pycom111g00540

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0016909
Physical Location & Seq
Forward (+)
382323 .. 384767
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00540.2

Sequence Viewer

Length: 1611 bp
ATGATGATCAATACTCATTGTGGAAGCATGGGGTCTATCAAGAGTGAGAACTCATGCAGCTCCGACCTCAGACCCCCGTCTCCGACCGAATCCACGACGATGTCTATGTCCATATCAGAATCTAAGAAAATCACAATTCCTCCATCCTCCGAGTTGGATCAGCAGAGCACCCTAACCCCGCCGAGCCTCAATTTTCCAACGCTCAAGTTCGAGCTAGACGATGTTGAAGTCCAATCTCCCGACCATTCCATCTGGGAGTCTATGTTCTCCGAGCAATTCGATACCGATTTTATGGTCTTATCGCCCGTCCCAAACAACATGCCGAACTCTCCCCAAATCGGCGGTTCAGCAACTCATCAGAATTACAATTACAATTACGCTCAGTCCATGCAAGGTCAGAGCCTCTCGGGGTCCTCTTCCCCTAGGTTTTCGCCGCACCTCGGAGCATTCAGCAGCACCCATAACCCTAAAGGCAAAGGACTCAGCCCGCTCCGTAGGGTTTTCAACTCTCCTGTTAACCAGTACATGGAGCCGGCCGATCAGAACACGCAGTCACTCCCTGTTCCTGTTTCCATCGAAGACTTCTTGGACGATTATCAACGTGACGGATTCATAGCCGCATTCCCAACAACATCATCGTCCAAGATATCAGGAAGCTCGTCACAGTCTTACAATCATCACGATCACTTGCCAGCATCATCGACAGTTCCATCCGCCACGTTGGACTGCGTACCGATGTATAACTCTTCGAGGTTTAGTAGATCGGCGAGTGAAGCGTCGTTGGTTACGGCTGGTAATTCTCAGCTGACACAAGAGAGAAGCAGCGGTAGCGGTTATCAGCAGATGAGCGGCGCGCCACTTTCACAACAATTGCAACAAGAACGCATGCAAGAGAAGCAGCAACAGCCGCAAAAACAACACCACCACCAACCCCAACAACAACAGAACCTTCATTATAATATGATGGCGCCTGCCATCCCTATGGAACAAGATAGCGGGCTCCAACTGCTTCACCTCCTTCTTGTTTGCGCCGAGGCGGTTGCAAAAGAAGACTACATGTTAGCAAGAAGGTACCTCCACCACCTGAACCGAGTGGTGACGCCACTCGGCGATTCCATGCAGCGTGTTGCTTCTTGCTTCACAGATGCCCTAAGCGCACGCCTGGCCGCCACTCTAACCACAAGCCCTAGGGCTTCCGCCCCGCAACCCTTCTCTCCGTTCCCTCCAAACTGGCTTGAAATTCTCAAAATCTACCAAATCGTCTACCAAGCCTGCCCCTACATCAAATTCGCACACTTCACCGCCAACCAAGCCATCTTCGAGGCGTTCGAGTCTGAAGAACGTGTACACGTCATCGACCTAGATATTCTTCAAGGATATCAGTGGCCGGCTTTCATGCAGGCCTTAGCTGCCCGCCCCGGTGGCGCTCCCTTTCTCCGCATAACAGGGGTGGGCCCCTGCATAGAGGCTGTGAAAGAGACGGGCCGGTGTTTGACAGAACTAGCCCTATCTCTTCACGTCCCGTTTGAGTTCCATTCAGTTGGGGAGCAACTTGAAGATCGATCAAGCCACACATGTTCAACCGGAGGATTGGCGAAGCGCTGGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

537

Amino Acids

59.21

Weight (kDa)

5.77

Isoelectric Point (pI)

72.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016909)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53920
malus_domestica MD09G1007200.v1.1 MD17G1011100.v1.1
prunus_persica Prupe.3G309800_v2.0.a1
pyrus_communis pycom111g00540
rosa_chinensis RchiOBHm_Chr2g0176081
rosa_laevigata RLG00000022379
rosa_multiflora Rmu_sc0009879.1_g000008
rosa_rugosa Rorug02G0590300
rosa_samantha Rh2AG669100 Rh2BG680700 Rh2CG643600 Rh2DG694400
rosa_wichuraiana Rw2G054880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 957
Acc65I GGTACC 1 cut(s) 1071
AccB1I GGYRCC 2 cut(s) 967, 1071
AccB7I CCANNNNNTGG 1 cut(s) 526
AccBSI CCGCTC 2 cut(s) 490, 849
AccI GTMKAC 1 cut(s) 1263
AccII CGCG 1 cut(s) 854
AclWI GGATC 1 cut(s) 165
AcoI YGGCCR 3 cut(s) 534, 1164, 1385
AcsI RAATTY 2 cut(s) 1239, 1286
AcuI CTGAAG 1 cut(s) 1356
AcyI GRCGYC 2 cut(s) 968, 1100
AfaI GTAC 4 cut(s) 524, 732, 1073, 1347
AfeI AGCGCT 1 cut(s) 1601
AfiI CCNNNNNNNGG 3 cut(s) 338, 440, 526
AflIII ACRYGT 4 cut(s) 1056, 1342, 1348, 1574
AgsI TTSAA 6 cut(s) 227, 505, 1238, 1373, 1556, 1581
AjiI CACGTC 2 cut(s) 1351, 1519
AjnI CCWGG 1 cut(s) 1161
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 5 cut(s) 60, 214, 657, 805, 1409
AluI AGCT 5 cut(s) 60, 214, 657, 805, 1409
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 84, 1472
AlwI GGATC 1 cut(s) 165
Ama87I CYCGRG 1 cut(s) 406
Aor51HI AGCGCT 1 cut(s) 1601
AoxI GGCC 6 cut(s) 534, 1164, 1385, 1401, 1453, 1483
ApaI GGGCCC 1 cut(s) 1457
ApeKI GCWGC 6 cut(s) 57, 453, 822, 898, 1120, 1409
ApoI RAATTY 2 cut(s) 1239, 1286
AscI GGCGCGCC 1 cut(s) 852
Asp718I GGTACC 1 cut(s) 1071
AspA2I CCTAGG 2 cut(s) 422, 1187
AspLEI GCGC 7 cut(s) 854, 856, 970, 1031, 1157, 1427, 1602
AspS9I GGNCC 4 cut(s) 411, 1453, 1454, 1483
AsuC2I CCSGG 1 cut(s) 1419
AsuHPI GGTGA 3 cut(s) 1004, 1108, 1291
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 411
AvrII CCTAGG 2 cut(s) 422, 1187
BaeGI GKGCMC 1 cut(s) 1457
BanI GGYRCC 2 cut(s) 967, 1071
BanII GRGCYC 2 cut(s) 1002, 1457
BarI GAAGNNNNNNTAC 2 cut(s) 1329, 1361
BbsI GAAGAC 2 cut(s) 585, 1056
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 6 cut(s) 69, 465, 834, 910, 1132, 1396
BccI CCATC 7 cut(s) 151, 257, 581, 718, 958, 983, 1322
BceAI ACGGC 1 cut(s) 804
BcgI CGANNNNNNTGC 2 cut(s) 1022, 1056
BciT130I CCWGG 1 cut(s) 1163
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 1 cut(s) 1419
BcoDI GTCTC 2 cut(s) 84, 1472
BfaI CTAG 5 cut(s) 215, 423, 1188, 1361, 1502
BfoI RGCGCY 3 cut(s) 971, 1428, 1603
BglI GCCNNNNNGGC 1 cut(s) 1422
BlnI CCTAGG 2 cut(s) 422, 1187
Bme1390I CCNGG 2 cut(s) 1163, 1419
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 406
BmgBI CACGTC 2 cut(s) 1351, 1519
BmgT120I GGNCC 4 cut(s) 411, 1453, 1454, 1483
BmiI GGNNCC 7 cut(s) 412, 531, 969, 1001, 1073, 1455, 1456
BmrFI CCNGG 2 cut(s) 1163, 1419
BmsI GCATC 2 cut(s) 704, 1135
BoxI GACNNNNGTC 1 cut(s) 76
BpiI GAAGAC 2 cut(s) 585, 1056
Bpu10I CCTNAGC 2 cut(s) 1151, 1405
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 1419
Bsa29I ATCGAT 1 cut(s) 1561
BsaHI GRCGYC 2 cut(s) 968, 1100
BsaJI CCNNGG 5 cut(s) 422, 439, 1032, 1187, 1417
BsaWI WCCGGW 1 cut(s) 1583
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 338, 440, 526
Bse118I RCCGGY 3 cut(s) 532, 1387, 1485
Bse1I ACTGG 2 cut(s) 520, 1235
BseBI CCWGG 1 cut(s) 1163
BseCI ATCGAT 1 cut(s) 1561
BseDI CCNNGG 5 cut(s) 422, 439, 1032, 1187, 1417
BseGI GGATG 3 cut(s) 143, 710, 975
BseLI CCNNNNNNNGG 3 cut(s) 338, 440, 526
BseMII CTCAG 4 cut(s) 82, 395, 496, 815
BseNI ACTGG 2 cut(s) 520, 1235
BsePI GCGCGC 1 cut(s) 852
BseSI GKGCMC 1 cut(s) 1457
BseX3I CGGCCG 1 cut(s) 534
BseXI GCAGC 6 cut(s) 69, 465, 834, 910, 1132, 1396
Bsh1236I CGCG 1 cut(s) 854
Bsh1285I CGRYCG 2 cut(s) 87, 537
BshFI GGCC 6 cut(s) 536, 1166, 1387, 1403, 1455, 1485
BshNI GGYRCC 2 cut(s) 967, 1071
BshVI ATCGAT 1 cut(s) 1561
BsiEI CGRYCG 2 cut(s) 87, 537
BsiHKAI GWGCWC 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 5 cut(s) 533, 1388, 1419, 1486, 1584
BslFI GGGAC 2 cut(s) 293, 1505
BslI CCNNNNNNNGG 3 cut(s) 338, 440, 526
BsmAI GTCTC 2 cut(s) 84, 1472
BsmBI CGTCTC 2 cut(s) 84, 1472
BsmFI GGGAC 2 cut(s) 293, 1505
BsmI GAATGC 2 cut(s) 446, 620
BsnI GGCC 6 cut(s) 536, 1166, 1387, 1403, 1455, 1485
BsoBI CYCGRG 1 cut(s) 406
Bsp120I GGGCCC 1 cut(s) 1453
Bsp1286I GDGCHC 3 cut(s) 170, 1002, 1457
Bsp1407I TGTACA 1 cut(s) 1345
Bsp143I GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
BspANI GGCC 6 cut(s) 536, 1166, 1387, 1403, 1455, 1485
BspCNI CTCAG 4 cut(s) 81, 394, 495, 814
BspDI ATCGAT 1 cut(s) 1561
BspFNI CGCG 1 cut(s) 854
BspLI GGNNCC 7 cut(s) 412, 531, 969, 1001, 1073, 1455, 1456
BspPI GGATC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 967, 1071
BsrBI CCGCTC 2 cut(s) 490, 849
BsrFI RCCGGY 3 cut(s) 532, 1387, 1485
BsrGI TGTACA 1 cut(s) 1345
BsrI ACTGG 2 cut(s) 520, 1235
BssAI RCCGGY 3 cut(s) 532, 1387, 1485
BssECI CCNNGG 5 cut(s) 422, 439, 1032, 1187, 1417
BssHII GCGCGC 1 cut(s) 852
BssMI GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
BssNI GRCGYC 2 cut(s) 968, 1100
BssT1I CCWWGG 2 cut(s) 422, 1187
Bst2UI CCWGG 1 cut(s) 1163
Bst4CI ACNGT 2 cut(s) 666, 706
Bst6I CTCTTC 3 cut(s) 421, 751, 1518
BstACI GRCGYC 2 cut(s) 968, 1100
BstAUI TGTACA 1 cut(s) 1345
BstDEI CTNAG 7 cut(s) 68, 123, 381, 482, 801, 1151, 1405
BstF5I GGATG 3 cut(s) 143, 710, 975
BstFNI CGCG 1 cut(s) 854
BstH2I RGCGCY 3 cut(s) 971, 1428, 1603
BstHHI GCGC 7 cut(s) 854, 856, 970, 1031, 1157, 1427, 1602
BstKTI GATC 7 cut(s) 9, 160, 541, 685, 764, 1561, 1565
BstMAI GTCTC 2 cut(s) 84, 1472
BstMBI GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
BstMCI CGRYCG 2 cut(s) 87, 537
BstNI CCWGG 1 cut(s) 1163
BstNSI RCATGY 4 cut(s) 322, 889, 1060, 1578
BstPAI GACNNNNGTC 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 1161, 1417
BstSLI GKGCMC 1 cut(s) 1457
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 6 cut(s) 69, 465, 834, 910, 1132, 1396
BstV2I GAAGAC 2 cut(s) 585, 1056
BstXI CCANNNNNNTGG 2 cut(s) 982, 1541
BstZI CGGCCG 1 cut(s) 534
Bsu15I ATCGAT 1 cut(s) 1561
BsuRI GGCC 6 cut(s) 536, 1166, 1387, 1403, 1455, 1485
BsuTUI ATCGAT 1 cut(s) 1561
BtrI CACGTC 2 cut(s) 1351, 1519
BtsCI GGATG 3 cut(s) 143, 710, 975
BtsIMutI CAGTG 1 cut(s) 1388
CfoI GCGC 7 cut(s) 854, 856, 970, 1031, 1157, 1427, 1602
Cfr10I RCCGGY 3 cut(s) 532, 1387, 1485
Cfr13I GGNCC 4 cut(s) 411, 1453, 1454, 1483
ClaI ATCGAT 1 cut(s) 1561
CseI GACGC 2 cut(s) 765, 1108
Csp6I GTAC 4 cut(s) 523, 731, 1072, 1346
CviQI GTAC 4 cut(s) 523, 731, 1072, 1346
DdeI CTNAG 7 cut(s) 68, 123, 381, 482, 801, 1151, 1405
DinI GGCGCC 1 cut(s) 969
DpnI GATC 7 cut(s) 8, 159, 540, 684, 763, 1560, 1564
DpnII GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
EaeI YGGCCR 3 cut(s) 534, 1164, 1385
EagI CGGCCG 1 cut(s) 534
Eam1104I CTCTTC 3 cut(s) 421, 751, 1518
EarI CTCTTC 3 cut(s) 421, 751, 1518
EciI GGCGGA 2 cut(s) 703, 1186
EclXI CGGCCG 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 422, 1187
Eco147I AGGCCT 1 cut(s) 1403
Eco24I GRGCYC 2 cut(s) 1002, 1457
Eco32I GATATC 2 cut(s) 648, 1379
Eco47I GGWCC 1 cut(s) 411
Eco47III AGCGCT 1 cut(s) 1601
Eco52I CGGCCG 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 406
EcoO109I RGGNCCY 2 cut(s) 411, 1454
EcoRII CCWGG 1 cut(s) 1161
EcoRV GATATC 2 cut(s) 648, 1379
EcoT14I CCWWGG 2 cut(s) 422, 1187
EcoT38I GRGCYC 2 cut(s) 1002, 1457
EgeI GGCGCC 1 cut(s) 969
EheI GGCGCC 1 cut(s) 969
ErhI CCWWGG 2 cut(s) 422, 1187
Esp3I CGTCTC 2 cut(s) 84, 1472
FaqI GGGAC 2 cut(s) 293, 1505
FauI CCCGC 5 cut(s) 186, 495, 989, 1209, 1421
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 1263
FokI GGATG 3 cut(s) 130, 697, 962
FriOI GRGCYC 2 cut(s) 1002, 1457
FspBI CTAG 5 cut(s) 215, 423, 1188, 1361, 1502
GlaI GCGC 7 cut(s) 853, 855, 969, 1030, 1156, 1426, 1601
HaeII RGCGCY 3 cut(s) 971, 1428, 1603
HaeIII GGCC 6 cut(s) 536, 1166, 1387, 1403, 1455, 1485
HapII CCGG 5 cut(s) 533, 1388, 1419, 1486, 1584
HgaI GACGC 2 cut(s) 765, 1108
HhaI GCGC 7 cut(s) 854, 856, 970, 1031, 1157, 1427, 1602
Hin1I GRCGYC 2 cut(s) 968, 1100
Hin6I GCGC 7 cut(s) 852, 854, 968, 1029, 1155, 1425, 1600
HinP1I GCGC 7 cut(s) 852, 854, 968, 1029, 1155, 1425, 1600
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 7 cut(s) 89, 119, 257, 480, 609, 1112, 1331
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 5 cut(s) 533, 1388, 1419, 1486, 1584
HphI GGTGA 3 cut(s) 1004, 1108, 1291
Hpy166II GTNNAC 4 cut(s) 517, 1264, 1346, 1348
Hpy188III TCNNGA 4 cut(s) 40, 239, 651, 680
Hpy8I GTNNAC 4 cut(s) 517, 1264, 1346, 1348
Hpy99I CGWCG 2 cut(s) 100, 781
HpyAV CCTTC 4 cut(s) 959, 1028, 1062, 1219
HpyCH4III ACNGT 2 cut(s) 666, 706
HpyCH4IV ACGT 5 cut(s) 601, 719, 1342, 1350, 1518
HpyCH4V TGCA 8 cut(s) 57, 391, 874, 889, 1043, 1120, 1399, 1461
HpyF3I CTNAG 7 cut(s) 68, 123, 381, 482, 801, 1151, 1405
HpySE526I ACGT 5 cut(s) 601, 719, 1342, 1350, 1518
Hsp92I GRCGYC 2 cut(s) 968, 1100
HspAI GCGC 7 cut(s) 852, 854, 968, 1029, 1155, 1425, 1600
KasI GGCGCC 1 cut(s) 967
KpnI GGTACC 1 cut(s) 1075
KroI GCCGGC 2 cut(s) 532, 1387
KroNI GCCGGC 2 cut(s) 534, 1389
Ksp22I TGATCA 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
LmnI GCTCC 7 cut(s) 65, 443, 495, 529, 1005, 1432, 1546
Lsp1109I GCAGC 6 cut(s) 69, 465, 834, 910, 1132, 1396
LweI GCATC 2 cut(s) 704, 1135
MaeI CTAG 5 cut(s) 215, 423, 1188, 1361, 1502
MaeII ACGT 5 cut(s) 601, 719, 1342, 1350, 1518
MaeIII GTNAC 5 cut(s) 552, 602, 660, 784, 1096
MalI GATC 7 cut(s) 8, 159, 540, 684, 763, 1560, 1564
MbiI CCGCTC 2 cut(s) 490, 849
MboI GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
MboII GAAGA 9 cut(s) 408, 590, 738, 1061, 1309, 1349, 1361, 1505, 1568
MfeI CAATTG 1 cut(s) 869
MhlI GDGCHC 3 cut(s) 170, 1002, 1457
Mly113I GGCGCC 1 cut(s) 968
MlyI GAGTC 3 cut(s) 266, 474, 1340
MmeI TCCRAC 6 cut(s) 87, 107, 135, 221, 702, 1027
MroNI GCCGGC 2 cut(s) 532, 1387
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 2 cut(s) 98, 980
MspA1I CMGCKG 2 cut(s) 805, 825
MspI CCGG 5 cut(s) 533, 1388, 1419, 1486, 1584
MspR9I CCNGG 2 cut(s) 1163, 1419
MunI CAATTG 1 cut(s) 869
Mva1269I GAATGC 2 cut(s) 446, 620
MvaI CCWGG 1 cut(s) 1163
MvnI CGCG 1 cut(s) 854
NaeI GCCGGC 2 cut(s) 534, 1389
NarI GGCGCC 1 cut(s) 968
NciI CCSGG 1 cut(s) 1419
NdeII GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
NgoMIV GCCGGC 2 cut(s) 532, 1387
NlaIV GGNNCC 7 cut(s) 412, 531, 969, 1001, 1073, 1455, 1456
NmeAIII GCCGAG 3 cut(s) 207, 1057, 1086
NmuCI GTSAC 4 cut(s) 552, 602, 660, 1096
NspI RCATGY 4 cut(s) 322, 889, 1060, 1578
PaeI GCATGC 1 cut(s) 889
PalAI GGCGCGCC 1 cut(s) 852
PauI GCGCGC 1 cut(s) 852
PceI AGGCCT 1 cut(s) 1403
PciI ACATGT 2 cut(s) 1056, 1574
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 1326
PctI GAATGC 2 cut(s) 446, 620
PdiI GCCGGC 2 cut(s) 534, 1389
PfeI GAWTC 4 cut(s) 89, 119, 609, 1112
PflFI GACNNNGTC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 526
PleI GAGTC 3 cut(s) 265, 474, 1339
PluTI GGCGCC 1 cut(s) 971
PpsI GAGTC 3 cut(s) 265, 474, 1339
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 2 cut(s) 1056, 1574
PshAI GACNNNNGTC 1 cut(s) 76
PsiI TTATAA 1 cut(s) 957
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 1161
PspGI CCWGG 1 cut(s) 1161
PspN4I GGNNCC 7 cut(s) 412, 531, 969, 1001, 1073, 1455, 1456
PspOMI GGGCCC 1 cut(s) 1453
PspPI GGNCC 4 cut(s) 411, 1453, 1454, 1483
PspPPI RGGWCCY 1 cut(s) 411
PsyI GACNNNGTC 1 cut(s) 100
PteI GCGCGC 1 cut(s) 852
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 4 cut(s) 524, 732, 1073, 1347
RsaNI GTAC 4 cut(s) 523, 731, 1072, 1346
RseI CAYNNNNRTG 2 cut(s) 98, 980
SaqAI TTAA 1 cut(s) 516
Sau3AI GATC 7 cut(s) 6, 157, 538, 682, 761, 1558, 1562
Sau96I GGNCC 4 cut(s) 411, 1453, 1454, 1483
SchI GAGTC 3 cut(s) 266, 474, 1340
ScrFI CCNGG 2 cut(s) 1163, 1419
SduI GDGCHC 3 cut(s) 170, 1002, 1457
SfaNI GCATC 2 cut(s) 704, 1135
SfoI GGCGCC 1 cut(s) 969
SgsI GGCGCGCC 1 cut(s) 852
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 2 cut(s) 98, 980
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SphI GCATGC 1 cut(s) 889
SseBI AGGCCT 1 cut(s) 1403
SspDI GGCGCC 1 cut(s) 967
SspMI CTAG 5 cut(s) 215, 423, 1188, 1361, 1502
StuI AGGCCT 1 cut(s) 1403
StyD4I CCNGG 2 cut(s) 1161, 1417
StyI CCWWGG 2 cut(s) 422, 1187
TaaI ACNGT 2 cut(s) 666, 706
TaiI ACGT 5 cut(s) 604, 722, 1345, 1353, 1521
TaqI TCGA 9 cut(s) 210, 279, 576, 701, 749, 1320, 1329, 1356, 1561
TaqII GACCGA 1 cut(s) 101
TatI WGTACW 2 cut(s) 522, 1345
TauI GCSGC 5 cut(s) 436, 620, 852, 910, 1169
TfiI GAWTC 4 cut(s) 89, 119, 609, 1112
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 1 cut(s) 1388
TseFI GTSAC 4 cut(s) 552, 602, 660, 1096
TseI GCWGC 6 cut(s) 57, 453, 822, 898, 1120, 1409
Tsp45I GTSAC 4 cut(s) 552, 602, 660, 1096
TspDTI ATGAA 3 cut(s) 601, 941, 1384
TspGWI ACGGA 3 cut(s) 482, 621, 1206
TspRI CASTG 1 cut(s) 1388
Tth111I GACNNNGTC 1 cut(s) 100
Van91I CCANNNNNTGG 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 2 cut(s) 1239, 1286
XceI RCATGY 4 cut(s) 322, 889, 1060, 1578
XmaJI CCTAGG 2 cut(s) 422, 1187
XmiI GTMKAC 1 cut(s) 1263
XspI CTAG 5 cut(s) 215, 423, 1188, 1361, 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.