pycom10g29110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017258
Physical Location & Seq
Forward (+)
29506084 .. 29506698
615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGAGCGTGACAGCAAAAAGAGAGTAAGAGAAGGAGATGAAGACCACAACGATTCTTCTTCTTCTTCTTCTTGTTATTCTTTTTCAGAGAAGCATGCGAAGACAAATTTAGTAGATCCTAACTGTGGTATTTTGGACTTCAAAAACGACCTTGAATACAACAGCAGCGGCAGCACTAGTGGTAATAGTAGCAGTTATAAGACATCAGATTCGGATTCCAACTTGGCACTAGGAGTATTTGATTTTCCGTGGCTGAAAGATGGTGTAATATCCAAACCAGAGGACTTGAATTTTGAAGACGCATTTTCATCACCACTGGCAGTACTGGATGTCGACACATCCACCTTGACCACCAGTAGTACTACTCCTGGACATGGTAATTTTTCATCACCACTGGCAGCACTGGATGATCAATCTTCTTTCTCCGGGCAGTGCTTCTATCATGATCAGACTGACCATCCTGATGCACTACTCCTGGACTTTCCAAAGGACAAGCTTGAGATAGATTTGTGGCGACCTCGAGATCTTAATCAAGATAATGGGTCGAAGACTGAGGCCGGTGAAGATTGCATTTGGAGTTCCCTGCTCAAGCAGCCTCTTCAACAACCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.47

Weight (kDa)

4.36

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00131
malus_domestica MD10G1341800.v1.1
prunus_persica Prupe.4G001400_v2.0.a1
pyrus_communis pycom10g29110
rosa_chinensis RchiOBHm_Chr5g0000161
rosa_laevigata RLG00000030846
rosa_roxburghii Rroxscaffold_1G00075930
rosa_rugosa Rorug04G0382000
rosa_samantha Rh5AG001100 Rh5BG002500 Rh5CG001300 Rh5DG001200
rosa_wichuraiana Rw5G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 198
AccI GTMKAC 1 cut(s) 333
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 106, 289
AfaI GTAC 2 cut(s) 324, 361
AfiI CCNNNNNNNGG 2 cut(s) 125, 374
AgsI TTSAA 5 cut(s) 142, 155, 289, 296, 602
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 2 cut(s) 367, 474
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 519
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 4 cut(s) 165, 171, 398, 592
ApoI RAATTY 2 cut(s) 106, 289
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 275, 307
AsuC2I CCSGG 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 303, 381, 572
AvaI CYCGRG 1 cut(s) 519
BbsI GAAGAC 4 cut(s) 48, 107, 303, 554
BbvI GCAGC 4 cut(s) 177, 183, 410, 604
BccI CCATC 2 cut(s) 254, 465
BciT130I CCWGG 2 cut(s) 369, 476
BclI TGATCA 2 cut(s) 409, 445
BcnI CCSGG 1 cut(s) 427
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 2 cut(s) 177, 230
BglII AGATCT 1 cut(s) 523
BisI GCNGC 5 cut(s) 166, 169, 172, 399, 593
BlsI GCNGC 5 cut(s) 167, 170, 173, 400, 594
BmcAI AGTACT 2 cut(s) 324, 361
Bme1390I CCNGG 3 cut(s) 369, 427, 476
BmeT110I CYCGRG 1 cut(s) 519
BmrFI CCNGG 3 cut(s) 369, 427, 476
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 4 cut(s) 48, 107, 303, 554
BpuEI CTTGAG 2 cut(s) 518, 572
BpuMI CCSGG 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 248
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 374
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 5 cut(s) 321, 330, 354, 399, 408
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 2 cut(s) 369, 476
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 4 cut(s) 334, 338, 412, 457
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 2 cut(s) 125, 374
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 5 cut(s) 321, 330, 354, 399, 408
BseXI GCAGC 4 cut(s) 177, 183, 410, 604
BshFI GGCC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 2 cut(s) 426, 558
BslI CCNNNNNNNGG 2 cut(s) 125, 374
BsnI GGCC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 519
Bsp143I GATC 4 cut(s) 115, 409, 445, 523
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 1 cut(s) 544
BspHI TCATGA 1 cut(s) 442
BspPI GGATC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 5 cut(s) 321, 330, 354, 399, 408
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 4 cut(s) 115, 409, 445, 523
Bst2UI CCWGG 2 cut(s) 369, 476
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 4 cut(s) 334, 338, 412, 457
BstKTI GATC 4 cut(s) 118, 412, 448, 526
BstMBI GATC 4 cut(s) 115, 409, 445, 523
BstMWI GCNNNNNNNGC 2 cut(s) 171, 592
BstNI CCWGG 2 cut(s) 369, 476
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 367, 425, 474
BstV1I GCAGC 4 cut(s) 177, 183, 410, 604
BstV2I GAAGAC 4 cut(s) 48, 107, 303, 554
BstX2I RGATCY 2 cut(s) 115, 523
BstYI RGATCY 2 cut(s) 115, 523
BsuRI GGCC 1 cut(s) 557
BtgI CCRYGG 1 cut(s) 248
BtsCI GGATG 4 cut(s) 334, 338, 412, 457
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 4 cut(s) 314, 392, 401, 437
Cac8I GCNNGC 1 cut(s) 96
CciI TCATGA 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 557
CseI GACGC 1 cut(s) 308
Csp6I GTAC 2 cut(s) 323, 360
CviAII CATG 3 cut(s) 95, 374, 443
CviJI RGCY 4 cut(s) 253, 496, 557, 595
CviKI_1 RGCY 4 cut(s) 253, 496, 557, 595
CviQI GTAC 2 cut(s) 323, 360
DdeI CTNAG 1 cut(s) 552
DpnI GATC 4 cut(s) 117, 411, 447, 525
DpnII GATC 4 cut(s) 115, 409, 445, 523
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 367, 474
FaeI CATG 3 cut(s) 98, 377, 446
FaiI YATR 4 cut(s) 96, 198, 375, 444
FatI CATG 3 cut(s) 94, 373, 442
FbaI TGATCA 2 cut(s) 409, 445
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 166, 169, 172, 399, 593
FokI GGATG 4 cut(s) 325, 341, 419, 444
Fsp4HI GCNGC 5 cut(s) 166, 169, 172, 399, 593
FspBI CTAG 2 cut(s) 177, 230
GluI GCNGC 5 cut(s) 166, 169, 172, 399, 593
HaeIII GGCC 1 cut(s) 557
HapII CCGG 2 cut(s) 426, 558
HgaI GACGC 1 cut(s) 308
Hin1II CATG 3 cut(s) 98, 377, 446
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 3 cut(s) 53, 209, 215
HpaII CCGG 2 cut(s) 426, 558
HphI GGTGA 3 cut(s) 303, 381, 572
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 88, 208, 214, 450
Hpy188III TCNNGA 4 cut(s) 443, 461, 521, 533
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 467, 570
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 592
HpyF3I CTNAG 1 cut(s) 552
Hsp92II CATG 3 cut(s) 98, 377, 446
Ksp22I TGATCA 2 cut(s) 409, 445
Kzo9I GATC 4 cut(s) 115, 409, 445, 523
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 4 cut(s) 177, 183, 410, 604
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 177, 230
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 117, 411, 447, 525
MboI GATC 4 cut(s) 115, 409, 445, 523
MflI RGATCY 2 cut(s) 115, 523
MluCI AATT 3 cut(s) 106, 289, 379
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 4 cut(s) 274, 528, 547, 606
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 2 cut(s) 426, 558
MspR9I CCNGG 3 cut(s) 369, 427, 476
MvaI CCWGG 2 cut(s) 369, 476
MwoI GCNNNNNNNGC 2 cut(s) 171, 592
NciI CCSGG 1 cut(s) 427
NdeII GATC 4 cut(s) 115, 409, 445, 523
NlaIII CATG 3 cut(s) 98, 377, 446
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 1 cut(s) 98
PaeI GCATGC 1 cut(s) 98
PaeR7I CTCGAG 1 cut(s) 519
PagI TCATGA 1 cut(s) 442
PfeI GAWTC 3 cut(s) 53, 209, 215
PfoI TCCNGGA 2 cut(s) 367, 474
PkrI GCNGC 5 cut(s) 167, 170, 173, 400, 594
PsiI TTATAA 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 367, 474
PspGI CCWGG 2 cut(s) 367, 474
PsuI RGATCY 2 cut(s) 115, 523
RsaI GTAC 2 cut(s) 324, 361
RsaNI GTAC 2 cut(s) 323, 360
RseI CAYNNNNRTG 1 cut(s) 462
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 5 cut(s) 166, 169, 172, 399, 593
Sau3AI GATC 4 cut(s) 115, 409, 445, 523
ScaI AGTACT 2 cut(s) 324, 361
ScrFI CCNGG 3 cut(s) 369, 427, 476
SetI ASST 4 cut(s) 153, 347, 498, 520
SfaNI GCATC 1 cut(s) 454
Sfr274I CTCGAG 1 cut(s) 519
SlaI CTCGAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 3 cut(s) 497, 519, 587
SmoI CTYRAG 3 cut(s) 497, 519, 587
SpeI ACTAGT 1 cut(s) 176
SphI GCATGC 1 cut(s) 98
Sse9I AATT 3 cut(s) 106, 289, 379
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 177, 230
StyD4I CCNGG 3 cut(s) 367, 425, 474
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 3 cut(s) 333, 520, 545
TasI AATT 3 cut(s) 106, 289, 379
TatI WGTACW 2 cut(s) 322, 359
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 3 cut(s) 53, 209, 215
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 4 cut(s) 321, 399, 408, 437
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 4 cut(s) 165, 171, 398, 592
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 3 cut(s) 54, 297, 375
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 4 cut(s) 321, 399, 408, 437
XapI RAATTY 2 cut(s) 106, 289
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 177, 230
ZrmI AGTACT 2 cut(s) 324, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.