pycom111g02230

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015439
Physical Location & Seq
Forward (+)
1337585 .. 1339510
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02230.1

Sequence Viewer

Length: 1926 bp
ATGAGTCGCGCTTCAACTCGTTCCGTAACTCGGACCTGGGTTTTACCGACGAACTCTCGCAGTCGCAGAAAACCATTTCCCCCCATTAATTTCTCACCAAAAATCTCAGCTTTCTCTTCTTCTTCTTCTTCTTCTTCGTCAGCACTGCAGACAATCCCAGACTCTCCCTCCAAACCAATCTCCAACCCACTTTACCATTTTCTCCCCCAAACCCAAAACCCTAACAACACAGTCGATCTCATCTGCTCCTCCCTCAAGCAAGGAAAACCCCATCTTGCCCTCCTCCAAAACGACATCAAAGGCCTTCTTCCCCACCTGGGTGCGCGGGAAATTTCGAGGGTTTTACTCAGGTTTCAATCCGATCACTCCTCGGCTATTGCTTTTTTCAACTGGGTCAAAAATGATTTGGGTCTCAGACCCTCCACCCAGAATTACTGCATTGTTGTTCATATTCTTGCGTGCTGTAAAAAGTTCCCGAAAGCCATGAATTTGTTGAGGGAGTTGATAGTGTTGTTGGGTAGAGATGTTTCACCAAAAGATGACATTTTTCGGAGTTTGGTTTCGTGCACCGAAGATTGCAACTGGGATCCGGTTGTTTTCGATATGCTCATCAAGGCTTATGTGAAAGCAGGTATGATCAGAGATGGTTTAAGCACTTTTAGAAAGATTGTAGATGTTGGTTTTGTTCCGAGTGTGGTTTCTTGTAATTGTCTTTTGAATGCGTTGGTGAAGTTGAATCGAATTGATCAGTGTTGGGATGTGTATGAGGAGATGGGAAGGATTGGGATACACCCAAATGTGTATACGTTTAATATTTTGATCAATGTTTTGTGTAAAGATGGAGATGTGGAGAAAGTGAATGCGTTTATGGAGAAGATTGAAGAAGAAGGATTTGATCCGGATATCGTGACGTATAACACGCTGATTAGTAGCTATTGTCGAAAAGGAAGGATGGAAGATGCATTTTATTTGTATAAGATCATGTATAGGAGAGGTGTGGTGCCGGATATGGTTTCGTACACTGCCTTGATGAATGGTCTTTGTAAGCAAGGGAAGGTAAGGGAGGCTCATCAGATTTTTCATCGGATGGTCGATAGAGGGTTGGATCAAGACGCTGTGACGTATAACACTCTTATTAGTGGTTATTGCCGCGAGGGGAAGATGCAAGAGTGCAGATTACTGTTGCACGAGATGATCAGAAAGGGAATTTGTCCAGATGATTTCACTTGTCGGGTAGTCGTAGAAGGATATGGCAAAGAAGGTAAACTGCTCTCTGCTCTGAATTTGGTCGTCGAGCTTCAAAAATTTCAGATATCGATTTCTCATGGTATTTACGATTATCTAATAGTTGCATTGTGTCGAGAAAATCATCCGTTTGCAGCTAAAAGTCTATTAGAAAGAAGTTTCCAGGATGGTTATCTTCCTGGTTTGGATACCTATAACAATCTAATCGAGTCTCTCTGCGAAAGCTACCACGTTACAGAGGCATTGCTTTTGAAAGCCGAGATGGTATGTAAGAACATTAAACCCAATCTTACGACATACAGAACTCTAATTTGCTGTCTCTGCCAAACAAACAGAACAGCAGAAGGTGAATCGCTAATGGAAGAGATGACTGAATCCGGTTTGGCTCCTGATCTCGAAATATGCAGGGCGTTGATAAAGGGGTACTGCAACGAAAGAGATGTCGATAAAGCGGAAGCTTTGTTAGGATTCTTTGCCAAAGAATTTCAAGTTTTTGACAGTGAAAGTTACAATGCACTTGTCCAGGTTTTGTGTGAGGATGGTGATATGGCTAAGTTGCTGGAGTTGCAGGAGAGGATGATGAAACTAGGGTTTGCGCCGAACAGCCTTACGTTTAAGTATGTGATCCATGGATTGTGGAAAACTGCAACGCCGGATAAAGAAAAACTTTGTGTGTTATAA

Protein Analysis

642

Amino Acids

72.57

Weight (kDa)

7.41

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 192 - 285 5.7e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 230 - 279 2.5e-17 PPR repeat family
PPR_long PF17177 245 - 364 3e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 254 - 312 7.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 262 - 285 1.4e-08 PPR repeat
PPR_2 PF13041 269 - 310 2.5e-10 PPR repeat family
PPR_1 PF12854 297 - 328 8.8e-13 PPR repeat
PPR_2 PF13041 300 - 349 1.8e-20 PPR repeat family
PPR PF01535 303 - 333 4.4e-09 PPR repeat
PPR_3 PF13812 328 - 382 1.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 331 - 364 6e-11 PPR repeat
PPR_2 PF13041 335 - 375 7.2e-12 PPR repeat family
PPR PF01535 338 - 368 1e-08 PPR repeat
PPR_1 PF12854 366 - 398 1e-09 PPR repeat
PPR_2 PF13041 371 - 418 1.9e-14 PPR repeat family
PPR PF01535 373 - 403 1.1e-10 PPR repeat
PPR_2 PF13041 478 - 524 1.9e-11 PPR repeat family
PPR_1 PF12854 506 - 539 1.2e-06 PPR repeat
PPR_2 PF13041 582 - 627 4.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015439)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40400 AT5G40400
fragaria_vesca FvH4_5g31860
malus_domestica MD09G1028300.v1.1
prunus_persica Prupe.3G290200_v2.0.a1
pyrus_communis pycom111g02230
rosa_chinensis RchiOBHm_Chr2g0173011
rosa_laevigata RLG00000022149
rosa_multiflora Rmu_sc0012743.1_g000003
rosa_roxburghii Rroxscaffold_2G00079320
rosa_samantha Rh2AG641500 Rh2AG644000 Rh2BG654800 Rh2CG620200 Rh2DG668400
rosa_wichuraiana Rw2G052920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1924
Acc36I ACCTGC 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 1000
AccI GTMKAC 1 cut(s) 803
AccII CGCG 3 cut(s) 9, 325, 1152
AccIII TCCGGA 1 cut(s) 898
AciI CCGC 3 cut(s) 325, 1150, 1697
AclWI GGATC 5 cut(s) 581, 594, 890, 1113, 1864
AcsI RAATTY 6 cut(s) 330, 487, 1206, 1282, 1304, 1727
AfaI GTAC 2 cut(s) 1019, 1670
AfiI CCNNNNNNNGG 2 cut(s) 30, 317
AgsI TTSAA 9 cut(s) 15, 356, 388, 718, 736, 881, 1301, 1498, 1733
AjnI CCWGG 5 cut(s) 35, 315, 1407, 1423, 1767
AleI CACNNNNGTG 1 cut(s) 318
AluBI AGCT 6 cut(s) 110, 933, 1297, 1382, 1470, 1703
AluI AGCT 6 cut(s) 110, 933, 1297, 1382, 1470, 1703
Alw21I GWGCWC 1 cut(s) 569
Alw26I GTCTC 3 cut(s) 416, 1461, 1568
Alw44I GTGCAC 1 cut(s) 565
AlwI GGATC 5 cut(s) 581, 594, 890, 1113, 1864
Aor13HI TCCGGA 1 cut(s) 898
AoxI GGCC 1 cut(s) 301
ApaLI GTGCAC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 1379
ApoI RAATTY 6 cut(s) 330, 487, 1206, 1282, 1304, 1727
ArsI GACNNNNNNTTYG 2 cut(s) 1671, 1703
AseI ATTAAT 1 cut(s) 87
AspLEI GCGC 3 cut(s) 11, 325, 1843
AspS9I GGNCC 1 cut(s) 33
AsuHPI GGTGA 5 cut(s) 87, 522, 739, 1604, 1799
AvaII GGWCC 1 cut(s) 33
BaeGI GKGCMC 1 cut(s) 569
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 1000
BauI CACGAG 1 cut(s) 1187
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 1 cut(s) 1391
BccI CCATC 9 cut(s) 279, 638, 766, 833, 946, 1081, 1406, 1501, 1778
BciT130I CCWGG 5 cut(s) 37, 317, 1409, 1425, 1769
BciVI GTATCC 2 cut(s) 780, 1426
BclI TGATCA 4 cut(s) 636, 745, 819, 1194
BcoDI GTCTC 3 cut(s) 416, 1461, 1568
BfaI CTAG 1 cut(s) 1832
BfmI CTRYAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 620
BfuI GTATCC 2 cut(s) 780, 1426
BisI GCNGC 2 cut(s) 1150, 1380
BlsI GCNGC 2 cut(s) 1151, 1381
Bme1390I CCNGG 5 cut(s) 37, 317, 1409, 1425, 1769
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 3 cut(s) 588, 1002, 1632
BmrFI CCNGG 5 cut(s) 37, 317, 1409, 1425, 1769
BmrI ACTGGG 2 cut(s) 400, 592
BmsI GCATC 2 cut(s) 949, 1152
BmuI ACTGGG 2 cut(s) 400, 592
BpmI CTGGAG 1 cut(s) 1826
BpuEI CTTGAG 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 1316
BsaBI GATNNNNATC 1 cut(s) 1416
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 4 cut(s) 36, 316, 369, 1873
BsaWI WCCGGW 3 cut(s) 589, 898, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 317
Bse1I ACTGG 2 cut(s) 395, 587
Bse3DI GCAATG 1 cut(s) 1487
Bse8I GATNNNNATC 1 cut(s) 1416
BseAI TCCGGA 1 cut(s) 898
BseBI CCWGG 5 cut(s) 37, 317, 1409, 1425, 1769
BseCI ATCGAT 1 cut(s) 1316
BseDI CCNNGG 4 cut(s) 36, 316, 369, 1873
BseGI GGATG 7 cut(s) 763, 957, 1092, 1369, 1417, 1789, 1827
BseJI GATNNNNATC 1 cut(s) 1416
BseLI CCNNNNNNNGG 2 cut(s) 30, 317
BseMI GCAATG 1 cut(s) 1487
BseMII CTCAG 3 cut(s) 120, 361, 427
BseNI ACTGG 2 cut(s) 395, 587
BseRI GAGGAG 4 cut(s) 238, 272, 358, 782
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 1 cut(s) 1391
BsgI GTGCAG 1 cut(s) 1192
Bsh1236I CGCG 3 cut(s) 9, 325, 1152
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 1000
BshVI ATCGAT 1 cut(s) 1316
BsiHKAI GWGCWC 1 cut(s) 569
BsiSI CCGG 5 cut(s) 590, 899, 1004, 1623, 1898
BslI CCNNNNNNNGG 2 cut(s) 30, 317
BsmAI GTCTC 3 cut(s) 416, 1461, 1568
BsmI GAATGC 2 cut(s) 724, 865
BsnI GGCC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 569
Bsp13I TCCGGA 1 cut(s) 898
Bsp19I CCATGG 1 cut(s) 1873
BspACI CCGC 3 cut(s) 325, 1150, 1697
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 3 cut(s) 119, 360, 426
BspDI ATCGAT 1 cut(s) 1316
BspEI TCCGGA 1 cut(s) 898
BspFNI CGCG 3 cut(s) 9, 325, 1152
BspLI GGNNCC 3 cut(s) 588, 1002, 1632
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 620
BspPI GGATC 5 cut(s) 581, 594, 890, 1113, 1864
BspT107I GGYRCC 1 cut(s) 1000
BspTNI GGTCTC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 1487
BsrI ACTGG 2 cut(s) 395, 587
BssECI CCNNGG 4 cut(s) 36, 316, 369, 1873
BssNAI GTATAC 1 cut(s) 804
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 1 cut(s) 1873
Bst1107I GTATAC 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 5 cut(s) 37, 317, 1409, 1425, 1769
Bst4CI ACNGT 3 cut(s) 232, 1182, 1745
Bst6I CTCTTC 2 cut(s) 121, 1602
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 4 cut(s) 106, 347, 413, 1797
BstDSI CCRYGG 1 cut(s) 1873
BstF5I GGATG 7 cut(s) 763, 957, 1092, 1369, 1417, 1789, 1827
BstFNI CGCG 3 cut(s) 9, 325, 1152
BstHHI GCGC 3 cut(s) 11, 325, 1843
BstMAI GTCTC 3 cut(s) 416, 1461, 1568
BstMWI GCNNNNNNNGC 2 cut(s) 1566, 1810
BstNI CCWGG 5 cut(s) 37, 317, 1409, 1425, 1769
BstSCI CCNGG 5 cut(s) 35, 315, 1407, 1423, 1767
BstSFI CTRYAG 1 cut(s) 146
BstSLI GKGCMC 1 cut(s) 569
BstUI CGCG 3 cut(s) 9, 325, 1152
BstV1I GCAGC 1 cut(s) 1391
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BstZ17I GTATAC 1 cut(s) 804
Bsu15I ATCGAT 1 cut(s) 1316
BsuI GTATCC 2 cut(s) 780, 1426
BsuRI GGCC 1 cut(s) 303
BsuTUI ATCGAT 1 cut(s) 1316
BtgI CCRYGG 1 cut(s) 1873
BtsCI GGATG 7 cut(s) 763, 957, 1092, 1369, 1417, 1789, 1827
BtsI GCAGTG 2 cut(s) 143, 1020
BtsIMutI CAGTG 4 cut(s) 143, 755, 1020, 1750
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 1 cut(s) 460
CfoI GCGC 3 cut(s) 11, 325, 1843
Cfr13I GGNCC 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 1316
CseI GACGC 1 cut(s) 1121
Csp6I GTAC 2 cut(s) 1018, 1669
CviAII CATG 4 cut(s) 484, 982, 1325, 1874
CviQI GTAC 2 cut(s) 1018, 1669
DdeI CTNAG 4 cut(s) 106, 347, 413, 1797
Eam1104I CTCTTC 2 cut(s) 121, 1602
EarI CTCTTC 2 cut(s) 121, 1602
Eco130I CCWWGG 1 cut(s) 1873
Eco147I AGGCCT 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 416
Eco32I GATATC 2 cut(s) 904, 1314
Eco47I GGWCC 1 cut(s) 33
EcoRII CCWGG 5 cut(s) 35, 315, 1407, 1423, 1767
EcoRV GATATC 2 cut(s) 904, 1314
EcoT14I CCWWGG 1 cut(s) 1873
EcoT22I ATGCAT 1 cut(s) 964
ErhI CCWWGG 1 cut(s) 1873
FaeI CATG 4 cut(s) 487, 985, 1328, 1877
FalI AAGNNNNNCTT 3 cut(s) 291, 323, 1896
FatI CATG 4 cut(s) 483, 981, 1324, 1873
FauI CCCGC 1 cut(s) 318
FbaI TGATCA 4 cut(s) 636, 745, 819, 1194
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 2 cut(s) 1150, 1380
FokI GGATG 7 cut(s) 770, 964, 1099, 1356, 1424, 1796, 1834
Fsp4HI GCNGC 2 cut(s) 1150, 1380
FspBI CTAG 1 cut(s) 1832
GlaI GCGC 3 cut(s) 10, 324, 1842
GluI GCNGC 2 cut(s) 1150, 1380
GsuI CTGGAG 1 cut(s) 1826
HaeIII GGCC 1 cut(s) 303
HapII CCGG 5 cut(s) 590, 899, 1004, 1623, 1898
HgaI GACGC 1 cut(s) 1121
HhaI GCGC 3 cut(s) 11, 325, 1843
Hin1II CATG 4 cut(s) 487, 985, 1328, 1877
Hin6I GCGC 3 cut(s) 9, 323, 1841
HinP1I GCGC 3 cut(s) 9, 323, 1841
HindIII AAGCTT 1 cut(s) 1701
HinfI GANTC 7 cut(s) 4, 161, 736, 1454, 1595, 1619, 1713
HpaII CCGG 5 cut(s) 590, 899, 1004, 1623, 1898
HphI GGTGA 5 cut(s) 87, 522, 739, 1604, 1799
Hpy166II GTNNAC 4 cut(s) 567, 804, 1020, 1265
Hpy188III TCNNGA 8 cut(s) 475, 899, 907, 1109, 1214, 1361, 1634, 1640
Hpy8I GTNNAC 4 cut(s) 567, 804, 1020, 1265
Hpy99I CGWCG 2 cut(s) 52, 1295
HpyAV CCTTC 8 cut(s) 314, 771, 881, 942, 1048, 1238, 1253, 1583
HpyCH4III ACNGT 3 cut(s) 232, 1182, 1745
HpyCH4IV ACGT 5 cut(s) 806, 911, 1121, 1476, 1856
HpyF10VI GCNNNNNNNGC 2 cut(s) 1566, 1810
HpyF3I CTNAG 4 cut(s) 106, 347, 413, 1797
HpySE526I ACGT 5 cut(s) 806, 911, 1121, 1476, 1856
Hsp92II CATG 4 cut(s) 487, 985, 1328, 1877
HspAI GCGC 3 cut(s) 9, 323, 1841
Kpn2I TCCGGA 1 cut(s) 898
Ksp22I TGATCA 4 cut(s) 636, 745, 819, 1194
LmnI GCTCC 2 cut(s) 251, 1636
Lsp1109I GCAGC 1 cut(s) 1391
LweI GCATC 2 cut(s) 949, 1152
MaeI CTAG 1 cut(s) 1832
MaeII ACGT 5 cut(s) 806, 911, 1121, 1476, 1856
MaeIII GTNAC 5 cut(s) 25, 907, 1117, 1477, 1751
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 569
MlyI GAGTC 3 cut(s) 13, 155, 1463
MmeI TCCRAC 2 cut(s) 207, 1083
Mph1103I ATGCAT 1 cut(s) 964
MroI TCCGGA 1 cut(s) 898
MseI TTAA 5 cut(s) 87, 650, 810, 1524, 1860
MslI CAYNNNNRTG 2 cut(s) 318, 795
MspI CCGG 5 cut(s) 590, 899, 1004, 1623, 1898
MspR9I CCNGG 5 cut(s) 37, 317, 1409, 1425, 1769
Mva1269I GAATGC 2 cut(s) 724, 865
MvaI CCWGG 5 cut(s) 37, 317, 1409, 1425, 1769
MvnI CGCG 3 cut(s) 9, 325, 1152
MwoI GCNNNNNNNGC 2 cut(s) 1566, 1810
NcoI CCATGG 1 cut(s) 1873
NlaIII CATG 4 cut(s) 487, 985, 1328, 1877
NlaIV GGNNCC 3 cut(s) 588, 1002, 1632
NmeAIII GCCGAG 2 cut(s) 350, 1528
NmuCI GTSAC 2 cut(s) 907, 1117
NsiI ATGCAT 1 cut(s) 964
OliI CACNNNNGTG 1 cut(s) 318
PceI AGGCCT 1 cut(s) 303
PctI GAATGC 2 cut(s) 724, 865
PfeI GAWTC 4 cut(s) 736, 1595, 1619, 1713
PfoI TCCNGGA 1 cut(s) 1407
PkrI GCNGC 2 cut(s) 1151, 1381
PleI GAGTC 3 cut(s) 12, 155, 1462
PpsI GAGTC 3 cut(s) 12, 155, 1462
PshBI ATTAAT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 1924
Psp6I CCWGG 5 cut(s) 35, 315, 1407, 1423, 1767
PspGI CCWGG 5 cut(s) 35, 315, 1407, 1423, 1767
PspN4I GGNNCC 3 cut(s) 588, 1002, 1632
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 2 cut(s) 1019, 1670
RsaNI GTAC 2 cut(s) 1018, 1669
RseI CAYNNNNRTG 2 cut(s) 318, 795
SaqAI TTAA 5 cut(s) 87, 650, 810, 1524, 1860
SatI GCNGC 2 cut(s) 1150, 1380
Sau96I GGNCC 1 cut(s) 33
SchI GAGTC 3 cut(s) 13, 155, 1463
ScrFI CCNGG 5 cut(s) 37, 317, 1409, 1425, 1769
SduI GDGCHC 1 cut(s) 569
SfaNI GCATC 2 cut(s) 949, 1152
SfcI CTRYAG 1 cut(s) 146
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 2 cut(s) 318, 795
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SseBI AGGCCT 1 cut(s) 303
SsiI CCGC 3 cut(s) 325, 1150, 1697
SspI AATATT 1 cut(s) 814
SspMI CTAG 1 cut(s) 1832
StuI AGGCCT 1 cut(s) 303
StyD4I CCNGG 5 cut(s) 35, 315, 1407, 1423, 1767
StyI CCWWGG 1 cut(s) 1873
TaaI ACNGT 3 cut(s) 232, 1182, 1745
TaiI ACGT 5 cut(s) 809, 914, 1124, 1479, 1859
TauI GCSGC 1 cut(s) 1152
TfiI GAWTC 4 cut(s) 736, 1595, 1619, 1713
Tru1I TTAA 5 cut(s) 87, 650, 810, 1524, 1860
Tru9I TTAA 5 cut(s) 87, 650, 810, 1524, 1860
TscAI CASTG 4 cut(s) 150, 755, 1027, 1750
TseFI GTSAC 2 cut(s) 907, 1117
TseI GCWGC 1 cut(s) 1379
Tsp45I GTSAC 2 cut(s) 907, 1117
TspDTI ATGAA 5 cut(s) 437, 500, 1046, 1070, 1841
TspGWI ACGGA 2 cut(s) 13, 1362
TspRI CASTG 4 cut(s) 150, 755, 1027, 1750
VneI GTGCAC 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 33
VspI ATTAAT 1 cut(s) 87
XapI RAATTY 6 cut(s) 330, 487, 1206, 1282, 1304, 1727
XmiI GTMKAC 1 cut(s) 803
XspI CTAG 1 cut(s) 1832
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.