RLG00000022144

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013284
Physical Location & Seq
Reverse (-)
81307249 .. 81310312
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022144

Sequence Viewer

Length: 1029 bp
ATGATGAAGACGTCATCGATGACGGAGCTCGATGACGAAACGGTGAGCAGCATGGCCATCGGCGCCGTCTTCTCGGACTTCGGTGGGAAGATAAACTCACTCGATTTTCATCGGAAGGAAGATTTACTTGTTACTGCCAGTGAGGATGACTCTGTGCGACTCTATGACATTGCAACTGCCAAGTTGCTAAAGACCACATATCATAAGAAACATGGAGCAGATCGCATATGCTTTACTCATCACCCAAGCTCTGTGATATGCTCTTCAAGATACAATTTGGATTCTACTGGAGAATCACTGCGGTATTTGTCCATGTATGATAATAGATGCCTTCGCTACTTCAAAGGGCATAACGAGAGAATTGTTTCCCTCTGTATGTCTCCTATCAACGATAGCTTCATGTCTGGTTCATTGGATCACAGGGTCCGAATATGGGATCTTCGTGTAAATACATGCCAGGGAATTTTACATCTACGTGGTAGACCTACAGTGGCCTATGACCACCAAGGTCTTGTTTTCGCTGTAGCTATGGAAGGGGGTGCTATCAAGTTGTTTGATTCACGCTCCTATGACAAGGGCCCATTTGATACATTTTTAGTTGGTGGAGATACAGCTGAAGTTTGCGATATCAAATTTAGCAGCGATGGCAAATCAATGCTCCTGACCACCACGAACAATAACATCTATGTTCTTGATGCCTATGCAGGAGAGAAGCGATGTGGTTACAGCATGGAACCATCTCCAAATACGAGTATAGAGGCAACATTTACCCCAGATGGCCAGTATGTGTTGTCAGGCTCTGGAGATGGAAATTTGCATGCTTGGAGCATTAACAGGCGGAATGAGGTCGCAAGCTGGAACAGCCATATTGGAGTCCCATCATGTTTGAAGTGGGCTCCTCGCAGAGCTATGTTTGCTGCTGCGTCGACTGTTCTCACCTTTTGGATACCAAACGATTCAAACGCTGATCCCACTGTTATGGACACTGAGCTCACTGCTGGTGCTGAATCGCAACATCCTTCTCAATGA

Protein Analysis

343

Amino Acids

37.88

Weight (kDa)

5.83

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 22 - 222 2.6e-20 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 27 - 90 3e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 27 - 151 2.6e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 30 - 310 4e-36 WDR5 beta-propeller domain
WDR55 PF24796 30 - 152 2.9e-06 WDR55
Beta-prop_WDR3_2nd PF25172 31 - 309 5.3e-12 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 98 - 233 2e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 99 - 160 2.3e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 109 - 308 1.4e-22 THOC3 beta-propeller domain
WD40 PF00400 109 - 146 6.6e-07 WD domain, G-beta repeat
Beta-prop_EML PF23409 112 - 296 7.4e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 113 - 275 9.2e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 115 - 236 4.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 123 - 285 7e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 164 - 307 2.1e-08 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 164 - 288 2.1e-08 WDHD1 first WD40 domain
WDR55 PF24796 172 - 310 6.3e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14530
fragaria_vesca FvH4_6g51320 FvH4_6g51320
malus_domestica MD09G1028100.v1.1
prunus_persica Prupe.3G290600_v2.0.a1 Prupe.3G290600_v2.0.a1
pyrus_communis pycom111g02200
rosa_chinensis RchiOBHm_Chr2g0172941
rosa_laevigata RLG00000022144
rosa_multiflora Rmu_sc0029209.1_g000002 Rmu_sc0029210.1_g000001
rosa_roxburghii Rroxscaffold_2G00079400
rosa_rugosa Rorug02G0563800
rosa_samantha Rh2AG640600 Rh2BG654000 Rh2CG619700 Rh2DG667700
rosa_wichuraiana Rw2G052880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 481, 926
AciI CCGC 2 cut(s) 301, 838
AclWI GGATC 3 cut(s) 423, 444, 962
AcoI YGGCCR 2 cut(s) 54, 778
AcsI RAATTY 3 cut(s) 462, 632, 811
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 2 cut(s) 11, 63
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 4 cut(s) 267, 343, 889, 960
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 8 cut(s) 28, 249, 396, 527, 614, 855, 908, 991
AluI AGCT 8 cut(s) 28, 249, 396, 527, 614, 855, 908, 991
Alw21I GWGCWC 2 cut(s) 30, 993
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 3 cut(s) 423, 444, 962
AlwNI CAGNNNCTG 1 cut(s) 800
AoxI GGCC 4 cut(s) 54, 492, 577, 778
ApaI GGGCCC 1 cut(s) 581
ApeKI GCWGC 4 cut(s) 48, 639, 917, 920
ApoI RAATTY 3 cut(s) 462, 632, 811
Asp700I GAANNNNTTC 1 cut(s) 364
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 3 cut(s) 424, 577, 578
AsuHPI GGTGA 3 cut(s) 55, 233, 928
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 2 cut(s) 56, 780
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 4 cut(s) 30, 581, 898, 993
BbsI GAAGAC 2 cut(s) 14, 61
Bbv12I GWGCWC 2 cut(s) 30, 993
BbvI GCAGC 4 cut(s) 60, 651, 904, 907
BccI CCATC 6 cut(s) 65, 638, 745, 770, 800, 886
BceAI ACGGC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 939
BcoDI GTCTC 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 486, 522
BfoI RGCGCY 1 cut(s) 66
BfuI GTATCC 1 cut(s) 939
BisI GCNGC 4 cut(s) 49, 640, 918, 921
BlsI GCNGC 4 cut(s) 50, 641, 919, 922
Bme1390I CCNGG 1 cut(s) 458
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 3 cut(s) 424, 577, 578
BmiI GGNNCC 5 cut(s) 64, 425, 579, 735, 897
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 317, 685
BpiI GAAGAC 2 cut(s) 14, 61
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 2 cut(s) 309, 822
Bsa29I ATCGAT 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 11, 63
BsaJI CCNNGG 2 cut(s) 457, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 3 cut(s) 138, 292, 781
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 458
BseCI ATCGAT 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 457, 505
BseGI GGATG 2 cut(s) 151, 1015
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 3 cut(s) 138, 292, 781
BseRI GAGGAG 1 cut(s) 888
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 4 cut(s) 60, 651, 904, 907
BshFI GGCC 4 cut(s) 56, 494, 579, 780
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 2 cut(s) 30, 993
BslFI GGGAC 1 cut(s) 860
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 860
BsnI GGCC 4 cut(s) 56, 494, 579, 780
Bsp120I GGGCCC 1 cut(s) 577
Bsp1286I GDGCHC 4 cut(s) 30, 581, 898, 993
Bsp143I GATC 4 cut(s) 220, 415, 436, 967
BspACI CCGC 2 cut(s) 301, 838
BspANI GGCC 4 cut(s) 56, 494, 579, 780
BspCNI CTCAG 1 cut(s) 979
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 5 cut(s) 64, 425, 579, 735, 897
BspPI GGATC 3 cut(s) 423, 444, 962
BspQI GCTCTTC 1 cut(s) 268
BspT107I GGYRCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 3 cut(s) 138, 292, 781
BssECI CCNNGG 2 cut(s) 457, 505
BssMI GATC 4 cut(s) 220, 415, 436, 967
BssNI GRCGYC 2 cut(s) 11, 63
BssT1I CCWWGG 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 4 cut(s) 43, 490, 931, 976
Bst6I CTCTTC 1 cut(s) 268
BstACI GRCGYC 2 cut(s) 11, 63
BstBAI YACGTR 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 819, 853
BstDEI CTNAG 1 cut(s) 987
BstF5I GGATG 2 cut(s) 151, 1015
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 4 cut(s) 223, 418, 439, 970
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 4 cut(s) 220, 415, 436, 967
BstMWI GCNNNNNNNGC 4 cut(s) 62, 645, 861, 914
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 2 cut(s) 456, 821
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 2 cut(s) 486, 522
BstSLI GKGCMC 1 cut(s) 581
BstV1I GCAGC 4 cut(s) 60, 651, 904, 907
BstV2I GAAGAC 2 cut(s) 14, 61
BstX2I RGATCY 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 979
BstYI RGATCY 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 17
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 4 cut(s) 56, 494, 579, 780
BsuTUI ATCGAT 1 cut(s) 17
BtgZI GCGATG 2 cut(s) 657, 730
BtsCI GGATG 2 cut(s) 151, 1015
BtsI GCAGTG 2 cut(s) 296, 993
BtsIMutI CAGTG 6 cut(s) 145, 296, 495, 972, 984, 993
Cac8I GCNNGC 2 cut(s) 819, 853
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 424, 577, 578
ClaI ATCGAT 1 cut(s) 17
CseI GACGC 1 cut(s) 912
CviAII CATG 8 cut(s) 52, 212, 313, 400, 453, 730, 818, 882
DdeI CTNAG 1 cut(s) 987
DinI GGCGCC 1 cut(s) 64
DpnI GATC 4 cut(s) 222, 417, 438, 969
DpnII GATC 4 cut(s) 220, 415, 436, 967
EaeI YGGCCR 2 cut(s) 54, 778
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
EciI GGCGGA 1 cut(s) 853
Ecl136II GAGCTC 2 cut(s) 28, 991
Eco130I CCWWGG 1 cut(s) 505
Eco24I GRGCYC 4 cut(s) 30, 581, 898, 993
Eco32I GATATC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 2 cut(s) 28, 991
Eco57I CTGAAG 1 cut(s) 636
EcoICRI GAGCTC 2 cut(s) 28, 991
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 456
EcoRV GATATC 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 505
EcoT38I GRGCYC 4 cut(s) 30, 581, 898, 993
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 505
FaeI CATG 8 cut(s) 55, 215, 316, 403, 456, 733, 821, 885
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 1 cut(s) 860
FatI CATG 8 cut(s) 51, 211, 312, 399, 452, 729, 817, 881
FauNDI CATATG 1 cut(s) 227
FblI GTMKAC 2 cut(s) 481, 926
Fnu4HI GCNGC 4 cut(s) 49, 640, 918, 921
FokI GGATG 2 cut(s) 158, 1002
FriOI GRGCYC 4 cut(s) 30, 581, 898, 993
Fsp4HI GCNGC 4 cut(s) 49, 640, 918, 921
GlaI GCGC 1 cut(s) 64
GluI GCNGC 4 cut(s) 49, 640, 918, 921
GsuI CTGGAG 2 cut(s) 309, 822
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 56, 494, 579, 780
HgaI GACGC 1 cut(s) 912
HhaI GCGC 1 cut(s) 65
Hin1I GRCGYC 2 cut(s) 11, 63
Hin1II CATG 8 cut(s) 55, 215, 316, 403, 456, 733, 821, 885
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 8 cut(s) 149, 159, 281, 293, 557, 873, 956, 1007
HphI GGTGA 3 cut(s) 55, 233, 928
Hpy166II GTNNAC 2 cut(s) 482, 927
Hpy188I TCNGA 3 cut(s) 76, 114, 428
Hpy188III TCNNGA 4 cut(s) 267, 661, 692, 801
Hpy8I GTNNAC 2 cut(s) 482, 927
Hpy99I CGWCG 1 cut(s) 928
HpyAV CCTTC 4 cut(s) 109, 341, 527, 1029
HpyCH4III ACNGT 4 cut(s) 43, 490, 931, 976
HpyCH4IV ACGT 2 cut(s) 11, 475
HpyCH4V TGCA 3 cut(s) 173, 704, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 645, 861, 914
HpyF3I CTNAG 1 cut(s) 987
HpySE526I ACGT 2 cut(s) 11, 475
Hsp92I GRCGYC 2 cut(s) 11, 63
Hsp92II CATG 8 cut(s) 55, 215, 316, 403, 456, 733, 821, 885
HspAI GCGC 1 cut(s) 63
KasI GGCGCC 1 cut(s) 62
Kzo9I GATC 4 cut(s) 220, 415, 436, 967
LguI GCTCTTC 1 cut(s) 268
LmnI GCTCC 6 cut(s) 25, 215, 569, 663, 825, 901
Lsp1109I GCAGC 4 cut(s) 60, 651, 904, 907
LweI GCATC 2 cut(s) 317, 685
MaeII ACGT 2 cut(s) 11, 475
MaeIII GTNAC 2 cut(s) 130, 722
MalI GATC 4 cut(s) 222, 417, 438, 969
MboI GATC 4 cut(s) 220, 415, 436, 967
MboII GAAGA 6 cut(s) 19, 61, 100, 131, 255, 431
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 4 cut(s) 30, 581, 898, 993
MlsI TGGCCA 2 cut(s) 56, 780
MluCI AATT 5 cut(s) 274, 360, 462, 632, 811
MluNI TGGCCA 2 cut(s) 56, 780
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 143, 153, 882
MnlI CCTC 5 cut(s) 136, 380, 751, 838, 909
Mox20I TGGCCA 2 cut(s) 56, 780
MroXI GAANNNNTTC 1 cut(s) 364
MscI TGGCCA 2 cut(s) 56, 780
MseI TTAA 1 cut(s) 831
MslI CAYNNNNRTG 2 cut(s) 474, 977
Msp20I TGGCCA 2 cut(s) 56, 780
MspA1I CMGCKG 1 cut(s) 614
MspR9I CCNGG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 4 cut(s) 62, 645, 861, 914
NarI GGCGCC 1 cut(s) 63
NdeI CATATG 1 cut(s) 227
NdeII GATC 4 cut(s) 220, 415, 436, 967
NlaIII CATG 8 cut(s) 55, 215, 316, 403, 456, 733, 821, 885
NlaIV GGNNCC 5 cut(s) 64, 425, 579, 735, 897
NspI RCATGY 2 cut(s) 456, 821
PaeI GCATGC 1 cut(s) 821
PciSI GCTCTTC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 364
PfeI GAWTC 5 cut(s) 281, 293, 557, 956, 1007
PkrI GCNGC 4 cut(s) 50, 641, 919, 922
PleI GAGTC 3 cut(s) 143, 153, 881
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 143, 153, 881
Ppu21I YACGTR 1 cut(s) 476
Psp124BI GAGCTC 2 cut(s) 30, 993
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 5 cut(s) 64, 425, 579, 735, 897
PspOMI GGGCCC 1 cut(s) 577
PspPI GGNCC 3 cut(s) 424, 577, 578
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 614
RseI CAYNNNNRTG 2 cut(s) 474, 977
SacI GAGCTC 2 cut(s) 30, 993
SalI GTCGAC 1 cut(s) 925
SapI GCTCTTC 1 cut(s) 268
SaqAI TTAA 1 cut(s) 831
SatI GCNGC 4 cut(s) 49, 640, 918, 921
Sau3AI GATC 4 cut(s) 220, 415, 436, 967
Sau96I GGNCC 3 cut(s) 424, 577, 578
SchI GAGTC 3 cut(s) 143, 153, 882
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 4 cut(s) 30, 581, 898, 993
SfaNI GCATC 2 cut(s) 317, 685
SfcI CTRYAG 2 cut(s) 486, 522
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 2 cut(s) 474, 977
SphI GCATGC 1 cut(s) 821
Sse9I AATT 5 cut(s) 274, 360, 462, 632, 811
SsiI CCGC 2 cut(s) 301, 838
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 2 cut(s) 30, 993
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 505
TaaI ACNGT 4 cut(s) 43, 490, 931, 976
TaiI ACGT 2 cut(s) 14, 478
TaqI TCGA 4 cut(s) 17, 30, 102, 926
TasI AATT 5 cut(s) 274, 360, 462, 632, 811
TfiI GAWTC 5 cut(s) 281, 293, 557, 956, 1007
Tru1I TTAA 1 cut(s) 831
Tru9I TTAA 1 cut(s) 831
TscAI CASTG 6 cut(s) 145, 303, 495, 979, 991, 1000
TseI GCWGC 4 cut(s) 48, 639, 917, 920
TspDTI ATGAA 4 cut(s) 20, 98, 388, 399
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 6 cut(s) 145, 303, 495, 979, 991, 1000
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 3 cut(s) 462, 632, 811
XceI RCATGY 2 cut(s) 456, 821
XmiI GTMKAC 2 cut(s) 481, 926
XmnI GAANNNNTTC 1 cut(s) 364
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.