Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0010693

Physical Location & Seq

Genomic Coordinates Reverse (-)

3580372 .. 3583323

Gene Structure 2952 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGTCTCACAGTCCCGGCTGCTCTTCTGTGGCATATGGTGCATCGGAAGAGATAGGACCATTTAGAATAAACAAGAGTGCTTCAGGACTAAACTTGAACAAGTTCTCATGGAACACAGTTGCCAATCTCTTGTTCTTGGAAACTCCAGCCGGGGTCGGCTTCTCCTACAGCAACCGCTCCTCTGATCTCTTTGACACCGGTGATCGCCGCACTGCAAAGGACTCATTGCAATTCATAATCCGATGGCTGGATCGATTCCCACGATACAAGGGCAGAGAAGTATATCTCACAGGCGAGAGCTATGCAGGACATTATGTTCCTCAGCTAGCCAAAGCAATTTTGACATACAATTCACAGTCCAAACACCCAATCAATCTTAAAGGAATAATGGTGGGTAATGCAGTGACAGACAACTACTACGATAACCTAGGAACGGTGACATACTGGTGGAGCCATGCAATGATCTCCGATAAAACCTACAAACAACTGATCAACACATGCGATTTTAGGAGGCAGAAGAACTCAGATCAATGTGAGTCGTTGTATAGCTACGCCATGGATAAAGAGTTTGGAAACATAGACCAGTATAACATTTACGCACCACCATGCAACAACTCCGATGGAAGCACAAGAACTTCTACAAGGCAGACTGCTATGCATTTGCCTCATCGACCGATGTTCCGGCAAATATCCGGCTATGATCCTTGTACGGAGAAATATGCTGAACTTTATTACAACAGGCCAGATGTACAGAAAGCACTCCATGCCAACATAACCAAAATTCCTTATAAGTGGACTGCTTGCAGTGAGGTTTTAAATCGAAACTGGAACGACACAGATGTATCGGTTCTTCCTATTTACAGGGATATGATAACTACTGGTTTAAGGATTTGGGTTTTCAGTGGAGATGTGGACTCAGTGGTGCCAGTCACAGCCACTAGATATTCCCTTGCACAACTCAAATTGGCCACCAAAATTCCATGGTACCCCTGGTATACTAAGAAGCAGGTCGGAGGGTGGACAGAGGTTTACGAAGGGCTGACATTTGCAACAGTGAGAGGAGCAGGTCATGAAGTTCCACTTTTCAAGCCAAGAGCAGCTCTTGAGCTTTTCAGATCATTTATTCAAGGGAGGCCTCTTCCCAAATCCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005576 GO:0005615 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016787 GO:0017171 GO:0019538 GO:0023052 GO:0030163 GO:0032870 GO:0033993 GO:0042221 GO:0043170 GO:0043401 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0065007 GO:0070008 GO:0070011 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

385

Amino Acids

43.77

Weight (kDa)

9.13

Isoelectric Point (pI)

42.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	5 - 377	8e-122	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010693)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02110
fragaria_vesca	FvH4_6g46890 FvH4_6g46890 FvH4_6g46890 FvH4_6g46890
malus_domestica	MD09G1066700.v1.1
prunus_persica	Prupe.3G255000_v2.0.a1 Prupe.3G255000_v2.0.a1
pyrus_communis	pycom111g05590
rosa_chinensis	RchiOBHm_Chr2g0165861
rosa_laevigata	RLG00000021562
rosa_multiflora	Rmu_co8359035.1_g000001 Rmu_sc0008709.1_g000008
rosa_roxburghii	Rroxscaffold_2G00085410
rosa_rugosa	Rorug02G0518400
rosa_samantha	Rh2AG584200 Rh2AG584300 Rh2BG596100 Rh2CG567000
rosa_wichuraiana	Rw2G048710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	789
Acc36I	ACCTGC	2 cut(s)	997, 1055
Acc65I	GGTACC	1 cut(s)	984
AccB1I	GGYRCC	2 cut(s)	922, 984
AccBSI	CCGCTC	1 cut(s)	177
AccI	GTMKAC	1 cut(s)	995
AciI	CCGC	2 cut(s)	175, 208
AclWI	GGATC	2 cut(s)	258, 695
AcoI	YGGCCR	1 cut(s)	966
AcsI	RAATTY	2 cut(s)	780, 975
AcuI	CTGAAG	1 cut(s)	66
AfaI	GTAC	3 cut(s)	709, 750, 986
AfiI	CCNNNNNNNGG	2 cut(s)	247, 433
AgeI	ACCGGT	1 cut(s)	197
AgsI	TTSAA	3 cut(s)	97, 1087, 1127
AjnI	CCWGG	1 cut(s)	989
AjuI	GAANNNNNNNTTGG	2 cut(s)	116, 148
AloI	GAACNNNNNNTCC	2 cut(s)	300, 332
AluBI	AGCT	5 cut(s)	300, 325, 549, 1100, 1108
AluI	AGCT	5 cut(s)	300, 325, 549, 1100, 1108
Alw26I	GTCTC	1 cut(s)	9
AlwI	GGATC	2 cut(s)	258, 695
AoxI	GGCC	3 cut(s)	740, 966, 1133
ApeKI	GCWGC	2 cut(s)	18, 1097
ApoI	RAATTY	2 cut(s)	780, 975
AsiGI	ACCGGT	1 cut(s)	197
Asp700I	GAANNNNTTC	1 cut(s)	101
Asp718I	GGTACC	1 cut(s)	984
AspA2I	CCTAGG	1 cut(s)	427
AspS9I	GGNCC	1 cut(s)	56
AsuC2I	CCSGG	2 cut(s)	15, 151
AsuHPI	GGTGA	2 cut(s)	212, 448
AsuNHI	GCTAGC	1 cut(s)	325
AvaII	GGWCC	1 cut(s)	56
AvrII	CCTAGG	1 cut(s)	427
BaeI	ACNNNNGTAYC	2 cut(s)	825, 858
BalI	TGGCCA	1 cut(s)	968
BanI	GGYRCC	2 cut(s)	922, 984
BarI	GAAGNNNNNNTAC	2 cut(s)	834, 866
BbvCI	CCTCAGC	1 cut(s)	321
BbvI	GCAGC	2 cut(s)	5, 1109
BccI	CCATC	2 cut(s)	237, 614
BcgI	CGANNNNNNTGC	2 cut(s)	284, 318
BciT130I	CCWGG	1 cut(s)	991
BclI	TGATCA	1 cut(s)	489
BcnI	CCSGG	2 cut(s)	15, 151
BcoDI	GTCTC	1 cut(s)	9
BfaI	CTAG	3 cut(s)	326, 428, 939
BfmI	CTRYAG	1 cut(s)	166
BfuAI	ACCTGC	2 cut(s)	997, 1055
BisI	GCNGC	3 cut(s)	19, 208, 1098
BlnI	CCTAGG	1 cut(s)	427
BlsI	GCNGC	3 cut(s)	20, 209, 1099
Bme1390I	CCNGG	3 cut(s)	15, 151, 991
Bme18I	GGWCC	1 cut(s)	56
BmgT120I	GGNCC	1 cut(s)	56
BmiI	GGNNCC	3 cut(s)	452, 924, 986
BmrFI	CCNGG	3 cut(s)	15, 151, 991
BmsI	GCATC	1 cut(s)	50
BmtI	GCTAGC	1 cut(s)	329
BpmI	CTGGAG	1 cut(s)	129
Bpu10I	CCTNAGC	1 cut(s)	321
BpuEI	CTTGAG	1 cut(s)	1124
BpuMI	CCSGG	2 cut(s)	15, 151
Bsa29I	ATCGAT	1 cut(s)	253
BsaJI	CCNNGG	5 cut(s)	150, 427, 555, 980, 989
BsaWI	WCCGGW	1 cut(s)	197
Bsc4I	CCNNNNNNNGG	2 cut(s)	247, 433
Bse118I	RCCGGY	1 cut(s)	197
Bse1I	ACTGG	5 cut(s)	449, 583, 830, 883, 926
Bse3DI	GCAATG	2 cut(s)	224, 465
BseBI	CCWGG	1 cut(s)	991
BseCI	ATCGAT	1 cut(s)	253
BseDI	CCNNGG	5 cut(s)	150, 427, 555, 980, 989
BseLI	CCNNNNNNNGG	2 cut(s)	247, 433
BseMI	GCAATG	2 cut(s)	224, 465
BseMII	CTCAG	3 cut(s)	335, 537, 930
BseNI	ACTGG	5 cut(s)	449, 583, 830, 883, 926
BseRI	GAGGAG	2 cut(s)	169, 1074
BseXI	GCAGC	2 cut(s)	5, 1109
Bsh1285I	CGRYCG	1 cut(s)	674
BshFI	GGCC	3 cut(s)	742, 968, 1135
BshNI	GGYRCC	2 cut(s)	922, 984
BshTI	ACCGGT	1 cut(s)	197
BshVI	ATCGAT	1 cut(s)	253
BsiEI	CGRYCG	1 cut(s)	674
BsiSI	CCGG	5 cut(s)	15, 150, 198, 682, 693
BslI	CCNNNNNNNGG	2 cut(s)	247, 433
BsmAI	GTCTC	1 cut(s)	9
BsnI	GGCC	3 cut(s)	742, 968, 1135
Bsp1407I	TGTACA	1 cut(s)	748
Bsp143I	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
Bsp19I	CCATGG	2 cut(s)	555, 980
BspACI	CCGC	2 cut(s)	175, 208
BspANI	GGCC	3 cut(s)	742, 968, 1135
BspCNI	CTCAG	3 cut(s)	334, 536, 929
BspDI	ATCGAT	1 cut(s)	253
BspHI	TCATGA	2 cut(s)	1069, 1151
BspLI	GGNNCC	3 cut(s)	452, 924, 986
BspMI	ACCTGC	2 cut(s)	997, 1055
BspOI	GCTAGC	1 cut(s)	329
BspPI	GGATC	2 cut(s)	258, 695
BspQI	GCTCTTC	1 cut(s)	28
BspT107I	GGYRCC	2 cut(s)	922, 984
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	224, 465
BsrFI	RCCGGY	1 cut(s)	197
BsrGI	TGTACA	1 cut(s)	748
BsrI	ACTGG	5 cut(s)	449, 583, 830, 883, 926
BssAI	RCCGGY	1 cut(s)	197
BssECI	CCNNGG	5 cut(s)	150, 427, 555, 980, 989
BssMI	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
BssNAI	GTATAC	1 cut(s)	996
BssT1I	CCWWGG	3 cut(s)	427, 555, 980
Bst1107I	GTATAC	1 cut(s)	996
Bst2UI	CCWGG	1 cut(s)	991
Bst4CI	ACNGT	5 cut(s)	11, 118, 357, 436, 1054
Bst6I	CTCTTC	3 cut(s)	28, 42, 1143
BstAPI	GCANNNNNTGC	2 cut(s)	38, 764
BstAUI	TGTACA	1 cut(s)	748
BstC8I	GCNNGC	2 cut(s)	327, 802
BstDEI	CTNAG	4 cut(s)	321, 523, 916, 999
BstDSI	CCRYGG	2 cut(s)	555, 980
BstKTI	GATC	8 cut(s)	187, 205, 253, 465, 492, 529, 703, 1118
BstMAI	GTCTC	1 cut(s)	9
BstMBI	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
BstMCI	CGRYCG	1 cut(s)	674
BstMWI	GCNNNNNNNGC	2 cut(s)	38, 764
BstNI	CCWGG	1 cut(s)	991
BstNSI	RCATGY	1 cut(s)	501
BstSCI	CCNGG	3 cut(s)	13, 149, 989
BstSFI	CTRYAG	1 cut(s)	166
BstV1I	GCAGC	2 cut(s)	5, 1109
BstZ17I	GTATAC	1 cut(s)	996
Bsu15I	ATCGAT	1 cut(s)	253
BsuRI	GGCC	3 cut(s)	742, 968, 1135
BsuTUI	ATCGAT	1 cut(s)	253
BtgI	CCRYGG	2 cut(s)	555, 980
BtsI	GCAGTG	3 cut(s)	210, 408, 811
BtsIMutI	CAGTG	6 cut(s)	210, 408, 811, 907, 924, 1059
BveI	ACCTGC	2 cut(s)	997, 1055
Cac8I	GCNNGC	2 cut(s)	327, 802
CciI	TCATGA	2 cut(s)	1069, 1151
Cfr10I	RCCGGY	1 cut(s)	197
Cfr13I	GGNCC	1 cut(s)	56
ClaI	ATCGAT	1 cut(s)	253
Csp6I	GTAC	3 cut(s)	708, 749, 985
CspAI	ACCGGT	1 cut(s)	197
CviAII	CATG	9 cut(s)	108, 455, 498, 556, 606, 764, 981, 1070, 1152
CviQI	GTAC	3 cut(s)	708, 749, 985
DdeI	CTNAG	4 cut(s)	321, 523, 916, 999
DpnI	GATC	8 cut(s)	186, 204, 252, 464, 491, 528, 702, 1117
DpnII	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
DraI	TTTAAA	1 cut(s)	816
EaeI	YGGCCR	1 cut(s)	966
Eam1104I	CTCTTC	3 cut(s)	28, 42, 1143
EarI	CTCTTC	3 cut(s)	28, 42, 1143
Eco130I	CCWWGG	3 cut(s)	427, 555, 980
Eco147I	AGGCCT	1 cut(s)	1135
Eco47I	GGWCC	1 cut(s)	56
Eco57I	CTGAAG	1 cut(s)	66
EcoRII	CCWGG	1 cut(s)	989
EcoT14I	CCWWGG	3 cut(s)	427, 555, 980
EcoT22I	ATGCAT	1 cut(s)	660
ErhI	CCWWGG	3 cut(s)	427, 555, 980
FaeI	CATG	9 cut(s)	111, 458, 501, 559, 609, 767, 984, 1073, 1155
FalI	AAGNNNNNCTT	2 cut(s)	1065, 1097
FatI	CATG	9 cut(s)	107, 454, 497, 555, 605, 763, 980, 1069, 1151
FauNDI	CATATG	1 cut(s)	34
FbaI	TGATCA	1 cut(s)	489
FblI	GTMKAC	1 cut(s)	995
Fnu4HI	GCNGC	3 cut(s)	19, 208, 1098
Fsp4HI	GCNGC	3 cut(s)	19, 208, 1098
FspBI	CTAG	3 cut(s)	326, 428, 939
GluI	GCNGC	3 cut(s)	19, 208, 1098
GsuI	CTGGAG	1 cut(s)	129
HaeIII	GGCC	3 cut(s)	742, 968, 1135
HapII	CCGG	5 cut(s)	15, 150, 198, 682, 693
Hin1II	CATG	9 cut(s)	111, 458, 501, 559, 609, 767, 984, 1073, 1155
HinfI	GANTC	4 cut(s)	221, 255, 536, 914
HpaII	CCGG	5 cut(s)	15, 150, 198, 682, 693
HphI	GGTGA	2 cut(s)	212, 448
Hpy166II	GTNNAC	5 cut(s)	795, 913, 996, 1020, 1030
Hpy188I	TCNGA	8 cut(s)	46, 184, 242, 469, 526, 619, 1013, 1115
Hpy188III	TCNNGA	4 cut(s)	84, 1070, 1103, 1152
Hpy8I	GTNNAC	5 cut(s)	795, 913, 996, 1020, 1030
HpyAV	CCTTC	1 cut(s)	1028
HpyCH4III	ACNGT	5 cut(s)	11, 118, 357, 436, 1054
HpyF10VI	GCNNNNNNNGC	2 cut(s)	38, 764
HpyF3I	CTNAG	4 cut(s)	321, 523, 916, 999
Hsp92II	CATG	9 cut(s)	111, 458, 501, 559, 609, 767, 984, 1073, 1155
KpnI	GGTACC	1 cut(s)	988
Ksp22I	TGATCA	1 cut(s)	489
Kzo9I	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
LguI	GCTCTTC	1 cut(s)	28
LmnI	GCTCC	3 cut(s)	182, 450, 1061
Lsp1109I	GCAGC	2 cut(s)	5, 1109
LweI	GCATC	1 cut(s)	50
MaeI	CTAG	3 cut(s)	326, 428, 939
MaeIII	GTNAC	3 cut(s)	403, 436, 928
MalI	GATC	8 cut(s)	186, 204, 252, 464, 491, 528, 702, 1117
MbiI	CCGCTC	1 cut(s)	177
MboI	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
MboII	GAAGA	5 cut(s)	15, 59, 529, 842, 1130
MlsI	TGGCCA	1 cut(s)	968
MluCI	AATT	6 cut(s)	230, 336, 349, 780, 962, 975
MluNI	TGGCCA	1 cut(s)	968
MlyI	GAGTC	3 cut(s)	215, 545, 908
MmeI	TCCRAC	1 cut(s)	991
Mox20I	TGGCCA	1 cut(s)	968
Mph1103I	ATGCAT	1 cut(s)	660
MroXI	GAANNNNTTC	1 cut(s)	101
MscI	TGGCCA	1 cut(s)	968
MseI	TTAA	3 cut(s)	378, 815, 884
MslI	CAYNNNNRTG	2 cut(s)	445, 604
Msp20I	TGGCCA	1 cut(s)	968
MspI	CCGG	5 cut(s)	15, 150, 198, 682, 693
MspR9I	CCNGG	3 cut(s)	15, 151, 991
MvaI	CCWGG	1 cut(s)	991
MwoI	GCNNNNNNNGC	2 cut(s)	38, 764
NciI	CCSGG	2 cut(s)	15, 151
NcoI	CCATGG	2 cut(s)	555, 980
NdeI	CATATG	1 cut(s)	34
NdeII	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
NheI	GCTAGC	1 cut(s)	325
NlaIII	CATG	9 cut(s)	111, 458, 501, 559, 609, 767, 984, 1073, 1155
NlaIV	GGNNCC	3 cut(s)	452, 924, 986
NmuCI	GTSAC	3 cut(s)	403, 436, 928
NsiI	ATGCAT	1 cut(s)	660
NspI	RCATGY	1 cut(s)	501
PagI	TCATGA	2 cut(s)	1069, 1151
PceI	AGGCCT	1 cut(s)	1135
PciSI	GCTCTTC	1 cut(s)	28
PcsI	WCGNNNNNNNCGW	1 cut(s)	259
PdmI	GAANNNNTTC	1 cut(s)	101
PfeI	GAWTC	1 cut(s)	255
PinAI	ACCGGT	1 cut(s)	197
PkrI	GCNGC	3 cut(s)	20, 209, 1099
PleI	GAGTC	3 cut(s)	215, 544, 908
PpsI	GAGTC	3 cut(s)	215, 544, 908
PsiI	TTATAA	1 cut(s)	789
Psp6I	CCWGG	1 cut(s)	989
PspGI	CCWGG	1 cut(s)	989
PspN4I	GGNNCC	3 cut(s)	452, 924, 986
PspPI	GGNCC	1 cut(s)	56
RsaI	GTAC	3 cut(s)	709, 750, 986
RsaNI	GTAC	3 cut(s)	708, 749, 985
RseI	CAYNNNNRTG	2 cut(s)	445, 604
SapI	GCTCTTC	1 cut(s)	28
SaqAI	TTAA	3 cut(s)	378, 815, 884
SatI	GCNGC	3 cut(s)	19, 208, 1098
Sau3AI	GATC	8 cut(s)	184, 202, 250, 462, 489, 526, 700, 1115
Sau96I	GGNCC	1 cut(s)	56
SchI	GAGTC	3 cut(s)	215, 545, 908
ScrFI	CCNGG	3 cut(s)	15, 151, 991
SfaNI	GCATC	1 cut(s)	50
SfcI	CTRYAG	1 cut(s)	166
SgrAI	CRCCGGYG	1 cut(s)	197
SinI	GGWCC	1 cut(s)	56
SmiMI	CAYNNNNRTG	2 cut(s)	445, 604
SmlI	CTYRAG	1 cut(s)	1103
SmoI	CTYRAG	1 cut(s)	1103
Sse9I	AATT	6 cut(s)	230, 336, 349, 780, 962, 975
SseBI	AGGCCT	1 cut(s)	1135
SsiI	CCGC	2 cut(s)	175, 208
SspMI	CTAG	3 cut(s)	326, 428, 939
StuI	AGGCCT	1 cut(s)	1135
StyD4I	CCNGG	3 cut(s)	13, 149, 989
StyI	CCWWGG	3 cut(s)	427, 555, 980
TaaI	ACNGT	5 cut(s)	11, 118, 357, 436, 1054
TaqI	TCGA	3 cut(s)	253, 670, 820
TaqII	GACCGA	1 cut(s)	688
TasI	AATT	6 cut(s)	230, 336, 349, 780, 962, 975
TatI	WGTACW	1 cut(s)	748
TauI	GCSGC	1 cut(s)	210
TfiI	GAWTC	1 cut(s)	255
Tru1I	TTAA	3 cut(s)	378, 815, 884
Tru9I	TTAA	3 cut(s)	378, 815, 884
TscAI	CASTG	6 cut(s)	217, 408, 811, 907, 924, 1059
TseFI	GTSAC	3 cut(s)	403, 436, 928
TseI	GCWGC	2 cut(s)	18, 1097
Tsp45I	GTSAC	3 cut(s)	403, 436, 928
TspDTI	ATGAA	2 cut(s)	223, 1086
TspGWI	ACGGA	1 cut(s)	725
TspRI	CASTG	6 cut(s)	217, 408, 811, 907, 924, 1059
VpaK11BI	GGWCC	1 cut(s)	56
XapI	RAATTY	2 cut(s)	780, 975
XceI	RCATGY	1 cut(s)	501
XcmI	CCANNNNNNNNNTGG	1 cut(s)	987
XmaJI	CCTAGG	1 cut(s)	427
XmiI	GTMKAC	1 cut(s)	995
XmnI	GAANNNNTTC	1 cut(s)	101
XspI	CTAG	3 cut(s)	326, 428, 939
Zsp2I	ATGCAT	1 cut(s)	660

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.