Rh2BG654000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013284
Physical Location & Seq
Reverse (-)
87074879 .. 87080159
5281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG654000.1

Sequence Viewer

Length: 732 bp
ATGATGAAGACGTCATCGATGACGGAGCTCGATGACGAAACGGTGCGCAGCATGGCCATCGGCGCCGTCTTCTCGGACTTCGGTGGGAAGATAAACTCACTCGATTTTCATCGAAAGGAAGATTTACTTGTTACTGCCAGTGAGGATGACTCTGTGCGACTCTATGACATTGCAACTGCCAAGTTGCTAAAGACCACATATCATAAGAAACATGGAGCAGATCGCATATGTTTTACTCATCACCCAAGCTCTGTGATATGCTCTTCAAGATACAACTTGGATTCTACCGGAGAATCACTGCGGTATTTGTCCATGTATGATAATAGATGCCTTCGCTACTTCAAAGGGCATAAAGAGAGAATTGTTTCCCTCTGTATGTCTCCTATCAACGATAGCTTCATGTCTGGTTCATTGGATCACAGTGTCCGAATATGGGATCTTCGTGTAAATACATGCCAGGGAATTTTACATCTACGTGGTAGACCTACAGTGGCCTATGACCAACAAGGTCTTGTTTTCGCTGTAGCTATGGAAGGGGGTGCTATCAAGTTGTTTGATTCACGCTCCTATGACAAGGGCCCATTTGATACGTTTTTAGTTGGTGGAGATACAGCTGAAGTTTGCGATATCAAATTTAGCAGTGATGGTAAATCAATGCTCCTGACCACCACGAACAATAACATCTATGTTCTTGATGCCTATGCAGGAGAGAAGGTATACTTTCTTGTTTAG

Protein Analysis

243

Amino Acids

27.26

Weight (kDa)

6.23

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 22 - 222 1.1e-20 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 26 - 90 1.4e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 28 - 151 1.1e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 30 - 236 1.3e-26 WDR5 beta-propeller domain
WDR55 PF24796 30 - 152 1.2e-06 WDR55
Beta-prop_WDR3_2nd PF25172 31 - 151 7.3e-08 WDR3 second beta-propeller domain
Beta-prop_WDR75_1st PF23869 88 - 147 5.6e-06 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 98 - 233 1.7e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 99 - 160 3.5e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 109 - 239 4.1e-16 THOC3 beta-propeller domain
WD40 PF00400 109 - 146 1.6e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 111 - 177 8.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 113 - 240 2e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 114 - 237 1.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 123 - 233 1.3e-11 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14530
fragaria_vesca FvH4_6g51320 FvH4_6g51320
malus_domestica MD09G1028100.v1.1
prunus_persica Prupe.3G290600_v2.0.a1 Prupe.3G290600_v2.0.a1
pyrus_communis pycom111g02200
rosa_chinensis RchiOBHm_Chr2g0172941
rosa_laevigata RLG00000022144
rosa_multiflora Rmu_sc0029209.1_g000002 Rmu_sc0029210.1_g000001
rosa_roxburghii Rroxscaffold_2G00079400
rosa_rugosa Rorug02G0563800
rosa_samantha Rh2AG640600 Rh2BG654000 Rh2CG619700 Rh2DG667700
rosa_wichuraiana Rw2G052880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
Acc16I TGCGCA 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 481, 717
AciI CCGC 1 cut(s) 301
AclWI GGATC 2 cut(s) 423, 444
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 462, 632
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 2 cut(s) 11, 63
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 2 cut(s) 267, 343
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 5 cut(s) 28, 249, 396, 527, 614
AluI AGCT 5 cut(s) 28, 249, 396, 527, 614
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 2 cut(s) 423, 444
AoxI GGCC 3 cut(s) 54, 492, 577
ApaI GGGCCC 1 cut(s) 581
ApeKI GCWGC 1 cut(s) 48
ApoI RAATTY 2 cut(s) 462, 632
Asp700I GAANNNNTTC 1 cut(s) 364
AspLEI GCGC 2 cut(s) 48, 65
AspS9I GGNCC 2 cut(s) 577, 578
AsuHPI GGTGA 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 30, 581
BbsI GAAGAC 2 cut(s) 14, 61
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 65, 638
BceAI ACGGC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 486, 522
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 577, 578
BmiI GGNNCC 2 cut(s) 64, 579
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 317, 685
BpiI GAAGAC 2 cut(s) 14, 61
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bsa29I ATCGAT 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 11, 63
BsaJI CCNNGG 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 458
BseCI ATCGAT 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 3 cut(s) 56, 494, 579
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 384
BsnI GGCC 3 cut(s) 56, 494, 579
Bsp120I GGGCCC 1 cut(s) 577
Bsp1286I GDGCHC 2 cut(s) 30, 581
Bsp143I GATC 3 cut(s) 220, 415, 436
BspACI CCGC 1 cut(s) 301
BspANI GGCC 3 cut(s) 56, 494, 579
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 64, 579
BspPI GGATC 2 cut(s) 423, 444
BspQI GCTCTTC 1 cut(s) 268
BspT107I GGYRCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 3 cut(s) 220, 415, 436
BssNAI GTATAC 1 cut(s) 718
BssNI GRCGYC 2 cut(s) 11, 63
Bst1107I GTATAC 1 cut(s) 718
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 3 cut(s) 43, 422, 490
Bst6I CTCTTC 1 cut(s) 268
BstACI GRCGYC 2 cut(s) 11, 63
BstBAI YACGTR 1 cut(s) 476
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 48, 65
BstKTI GATC 3 cut(s) 223, 418, 439
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 3 cut(s) 220, 415, 436
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 2 cut(s) 486, 522
BstSLI GKGCMC 1 cut(s) 581
BstV1I GCAGC 1 cut(s) 60
BstV2I GAAGAC 2 cut(s) 14, 61
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 718
Bsu15I ATCGAT 1 cut(s) 17
BsuRI GGCC 3 cut(s) 56, 494, 579
BsuTUI ATCGAT 1 cut(s) 17
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 2 cut(s) 296, 646
BtsIMutI CAGTG 5 cut(s) 145, 296, 427, 495, 646
CfoI GCGC 2 cut(s) 48, 65
Cfr13I GGNCC 2 cut(s) 577, 578
ClaI ATCGAT 1 cut(s) 17
CviAII CATG 5 cut(s) 52, 212, 313, 400, 453
CviJI RGCY 8 cut(s) 28, 56, 249, 396, 494, 527, 579, 614
CviKI_1 RGCY 8 cut(s) 28, 56, 249, 396, 494, 527, 579, 614
DinI GGCGCC 1 cut(s) 64
DpnI GATC 3 cut(s) 222, 417, 438
DpnII GATC 3 cut(s) 220, 415, 436
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Ecl136II GAGCTC 1 cut(s) 28
Eco24I GRGCYC 2 cut(s) 30, 581
Eco32I GATATC 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 456
EcoRV GATATC 1 cut(s) 628
EcoT38I GRGCYC 2 cut(s) 30, 581
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaeI CATG 5 cut(s) 55, 215, 316, 403, 456
FalI AAGNNNNNCTT 3 cut(s) 111, 143, 704
FatI CATG 5 cut(s) 51, 211, 312, 399, 452
FauNDI CATATG 1 cut(s) 227
FblI GTMKAC 2 cut(s) 481, 717
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 2 cut(s) 30, 581
Fsp4HI GCNGC 1 cut(s) 49
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 47, 64
GluI GCNGC 1 cut(s) 49
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 3 cut(s) 56, 494, 579
HapII CCGG 1 cut(s) 288
HhaI GCGC 2 cut(s) 48, 65
Hin1I GRCGYC 2 cut(s) 11, 63
Hin1II CATG 5 cut(s) 55, 215, 316, 403, 456
Hin6I GCGC 2 cut(s) 46, 63
HinP1I GCGC 2 cut(s) 46, 63
HinfI GANTC 5 cut(s) 149, 159, 281, 293, 557
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 2 cut(s) 482, 718
Hpy188I TCNGA 2 cut(s) 76, 428
Hpy188III TCNNGA 3 cut(s) 267, 661, 692
Hpy8I GTNNAC 2 cut(s) 482, 718
HpyAV CCTTC 3 cut(s) 341, 527, 706
HpyCH4III ACNGT 3 cut(s) 43, 422, 490
HpyCH4IV ACGT 3 cut(s) 11, 475, 590
HpyCH4V TGCA 2 cut(s) 173, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 3 cut(s) 11, 475, 590
Hsp92I GRCGYC 2 cut(s) 11, 63
Hsp92II CATG 5 cut(s) 55, 215, 316, 403, 456
HspAI GCGC 2 cut(s) 46, 63
KasI GGCGCC 1 cut(s) 62
Kzo9I GATC 3 cut(s) 220, 415, 436
LguI GCTCTTC 1 cut(s) 268
LmnI GCTCC 4 cut(s) 25, 215, 569, 663
LpnPI CCDG 7 cut(s) 151, 301, 390, 443, 470, 674, 690
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 2 cut(s) 317, 685
MaeII ACGT 3 cut(s) 11, 475, 590
MaeIII GTNAC 1 cut(s) 130
MalI GATC 3 cut(s) 222, 417, 438
MboI GATC 3 cut(s) 220, 415, 436
MboII GAAGA 6 cut(s) 19, 61, 100, 131, 255, 431
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 30, 581
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 3 cut(s) 360, 462, 632
MluNI TGGCCA 1 cut(s) 56
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 2 cut(s) 143, 153
MnlI CCTC 2 cut(s) 136, 380
Mox20I TGGCCA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 364
MscI TGGCCA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 62
NarI GGCGCC 1 cut(s) 63
NdeI CATATG 1 cut(s) 227
NdeII GATC 3 cut(s) 220, 415, 436
NlaIII CATG 5 cut(s) 55, 215, 316, 403, 456
NlaIV GGNNCC 2 cut(s) 64, 579
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 364
PfeI GAWTC 3 cut(s) 281, 293, 557
PkrI GCNGC 1 cut(s) 50
PleI GAGTC 2 cut(s) 143, 153
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 2 cut(s) 143, 153
Ppu21I YACGTR 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 64, 579
PspOMI GGGCCC 1 cut(s) 577
PspPI GGNCC 2 cut(s) 577, 578
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 614
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 268
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 3 cut(s) 220, 415, 436
Sau96I GGNCC 2 cut(s) 577, 578
SchI GAGTC 2 cut(s) 143, 153
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 2 cut(s) 30, 581
SfaNI GCATC 2 cut(s) 317, 685
SfcI CTRYAG 2 cut(s) 486, 522
SfoI GGCGCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 3 cut(s) 360, 462, 632
SsiI CCGC 1 cut(s) 301
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 456
TaaI ACNGT 3 cut(s) 43, 422, 490
TaiI ACGT 3 cut(s) 14, 478, 593
TaqI TCGA 4 cut(s) 17, 30, 102, 112
TasI AATT 3 cut(s) 360, 462, 632
TfiI GAWTC 3 cut(s) 281, 293, 557
TscAI CASTG 5 cut(s) 145, 303, 427, 495, 646
TseI GCWGC 1 cut(s) 48
TspDTI ATGAA 4 cut(s) 20, 98, 388, 399
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 5 cut(s) 145, 303, 427, 495, 646
XapI RAATTY 2 cut(s) 462, 632
XceI RCATGY 1 cut(s) 456
XmiI GTMKAC 2 cut(s) 481, 717
XmnI GAANNNNTTC 1 cut(s) 364
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.