Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Reverse (-)

22566593 .. 22574214

Gene Structure 7622 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2604 bp

ATGGCAAAATCGAGACAACATTTCTCTAGCCAAGATTCATCACTGTCACCAACTTCTGTGCGATCACGAGAATGGGAGGGTCCATCAAGATGGACTGAGTACTTGGGTCCAGAGGCAACTTCTCCAATGAATTTGAGGAGCTCCAGGAATTCTGGTCCTGATGGACAGGTCCATAGTTCAGGCGGCTCCCACAAAGGCTTGAACATGCAGTGGGTAGTTCAACTAACAGAAGTGGCAGAAGGCCTTATGGCTAAAATGTATAGGCTGAACCAAATTTTGGATTACCCGGATCCAGTTGGCCATGTATTTTCAGAAGCATTTTGGAAAGCTGGTGTGTTCCCAAACCATCCAAGAACTTGCTTGTTACTCTCAAAGAAGTTCCCTGAACATTATAGCAAGTTACAGCTTGAACGCATTGATAAGGTTTCTTGGGATGCCTTGCAAGAAAATGCAGAACTTCATTTACAAAGCTTAGAGCCATGGATCCAGCTGCTTCTTGATTTGATGGCATTCCGAGAACAAGCTCTACGACTTATATTGGATCTGAGCAGTACAGTTATTACCTTGTTGCCCCATCAGAACTCGCTTATACTACATGCATTTATGGATCTCTTTTGTTCCTTTGTACGCGTTAACCTTTTCTCGGAGAAGATACCAAGGAAAATGATGCTACAAATGTATAACCTTTTGCATTCCATGTCAAGAGACCGAGATTGTGATTTTTATCATCGGTTGGTTCAGTTTATAGACAGTTATGATCCACCGTTGAAAGGATTAAAAGAAGACCTAAATTTTGTGAGCCCACGTATTGGAGAGGTGCTAGAGGCTGTGGGACCTATTATTTTTTTGTCAACAGATACAAGAAAGCTCAGAAATGAAGGTTTTTTAAGTCCATATCATCCTCGATATCCAGACATACTTACAAATTCTGCTCATCCAATGAGAGCTCAGGATCTAGCAAATGTCACCTCTTATCGTGAATGGGTATTATTTGGGTATCTTGTTTGTCCTGATGAGCTTCTTCGCGTCACTAGCATTGATATTGCTTTGGTCGTATTGAAAGAAAATCTGGTTCTTACATTGTTCAGGGATGAGTATATACTTTTACATGAGGATTATCAGCTATATGTCCTGCCACGAATTTTAGAATCAAAGAAGATGGCAAAGTCTGGTCGTACGAAGCAAAAAGAAGCAGATCTGGAGTATAGTGTTGCTAAGCAAGTCGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTCTCATGTGATGCCATTCATCATGAGAGGAGAATCTTGCTAAAGCAAGAGATTGGAAGAATGGCGCTTTTCTTCATCGATCAGCCTAGTTTGTTGGCTCCAAACATACAGATGGTATTCTCTGCCTTGGCTTTTGCTCAATGTGAGGTCATCTGGTATTTCCAACATGTTGGGATTGCGTCATCAAAATCTAAAACCACTCGCATGGTACCTGTTGACATAGACCCAAGTGATCCAACTATCGGGTTCTTATTAGATGGAATGGACCATCTGTGCTGTTTAGTACGCAAGTACATTGCAGCGATTCGAGGTTATGCATTATCATACCTTTCTTCTTGTGCTGTTAGAATCCGGTCTTTGTTGAATACCCCTGGGATGGTGGCTCTTGACTTAGATTCAAGCTTGAAAGGTCTTTTTCAGCAGATTGTTCAGCACCTTGAAAACATACCAAAACCACAGGGTGAAAATGTATCTGCCATTACATGTGATCTAAGTGAATTTCGAAAGGATTGGTTATCAATATTGATGATAGTCACATCTTCCCGATCATCTATAAACATACGACATTTGGAGAAAGCTACAGTTTCTACTGGGAAAGAAGGCTTATTATCGGAAGGAAATGCTGCATACAATTGGTCCAGATGTGTTGACGAACTGGAAACTCAATTATCAGAGCATGCGAGTCTTAAGAAATTGTATTTCTACCATCAGCACCTTACAGCAGTCTTCAGGAATACTATGTTTGGCCCAGAAGGTCGCCCACAGCATTGCTGTGCATGGCTTGGCGTTGCAAGTAGTTTTCCAGAATGTGCTTCCCCAATTGTTCCAGAAGAGGTAACTAAAATTGGCAGGGATGCAGTCCTTTATGTTGAATCTCTGATTGAATCAATAATGGGAGGACTCGAGGGATTAATCAACATACTTGATTCTGAAGGAGGATTCGGTGCCCTTGAGATCCAGCTTCTCCCGGAGCAGGCAGCATATTATATGAATTATGCATCCAGAGTATCAAATCTTTCAGCTAAATCTCCAAAGGGCCCATCTGGTTTCCCTTTTCCTGGTCAGGAGAGTCATCCTGAAAATAATACTTCTATCAAAATGTTAGAAGCTGCAGTGCAGAGACTGACCAATTTATGCTCAGTTTTGAATGATATGGAGCCTATATGTGTACTAAACCATGTTTTTGTCTTAAGGGAGTACATGAGAGAATGCATCCTTGGGAACCTTAGGAGGAGATTGCTTTCAGCACTTAAAACTGATAATCGATCTCCAACGGCCTTCTGTTCTGGAGTCACTTATTCGCAGACACATAAGCATCATCCATCTGGCAGAGCAGCATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

868

Amino Acids

98.16

Weight (kDa)

6.45

Isoelectric Point (pI)

49.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	75 - 844	8e-199	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1468
AccB1I	GGYRCC	2 cut(s)	1468, 2204
AccB7I	CCANNNNNTGG	2 cut(s)	277, 809
AccII	CGCG	2 cut(s)	630, 1026
AciI	CCGC	1 cut(s)	183
AcoI	YGGCCR	1 cut(s)	298
AcsI	RAATTY	7 cut(s)	130, 148, 273, 790, 925, 1140, 1757
AcuI	CTGAAG	2 cut(s)	1972, 2211
AdeI	CACNNNGTG	1 cut(s)	1721
AfaI	GTAC	9 cut(s)	101, 553, 627, 1177, 1470, 1545, 1553, 2430, 2459
AfiI	CCNNNNNNNGG	8 cut(s)	277, 643, 770, 809, 1502, 1636, 2233, 2318
AflII	CTTAAG	2 cut(s)	1946, 2449
AflIII	ACRYGT	3 cut(s)	628, 1426, 1742
AjnI	CCWGG	3 cut(s)	143, 1630, 2317
AjuI	GAANNNNNNNTTGG	4 cut(s)	260, 292, 2460, 2492
Alw21I	GWGCWC	2 cut(s)	143, 949
Alw26I	GTCTC	3 cut(s)	7, 699, 2374
AlwNI	CAGNNNCTG	1 cut(s)	2383
Ama87I	CYCGRG	1 cut(s)	2162
AoxI	GGCC	5 cut(s)	241, 298, 2005, 2296, 2535
ApaI	GGGCCC	1 cut(s)	2300
ApeKI	GCWGC	6 cut(s)	490, 1559, 1883, 2237, 2369, 2594
ApoI	RAATTY	7 cut(s)	130, 148, 273, 790, 925, 1140, 1757
ArsI	GACNNNNNNTTYG	2 cut(s)	776, 808
AseI	ATTAAT	1 cut(s)	2171
Asp718I	GGTACC	1 cut(s)	1468
AspLEI	GCGC	1 cut(s)	1327
AsuC2I	CCSGG	2 cut(s)	287, 2228
AsuHPI	GGTGA	3 cut(s)	39, 958, 1733
AsuII	TTCGAA	1 cut(s)	1762
AvaI	CYCGRG	1 cut(s)	2162
AvaII	GGWCC	7 cut(s)	80, 107, 155, 169, 833, 1525, 1896
AxyI	CCTNAGG	1 cut(s)	2486
BaeGI	GKGCMC	2 cut(s)	2209, 2300
BalI	TGGCCA	1 cut(s)	300
BamHI	GGATCC	2 cut(s)	289, 483
BanI	GGYRCC	2 cut(s)	1468, 2204
BanII	GRGCYC	4 cut(s)	143, 803, 949, 2300
BauI	CACGAG	1 cut(s)	66
BbsI	GAAGAC	2 cut(s)	789, 1978
Bbv12I	GWGCWC	2 cut(s)	143, 949
BbvI	GCAGC	5 cut(s)	477, 1571, 1870, 2249, 2356
BceAI	ACGGC	1 cut(s)	2550
BciT130I	CCWGG	3 cut(s)	145, 1632, 2319
BcnI	CCSGG	2 cut(s)	287, 2228
BcoDI	GTCTC	3 cut(s)	7, 699, 2374
BfaI	CTAG	5 cut(s)	27, 821, 956, 1032, 1347
BfmI	CTRYAG	2 cut(s)	1839, 2370
BfoI	RGCGCY	1 cut(s)	1328
BfrI	CTTAAG	2 cut(s)	1946, 2449
BglII	AGATCT	1 cut(s)	1195
BisI	GCNGC	7 cut(s)	184, 491, 1560, 1884, 2238, 2370, 2595
BlpI	GCTNAGC	1 cut(s)	1215
BlsI	GCNGC	7 cut(s)	185, 492, 1561, 1885, 2239, 2371, 2596
BmcAI	AGTACT	1 cut(s)	101
Bme1390I	CCNGG	5 cut(s)	145, 287, 1632, 2228, 2319
Bme18I	GGWCC	7 cut(s)	80, 107, 155, 169, 833, 1525, 1896
BmeT110I	CYCGRG	1 cut(s)	2162
BmrFI	CCNGG	5 cut(s)	145, 287, 1632, 2228, 2319
BmrI	ACTGGG	1 cut(s)	1860
BmsI	GCATC	7 cut(s)	424, 657, 1261, 2104, 2267, 2481, 2584
BmuI	ACTGGG	1 cut(s)	1860
BpiI	GAAGAC	2 cut(s)	789, 1978
BpmI	CTGGAG	3 cut(s)	127, 1220, 2568
Bpu10I	CCTNAGC	1 cut(s)	948
Bpu1102I	GCTNAGC	1 cut(s)	1215
Bpu14I	TTCGAA	1 cut(s)	1762
BpuEI	CTTGAG	1 cut(s)	2231
BpuMI	CCSGG	2 cut(s)	287, 2228
Bsa29I	ATCGAT	2 cut(s)	1338, 2524
BsaAI	YACGTR	1 cut(s)	806
BsaBI	GATNNNNATC	1 cut(s)	723
BsaI	GGTCTC	1 cut(s)	699
BsaJI	CCNNGG	6 cut(s)	479, 656, 1386, 1630, 1631, 2476
BsaWI	WCCGGW	1 cut(s)	1611
Bsc4I	CCNNNNNNNGG	8 cut(s)	277, 643, 770, 809, 1502, 1636, 2233, 2318
Bse1I	ACTGG	3 cut(s)	293, 1855, 1920
Bse21I	CCTNAGG	1 cut(s)	2486
Bse3DI	GCAATG	2 cut(s)	1554, 2026
Bse8I	GATNNNNATC	1 cut(s)	723
BseBI	CCWGG	3 cut(s)	145, 1632, 2319
BseCI	ATCGAT	2 cut(s)	1338, 2524
BseDI	CCNNGG	6 cut(s)	479, 656, 1386, 1630, 1631, 2476
BseJI	GATNNNNATC	1 cut(s)	723
BseLI	CCNNNNNNNGG	8 cut(s)	277, 643, 770, 809, 1502, 1636, 2233, 2318
BseMI	GCAATG	2 cut(s)	1554, 2026
BseMII	CTCAG	5 cut(s)	87, 536, 883, 962, 2412
BseNI	ACTGG	3 cut(s)	293, 1855, 1920
BseRI	GAGGAG	3 cut(s)	151, 1303, 2506
BseSI	GKGCMC	2 cut(s)	2209, 2300
BseXI	GCAGC	5 cut(s)	477, 1571, 1870, 2249, 2356
BsgI	GTGCAG	1 cut(s)	2396
Bsh1236I	CGCG	2 cut(s)	630, 1026
BshFI	GGCC	5 cut(s)	243, 300, 2007, 2298, 2537
BshNI	GGYRCC	2 cut(s)	1468, 2204
BshVI	ATCGAT	2 cut(s)	1338, 2524
BsiHKAI	GWGCWC	2 cut(s)	143, 949
BsiHKCI	CYCGRG	1 cut(s)	2162
BsiSI	CCGG	3 cut(s)	287, 1612, 2228
BsiWI	CGTACG	1 cut(s)	1175
BslFI	GGGAC	1 cut(s)	846
BslI	CCNNNNNNNGG	8 cut(s)	277, 643, 770, 809, 1502, 1636, 2233, 2318
BsmAI	GTCTC	3 cut(s)	7, 699, 2374
BsmFI	GGGAC	1 cut(s)	846
BsmI	GAATGC	3 cut(s)	509, 691, 2474
BsnI	GGCC	5 cut(s)	243, 300, 2007, 2298, 2537
Bso31I	GGTCTC	1 cut(s)	699
BsoBI	CYCGRG	1 cut(s)	2162
Bsp119I	TTCGAA	1 cut(s)	1762
Bsp120I	GGGCCC	1 cut(s)	2296
Bsp1286I	GDGCHC	5 cut(s)	143, 803, 949, 2209, 2300
Bsp1720I	GCTNAGC	1 cut(s)	1215
Bsp19I	CCATGG	1 cut(s)	479
BspACI	CCGC	1 cut(s)	183
BspANI	GGCC	5 cut(s)	243, 300, 2007, 2298, 2537
BspCNI	CTCAG	5 cut(s)	88, 537, 882, 961, 2411
BspDI	ATCGAT	2 cut(s)	1338, 2524
BspFNI	CGCG	2 cut(s)	630, 1026
BspHI	TCATGA	1 cut(s)	1282
BspMAI	CTGCAG	1 cut(s)	2374
BspT104I	TTCGAA	1 cut(s)	1762
BspT107I	GGYRCC	2 cut(s)	1468, 2204
BspTI	CTTAAG	2 cut(s)	1946, 2449
BspTNI	GGTCTC	1 cut(s)	699
BsrDI	GCAATG	2 cut(s)	1554, 2026
BsrI	ACTGG	3 cut(s)	293, 1855, 1920
BssECI	CCNNGG	6 cut(s)	479, 656, 1386, 1630, 1631, 2476
BssSI	CACGAG	1 cut(s)	66
BssT1I	CCWWGG	4 cut(s)	479, 656, 1386, 2476
Bst2BI	CACGAG	1 cut(s)	66
Bst2UI	CCWGG	3 cut(s)	145, 1632, 2319
Bst4CI	ACNGT	5 cut(s)	45, 556, 752, 765, 1843
Bst6I	CTCTTC	1 cut(s)	2085
BstAFI	CTTAAG	2 cut(s)	1946, 2449
BstBAI	YACGTR	1 cut(s)	806
BstBI	TTCGAA	1 cut(s)	1762
BstC8I	GCNNGC	3 cut(s)	1254, 1938, 2235
BstDSI	CCRYGG	1 cut(s)	479
BstFNI	CGCG	2 cut(s)	630, 1026
BstH2I	RGCGCY	1 cut(s)	1328
BstHHI	GCGC	1 cut(s)	1327
BstMAI	GTCTC	3 cut(s)	7, 699, 2374
BstMWI	GCNNNNNNNGC	1 cut(s)	1032
BstNI	CCWGG	3 cut(s)	145, 1632, 2319
BstNSI	RCATGY	5 cut(s)	208, 599, 1430, 1746, 1940
BstSCI	CCNGG	5 cut(s)	143, 285, 1630, 2226, 2317
BstSFI	CTRYAG	2 cut(s)	1839, 2370
BstSLI	GKGCMC	2 cut(s)	2209, 2300
BstUI	CGCG	2 cut(s)	630, 1026
BstV1I	GCAGC	5 cut(s)	477, 1571, 1870, 2249, 2356
BstV2I	GAAGAC	2 cut(s)	789, 1978
BstX2I	RGATCY	7 cut(s)	289, 483, 541, 607, 952, 1195, 2214
BstXI	CCANNNNNNTGG	3 cut(s)	90, 1430, 1465
BstYI	RGATCY	7 cut(s)	289, 483, 541, 607, 952, 1195, 2214
Bsu15I	ATCGAT	2 cut(s)	1338, 2524
Bsu36I	CCTNAGG	1 cut(s)	2486
BsuRI	GGCC	5 cut(s)	243, 300, 2007, 2298, 2537
BsuTUI	ATCGAT	2 cut(s)	1338, 2524
BtgI	CCRYGG	1 cut(s)	479
BtsI	GCAGTG	2 cut(s)	215, 2379
BtsIMutI	CAGTG	3 cut(s)	41, 215, 2379
Cac8I	GCNNGC	3 cut(s)	1254, 1938, 2235
CaiI	CAGNNNCTG	1 cut(s)	2383
CciI	TCATGA	1 cut(s)	1282
CfoI	GCGC	1 cut(s)	1327
ClaI	ATCGAT	2 cut(s)	1338, 2524
CseI	GACGC	2 cut(s)	1015, 1428
Csp6I	GTAC	9 cut(s)	100, 552, 626, 1176, 1469, 1544, 1552, 2429, 2458
CviQI	GTAC	9 cut(s)	100, 552, 626, 1176, 1469, 1544, 1552, 2429, 2458
DraIII	CACNNNGTG	1 cut(s)	1721
EaeI	YGGCCR	1 cut(s)	298
Eam1104I	CTCTTC	1 cut(s)	2085
EarI	CTCTTC	1 cut(s)	2085
Ecl136II	GAGCTC	2 cut(s)	141, 947
Eco130I	CCWWGG	4 cut(s)	479, 656, 1386, 2476
Eco147I	AGGCCT	1 cut(s)	243
Eco24I	GRGCYC	4 cut(s)	143, 803, 949, 2300
Eco31I	GGTCTC	1 cut(s)	699
Eco32I	GATATC	1 cut(s)	908
Eco47I	GGWCC	7 cut(s)	80, 107, 155, 169, 833, 1525, 1896
Eco53kI	GAGCTC	2 cut(s)	141, 947
Eco57I	CTGAAG	2 cut(s)	1972, 2211
Eco81I	CCTNAGG	1 cut(s)	2486
Eco88I	CYCGRG	1 cut(s)	2162
EcoICRI	GAGCTC	2 cut(s)	141, 947
EcoO109I	RGGNCCY	2 cut(s)	833, 2296
EcoRI	GAATTC	1 cut(s)	148
EcoRII	CCWGG	3 cut(s)	143, 1630, 2317
EcoRV	GATATC	1 cut(s)	908
EcoT14I	CCWWGG	4 cut(s)	479, 656, 1386, 2476
EcoT22I	ATGCAT	4 cut(s)	601, 1579, 2260, 2474
EcoT38I	GRGCYC	4 cut(s)	143, 803, 949, 2300
ErhI	CCWWGG	4 cut(s)	479, 656, 1386, 2476
FaqI	GGGAC	1 cut(s)	846
Fnu4HI	GCNGC	7 cut(s)	184, 491, 1560, 1884, 2238, 2370, 2595
FriOI	GRGCYC	4 cut(s)	143, 803, 949, 2300
Fsp4HI	GCNGC	7 cut(s)	184, 491, 1560, 1884, 2238, 2370, 2595
FspBI	CTAG	5 cut(s)	27, 821, 956, 1032, 1347
GlaI	GCGC	1 cut(s)	1326
GluI	GCNGC	7 cut(s)	184, 491, 1560, 1884, 2238, 2370, 2595
GsuI	CTGGAG	3 cut(s)	127, 1220, 2568
HaeII	RGCGCY	1 cut(s)	1328
HaeIII	GGCC	5 cut(s)	243, 300, 2007, 2298, 2537
HapII	CCGG	3 cut(s)	287, 1612, 2228
HgaI	GACGC	2 cut(s)	1015, 1428
HhaI	GCGC	1 cut(s)	1327
Hin6I	GCGC	1 cut(s)	1325
HinP1I	GCGC	1 cut(s)	1325
HincII	GTYRAC	4 cut(s)	634, 852, 1477, 1909
HindII	GTYRAC	4 cut(s)	634, 852, 1477, 1909
HindIII	AAGCTT	2 cut(s)	469, 1660
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	3 cut(s)	287, 1612, 2228
HphI	GGTGA	3 cut(s)	39, 958, 1733
Hpy166II	GTNNAC	5 cut(s)	634, 852, 1477, 1909, 2429
Hpy8I	GTNNAC	5 cut(s)	634, 852, 1477, 1909, 2429
HpyAV	CCTTC	7 cut(s)	233, 872, 1853, 1868, 2006, 2186, 2548
HpyCH4III	ACNGT	5 cut(s)	45, 556, 752, 765, 1843
HpyCH4IV	ACGT	1 cut(s)	805
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1032
HpySE526I	ACGT	1 cut(s)	805
HspAI	GCGC	1 cut(s)	1325
KpnI	GGTACC	1 cut(s)	1472
KspAI	GTTAAC	1 cut(s)	634
LmnI	GCTCC	6 cut(s)	138, 146, 191, 1363, 2230, 2416
Lsp1109I	GCAGC	5 cut(s)	477, 1571, 1870, 2249, 2356
LweI	GCATC	7 cut(s)	424, 657, 1261, 2104, 2267, 2481, 2584
MaeI	CTAG	5 cut(s)	27, 821, 956, 1032, 1347
MaeII	ACGT	1 cut(s)	805
MaeIII	GTNAC	8 cut(s)	45, 363, 399, 964, 1027, 1792, 2095, 2551
MfeI	CAATTG	2 cut(s)	1891, 2079
MflI	RGATCY	7 cut(s)	289, 483, 541, 607, 952, 1195, 2214
MhlI	GDGCHC	5 cut(s)	143, 803, 949, 2209, 2300
MlsI	TGGCCA	1 cut(s)	300
MluI	ACGCGT	1 cut(s)	628
MluNI	TGGCCA	1 cut(s)	300
MlyI	GAGTC	4 cut(s)	1951, 2154, 2338, 2559
MmeI	TCCRAC	3 cut(s)	1447, 1520, 2555
Mox20I	TGGCCA	1 cut(s)	300
Mph1103I	ATGCAT	4 cut(s)	601, 1579, 2260, 2474
MscI	TGGCCA	1 cut(s)	300
MseI	TTAA	7 cut(s)	633, 776, 887, 1947, 2171, 2450, 2511
MslI	CAYNNNNRTG	5 cut(s)	70, 88, 1370, 1463, 2031
Msp20I	TGGCCA	1 cut(s)	300
MspA1I	CMGCKG	1 cut(s)	490
MspCI	CTTAAG	2 cut(s)	1946, 2449
MspI	CCGG	3 cut(s)	287, 1612, 2228
MspR9I	CCNGG	5 cut(s)	145, 287, 1632, 2228, 2319
MunI	CAATTG	2 cut(s)	1891, 2079
Mva1269I	GAATGC	3 cut(s)	509, 691, 2474
MvaI	CCWGG	3 cut(s)	145, 1632, 2319
MvnI	CGCG	2 cut(s)	630, 1026
MwoI	GCNNNNNNNGC	1 cut(s)	1032
NciI	CCSGG	2 cut(s)	287, 2228
NcoI	CCATGG	1 cut(s)	479
NmuCI	GTSAC	5 cut(s)	45, 964, 1027, 1792, 2551
NsiI	ATGCAT	4 cut(s)	601, 1579, 2260, 2474
NspI	RCATGY	5 cut(s)	208, 599, 1430, 1746, 1940
NspV	TTCGAA	1 cut(s)	1762
PaeI	GCATGC	1 cut(s)	1940
PaeR7I	CTCGAG	1 cut(s)	2162
PagI	TCATGA	1 cut(s)	1282
PasI	CCCWGGG	1 cut(s)	1631
PceI	AGGCCT	1 cut(s)	243
PciI	ACATGT	2 cut(s)	1426, 1742
PctI	GAATGC	3 cut(s)	509, 691, 2474
Pfl23II	CGTACG	1 cut(s)	1175
PflMI	CCANNNNNTGG	2 cut(s)	277, 809
PfoI	TCCNGGA	2 cut(s)	143, 2226
PkrI	GCNGC	7 cut(s)	185, 492, 1561, 1885, 2239, 2371, 2596
PleI	GAGTC	4 cut(s)	1950, 2154, 2337, 2558
PpsI	GAGTC	4 cut(s)	1950, 2154, 2337, 2558
Ppu21I	YACGTR	1 cut(s)	806
PpuMI	RGGWCCY	1 cut(s)	833
PscI	ACATGT	2 cut(s)	1426, 1742
PshBI	ATTAAT	1 cut(s)	2171
Psp124BI	GAGCTC	2 cut(s)	143, 949
Psp5II	RGGWCCY	1 cut(s)	833
Psp6I	CCWGG	3 cut(s)	143, 1630, 2317
PspGI	CCWGG	3 cut(s)	143, 1630, 2317
PspLI	CGTACG	1 cut(s)	1175
PspOMI	GGGCCC	1 cut(s)	2296
PspPPI	RGGWCCY	1 cut(s)	833
PspXI	VCTCGAGB	1 cut(s)	2162
PsrI	GAACNNNNNNTAC	4 cut(s)	447, 479, 510, 542
PstI	CTGCAG	1 cut(s)	2374
PstNI	CAGNNNCTG	1 cut(s)	2383
PsuI	RGATCY	7 cut(s)	289, 483, 541, 607, 952, 1195, 2214
PvuII	CAGCTG	1 cut(s)	490
RsaI	GTAC	9 cut(s)	101, 553, 627, 1177, 1470, 1545, 1553, 2430, 2459
RsaNI	GTAC	9 cut(s)	100, 552, 626, 1176, 1469, 1544, 1552, 2429, 2458
RseI	CAYNNNNRTG	5 cut(s)	70, 88, 1370, 1463, 2031
SacI	GAGCTC	2 cut(s)	143, 949
SaqAI	TTAA	7 cut(s)	633, 776, 887, 1947, 2171, 2450, 2511
SatI	GCNGC	7 cut(s)	184, 491, 1560, 1884, 2238, 2370, 2595
ScaI	AGTACT	1 cut(s)	101
SchI	GAGTC	4 cut(s)	1951, 2154, 2338, 2559
ScrFI	CCNGG	5 cut(s)	145, 287, 1632, 2228, 2319
SduI	GDGCHC	5 cut(s)	143, 803, 949, 2209, 2300
SfaNI	GCATC	7 cut(s)	424, 657, 1261, 2104, 2267, 2481, 2584
SfcI	CTRYAG	2 cut(s)	1839, 2370
Sfr274I	CTCGAG	1 cut(s)	2162
SfuI	TTCGAA	1 cut(s)	1762
SinI	GGWCC	7 cut(s)	80, 107, 155, 169, 833, 1525, 1896
SlaI	CTCGAG	1 cut(s)	2162
SmiMI	CAYNNNNRTG	5 cut(s)	70, 88, 1370, 1463, 2031
SmlI	CTYRAG	4 cut(s)	1946, 2162, 2210, 2449
SmoI	CTYRAG	4 cut(s)	1946, 2162, 2210, 2449
SphI	GCATGC	1 cut(s)	1940
SseBI	AGGCCT	1 cut(s)	243
SsiI	CCGC	1 cut(s)	183
SspI	AATATT	1 cut(s)	1782
SspMI	CTAG	5 cut(s)	27, 821, 956, 1032, 1347
SstI	GAGCTC	2 cut(s)	143, 949
StuI	AGGCCT	1 cut(s)	243
StyD4I	CCNGG	5 cut(s)	143, 285, 1630, 2226, 2317
StyI	CCWWGG	4 cut(s)	479, 656, 1386, 2476
TaaI	ACNGT	5 cut(s)	45, 556, 752, 765, 1843
TaiI	ACGT	1 cut(s)	808
TaqI	TCGA	8 cut(s)	11, 904, 1224, 1338, 1567, 1762, 2163, 2524
TaqII	GACCGA	1 cut(s)	723
TatI	WGTACW	5 cut(s)	99, 551, 1551, 2428, 2457
TauI	GCSGC	1 cut(s)	186
Tru1I	TTAA	7 cut(s)	633, 776, 887, 1947, 2171, 2450, 2511
Tru9I	TTAA	7 cut(s)	633, 776, 887, 1947, 2171, 2450, 2511
TscAI	CASTG	3 cut(s)	48, 215, 2379
TseFI	GTSAC	5 cut(s)	45, 964, 1027, 1792, 2551
TseI	GCWGC	6 cut(s)	490, 1559, 1883, 2237, 2369, 2594
Tsp45I	GTSAC	5 cut(s)	45, 964, 1027, 1792, 2551
TspDTI	ATGAA	7 cut(s)	27, 143, 449, 891, 1268, 1324, 2264
TspRI	CASTG	3 cut(s)	48, 215, 2379
Van91I	CCANNNNNTGG	2 cut(s)	277, 809
Vha464I	CTTAAG	2 cut(s)	1946, 2449
VpaK11BI	GGWCC	7 cut(s)	80, 107, 155, 169, 833, 1525, 1896
VspI	ATTAAT	1 cut(s)	2171
XapI	RAATTY	7 cut(s)	130, 148, 273, 790, 925, 1140, 1757
XceI	RCATGY	5 cut(s)	208, 599, 1430, 1746, 1940
XhoI	CTCGAG	1 cut(s)	2162
XspI	CTAG	5 cut(s)	27, 821, 956, 1032, 1347
ZrmI	AGTACT	1 cut(s)	101
Zsp2I	ATGCAT	4 cut(s)	601, 1579, 2260, 2474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.