Rw2G054880

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016909
Physical Location & Seq
Reverse (-)
82464085 .. 82466581
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054880.1

Sequence Viewer

Length: 2088 bp
ATGATGATGAACTCTCATTGTGGGAGCATGGGTTCGATCAAGAGTGAGAACTCATGCAGCGATTTCAAACCAACTTCTCCCAACGAATCAACAATAATGTCCGAACCACCAAAGAAAGCTACGAGTACTACCACTACTGCTCTGTCTTCCTCCGACATGATGGATCACAGTACTACTAGCTTAACCCCACCGAGCCTCAACTTCCCAGCTCTCAAATTCGAGCTAGAAGGCGACGTCGAAGTTGAATCACCAGACCATTCCATATGGGAGTCGTTGTTTTCGGAACAATTTGATACTGATTTCATGGTGTTGTCCCCGGTGGCAAACACGAACAATAATCTACCCAACTCTCCTCGTACTTTCGGTTACAATGGTCAGGCGATTCAGGGACAGACTAGCTTTTCGGGCTGCTCTCCTCCTCGCTTTGGTTCACCTCCTCTCGGAGCTTTCAGCAGGGACAACAGCAAAGGAAAGGGACTAAGTCCACTCCGGAGGGTTTTCAACTCTCCCGTGAATTACATACAGCAAGAGCCGGAGAACCTGCAGTCACTACCACCAGCGTCAATCGAAGACTTCTTGGATGACTATCAAAGAGACGGCTTTGTAGCAGCGTATAGTGACCCTAACCTTAACCCAACAACCACATGCACATCAAAGATGTCAGGAGGAGGAAGCAATTTGTCCTCGCAATCTTATCACAACCCAATGGTTTCAATGGATTGCAACATGTCAACAACCATGCAAAACCCTTCCAGGTTTTCGCGATCGGTGAGCGAAACATCGTTAGTTACGGCGTGTAACTCTCAGATGACCCAAGAGTCGAGGGGGGGTGGAGCATATCAGCAGATGGGATCCAACTGTAGTGCACCAGAAGCAACACATGCAGATGCACCCCAAAAGCAACACCACAACAACCACAATTACAATTTGATGGCGCCTTCTCTTCCTCCTGTTGCACCTGAGCCGGAACAAGACAGTGGTCTCCAACTGGTTCATCTCCTTCTTGCTTGTGCAGAAGCTGTTGCGAAAGAAGATTACATGTTAGCAAGAAGGTATCTCCACCACCTCAATCAAGTGGTGTCGGCTCTTGGCGACTCCATGCAACGTGTTGCCGCCTGCTTCACAGAAGCCTTAAGCGCACGCCTTGCCGCAACTCTAACCACCAATCCAAGTGCTTTGGCTCCCAAGCCCTTCTCTCCTTTCCCTCCCAACTCCCTAGAAATCCTCAAGATATACCAAATTGTCTACCAAGCCTGCCCCTACGTCAAATTCGCTCACTTCACCGCTAACCAAGCCATTTTCGAGGCGTTTGAGTCCGAAGAACGTGTGCATGTTATTGACCTCGATATTCTTCAAGGTTATCAATGGCCAGCTTTCATGCAAGCCCTAGCAGCCCGATCGGGCGGTGCCCCATTTCTCCGAATAACTGGAGTCGGTCCATGCTTAGAGGCGGTGAAAGAGACGGGCCGGTGTTTGACTGAATTAGCAATCTCTCTTCACGTCCCCTTTGAGTTTCATGCAGTTGGAGAACAACTAGAAGACCTCAAACCACACATGTTCAACCGAAGAATAGGCGAAGCCCTAGCAGTGAACACCGTGAACCGTCTCCACCGCGTTCCGGGGAACTGTCTCGGCAACTTATTAGCCATGATCCGTGACCAAGCACCCAACATTGTGACCCTAGTGGAACAAGAGGCCAGCCACAATGGACCCTACTTTTTGGGCAGGTTCCTCGAAGCCCTACATTATTACTCGGCGATTTTCGACTCCTTGGACGCCACGTTCCCACCGGACTCGGCGCAAAGGGCTAAGGTGGAGCAGTACATATTTGCGCCGGAGATACGGAACATTGTAGCATGTGAAGGAGCGGAGAGGACGGAGAGGCACGAGAGGCTGGAGAAGTGGAGGAAAATAATGGAAGGGAAGGGTTTCAAAAGCGTGCCACTGAGTGCGAACGCGGTGACTCAGTCGAAGATATTGCTGGGTTTGTACTCGTGCGACGGCTATAGAATGACGGAGGACAAAGGTTGCTTGCTGTTGGGGTGGCAAGACCGGTCTATTATGGCGGCGTCTGCATGGAGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

695

Amino Acids

76.38

Weight (kDa)

5.59

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 330 - 694 1e-132 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016909)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53920
malus_domestica MD09G1007200.v1.1 MD17G1011100.v1.1
prunus_persica Prupe.3G309800_v2.0.a1
pyrus_communis pycom111g00540
rosa_chinensis RchiOBHm_Chr2g0176081
rosa_laevigata RLG00000022379
rosa_multiflora Rmu_sc0009879.1_g000008
rosa_rugosa Rorug02G0590300
rosa_samantha Rh2AG669100 Rh2BG680700 Rh2CG643600 Rh2DG694400
rosa_wichuraiana Rw2G054880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 817
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 2 cut(s) 549, 1716
AccB1I GGYRCC 2 cut(s) 934, 1406
AccBSI CCGCTC 1 cut(s) 1868
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 3 cut(s) 763, 1614, 1958
AccIII TCCGGA 1 cut(s) 489
AciI CCGC 9 cut(s) 1113, 1149, 1284, 1404, 1451, 1612, 1868, 1958, 2066
AclWI GGATC 4 cut(s) 171, 846, 859, 1645
AcoI YGGCCR 1 cut(s) 1367
AcsI RAATTY 2 cut(s) 215, 1268
AcyI GRCGYC 4 cut(s) 234, 935, 1776, 2069
AdeI CACNNNGTG 1 cut(s) 1949
AfaI GTAC 5 cut(s) 127, 172, 358, 1823, 1991
AfiI CCNNNNNNNGG 3 cut(s) 425, 440, 1618
AflII CTTAAG 1 cut(s) 1132
AflIII ACRYGT 5 cut(s) 726, 1038, 1105, 1324, 1554
AgeI ACCGGT 1 cut(s) 2052
AgsI TTSAA 7 cut(s) 67, 245, 502, 714, 1355, 1561, 1933
AjiI CACGTC 1 cut(s) 1501
AjnI CCWGG 1 cut(s) 752
AloI GAACNNNNNNTCC 4 cut(s) 16, 48, 1766, 1798
AluBI AGCT 8 cut(s) 119, 180, 209, 223, 399, 446, 1019, 1373
AluI AGCT 8 cut(s) 119, 180, 209, 223, 399, 446, 1019, 1373
Alw21I GWGCWC 1 cut(s) 868
Alw26I GTCTC 5 cut(s) 588, 986, 1454, 1610, 1634
Alw44I GTGCAC 1 cut(s) 864
AlwI GGATC 4 cut(s) 171, 846, 859, 1645
AlwNI CAGNNNCTG 1 cut(s) 1019
Aor13HI TCCGGA 1 cut(s) 489
AoxI GGCC 3 cut(s) 1367, 1465, 1695
ApaLI GTGCAC 1 cut(s) 864
ApeKI GCWGC 4 cut(s) 57, 408, 608, 1391
ApoI RAATTY 2 cut(s) 215, 1268
ArsI GACNNNNNNTTYG 2 cut(s) 385, 417
AsiGI ACCGGT 1 cut(s) 2052
Asp700I GAANNNNTTC 1 cut(s) 1928
AspLEI GCGC 4 cut(s) 937, 1139, 1801, 1834
AspS9I GGNCC 3 cut(s) 1436, 1465, 1709
AsuC2I CCSGG 2 cut(s) 317, 1620
AsuHPI GGTGA 6 cut(s) 240, 423, 781, 1273, 1465, 1972
AvaII GGWCC 2 cut(s) 1436, 1709
BaeGI GKGCMC 2 cut(s) 868, 1411
BalI TGGCCA 1 cut(s) 1369
BamHI GGATCC 1 cut(s) 851
BanI GGYRCC 2 cut(s) 934, 1406
BauI CACGAG 2 cut(s) 1886, 1993
BbsI GAAGAC 3 cut(s) 138, 576, 1545
Bbv12I GWGCWC 1 cut(s) 868
BbvI GCAGC 4 cut(s) 69, 395, 620, 1403
BccI CCATC 3 cut(s) 154, 841, 925
BceAI ACGGC 3 cut(s) 613, 807, 2017
BcgI CGANNNNNNTGC 6 cut(s) 1380, 1414, 1714, 1748, 1960, 1994
BciT130I CCWGG 1 cut(s) 754
BcnI CCSGG 2 cut(s) 317, 1620
BcoDI GTCTC 5 cut(s) 588, 986, 1454, 1610, 1634
BfaI CTAG 9 cut(s) 177, 224, 396, 1217, 1388, 1535, 1583, 1682, 2086
BfmI CTRYAG 3 cut(s) 542, 859, 2005
BfoI RGCGCY 1 cut(s) 938
BfrI CTTAAG 1 cut(s) 1132
BfuAI ACCTGC 2 cut(s) 549, 1716
BisI GCNGC 7 cut(s) 58, 409, 609, 1113, 1149, 1392, 2067
BlsI GCNGC 7 cut(s) 59, 410, 610, 1114, 1150, 1393, 2068
BmcAI AGTACT 2 cut(s) 127, 172
Bme1390I CCNGG 3 cut(s) 317, 754, 1620
Bme18I GGWCC 2 cut(s) 1436, 1709
BmgBI CACGTC 1 cut(s) 1501
BmgT120I GGNCC 3 cut(s) 1436, 1465, 1709
BmiI GGNNCC 6 cut(s) 853, 936, 1182, 1408, 1711, 1730
BmrFI CCNGG 3 cut(s) 317, 754, 1620
BmsI GCATC 2 cut(s) 877, 2072
BoxI GACNNNNGTC 1 cut(s) 978
BpiI GAAGAC 3 cut(s) 138, 576, 1545
BpmI CTGGAG 2 cut(s) 1449, 1916
Bpu10I CCTNAGC 2 cut(s) 960, 1809
BpuEI CTTGAG 1 cut(s) 1211
BpuMI CCSGG 2 cut(s) 317, 1620
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 4 cut(s) 234, 935, 1776, 2069
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 3 cut(s) 315, 1619, 1770
BsaWI WCCGGW 3 cut(s) 489, 1789, 2052
BsaXI ACNNNNNCTCC 4 cut(s) 16, 46, 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 440, 1618
Bse118I RCCGGY 2 cut(s) 1467, 2052
Bse1I ACTGG 2 cut(s) 993, 1432
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 489
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 3 cut(s) 315, 1619, 1770
BseGI GGATG 1 cut(s) 586
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 425, 440, 1618
BseMII CTCAG 4 cut(s) 818, 951, 1937, 1979
BseNI ACTGG 2 cut(s) 993, 1432
BseRI GAGGAG 5 cut(s) 342, 405, 408, 426, 681
BseSI GKGCMC 2 cut(s) 868, 1411
BseXI GCAGC 4 cut(s) 69, 395, 620, 1403
BseYI CCCAGC 2 cut(s) 205, 1981
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 3 cut(s) 763, 1614, 1958
Bsh1285I CGRYCG 2 cut(s) 767, 1400
BshFI GGCC 3 cut(s) 1369, 1467, 1697
BshNI GGYRCC 2 cut(s) 934, 1406
BshTI ACCGGT 1 cut(s) 2052
BsiEI CGRYCG 2 cut(s) 767, 1400
BsiHKAI GWGCWC 1 cut(s) 868
BsiSI CCGG 9 cut(s) 317, 490, 533, 965, 1468, 1619, 1790, 1835, 2053
BslFI GGGAC 5 cut(s) 298, 402, 470, 489, 1487
BslI CCNNNNNNNGG 3 cut(s) 425, 440, 1618
BsmAI GTCTC 5 cut(s) 588, 986, 1454, 1610, 1634
BsmBI CGTCTC 3 cut(s) 588, 1454, 1610
BsmFI GGGAC 5 cut(s) 298, 402, 470, 489, 1487
BsnI GGCC 3 cut(s) 1369, 1467, 1697
Bso31I GGTCTC 1 cut(s) 986
Bsp1286I GDGCHC 2 cut(s) 868, 1411
Bsp13I TCCGGA 1 cut(s) 489
Bsp143I GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
Bsp68I TCGCGA 1 cut(s) 763
BspACI CCGC 9 cut(s) 1113, 1149, 1284, 1404, 1451, 1612, 1868, 1958, 2066
BspANI GGCC 3 cut(s) 1369, 1467, 1697
BspCNI CTCAG 4 cut(s) 817, 952, 1938, 1978
BspEI TCCGGA 1 cut(s) 489
BspFNI CGCG 3 cut(s) 763, 1614, 1958
BspLI GGNNCC 6 cut(s) 853, 936, 1182, 1408, 1711, 1730
BspMAI CTGCAG 1 cut(s) 546
BspMI ACCTGC 2 cut(s) 549, 1716
BspPI GGATC 4 cut(s) 171, 846, 859, 1645
BspT107I GGYRCC 2 cut(s) 934, 1406
BspTI CTTAAG 1 cut(s) 1132
BspTNI GGTCTC 1 cut(s) 986
BsrBI CCGCTC 1 cut(s) 1868
BsrFI RCCGGY 2 cut(s) 1467, 2052
BsrI ACTGG 2 cut(s) 993, 1432
BssAI RCCGGY 2 cut(s) 1467, 2052
BssECI CCNNGG 3 cut(s) 315, 1619, 1770
BssMI GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
BssNI GRCGYC 4 cut(s) 234, 935, 1776, 2069
BssSI CACGAG 2 cut(s) 1886, 1993
BssT1I CCWWGG 1 cut(s) 1770
Bst2BI CACGAG 2 cut(s) 1886, 1993
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 6 cut(s) 170, 860, 977, 1597, 1604, 1628
Bst6I CTCTTC 2 cut(s) 948, 1500
BstACI GRCGYC 4 cut(s) 234, 935, 1776, 2069
BstAFI CTTAAG 1 cut(s) 1132
BstAPI GCANNNNNTGC 2 cut(s) 881, 1145
BstC8I GCNNGC 8 cut(s) 1117, 1141, 1255, 1371, 1383, 1699, 1940, 2033
BstDEI CTNAG 7 cut(s) 479, 804, 960, 1444, 1809, 1946, 1965
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 3 cut(s) 763, 1614, 1958
BstH2I RGCGCY 1 cut(s) 938
BstHHI GCGC 4 cut(s) 937, 1139, 1801, 1834
BstKTI GATC 6 cut(s) 39, 166, 767, 854, 1400, 1653
BstMAI GTCTC 5 cut(s) 588, 986, 1454, 1610, 1634
BstMBI GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
BstMCI CGRYCG 2 cut(s) 767, 1400
BstNI CCWGG 1 cut(s) 754
BstNSI RCATGY 7 cut(s) 648, 730, 884, 1042, 1334, 1558, 1860
BstPAI GACNNNNGTC 1 cut(s) 978
BstSCI CCNGG 3 cut(s) 315, 752, 1618
BstSFI CTRYAG 3 cut(s) 542, 859, 2005
BstSLI GKGCMC 2 cut(s) 868, 1411
BstUI CGCG 3 cut(s) 763, 1614, 1958
BstV1I GCAGC 4 cut(s) 69, 395, 620, 1403
BstV2I GAAGAC 3 cut(s) 138, 576, 1545
BstX2I RGATCY 1 cut(s) 851
BstYI RGATCY 1 cut(s) 851
BsuRI GGCC 3 cut(s) 1369, 1467, 1697
BtrI CACGTC 1 cut(s) 1501
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 1593
BtsIMutI CAGTG 3 cut(s) 982, 1593, 1943
BtuMI TCGCGA 1 cut(s) 763
BveI ACCTGC 2 cut(s) 549, 1716
Cac8I GCNNGC 8 cut(s) 1117, 1141, 1255, 1371, 1383, 1699, 1940, 2033
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 4 cut(s) 937, 1139, 1801, 1834
Cfr10I RCCGGY 2 cut(s) 1467, 2052
Cfr13I GGNCC 3 cut(s) 1436, 1465, 1709
CseI GACGC 3 cut(s) 549, 1784, 2058
Csp6I GTAC 5 cut(s) 126, 171, 357, 1822, 1990
CspAI ACCGGT 1 cut(s) 2052
CviQI GTAC 5 cut(s) 126, 171, 357, 1822, 1990
DdeI CTNAG 7 cut(s) 479, 804, 960, 1444, 1809, 1946, 1965
DinI GGCGCC 1 cut(s) 936
DpnI GATC 6 cut(s) 38, 165, 766, 853, 1399, 1652
DpnII GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
DraIII CACNNNGTG 1 cut(s) 1949
DrdI GACNNNNNNGTC 1 cut(s) 817
DseDI GACNNNNNNGTC 1 cut(s) 817
EaeI YGGCCR 1 cut(s) 1367
Eam1104I CTCTTC 2 cut(s) 948, 1500
EarI CTCTTC 2 cut(s) 948, 1500
Eco130I CCWWGG 1 cut(s) 1770
Eco31I GGTCTC 1 cut(s) 986
Eco47I GGWCC 2 cut(s) 1436, 1709
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 1 cut(s) 1770
EgeI GGCGCC 1 cut(s) 936
EheI GGCGCC 1 cut(s) 936
ErhI CCWWGG 1 cut(s) 1770
Esp3I CGTCTC 3 cut(s) 588, 1454, 1610
FaqI GGGAC 5 cut(s) 298, 402, 470, 489, 1487
FauNDI CATATG 1 cut(s) 263
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 7 cut(s) 58, 409, 609, 1113, 1149, 1392, 2067
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 7 cut(s) 58, 409, 609, 1113, 1149, 1392, 2067
FspBI CTAG 9 cut(s) 177, 224, 396, 1217, 1388, 1535, 1583, 1682, 2086
GlaI GCGC 4 cut(s) 936, 1138, 1800, 1833
GluI GCNGC 7 cut(s) 58, 409, 609, 1113, 1149, 1392, 2067
GsaI CCCAGC 2 cut(s) 209, 1985
GsuI CTGGAG 2 cut(s) 1449, 1916
HaeII RGCGCY 1 cut(s) 938
HaeIII GGCC 3 cut(s) 1369, 1467, 1697
HapII CCGG 9 cut(s) 317, 490, 533, 965, 1468, 1619, 1790, 1835, 2053
HgaI GACGC 3 cut(s) 549, 1784, 2058
HhaI GCGC 4 cut(s) 937, 1139, 1801, 1834
Hin1I GRCGYC 4 cut(s) 234, 935, 1776, 2069
Hin6I GCGC 4 cut(s) 935, 1137, 1799, 1832
HinP1I GCGC 4 cut(s) 935, 1137, 1799, 1832
HincII GTYRAC 1 cut(s) 732
HindII GTYRAC 1 cut(s) 732
HpaII CCGG 9 cut(s) 317, 490, 533, 965, 1468, 1619, 1790, 1835, 2053
HphI GGTGA 6 cut(s) 240, 423, 781, 1273, 1465, 1972
Hpy166II GTNNAC 7 cut(s) 431, 485, 732, 866, 1246, 1591, 1600
Hpy188I TCNGA 7 cut(s) 103, 154, 283, 443, 807, 1318, 1421
Hpy188III TCNNGA 5 cut(s) 40, 490, 663, 762, 1228
Hpy8I GTNNAC 7 cut(s) 431, 485, 732, 866, 1246, 1591, 1600
Hpy99I CGWCG 3 cut(s) 236, 239, 2003
HpyAV CCTTC 9 cut(s) 221, 759, 948, 1010, 1044, 1201, 1856, 1913, 1918
HpyCH4III ACNGT 6 cut(s) 170, 860, 977, 1597, 1604, 1628
HpyCH4IV ACGT 6 cut(s) 234, 1105, 1263, 1324, 1500, 1781
HpyF3I CTNAG 7 cut(s) 479, 804, 960, 1444, 1809, 1946, 1965
HpySE526I ACGT 6 cut(s) 234, 1105, 1263, 1324, 1500, 1781
Hsp92I GRCGYC 4 cut(s) 234, 935, 1776, 2069
HspAI GCGC 4 cut(s) 935, 1137, 1799, 1832
KasI GGCGCC 1 cut(s) 934
Kpn2I TCCGGA 1 cut(s) 489
Kzo9I GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
LmnI GCTCC 6 cut(s) 24, 443, 833, 1186, 1816, 1865
Lsp1109I GCAGC 4 cut(s) 69, 395, 620, 1403
LweI GCATC 2 cut(s) 877, 2072
MaeI CTAG 9 cut(s) 177, 224, 396, 1217, 1388, 1535, 1583, 1682, 2086
MaeII ACGT 6 cut(s) 234, 1105, 1263, 1324, 1500, 1781
MaeIII GTNAC 8 cut(s) 365, 546, 617, 787, 797, 1655, 1675, 1960
MalI GATC 6 cut(s) 38, 165, 766, 853, 1399, 1652
MbiI CCGCTC 1 cut(s) 1868
MboI GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
MflI RGATCY 1 cut(s) 851
MhlI GDGCHC 2 cut(s) 868, 1411
MlsI TGGCCA 1 cut(s) 1369
MluCI AATT 9 cut(s) 215, 287, 514, 676, 919, 925, 1239, 1268, 1481
MluNI TGGCCA 1 cut(s) 1369
Mly113I GGCGCC 1 cut(s) 935
MlyI GAGTC 8 cut(s) 278, 827, 1088, 1322, 1440, 1760, 1787, 1957
MmeI TCCRAC 4 cut(s) 177, 879, 1009, 1504
Mox20I TGGCCA 1 cut(s) 1369
MroI TCCGGA 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 1928
MscI TGGCCA 1 cut(s) 1369
MseI TTAA 3 cut(s) 182, 630, 1133
MslI CAYNNNNRTG 2 cut(s) 885, 2080
Msp20I TGGCCA 1 cut(s) 1369
MspCI CTTAAG 1 cut(s) 1132
MspI CCGG 9 cut(s) 317, 490, 533, 965, 1468, 1619, 1790, 1835, 2053
MspR9I CCNGG 3 cut(s) 317, 754, 1620
MvaI CCWGG 1 cut(s) 754
MvnI CGCG 3 cut(s) 763, 1614, 1958
NarI GGCGCC 1 cut(s) 935
NciI CCSGG 2 cut(s) 317, 1620
NdeI CATATG 1 cut(s) 263
NdeII GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
NlaIV GGNNCC 6 cut(s) 853, 936, 1182, 1408, 1711, 1730
NmeAIII GCCGAG 3 cut(s) 1611, 1733, 1775
NmuCI GTSAC 5 cut(s) 546, 617, 1655, 1675, 1960
NruI TCGCGA 1 cut(s) 763
NspI RCATGY 7 cut(s) 648, 730, 884, 1042, 1334, 1558, 1860
PciI ACATGT 3 cut(s) 726, 1038, 1554
PdmI GAANNNNTTC 1 cut(s) 1928
PfeI GAWTC 3 cut(s) 86, 245, 382
PflFI GACNNNGTC 2 cut(s) 480, 1966
PinAI ACCGGT 1 cut(s) 2052
PkrI GCNGC 7 cut(s) 59, 410, 610, 1114, 1150, 1393, 2068
Ple19I CGATCG 2 cut(s) 767, 1400
PleI GAGTC 8 cut(s) 277, 826, 1088, 1321, 1439, 1760, 1787, 1957
PluTI GGCGCC 1 cut(s) 938
PpsI GAGTC 8 cut(s) 277, 826, 1088, 1321, 1439, 1760, 1787, 1957
PscI ACATGT 3 cut(s) 726, 1038, 1554
PshAI GACNNNNGTC 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 752
PspFI CCCAGC 2 cut(s) 205, 1981
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 6 cut(s) 853, 936, 1182, 1408, 1711, 1730
PspPI GGNCC 3 cut(s) 1436, 1465, 1709
PstI CTGCAG 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 851
PsyI GACNNNGTC 2 cut(s) 480, 1966
PvuI CGATCG 2 cut(s) 767, 1400
RruI TCGCGA 1 cut(s) 763
RsaI GTAC 5 cut(s) 127, 172, 358, 1823, 1991
RsaNI GTAC 5 cut(s) 126, 171, 357, 1822, 1990
RseI CAYNNNNRTG 2 cut(s) 885, 2080
SaqAI TTAA 3 cut(s) 182, 630, 1133
SatI GCNGC 7 cut(s) 58, 409, 609, 1113, 1149, 1392, 2067
Sau3AI GATC 6 cut(s) 36, 163, 764, 851, 1397, 1650
Sau96I GGNCC 3 cut(s) 1436, 1465, 1709
ScaI AGTACT 2 cut(s) 127, 172
SchI GAGTC 8 cut(s) 278, 827, 1088, 1322, 1440, 1760, 1787, 1957
ScrFI CCNGG 3 cut(s) 317, 754, 1620
SduI GDGCHC 2 cut(s) 868, 1411
SfaNI GCATC 2 cut(s) 877, 2072
SfcI CTRYAG 3 cut(s) 542, 859, 2005
SfoI GGCGCC 1 cut(s) 936
SinI GGWCC 2 cut(s) 1436, 1709
SmiMI CAYNNNNRTG 2 cut(s) 885, 2080
SmlI CTYRAG 2 cut(s) 1132, 1226
SmoI CTYRAG 2 cut(s) 1132, 1226
Sse9I AATT 9 cut(s) 215, 287, 514, 676, 919, 925, 1239, 1268, 1481
SsiI CCGC 9 cut(s) 1113, 1149, 1284, 1404, 1451, 1612, 1868, 1958, 2066
SspDI GGCGCC 1 cut(s) 934
SspMI CTAG 9 cut(s) 177, 224, 396, 1217, 1388, 1535, 1583, 1682, 2086
StyD4I CCNGG 3 cut(s) 315, 752, 1618
StyI CCWWGG 1 cut(s) 1770
TaaI ACNGT 6 cut(s) 170, 860, 977, 1597, 1604, 1628
TaiI ACGT 6 cut(s) 237, 1108, 1266, 1327, 1503, 1784
TaqII GACCGA 1 cut(s) 1424
TasI AATT 9 cut(s) 215, 287, 514, 676, 919, 925, 1239, 1268, 1481
TatI WGTACW 4 cut(s) 125, 170, 1821, 1989
TauI GCSGC 3 cut(s) 1115, 1151, 2069
TfiI GAWTC 3 cut(s) 86, 245, 382
Tru1I TTAA 3 cut(s) 182, 630, 1133
Tru9I TTAA 3 cut(s) 182, 630, 1133
TscAI CASTG 3 cut(s) 982, 1593, 1950
TseFI GTSAC 5 cut(s) 546, 617, 1655, 1675, 1960
TseI GCWGC 4 cut(s) 57, 408, 608, 1391
Tsp45I GTSAC 5 cut(s) 546, 617, 1655, 1675, 1960
TspDTI ATGAA 5 cut(s) 23, 292, 983, 1366, 1505
TspGWI ACGGA 4 cut(s) 1643, 1858, 1892, 2030
TspRI CASTG 3 cut(s) 982, 1593, 1950
Tth111I GACNNNGTC 2 cut(s) 480, 1966
Vha464I CTTAAG 1 cut(s) 1132
VneI GTGCAC 1 cut(s) 864
VpaK11BI GGWCC 2 cut(s) 1436, 1709
XapI RAATTY 2 cut(s) 215, 1268
XceI RCATGY 7 cut(s) 648, 730, 884, 1042, 1334, 1558, 1860
XmiI GTMKAC 1 cut(s) 1245
XmnI GAANNNNTTC 1 cut(s) 1928
XspI CTAG 9 cut(s) 177, 224, 396, 1217, 1388, 1535, 1583, 1682, 2086
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 2 cut(s) 127, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.