Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0011539

Physical Location & Seq

Genomic Coordinates Forward (+)

59867871 .. 59871247

Gene Structure 3377 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0442200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGAAGCGACGCAAGTTCGAGCCGGTGCTGTCTCACACTCGGCTGAGATTGCTTCAAATCCTCATGGCCACACTGTTGGTTTACTTTCTAATCACCAGCTTCGAAACCCCGAAAACCGGGTGGGATTCCGGCGAGGAATACGACGCCTCGTTCGAGCTGTTTAGCGACTCGTCGTTGTCCAGAGAGGGGTTAACGGAAGCTTTGAGTAGACCCACTGACCACGGCCCCCGGCTTCGTGATTCCCGAGGGTCGCCGCTTCAGACACCCGAGCGGAGAATGCGGGAGTCAAAGAGAGTCTCGGGTTTGGTTTTCGAGGGGGAGAAGACTCAGTTTGACGGAAATGTGAGTAAGGCGGACGAAGTTTTCGGGCTTCACAAGGCGGCCGTGGAGGCCTGGAGAGTGGGGAAGAAGCTCTGGGCCGAGATTGAGTCCGGCAAAATTGAATTGGACAAGTCCGAGAACCGGTCCGACCCGTGCCCGCATTCGCTTTCGGTAACCGGGTCCGAATTTCACGCCCGGAACCGGGTGATGGTGATCCCGTGTGGACTGACGCTGTGGTCGCACATTACGGTGGTGGGTACGCCGCGGTGGGCCCACCCGGAATATGATCCGAAGATTGCGGTGGTGAAGGAGGGGGAGGAGGCGGTGATGGTTTCGCAGTTTATGATGGAACTGCAGGGGTTGAAGAATGTGGAGGGGGAGGACCCGCCGAGAATATTGCATTTCAATCCGAGATTAAAGGGGGATTGGAGTAACAAGCCGGTGATTGAGCACAACACTTGTTATAGGATGCAATGGGGTTCGGCGCTTCGGTGCGAGGGCTGGCAAGCCCGGGCTGTTGAAGAAACCGTTGATGGACTGGTGAAATGCGAGAAGTGGATTCGTGATGATGATGATCACTCGGAAGAATCAAAGGCCATATGGTGGTTGAACAGGTTGATAGGAAGGACAAATAAGGTGACCATAGACTGGCCGTACCCTTTTGTAGAGGGCAGGATGTTTGTACTCACCTTGAGTGCTGGCTTGGAAGGTTATCATATCAATGTTGATGGTAGACATGTCACTTCTTTCCCTTATCGCACGGGATTTGTTCTTGAGGATGCCACCGGACTGTTTGTAAATGGAGATGTTGATGTGCACTCTGTCTATGCTGCCTCCTTACCTACATCACATCCTAGTTTTGCTCCACAGATGCATCTTGAGATGGTTACTAAGTGGAAGGCTCCACCTCTTCCCTATGGGCATGTGGAGTTGTTCATTGGCATTCTTTCTGCTGGCAACCATTTTGCTGAGCGGATGGCTGTGAGGAAGTCCTGGATGCAGCATAAGTTAGTCAAGTCTTCACGTGTTGTTGCTCGTTTTTTTGTAGCATTGCATGGTAGAAAGGAAGTAAACCTGGAGCTAATGAAAGAAGTAGAGTATTTTGGTGACATTGTTATAGTTCCTTATATGGATAACTATGATCTTGTAGTACTGAAGACCATAGCAATTTGTGAATATGGGGTTAGAACAGTGCCTGCAAAGTACATCATGAAGTGTGATGATGACACCTTTGTCCGGGTGGATGCTGTGCTCAAGGAAGCAAGGAAAGCGCGTAAACATAGAAGCCTATATATAGGAAATTTGAACTACCACCACAAGCCTCTTCGTTATGGTAAATGGGCAGTGACATATGAGGAATGGCCAGAAGAAGATTATCCAACCTATGCAAATGGTCCAGGTTACATCGTCTCCTCCGATGTAGCAAAATTCATCCTATCTGAGTTTGAAAAGAACAAGTTGCGATTGTTCAAGATGGAAGATGTGAGCATGGGAATGTGGGTGGAGCAGTTCAACGATTCTAGACCAGTGGAGTATGTGCACAGTTTGAAGTTCTGCCAACATGGGTGCATTGAAAACTATTACACTGCGCATTACCAATCCCCGAGGCAGATGATATGCATGTGGGGCAAAATGCAGCATAATGGGAAACCCCAATGCTGCAATATGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0016020 GO:0043207 GO:0044464 GO:0045087 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mlo

Protein Analysis

664

Amino Acids

76.52

Weight (kDa)

7.14

Isoelectric Point (pI)

40.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal-bind_lectin	PF00337	176 - 383	1.4e-26	Galactoside-binding lectin
Galactosyl_T	PF01762	431 - 611	2.3e-33	Galactosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011539)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g08460 FvH4_3g08460 FvH4_3g08460
malus_domestica	MD10G1288000.v1.1
prunus_persica	Prupe.4G053700_v2.0.a1 Prupe.4G053700_v2.0.a1
pyrus_communis	pycom10g24100
rosa_chinensis	RchiOBHm_Chr5g0009371
rosa_laevigata	RLG00000031656
rosa_multiflora	Rmu_sc0002711.1_g000020
rosa_roxburghii	Rroxscaffold_1G00066720 Rroxscaffold_1G00067420
rosa_rugosa	Rorug04G0442100 Rorug04G0442200 Rorug04G0442300
rosa_samantha	Rh5AG073000 Rh5BG069400 Rh5CG081800 Rh5DG069600
rosa_wichuraiana	Rw5G006820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	556, 1553
Acc16I	TGCGCA	1 cut(s)	1911
AccB7I	CCANNNNNTGG	2 cut(s)	924, 969
AccBSI	CCGCTC	2 cut(s)	271, 1294
AccI	GTMKAC	2 cut(s)	208, 1054
AccII	CGCG	2 cut(s)	586, 1594
AclWI	GGATC	2 cut(s)	529, 602
AcoI	YGGCCR	4 cut(s)	66, 381, 971, 1682
AcsI	RAATTY	3 cut(s)	506, 1621, 1748
AcuI	CTGAAG	2 cut(s)	242, 1496
AcvI	CACGTG	1 cut(s)	1346
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	5 cut(s)	580, 977, 1005, 1473, 1526
AfiI	CCNNNNNNNGG	9 cut(s)	116, 186, 462, 522, 523, 529, 924, 969, 1557
AflIII	ACRYGT	2 cut(s)	1057, 1345
AgeI	ACCGGT	1 cut(s)	462
AjnI	CCWGG	4 cut(s)	392, 1313, 1395, 1717
AluBI	AGCT	5 cut(s)	99, 157, 200, 412, 1402
AluI	AGCT	5 cut(s)	99, 157, 200, 412, 1402
Alw21I	GWGCWC	4 cut(s)	774, 1140, 1575, 1863
Alw26I	GTCTC	3 cut(s)	36, 301, 1735
Alw44I	GTGCAC	2 cut(s)	1136, 1859
AlwI	GGATC	2 cut(s)	529, 602
AlwNI	CAGNNNCTG	1 cut(s)	1517
Ama87I	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
AoxI	GGCC	9 cut(s)	66, 223, 381, 390, 417, 591, 915, 971, 1682
ApaI	GGGCCC	1 cut(s)	595
ApaLI	GTGCAC	2 cut(s)	1136, 1859
ApeKI	GCWGC	4 cut(s)	1151, 1321, 1957, 1980
ApoI	RAATTY	3 cut(s)	506, 1621, 1748
ArsI	GACNNNNNNTTYG	4 cut(s)	134, 166, 347, 379
AsiGI	ACCGGT	1 cut(s)	462
AspLEI	GCGC	3 cut(s)	808, 1594, 1912
AspS9I	GGNCC	8 cut(s)	224, 417, 465, 501, 591, 592, 703, 1715
AsuC2I	CCSGG	9 cut(s)	118, 229, 499, 517, 524, 599, 832, 833, 1559
AsuII	TTCGAA	1 cut(s)	102
AvaI	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
AvaII	GGWCC	4 cut(s)	465, 501, 703, 1715
BaeGI	GKGCMC	4 cut(s)	479, 595, 1140, 1863
BalI	TGGCCA	2 cut(s)	68, 1684
BanII	GRGCYC	1 cut(s)	595
BbrPI	CACGTG	1 cut(s)	1346
BbsI	GAAGAC	3 cut(s)	329, 1332, 1484
Bbv12I	GWGCWC	4 cut(s)	774, 1140, 1575, 1863
BbvI	GCAGC	4 cut(s)	1138, 1333, 1967, 1969
BccI	CCATC	8 cut(s)	523, 643, 661, 848, 1043, 1198, 1291, 1789
BceAI	ACGGC	3 cut(s)	238, 368, 958
BcgI	CGANNNNNNTGC	2 cut(s)	700, 734
BciT130I	CCWGG	4 cut(s)	394, 1315, 1397, 1719
BclI	TGATCA	1 cut(s)	895
BcnI	CCSGG	9 cut(s)	118, 229, 499, 517, 524, 599, 832, 833, 1559
BcoDI	GTCTC	3 cut(s)	36, 301, 1735
BfaI	CTAG	2 cut(s)	1176, 1842
BfmI	CTRYAG	1 cut(s)	674
BfoI	RGCGCY	1 cut(s)	809
BglI	GCCNNNNNGGC	1 cut(s)	389
BisI	GCNGC	7 cut(s)	254, 381, 584, 1152, 1322, 1958, 1981
BlpI	GCTNAGC	1 cut(s)	1290
BlsI	GCNGC	7 cut(s)	255, 382, 585, 1153, 1323, 1959, 1982
BmcAI	AGTACT	1 cut(s)	1473
Bme18I	GGWCC	4 cut(s)	465, 501, 703, 1715
BmeT110I	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
BmgT120I	GGNCC	8 cut(s)	224, 417, 465, 501, 591, 592, 703, 1715
BmiI	GGNNCC	6 cut(s)	226, 502, 521, 593, 705, 1224
BmsI	GCATC	6 cut(s)	780, 1090, 1182, 1204, 1308, 1555
BpiI	GAAGAC	3 cut(s)	329, 1332, 1484
BpmI	CTGGAG	2 cut(s)	415, 1418
Bpu1102I	GCTNAGC	1 cut(s)	1290
Bpu14I	TTCGAA	1 cut(s)	102
BpuEI	CTTGAG	4 cut(s)	1033, 1115, 1220, 1559
BpuMI	CCSGG	9 cut(s)	118, 229, 499, 517, 524, 599, 832, 833, 1559
BsaAI	YACGTR	1 cut(s)	1346
BsaBI	GATNNNNATC	2 cut(s)	533, 894
BsaHI	GRCGYC	1 cut(s)	144
BsaJI	CCNNGG	7 cut(s)	220, 227, 244, 384, 584, 831, 1925
BsaWI	WCCGGW	2 cut(s)	462, 1106
BsaXI	ACNNNNNCTCC	2 cut(s)	1715, 1745
Bsc4I	CCNNNNNNNGG	9 cut(s)	116, 186, 462, 522, 523, 529, 924, 969, 1557
Bse118I	RCCGGY	3 cut(s)	22, 462, 760
Bse1I	ACTGG	3 cut(s)	864, 974, 1847
Bse3DI	GCAATG	2 cut(s)	800, 1370
Bse8I	GATNNNNATC	2 cut(s)	533, 894
BseBI	CCWGG	4 cut(s)	394, 1315, 1397, 1719
BseDI	CCNNGG	7 cut(s)	220, 227, 244, 384, 584, 831, 1925
BseGI	GGATG	8 cut(s)	795, 1002, 1105, 1171, 1302, 1323, 1570, 1752
BseJI	GATNNNNATC	2 cut(s)	533, 894
BseLI	CCNNNNNNNGG	9 cut(s)	116, 186, 462, 522, 523, 529, 924, 969, 1557
BseMI	GCAATG	2 cut(s)	800, 1370
BseMII	CTCAG	4 cut(s)	35, 341, 1281, 1752
BseNI	ACTGG	3 cut(s)	864, 974, 1847
BseRI	GAGGAG	2 cut(s)	653, 1723
BseSI	GKGCMC	4 cut(s)	479, 595, 1140, 1863
BseX3I	CGGCCG	1 cut(s)	381
BseXI	GCAGC	4 cut(s)	1138, 1333, 1967, 1969
Bsh1236I	CGCG	2 cut(s)	586, 1594
Bsh1285I	CGRYCG	1 cut(s)	384
BshFI	GGCC	9 cut(s)	68, 225, 383, 392, 419, 593, 917, 973, 1684
BshTI	ACCGGT	1 cut(s)	462
BsiEI	CGRYCG	1 cut(s)	384
BsiHKAI	GWGCWC	4 cut(s)	774, 1140, 1575, 1863
BsiHKCI	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
BslI	CCNNNNNNNGG	9 cut(s)	116, 186, 462, 522, 523, 529, 924, 969, 1557
BsmAI	GTCTC	3 cut(s)	36, 301, 1735
BsmBI	CGTCTC	1 cut(s)	1735
BsmI	GAATGC	3 cut(s)	282, 481, 1263
BsnI	GGCC	9 cut(s)	68, 225, 383, 392, 419, 593, 917, 973, 1684
BsoBI	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
Bsp119I	TTCGAA	1 cut(s)	102
Bsp120I	GGGCCC	1 cut(s)	591
Bsp1286I	GDGCHC	6 cut(s)	479, 595, 774, 1140, 1575, 1863
Bsp143I	GATC	4 cut(s)	534, 607, 895, 1462
Bsp1720I	GCTNAGC	1 cut(s)	1290
BspANI	GGCC	9 cut(s)	68, 225, 383, 392, 419, 593, 917, 973, 1684
BspCNI	CTCAG	4 cut(s)	36, 340, 1282, 1753
BspFNI	CGCG	2 cut(s)	586, 1594
BspHI	TCATGA	1 cut(s)	1530
BspLI	GGNNCC	6 cut(s)	226, 502, 521, 593, 705, 1224
BspMAI	CTGCAG	1 cut(s)	678
BspPI	GGATC	2 cut(s)	529, 602
BspT104I	TTCGAA	1 cut(s)	102
BsrBI	CCGCTC	2 cut(s)	271, 1294
BsrDI	GCAATG	2 cut(s)	800, 1370
BsrFI	RCCGGY	3 cut(s)	22, 462, 760
BsrI	ACTGG	3 cut(s)	864, 974, 1847
BssAI	RCCGGY	3 cut(s)	22, 462, 760
BssECI	CCNNGG	7 cut(s)	220, 227, 244, 384, 584, 831, 1925
BssMI	GATC	4 cut(s)	534, 607, 895, 1462
BssNI	GRCGYC	1 cut(s)	144
Bst2UI	CCWGG	4 cut(s)	394, 1315, 1397, 1719
Bst4CI	ACNGT	6 cut(s)	75, 571, 850, 1113, 1513, 1865
Bst6I	CTCTTC	2 cut(s)	1236, 1650
BstACI	GRCGYC	1 cut(s)	144
BstBAI	YACGTR	1 cut(s)	1346
BstBI	TTCGAA	1 cut(s)	102
BstC8I	GCNNGC	6 cut(s)	479, 824, 828, 1021, 1276, 1518
BstDEI	CTNAG	5 cut(s)	44, 327, 1212, 1290, 1761
BstDSI	CCRYGG	3 cut(s)	220, 384, 584
BstEII	GGTNACC	2 cut(s)	493, 958
BstF5I	GGATG	8 cut(s)	795, 1002, 1105, 1171, 1302, 1323, 1570, 1752
BstFNI	CGCG	2 cut(s)	586, 1594
BstH2I	RGCGCY	1 cut(s)	809
BstHHI	GCGC	3 cut(s)	808, 1594, 1912
BstKTI	GATC	4 cut(s)	537, 610, 898, 1465
BstMAI	GTCTC	3 cut(s)	36, 301, 1735
BstMBI	GATC	4 cut(s)	534, 607, 895, 1462
BstMCI	CGRYCG	1 cut(s)	384
BstMWI	GCNNNNNNNGC	6 cut(s)	49, 277, 389, 559, 1589, 1947
BstNI	CCWGG	4 cut(s)	394, 1315, 1397, 1719
BstNSI	RCATGY	3 cut(s)	1061, 1247, 1945
BstPI	GGTNACC	2 cut(s)	493, 958
BstSFI	CTRYAG	1 cut(s)	674
BstSLI	GKGCMC	4 cut(s)	479, 595, 1140, 1863
BstUI	CGCG	2 cut(s)	586, 1594
BstV1I	GCAGC	4 cut(s)	1138, 1333, 1967, 1969
BstV2I	GAAGAC	3 cut(s)	329, 1332, 1484
BstXI	CCANNNNNNTGG	1 cut(s)	76
BstZI	CGGCCG	1 cut(s)	381
BsuRI	GGCC	9 cut(s)	68, 225, 383, 392, 419, 593, 917, 973, 1684
BtgI	CCRYGG	3 cut(s)	220, 384, 584
BtsCI	GGATG	8 cut(s)	795, 1002, 1105, 1171, 1302, 1323, 1570, 1752
BtsI	GCAGTG	2 cut(s)	1671, 1905
BtsIMutI	CAGTG	6 cut(s)	71, 213, 1518, 1671, 1854, 1905
Cac8I	GCNNGC	6 cut(s)	479, 824, 828, 1021, 1276, 1518
CaiI	CAGNNNCTG	1 cut(s)	1517
CciI	TCATGA	1 cut(s)	1530
CfoI	GCGC	3 cut(s)	808, 1594, 1912
Cfr10I	RCCGGY	3 cut(s)	22, 462, 760
Cfr13I	GGNCC	8 cut(s)	224, 417, 465, 501, 591, 592, 703, 1715
Cfr42I	CCGCGG	1 cut(s)	587
Cfr9I	CCCGGG	1 cut(s)	831
CpoI	CGGWCCG	1 cut(s)	465
CseI	GACGC	3 cut(s)	18, 152, 559
Csp6I	GTAC	5 cut(s)	579, 976, 1004, 1472, 1525
CspAI	ACCGGT	1 cut(s)	462
CspI	CGGWCCG	1 cut(s)	465
CviAII	CATG	8 cut(s)	64, 1058, 1244, 1376, 1531, 1810, 1883, 1942
CviQI	GTAC	5 cut(s)	579, 976, 1004, 1472, 1525
DdeI	CTNAG	5 cut(s)	44, 327, 1212, 1290, 1761
DpnI	GATC	4 cut(s)	536, 609, 897, 1464
DpnII	GATC	4 cut(s)	534, 607, 895, 1462
DrdI	GACNNNNNNGTC	2 cut(s)	556, 1553
DseDI	GACNNNNNNGTC	2 cut(s)	556, 1553
EaeI	YGGCCR	4 cut(s)	66, 381, 971, 1682
EagI	CGGCCG	1 cut(s)	381
Eam1104I	CTCTTC	2 cut(s)	1236, 1650
EarI	CTCTTC	2 cut(s)	1236, 1650
EciI	GGCGGA	1 cut(s)	368
EclXI	CGGCCG	1 cut(s)	381
Eco147I	AGGCCT	1 cut(s)	392
Eco24I	GRGCYC	1 cut(s)	595
Eco47I	GGWCC	4 cut(s)	465, 501, 703, 1715
Eco52I	CGGCCG	1 cut(s)	381
Eco57I	CTGAAG	2 cut(s)	242, 1496
Eco72I	CACGTG	1 cut(s)	1346
Eco88I	CYCGRG	5 cut(s)	243, 266, 298, 831, 1924
Eco91I	GGTNACC	2 cut(s)	493, 958
EcoO109I	RGGNCCY	1 cut(s)	703
EcoO65I	GGTNACC	2 cut(s)	493, 958
EcoRII	CCWGG	4 cut(s)	392, 1313, 1395, 1717
EcoT22I	ATGCAT	2 cut(s)	1197, 1943
EcoT38I	GRGCYC	1 cut(s)	595
Esp3I	CGTCTC	1 cut(s)	1735
FaeI	CATG	8 cut(s)	67, 1061, 1247, 1379, 1534, 1813, 1886, 1945
FatI	CATG	8 cut(s)	63, 1057, 1243, 1375, 1530, 1809, 1882, 1941
FauI	CCCGC	3 cut(s)	273, 486, 714
FauNDI	CATATG	2 cut(s)	920, 1672
FbaI	TGATCA	1 cut(s)	895
FblI	GTMKAC	2 cut(s)	208, 1054
Fnu4HI	GCNGC	7 cut(s)	254, 381, 584, 1152, 1322, 1958, 1981
FokI	GGATG	8 cut(s)	802, 1009, 1112, 1158, 1309, 1330, 1577, 1739
FriOI	GRGCYC	1 cut(s)	595
Fsp4HI	GCNGC	7 cut(s)	254, 381, 584, 1152, 1322, 1958, 1981
FspBI	CTAG	2 cut(s)	1176, 1842
FspI	TGCGCA	1 cut(s)	1911
GlaI	GCGC	3 cut(s)	807, 1593, 1911
GluI	GCNGC	7 cut(s)	254, 381, 584, 1152, 1322, 1958, 1981
GsuI	CTGGAG	2 cut(s)	415, 1418
HaeII	RGCGCY	1 cut(s)	809
HaeIII	GGCC	9 cut(s)	68, 225, 383, 392, 419, 593, 917, 973, 1684
HgaI	GACGC	3 cut(s)	18, 152, 559
HhaI	GCGC	3 cut(s)	808, 1594, 1912
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	8 cut(s)	67, 1061, 1247, 1379, 1534, 1813, 1886, 1945
Hin6I	GCGC	3 cut(s)	806, 1592, 1910
HinP1I	GCGC	3 cut(s)	806, 1592, 1910
HincII	GTYRAC	1 cut(s)	192
HindII	GTYRAC	1 cut(s)	192
HindIII	AAGCTT	1 cut(s)	198
HpaI	GTTAAC	1 cut(s)	192
Hpy166II	GTNNAC	9 cut(s)	82, 192, 209, 545, 1055, 1138, 1393, 1598, 1861
Hpy188I	TCNGA	9 cut(s)	261, 457, 469, 505, 612, 732, 904, 1738, 1762
Hpy188III	TCNNGA	9 cut(s)	180, 236, 243, 884, 1094, 1199, 1531, 1792, 1842
Hpy8I	GTNNAC	9 cut(s)	82, 192, 209, 545, 1055, 1138, 1393, 1598, 1861
Hpy99I	CGWCG	3 cut(s)	12, 146, 175
HpyAV	CCTTC	4 cut(s)	622, 939, 1022, 1213
HpyCH4III	ACNGT	6 cut(s)	75, 571, 850, 1113, 1513, 1865
HpyCH4IV	ACGT	1 cut(s)	1345
HpyF10VI	GCNNNNNNNGC	6 cut(s)	49, 277, 389, 559, 1589, 1947
HpyF3I	CTNAG	5 cut(s)	44, 327, 1212, 1290, 1761
HpySE526I	ACGT	1 cut(s)	1345
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	8 cut(s)	67, 1061, 1247, 1379, 1534, 1813, 1886, 1945
HspAI	GCGC	3 cut(s)	806, 1592, 1910
Ksp22I	TGATCA	1 cut(s)	895
KspAI	GTTAAC	1 cut(s)	192
KspI	CCGCGG	1 cut(s)	587
Kzo9I	GATC	4 cut(s)	534, 607, 895, 1462
LmnI	GCTCC	4 cut(s)	1189, 1228, 1399, 1825
Lsp1109I	GCAGC	4 cut(s)	1138, 1333, 1967, 1969
LweI	GCATC	6 cut(s)	780, 1090, 1182, 1204, 1308, 1555
MaeI	CTAG	2 cut(s)	1176, 1842
MaeII	ACGT	1 cut(s)	1345
MaeIII	GTNAC	8 cut(s)	493, 752, 958, 1060, 1207, 1427, 1666, 1721
MalI	GATC	4 cut(s)	536, 609, 897, 1464
MbiI	CCGCTC	2 cut(s)	271, 1294
MboI	GATC	4 cut(s)	534, 607, 895, 1462
MhlI	GDGCHC	6 cut(s)	479, 595, 774, 1140, 1575, 1863
MlsI	TGGCCA	2 cut(s)	68, 1684
MluCI	AATT	6 cut(s)	438, 443, 506, 1488, 1621, 1748
MluNI	TGGCCA	2 cut(s)	68, 1684
MlyI	GAGTC	5 cut(s)	161, 293, 303, 319, 437
MmeI	TCCRAC	2 cut(s)	492, 1724
Mox20I	TGGCCA	2 cut(s)	68, 1684
Mph1103I	ATGCAT	2 cut(s)	1197, 1943
MscI	TGGCCA	2 cut(s)	68, 1684
MseI	TTAA	2 cut(s)	191, 737
MslI	CAYNNNNRTG	3 cut(s)	569, 1041, 1814
Msp20I	TGGCCA	2 cut(s)	68, 1684
MspA1I	CMGCKG	1 cut(s)	586
Mva1269I	GAATGC	3 cut(s)	282, 481, 1263
MvaI	CCWGG	4 cut(s)	394, 1315, 1397, 1719
MvnI	CGCG	2 cut(s)	586, 1594
MwoI	GCNNNNNNNGC	6 cut(s)	49, 277, 389, 559, 1589, 1947
NciI	CCSGG	9 cut(s)	118, 229, 499, 517, 524, 599, 832, 833, 1559
NdeI	CATATG	2 cut(s)	920, 1672
NdeII	GATC	4 cut(s)	534, 607, 895, 1462
NlaIII	CATG	8 cut(s)	67, 1061, 1247, 1379, 1534, 1813, 1886, 1945
NlaIV	GGNNCC	6 cut(s)	226, 502, 521, 593, 705, 1224
NmeAIII	GCCGAG	3 cut(s)	19, 445, 735
NmuCI	GTSAC	4 cut(s)	958, 1060, 1427, 1666
NsbI	TGCGCA	1 cut(s)	1911
NsiI	ATGCAT	2 cut(s)	1197, 1943
NspI	RCATGY	3 cut(s)	1061, 1247, 1945
NspV	TTCGAA	1 cut(s)	102
PagI	TCATGA	1 cut(s)	1530
PceI	AGGCCT	1 cut(s)	392
PciI	ACATGT	1 cut(s)	1057
PcsI	WCGNNNNNNNCGW	3 cut(s)	150, 241, 1734
PctI	GAATGC	3 cut(s)	282, 481, 1263
PfeI	GAWTC	5 cut(s)	125, 239, 880, 908, 1838
PflMI	CCANNNNNTGG	2 cut(s)	924, 969
PfoI	TCCNGGA	1 cut(s)	1313
PinAI	ACCGGT	1 cut(s)	462
PkrI	GCNGC	7 cut(s)	255, 382, 585, 1153, 1323, 1959, 1982
PleI	GAGTC	5 cut(s)	161, 292, 302, 319, 436
PmaCI	CACGTG	1 cut(s)	1346
PmlI	CACGTG	1 cut(s)	1346
PpsI	GAGTC	5 cut(s)	161, 292, 302, 319, 436
Ppu21I	YACGTR	1 cut(s)	1346
PpuMI	RGGWCCY	1 cut(s)	703
PscI	ACATGT	1 cut(s)	1057
Psp5II	RGGWCCY	1 cut(s)	703
Psp6I	CCWGG	4 cut(s)	392, 1313, 1395, 1717
PspCI	CACGTG	1 cut(s)	1346
PspEI	GGTNACC	2 cut(s)	493, 958
PspGI	CCWGG	4 cut(s)	392, 1313, 1395, 1717
PspN4I	GGNNCC	6 cut(s)	226, 502, 521, 593, 705, 1224
PspOMI	GGGCCC	1 cut(s)	591
PspPI	GGNCC	8 cut(s)	224, 417, 465, 501, 591, 592, 703, 1715
PspPPI	RGGWCCY	1 cut(s)	703
PstI	CTGCAG	1 cut(s)	678
PstNI	CAGNNNCTG	1 cut(s)	1517
RsaI	GTAC	5 cut(s)	580, 977, 1005, 1473, 1526
RsaNI	GTAC	5 cut(s)	579, 976, 1004, 1472, 1525
RseI	CAYNNNNRTG	3 cut(s)	569, 1041, 1814
Rsr2I	CGGWCCG	1 cut(s)	465
RsrII	CGGWCCG	1 cut(s)	465
SacII	CCGCGG	1 cut(s)	587
SaqAI	TTAA	2 cut(s)	191, 737
SatI	GCNGC	7 cut(s)	254, 381, 584, 1152, 1322, 1958, 1981
Sau3AI	GATC	4 cut(s)	534, 607, 895, 1462
Sau96I	GGNCC	8 cut(s)	224, 417, 465, 501, 591, 592, 703, 1715
ScaI	AGTACT	1 cut(s)	1473
SchI	GAGTC	5 cut(s)	161, 293, 303, 319, 437
SduI	GDGCHC	6 cut(s)	479, 595, 774, 1140, 1575, 1863
SfaNI	GCATC	6 cut(s)	780, 1090, 1182, 1204, 1308, 1555
SfcI	CTRYAG	1 cut(s)	674
SfiI	GGCCNNNNNGGCC	1 cut(s)	389
Sfr303I	CCGCGG	1 cut(s)	587
SfuI	TTCGAA	1 cut(s)	102
SgrBI	CCGCGG	1 cut(s)	587
SinI	GGWCC	4 cut(s)	465, 501, 703, 1715
SmaI	CCCGGG	1 cut(s)	833
SmiMI	CAYNNNNRTG	3 cut(s)	569, 1041, 1814
SmlI	CTYRAG	4 cut(s)	1012, 1094, 1199, 1574
SmoI	CTYRAG	4 cut(s)	1012, 1094, 1199, 1574
SrfI	GCCCGGGC	1 cut(s)	833
Sse9I	AATT	6 cut(s)	438, 443, 506, 1488, 1621, 1748
SseBI	AGGCCT	1 cut(s)	392
SspI	AATATT	1 cut(s)	717
SspMI	CTAG	2 cut(s)	1176, 1842
StuI	AGGCCT	1 cut(s)	392
TaaI	ACNGT	6 cut(s)	75, 571, 850, 1113, 1513, 1865
TaiI	ACGT	1 cut(s)	1348
TaqI	TCGA	4 cut(s)	18, 102, 153, 312
TasI	AATT	6 cut(s)	438, 443, 506, 1488, 1621, 1748
TatI	WGTACW	3 cut(s)	1003, 1471, 1524
TauI	GCSGC	3 cut(s)	256, 383, 586
TfiI	GAWTC	5 cut(s)	125, 239, 880, 908, 1838
Tru1I	TTAA	2 cut(s)	191, 737
Tru9I	TTAA	2 cut(s)	191, 737
TscAI	CASTG	6 cut(s)	78, 220, 1518, 1671, 1854, 1912
TseFI	GTSAC	4 cut(s)	958, 1060, 1427, 1666
TseI	GCWGC	4 cut(s)	1151, 1321, 1957, 1980
Tsp45I	GTSAC	4 cut(s)	958, 1060, 1427, 1666
TspDTI	ATGAA	5 cut(s)	17, 1246, 1421, 1547, 1741
TspGWI	ACGGA	2 cut(s)	209, 351
TspMI	CCCGGG	1 cut(s)	831
TspRI	CASTG	6 cut(s)	78, 220, 1518, 1671, 1854, 1912
Van91I	CCANNNNNTGG	2 cut(s)	924, 969
VneI	GTGCAC	2 cut(s)	1136, 1859
VpaK11BI	GGWCC	4 cut(s)	465, 501, 703, 1715
XapI	RAATTY	3 cut(s)	506, 1621, 1748
XbaI	TCTAGA	1 cut(s)	1841
XceI	RCATGY	3 cut(s)	1061, 1247, 1945
XmaI	CCCGGG	1 cut(s)	831
XmiI	GTMKAC	2 cut(s)	208, 1054
XspI	CTAG	2 cut(s)	1176, 1842
ZrmI	AGTACT	1 cut(s)	1473
Zsp2I	ATGCAT	2 cut(s)	1197, 1943

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.