pycom10g25420

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012928
Physical Location & Seq
Reverse (-)
27076266 .. 27078389
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 942 bp
ATGGAGAAGAAGTACAGATACTTTGGGAGATTTTCCTTCCATTGGTTTGTTTTGTTGGCTTTTGTTTGCAGTTTAGTTCCAAGAGCTCAAGGATGGAGCAAAGAGGGTCATATTATGACGTGCCGGATTGCGCAGGCACTTCTAAAGCCCGAGGCCGCAGAAGCTGTTCGAAATTTACTACCTCACAACGTCGATGGTGATTTATCAGCTCTCTGCGTATGGCCGGATCAGGTCAGGCATTGGTACAGATACAGATGGACTAGCCCGCTTCACTTCATCGATACACCGGATAATGCTTGCACCTTTGACTACTCAAGGGATTGTCATGATCAACATGGCGTGAAGAACATGTGTGTTGCTGGTGCCGTCCAAAACTTTACCTCTCAGCTTTCACACTACACAGAAGGAACATCTGACCGTCGATATAATATGACTGAGGCCTTGCTTTTCTTGTCACACTTTATGGGAGATATCCATCAGCCAATGCATGTCGGGTTCACGACAGATGAGGGAGGAAACACGATAGACTTGCGTTGGTACAAGCACAAATCCAATCTTCACCATGTATGGGATAGGGAGATTATACAACAAGCTATGAAGGACTACTATGACAAAGACATGGAACTTCTCCTACAAGACATACAGGGGAACATCACAGATGGAATCTGGTCTGATGATGTTACATCGTGGAAACATTGCGATGATCAACGTTCATGTATAAACAAGTATGCTACGGAGAGTGTAAACATAGCTTGCAAGTGGGGTTACAAGGACGTTGGGGTGGGTGATACTCTTACAGAGGATTACTTCCTTCCTCGGCTACCTGTGGTGGAGAAGAGAATTGCTCAAGGCGGGGTTCGCTTGGCGGCCATTCTCAATCGGGTCTTCTCTTCCGATCAAGAGACAGAAGTTGCTGACGAAGCATCACACTCACCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

314

Amino Acids

36.18

Weight (kDa)

5.86

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 32 - 296 9e-88 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 362
AciI CCGC 4 cut(s) 156, 266, 852, 866
AclI AACGTT 1 cut(s) 709
AclWI GGATC 1 cut(s) 234
AcoI YGGCCR 2 cut(s) 221, 867
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 3 cut(s) 14, 245, 539
AfiI CCNNNNNNNGG 2 cut(s) 42, 568
AflIII ACRYGT 1 cut(s) 348
AjiI CACGTC 1 cut(s) 120
AluBI AGCT 6 cut(s) 86, 164, 209, 388, 593, 752
AluI AGCT 6 cut(s) 86, 164, 209, 388, 593, 752
Alw21I GWGCWC 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 896
AlwI GGATC 1 cut(s) 234
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 4 cut(s) 153, 221, 438, 867
ApoI RAATTY 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 165
AspLEI GCGC 1 cut(s) 133
AsuHPI GGTGA 4 cut(s) 209, 551, 797, 924
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 149
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 88
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 88
BccI CCATC 5 cut(s) 87, 188, 249, 483, 653
BceAI ACGGC 1 cut(s) 350
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BclI TGATCA 2 cut(s) 328, 703
BcoDI GTCTC 1 cut(s) 896
BfaI CTAG 1 cut(s) 261
BisI GCNGC 2 cut(s) 156, 867
BlsI GCNGC 2 cut(s) 157, 868
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 364
BmsI GCATC 1 cut(s) 932
BpiI GAAGAC 1 cut(s) 877
BplI GAGNNNNNCTC 2 cut(s) 829, 861
Bpu14I TTCGAA 1 cut(s) 169
BpuEI CTTGAG 3 cut(s) 72, 298, 831
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 150, 815
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 568
Bse3DI GCAATG 1 cut(s) 694
Bse8I GATNNNNATC 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 150, 815
BseGI GGATG 1 cut(s) 98
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 42, 568
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 2 cut(s) 398, 426
BshFI GGCC 4 cut(s) 155, 223, 440, 869
BshNI GGYRCC 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 124, 224, 287
BslI CCNNNNNNNGG 2 cut(s) 42, 568
BsmAI GTCTC 1 cut(s) 896
BsnI GGCC 4 cut(s) 155, 223, 440, 869
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 88
Bsp143I GATC 4 cut(s) 226, 328, 703, 895
BspACI CCGC 4 cut(s) 156, 266, 852, 866
BspANI GGCC 4 cut(s) 155, 223, 440, 869
BspCNI CTCAG 2 cut(s) 397, 427
BspDI ATCGAT 1 cut(s) 279
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 694
BssECI CCNNGG 2 cut(s) 150, 815
BssMI GATC 4 cut(s) 226, 328, 703, 895
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 2 cut(s) 830, 895
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 4 cut(s) 135, 266, 298, 754
BstDEI CTNAG 2 cut(s) 384, 435
BstF5I GGATG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 4 cut(s) 229, 331, 706, 898
BstMAI GTCTC 1 cut(s) 896
BstMBI GATC 4 cut(s) 226, 328, 703, 895
BstMWI GCNNNNNNNGC 3 cut(s) 161, 858, 920
BstNSI RCATGY 2 cut(s) 352, 491
BstV2I GAAGAC 1 cut(s) 877
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 4 cut(s) 155, 223, 440, 869
BsuTUI ATCGAT 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 714
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 1 cut(s) 98
Cac8I GCNNGC 4 cut(s) 135, 266, 298, 754
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 3 cut(s) 13, 244, 538
CviAII CATG 7 cut(s) 326, 335, 349, 488, 563, 619, 714
CviQI GTAC 3 cut(s) 13, 244, 538
DdeI CTNAG 2 cut(s) 384, 435
DpnI GATC 4 cut(s) 228, 330, 705, 897
DpnII GATC 4 cut(s) 226, 328, 703, 895
EaeI YGGCCR 2 cut(s) 221, 867
Eam1104I CTCTTC 2 cut(s) 830, 895
EarI CTCTTC 2 cut(s) 830, 895
Ecl136II GAGCTC 1 cut(s) 86
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 472
Eco53kI GAGCTC 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 86
EcoRV GATATC 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 88
FaeI CATG 7 cut(s) 329, 338, 352, 491, 566, 622, 717
FatI CATG 7 cut(s) 325, 334, 348, 487, 562, 618, 713
FauI CCCGC 2 cut(s) 273, 845
FbaI TGATCA 2 cut(s) 328, 703
Fnu4HI GCNGC 2 cut(s) 156, 867
FokI GGATG 1 cut(s) 105
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 2 cut(s) 156, 867
FspBI CTAG 1 cut(s) 261
FspI TGCGCA 1 cut(s) 132
GlaI GCGC 1 cut(s) 132
GluI GCNGC 2 cut(s) 156, 867
HaeIII GGCC 4 cut(s) 155, 223, 440, 869
HapII CCGG 3 cut(s) 124, 224, 287
HhaI GCGC 1 cut(s) 133
Hin1II CATG 7 cut(s) 329, 338, 352, 491, 566, 622, 717
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 1 cut(s) 663
HpaII CCGG 3 cut(s) 124, 224, 287
HphI GGTGA 4 cut(s) 209, 551, 797, 924
Hpy166II GTNNAC 2 cut(s) 498, 745
Hpy188I TCNGA 3 cut(s) 415, 673, 895
Hpy188III TCNNGA 3 cut(s) 326, 499, 899
Hpy8I GTNNAC 2 cut(s) 498, 745
Hpy99I CGWCG 2 cut(s) 194, 423
HpyAV CCTTC 4 cut(s) 46, 398, 592, 821
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 4 cut(s) 119, 189, 709, 774
HpyCH4V TGCA 4 cut(s) 69, 300, 487, 756
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 858, 920
HpyF3I CTNAG 2 cut(s) 384, 435
HpySE526I ACGT 4 cut(s) 119, 189, 709, 774
Hsp92II CATG 7 cut(s) 329, 338, 352, 491, 566, 622, 717
HspAI GCGC 1 cut(s) 131
Ksp22I TGATCA 2 cut(s) 328, 703
Kzo9I GATC 4 cut(s) 226, 328, 703, 895
LmnI GCTCC 1 cut(s) 96
LweI GCATC 1 cut(s) 932
MaeI CTAG 1 cut(s) 261
MaeII ACGT 4 cut(s) 119, 189, 709, 774
MaeIII GTNAC 3 cut(s) 453, 679, 764
MalI GATC 4 cut(s) 228, 330, 705, 897
MboI GATC 4 cut(s) 226, 328, 703, 895
MboII GAAGA 6 cut(s) 19, 355, 548, 847, 877, 882
MhlI GDGCHC 1 cut(s) 88
MluCI AATT 2 cut(s) 172, 840
MnlI CCTC 9 cut(s) 97, 145, 192, 391, 430, 502, 506, 793, 825
Mph1103I ATGCAT 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 699
MspI CCGG 3 cut(s) 124, 224, 287
MwoI GCNNNNNNNGC 3 cut(s) 161, 858, 920
NdeII GATC 4 cut(s) 226, 328, 703, 895
NlaIII CATG 7 cut(s) 329, 338, 352, 491, 566, 622, 717
NlaIV GGNNCC 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 796
NmuCI GTSAC 1 cut(s) 453
NsbI TGCGCA 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 2 cut(s) 352, 491
NspV TTCGAA 1 cut(s) 169
PagI TCATGA 1 cut(s) 325
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 663
PkrI GCNGC 2 cut(s) 157, 868
PscI ACATGT 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 88
Psp1406I AACGTT 1 cut(s) 709
PspN4I GGNNCC 1 cut(s) 364
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstNI CAGNNNCTG 1 cut(s) 164
RsaI GTAC 3 cut(s) 14, 245, 539
RsaNI GTAC 3 cut(s) 13, 244, 538
RseI CAYNNNNRTG 1 cut(s) 699
SacI GAGCTC 1 cut(s) 88
SatI GCNGC 2 cut(s) 156, 867
Sau3AI GATC 4 cut(s) 226, 328, 703, 895
SduI GDGCHC 1 cut(s) 88
SfaNI GCATC 1 cut(s) 932
SfuI TTCGAA 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 3 cut(s) 87, 313, 846
SmoI CTYRAG 3 cut(s) 87, 313, 846
Sse9I AATT 2 cut(s) 172, 840
SseBI AGGCCT 1 cut(s) 440
SsiI CCGC 4 cut(s) 156, 266, 852, 866
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 88
StuI AGGCCT 1 cut(s) 440
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 4 cut(s) 122, 192, 712, 777
TaqI TCGA 4 cut(s) 169, 192, 279, 421
TasI AATT 2 cut(s) 172, 840
TatI WGTACW 1 cut(s) 12
TauI GCSGC 2 cut(s) 158, 869
TfiI GAWTC 1 cut(s) 663
TseFI GTSAC 1 cut(s) 453
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 3 cut(s) 265, 611, 702
TspGWI ACGGA 1 cut(s) 749
XapI RAATTY 1 cut(s) 172
XceI RCATGY 2 cut(s) 352, 491
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 1 cut(s) 261
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.