pycom10g24570

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011139
Physical Location & Seq
Forward (+)
26409817 .. 26411994
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGACTGGAGGGTCATTGGGGATTCGATCAGGGAGTTATGGTTCTTTGGATAAACAGCTACACAACAACATCAACAGCATCAACGGAGTGTTGCCAATCCAATCTGCGCGTAAGCCTTCGAAAATGCTCAAGGAGAAGGAGAGGTTGTTCCACTGGATCTGCAAGTTTGCCGGCCGGAAGAAGGTTGGAATGCTGCTGCTCTGCCTCATCTCCGCCGCGGTTTTTGTCTGGGTTTTGTACGTTGGAAAAGGTGAAGACGGACAAGATGTTGTCAATGTCGGAAACATTAGTGTTAACAACAGCATCACAAGTTTTTCTGAATCCTCGCGAAAACATGGACCTCAAAGTAACAACATGACTTTTTTGCGGGCGTTGTCTCCTCCTCCTCCTGCTGTTTTTCTGGGTTACACTCTTCCTCCTGGACATCCATGTAATACATTCACTTTGCCTCCCCCACCAGCAGATAAAAAAAGAACTGGACCACGACCTTGTCCAGTATGTTACCTTCCTGTGGAAGAAGCTATAGCCTTGATGCCCAAGGTCCCTTCATATTCTCCAGTTCTGAAGAATTTAACATACATTTATGAAGAAAATTTATCTAGAAAAACAGAATTTGGAGGCTCAGAGTTTGGTGGATATCCTACATTGAAGCAAAGGAGTGATTCGTATGATATAAGAGAGTCAATGACTGTGCACTGTGGATTTGTTAGAGGAAGTAAACCTGGCCGCAATACTGGATATGATCTGGATGAGATTGACCTAAATGATATGGAGCAGTGTAATGGAGTGGTTGTTGCATCAGCTATATTTGGAAATTTTGATGAGATAAACCAGCCAAGGAAGATTAGTGATTATTCCAAGGAGACCGTTTGCTTCTACATGTTTATAGATGATGTGACAGAAGCTTACTTGAGAACATCTGGGAAAATGGGCAGCGATAGGAAAGTTGGTATATGGAGAACTGTGGTTGTGCATAACCCTCCTTACACGGATGGAAGGCGCACTGGGAAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

339

Amino Acids

37.42

Weight (kDa)

9.06

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 191 - 337 4.6e-54 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccII CGCG 3 cut(s) 109, 218, 328
AciI CCGC 5 cut(s) 213, 216, 218, 367, 727
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 2 cut(s) 172, 724
AcsI RAATTY 4 cut(s) 568, 592, 611, 814
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 181, 511
AflIII ACRYGT 1 cut(s) 879
AgsI TTSAA 1 cut(s) 649
AjnI CCWGG 2 cut(s) 418, 721
AloI GAACNNNNNNTCC 4 cut(s) 25, 57, 131, 163
AluBI AGCT 4 cut(s) 58, 521, 803, 905
AluI AGCT 4 cut(s) 58, 521, 803, 905
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 2 cut(s) 381, 857
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 164
AoxI GGCC 2 cut(s) 172, 724
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 3 cut(s) 193, 196, 933
ApoI RAATTY 4 cut(s) 568, 592, 611, 814
AspLEI GCGC 2 cut(s) 109, 1002
AspS9I GGNCC 3 cut(s) 338, 479, 541
AsuHPI GGTGA 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 3 cut(s) 338, 479, 541
BaeGI GKGCMC 1 cut(s) 696
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 180, 183, 945
BccI CCATC 1 cut(s) 986
BciT130I CCWGG 2 cut(s) 420, 723
BcoDI GTCTC 2 cut(s) 381, 857
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 5 cut(s) 194, 197, 216, 727, 934
BlsI GCNGC 5 cut(s) 195, 198, 217, 728, 935
Bme1390I CCNGG 2 cut(s) 420, 723
Bme18I GGWCC 3 cut(s) 338, 479, 541
BmgT120I GGNCC 3 cut(s) 338, 479, 541
BmiI GGNNCC 1 cut(s) 543
BmrFI CCNGG 2 cut(s) 420, 723
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 4 cut(s) 87, 312, 522, 806
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 2 cut(s) 27, 540
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 113, 931
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 4 cut(s) 216, 537, 836, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 511
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 7 cut(s) 10, 158, 481, 494, 557, 739, 1009
BseBI CCWGG 2 cut(s) 420, 723
BseDI CCNNGG 4 cut(s) 216, 537, 836, 858
BseGI GGATG 3 cut(s) 424, 754, 997
BseLI CCNNNNNNNGG 2 cut(s) 181, 511
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 7 cut(s) 10, 158, 481, 494, 557, 739, 1009
BseRI GAGGAG 3 cut(s) 369, 372, 375
BseSI GKGCMC 1 cut(s) 696
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 180, 183, 945
Bsh1236I CGCG 3 cut(s) 109, 218, 328
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 2 cut(s) 174, 726
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 696
BsiSI CCGG 2 cut(s) 171, 175
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 2 cut(s) 181, 511
BsmAI GTCTC 2 cut(s) 381, 857
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 2 cut(s) 174, 726
Bso31I GGTCTC 1 cut(s) 857
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 696
Bsp143I GATC 3 cut(s) 26, 156, 742
Bsp68I TCGCGA 1 cut(s) 328
BspACI CCGC 5 cut(s) 213, 216, 218, 367, 727
BspANI GGCC 2 cut(s) 174, 726
BspCNI CTCAG 1 cut(s) 635
BspFNI CGCG 3 cut(s) 109, 218, 328
BspLI GGNNCC 1 cut(s) 543
BspPI GGATC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 857
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 7 cut(s) 10, 158, 481, 494, 557, 739, 1009
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 216, 537, 836, 858
BssMI GATC 3 cut(s) 26, 156, 742
BssT1I CCWWGG 3 cut(s) 537, 836, 858
Bst2UI CCWGG 2 cut(s) 420, 723
Bst4CI ACNGT 4 cut(s) 691, 698, 868, 964
Bst6I CTCTTC 1 cut(s) 417
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 172, 369
BstDEI CTNAG 1 cut(s) 622
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 3 cut(s) 424, 754, 997
BstFNI CGCG 3 cut(s) 109, 218, 328
BstHHI GCGC 2 cut(s) 109, 1002
BstKTI GATC 3 cut(s) 29, 159, 745
BstMAI GTCTC 2 cut(s) 381, 857
BstMBI GATC 3 cut(s) 26, 156, 742
BstMCI CGRYCG 1 cut(s) 175
BstNI CCWGG 2 cut(s) 420, 723
BstNSI RCATGY 1 cut(s) 883
BstSCI CCNGG 2 cut(s) 418, 721
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 3 cut(s) 109, 218, 328
BstV1I GCAGC 3 cut(s) 180, 183, 945
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BstZI CGGCCG 1 cut(s) 172
BsuRI GGCC 2 cut(s) 174, 726
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 3 cut(s) 424, 754, 997
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 4 cut(s) 151, 694, 782, 1002
BtuMI TCGCGA 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 172, 369
CfoI GCGC 2 cut(s) 109, 1002
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 3 cut(s) 338, 479, 541
Cfr42I CCGCGG 1 cut(s) 219
Csp6I GTAC 1 cut(s) 238
CviAII CATG 4 cut(s) 335, 355, 429, 880
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 622
DpnI GATC 3 cut(s) 28, 158, 744
DpnII GATC 3 cut(s) 26, 156, 742
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 172, 724
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 1 cut(s) 202
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 3 cut(s) 537, 836, 858
Eco31I GGTCTC 1 cut(s) 857
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 3 cut(s) 338, 479, 541
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 418, 721
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 3 cut(s) 537, 836, 858
ErhI CCWWGG 3 cut(s) 537, 836, 858
FaeI CATG 4 cut(s) 338, 358, 432, 883
FaqI GGGAC 1 cut(s) 527
FatI CATG 4 cut(s) 334, 354, 428, 879
FauI CCCGC 1 cut(s) 360
Fnu4HI GCNGC 5 cut(s) 194, 197, 216, 727, 934
FokI GGATG 3 cut(s) 411, 761, 1004
Fsp4HI GCNGC 5 cut(s) 194, 197, 216, 727, 934
FspBI CTAG 1 cut(s) 600
GlaI GCGC 2 cut(s) 108, 1001
GluI GCNGC 5 cut(s) 194, 197, 216, 727, 934
GsuI CTGGAG 2 cut(s) 27, 540
HaeIII GGCC 2 cut(s) 174, 726
HapII CCGG 2 cut(s) 171, 175
HhaI GCGC 2 cut(s) 109, 1002
Hin1II CATG 4 cut(s) 338, 358, 432, 883
Hin6I GCGC 2 cut(s) 107, 1000
HinP1I GCGC 2 cut(s) 107, 1000
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 4 cut(s) 22, 320, 662, 680
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 2 cut(s) 171, 175
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 3 cut(s) 295, 694, 719
Hpy188I TCNGA 4 cut(s) 281, 319, 564, 625
Hpy188III TCNNGA 3 cut(s) 327, 600, 746
Hpy8I GTNNAC 3 cut(s) 295, 694, 719
HpyAV CCTTC 6 cut(s) 126, 130, 175, 515, 555, 990
HpyCH4III ACNGT 4 cut(s) 691, 698, 868, 964
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 4 cut(s) 162, 694, 797, 973
HpyF3I CTNAG 1 cut(s) 622
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 4 cut(s) 338, 358, 432, 883
HspAI GCGC 2 cut(s) 107, 1000
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspAI GTTAAC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 219
Kzo9I GATC 3 cut(s) 26, 156, 742
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 3 cut(s) 180, 183, 945
LweI GCATC 4 cut(s) 87, 312, 522, 806
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 4 cut(s) 347, 404, 500, 895
MalI GATC 3 cut(s) 28, 158, 744
MboI GATC 3 cut(s) 26, 156, 742
MboII GAAGA 7 cut(s) 190, 266, 404, 527, 577, 599, 853
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 4 cut(s) 568, 592, 611, 814
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 3 cut(s) 166, 223, 259
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 2 cut(s) 294, 572
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 2 cut(s) 171, 175
MspR9I CCNGG 2 cut(s) 420, 723
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 2 cut(s) 420, 723
MvnI CGCG 3 cut(s) 109, 218, 328
NaeI GCCGGC 1 cut(s) 172
NdeII GATC 3 cut(s) 26, 156, 742
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 4 cut(s) 338, 358, 432, 883
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 895
NruI TCGCGA 1 cut(s) 328
NspI RCATGY 1 cut(s) 883
NspV TTCGAA 1 cut(s) 119
PciI ACATGT 1 cut(s) 879
PctI GAATGC 1 cut(s) 195
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 22, 320, 662
PflFI GACNNNGTC 1 cut(s) 489
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 5 cut(s) 195, 198, 217, 728, 935
PleI GAGTC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 541
PscI ACATGT 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 2 cut(s) 418, 721
PspGI CCWGG 2 cut(s) 418, 721
PspN4I GGNNCC 1 cut(s) 543
PspPI GGNCC 3 cut(s) 338, 479, 541
PspPPI RGGWCCY 1 cut(s) 541
PsuI RGATCY 1 cut(s) 156
PsyI GACNNNGTC 1 cut(s) 489
RruI TCGCGA 1 cut(s) 328
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SacII CCGCGG 1 cut(s) 219
SaqAI TTAA 2 cut(s) 294, 572
SatI GCNGC 5 cut(s) 194, 197, 216, 727, 934
Sau3AI GATC 3 cut(s) 26, 156, 742
Sau96I GGNCC 3 cut(s) 338, 479, 541
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 2 cut(s) 420, 723
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 4 cut(s) 87, 312, 522, 806
SfcI CTRYAG 1 cut(s) 522
Sfr303I CCGCGG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 219
SinI GGWCC 3 cut(s) 338, 479, 541
SmlI CTYRAG 2 cut(s) 128, 910
SmoI CTYRAG 2 cut(s) 128, 910
Sse9I AATT 4 cut(s) 568, 592, 611, 814
SsiI CCGC 5 cut(s) 213, 216, 218, 367, 727
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 2 cut(s) 418, 721
StyI CCWWGG 3 cut(s) 537, 836, 858
TaaI ACNGT 4 cut(s) 691, 698, 868, 964
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 25, 119
TasI AATT 4 cut(s) 568, 592, 611, 814
TauI GCSGC 2 cut(s) 218, 729
TfiI GAWTC 3 cut(s) 22, 320, 662
Tru1I TTAA 2 cut(s) 294, 572
Tru9I TTAA 2 cut(s) 294, 572
TscAI CASTG 4 cut(s) 158, 701, 782, 1009
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 3 cut(s) 193, 196, 933
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 2 cut(s) 537, 600
TspGWI ACGGA 3 cut(s) 99, 273, 1004
TspRI CASTG 4 cut(s) 158, 701, 782, 1009
Tth111I GACNNNGTC 1 cut(s) 489
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 3 cut(s) 338, 479, 541
XapI RAATTY 4 cut(s) 568, 592, 611, 814
XbaI TCTAGA 1 cut(s) 599
XceI RCATGY 1 cut(s) 883
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.