Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0011539

Physical Location & Seq

Genomic Coordinates Forward (+)

25990322 .. 25994817

Gene Structure 4496 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGGCTGGAGACAACGAAGCTGCAAGCACACTTGAAGCTACGCCAACATGGGCTGTAGCCACCGTTGTCTTTGTGTTGATAGTGGTCTCCACAATTATAGAGTATTTGCTTCATCTTTCACACAAGTATTTTCATAGGTTGAAAAGGAAGGCTCTCATTCAGGCTCTTGATAAGATCAAATCAGAATTGATGCTGCTAGGGTTCATATCGTTGCTACTAACCGTTGGTCAAAAGCCAATTGCAAATATCTGCATCTCTAAAAGCTTGGGTGATTCTTTTCTTCCTTGCAAGAGCTCCACCCACAGTGATGTCGAAGAGGAAACCAAATGCTCTAACCAAGGAAAAGTTTCTTTGATGTCCAGGGAGGGCGCGAATGAACTCCAATACTTAATTTTTGTGCTCGCTGCGTTCCACGTTTCGTCTTGTGTCCTCACCTTCGCTCTTGGTATGGCCAAGATGAAAAGGTGGGAGCTTTGGGAGGCAGAAACCAGAACATTGGAGTATCAATTTACTCACGATCCACGAAGGTTTCAGCTCGCACATCAAACATCATTTGGGAAGCGGCATTTGAGATGTTGGAGTGATCACGGATTCCTCCGTTGGCCGGTCAGCTTTCTGAGACAATTCTTTAATTCTGTATCAAGAGTGGATTACTTCACTCTCAGACATGGATTCATAACGGCACATTTTGCAGAAGGAAGCCGTTTTGACTTCCAGAAATATATAAGAAGAAATTTGGAGAAGGACTTTGGTGTGGTTATGGGATCAAGCTGGTGGATTTGGCTGTTCTCAGTATCTGTTGTCATATTCTTCACTGCAGATGGTTTTTACAATTATCTGTGGGTTCCCTTTATTCCGTTAGTGATGCTCTTGCTGGTGGGAACAAAGCTTCAGGAGATCATAACTAAAATGTGCCTAGACAGCCATGATAAATCTCATGTTGTTAGAGGAGCTTTGCTTGTGAAGCCCAGTGACCACTTTTTCTGGTTTAACTGGCCCCAGTTGCTTCTCCATCTTTTGCACTTCATTCTGTTTCAGAATTCCTTTCAGTTGGCATTCTTCACATGGACTTGGTACAAATTTGGGCTCACATCATGCTTCCACCATGAAACTGAGGATATTGTCATAAGGCTGGCCATGGGTGTAATGGTGCAAATCCTATGCGGCTATGTCACTCTGCCTCTCTATGCTTTGGTCACGCAGATGGGTACATCAATGAGAAAAGGCGTGTTTACTGCAAATGTGATGATAGGGCTGAATAAATGGAGAGCCAGGGCCAGGAAAAACCTAGCTCTTCGGAACCCGGCACGATTGTCGTTGGATGCTTCGCTTGAAACTTCACTATCAGTAAACACTTCACCTTCTTTCAGTGCTCATGAGGCTTCATTCTCCACCGTAGAATTTGGTCGTCCGTCCGACGATGCAGAATATGTAGCACTTGAAATACTGGAGGCCCAAAAAGCACAAGAAAAACAGGCAGCTGAGGGACAGCAAAGTAATGGTTCATTAGATCGATGGATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0016020 GO:0043207 GO:0044464 GO:0045087 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mlo

Protein Analysis

508

Amino Acids

58.11

Weight (kDa)

8.88

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mlo	PF03094	10 - 106	9.8e-37	Mlo family
Mlo	PF03094	106 - 439	7.5e-144	Mlo family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011539)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g08460 FvH4_3g08460 FvH4_3g08460
malus_domestica	MD10G1288000.v1.1
prunus_persica	Prupe.4G053700_v2.0.a1 Prupe.4G053700_v2.0.a1
pyrus_communis	pycom10g24100
rosa_chinensis	RchiOBHm_Chr5g0009371
rosa_laevigata	RLG00000031656
rosa_multiflora	Rmu_sc0002711.1_g000020
rosa_roxburghii	Rroxscaffold_1G00066720 Rroxscaffold_1G00067420
rosa_rugosa	Rorug04G0442100 Rorug04G0442200 Rorug04G0442300
rosa_samantha	Rh5AG073000 Rh5BG069400 Rh5CG081800 Rh5DG069600
rosa_wichuraiana	Rw5G006820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	371
AciI	CCGC	2 cut(s)	562, 1164
AclWI	GGATC	2 cut(s)	512, 772
AcoI	YGGCCR	3 cut(s)	450, 602, 1134
AcsI	RAATTY	4 cut(s)	733, 1039, 1079, 1400
AcuI	CTGAAG	1 cut(s)	875
AfaI	GTAC	2 cut(s)	1076, 1210
AfiI	CCNNNNNNNGG	2 cut(s)	604, 1278
AgsI	TTSAA	4 cut(s)	35, 142, 1334, 1442
AjnI	CCWGG	3 cut(s)	359, 1271, 1277
Alw21I	GWGCWC	3 cut(s)	296, 402, 1375
Alw26I	GTCTC	3 cut(s)	3, 91, 613
AlwI	GGATC	2 cut(s)	512, 772
AlwNI	CAGNNNCTG	1 cut(s)	797
AoxI	GGCC	6 cut(s)	450, 602, 995, 1134, 1275, 1452
ApeKI	GCWGC	4 cut(s)	20, 193, 404, 1478
ApoI	RAATTY	4 cut(s)	733, 1039, 1079, 1400
Asp700I	GAANNNNTTC	1 cut(s)	346
AspLEI	GCGC	1 cut(s)	371
AspS9I	GGNCC	3 cut(s)	996, 1275, 1453
AsuC2I	CCSGG	1 cut(s)	1304
AsuHPI	GGTGA	3 cut(s)	281, 424, 1350
BalI	TGGCCA	2 cut(s)	452, 1136
BanII	GRGCYC	2 cut(s)	296, 1089
Bbv12I	GWGCWC	3 cut(s)	296, 402, 1375
BbvCI	CCTCAGC	1 cut(s)	1482
BbvI	GCAGC	4 cut(s)	7, 180, 391, 1490
BccI	CCATC	4 cut(s)	815, 1020, 1198, 1509
BceAI	ACGGC	2 cut(s)	687, 696
BcgI	CGANNNNNNTGC	2 cut(s)	1296, 1330
BciT130I	CCWGG	3 cut(s)	361, 1273, 1279
BclI	TGATCA	1 cut(s)	583
BcnI	CCSGG	1 cut(s)	1304
BcoDI	GTCTC	3 cut(s)	3, 91, 613
BfaI	CTAG	3 cut(s)	197, 917, 1289
BfmI	CTRYAG	2 cut(s)	54, 816
BisI	GCNGC	6 cut(s)	21, 194, 405, 563, 1165, 1479
BlsI	GCNGC	6 cut(s)	22, 195, 406, 564, 1166, 1480
Bme1390I	CCNGG	4 cut(s)	361, 1273, 1279, 1304
BmgT120I	GGNCC	3 cut(s)	996, 1275, 1453
BmiI	GGNNCC	3 cut(s)	846, 998, 1301
BmrFI	CCNGG	4 cut(s)	361, 1273, 1279, 1304
BmrI	ACTGGG	2 cut(s)	963, 994
BmsI	GCATC	5 cut(s)	180, 261, 855, 1312, 1411
BmuI	ACTGGG	2 cut(s)	963, 994
BpmI	CTGGAG	2 cut(s)	27, 1469
Bpu10I	CCTNAGC	1 cut(s)	1482
BpuMI	CCSGG	1 cut(s)	1304
Bsa29I	ATCGAT	1 cut(s)	1513
BsaI	GGTCTC	1 cut(s)	91
BsaJI	CCNNGG	4 cut(s)	337, 360, 1137, 1272
Bsc4I	CCNNNNNNNGG	2 cut(s)	604, 1278
Bse118I	RCCGGY	1 cut(s)	604
Bse1I	ACTGG	4 cut(s)	969, 998, 1000, 1452
BseBI	CCWGG	3 cut(s)	361, 1273, 1279
BseCI	ATCGAT	1 cut(s)	1513
BseDI	CCNNGG	4 cut(s)	337, 360, 1137, 1272
BseGI	GGATG	1 cut(s)	1327
BseLI	CCNNNNNNNGG	2 cut(s)	604, 1278
BseMII	CTCAG	5 cut(s)	608, 676, 804, 1104, 1473
BseNI	ACTGG	4 cut(s)	969, 998, 1000, 1452
BseRI	GAGGAG	1 cut(s)	963
BseXI	GCAGC	4 cut(s)	7, 180, 391, 1490
Bsh1236I	CGCG	1 cut(s)	371
BshFI	GGCC	6 cut(s)	452, 604, 997, 1136, 1277, 1454
BshVI	ATCGAT	1 cut(s)	1513
BsiHKAI	GWGCWC	3 cut(s)	296, 402, 1375
BsiSI	CCGG	2 cut(s)	605, 1304
BslFI	GGGAC	1 cut(s)	1500
BslI	CCNNNNNNNGG	2 cut(s)	604, 1278
BsmAI	GTCTC	3 cut(s)	3, 91, 613
BsmFI	GGGAC	1 cut(s)	1500
BsmI	GAATGC	1 cut(s)	1055
BsnI	GGCC	6 cut(s)	452, 604, 997, 1136, 1277, 1454
Bso31I	GGTCTC	1 cut(s)	91
Bsp1286I	GDGCHC	4 cut(s)	296, 402, 1089, 1375
Bsp143I	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
Bsp19I	CCATGG	1 cut(s)	1137
BspACI	CCGC	2 cut(s)	562, 1164
BspANI	GGCC	6 cut(s)	452, 604, 997, 1136, 1277, 1454
BspCNI	CTCAG	5 cut(s)	609, 675, 803, 1105, 1474
BspDI	ATCGAT	1 cut(s)	1513
BspFNI	CGCG	1 cut(s)	371
BspHI	TCATGA	1 cut(s)	1375
BspLI	GGNNCC	3 cut(s)	846, 998, 1301
BspMAI	CTGCAG	1 cut(s)	820
BspPI	GGATC	2 cut(s)	512, 772
BspQI	GCTCTTC	1 cut(s)	1299
BspTNI	GGTCTC	1 cut(s)	91
BsrFI	RCCGGY	1 cut(s)	604
BsrI	ACTGG	4 cut(s)	969, 998, 1000, 1452
BssAI	RCCGGY	1 cut(s)	604
BssECI	CCNNGG	4 cut(s)	337, 360, 1137, 1272
BssMI	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
BssT1I	CCWWGG	2 cut(s)	337, 1137
Bst2UI	CCWGG	3 cut(s)	361, 1273, 1279
Bst4CI	ACNGT	4 cut(s)	64, 223, 305, 1396
Bst6I	CTCTTC	2 cut(s)	309, 1299
BstAPI	GCANNNNNTGC	1 cut(s)	689
BstC8I	GCNNGC	4 cut(s)	25, 402, 537, 1134
BstDEI	CTNAG	5 cut(s)	617, 662, 790, 1113, 1482
BstDSI	CCRYGG	1 cut(s)	1137
BstF5I	GGATG	1 cut(s)	1327
BstFNI	CGCG	1 cut(s)	371
BstHHI	GCGC	1 cut(s)	371
BstKTI	GATC	7 cut(s)	177, 520, 586, 767, 900, 1513, 1521
BstMAI	GTCTC	3 cut(s)	3, 91, 613
BstMBI	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
BstMWI	GCNNNNNNNGC	6 cut(s)	689, 921, 964, 1003, 1379, 1460
BstNI	CCWGG	3 cut(s)	361, 1273, 1279
BstSCI	CCNGG	4 cut(s)	359, 1271, 1277, 1302
BstSFI	CTRYAG	2 cut(s)	54, 816
BstUI	CGCG	1 cut(s)	371
BstV1I	GCAGC	4 cut(s)	7, 180, 391, 1490
BstX2I	RGATCY	1 cut(s)	1518
BstXI	CCANNNNNNTGG	1 cut(s)	496
BstYI	RGATCY	1 cut(s)	1518
Bsu15I	ATCGAT	1 cut(s)	1513
BsuRI	GGCC	6 cut(s)	452, 604, 997, 1136, 1277, 1454
BsuTUI	ATCGAT	1 cut(s)	1513
BtgI	CCRYGG	1 cut(s)	1137
BtsCI	GGATG	1 cut(s)	1327
BtsI	GCAGTG	1 cut(s)	813
BtsIMutI	CAGTG	4 cut(s)	310, 813, 976, 1375
Cac8I	GCNNGC	4 cut(s)	25, 402, 537, 1134
CaiI	CAGNNNCTG	1 cut(s)	797
CciI	TCATGA	1 cut(s)	1375
CfoI	GCGC	1 cut(s)	371
Cfr10I	RCCGGY	1 cut(s)	604
Cfr13I	GGNCC	3 cut(s)	996, 1275, 1453
ClaI	ATCGAT	1 cut(s)	1513
Csp6I	GTAC	2 cut(s)	1075, 1209
CviAII	CATG	9 cut(s)	48, 668, 926, 938, 1065, 1095, 1106, 1138, 1376
CviQI	GTAC	2 cut(s)	1075, 1209
DdeI	CTNAG	5 cut(s)	617, 662, 790, 1113, 1482
DpnI	GATC	7 cut(s)	176, 519, 585, 766, 899, 1512, 1520
DpnII	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
EaeI	YGGCCR	3 cut(s)	450, 602, 1134
Eam1104I	CTCTTC	2 cut(s)	309, 1299
EarI	CTCTTC	2 cut(s)	309, 1299
Ecl136II	GAGCTC	1 cut(s)	294
Eco130I	CCWWGG	2 cut(s)	337, 1137
Eco24I	GRGCYC	2 cut(s)	296, 1089
Eco31I	GGTCTC	1 cut(s)	91
Eco53kI	GAGCTC	1 cut(s)	294
Eco57I	CTGAAG	1 cut(s)	875
EcoICRI	GAGCTC	1 cut(s)	294
EcoRI	GAATTC	1 cut(s)	1039
EcoRII	CCWGG	3 cut(s)	359, 1271, 1277
EcoT14I	CCWWGG	2 cut(s)	337, 1137
EcoT38I	GRGCYC	2 cut(s)	296, 1089
ErhI	CCWWGG	2 cut(s)	337, 1137
FaeI	CATG	9 cut(s)	51, 671, 929, 941, 1068, 1098, 1109, 1141, 1379
FaqI	GGGAC	1 cut(s)	1500
FatI	CATG	9 cut(s)	47, 667, 925, 937, 1064, 1094, 1105, 1137, 1375
FbaI	TGATCA	1 cut(s)	583
Fnu4HI	GCNGC	6 cut(s)	21, 194, 405, 563, 1165, 1479
FokI	GGATG	1 cut(s)	1334
FriOI	GRGCYC	2 cut(s)	296, 1089
Fsp4HI	GCNGC	6 cut(s)	21, 194, 405, 563, 1165, 1479
FspBI	CTAG	3 cut(s)	197, 917, 1289
GlaI	GCGC	1 cut(s)	370
GluI	GCNGC	6 cut(s)	21, 194, 405, 563, 1165, 1479
GsuI	CTGGAG	2 cut(s)	27, 1469
HaeIII	GGCC	6 cut(s)	452, 604, 997, 1136, 1277, 1454
HapII	CCGG	2 cut(s)	605, 1304
HhaI	GCGC	1 cut(s)	371
Hin1II	CATG	9 cut(s)	51, 671, 929, 941, 1068, 1098, 1109, 1141, 1379
Hin6I	GCGC	1 cut(s)	369
HinP1I	GCGC	1 cut(s)	369
HindIII	AAGCTT	2 cut(s)	262, 887
HinfI	GANTC	3 cut(s)	272, 591, 672
HpaII	CCGG	2 cut(s)	605, 1304
HphI	GGTGA	3 cut(s)	281, 424, 1350
Hpy166II	GTNNAC	2 cut(s)	1233, 1351
Hpy188I	TCNGA	7 cut(s)	184, 618, 665, 1038, 1299, 1417, 1523
Hpy188III	TCNNGA	6 cut(s)	167, 515, 642, 715, 893, 1376
Hpy8I	GTNNAC	2 cut(s)	1233, 1351
Hpy99I	CGWCG	1 cut(s)	1421
HpyAV	CCTTC	6 cut(s)	142, 445, 519, 689, 736, 1371
HpyCH4III	ACNGT	4 cut(s)	64, 223, 305, 1396
HpyCH4IV	ACGT	1 cut(s)	414
HpyF10VI	GCNNNNNNNGC	6 cut(s)	689, 921, 964, 1003, 1379, 1460
HpyF3I	CTNAG	5 cut(s)	617, 662, 790, 1113, 1482
HpySE526I	ACGT	1 cut(s)	414
Hsp92II	CATG	9 cut(s)	51, 671, 929, 941, 1068, 1098, 1109, 1141, 1379
HspAI	GCGC	1 cut(s)	369
Ksp22I	TGATCA	1 cut(s)	583
Kzo9I	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
LguI	GCTCTTC	1 cut(s)	1299
LmnI	GCTCC	3 cut(s)	299, 469, 950
Lsp1109I	GCAGC	4 cut(s)	7, 180, 391, 1490
LweI	GCATC	5 cut(s)	180, 261, 855, 1312, 1411
MaeI	CTAG	3 cut(s)	197, 917, 1289
MaeII	ACGT	1 cut(s)	414
MaeIII	GTNAC	3 cut(s)	971, 1171, 1195
MalI	GATC	7 cut(s)	176, 519, 585, 766, 899, 1512, 1520
MboI	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
MboII	GAAGA	6 cut(s)	272, 326, 741, 802, 1051, 1286
MfeI	CAATTG	1 cut(s)	237
MflI	RGATCY	1 cut(s)	1518
MhlI	GDGCHC	4 cut(s)	296, 402, 1089, 1375
MlsI	TGGCCA	2 cut(s)	452, 1136
MluNI	TGGCCA	2 cut(s)	452, 1136
MmeI	TCCRAC	3 cut(s)	557, 1299, 1440
Mox20I	TGGCCA	2 cut(s)	452, 1136
MroXI	GAANNNNTTC	1 cut(s)	346
MscI	TGGCCA	2 cut(s)	452, 1136
MseI	TTAA	3 cut(s)	389, 630, 990
MslI	CAYNNNNRTG	2 cut(s)	306, 1202
Msp20I	TGGCCA	2 cut(s)	452, 1136
MspA1I	CMGCKG	1 cut(s)	1481
MspI	CCGG	2 cut(s)	605, 1304
MspR9I	CCNGG	4 cut(s)	361, 1273, 1279, 1304
MunI	CAATTG	1 cut(s)	237
Mva1269I	GAATGC	1 cut(s)	1055
MvaI	CCWGG	3 cut(s)	361, 1273, 1279
MvnI	CGCG	1 cut(s)	371
MwoI	GCNNNNNNNGC	6 cut(s)	689, 921, 964, 1003, 1379, 1460
NciI	CCSGG	1 cut(s)	1304
NcoI	CCATGG	1 cut(s)	1137
NdeII	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
NlaIII	CATG	9 cut(s)	51, 671, 929, 941, 1068, 1098, 1109, 1141, 1379
NlaIV	GGNNCC	3 cut(s)	846, 998, 1301
NmuCI	GTSAC	3 cut(s)	971, 1171, 1195
PagI	TCATGA	1 cut(s)	1375
PciSI	GCTCTTC	1 cut(s)	1299
PctI	GAATGC	1 cut(s)	1055
PdmI	GAANNNNTTC	1 cut(s)	346
PfeI	GAWTC	3 cut(s)	272, 591, 672
PkrI	GCNGC	6 cut(s)	22, 195, 406, 564, 1166, 1480
Psp124BI	GAGCTC	1 cut(s)	296
Psp6I	CCWGG	3 cut(s)	359, 1271, 1277
PspGI	CCWGG	3 cut(s)	359, 1271, 1277
PspN4I	GGNNCC	3 cut(s)	846, 998, 1301
PspPI	GGNCC	3 cut(s)	996, 1275, 1453
PstI	CTGCAG	1 cut(s)	820
PstNI	CAGNNNCTG	1 cut(s)	797
PsuI	RGATCY	1 cut(s)	1518
PvuII	CAGCTG	1 cut(s)	1481
RsaI	GTAC	2 cut(s)	1076, 1210
RsaNI	GTAC	2 cut(s)	1075, 1209
RseI	CAYNNNNRTG	2 cut(s)	306, 1202
SacI	GAGCTC	1 cut(s)	296
SapI	GCTCTTC	1 cut(s)	1299
SaqAI	TTAA	3 cut(s)	389, 630, 990
SatI	GCNGC	6 cut(s)	21, 194, 405, 563, 1165, 1479
Sau3AI	GATC	7 cut(s)	174, 517, 583, 764, 897, 1510, 1518
Sau96I	GGNCC	3 cut(s)	996, 1275, 1453
ScrFI	CCNGG	4 cut(s)	361, 1273, 1279, 1304
SduI	GDGCHC	4 cut(s)	296, 402, 1089, 1375
SfaNI	GCATC	5 cut(s)	180, 261, 855, 1312, 1411
SfcI	CTRYAG	2 cut(s)	54, 816
SmiMI	CAYNNNNRTG	2 cut(s)	306, 1202
SsiI	CCGC	2 cut(s)	562, 1164
SspMI	CTAG	3 cut(s)	197, 917, 1289
SstI	GAGCTC	1 cut(s)	296
StyD4I	CCNGG	4 cut(s)	359, 1271, 1277, 1302
StyI	CCWWGG	2 cut(s)	337, 1137
TaaI	ACNGT	4 cut(s)	64, 223, 305, 1396
TaiI	ACGT	1 cut(s)	417
TaqI	TCGA	2 cut(s)	312, 1513
TauI	GCSGC	2 cut(s)	565, 1167
TfiI	GAWTC	3 cut(s)	272, 591, 672
Tru1I	TTAA	3 cut(s)	389, 630, 990
Tru9I	TTAA	3 cut(s)	389, 630, 990
TscAI	CASTG	4 cut(s)	310, 820, 976, 1375
TseFI	GTSAC	3 cut(s)	971, 1171, 1195
TseI	GCWGC	4 cut(s)	20, 193, 404, 1478
Tsp45I	GTSAC	3 cut(s)	971, 1171, 1195
TspGWI	ACGGA	4 cut(s)	587, 603, 846, 1401
TspRI	CASTG	4 cut(s)	310, 820, 976, 1375
XapI	RAATTY	4 cut(s)	733, 1039, 1079, 1400
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1144
XmnI	GAANNNNTTC	1 cut(s)	346
XspI	CTAG	3 cut(s)	197, 917, 1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.