TCGCGA	1 cut(s)	800
BspCNI	CTCAG	1 cut(s)	1002
BspFNI	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
BspMAI	CTGCAG	1 cut(s)	1161
BspMI	ACCTGC	2 cut(s)	624, 1132
BspPI	GGATC	4 cut(s)	1216, 1229, 1541, 1554
BspQI	GCTCTTC	1 cut(s)	986
BspT104I	TTCGAA	1 cut(s)	918
BspT107I	GGYRCC	2 cut(s)	448, 530
BspTNI	GGTCTC	1 cut(s)	1653
BsrDI	GCAATG	1 cut(s)	363
BsrFI	RCCGGY	6 cut(s)	78, 478, 550, 557, 806, 1433
BsrI	ACTGG	6 cut(s)	64, 302, 461, 601, 1522, 1597
BssAI	RCCGGY	6 cut(s)	78, 478, 550, 557, 806, 1433
BssMI	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
BssNI	GRCGYC	4 cut(s)	531, 795, 999, 1355
BssSI	CACGAG	3 cut(s)	545, 770, 1345
BssT1I	CCWWGG	5 cut(s)	342, 378, 508, 1239, 1752
Bst2BI	CACGAG	3 cut(s)	545, 770, 1345
Bst2UI	CCWGG	2 cut(s)	1478, 1541
Bst4CI	ACNGT	5 cut(s)	827, 901, 1619, 1634, 1805
Bst6I	CTCTTC	4 cut(s)	103, 142, 575, 986
BstACI	GRCGYC	4 cut(s)	531, 795, 999, 1355
BstBI	TTCGAA	1 cut(s)	918
BstC8I	GCNNGC	7 cut(s)	80, 622, 631, 707, 733, 808, 1848
BstDEI	CTNAG	2 cut(s)	989, 1830
BstDSI	CCRYGG	7 cut(s)	15, 342, 712, 784, 969, 1550, 1752
BstEII	GGTNACC	2 cut(s)	560, 827
BstF5I	GGATG	4 cut(s)	338, 407, 765, 1494
BstFNI	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
BstH2I	RGCGCY	3 cut(s)	369, 534, 1830
BstHHI	GCGC	5 cut(s)	368, 533, 988, 1449, 1829
BstKTI	GATC	5 cut(s)	945, 1170, 1224, 1332, 1549
BstMAI	GTCTC	2 cut(s)	1313, 1653
BstMBI	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
BstMCI	CGRYCG	4 cut(s)	555, 679, 903, 907
BstNI	CCWGG	2 cut(s)	1478, 1541
BstPI	GGTNACC	2 cut(s)	560, 827
BstSCI	CCNGG	3 cut(s)	1359, 1476, 1539
BstSFI	CTRYAG	2 cut(s)	33, 1157
BstUI	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
BstV1I	GCAGC	7 cut(s)	41, 347, 937, 1093, 1143, 1722, 1770
BstV2I	GAAGAC	1 cut(s)	855
BstX2I	RGATCY	2 cut(s)	1221, 1546
BstXI	CCANNNNNNTGG	3 cut(s)	315, 349, 791
BstYI	RGATCY	2 cut(s)	1221, 1546
BstZI	CGGCCG	2 cut(s)	676, 904
BtgI	CCRYGG	7 cut(s)	15, 342, 712, 784, 969, 1550, 1752
BtgZI	GCGATG	1 cut(s)	712
BtrI	CACGTC	2 cut(s)	868, 1123
BtsCI	GGATG	4 cut(s)	338, 407, 765, 1494
BtsIMutI	CAGTG	2 cut(s)	309, 1590
BtuMI	TCGCGA	1 cut(s)	800
BveI	ACCTGC	2 cut(s)	624, 1132
Cac8I	GCNNGC	7 cut(s)	80, 622, 631, 707, 733, 808, 1848
CfoI	GCGC	5 cut(s)	368, 533, 988, 1449, 1829
Cfr10I	RCCGGY	6 cut(s)	78, 478, 550, 557, 806, 1433
Cfr13I	GGNCC	6 cut(s)	589, 775, 889, 1179, 1537, 1582
CseI	GACGC	6 cut(s)	362, 784, 1236, 1253, 1363, 1453
Csp6I	GTAC	4 cut(s)	39, 63, 1630, 1801
CspAI	ACCGGT	1 cut(s)	478
CviAII	CATG	8 cut(s)	7, 343, 586, 841, 1090, 1351, 1753, 1811
CviQI	GTAC	4 cut(s)	39, 63, 1630, 1801
DdeI	CTNAG	2 cut(s)	989, 1830
DinI	GGCGCC	1 cut(s)	532
DpnI	GATC	5 cut(s)	944, 1169, 1223, 1331, 1548
DpnII	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
DrdI	GACNNNNNNGTC	4 cut(s)	280, 419, 587, 1331
DseDI	GACNNNNNNGTC	4 cut(s)	280, 419, 587, 1331
EaeI	YGGCCR	4 cut(s)	676, 715, 904, 1553
EagI	CGGCCG	2 cut(s)	676, 904
Eam1104I	CTCTTC	4 cut(s)	103, 142, 575, 986
EarI	CTCTTC	4 cut(s)	103, 142, 575, 986
EciI	GGCGGA	1 cut(s)	1559
Ecl136II	GAGCTC	2 cut(s)	709, 979
EclXI	CGGCCG	2 cut(s)	676, 904
Eco130I	CCWWGG	5 cut(s)	342, 378, 508, 1239, 1752
Eco147I	AGGCCT	3 cut(s)	513, 1012, 1850
Eco24I	GRGCYC	2 cut(s)	711, 981
Eco31I	GGTCTC	1 cut(s)	1653
Eco47I	GGWCC	5 cut(s)	589, 775, 889, 1179, 1582
Eco47III	AGCGCT	2 cut(s)	367, 1828
Eco52I	CGGCCG	2 cut(s)	676, 904
Eco53kI	GAGCTC	2 cut(s)	709, 979
Eco57I	CTGAAG	2 cut(s)	94, 950
Eco88I	CYCGRG	2 cut(s)	1076, 1789
Eco91I	GGTNACC	2 cut(s)	560, 827
EcoICRI	GAGCTC	2 cut(s)	709, 979
EcoO109I	RGGNCCY	2 cut(s)	889, 1179
EcoO65I	GGTNACC	2 cut(s)	560, 827
EcoRII	CCWGG	2 cut(s)	1476, 1539
EcoT14I	CCWWGG	5 cut(s)	342, 378, 508, 1239, 1752
EcoT38I	GRGCYC	2 cut(s)	711, 981
EgeI	GGCGCC	1 cut(s)	532
EheI	GGCGCC	1 cut(s)	532
ErhI	CCWWGG	5 cut(s)	342, 378, 508, 1239, 1752
Esp3I	CGTCTC	1 cut(s)	1313
FaeI	CATG	8 cut(s)	10, 346, 589, 844, 1093, 1354, 1756, 1814
FaqI	GGGAC	5 cut(s)	268, 666, 902, 1039, 1165
FatI	CATG	8 cut(s)	6, 342, 585, 840, 1089, 1350, 1752, 1810
FauI	CCCGC	2 cut(s)	740, 973
FblI	GTMKAC	3 cut(s)	816, 1266, 1442
FokI	GGATG	4 cut(s)	325, 394, 772, 1501
FriOI	GRGCYC	2 cut(s)	711, 981
FspBI	CTAG	2 cut(s)	194, 1508
GlaI	GCGC	5 cut(s)	367, 532, 987, 1448, 1828
GsaI	CCCAGC	1 cut(s)	1488
GsuI	CTGGAG	1 cut(s)	584
HaeII	RGCGCY	3 cut(s)	369, 534, 1830
HgaI	GACGC	6 cut(s)	362, 784, 1236, 1253, 1363, 1453
HhaI	GCGC	5 cut(s)	368, 533, 988, 1449, 1829
Hin1I	GRCGYC	4 cut(s)	531, 795, 999, 1355
Hin1II	CATG	8 cut(s)	10, 346, 589, 844, 1093, 1354, 1756, 1814
Hin6I	GCGC	5 cut(s)	366, 531, 986, 1447, 1827
HinP1I	GCGC	5 cut(s)	366, 531, 986, 1447, 1827
HincII	GTYRAC	3 cut(s)	961, 1267, 1443
HindII	GTYRAC	3 cut(s)	961, 1267, 1443
HinfI	GANTC	6 cut(s)	500, 768, 1254, 1364, 1468, 1495
HphI	GGTGA	9 cut(s)	493, 554, 631, 821, 868, 1504, 1607, 1612, 1631
Hpy188III	TCNNGA	7 cut(s)	545, 799, 1251, 1297, 1709, 1744, 1791
HpyCH4III	ACNGT	5 cut(s)	827, 901, 1619, 1634, 1805
HpyCH4IV	ACGT	6 cut(s)	519, 667, 867, 999, 1122, 1817
HpyCH4V	TGCA	6 cut(s)	181, 633, 1096, 1159, 1350, 1738
HpyF3I	CTNAG	2 cut(s)	989, 1830
HpySE526I	ACGT	6 cut(s)	519, 667, 867, 999, 1122, 1817
Hsp92I	GRCGYC	4 cut(s)	531, 795, 999, 1355
Hsp92II	CATG	8 cut(s)	10, 346, 589, 844, 1093, 1354, 1756, 1814
HspAI	GCGC	5 cut(s)	366, 531, 986, 1447, 1827
KasI	GGCGCC	1 cut(s)	530
KflI	GGGWCCC	1 cut(s)	1179
KroI	GCCGGC	2 cut(s)	78, 806
KroNI	GCCGGC	2 cut(s)	80, 808
Kzo9I	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
LguI	GCTCTTC	1 cut(s)	986
LmnI	GCTCC	7 cut(s)	448, 454, 714, 976, 1312, 1466, 1732
Lsp1109I	GCAGC	7 cut(s)	41, 347, 937, 1093, 1143, 1722, 1770
LweI	GCATC	3 cut(s)	347, 735, 1083
MaeI	CTAG	2 cut(s)	194, 1508
MaeII	ACGT	6 cut(s)	519, 667, 867, 999, 1122, 1817
MaeIII	GTNAC	8 cut(s)	55, 324, 481, 560, 668, 827, 1619, 1688
MalI	GATC	5 cut(s)	944, 1169, 1223, 1331, 1548
MboI	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
MfeI	CAATTG	1 cut(s)	119
MflI	RGATCY	2 cut(s)	1221, 1546
MhlI	GDGCHC	3 cut(s)	459, 711, 981
MluCI	AATT	9 cut(s)	103, 119, 147, 201, 436, 1031, 1390, 1423, 1776
MluI	ACGCGT	2 cut(s)	1245, 1302
Mly113I	GGCGCC	1 cut(s)	531
MlyI	GAGTC	3 cut(s)	494, 762, 1358
MmeI	TCCRAC	3 cut(s)	435, 825, 1710
MroNI	GCCGGC	2 cut(s)	78, 806
MroXI	GAANNNNTTC	1 cut(s)	1025
MseI	TTAA	1 cut(s)	48
MslI	CAYNNNNRTG	2 cut(s)	313, 1091
MspA1I	CMGCKG	3 cut(s)	26, 928, 1783
MspR9I	CCNGG	3 cut(s)	1361, 1478, 1541
MunI	CAATTG	1 cut(s)	119
Mva1269I	GAATGC	1 cut(s)	1385
MvaI	CCWGG	2 cut(s)	1478, 1541
MvnI	CGCG	7 cut(s)	800, 986, 1213, 1247, 1304, 1447, 1529
NaeI	GCCGGC	2 cut(s)	80, 808
NarI	GGCGCC	1 cut(s)	531
NciI	CCSGG	1 cut(s)	1361
NcoI	CCATGG	2 cut(s)	342, 1752
NdeII	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
NgoMIV	GCCGGC	2 cut(s)	78, 806
NlaIII	CATG	8 cut(s)	10, 346, 589, 844, 1093, 1354, 1756, 1814
NmeAIII	GCCGAG	1 cut(s)	654
NmuCI	GTSAC	5 cut(s)	481, 560, 668, 827, 1619
NruI	TCGCGA	1 cut(s)	800
NspV	TTCGAA	1 cut(s)	918
PaeR7I	CTCGAG	2 cut(s)	1076, 1789
PaqCI	CACCTGC	1 cut(s)	1132
PceI	AGGCCT	3 cut(s)	513, 1012, 1850
PciSI	GCTCTTC	1 cut(s)	986
PctI	GAATGC	1 cut(s)	1385
PdiI	GCCGGC	2 cut(s)	80, 808
PdmI	GAANNNNTTC	1 cut(s)	1025
PfeI	GAWTC	3 cut(s)	1254, 1468, 1495
Pfl23II	CGTACG	1 cut(s)	1800
PflMI	CCANNNNNTGG	1 cut(s)	1144
PinAI	ACCGGT	1 cut(s)	478
PleI	GAGTC	3 cut(s)	494, 762, 1358
PluTI	GGCGCC	1 cut(s)	534
PpsI	GAGTC	3 cut(s)	494, 762, 1358
PpuMI	RGGWCCY	2 cut(s)	889, 1179
PsiI	TTATAA	1 cut(s)	1823
Psp124BI	GAGCTC	2 cut(s)	711, 981
Psp1406I	AACGTT	1 cut(s)	1817
Psp5II	RGGWCCY	2 cut(s)	889, 1179
Psp6I	CCWGG	2 cut(s)	1476, 1539
PspEI	GGTNACC	2 cut(s)	560, 827
PspFI	CCCAGC	1 cut(s)	1484
PspGI	CCWGG	2 cut(s)	1476, 1539
PspLI	CGTACG	1 cut(s)	1800
PspPI	GGNCC	6 cut(s)	589, 775, 889, 1179, 1537, 1582
PspPPI	RGGWCCY	2 cut(s)	889, 1179
PspXI	VCTCGAGB	1 cut(s)	1076
PsrI	GAACNNNNNNTAC	2 cut(s)	459, 491
PstI	CTGCAG	1 cut(s)	1161
PsuI	RGATCY	2 cut(s)	1221, 1546
PvuII	CAGCTG	2 cut(s)	928, 1783
RruI	TCGCGA	1 cut(s)	800
RsaI	GTAC	4 cut(s)	40, 64, 1631, 1802
RsaNI	GTAC	4 cut(s)	39, 63, 1630, 1801
RseI	CAYNNNNRTG	2 cut(s)	313, 1091
SacI	GAGCTC	2 cut(s)	711, 981
SalI	GTCGAC	2 cut(s)	1265, 1441
SapI	GCTCTTC	1 cut(s)	986
SaqAI	TTAA	1 cut(s)	48
Sau3AI	GATC	5 cut(s)	942, 1167, 1221, 1329, 1546
Sau96I	GGNCC	6 cut(s)	589, 775, 889, 1179, 1537, 1582
SchI	GAGTC	3 cut(s)	494, 762, 1358
ScrFI	CCNGG	3 cut(s)	1361, 1478, 1541
SduI	GDGCHC	3 cut(s)	459, 711, 981
SfaNI	GCATC	3 cut(s)	347, 735, 1083
SfcI	CTRYAG	2 cut(s)	33, 1157
SfoI	GGCGCC	1 cut(s)	532
Sfr274I	CTCGAG	2 cut(s)	1076, 1789
SfuI	TTCGAA	1 cut(s)	918
SinI	GGWCC	5 cut(s)	589, 775, 889, 1179, 1582
SlaI	CTCGAG	2 cut(s)	1076, 1789
SmiMI	CAYNNNNRTG	2 cut(s)	313, 1091
SmlI	CTYRAG	2 cut(s)	1076, 1789
SmoI	CTYRAG	2 cut(s)	1076, 1789
Sse9I	AATT	9 cut(s)	103, 119, 147, 201, 436, 1031, 1390, 1423, 1776
SseBI	AGGCCT	3 cut(s)	513, 1012, 1850
SspDI	GGCGCC	1 cut(s)	530
SspMI	CTAG	2 cut(s)	194, 1508
SstI	GAGCTC	2 cut(s)	711, 981
StuI	AGGCCT	3 cut(s)	513, 1012, 1850
StyD4I	CCNGG	3 cut(s)	1359, 1476, 1539
StyI	CCWWGG	5 cut(s)	342, 378, 508, 1239, 1752
TaaI	ACNGT	5 cut(s)	827, 901, 1619, 1634, 1805
TaiI	ACGT	6 cut(s)	522, 670, 870, 1002, 1125, 1820
TaqI	TCGA	9 cut(s)	465, 573, 918, 1065, 1077, 1227, 1266, 1442, 1790
TasI	AATT	9 cut(s)	103, 119, 147, 201, 436, 1031, 1390, 1423, 1776
TauI	GCSGC	4 cut(s)	29, 909, 1452, 1455
TfiI	GAWTC	3 cut(s)	1254, 1468, 1495
Tru1I	TTAA	1 cut(s)	48
Tru9I	TTAA	1 cut(s)	48
TscAI	CASTG	2 cut(s)	309, 1597
TseFI	GTSAC	5 cut(s)	481, 560, 668, 827, 1619
TseI	GCWGC	7 cut(s)	29, 335, 925, 1081, 1156, 1735, 1783
Tsp45I	GTSAC	5 cut(s)	481, 560, 668, 827, 1619
TspDTI	ATGAA	2 cut(s)	1392, 1799
TspGWI	ACGGA	4 cut(s)	627, 1337, 1539, 1788
TspRI	CASTG	2 cut(s)	309, 1597
Van91I	CCANNNNNTGG	1 cut(s)	1144
VpaK11BI	GGWCC	5 cut(s)	589, 775, 889, 1179, 1582
XapI	RAATTY	5 cut(s)	103, 201, 436, 1031, 1390
XhoI	CTCGAG	2 cut(s)	1076, 1789
XmiI	GTMKAC	3 cut(s)	816, 1266, 1442
XmnI	GAANNNNTTC	1 cut(s)	1025
XspI	CTAG	2 cut(s)	194, 1508
ZraI	GACGTC	1 cut(s)	1000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.