pycom10g26090

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010974
Physical Location & Seq
Forward (+)
27504659 .. 27505636
978 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGTCTCTGACACCAGTATCTTTGCTCACTCTCTGTATCCTTCTCCTTCTCCTTCTTCCTCCCACCGCCACCTTAACCCTCCACAACCTCCTCCAAAGCCAAGGCCTTCCTGCCGGGCTCTTCCCGGACAATGTGAAGTCCTACAATCTCGATCAGATGGGCCACCTGGAGGTGCATCTGGACCGTTCTTGCATGACCAAGTTCGAGACAAGAGTCTACTTTGAGAGCATAGTCAGAGCTAATCTCAGCTATGGCGTGCTCAGAGGCTTGGAGGGTTTGACTCAGGAGGAGCTTTTTCTTTGGCTGCCGGTGAAGGGAATCTCAGTGAGCGATCCGTCTTCTGGGTTGATTTTGTTTGACATTGGTGTTGCCCATAAGCAGCTTTCTCTCTCTCTATTTGAAGACCCCCCTGTCTGCAAGCCTCAATCTCAAGGCATTTCATTGAAGAGTGAAGGAGGAAGGAAGATTGGATTTCAGGCCCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

163

Amino Acids

17.82

Weight (kDa)

6.4

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 134 5.5e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61667 AT1G61667
fragaria_vesca FvH4_3g03180
malus_domestica MD05G1334600.v1.1 MD10G1309500.v1.1
prunus_persica Prupe.4G030700_v2.0.a1
pyrus_communis pycom05g30610 pycom10g26090
rosa_chinensis RchiOBHm_Chr5g0004811
rosa_laevigata RLG00000031262
rosa_multiflora Rmu_co7972276.1_g000001 Rmu_sc0008308.1_g000015 Rmu_sc0010871.1_g000007
rosa_roxburghii Rroxscaffold_1G00070800
rosa_rugosa Rorug04G0414000
rosa_samantha Rh5AG041800 Rh5BG041500 Rh5CG045400 Rh5DG041900
rosa_wichuraiana Rw5G004050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 216
AciI CCGC 1 cut(s) 66
AclWI GGATC 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 169, 314, 341
AgsI TTSAA 2 cut(s) 401, 445
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 4 cut(s) 239, 249, 292, 382
AluI AGCT 4 cut(s) 239, 249, 292, 382
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 9, 200
AlwI GGATC 1 cut(s) 326
AoxI GGCC 3 cut(s) 103, 160, 477
ApeKI GCWGC 2 cut(s) 304, 379
AspS9I GGNCC 3 cut(s) 160, 181, 478
AsuC2I CCSGG 2 cut(s) 115, 125
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 181
BaeI ACNNNNGTAYC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 2 cut(s) 330, 408
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 291, 391
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 47
BcnI CCSGG 2 cut(s) 115, 125
BcoDI GTCTC 2 cut(s) 9, 200
BfuI GTATCC 1 cut(s) 47
BisI GCNGC 2 cut(s) 305, 380
BlsI GCNGC 2 cut(s) 306, 381
Bme1390I CCNGG 3 cut(s) 115, 125, 167
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 3 cut(s) 160, 181, 478
BmrFI CCNGG 3 cut(s) 115, 125, 167
BmsI GCATC 1 cut(s) 184
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 2 cut(s) 330, 408
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 2 cut(s) 115, 125
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 314, 341
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 169, 314, 341
BseMII CTCAG 4 cut(s) 259, 274, 296, 336
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 2 cut(s) 80, 302
BseXI GCAGC 2 cut(s) 291, 391
BshFI GGCC 3 cut(s) 105, 162, 479
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 114, 125, 308
BslI CCNNNNNNNGG 3 cut(s) 169, 314, 341
BsmAI GTCTC 2 cut(s) 9, 200
BsnI GGCC 3 cut(s) 105, 162, 479
Bsp1286I GDGCHC 2 cut(s) 120, 261
Bsp143I GATC 2 cut(s) 151, 331
BspACI CCGC 1 cut(s) 66
BspANI GGCC 3 cut(s) 105, 162, 479
BspCNI CTCAG 4 cut(s) 258, 273, 295, 335
BspPI GGATC 1 cut(s) 326
BspQI GCTCTTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 151, 331
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 2 cut(s) 125, 440
BstC8I GCNNGC 2 cut(s) 257, 419
BstDEI CTNAG 4 cut(s) 245, 260, 282, 322
BstKTI GATC 2 cut(s) 154, 334
BstMAI GTCTC 2 cut(s) 9, 200
BstMBI GATC 2 cut(s) 151, 331
BstNI CCWGG 1 cut(s) 167
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 3 cut(s) 113, 123, 165
BstV1I GCAGC 2 cut(s) 291, 391
BstV2I GAAGAC 2 cut(s) 330, 408
BsuI GTATCC 1 cut(s) 47
BsuRI GGCC 3 cut(s) 105, 162, 479
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 257, 419
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 3 cut(s) 160, 181, 478
CviAII CATG 1 cut(s) 193
DdeI CTNAG 4 cut(s) 245, 260, 282, 322
DpnI GATC 2 cut(s) 153, 333
DpnII GATC 2 cut(s) 151, 331
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 2 cut(s) 125, 440
EarI CTCTTC 2 cut(s) 125, 440
Eco130I CCWWGG 1 cut(s) 100
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 196
FaiI YATR 4 cut(s) 194, 230, 252, 375
FatI CATG 1 cut(s) 192
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 305, 380
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 305, 380
GluI GCNGC 2 cut(s) 305, 380
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 3 cut(s) 105, 162, 479
HapII CCGG 3 cut(s) 114, 125, 308
Hin1II CATG 1 cut(s) 196
HinfI GANTC 3 cut(s) 213, 280, 318
HpaII CCGG 3 cut(s) 114, 125, 308
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 4 cut(s) 9, 156, 236, 263
Hpy188III TCNNGA 4 cut(s) 149, 179, 205, 284
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 7 cut(s) 50, 56, 62, 116, 307, 446, 453
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4V TGCA 3 cut(s) 175, 192, 417
HpyF3I CTNAG 4 cut(s) 245, 260, 282, 322
Hsp92II CATG 1 cut(s) 196
Kzo9I GATC 2 cut(s) 151, 331
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 291, 391
LweI GCATC 1 cut(s) 184
MalI GATC 2 cut(s) 153, 333
MboI GATC 2 cut(s) 151, 331
MboII GAAGA 6 cut(s) 47, 112, 330, 413, 457, 475
MhlI GDGCHC 2 cut(s) 120, 261
MlyI GAGTC 2 cut(s) 222, 274
MseI TTAA 1 cut(s) 74
MspI CCGG 3 cut(s) 114, 125, 308
MspR9I CCNGG 3 cut(s) 115, 125, 167
MvaI CCWGG 1 cut(s) 167
NciI CCSGG 2 cut(s) 115, 125
NdeII GATC 2 cut(s) 151, 331
NlaIII CATG 1 cut(s) 196
PceI AGGCCT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 318
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 2 cut(s) 306, 381
PleI GAGTC 2 cut(s) 221, 274
PpsI GAGTC 2 cut(s) 221, 274
PshAI GACNNNNGTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspPI GGNCC 3 cut(s) 160, 181, 478
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 2 cut(s) 305, 380
Sau3AI GATC 2 cut(s) 151, 331
Sau96I GGNCC 3 cut(s) 160, 181, 478
SchI GAGTC 2 cut(s) 222, 274
ScrFI CCNGG 3 cut(s) 115, 125, 167
SduI GDGCHC 2 cut(s) 120, 261
SetI ASST 8 cut(s) 74, 90, 168, 174, 241, 251, 294, 384
SfaNI GCATC 1 cut(s) 184
SinI GGWCC 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 1 cut(s) 66
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 113, 123, 165
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 1 cut(s) 185
TaqI TCGA 2 cut(s) 150, 204
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 1 cut(s) 330
TseI GCWGC 2 cut(s) 304, 379
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.