RLG00000022379

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016909
Physical Location & Seq
Reverse (-)
82974847 .. 82977320
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022379

Sequence Viewer

Length: 2097 bp
ATGGGTTCGATCAAGAGTGAGAACTCATGCAGCGATTTCAAACCAACTTCTCCCAACGAATCAACAATAATGTCCGAACCACCAAAGAAAGCTACGAGTACTACCACTACTGCTCTGTCTTCCTCCGACATGATGGATCACAGTACTACTAGCTTAACCCCACCGAGCCTCAACTTCCCAGCTCTCAAATTCGAGCTAGAAGGCGACGTCGAAGTTGAATCACCAGACCATTCCATATGGGAGTCGTTGTTTTCTGAACAATTTGATACTGATTTCATGGTGTTGTCCCCGGTGGCTAACACGAACAATAATCTACCCAACTCTCCTCGTACTTTGGGTTACAATGGTCAGGCGATTCAGGGACAGACTAGCTTTTCGGGCTGCTCTCCTCCTCGCTTTGGTTCACCTCCGCTCGGAGCTTTCAGCAGGGACAACAGCAAAGGAAAGGGACTAAGTCCACTCCGGAGGGTTTTCAACTCTCCCGTGAATTACATACAGCAAGAGCCGGAGAACCTGCAGTCACTACCACCAGCGTCAATCGAAGACTTCTTGGATGACTATCAAAGAGACGGCTTTGTAGCAGCGTATAGTGACCCTAACCTTAACCCAACAACCACATGCACATCAAAGATGTCAGGAGGAGGAAGCAATTTGTCCTCGCAATCTTATCACAACCCAATGGTTTCAATGGATTGCAACATGTCAACAACTATGCAAAACCCTTCCAGGTTTTCGCGATCGGTGAGCGAAACATCGTTAGGTACGGCGTGTAACTCTCAGATGACCCAAGAGTCGAGGGGTGGTGGAGCATATCAGCAGATGGGATCCAACTGTGGTGCACCGTTATCACAACAGTTGATGCACGAACGCCAGCAAGAGAAGCAACACATGCAGATGCACCCACAAAAGCAACACCACAACAACCACAATTACAATATGATGGCGCCTTCTCTTCCTCCTGTAGCACCTGAGCCGGAACAAGACAGTGGTCTCCAACTAGTTCATCTCCTTCTTGCTTGTGCCGAAGCTGTTGCCAAAGAAGATTACATGTTAGCAAGAAGGTATCTCCACCACCTCAATCAAGTGGTGTCGGCTCTTGGCGACTCCATGCAACGAGTTGCCGCCTGCTTCACAGAAGCCTTAAGTGCACGCCTTGCCGCAACTCTAACCACCAATCCAAGTGCTTTGGCGCCCAAGCCCTTCTCTCCTTTCCCTCCCAACTCCCTAGAAATCCTCAAGATATACCAAATTGTCTACCAAGCCTGCCCCTATGTCAAATTCGCTCACTTCACCGCTAACCAAGCCATTTTCGAGGCGTTTGAGTCCGAAGAACGTGTGCATGTTATTGACCTCGATATTCTTCAAGGTTATCAATGGCCAGCTTTCATGCAAGCCCTAGCAGCCCGATCGGGCGGTGCTCCATTTCTCCGAATAACTGGGGTCGGTACATGCTTAGAGGCGGTGAAAGAGACGGGCCGGTGTTTGACCGAATTAGCCATCTCTCTTCACGTCCCCTTCGAGTTTCATGCAGTTGGAGAACAACTAGAAGACCTCAAACCACACATGTTCAACCGAAGAATAGGCGAAGCCCTAGCAGTGAACACCGTCAACCGTCTCCACCGCGTTCCGGGGAACTGTCTCGGCAACTTATTAGCCATGATCCGTGACCAAGCACCCAACATTGTGACCCTAGTGGAACAAGAGGCCAGCCACAATGGACCCTACTTTTTGGGCAGGTTCCTCGAAGCCCTACACTATTACTCAGCGATTTTCGACTCGTTGGACGCCACGTTCCCACCGGACTCGGCGCAAAGGGCGAAGGTGGAGCAGTACATATTTGCGCCGGAGATACGGAACATTGTAGCATGTGAAGGAGCGGAGAGGACGGAGAGGCACGAGAGGCTTGAGAAGTGGAGGAAAATAATGGAAGGGAAGGGTTTCAAAAGCGTGCCACTGAGTGCGAACGCGGTGACTCAATCGAAGATATTGCTGGGTTTGTACTCGTGCGACGGCTATAGAATGACGGAGGACAAAGGTTGCTTGCTGTTGGGGTGGCAAGACCGGTCTATTATGGCGGCGTCTGCATGGAGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

699

Amino Acids

77.05

Weight (kDa)

5.74

Isoelectric Point (pI)

59.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 333 - 697 1.1e-132 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016909)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53920
malus_domestica MD09G1007200.v1.1 MD17G1011100.v1.1
prunus_persica Prupe.3G309800_v2.0.a1
pyrus_communis pycom111g00540
rosa_chinensis RchiOBHm_Chr2g0176081
rosa_laevigata RLG00000022379
rosa_multiflora Rmu_sc0009879.1_g000008
rosa_rugosa Rorug02G0590300
rosa_samantha Rh2AG669100 Rh2BG680700 Rh2CG643600 Rh2DG694400
rosa_wichuraiana Rw2G054880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 790
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 2 cut(s) 522, 1725
AccB1I GGYRCC 2 cut(s) 943, 1189
AccBSI CCGCTC 2 cut(s) 412, 1877
AccI GTMKAC 1 cut(s) 1254
AccII CGCG 3 cut(s) 736, 1623, 1967
AccIII TCCGGA 1 cut(s) 462
AclWI GGATC 4 cut(s) 144, 819, 832, 1654
AcoI YGGCCR 1 cut(s) 1376
AcsI RAATTY 2 cut(s) 188, 1277
AcyI GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
AdeI CACNNNGTG 1 cut(s) 1958
AfaI GTAC 7 cut(s) 100, 145, 331, 763, 1447, 1832, 2000
AfiI CCNNNNNNNGG 3 cut(s) 398, 413, 1627
AflII CTTAAG 1 cut(s) 1141
AflIII ACRYGT 4 cut(s) 699, 1047, 1333, 1563
AgeI ACCGGT 1 cut(s) 2061
AgsI TTSAA 7 cut(s) 40, 218, 475, 687, 1364, 1570, 1942
AhlI ACTAGT 1 cut(s) 997
AjiI CACGTC 1 cut(s) 1510
AjnI CCWGG 1 cut(s) 725
AloI GAACNNNNNNTCC 2 cut(s) 1775, 1807
AluBI AGCT 8 cut(s) 92, 153, 182, 196, 372, 419, 1028, 1382
AluI AGCT 8 cut(s) 92, 153, 182, 196, 372, 419, 1028, 1382
Alw21I GWGCWC 3 cut(s) 841, 1150, 1420
Alw26I GTCTC 5 cut(s) 561, 995, 1463, 1619, 1643
Alw44I GTGCAC 2 cut(s) 837, 1146
AlwI GGATC 4 cut(s) 144, 819, 832, 1654
Aor13HI TCCGGA 1 cut(s) 462
AoxI GGCC 3 cut(s) 1376, 1474, 1704
ApaLI GTGCAC 2 cut(s) 837, 1146
ApeKI GCWGC 4 cut(s) 30, 381, 581, 1400
ApoI RAATTY 2 cut(s) 188, 1277
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AsiGI ACCGGT 1 cut(s) 2061
Asp700I GAANNNNTTC 1 cut(s) 1937
AspLEI GCGC 4 cut(s) 946, 1192, 1810, 1843
AspS9I GGNCC 2 cut(s) 1474, 1718
AsuC2I CCSGG 2 cut(s) 290, 1629
AsuHPI GGTGA 6 cut(s) 213, 396, 754, 1282, 1474, 1981
AvaII GGWCC 1 cut(s) 1718
BaeGI GKGCMC 2 cut(s) 841, 1150
BalI TGGCCA 1 cut(s) 1378
BamHI GGATCC 1 cut(s) 824
BanI GGYRCC 2 cut(s) 943, 1189
BauI CACGAG 2 cut(s) 1895, 2002
BbsI GAAGAC 3 cut(s) 111, 549, 1554
Bbv12I GWGCWC 3 cut(s) 841, 1150, 1420
BbvI GCAGC 4 cut(s) 42, 368, 593, 1412
BccI CCATC 4 cut(s) 127, 814, 934, 1505
BceAI ACGGC 3 cut(s) 586, 780, 2026
BciT130I CCWGG 1 cut(s) 727
BcnI CCSGG 2 cut(s) 290, 1629
BcoDI GTCTC 5 cut(s) 561, 995, 1463, 1619, 1643
BcuI ACTAGT 1 cut(s) 997
BfmI CTRYAG 3 cut(s) 515, 960, 2014
BfoI RGCGCY 2 cut(s) 947, 1193
BfrI CTTAAG 1 cut(s) 1141
BfuAI ACCTGC 2 cut(s) 522, 1725
BisI GCNGC 7 cut(s) 31, 382, 582, 1122, 1158, 1401, 2076
BlsI GCNGC 7 cut(s) 32, 383, 583, 1123, 1159, 1402, 2077
BmcAI AGTACT 2 cut(s) 100, 145
Bme1390I CCNGG 3 cut(s) 290, 727, 1629
Bme18I GGWCC 1 cut(s) 1718
BmgBI CACGTC 1 cut(s) 1510
BmgT120I GGNCC 2 cut(s) 1474, 1718
BmiI GGNNCC 5 cut(s) 826, 945, 1191, 1720, 1739
BmrFI CCNGG 3 cut(s) 290, 727, 1629
BmrI ACTGGG 1 cut(s) 1446
BmsI GCATC 3 cut(s) 849, 885, 2081
BmuI ACTGGG 1 cut(s) 1446
BoxI GACNNNNGTC 1 cut(s) 987
BpiI GAAGAC 3 cut(s) 111, 549, 1554
Bpu10I CCTNAGC 1 cut(s) 969
BpuEI CTTGAG 2 cut(s) 1220, 1925
BpuMI CCSGG 2 cut(s) 290, 1629
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 2 cut(s) 288, 1628
BsaWI WCCGGW 3 cut(s) 462, 1798, 2061
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 413, 1627
Bse118I RCCGGY 2 cut(s) 1476, 2061
Bse1I ACTGG 1 cut(s) 1441
Bse8I GATNNNNATC 1 cut(s) 558
BseAI TCCGGA 1 cut(s) 462
BseBI CCWGG 1 cut(s) 727
BseDI CCNNGG 2 cut(s) 288, 1628
BseGI GGATG 1 cut(s) 559
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 3 cut(s) 398, 413, 1627
BseMII CTCAG 4 cut(s) 791, 960, 1776, 1946
BseNI ACTGG 1 cut(s) 1441
BseRI GAGGAG 4 cut(s) 315, 378, 381, 654
BseSI GKGCMC 2 cut(s) 841, 1150
BseXI GCAGC 4 cut(s) 42, 368, 593, 1412
BseYI CCCAGC 2 cut(s) 178, 1990
Bsh1236I CGCG 3 cut(s) 736, 1623, 1967
Bsh1285I CGRYCG 2 cut(s) 740, 1409
BshFI GGCC 3 cut(s) 1378, 1476, 1706
BshNI GGYRCC 2 cut(s) 943, 1189
BshTI ACCGGT 1 cut(s) 2061
BsiEI CGRYCG 2 cut(s) 740, 1409
BsiHKAI GWGCWC 3 cut(s) 841, 1150, 1420
BsiSI CCGG 9 cut(s) 290, 463, 506, 974, 1477, 1628, 1799, 1844, 2062
BslFI GGGAC 5 cut(s) 271, 375, 443, 462, 1496
BslI CCNNNNNNNGG 3 cut(s) 398, 413, 1627
BsmAI GTCTC 5 cut(s) 561, 995, 1463, 1619, 1643
BsmBI CGTCTC 3 cut(s) 561, 1463, 1619
BsmFI GGGAC 5 cut(s) 271, 375, 443, 462, 1496
BsnI GGCC 3 cut(s) 1378, 1476, 1706
Bso31I GGTCTC 1 cut(s) 995
Bsp1286I GDGCHC 3 cut(s) 841, 1150, 1420
Bsp13I TCCGGA 1 cut(s) 462
Bsp143I GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
Bsp68I TCGCGA 1 cut(s) 736
BspANI GGCC 3 cut(s) 1378, 1476, 1706
BspCNI CTCAG 4 cut(s) 790, 961, 1775, 1947
BspEI TCCGGA 1 cut(s) 462
BspFNI CGCG 3 cut(s) 736, 1623, 1967
BspLI GGNNCC 5 cut(s) 826, 945, 1191, 1720, 1739
BspMAI CTGCAG 1 cut(s) 519
BspMI ACCTGC 2 cut(s) 522, 1725
BspPI GGATC 4 cut(s) 144, 819, 832, 1654
BspT107I GGYRCC 2 cut(s) 943, 1189
BspTI CTTAAG 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 995
BsrBI CCGCTC 2 cut(s) 412, 1877
BsrFI RCCGGY 2 cut(s) 1476, 2061
BsrI ACTGG 1 cut(s) 1441
BssAI RCCGGY 2 cut(s) 1476, 2061
BssECI CCNNGG 2 cut(s) 288, 1628
BssMI GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
BssNI GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
BssSI CACGAG 2 cut(s) 1895, 2002
Bst2BI CACGAG 2 cut(s) 1895, 2002
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 8 cut(s) 143, 833, 843, 855, 986, 1606, 1613, 1637
Bst6I CTCTTC 2 cut(s) 957, 1509
BstACI GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
BstAFI CTTAAG 1 cut(s) 1141
BstAPI GCANNNNNTGC 2 cut(s) 889, 1154
BstC8I GCNNGC 9 cut(s) 872, 1126, 1150, 1264, 1380, 1392, 1708, 1949, 2042
BstDEI CTNAG 6 cut(s) 452, 777, 969, 1453, 1762, 1955
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 3 cut(s) 736, 1623, 1967
BstH2I RGCGCY 2 cut(s) 947, 1193
BstHHI GCGC 4 cut(s) 946, 1192, 1810, 1843
BstKTI GATC 6 cut(s) 12, 139, 740, 827, 1409, 1662
BstMAI GTCTC 5 cut(s) 561, 995, 1463, 1619, 1643
BstMBI GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
BstMCI CGRYCG 2 cut(s) 740, 1409
BstNI CCWGG 1 cut(s) 727
BstNSI RCATGY 8 cut(s) 621, 703, 892, 1051, 1343, 1452, 1567, 1869
BstPAI GACNNNNGTC 1 cut(s) 987
BstSCI CCNGG 3 cut(s) 288, 725, 1627
BstSFI CTRYAG 3 cut(s) 515, 960, 2014
BstSLI GKGCMC 2 cut(s) 841, 1150
BstUI CGCG 3 cut(s) 736, 1623, 1967
BstV1I GCAGC 4 cut(s) 42, 368, 593, 1412
BstV2I GAAGAC 3 cut(s) 111, 549, 1554
BstX2I RGATCY 1 cut(s) 824
BstYI RGATCY 1 cut(s) 824
BsuRI GGCC 3 cut(s) 1378, 1476, 1706
BtrI CACGTC 1 cut(s) 1510
BtsCI GGATG 1 cut(s) 559
BtsI GCAGTG 1 cut(s) 1602
BtsIMutI CAGTG 3 cut(s) 991, 1602, 1952
BtuMI TCGCGA 1 cut(s) 736
BveI ACCTGC 2 cut(s) 522, 1725
Cac8I GCNNGC 9 cut(s) 872, 1126, 1150, 1264, 1380, 1392, 1708, 1949, 2042
CfoI GCGC 4 cut(s) 946, 1192, 1810, 1843
Cfr10I RCCGGY 2 cut(s) 1476, 2061
Cfr13I GGNCC 2 cut(s) 1474, 1718
CseI GACGC 3 cut(s) 522, 1793, 2067
Csp6I GTAC 7 cut(s) 99, 144, 330, 762, 1446, 1831, 1999
CspAI ACCGGT 1 cut(s) 2061
CviQI GTAC 7 cut(s) 99, 144, 330, 762, 1446, 1831, 1999
DdeI CTNAG 6 cut(s) 452, 777, 969, 1453, 1762, 1955
DinI GGCGCC 2 cut(s) 945, 1191
DpnI GATC 6 cut(s) 11, 138, 739, 826, 1408, 1661
DpnII GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
DraIII CACNNNGTG 1 cut(s) 1958
DrdI GACNNNNNNGTC 1 cut(s) 790
DseDI GACNNNNNNGTC 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 1376
Eam1104I CTCTTC 2 cut(s) 957, 1509
EarI CTCTTC 2 cut(s) 957, 1509
Eco31I GGTCTC 1 cut(s) 995
Eco47I GGWCC 1 cut(s) 1718
EcoRII CCWGG 1 cut(s) 725
EgeI GGCGCC 2 cut(s) 945, 1191
EheI GGCGCC 2 cut(s) 945, 1191
Esp3I CGTCTC 3 cut(s) 561, 1463, 1619
FaqI GGGAC 5 cut(s) 271, 375, 443, 462, 1496
FauNDI CATATG 1 cut(s) 236
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 7 cut(s) 31, 382, 582, 1122, 1158, 1401, 2076
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 7 cut(s) 31, 382, 582, 1122, 1158, 1401, 2076
GlaI GCGC 4 cut(s) 945, 1191, 1809, 1842
GluI GCNGC 7 cut(s) 31, 382, 582, 1122, 1158, 1401, 2076
GsaI CCCAGC 2 cut(s) 182, 1994
HaeII RGCGCY 2 cut(s) 947, 1193
HaeIII GGCC 3 cut(s) 1378, 1476, 1706
HapII CCGG 9 cut(s) 290, 463, 506, 974, 1477, 1628, 1799, 1844, 2062
HgaI GACGC 3 cut(s) 522, 1793, 2067
HhaI GCGC 4 cut(s) 946, 1192, 1810, 1843
Hin1I GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
Hin6I GCGC 4 cut(s) 944, 1190, 1808, 1841
HinP1I GCGC 4 cut(s) 944, 1190, 1808, 1841
HincII GTYRAC 2 cut(s) 705, 1609
HindII GTYRAC 2 cut(s) 705, 1609
HpaII CCGG 9 cut(s) 290, 463, 506, 974, 1477, 1628, 1799, 1844, 2062
HphI GGTGA 6 cut(s) 213, 396, 754, 1282, 1474, 1981
Hpy166II GTNNAC 8 cut(s) 404, 458, 705, 839, 1148, 1255, 1600, 1609
Hpy188I TCNGA 7 cut(s) 76, 127, 256, 416, 780, 1327, 1430
Hpy188III TCNNGA 5 cut(s) 13, 463, 636, 735, 1237
Hpy8I GTNNAC 8 cut(s) 404, 458, 705, 839, 1148, 1255, 1600, 1609
Hpy99I CGWCG 3 cut(s) 209, 212, 2012
HpyCH4III ACNGT 8 cut(s) 143, 833, 843, 855, 986, 1606, 1613, 1637
HpyCH4IV ACGT 4 cut(s) 207, 1333, 1509, 1790
HpyF3I CTNAG 6 cut(s) 452, 777, 969, 1453, 1762, 1955
HpySE526I ACGT 4 cut(s) 207, 1333, 1509, 1790
Hsp92I GRCGYC 5 cut(s) 207, 944, 1190, 1785, 2078
HspAI GCGC 4 cut(s) 944, 1190, 1808, 1841
KasI GGCGCC 2 cut(s) 943, 1189
Kpn2I TCCGGA 1 cut(s) 462
Kzo9I GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
LmnI GCTCC 5 cut(s) 416, 806, 1423, 1825, 1874
Lsp1109I GCAGC 4 cut(s) 42, 368, 593, 1412
LweI GCATC 3 cut(s) 849, 885, 2081
MaeII ACGT 4 cut(s) 207, 1333, 1509, 1790
MaeIII GTNAC 7 cut(s) 338, 519, 590, 770, 1664, 1684, 1969
MalI GATC 6 cut(s) 11, 138, 739, 826, 1408, 1661
MbiI CCGCTC 2 cut(s) 412, 1877
MboI GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
MflI RGATCY 1 cut(s) 824
MhlI GDGCHC 3 cut(s) 841, 1150, 1420
MlsI TGGCCA 1 cut(s) 1378
MluCI AATT 8 cut(s) 188, 260, 487, 649, 928, 1248, 1277, 1490
MluNI TGGCCA 1 cut(s) 1378
Mly113I GGCGCC 2 cut(s) 944, 1190
MlyI GAGTC 7 cut(s) 251, 800, 1097, 1331, 1769, 1796, 1966
MmeI TCCRAC 5 cut(s) 150, 852, 1018, 1513, 1761
Mox20I TGGCCA 1 cut(s) 1378
MroI TCCGGA 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 1937
MscI TGGCCA 1 cut(s) 1378
MseI TTAA 3 cut(s) 155, 603, 1142
MslI CAYNNNNRTG 2 cut(s) 893, 2089
Msp20I TGGCCA 1 cut(s) 1378
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 9 cut(s) 290, 463, 506, 974, 1477, 1628, 1799, 1844, 2062
MspR9I CCNGG 3 cut(s) 290, 727, 1629
MvaI CCWGG 1 cut(s) 727
MvnI CGCG 3 cut(s) 736, 1623, 1967
NarI GGCGCC 2 cut(s) 944, 1190
NciI CCSGG 2 cut(s) 290, 1629
NdeI CATATG 1 cut(s) 236
NdeII GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
NlaIV GGNNCC 5 cut(s) 826, 945, 1191, 1720, 1739
NmeAIII GCCGAG 2 cut(s) 1620, 1784
NmuCI GTSAC 5 cut(s) 519, 590, 1664, 1684, 1969
NruI TCGCGA 1 cut(s) 736
NspI RCATGY 8 cut(s) 621, 703, 892, 1051, 1343, 1452, 1567, 1869
PciI ACATGT 3 cut(s) 699, 1047, 1563
PcsI WCGNNNNNNNCGW 1 cut(s) 1515
PdmI GAANNNNTTC 1 cut(s) 1937
PfeI GAWTC 3 cut(s) 59, 218, 355
PflFI GACNNNGTC 1 cut(s) 453
PinAI ACCGGT 1 cut(s) 2061
PkrI GCNGC 7 cut(s) 32, 383, 583, 1123, 1159, 1402, 2077
Ple19I CGATCG 2 cut(s) 740, 1409
PleI GAGTC 7 cut(s) 250, 799, 1097, 1330, 1769, 1796, 1966
PluTI GGCGCC 2 cut(s) 947, 1193
PpsI GAGTC 7 cut(s) 250, 799, 1097, 1330, 1769, 1796, 1966
PscI ACATGT 3 cut(s) 699, 1047, 1563
PshAI GACNNNNGTC 1 cut(s) 987
Psp6I CCWGG 1 cut(s) 725
PspFI CCCAGC 2 cut(s) 178, 1990
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 5 cut(s) 826, 945, 1191, 1720, 1739
PspPI GGNCC 2 cut(s) 1474, 1718
PstI CTGCAG 1 cut(s) 519
PsuI RGATCY 1 cut(s) 824
PsyI GACNNNGTC 1 cut(s) 453
PvuI CGATCG 2 cut(s) 740, 1409
RruI TCGCGA 1 cut(s) 736
RsaI GTAC 7 cut(s) 100, 145, 331, 763, 1447, 1832, 2000
RsaNI GTAC 7 cut(s) 99, 144, 330, 762, 1446, 1831, 1999
RseI CAYNNNNRTG 2 cut(s) 893, 2089
SaqAI TTAA 3 cut(s) 155, 603, 1142
SatI GCNGC 7 cut(s) 31, 382, 582, 1122, 1158, 1401, 2076
Sau3AI GATC 6 cut(s) 9, 136, 737, 824, 1406, 1659
Sau96I GGNCC 2 cut(s) 1474, 1718
ScaI AGTACT 2 cut(s) 100, 145
SchI GAGTC 7 cut(s) 251, 800, 1097, 1331, 1769, 1796, 1966
ScrFI CCNGG 3 cut(s) 290, 727, 1629
SduI GDGCHC 3 cut(s) 841, 1150, 1420
SfaNI GCATC 3 cut(s) 849, 885, 2081
SfcI CTRYAG 3 cut(s) 515, 960, 2014
SfoI GGCGCC 2 cut(s) 945, 1191
SinI GGWCC 1 cut(s) 1718
SmiMI CAYNNNNRTG 2 cut(s) 893, 2089
SmlI CTYRAG 3 cut(s) 1141, 1235, 1904
SmoI CTYRAG 3 cut(s) 1141, 1235, 1904
SpeI ACTAGT 1 cut(s) 997
Sse9I AATT 8 cut(s) 188, 260, 487, 649, 928, 1248, 1277, 1490
SspDI GGCGCC 2 cut(s) 943, 1189
StyD4I CCNGG 3 cut(s) 288, 725, 1627
TaaI ACNGT 8 cut(s) 143, 833, 843, 855, 986, 1606, 1613, 1637
TaiI ACGT 4 cut(s) 210, 1336, 1512, 1793
TaqII GACCGA 1 cut(s) 1502
TasI AATT 8 cut(s) 188, 260, 487, 649, 928, 1248, 1277, 1490
TatI WGTACW 4 cut(s) 98, 143, 1830, 1998
TauI GCSGC 3 cut(s) 1124, 1160, 2078
TfiI GAWTC 3 cut(s) 59, 218, 355
Tru1I TTAA 3 cut(s) 155, 603, 1142
Tru9I TTAA 3 cut(s) 155, 603, 1142
TscAI CASTG 3 cut(s) 991, 1602, 1959
TseFI GTSAC 5 cut(s) 519, 590, 1664, 1684, 1969
TseI GCWGC 4 cut(s) 30, 381, 581, 1400
Tsp45I GTSAC 5 cut(s) 519, 590, 1664, 1684, 1969
TspDTI ATGAA 4 cut(s) 265, 992, 1375, 1514
TspGWI ACGGA 4 cut(s) 1652, 1867, 1901, 2039
TspRI CASTG 3 cut(s) 991, 1602, 1959
Tth111I GACNNNGTC 1 cut(s) 453
Vha464I CTTAAG 1 cut(s) 1141
VneI GTGCAC 2 cut(s) 837, 1146
VpaK11BI GGWCC 1 cut(s) 1718
XapI RAATTY 2 cut(s) 188, 1277
XceI RCATGY 8 cut(s) 621, 703, 892, 1051, 1343, 1452, 1567, 1869
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 1 cut(s) 1937
ZraI GACGTC 1 cut(s) 208
ZrmI AGTACT 2 cut(s) 100, 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.