Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011539

Physical Location & Seq

Genomic Coordinates Reverse (-)

7135900 .. 7139385

Gene Structure 3486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031656

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGCCGGAGAGGAAGTCAGTAGTGAAAGCACCACACTAGAATCAACACCAACATGGGCTGTCGCCACCGTCTGCTTCGTTTTGATTTTCATCTCCATTCTCATCGAGCATTTGCTTCATCTTTTAGCCCATTATTTCAACAAGATAAGGAGGAAGTCTCTCATTCTCGCTCTTGATAAGATCAAATCAGAGTTGATGTTGCTGGGGTTCATTTCTTTGCTGCTAACCGTGGGGCAAAAGCCGATTGCCAATATCTGCATCCCGAAATCTGCCGGAGAGTCTTTTCTTCCTTGCCAGAGCTACACCTTGAGTGAAGATATTGTGGAGGAAACGAAATGTGCAGAGCAGGGAAAGGTTTCATTGATGTCGAGGTCCGGTGCCAATGAGCTTCAATACTTTATTTTTGTGCTCGCACTGTTCCACGTCTTGTCTTGCGTTCTCACTTCCACTCTTGGTTTTGCCAAGATGAAGAGCTGGCAGTCTTGGGAGGCAGAAACCAGAACCATGGAGTATCAATTTTCTTATGATCCAAGGAGATTTCAGCTGGCACACCAAACCTCATTTGGGAAGAGACATTTGAAATGTTGGAGTGACCACAGAATACTCCGTTGGCCGGCCTGTTTTGCAAGGCAATTCTTCAGTTCTGTTTCCAAAGTGGATTATTTCACTCTTCGACACGGATTCATAACATTAATAAATGTAAGTCTGACTTGGATAGCTAACACAATAATTCACTGTGTCTCTGCAGGATTTTATAATTATCTATGGGTACCGTCCATTCCTTTAGTGATGCTATTGCTTGTGGGAACAAAGCTACAAGGCATCATAACTAAGATGTGTGTAGATAGCCATGATAAATCCCATGTTGTAAGAGGGACTTTGCTTGTGAAGCCCAGTGACCACTTTTTCTGGTTTGGCTGGCCCAAATTGCTTCTCCATATTTTGCACTTCACACTGTATCAGAACTCATTTCAATTGGCATTCTTCACTTGGACTTGGTACAAATTTGGTTTGAAATCATGCTTCCACCGAGAAACTGAGGATATAGTAATACGGCTGGCCATGGGCATCATGGTACAAATCCTGTGTGGCTATGTAACTCTACCTCTTTACGCATTGGTCACTCAGATGGGTACGTCGATGAGAAAAGCTGTCTTCACCGAGACAGTGGTTACCGGACTGAAAAAATGGAGAGGAAGAGCCAGGAAGAACCTTGCTCTTAAGAGCTCGGCACTAATGTCTTTGGATGCTTCCCTTCAAACCTCACTCTCTCTAGACACTACACTTTCTTTCAATCACATTGATGCTTCGGCTTCTTTCTCCACCGTGGATTTCGACGCTGAATATGTAGCAGTAGAAATAATGGTCAATGCTGGAAATGTTGTACAGGGAAAACAAGTACCGGAGGCGGAGTATCGAGACCAGAAGCCTGGACCATCATTTGATGGTTTTGATTTGAGTAGTAGTAAAAAATCTCAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0016020 GO:0043207 GO:0044464 GO:0045087 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mlo

Protein Analysis

495

Amino Acids

56.05

Weight (kDa)

8.97

Isoelectric Point (pI)

36.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mlo	PF03094	11 - 102	1.1e-37	Mlo family
Mlo	PF03094	109 - 231	5.6e-49	Mlo family
Mlo	PF03094	237 - 413	3.2e-70	Mlo family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011539)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g08460 FvH4_3g08460 FvH4_3g08460
malus_domestica	MD10G1288000.v1.1
prunus_persica	Prupe.4G053700_v2.0.a1 Prupe.4G053700_v2.0.a1
pyrus_communis	pycom10g24100
rosa_chinensis	RchiOBHm_Chr5g0009371
rosa_laevigata	RLG00000031656
rosa_multiflora	Rmu_sc0002711.1_g000020
rosa_roxburghii	Rroxscaffold_1G00066720 Rroxscaffold_1G00067420
rosa_rugosa	Rorug04G0442100 Rorug04G0442200 Rorug04G0442300
rosa_samantha	Rh5AG073000 Rh5BG069400 Rh5CG081800 Rh5DG069600
rosa_wichuraiana	Rw5G006820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	756
Acc65I	GGTACC	1 cut(s)	769
AccB1I	GGYRCC	2 cut(s)	377, 769
AciI	CCGC	1 cut(s)	1409
AclWI	GGATC	1 cut(s)	521
AcoI	YGGCCR	3 cut(s)	3, 611, 1059
AcsI	RAATTY	1 cut(s)	1004
AcuI	CTGAAG	1 cut(s)	622
AfaI	GTAC	6 cut(s)	771, 1001, 1077, 1135, 1386, 1401
AfiI	CCNNNNNNNGG	2 cut(s)	564, 613
AflII	CTTAAG	1 cut(s)	1220
AgsI	TTSAA	7 cut(s)	139, 392, 580, 974, 1015, 1259, 1294
AjiI	CACGTC	1 cut(s)	424
AjnI	CCWGG	2 cut(s)	1202, 1429
AjuI	GAANNNNNNNTTGG	2 cut(s)	592, 624
AluBI	AGCT	8 cut(s)	300, 388, 474, 544, 719, 814, 1151, 1227
AluI	AGCT	8 cut(s)	300, 388, 474, 544, 719, 814, 1151, 1227
Alw21I	GWGCWC	2 cut(s)	411, 1229
Alw26I	GTCTC	5 cut(s)	162, 565, 745, 1157, 1413
AlwI	GGATC	1 cut(s)	521
AoxI	GGCC	5 cut(s)	3, 611, 615, 920, 1059
ApeKI	GCWGC	1 cut(s)	220
ApoI	RAATTY	1 cut(s)	1004
AseI	ATTAAT	1 cut(s)	692
Asp700I	GAANNNNTTC	1 cut(s)	355
Asp718I	GGTACC	1 cut(s)	769
AspS9I	GGNCC	3 cut(s)	372, 921, 1433
AsuHPI	GGTGA	1 cut(s)	1150
AvaII	GGWCC	2 cut(s)	372, 1433
BalI	TGGCCA	1 cut(s)	1061
BanI	GGYRCC	2 cut(s)	377, 769
BanII	GRGCYC	1 cut(s)	1229
BbsI	GAAGAC	1 cut(s)	1147
Bbv12I	GWGCWC	2 cut(s)	411, 1229
BbvI	GCAGC	1 cut(s)	207
BccI	CCATC	3 cut(s)	1123, 1439, 1444
BceAI	ACGGC	1 cut(s)	1070
BciT130I	CCWGG	2 cut(s)	1204, 1431
BcoDI	GTCTC	5 cut(s)	162, 565, 745, 1157, 1413
BfaI	CTAG	2 cut(s)	38, 1274
BfmI	CTRYAG	1 cut(s)	744
BfrI	CTTAAG	1 cut(s)	1220
BisI	GCNGC	1 cut(s)	221
BlsI	GCNGC	1 cut(s)	222
Bme1390I	CCNGG	2 cut(s)	1204, 1431
Bme18I	GGWCC	2 cut(s)	372, 1433
BmgBI	CACGTC	1 cut(s)	424
BmgT120I	GGNCC	3 cut(s)	372, 921, 1433
BmiI	GGNNCC	2 cut(s)	379, 771
BmrFI	CCNGG	2 cut(s)	1204, 1431
BmrI	ACTGGG	1 cut(s)	888
BmsI	GCATC	6 cut(s)	267, 780, 831, 1077, 1237, 1294
BmuI	ACTGGG	1 cut(s)	888
BpiI	GAAGAC	1 cut(s)	1147
BplI	GAGNNNNNCTC	2 cut(s)	142, 174
BpuEI	CTTGAG	1 cut(s)	328
BsaBI	GATNNNNATC	1 cut(s)	89
BsaI	GGTCTC	1 cut(s)	1413
BsaJI	CCNNGG	5 cut(s)	228, 504, 530, 1062, 1326
BsaWI	WCCGGW	3 cut(s)	374, 1175, 1402
BsaXI	ACNNNNNCTCC	3 cut(s)	30, 1397, 1427
Bsc4I	CCNNNNNNNGG	2 cut(s)	564, 613
Bse118I	RCCGGY	1 cut(s)	613
Bse1I	ACTGG	1 cut(s)	894
Bse8I	GATNNNNATC	1 cut(s)	89
BseBI	CCWGG	2 cut(s)	1204, 1431
BseDI	CCNNGG	5 cut(s)	228, 504, 530, 1062, 1326
BseGI	GGATG	2 cut(s)	258, 1252
BseJI	GATNNNNATC	1 cut(s)	89
BseLI	CCNNNNNNNGG	2 cut(s)	564, 613
BseMII	CTCAG	2 cut(s)	1029, 1139
BseNI	ACTGG	1 cut(s)	894
BseXI	GCAGC	1 cut(s)	207
BseYI	CCCAGC	1 cut(s)	202
BsgI	GTGCAG	1 cut(s)	360
BshFI	GGCC	5 cut(s)	5, 613, 617, 922, 1061
BshNI	GGYRCC	2 cut(s)	377, 769
BsiHKAI	GWGCWC	2 cut(s)	411, 1229
BsiSI	CCGG	6 cut(s)	6, 273, 375, 614, 1176, 1403
BslFI	GGGAC	1 cut(s)	889
BslI	CCNNNNNNNGG	2 cut(s)	564, 613
BsmAI	GTCTC	5 cut(s)	162, 565, 745, 1157, 1413
BsmFI	GGGAC	1 cut(s)	889
BsmI	GAATGC	1 cut(s)	980
BsnI	GGCC	5 cut(s)	5, 613, 617, 922, 1061
Bso31I	GGTCTC	1 cut(s)	1413
Bsp1286I	GDGCHC	2 cut(s)	411, 1229
Bsp1407I	TGTACA	1 cut(s)	1384
Bsp143I	GATC	2 cut(s)	180, 526
Bsp19I	CCATGG	2 cut(s)	504, 1062
BspACI	CCGC	1 cut(s)	1409
BspANI	GGCC	5 cut(s)	5, 613, 617, 922, 1061
BspCNI	CTCAG	2 cut(s)	1030, 1138
BspLI	GGNNCC	2 cut(s)	379, 771
BspMAI	CTGCAG	1 cut(s)	748
BspPI	GGATC	1 cut(s)	521
BspQI	GCTCTTC	2 cut(s)	464, 1192
BspT107I	GGYRCC	2 cut(s)	377, 769
BspTI	CTTAAG	1 cut(s)	1220
BspTNI	GGTCTC	1 cut(s)	1413
BsrFI	RCCGGY	1 cut(s)	613
BsrGI	TGTACA	1 cut(s)	1384
BsrI	ACTGG	1 cut(s)	894
BssAI	RCCGGY	1 cut(s)	613
BssECI	CCNNGG	5 cut(s)	228, 504, 530, 1062, 1326
BssMI	GATC	2 cut(s)	180, 526
BssT1I	CCWWGG	3 cut(s)	504, 530, 1062
Bst2UI	CCWGG	2 cut(s)	1204, 1431
Bst4CI	ACNGT	8 cut(s)	70, 229, 417, 737, 774, 957, 1168, 1327
Bst6I	CTCTTC	4 cut(s)	464, 563, 675, 1192
BstAFI	CTTAAG	1 cut(s)	1220
BstAUI	TGTACA	1 cut(s)	1384
BstC8I	GCNNGC	6 cut(s)	411, 476, 546, 615, 920, 1059
BstDEI	CTNAG	3 cut(s)	831, 1038, 1125
BstDSI	CCRYGG	4 cut(s)	228, 504, 1062, 1326
BstEII	GGTNACC	1 cut(s)	1171
BstF5I	GGATG	2 cut(s)	258, 1252
BstKTI	GATC	2 cut(s)	183, 529
BstMAI	GTCTC	5 cut(s)	162, 565, 745, 1157, 1413
BstMBI	GATC	2 cut(s)	180, 526
BstMWI	GCNNNNNNNGC	3 cut(s)	623, 889, 928
BstNI	CCWGG	2 cut(s)	1204, 1431
BstPI	GGTNACC	1 cut(s)	1171
BstSCI	CCNGG	2 cut(s)	1202, 1429
BstSFI	CTRYAG	1 cut(s)	744
BstV1I	GCAGC	1 cut(s)	207
BstV2I	GAAGAC	1 cut(s)	1147
BstXI	CCANNNNNNTGG	2 cut(s)	505, 1430
BsuRI	GGCC	5 cut(s)	5, 613, 617, 922, 1061
BtgI	CCRYGG	4 cut(s)	228, 504, 1062, 1326
BtrI	CACGTC	1 cut(s)	424
BtsCI	GGATG	2 cut(s)	258, 1252
BtsIMutI	CAGTG	5 cut(s)	413, 733, 901, 953, 1173
Cac8I	GCNNGC	6 cut(s)	411, 476, 546, 615, 920, 1059
Cfr10I	RCCGGY	1 cut(s)	613
Cfr13I	GGNCC	3 cut(s)	372, 921, 1433
CseI	GACGC	1 cut(s)	1346
Csp6I	GTAC	6 cut(s)	770, 1000, 1076, 1134, 1385, 1400
CviAII	CATG	7 cut(s)	54, 505, 851, 863, 1020, 1063, 1072
CviQI	GTAC	6 cut(s)	770, 1000, 1076, 1134, 1385, 1400
DdeI	CTNAG	3 cut(s)	831, 1038, 1125
DpnI	GATC	2 cut(s)	182, 528
DpnII	GATC	2 cut(s)	180, 526
EaeI	YGGCCR	3 cut(s)	3, 611, 1059
Eam1104I	CTCTTC	4 cut(s)	464, 563, 675, 1192
EarI	CTCTTC	4 cut(s)	464, 563, 675, 1192
EciI	GGCGGA	1 cut(s)	1424
Ecl136II	GAGCTC	1 cut(s)	1227
Eco130I	CCWWGG	3 cut(s)	504, 530, 1062
Eco24I	GRGCYC	1 cut(s)	1229
Eco31I	GGTCTC	1 cut(s)	1413
Eco47I	GGWCC	2 cut(s)	372, 1433
Eco53kI	GAGCTC	1 cut(s)	1227
Eco57I	CTGAAG	1 cut(s)	622
Eco91I	GGTNACC	1 cut(s)	1171
EcoICRI	GAGCTC	1 cut(s)	1227
EcoO65I	GGTNACC	1 cut(s)	1171
EcoRII	CCWGG	2 cut(s)	1202, 1429
EcoT14I	CCWWGG	3 cut(s)	504, 530, 1062
EcoT38I	GRGCYC	1 cut(s)	1229
ErhI	CCWWGG	3 cut(s)	504, 530, 1062
FaeI	CATG	7 cut(s)	57, 508, 854, 866, 1023, 1066, 1075
FalI	AAGNNNNNCTT	4 cut(s)	694, 726, 862, 894
FaqI	GGGAC	1 cut(s)	889
FatI	CATG	7 cut(s)	53, 504, 850, 862, 1019, 1062, 1071
Fnu4HI	GCNGC	1 cut(s)	221
FokI	GGATG	2 cut(s)	245, 1259
FriOI	GRGCYC	1 cut(s)	1229
FseI	GGCCGGCC	1 cut(s)	617
Fsp4HI	GCNGC	1 cut(s)	221
FspBI	CTAG	2 cut(s)	38, 1274
GluI	GCNGC	1 cut(s)	221
GsaI	CCCAGC	1 cut(s)	206
HaeIII	GGCC	5 cut(s)	5, 613, 617, 922, 1061
HapII	CCGG	6 cut(s)	6, 273, 375, 614, 1176, 1403
HgaI	GACGC	1 cut(s)	1346
Hin1II	CATG	7 cut(s)	57, 508, 854, 866, 1023, 1066, 1075
HinfI	GANTC	3 cut(s)	41, 278, 681
HpaII	CCGG	6 cut(s)	6, 273, 375, 614, 1176, 1403
HphI	GGTGA	1 cut(s)	1150
Hpy188I	TCNGA	4 cut(s)	190, 708, 963, 1128
Hpy188III	TCNNGA	4 cut(s)	173, 262, 1274, 1418
Hpy99I	CGWCG	2 cut(s)	1141, 1340
HpyAV	CCTTC	1 cut(s)	1265
HpyCH4III	ACNGT	8 cut(s)	70, 229, 417, 737, 774, 957, 1168, 1327
HpyCH4IV	ACGT	2 cut(s)	423, 1136
HpyCH4V	TGCA	5 cut(s)	258, 341, 626, 746, 946
HpyF10VI	GCNNNNNNNGC	3 cut(s)	623, 889, 928
HpyF3I	CTNAG	3 cut(s)	831, 1038, 1125
HpySE526I	ACGT	2 cut(s)	423, 1136
Hsp92II	CATG	7 cut(s)	57, 508, 854, 866, 1023, 1066, 1075
KpnI	GGTACC	1 cut(s)	773
KroI	GCCGGC	1 cut(s)	613
KroNI	GCCGGC	1 cut(s)	615
Kzo9I	GATC	2 cut(s)	180, 526
LguI	GCTCTTC	2 cut(s)	464, 1192
Lsp1109I	GCAGC	1 cut(s)	207
LweI	GCATC	6 cut(s)	267, 780, 831, 1077, 1237, 1294
MaeI	CTAG	2 cut(s)	38, 1274
MaeII	ACGT	2 cut(s)	423, 1136
MaeIII	GTNAC	5 cut(s)	590, 896, 1096, 1120, 1171
MalI	GATC	2 cut(s)	182, 528
MboI	GATC	2 cut(s)	180, 526
MfeI	CAATTG	1 cut(s)	974
MhlI	GDGCHC	2 cut(s)	411, 1229
MlsI	TGGCCA	1 cut(s)	1061
MluCI	AATT	7 cut(s)	515, 632, 729, 757, 926, 974, 1004
MluNI	TGGCCA	1 cut(s)	1061
MlyI	GAGTC	1 cut(s)	287
MmeI	TCCRAC	1 cut(s)	566
Mox20I	TGGCCA	1 cut(s)	1061
MroNI	GCCGGC	1 cut(s)	613
MroXI	GAANNNNTTC	1 cut(s)	355
MscI	TGGCCA	1 cut(s)	1061
MseI	TTAA	2 cut(s)	692, 1221
MslI	CAYNNNNRTG	3 cut(s)	52, 1127, 1302
Msp20I	TGGCCA	1 cut(s)	1061
MspA1I	CMGCKG	1 cut(s)	544
MspCI	CTTAAG	1 cut(s)	1220
MspI	CCGG	6 cut(s)	6, 273, 375, 614, 1176, 1403
MspR9I	CCNGG	2 cut(s)	1204, 1431
MunI	CAATTG	1 cut(s)	974
Mva1269I	GAATGC	1 cut(s)	980
MvaI	CCWGG	2 cut(s)	1204, 1431
MwoI	GCNNNNNNNGC	3 cut(s)	623, 889, 928
NaeI	GCCGGC	1 cut(s)	615
NcoI	CCATGG	2 cut(s)	504, 1062
NdeII	GATC	2 cut(s)	180, 526
NgoMIV	GCCGGC	1 cut(s)	613
NlaIII	CATG	7 cut(s)	57, 508, 854, 866, 1023, 1066, 1075
NlaIV	GGNNCC	2 cut(s)	379, 771
NmeAIII	GCCGAG	1 cut(s)	1208
NmuCI	GTSAC	3 cut(s)	590, 896, 1120
PciSI	GCTCTTC	2 cut(s)	464, 1192
PctI	GAATGC	1 cut(s)	980
PdiI	GCCGGC	1 cut(s)	615
PdmI	GAANNNNTTC	1 cut(s)	355
PfeI	GAWTC	2 cut(s)	41, 681
PkrI	GCNGC	1 cut(s)	222
PleI	GAGTC	1 cut(s)	286
PpsI	GAGTC	1 cut(s)	286
PshBI	ATTAAT	1 cut(s)	692
PsiI	TTATAA	1 cut(s)	756
Psp124BI	GAGCTC	1 cut(s)	1229
Psp6I	CCWGG	2 cut(s)	1202, 1429
PspEI	GGTNACC	1 cut(s)	1171
PspFI	CCCAGC	1 cut(s)	202
PspGI	CCWGG	2 cut(s)	1202, 1429
PspN4I	GGNNCC	2 cut(s)	379, 771
PspPI	GGNCC	3 cut(s)	372, 921, 1433
PstI	CTGCAG	1 cut(s)	748
PvuII	CAGCTG	1 cut(s)	544
RigI	GGCCGGCC	1 cut(s)	617
RsaI	GTAC	6 cut(s)	771, 1001, 1077, 1135, 1386, 1401
RsaNI	GTAC	6 cut(s)	770, 1000, 1076, 1134, 1385, 1400
RseI	CAYNNNNRTG	3 cut(s)	52, 1127, 1302
SacI	GAGCTC	1 cut(s)	1229
SapI	GCTCTTC	2 cut(s)	464, 1192
SaqAI	TTAA	2 cut(s)	692, 1221
SatI	GCNGC	1 cut(s)	221
Sau3AI	GATC	2 cut(s)	180, 526
Sau96I	GGNCC	3 cut(s)	372, 921, 1433
SchI	GAGTC	1 cut(s)	287
ScrFI	CCNGG	2 cut(s)	1204, 1431
SduI	GDGCHC	2 cut(s)	411, 1229
SfaNI	GCATC	6 cut(s)	267, 780, 831, 1077, 1237, 1294
SfcI	CTRYAG	1 cut(s)	744
SinI	GGWCC	2 cut(s)	372, 1433
SmiMI	CAYNNNNRTG	3 cut(s)	52, 1127, 1302
SmlI	CTYRAG	2 cut(s)	307, 1220
SmoI	CTYRAG	2 cut(s)	307, 1220
Sse9I	AATT	7 cut(s)	515, 632, 729, 757, 926, 974, 1004
SsiI	CCGC	1 cut(s)	1409
SspMI	CTAG	2 cut(s)	38, 1274
SstI	GAGCTC	1 cut(s)	1229
StyD4I	CCNGG	2 cut(s)	1202, 1429
StyI	CCWWGG	3 cut(s)	504, 530, 1062
TaaI	ACNGT	8 cut(s)	70, 229, 417, 737, 774, 957, 1168, 1327
TaiI	ACGT	2 cut(s)	426, 1139
TaqI	TCGA	6 cut(s)	105, 368, 673, 1139, 1335, 1417
TasI	AATT	7 cut(s)	515, 632, 729, 757, 926, 974, 1004
TatI	WGTACW	1 cut(s)	1384
TfiI	GAWTC	2 cut(s)	41, 681
Tru1I	TTAA	2 cut(s)	692, 1221
Tru9I	TTAA	2 cut(s)	692, 1221
TscAI	CASTG	5 cut(s)	420, 740, 901, 960, 1173
TseFI	GTSAC	3 cut(s)	590, 896, 1120
TseI	GCWGC	1 cut(s)	220
Tsp45I	GTSAC	3 cut(s)	590, 896, 1120
TspDTI	ATGAA	6 cut(s)	79, 107, 199, 348, 482, 673
TspGWI	ACGGA	2 cut(s)	596, 693
TspRI	CASTG	5 cut(s)	420, 740, 901, 960, 1173
Vha464I	CTTAAG	1 cut(s)	1220
VpaK11BI	GGWCC	2 cut(s)	372, 1433
VspI	ATTAAT	1 cut(s)	692
XapI	RAATTY	1 cut(s)	1004
XbaI	TCTAGA	1 cut(s)	1273
XcmI	CCANNNNNNNNNTGG	2 cut(s)	560, 1069
XmnI	GAANNNNTTC	1 cut(s)	355
XspI	CTAG	2 cut(s)	38, 1274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.