Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0013284

Physical Location & Seq

Genomic Coordinates Reverse (-)

1323143 .. 1326421

Gene Structure 3279 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGAAGACGTCGATGACAGAGCTCGACGACGAAACGGTGCGCAGCATGGCCATTGGCGCCGTTTTCTCGGACTTCGGTGGGAAGATAAATTCGCTTGATTTTCATCGAAAGGACGATTTACTAGTTACTGCTAGCGAGGATGATTCTGTTCGACTCTATGACATTGCAACTGCTAAGTTGATAAAGACTACATTTCATAAGAAACATGGTGCAGATCGTATATGCTTTACCCATCACCCAAGTTCTGTTATATGCTCTTCAAAACACAATCTGGATACCACCGGAGAATCGCTGCGGTATCTGTCGATGTATGATAATCGATGCCTTCGCTATTTCAAAGGACATAAAGAGAGGATTGTCTCCCTCTGTATGTCTCCGGTGAATGATAGCTTCATGTCTGGTTCATTGGACCACACCGTCAGAATATGGGATCTCCGTGTAAATGCCTGCCAGGGAATTTTACATATGCGTGGTAGACCTACAGTTGCCTATGACCAGCAAGGTCTTGTTTTTGCCGTGGCAATGGAAGGGGGTGCGATTAAATTGTTTGACTCACGCTCCTATGACAAGGGTCCATTTGACACCTTTTTAGTTGGTGGAGATACTGCTGAAGTTTGTGATATCAAATTTAGCAACGATGGCAAATCAATGTTATTGACAACCACAAACAACAATATCTATGTTCTTGATGCATATGGAGGGGAGAAGCGTTGTGGGTACAGCGTGGAACCCTCTCTGAATGTAATTATCGAGGCAACTTTTACCCCAGATGGCCAGTATGTGGTGTCAGGCTCTGGAGATGGAAATTTGCACGCCTGGAGCATCAACAACCGAAACGAGGTGGCATGCTGGGACAGTCACATCGGAGTTCCGTCATGCTTGAAGTGGGCTCCTCGCAGAGCCATGTTTGCCGCCGCATCAAGCGTTCTCACGTTTTGGATACCTAATGAAGCAAACGAGCCTACTCACATGGAGACCGAAGCTGGTGCTGCCGATCCCGAGCATCCTTCCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009987 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019538 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0034708 GO:0034968 GO:0035097 GO:0036211 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048188 GO:0048506 GO:0048509 GO:0048510 GO:0048580 GO:0048583 GO:0048586 GO:0050789 GO:0050793 GO:0051239 GO:0051276 GO:0051568 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080182 GO:1901564 GO:1902494 GO:1990234 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

339

Amino Acids

37.4

Weight (kDa)

5.69

Isoelectric Point (pI)

44.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Prp19	PF24814	20 - 220	1.7e-20	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	25 - 90	6.1e-07	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	26 - 148	8.2e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	28 - 308	2.4e-36	WDR5 beta-propeller domain
WDR55	PF24796	28 - 150	1.4e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	29 - 309	1.8e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	97 - 230	2.3e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	101 - 150	6.2e-07	WDR3 first beta-propeller domain
WD40	PF00400	107 - 144	3.3e-08	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	109 - 183	7.9e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML	PF23409	110 - 294	8.5e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	111 - 273	3.3e-10	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	111 - 307	4e-23	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	113 - 230	7.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	121 - 280	1.6e-17	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	161 - 286	3.5e-09	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	162 - 301	1.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	162 - 305	2.2e-09	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14530
fragaria_vesca	FvH4_6g51320 FvH4_6g51320
malus_domestica	MD09G1028100.v1.1
prunus_persica	Prupe.3G290600_v2.0.a1 Prupe.3G290600_v2.0.a1
pyrus_communis	pycom111g02200
rosa_chinensis	RchiOBHm_Chr2g0172941
rosa_laevigata	RLG00000022144
rosa_multiflora	Rmu_sc0029209.1_g000002 Rmu_sc0029210.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079400
rosa_rugosa	Rorug02G0563800
rosa_samantha	Rh2AG640600 Rh2BG654000 Rh2CG619700 Rh2DG667700
rosa_wichuraiana	Rw2G052880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	416
AatII	GACGTC	1 cut(s)	11
Acc16I	TGCGCA	1 cut(s)	41
AccB1I	GGYRCC	1 cut(s)	56
AccB7I	CCANNNNNTGG	1 cut(s)	781
AccI	GTMKAC	1 cut(s)	475
AciI	CCGC	3 cut(s)	295, 912, 915
AclWI	GGATC	2 cut(s)	438, 989
AcoI	YGGCCR	2 cut(s)	48, 772
AcsI	RAATTY	4 cut(s)	88, 456, 626, 805
AcuI	CTGAAG	1 cut(s)	630
AcyI	GRCGYC	2 cut(s)	8, 57
AfaI	GTAC	1 cut(s)	719
AfiI	CCNNNNNNNGG	2 cut(s)	781, 838
AgsI	TTSAA	3 cut(s)	261, 337, 883
AhlI	ACTAGT	1 cut(s)	121
AjnI	CCWGG	2 cut(s)	450, 815
AluBI	AGCT	3 cut(s)	22, 390, 983
AluI	AGCT	3 cut(s)	22, 390, 983
Alw21I	GWGCWC	1 cut(s)	24
Alw26I	GTCTC	3 cut(s)	364, 378, 968
AlwI	GGATC	2 cut(s)	438, 989
AlwNI	CAGNNNCTG	1 cut(s)	794
Ama87I	CYCGRG	1 cut(s)	998
AoxI	GGCC	2 cut(s)	48, 772
ApeKI	GCWGC	3 cut(s)	42, 292, 989
ApoI	RAATTY	4 cut(s)	88, 456, 626, 805
AspLEI	GCGC	2 cut(s)	42, 59
AspS9I	GGNCC	2 cut(s)	409, 572
AsuHPI	GGTGA	2 cut(s)	227, 391
AsuNHI	GCTAGC	1 cut(s)	131
AvaI	CYCGRG	1 cut(s)	998
AvaII	GGWCC	2 cut(s)	409, 572
BalI	TGGCCA	2 cut(s)	50, 774
BanI	GGYRCC	1 cut(s)	56
BanII	GRGCYC	2 cut(s)	24, 892
BbsI	GAAGAC	1 cut(s)	11
Bbv12I	GWGCWC	1 cut(s)	24
BbvI	GCAGC	3 cut(s)	54, 279, 976
BccI	CCATC	4 cut(s)	240, 632, 764, 794
BceAI	ACGGC	2 cut(s)	44, 500
BcgI	CGANNNNNNTGC	2 cut(s)	968, 1002
BciT130I	CCWGG	2 cut(s)	452, 817
BciVI	GTATCC	2 cut(s)	268, 933
BcoDI	GTCTC	3 cut(s)	364, 378, 968
BcuI	ACTAGT	1 cut(s)	121
BfaI	CTAG	2 cut(s)	122, 132
BfmI	CTRYAG	1 cut(s)	480
BfoI	RGCGCY	1 cut(s)	60
BfuI	GTATCC	2 cut(s)	268, 933
BisI	GCNGC	5 cut(s)	43, 293, 912, 915, 990
BlsI	GCNGC	5 cut(s)	44, 294, 913, 916, 991
Bme1390I	CCNGG	2 cut(s)	452, 817
Bme18I	GGWCC	2 cut(s)	409, 572
BmeT110I	CYCGRG	1 cut(s)	998
BmgT120I	GGNCC	2 cut(s)	409, 572
BmiI	GGNNCC	4 cut(s)	58, 573, 729, 891
BmrFI	CCNGG	2 cut(s)	452, 817
BmrI	ACTGGG	1 cut(s)	1006
BmsI	GCATC	5 cut(s)	311, 679, 831, 926, 1012
BmtI	GCTAGC	1 cut(s)	135
BmuI	ACTGGG	1 cut(s)	1006
BoxI	GACNNNNGTC	1 cut(s)	570
BpiI	GAAGAC	1 cut(s)	11
BpmI	CTGGAG	2 cut(s)	816, 838
Bsa29I	ATCGAT	1 cut(s)	319
BsaBI	GATNNNNATC	1 cut(s)	102
BsaHI	GRCGYC	2 cut(s)	8, 57
BsaI	GGTCTC	1 cut(s)	968
BsaJI	CCNNGG	2 cut(s)	451, 516
BsaWI	WCCGGW	2 cut(s)	281, 376
BsaXI	ACNNNNNCTCC	2 cut(s)	542, 572
Bsc4I	CCNNNNNNNGG	2 cut(s)	781, 838
Bse1I	ACTGG	2 cut(s)	775, 1012
Bse3DI	GCAATG	2 cut(s)	162, 528
Bse8I	GATNNNNATC	1 cut(s)	102
BseBI	CCWGG	2 cut(s)	452, 817
BseCI	ATCGAT	1 cut(s)	319
BseDI	CCNNGG	2 cut(s)	451, 516
BseGI	GGATG	2 cut(s)	145, 1003
BseJI	GATNNNNATC	1 cut(s)	102
BseLI	CCNNNNNNNGG	2 cut(s)	781, 838
BseMI	GCAATG	2 cut(s)	162, 528
BseNI	ACTGG	2 cut(s)	775, 1012
BseRI	GAGGAG	1 cut(s)	882
BseXI	GCAGC	3 cut(s)	54, 279, 976
BseYI	CCCAGC	1 cut(s)	849
BsgI	GTGCAG	1 cut(s)	231
BshFI	GGCC	2 cut(s)	50, 774
BshNI	GGYRCC	1 cut(s)	56
BshVI	ATCGAT	1 cut(s)	319
BsiHKAI	GWGCWC	1 cut(s)	24
BsiHKCI	CYCGRG	1 cut(s)	998
BsiSI	CCGG	2 cut(s)	282, 377
BslFI	GGGAC	1 cut(s)	866
BslI	CCNNNNNNNGG	2 cut(s)	781, 838
BsmAI	GTCTC	3 cut(s)	364, 378, 968
BsmFI	GGGAC	1 cut(s)	866
BsnI	GGCC	2 cut(s)	50, 774
Bso31I	GGTCTC	1 cut(s)	968
BsoBI	CYCGRG	1 cut(s)	998
Bsp1286I	GDGCHC	2 cut(s)	24, 892
Bsp143I	GATC	3 cut(s)	214, 430, 994
BspACI	CCGC	3 cut(s)	295, 912, 915
BspANI	GGCC	2 cut(s)	50, 774
BspDI	ATCGAT	1 cut(s)	319
BspLI	GGNNCC	4 cut(s)	58, 573, 729, 891
BspOI	GCTAGC	1 cut(s)	135
BspPI	GGATC	2 cut(s)	438, 989
BspQI	GCTCTTC	1 cut(s)	262
BspT107I	GGYRCC	1 cut(s)	56
BspTNI	GGTCTC	1 cut(s)	968
BsrDI	GCAATG	2 cut(s)	162, 528
BsrI	ACTGG	2 cut(s)	775, 1012
BssECI	CCNNGG	2 cut(s)	451, 516
BssMI	GATC	3 cut(s)	214, 430, 994
BssNI	GRCGYC	2 cut(s)	8, 57
Bst2UI	CCWGG	2 cut(s)	452, 817
Bst4CI	ACNGT	4 cut(s)	37, 418, 484, 857
Bst6I	CTCTTC	1 cut(s)	262
BstACI	GRCGYC	2 cut(s)	8, 57
BstC8I	GCNNGC	4 cut(s)	133, 448, 813, 847
BstDEI	CTNAG	1 cut(s)	174
BstDSI	CCRYGG	1 cut(s)	516
BstF5I	GGATG	2 cut(s)	145, 1003
BstH2I	RGCGCY	1 cut(s)	60
BstHHI	GCGC	2 cut(s)	42, 59
BstKTI	GATC	3 cut(s)	217, 433, 997
BstMAI	GTCTC	3 cut(s)	364, 378, 968
BstMBI	GATC	3 cut(s)	214, 430, 994
BstMWI	GCNNNNNNNGC	4 cut(s)	56, 639, 908, 989
BstNI	CCWGG	2 cut(s)	452, 817
BstNSI	RCATGY	1 cut(s)	849
BstPAI	GACNNNNGTC	1 cut(s)	570
BstSCI	CCNGG	2 cut(s)	450, 815
BstSFI	CTRYAG	1 cut(s)	480
BstV1I	GCAGC	3 cut(s)	54, 279, 976
BstV2I	GAAGAC	1 cut(s)	11
BstX2I	RGATCY	1 cut(s)	430
BstYI	RGATCY	1 cut(s)	430
Bsu15I	ATCGAT	1 cut(s)	319
BsuI	GTATCC	2 cut(s)	268, 933
BsuRI	GGCC	2 cut(s)	50, 774
BsuTUI	ATCGAT	1 cut(s)	319
BtgI	CCRYGG	1 cut(s)	516
BtsCI	GGATG	2 cut(s)	145, 1003
Cac8I	GCNNGC	4 cut(s)	133, 448, 813, 847
CaiI	CAGNNNCTG	1 cut(s)	794
CfoI	GCGC	2 cut(s)	42, 59
Cfr13I	GGNCC	2 cut(s)	409, 572
ClaI	ATCGAT	1 cut(s)	319
Csp6I	GTAC	1 cut(s)	718
CviAII	CATG	7 cut(s)	46, 206, 394, 846, 876, 904, 970
CviJI	RGCY	9 cut(s)	22, 50, 390, 774, 792, 890, 902, 961, 983
CviKI_1	RGCY	9 cut(s)	22, 50, 390, 774, 792, 890, 902, 961, 983
CviQI	GTAC	1 cut(s)	718
DdeI	CTNAG	1 cut(s)	174
DinI	GGCGCC	1 cut(s)	58
DpnI	GATC	3 cut(s)	216, 432, 996
DpnII	GATC	3 cut(s)	214, 430, 994
DrdI	GACNNNNNNGTC	1 cut(s)	416
DseDI	GACNNNNNNGTC	1 cut(s)	416
EaeI	YGGCCR	2 cut(s)	48, 772
Eam1104I	CTCTTC	1 cut(s)	262
EarI	CTCTTC	1 cut(s)	262
Ecl136II	GAGCTC	1 cut(s)	22
Eco24I	GRGCYC	2 cut(s)	24, 892
Eco31I	GGTCTC	1 cut(s)	968
Eco32I	GATATC	1 cut(s)	622
Eco47I	GGWCC	2 cut(s)	409, 572
Eco53kI	GAGCTC	1 cut(s)	22
Eco57I	CTGAAG	1 cut(s)	630
Eco88I	CYCGRG	1 cut(s)	998
EcoICRI	GAGCTC	1 cut(s)	22
EcoRII	CCWGG	2 cut(s)	450, 815
EcoRV	GATATC	1 cut(s)	622
EcoT22I	ATGCAT	1 cut(s)	694
EcoT38I	GRGCYC	2 cut(s)	24, 892
EgeI	GGCGCC	1 cut(s)	58
EheI	GGCGCC	1 cut(s)	58
FaeI	CATG	7 cut(s)	49, 209, 397, 849, 879, 907, 973
FaqI	GGGAC	1 cut(s)	866
FatI	CATG	7 cut(s)	45, 205, 393, 845, 875, 903, 969
FauNDI	CATATG	2 cut(s)	465, 694
FblI	GTMKAC	1 cut(s)	475
Fnu4HI	GCNGC	5 cut(s)	43, 293, 912, 915, 990
FokI	GGATG	2 cut(s)	152, 990
FriOI	GRGCYC	2 cut(s)	24, 892
Fsp4HI	GCNGC	5 cut(s)	43, 293, 912, 915, 990
FspBI	CTAG	2 cut(s)	122, 132
FspI	TGCGCA	1 cut(s)	41
GlaI	GCGC	2 cut(s)	41, 58
GluI	GCNGC	5 cut(s)	43, 293, 912, 915, 990
GsaI	CCCAGC	1 cut(s)	853
GsuI	CTGGAG	2 cut(s)	816, 838
HaeII	RGCGCY	1 cut(s)	60
HaeIII	GGCC	2 cut(s)	50, 774
HapII	CCGG	2 cut(s)	282, 377
HhaI	GCGC	2 cut(s)	42, 59
Hin1I	GRCGYC	2 cut(s)	8, 57
Hin1II	CATG	7 cut(s)	49, 209, 397, 849, 879, 907, 973
Hin6I	GCGC	2 cut(s)	40, 57
HinP1I	GCGC	2 cut(s)	40, 57
HinfI	GANTC	4 cut(s)	143, 153, 287, 551
HpaII	CCGG	2 cut(s)	282, 377
HphI	GGTGA	2 cut(s)	227, 391
Hpy166II	GTNNAC	1 cut(s)	476
Hpy188I	TCNGA	4 cut(s)	70, 422, 738, 866
Hpy188III	TCNNGA	4 cut(s)	272, 686, 795, 998
Hpy8I	GTNNAC	1 cut(s)	476
Hpy99I	CGWCG	3 cut(s)	13, 29, 32
HpyAV	CCTTC	3 cut(s)	335, 521, 1017
HpyCH4III	ACNGT	4 cut(s)	37, 418, 484, 857
HpyCH4IV	ACGT	2 cut(s)	8, 932
HpyCH4V	TGCA	4 cut(s)	167, 212, 692, 811
HpyF10VI	GCNNNNNNNGC	4 cut(s)	56, 639, 908, 989
HpyF3I	CTNAG	1 cut(s)	174
HpySE526I	ACGT	2 cut(s)	8, 932
Hsp92I	GRCGYC	2 cut(s)	8, 57
Hsp92II	CATG	7 cut(s)	49, 209, 397, 849, 879, 907, 973
HspAI	GCGC	2 cut(s)	40, 57
KasI	GGCGCC	1 cut(s)	56
Kzo9I	GATC	3 cut(s)	214, 430, 994
LguI	GCTCTTC	1 cut(s)	262
LmnI	GCTCC	3 cut(s)	563, 819, 895
Lsp1109I	GCAGC	3 cut(s)	54, 279, 976
LweI	GCATC	5 cut(s)	311, 679, 831, 926, 1012
MaeI	CTAG	2 cut(s)	122, 132
MaeII	ACGT	2 cut(s)	8, 932
MaeIII	GTNAC	2 cut(s)	124, 857
MalI	GATC	3 cut(s)	216, 432, 996
MboI	GATC	3 cut(s)	214, 430, 994
MboII	GAAGA	3 cut(s)	16, 94, 249
MflI	RGATCY	1 cut(s)	430
MhlI	GDGCHC	2 cut(s)	24, 892
MlsI	TGGCCA	2 cut(s)	50, 774
MluCI	AATT	6 cut(s)	88, 456, 542, 626, 744, 805
MluNI	TGGCCA	2 cut(s)	50, 774
Mly113I	GGCGCC	1 cut(s)	57
MlyI	GAGTC	2 cut(s)	147, 545
MnlI	CCTC	8 cut(s)	130, 345, 374, 692, 742, 745, 832, 903
Mox20I	TGGCCA	2 cut(s)	50, 774
Mph1103I	ATGCAT	1 cut(s)	694
MscI	TGGCCA	2 cut(s)	50, 774
MseI	TTAA	1 cut(s)	540
MslI	CAYNNNNRTG	1 cut(s)	468
Msp20I	TGGCCA	2 cut(s)	50, 774
MspI	CCGG	2 cut(s)	282, 377
MspR9I	CCNGG	2 cut(s)	452, 817
MvaI	CCWGG	2 cut(s)	452, 817
MwoI	GCNNNNNNNGC	4 cut(s)	56, 639, 908, 989
NarI	GGCGCC	1 cut(s)	57
NdeI	CATATG	2 cut(s)	465, 694
NdeII	GATC	3 cut(s)	214, 430, 994
NheI	GCTAGC	1 cut(s)	131
NlaIII	CATG	7 cut(s)	49, 209, 397, 849, 879, 907, 973
NlaIV	GGNNCC	4 cut(s)	58, 573, 729, 891
NmuCI	GTSAC	1 cut(s)	857
NsbI	TGCGCA	1 cut(s)	41
NsiI	ATGCAT	1 cut(s)	694
NspI	RCATGY	1 cut(s)	849
PaeI	GCATGC	1 cut(s)	849
PciSI	GCTCTTC	1 cut(s)	262
PfeI	GAWTC	2 cut(s)	143, 287
PflMI	CCANNNNNTGG	1 cut(s)	781
PkrI	GCNGC	5 cut(s)	44, 294, 913, 916, 991
PleI	GAGTC	2 cut(s)	147, 545
PluTI	GGCGCC	1 cut(s)	60
PpsI	GAGTC	2 cut(s)	147, 545
PshAI	GACNNNNGTC	1 cut(s)	570
Psp124BI	GAGCTC	1 cut(s)	24
Psp6I	CCWGG	2 cut(s)	450, 815
PspFI	CCCAGC	1 cut(s)	849
PspGI	CCWGG	2 cut(s)	450, 815
PspN4I	GGNNCC	4 cut(s)	58, 573, 729, 891
PspPI	GGNCC	2 cut(s)	409, 572
PstNI	CAGNNNCTG	1 cut(s)	794
PsuI	RGATCY	1 cut(s)	430
RsaI	GTAC	1 cut(s)	719
RsaNI	GTAC	1 cut(s)	718
RseI	CAYNNNNRTG	1 cut(s)	468
SacI	GAGCTC	1 cut(s)	24
SapI	GCTCTTC	1 cut(s)	262
SaqAI	TTAA	1 cut(s)	540
SatI	GCNGC	5 cut(s)	43, 293, 912, 915, 990
Sau3AI	GATC	3 cut(s)	214, 430, 994
Sau96I	GGNCC	2 cut(s)	409, 572
SchI	GAGTC	2 cut(s)	147, 545
ScrFI	CCNGG	2 cut(s)	452, 817
SduI	GDGCHC	2 cut(s)	24, 892
SfaNI	GCATC	5 cut(s)	311, 679, 831, 926, 1012
SfcI	CTRYAG	1 cut(s)	480
SfoI	GGCGCC	1 cut(s)	58
SinI	GGWCC	2 cut(s)	409, 572
SmiMI	CAYNNNNRTG	1 cut(s)	468
SpeI	ACTAGT	1 cut(s)	121
SphI	GCATGC	1 cut(s)	849
Sse9I	AATT	6 cut(s)	88, 456, 542, 626, 744, 805
SsiI	CCGC	3 cut(s)	295, 912, 915
SspDI	GGCGCC	1 cut(s)	56
SspMI	CTAG	2 cut(s)	122, 132
SstI	GAGCTC	1 cut(s)	24
StyD4I	CCNGG	2 cut(s)	450, 815
TaaI	ACNGT	4 cut(s)	37, 418, 484, 857
TaiI	ACGT	2 cut(s)	11, 935
TaqI	TCGA	7 cut(s)	11, 24, 106, 151, 305, 319, 750
TaqII	GACCGA	1 cut(s)	992
TasI	AATT	6 cut(s)	88, 456, 542, 626, 744, 805
TauI	GCSGC	2 cut(s)	914, 917
TfiI	GAWTC	2 cut(s)	143, 287
Tru1I	TTAA	1 cut(s)	540
Tru9I	TTAA	1 cut(s)	540
TseFI	GTSAC	1 cut(s)	857
TseI	GCWGC	3 cut(s)	42, 292, 989
Tsp45I	GTSAC	1 cut(s)	857
TspDTI	ATGAA	6 cut(s)	17, 92, 185, 382, 393, 963
TspGWI	ACGGA	2 cut(s)	425, 861
Van91I	CCANNNNNTGG	1 cut(s)	781
VpaK11BI	GGWCC	2 cut(s)	409, 572
XapI	RAATTY	4 cut(s)	88, 456, 626, 805
XceI	RCATGY	1 cut(s)	849
XmiI	GTMKAC	1 cut(s)	475
XspI	CTAG	2 cut(s)	122, 132
ZraI	GACGTC	1 cut(s)	9
Zsp2I	ATGCAT	1 cut(s)	694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.