pycom10g18240

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008663
Physical Location & Seq
Forward (+)
21545919 .. 21550262
4344 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
ATGGACTATGACCCGATCCATGTGATGCAAACCGGTGATGATAAATCCTTGAGTTTTGAGACAGTGCTCTTCTCTGAGCTGCTGAAAAATATGCAGCATCTTCTTCCGGCCCGCCTCATAAAGTCTCTGTCTGCTACTTCTTTACCATGCACCACCTCCACCCTTCACGAACTTGCTTCTACTCACCCCAAAGGTGTTGCGAGGGTTGTACTGAAAAAGGGGAAGACCCAATTATTCAAGGATGGCAGCCCAATGGTCTACAGTGGAGCAGTTGATAGAATAATTGGTAGACCACCTCCCAGGACTGGAGACATTGTGCTGGTAGCTGATGGGGCAGAAAAACCAATAGGATGGGGCATGTACAATTCAGTCTCCATGTTCAGTGTTCGGCTGATGCAACTCGAAGAGGAAGCAACAAGGGATTTGTCATGTGCATTGAACATGGAGAAACTGCTTGAGACAAGAATTAATGAAGCTATAGAACTACGTAAGAGTTTGGGACTGCCATCAGTTAGTACAAATGCATTCCGTCTTGTCAATAGTGAAGCAGACAGATTGTCAGGATTAATAGTTGATATATTTGGAGATGTAGCGGTAGTAGCATCGTCTGCTGCTTGGGTTGAGAAGTACAAAGCAGAAGTAGAGGCCTGTATTAGTAGAATTGACGAAGTTAATTATGTAAACTGGAGACCATCTTTGGAAGTTTTAAAAGAAGAAGGATTGGATGTGTCAAATTACAAGGAAAGGGATCCATCTACCTGTCCTCAAAGAACAAAGGTTATGGAAAACGGGATCTTTTATTCCATTTCACTTGAGGGACAGAAGACAGGGTTTTATGCGGATCAGCGTGAAAACCGCCAATTCATATCAACAATTTCAGAAGGCCAGAAAGTTCTTGATATGTTCTGCTACAGTGGTGGTTTTGCTCTAAATGCTGCACGAGGGGGTGCTGCAAATGTCATCGGTGTCGATTCATCATTGCCTGCGATTGAGCTAGCCAAAGAAAATGTTGTTCTTAACAACATGGATCCACAGAGAATAACATTTTTGAAAGAAGATGCAAGTGAGTTTATGAAGGGTGCTCTTTCTAGGAACGAATCATGGGATATTGTCATTTTCGATCCTCCTAAACTAGCACCCCGAAAGAAGGTTTTACAAAATGCATCAGGCATGTATAGAAATTTAAATTCATTGGCAATGCAACTGACAAAGAGAGGTGGTCTTCTCATGACTTGTTCATGTTCGGGGGCTATGACGCAAAGTGGGATGTTCTTGCGTATTCTTCAGGGTGCAGCATCAGCGGCAGGGAGAAAAATAACCGTTCTACGGGACGCTGGAGCGGCATGTGATCATCCCATTGACCCATCCTACCCAGAAGGCAAATACCTTTCTAACGTCCTTCTGAGGGTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

472

Amino Acids

51.71

Weight (kDa)

6.57

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 69 - 132 3.1e-10 PUA-like domain
Methyltrans_SAM PF10672 259 - 419 6.2e-17 S-adenosylmethionine-dependent methyltransferase
TRM5-TYW2_MTfase PF02475 260 - 363 3.1e-08 TRM5/TYW2 methyltransferase domain
PrmA PF06325 292 - 371 7.9e-08 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 293 - 415 1.6e-10 Methyltransferase domain
Cons_hypoth95 PF03602 293 - 376 1.7e-08 Conserved hypothetical protein 95
Methyltransf_25 PF13649 298 - 373 1.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1340
AccI GTMKAC 2 cut(s) 258, 289
AciI CCGC 6 cut(s) 112, 593, 839, 856, 1301, 1340
AclWI GGATC 8 cut(s) 10, 743, 756, 800, 849, 1022, 1035, 1115
AcsI RAATTY 2 cut(s) 1180, 1186
AcuI CTGAAG 1 cut(s) 1268
AfaI GTAC 4 cut(s) 210, 362, 517, 629
AfiI CCNNNNNNNGG 4 cut(s) 305, 1147, 1326, 1405
AgeI ACCGGT 1 cut(s) 32
AgsI TTSAA 3 cut(s) 238, 439, 1051
AhdI GACNNNNNGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 4 cut(s) 79, 326, 476, 994
AluI AGCT 4 cut(s) 79, 326, 476, 994
Alw21I GWGCWC 2 cut(s) 69, 1084
Alw26I GTCTC 6 cut(s) 53, 129, 303, 376, 452, 682
AlwI GGATC 8 cut(s) 10, 743, 756, 800, 849, 1022, 1035, 1115
AoxI GGCC 3 cut(s) 108, 645, 883
ApeKI GCWGC 7 cut(s) 79, 94, 246, 611, 935, 950, 1292
ApoI RAATTY 2 cut(s) 1180, 1186
AseI ATTAAT 2 cut(s) 468, 566
AsiGI ACCGGT 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 109, 1408
AsuHPI GGTGA 2 cut(s) 47, 176
AsuNHI GCTAGC 1 cut(s) 994
AvaII GGWCC 1 cut(s) 1408
BamHI GGATCC 2 cut(s) 748, 1027
BauI CACGAG 1 cut(s) 939
BbsI GAAGAC 3 cut(s) 230, 830, 1214
Bbv12I GWGCWC 2 cut(s) 69, 1084
BbvI GCAGC 7 cut(s) 66, 106, 258, 598, 922, 937, 1304
BccI CCATC 7 cut(s) 236, 323, 345, 514, 700, 760, 1372
BciT130I CCWGG 1 cut(s) 301
BclI TGATCA 1 cut(s) 1348
BcoDI GTCTC 6 cut(s) 53, 129, 303, 376, 452, 682
BfaI CTAG 3 cut(s) 995, 1089, 1133
BfmI CTRYAG 3 cut(s) 259, 477, 910
BisI GCNGC 9 cut(s) 80, 95, 247, 612, 936, 951, 1293, 1302, 1341
BlsI GCNGC 9 cut(s) 81, 96, 248, 613, 937, 952, 1294, 1303, 1342
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 1408
BmeRI GACNNNNNGTC 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 109, 1408
BmiI GGNNCC 3 cut(s) 750, 1029, 1409
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 7 cut(s) 15, 106, 384, 611, 1048, 1172, 1304
BmtI GCTAGC 1 cut(s) 998
BpiI GAAGAC 3 cut(s) 230, 830, 1214
BpmI CTGGAG 3 cut(s) 327, 706, 1356
BpuEI CTTGAG 3 cut(s) 70, 476, 833
BsaAI YACGTR 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 4 cut(s) 305, 1147, 1326, 1405
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 310, 689
Bse3DI GCAATG 2 cut(s) 977, 1203
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 6 cut(s) 247, 356, 730, 1272, 1351, 1364
BseLI CCNNNNNNNGG 4 cut(s) 305, 1147, 1326, 1405
BseMI GCAATG 2 cut(s) 977, 1203
BseMII CTCAG 2 cut(s) 66, 1394
BseNI ACTGG 2 cut(s) 310, 689
BseXI GCAGC 7 cut(s) 66, 106, 258, 598, 922, 937, 1304
BsgI GTGCAG 2 cut(s) 921, 1311
BshFI GGCC 3 cut(s) 110, 647, 885
BshTI ACCGGT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 69, 1084
BsiSI CCGG 2 cut(s) 33, 107
BslFI GGGAC 3 cut(s) 513, 831, 1343
BslI CCNNNNNNNGG 4 cut(s) 305, 1147, 1326, 1405
BsmAI GTCTC 6 cut(s) 53, 129, 303, 376, 452, 682
BsmFI GGGAC 3 cut(s) 513, 831, 1343
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 3 cut(s) 110, 647, 885
Bso31I GGTCTC 1 cut(s) 682
Bsp1286I GDGCHC 2 cut(s) 69, 1084
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
BspACI CCGC 6 cut(s) 112, 593, 839, 856, 1301, 1340
BspANI GGCC 3 cut(s) 110, 647, 885
BspCNI CTCAG 2 cut(s) 67, 1395
BspHI TCATGA 1 cut(s) 1227
BspLI GGNNCC 3 cut(s) 750, 1029, 1409
BspOI GCTAGC 1 cut(s) 998
BspPI GGATC 8 cut(s) 10, 743, 756, 800, 849, 1022, 1035, 1115
BspQI GCTCTTC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 682
BsrBI CCGCTC 1 cut(s) 1340
BsrDI GCAATG 2 cut(s) 977, 1203
BsrFI RCCGGY 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 2 cut(s) 310, 689
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
BssSI CACGAG 1 cut(s) 939
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 4 cut(s) 64, 263, 914, 1321
Bst6I CTCTTC 2 cut(s) 74, 399
BstAPI GCANNNNNTGC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 3 cut(s) 112, 984, 996
BstDEI CTNAG 2 cut(s) 75, 1403
BstF5I GGATG 6 cut(s) 247, 356, 730, 1272, 1351, 1364
BstKTI GATC 7 cut(s) 18, 751, 795, 844, 1030, 1123, 1351
BstMAI GTCTC 6 cut(s) 53, 129, 303, 376, 452, 682
BstMBI GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
BstMWI GCNNNNNNNGC 7 cut(s) 332, 599, 608, 932, 1298, 1301, 1340
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 3 cut(s) 361, 1174, 1347
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 3 cut(s) 259, 477, 910
BstSNI TACGTA 1 cut(s) 488
BstV1I GCAGC 7 cut(s) 66, 106, 258, 598, 922, 937, 1304
BstV2I GAAGAC 3 cut(s) 230, 830, 1214
BstX2I RGATCY 3 cut(s) 748, 792, 1027
BstXI CCANNNNNNTGG 1 cut(s) 351
BstYI RGATCY 3 cut(s) 748, 792, 1027
BsuRI GGCC 3 cut(s) 110, 647, 885
BtsCI GGATG 6 cut(s) 247, 356, 730, 1272, 1351, 1364
BtsIMutI CAGTG 4 cut(s) 69, 268, 388, 919
Cac8I GCNNGC 3 cut(s) 112, 984, 996
CciI TCATGA 1 cut(s) 1227
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 109, 1408
CseI GACGC 2 cut(s) 1264, 1340
Csp6I GTAC 4 cut(s) 209, 361, 516, 628
CspAI ACCGGT 1 cut(s) 32
CviQI GTAC 4 cut(s) 209, 361, 516, 628
DdeI CTNAG 2 cut(s) 75, 1403
DpnI GATC 7 cut(s) 17, 750, 794, 843, 1029, 1122, 1350
DpnII GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
DraI TTTAAA 2 cut(s) 708, 1185
DriI GACNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 74, 399
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 2 cut(s) 74, 399
Eco105I TACGTA 1 cut(s) 488
Eco147I AGGCCT 1 cut(s) 647
Eco31I GGTCTC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 1408
Eco57I CTGAAG 1 cut(s) 1268
EcoO109I RGGNCCY 1 cut(s) 1408
EcoRII CCWGG 1 cut(s) 299
EcoT22I ATGCAT 2 cut(s) 526, 1165
FaqI GGGAC 3 cut(s) 513, 831, 1343
FauI CCCGC 1 cut(s) 119
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 2 cut(s) 258, 289
Fnu4HI GCNGC 9 cut(s) 80, 95, 247, 612, 936, 951, 1293, 1302, 1341
FokI GGATG 6 cut(s) 254, 363, 737, 1279, 1338, 1351
Fsp4HI GCNGC 9 cut(s) 80, 95, 247, 612, 936, 951, 1293, 1302, 1341
FspBI CTAG 3 cut(s) 995, 1089, 1133
GluI GCNGC 9 cut(s) 80, 95, 247, 612, 936, 951, 1293, 1302, 1341
GsuI CTGGAG 3 cut(s) 327, 706, 1356
HaeIII GGCC 3 cut(s) 110, 647, 885
HapII CCGG 2 cut(s) 33, 107
HgaI GACGC 2 cut(s) 1264, 1340
HinfI GANTC 2 cut(s) 971, 1097
HpaII CCGG 2 cut(s) 33, 107
HphI GGTGA 2 cut(s) 47, 176
Hpy166II GTNNAC 3 cut(s) 259, 290, 682
Hpy188I TCNGA 3 cut(s) 76, 880, 1404
Hpy188III TCNNGA 4 cut(s) 167, 561, 896, 1228
Hpy8I GTNNAC 3 cut(s) 259, 290, 682
HpyAV CCTTC 7 cut(s) 173, 710, 875, 1069, 1141, 1370, 1409
HpyCH4III ACNGT 4 cut(s) 64, 263, 914, 1321
HpyCH4IV ACGT 2 cut(s) 487, 1395
HpyF10VI GCNNNNNNNGC 7 cut(s) 332, 599, 608, 932, 1298, 1301, 1340
HpyF3I CTNAG 2 cut(s) 75, 1403
HpySE526I ACGT 2 cut(s) 487, 1395
Ksp22I TGATCA 1 cut(s) 1348
Kzo9I GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 2 cut(s) 266, 1337
Lsp1109I GCAGC 7 cut(s) 66, 106, 258, 598, 922, 937, 1304
LweI GCATC 7 cut(s) 15, 106, 384, 611, 1048, 1172, 1304
MaeI CTAG 3 cut(s) 995, 1089, 1133
MaeII ACGT 2 cut(s) 487, 1395
MalI GATC 7 cut(s) 17, 750, 794, 843, 1029, 1122, 1350
MbiI CCGCTC 1 cut(s) 1340
MboI GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
MflI RGATCY 3 cut(s) 748, 792, 1027
MhlI GDGCHC 2 cut(s) 69, 1084
Mph1103I ATGCAT 2 cut(s) 526, 1165
MseI TTAA 6 cut(s) 468, 566, 672, 707, 1017, 1184
MspA1I CMGCKG 1 cut(s) 1301
MspI CCGG 2 cut(s) 33, 107
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 524
MvaI CCWGG 1 cut(s) 301
MwoI GCNNNNNNNGC 7 cut(s) 332, 599, 608, 932, 1298, 1301, 1340
NdeII GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
NheI GCTAGC 1 cut(s) 994
NlaIV GGNNCC 3 cut(s) 750, 1029, 1409
NsiI ATGCAT 2 cut(s) 526, 1165
NspI RCATGY 3 cut(s) 361, 1174, 1347
PagI TCATGA 1 cut(s) 1227
PceI AGGCCT 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 74
PctI GAATGC 1 cut(s) 524
PfeI GAWTC 2 cut(s) 971, 1097
PinAI ACCGGT 1 cut(s) 32
PkrI GCNGC 9 cut(s) 81, 96, 248, 613, 937, 952, 1294, 1303, 1342
Ppu21I YACGTR 1 cut(s) 488
PpuMI RGGWCCY 1 cut(s) 1408
PshBI ATTAAT 2 cut(s) 468, 566
Psp5II RGGWCCY 1 cut(s) 1408
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 3 cut(s) 750, 1029, 1409
PspPI GGNCC 2 cut(s) 109, 1408
PspPPI RGGWCCY 1 cut(s) 1408
PsuI RGATCY 3 cut(s) 748, 792, 1027
RsaI GTAC 4 cut(s) 210, 362, 517, 629
RsaNI GTAC 4 cut(s) 209, 361, 516, 628
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 6 cut(s) 468, 566, 672, 707, 1017, 1184
SatI GCNGC 9 cut(s) 80, 95, 247, 612, 936, 951, 1293, 1302, 1341
Sau3AI GATC 7 cut(s) 15, 748, 792, 841, 1027, 1120, 1348
Sau96I GGNCC 2 cut(s) 109, 1408
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 2 cut(s) 69, 1084
SfaNI GCATC 7 cut(s) 15, 106, 384, 611, 1048, 1172, 1304
SfcI CTRYAG 3 cut(s) 259, 477, 910
SinI GGWCC 1 cut(s) 1408
SmiI ATTTAAAT 1 cut(s) 1185
SmlI CTYRAG 3 cut(s) 49, 455, 812
SmoI CTYRAG 3 cut(s) 49, 455, 812
SnaBI TACGTA 1 cut(s) 488
SseBI AGGCCT 1 cut(s) 647
SsiI CCGC 6 cut(s) 112, 593, 839, 856, 1301, 1340
SspMI CTAG 3 cut(s) 995, 1089, 1133
StuI AGGCCT 1 cut(s) 647
StyD4I CCNGG 1 cut(s) 299
SwaI ATTTAAAT 1 cut(s) 1185
TaaI ACNGT 4 cut(s) 64, 263, 914, 1321
TaiI ACGT 2 cut(s) 490, 1398
TaqI TCGA 3 cut(s) 402, 969, 1119
TatI WGTACW 4 cut(s) 208, 360, 515, 627
TauI GCSGC 2 cut(s) 1304, 1343
TfiI GAWTC 2 cut(s) 971, 1097
Tru1I TTAA 6 cut(s) 468, 566, 672, 707, 1017, 1184
Tru9I TTAA 6 cut(s) 468, 566, 672, 707, 1017, 1184
TscAI CASTG 4 cut(s) 69, 268, 388, 919
TseI GCWGC 7 cut(s) 79, 94, 246, 611, 935, 950, 1292
TspDTI ATGAA 6 cut(s) 486, 853, 963, 1088, 1179, 1227
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 4 cut(s) 69, 268, 388, 919
VpaK11BI GGWCC 1 cut(s) 1408
VspI ATTAAT 2 cut(s) 468, 566
XapI RAATTY 2 cut(s) 1180, 1186
XceI RCATGY 3 cut(s) 361, 1174, 1347
XmiI GTMKAC 2 cut(s) 258, 289
XspI CTAG 3 cut(s) 995, 1089, 1133
Zsp2I ATGCAT 2 cut(s) 526, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.