pycom111g03220

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0016906
Physical Location & Seq
Reverse (-)
1941118 .. 1943069
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03220.2

Sequence Viewer

Length: 1020 bp
ATGAGTTCGGGTTCAATATCCTGCTCTCCTGTCGTCACCAAAGACTTGGCCAAGAAGAAGAGGGTAAGCTCGCTGGCTATGTCGTCCATTCTCCTCCGCTCTCTGTCTTTTCTCTTTCGTGCATACGTCTGCACGAACAGGTTGGCGAAATTGAAGCAGAGCAAGCTCGATGTTCGCCGCGAGCAATGGCTTTCACATGTCAAGAATAAAGGGCACAAAGTGAACTCGGATGGGAGGGGTGGGTCTCCTACACCATCTAGGCAAGTAGAGAAAGGGCAAAATGGATCATTGGACAATTTGGAAACGAGTTCAACCGGAGGAGAGAATGATAGTTCGAGCATCCATGACAGTGATTTGGAGTCTCTGACAACCAGTCCGAGTGGAAGCAGTTTGGATTGTAATGATTCGATGAAGAATCTTAGTGAGAGCAGTGGAAGTGATTGTTTGGGAAATGTCAGCAAGGAGGATGCTGATGATGGGTGTCTAGATGACTGGGAGGCTGTGGCCGATGCTTTAAATGCAGAGGACAACAAGCATACCCCTATTATTGATTTTCCTTCCAAAGCTGAAATCACAGTTGAACCCACAGGCCTTGAACCATCTAGCGAGAATCCTGGAGTTGAATTATCGAATCCAGAGAACAGAAACATGGTTCCTGGGTCACGTACGGCGCGTTGTGCTTGGAGACCTGATGATGCTTCCCGTCCTCAGAATCTGCCCAATGTGCCAAAACAGCATAGTTTCGTGGTTCAATCAGACTGGCATTGTGGCCATGGAGCCATTGCATGGGCATGGCAAAACGTCGTGCCCCAGCCTTCTTCATGTCCTATATGCTATGAGGATTTAGATGTCACAGACTCGAGCTTTATTCCATGTTCATGCGGCTTTCGGCTTTGCCTCTTTTGCCACAAAAGGATTCTAGAGGCAGACGGGCGCTGTCCAGGCTGCAGGAAGCAGTACGATCATGGAAATGGAGCAATGAGCATCAACAGAGGAGCTACAACTTTTAGAGTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_4

Protein Analysis

340

Amino Acids

36.76

Weight (kDa)

5.87

Isoelectric Point (pI)

60.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016906)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49800 FvH4_6g49800
malus_domestica MD09G1041200.v1.1
prunus_persica Prupe.3G276400_v2.0.a1
pyrus_communis pycom111g03220
rosa_chinensis RchiOBHm_Chr2g0169941
rosa_laevigata RLG00000021912
rosa_roxburghii Rroxscaffold_2G00081710
rosa_rugosa Rorug02G0544500
rosa_samantha Rh2AG616600 Rh2BG628400 Rh2CG597700 Rh2DG639400
rosa_wichuraiana Rw2G051070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 786
AccBSI CCGCTC 1 cut(s) 99
AccII CGCG 2 cut(s) 180, 673
AciI CCGC 3 cut(s) 97, 178, 882
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 3 cut(s) 48, 504, 769
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 2 cut(s) 667, 959
AfiI CCNNNNNNNGG 1 cut(s) 786
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 7 cut(s) 15, 154, 312, 581, 596, 623, 752
AhdI GACNNNNNGTC 1 cut(s) 372
AjnI CCWGG 3 cut(s) 613, 655, 940
AluBI AGCT 5 cut(s) 69, 166, 566, 864, 998
AluI AGCT 5 cut(s) 69, 166, 566, 864, 998
Alw26I GTCTC 3 cut(s) 249, 366, 679
AlwI GGATC 1 cut(s) 292
AlwNI CAGNNNCTG 1 cut(s) 715
Ama87I CYCGRG 1 cut(s) 859
AoxI GGCC 4 cut(s) 48, 504, 589, 769
ApeKI GCWGC 1 cut(s) 945
AspLEI GCGC 2 cut(s) 673, 936
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 859
BaeGI GKGCMC 2 cut(s) 216, 810
BalI TGGCCA 2 cut(s) 50, 771
BbvI GCAGC 1 cut(s) 932
BccI CCATC 4 cut(s) 224, 262, 470, 607
BceAI ACGGC 1 cut(s) 684
BciT130I CCWGG 3 cut(s) 615, 657, 942
BcoDI GTCTC 3 cut(s) 249, 366, 679
BfaI CTAG 4 cut(s) 258, 485, 603, 920
BfmI CTRYAG 1 cut(s) 946
BfoI RGCGCY 1 cut(s) 937
BisI GCNGC 3 cut(s) 178, 883, 946
BlsI GCNGC 3 cut(s) 179, 884, 947
Bme1390I CCNGG 3 cut(s) 615, 657, 942
BmeRI GACNNNNNGTC 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 654, 778
BmrFI CCNGG 3 cut(s) 615, 657, 942
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 5 cut(s) 348, 457, 499, 685, 993
BmuI ACTGGG 1 cut(s) 502
BpmI CTGGAG 1 cut(s) 636
BsaAI YACGTR 1 cut(s) 665
BsaI GGTCTC 2 cut(s) 249, 679
BsaJI CCNNGG 2 cut(s) 656, 772
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 1 cut(s) 786
Bse1I ACTGG 3 cut(s) 372, 497, 764
Bse3DI GCAATG 3 cut(s) 191, 780, 984
BseBI CCWGG 3 cut(s) 615, 657, 942
BseDI CCNNGG 2 cut(s) 656, 772
BseGI GGATG 3 cut(s) 235, 339, 472
BseLI CCNNNNNNNGG 1 cut(s) 786
BseMI GCAATG 3 cut(s) 191, 780, 984
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 3 cut(s) 372, 497, 764
BseRI GAGGAG 3 cut(s) 83, 333, 1008
BseSI GKGCMC 2 cut(s) 216, 810
BseXI GCAGC 1 cut(s) 932
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 2 cut(s) 180, 673
BshFI GGCC 4 cut(s) 50, 506, 591, 771
BsiHKCI CYCGRG 1 cut(s) 859
BsiSI CCGG 1 cut(s) 315
BsiWI CGTACG 1 cut(s) 665
BslI CCNNNNNNNGG 1 cut(s) 786
BsmAI GTCTC 3 cut(s) 249, 366, 679
BsnI GGCC 4 cut(s) 50, 506, 591, 771
Bso31I GGTCTC 2 cut(s) 249, 679
BsoBI CYCGRG 1 cut(s) 859
Bsp1286I GDGCHC 2 cut(s) 216, 810
Bsp143I GATC 2 cut(s) 284, 961
Bsp19I CCATGG 1 cut(s) 772
BspACI CCGC 3 cut(s) 97, 178, 882
BspANI GGCC 4 cut(s) 50, 506, 591, 771
BspCNI CTCAG 1 cut(s) 721
BspFNI CGCG 2 cut(s) 180, 673
BspLI GGNNCC 2 cut(s) 654, 778
BspMAI CTGCAG 1 cut(s) 950
BspPI GGATC 1 cut(s) 292
BspTNI GGTCTC 2 cut(s) 249, 679
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 3 cut(s) 191, 780, 984
BsrI ACTGG 3 cut(s) 372, 497, 764
BssECI CCNNGG 2 cut(s) 656, 772
BssMI GATC 2 cut(s) 284, 961
BssT1I CCWWGG 1 cut(s) 772
Bst2UI CCWGG 3 cut(s) 615, 657, 942
Bst4CI ACNGT 2 cut(s) 350, 577
Bst6I CTCTTC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 665
BstC8I GCNNGC 4 cut(s) 71, 75, 164, 182
BstDEI CTNAG 2 cut(s) 419, 708
BstDSI CCRYGG 1 cut(s) 772
BstF5I GGATG 3 cut(s) 235, 339, 472
BstFNI CGCG 2 cut(s) 180, 673
BstH2I RGCGCY 1 cut(s) 937
BstHHI GCGC 2 cut(s) 673, 936
BstKTI GATC 2 cut(s) 287, 964
BstMAI GTCTC 3 cut(s) 249, 366, 679
BstMBI GATC 2 cut(s) 284, 961
BstMWI GCNNNNNNNGC 7 cut(s) 163, 518, 677, 724, 733, 903, 942
BstNI CCWGG 3 cut(s) 615, 657, 942
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 3 cut(s) 613, 655, 940
BstSFI CTRYAG 1 cut(s) 946
BstSLI GKGCMC 2 cut(s) 216, 810
BstUI CGCG 2 cut(s) 180, 673
BstV1I GCAGC 1 cut(s) 932
BstXI CCANNNNNNTGG 1 cut(s) 46
BsuRI GGCC 4 cut(s) 50, 506, 591, 771
BtgI CCRYGG 1 cut(s) 772
BtsCI GGATG 3 cut(s) 235, 339, 472
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 2 cut(s) 355, 436
Cac8I GCNNGC 4 cut(s) 71, 75, 164, 182
CaiI CAGNNNCTG 1 cut(s) 715
CfoI GCGC 2 cut(s) 673, 936
Csp6I GTAC 2 cut(s) 666, 958
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviQI GTAC 2 cut(s) 666, 958
DdeI CTNAG 2 cut(s) 419, 708
DpnI GATC 2 cut(s) 286, 963
DpnII GATC 2 cut(s) 284, 961
DraI TTTAAA 1 cut(s) 516
DraIII CACNNNGTG 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 372
EaeI YGGCCR 3 cut(s) 48, 504, 769
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 1 cut(s) 591
Eco31I GGTCTC 2 cut(s) 249, 679
Eco88I CYCGRG 1 cut(s) 859
EcoRII CCWGG 3 cut(s) 613, 655, 940
EcoT14I CCWWGG 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 772
Fnu4HI GCNGC 3 cut(s) 178, 883, 946
FokI GGATG 3 cut(s) 242, 326, 479
Fsp4HI GCNGC 3 cut(s) 178, 883, 946
FspBI CTAG 4 cut(s) 258, 485, 603, 920
GlaI GCGC 2 cut(s) 672, 935
GluI GCNGC 3 cut(s) 178, 883, 946
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 937
HaeIII GGCC 4 cut(s) 50, 506, 591, 771
HapII CCGG 1 cut(s) 315
HhaI GCGC 2 cut(s) 673, 936
Hin6I GCGC 2 cut(s) 671, 934
HinP1I GCGC 2 cut(s) 671, 934
HinfI GANTC 8 cut(s) 359, 404, 415, 610, 631, 712, 857, 916
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 5 cut(s) 229, 366, 378, 711, 757
Hpy188III TCNNGA 4 cut(s) 202, 485, 635, 920
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 2 cut(s) 567, 825
HpyCH4III ACNGT 2 cut(s) 350, 577
HpyCH4IV ACGT 3 cut(s) 126, 664, 801
HpyCH4V TGCA 5 cut(s) 122, 132, 521, 785, 948
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 518, 677, 724, 733, 903, 942
HpyF3I CTNAG 2 cut(s) 419, 708
HpySE526I ACGT 3 cut(s) 126, 664, 801
HspAI GCGC 2 cut(s) 671, 934
Kzo9I GATC 2 cut(s) 284, 961
LmnI GCTCC 3 cut(s) 776, 974, 995
Lsp1109I GCAGC 1 cut(s) 932
LweI GCATC 5 cut(s) 348, 457, 499, 685, 993
MaeI CTAG 4 cut(s) 258, 485, 603, 920
MaeII ACGT 3 cut(s) 126, 664, 801
MaeIII GTNAC 3 cut(s) 34, 660, 850
MalI GATC 2 cut(s) 286, 963
MbiI CCGCTC 1 cut(s) 99
MboI GATC 2 cut(s) 284, 961
MboII GAAGA 4 cut(s) 67, 70, 424, 810
MhlI GDGCHC 2 cut(s) 216, 810
MlsI TGGCCA 2 cut(s) 50, 771
MluCI AATT 3 cut(s) 149, 295, 623
MluNI TGGCCA 2 cut(s) 50, 771
MlyI GAGTC 2 cut(s) 368, 851
Mox20I TGGCCA 2 cut(s) 50, 771
MscI TGGCCA 2 cut(s) 50, 771
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 4 cut(s) 348, 790, 877, 969
Msp20I TGGCCA 2 cut(s) 50, 771
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 3 cut(s) 615, 657, 942
MvaI CCWGG 3 cut(s) 615, 657, 942
MvnI CGCG 2 cut(s) 180, 673
MwoI GCNNNNNNNGC 7 cut(s) 163, 518, 677, 724, 733, 903, 942
NcoI CCATGG 1 cut(s) 772
NdeII GATC 2 cut(s) 284, 961
NlaIV GGNNCC 2 cut(s) 654, 778
NmuCI GTSAC 3 cut(s) 34, 660, 850
NspI RCATGY 1 cut(s) 200
PaeR7I CTCGAG 1 cut(s) 859
PceI AGGCCT 1 cut(s) 591
PciI ACATGT 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 670
PfeI GAWTC 6 cut(s) 404, 415, 610, 631, 712, 916
Pfl23II CGTACG 1 cut(s) 665
PflMI CCANNNNNTGG 1 cut(s) 786
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 3 cut(s) 179, 884, 947
PleI GAGTC 2 cut(s) 367, 851
PpsI GAGTC 2 cut(s) 367, 851
Ppu21I YACGTR 1 cut(s) 665
PscI ACATGT 1 cut(s) 196
Psp6I CCWGG 3 cut(s) 613, 655, 940
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 3 cut(s) 613, 655, 940
PspLI CGTACG 1 cut(s) 665
PspN4I GGNNCC 2 cut(s) 654, 778
PspXI VCTCGAGB 1 cut(s) 859
PstI CTGCAG 1 cut(s) 950
PstNI CAGNNNCTG 1 cut(s) 715
RsaI GTAC 2 cut(s) 667, 959
RsaNI GTAC 2 cut(s) 666, 958
RseI CAYNNNNRTG 4 cut(s) 348, 790, 877, 969
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 3 cut(s) 178, 883, 946
Sau3AI GATC 2 cut(s) 284, 961
SchI GAGTC 2 cut(s) 368, 851
ScrFI CCNGG 3 cut(s) 615, 657, 942
SduI GDGCHC 2 cut(s) 216, 810
SfaNI GCATC 5 cut(s) 348, 457, 499, 685, 993
SfcI CTRYAG 1 cut(s) 946
Sfr274I CTCGAG 1 cut(s) 859
SlaI CTCGAG 1 cut(s) 859
SmiMI CAYNNNNRTG 4 cut(s) 348, 790, 877, 969
SmlI CTYRAG 1 cut(s) 859
SmoI CTYRAG 1 cut(s) 859
Sse9I AATT 3 cut(s) 149, 295, 623
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 3 cut(s) 97, 178, 882
SspMI CTAG 4 cut(s) 258, 485, 603, 920
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 613, 655, 940
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 2 cut(s) 350, 577
TaiI ACGT 3 cut(s) 129, 667, 804
TaqI TCGA 5 cut(s) 168, 335, 407, 629, 860
TasI AATT 3 cut(s) 149, 295, 623
TauI GCSGC 2 cut(s) 180, 885
TfiI GAWTC 6 cut(s) 404, 415, 610, 631, 712, 916
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TscAI CASTG 2 cut(s) 355, 436
TseFI GTSAC 3 cut(s) 34, 660, 850
TseI GCWGC 1 cut(s) 945
Tsp45I GTSAC 3 cut(s) 34, 660, 850
TspDTI ATGAA 3 cut(s) 425, 810, 867
TspRI CASTG 2 cut(s) 355, 436
Van91I CCANNNNNTGG 1 cut(s) 786
XbaI TCTAGA 2 cut(s) 484, 919
XceI RCATGY 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 859
XspI CTAG 4 cut(s) 258, 485, 603, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.