pycom10g17330

Protein Iojap, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013563
Physical Location & Seq
Forward (+)
20528039 .. 20530110
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGACTGATCCATCTCGTGAGTATATTCTCTCTCATCTTCAAATATCTTTGCAACCGTACCAAACCAAAACACCAAAAATGGCGGTTCTCATTACTCTGTCTCTTGCCGGAGCTGGCGTCGGATACCGGTTCTCCGACGACCTCCAGAAGCTAGGTCGTGTTCAACCTAAGCTCTATCAAAGTCCCGCCGCGCTTTTTCCTCATAAGTGCTTGCACTGCAAGTGCTTTTGGCAAGTGCCCAGAAGCAAGAACCTAACTTTGAAGTCAAGGGAATTGCCAAGATTACGTTTGGCTCGCGCCAAGGAAGCTGAAGATAGTTTCACTCCGAATGTAAGCGAAGATACGGACGATATGTTTGATGACTTGTTTGATAAATATGGAAAGGTGGTATATAGTAGCAATGACAGGAAGTCTCCTAGCACAGAGCTTGATGATGATGCTGAAAGCTTGTCATTTGCTGTTGCAATGGCCAATGTTGCAAATGACGTAAAAGCAGGAGATATAAAGGTCCTATTTGTGAAGCCTCTTGTATATTGGACTCGGTTTTTTATCATTGCCACGGCATTTTCTCGTCCGCAAAGTGATGCAATTGCGTCCAGGATAAGAGATATGGCTGAGGAAAAGTATGGAAGAGTTCCATCTGGGGACTCAAAACCCAACTCATGGACCCTGTTGGACTTTGGTGATGTTGTTATTCATATATTTCTTCCACCACAGCGAGCTTTCTACAACTTGGAAGAATTCTACGCCAACGCGACACCCGTTGAATTGCCTTTTGAGAATCAGTCACCTTTTCGAGGCTGA

Protein Analysis

268

Amino Acids

30.26

Weight (kDa)

6.45

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsfS PF02410 156 - 249 7.6e-23 Ribosomal silencing factor during starvation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013563)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12930
fragaria_vesca FvH4_3g15760 FvH4_3g15760
malus_domestica MD10G1202500.v1.1
prunus_persica Prupe.4G139700_v2.0.a1 Prupe.4G139700_v2.0.a1
pyrus_communis pycom05g20150 pycom10g17330
rosa_chinensis RchiOBHm_Chr5g0026561
rosa_laevigata RLG00000032935
rosa_multiflora Rmu_sc0004371.1_g000009
rosa_roxburghii Rroxscaffold_1G00052920
rosa_rugosa Rorug05G0093800
rosa_samantha Rh5AG186700 Rh5BG183700 Rh5CG203600 Rh5DG186000
rosa_wichuraiana Rw5G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 663
AccII CGCG 3 cut(s) 191, 297, 755
AciI CCGC 4 cut(s) 83, 186, 189, 575
AclWI GGATC 1 cut(s) 2
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 663, 797
AgeI ACCGGT 1 cut(s) 126
AgsI TTSAA 4 cut(s) 41, 164, 262, 767
AhdI GACNNNNNGTC 1 cut(s) 409
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 7 cut(s) 113, 151, 172, 308, 427, 447, 722
AluI AGCT 7 cut(s) 113, 151, 172, 308, 427, 447, 722
Alw26I GTCTC 2 cut(s) 105, 417
AlwI GGATC 1 cut(s) 2
AoxI GGCC 1 cut(s) 468
ApoI RAATTY 1 cut(s) 740
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AsiGI ACCGGT 1 cut(s) 126
AspLEI GCGC 2 cut(s) 193, 299
AspS9I GGNCC 2 cut(s) 508, 666
AsuHPI GGTGA 2 cut(s) 695, 780
AvaII GGWCC 2 cut(s) 508, 666
BaeGI GKGCMC 1 cut(s) 240
BalI TGGCCA 1 cut(s) 470
BauI CACGAG 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 615
BccI CCATC 2 cut(s) 19, 646
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 1 cut(s) 598
BciVI GTATCC 1 cut(s) 116
BcoDI GTCTC 2 cut(s) 105, 417
BfaI CTAG 2 cut(s) 152, 417
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 598
Bme18I GGWCC 2 cut(s) 508, 666
BmeRI GACNNNNNGTC 1 cut(s) 409
BmgT120I GGNCC 2 cut(s) 508, 666
BmiI GGNNCC 1 cut(s) 668
BmrFI CCNGG 1 cut(s) 598
BmsI GCATC 2 cut(s) 427, 574
BpmI CTGGAG 1 cut(s) 128
Bpu10I CCTNAGC 2 cut(s) 168, 615
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 300, 558
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 4 cut(s) 102, 116, 132, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 663, 797
Bse118I RCCGGY 1 cut(s) 126
Bse3DI GCAATG 3 cut(s) 406, 471, 552
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 2 cut(s) 300, 558
BseLI CCNNNNNNNGG 2 cut(s) 663, 797
BseMI GCAATG 3 cut(s) 406, 471, 552
BseMII CTCAG 1 cut(s) 606
BseSI GKGCMC 1 cut(s) 240
Bsh1236I CGCG 3 cut(s) 191, 297, 755
BshFI GGCC 1 cut(s) 470
BshTI ACCGGT 1 cut(s) 126
BsiSI CCGG 2 cut(s) 108, 127
BslFI GGGAC 2 cut(s) 168, 659
BslI CCNNNNNNNGG 2 cut(s) 663, 797
BsmAI GTCTC 2 cut(s) 105, 417
BsmFI GGGAC 2 cut(s) 168, 659
BsnI GGCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 1 cut(s) 7
BspACI CCGC 4 cut(s) 83, 186, 189, 575
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 3 cut(s) 191, 297, 755
BspLI GGNNCC 1 cut(s) 668
BspPI GGATC 1 cut(s) 2
BsrDI GCAATG 3 cut(s) 406, 471, 552
BsrFI RCCGGY 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 2 cut(s) 300, 558
BssMI GATC 1 cut(s) 7
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 1 cut(s) 57
Bst6I CTCTTC 1 cut(s) 625
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 115, 212, 295, 720
BstDEI CTNAG 2 cut(s) 168, 615
BstDSI CCRYGG 1 cut(s) 558
BstENI CCTNNNNNAGG 1 cut(s) 795
BstFNI CGCG 3 cut(s) 191, 297, 755
BstHHI GCGC 2 cut(s) 193, 299
BstKTI GATC 1 cut(s) 10
BstMAI GTCTC 2 cut(s) 105, 417
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 3 cut(s) 216, 305, 476
BstNI CCWGG 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 3 cut(s) 191, 297, 755
BsuI GTATCC 1 cut(s) 116
BsuRI GGCC 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 558
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 115, 212, 295, 720
CfoI GCGC 2 cut(s) 193, 299
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 2 cut(s) 508, 666
CseI GACGC 2 cut(s) 106, 582
Csp6I GTAC 1 cut(s) 58
CspAI ACCGGT 1 cut(s) 126
CviAII CATG 1 cut(s) 663
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 168, 615
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
DriI GACNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 625
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 508, 666
Eco57I CTGAAG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 508
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 596
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 666
FaqI GGGAC 2 cut(s) 168, 659
FatI CATG 1 cut(s) 662
FauI CCCGC 1 cut(s) 193
Fnu4HI GCNGC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 2 cut(s) 152, 417
GlaI GCGC 2 cut(s) 192, 298
GluI GCNGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 128
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 108, 127
HgaI GACGC 2 cut(s) 106, 582
HhaI GCGC 2 cut(s) 193, 299
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 666
Hin6I GCGC 2 cut(s) 191, 297
HinP1I GCGC 2 cut(s) 191, 297
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 3 cut(s) 538, 647, 781
HpaII CCGG 2 cut(s) 108, 127
HphI GGTGA 2 cut(s) 695, 780
Hpy188I TCNGA 3 cut(s) 122, 136, 327
Hpy188III TCNNGA 2 cut(s) 17, 145
Hpy99I CGWCG 2 cut(s) 122, 140
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4IV ACGT 2 cut(s) 286, 486
HpyCH4V TGCA 6 cut(s) 52, 214, 219, 464, 479, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 305, 476
HpyF3I CTNAG 2 cut(s) 168, 615
HpySE526I ACGT 2 cut(s) 286, 486
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 666
HspAI GCGC 2 cut(s) 191, 297
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 110
LweI GCATC 2 cut(s) 427, 574
MaeI CTAG 2 cut(s) 152, 417
MaeII ACGT 2 cut(s) 286, 486
MaeIII GTNAC 1 cut(s) 786
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 6 cut(s) 29, 323, 350, 642, 698, 749
MfeI CAATTG 1 cut(s) 588
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 4 cut(s) 272, 588, 740, 767
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 532, 641
MmeI TCCRAC 3 cut(s) 100, 159, 654
MnlI CCTC 5 cut(s) 152, 210, 534, 610, 791
Mox20I TGGCCA 1 cut(s) 470
MscI TGGCCA 1 cut(s) 470
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 2 cut(s) 108, 127
MspR9I CCNGG 1 cut(s) 598
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 598
MvnI CGCG 3 cut(s) 191, 297, 755
MwoI GCNNNNNNNGC 3 cut(s) 216, 305, 476
NdeII GATC 1 cut(s) 7
NlaIII CATG 1 cut(s) 666
NlaIV GGNNCC 1 cut(s) 668
NmuCI GTSAC 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PfeI GAWTC 1 cut(s) 781
PflMI CCANNNNNTGG 1 cut(s) 663
PfoI TCCNGGA 1 cut(s) 596
PinAI ACCGGT 1 cut(s) 126
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 2 cut(s) 532, 641
PpsI GAGTC 2 cut(s) 532, 641
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 596
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 1 cut(s) 668
PspPI GGNCC 2 cut(s) 508, 666
PspPPI RGGWCCY 1 cut(s) 508
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 2 cut(s) 508, 666
SchI GAGTC 2 cut(s) 532, 641
ScrFI CCNGG 1 cut(s) 598
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 2 cut(s) 427, 574
SinI GGWCC 2 cut(s) 508, 666
Sse9I AATT 4 cut(s) 272, 588, 740, 767
SsiI CCGC 4 cut(s) 83, 186, 189, 575
SspMI CTAG 2 cut(s) 152, 417
StyD4I CCNGG 1 cut(s) 596
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 57
TaiI ACGT 2 cut(s) 289, 489
TaqI TCGA 1 cut(s) 796
TasI AATT 4 cut(s) 272, 588, 740, 767
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 1 cut(s) 781
TscAI CASTG 1 cut(s) 221
TseFI GTSAC 1 cut(s) 786
Tsp45I GTSAC 1 cut(s) 786
TspDTI ATGAA 1 cut(s) 686
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 663
VpaK11BI GGWCC 2 cut(s) 508, 666
XagI CCTNNNNNAGG 1 cut(s) 795
XapI RAATTY 1 cut(s) 740
XspI CTAG 2 cut(s) 152, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.