pycom11g05520
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007317
Physical Location & Seq
Forward (+)
4190099 .. 4192318
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGCGTCCGCTCGGCTTGAAAGTAGAACCCTTTCTTTTTCTCTTATGTCCTGCCTCGTCTTGGTTGCTGTGTTCACACCCCCTTCTTCTGCAGCCAGAACTCCACCTTCAGATTCTGGTAAAGTTTCGTTGGCTTTGTATTATGAGTCCCTCTGCCCCTACAGTGCCAACTTCATTGTGAATTACCTTGTCAAGCTCTTCGAAGATGACCTGATCTCCATTGTTGATCTCAAGCTTTCTCCTTGGGGTAATGCCAAGCTCAGAAGCAACGACACATTCACTTGCCAGCATGGTCCGTCTGAATGTTTATTGAACACTGTGGAAGCCTGTGCGATCGAGATCTGGCCTGCATTGAACGATCATTTTCCTTTCATTTATTGCGTCGAGAGTTTGGTTTACAAGCACAAGTATCCCCAGTGGGAGTCATGTTACGAGAAACTGGGCTTGGATTCAAAACCTATTGCTGAATGCTACAGCAGTGGACTTGGTAAAGAGCTTGAACTACAGTATGCAGCTGAAACCAGTGCACTCCAGCCTCCGCATCAGTATGTGCCATGGGTAGTTGTGGATGGACAGCCACTTTATGAGGACTACGAAAACTTCCTGAGCTATGTCTGCAATGCCTATAATGGCACCACTGCCCTCGAGGCCTGCAGTAAAATATCCCTCAATACCAGGAAAAGTTCCAAATCTACCCATTCCGTTGGCTGCGAGGGAAATATGCCAGCAGTATTAGGAAGAATAGGATCGACCATAAAATCGTGGATCCGTCAAATGAACCTGGCAATTTGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

266

Amino Acids

29.5

Weight (kDa)

5.41

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 44 - 146 3.1e-32 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 632
AccBSI CCGCTC 1 cut(s) 11
AciI CCGC 2 cut(s) 9, 539
AclWI GGATC 3 cut(s) 754, 760, 773
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 5 cut(s) 20, 313, 355, 453, 500
AjnI CCWGG 2 cut(s) 674, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 6 cut(s) 196, 235, 259, 496, 515, 609
AluI AGCT 6 cut(s) 196, 235, 259, 496, 515, 609
Alw21I GWGCWC 1 cut(s) 529
Alw44I GTGCAC 1 cut(s) 525
AlwI GGATC 3 cut(s) 754, 760, 773
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 2 cut(s) 344, 648
ApaLI GTGCAC 1 cut(s) 525
ApeKI GCWGC 3 cut(s) 92, 512, 708
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 293
AsuII TTCGAA 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 529
BamHI GGATCC 1 cut(s) 765
BanI GGYRCC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 3 cut(s) 104, 524, 695
BccI CCATC 1 cut(s) 563
BciT130I CCWGG 2 cut(s) 676, 782
BciVI GTATCC 1 cut(s) 420
BfmI CTRYAG 5 cut(s) 90, 160, 472, 503, 652
BfuI GTATCC 1 cut(s) 420
BglI GCCNNNNNGGC 1 cut(s) 647
BglII AGATCT 1 cut(s) 339
BisI GCNGC 3 cut(s) 93, 513, 709
BlsI GCNGC 3 cut(s) 94, 514, 710
Bme1390I CCNGG 2 cut(s) 676, 782
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 634, 767
BmrFI CCNGG 2 cut(s) 676, 782
BmrI ACTGGG 2 cut(s) 409, 449
BmsI GCATC 1 cut(s) 550
BmuI ACTGGG 2 cut(s) 409, 449
BpmI CTGGAG 1 cut(s) 515
Bpu10I CCTNAGC 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 242, 554
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 3 cut(s) 415, 444, 522
Bse3DI GCAATG 1 cut(s) 625
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 2 cut(s) 676, 782
BseDI CCNNGG 2 cut(s) 242, 554
BseGI GGATG 2 cut(s) 574, 798
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 2 cut(s) 274, 596
BseNI ACTGG 3 cut(s) 415, 444, 522
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 3 cut(s) 104, 524, 695
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 2 cut(s) 346, 650
BshNI GGYRCC 1 cut(s) 632
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 644
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 2 cut(s) 346, 650
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 2 cut(s) 9, 539
BspANI GGCC 2 cut(s) 346, 650
BspCNI CTCAG 2 cut(s) 273, 597
BspLI GGNNCC 2 cut(s) 634, 767
BspMAI CTGCAG 2 cut(s) 94, 656
BspPI GGATC 3 cut(s) 754, 760, 773
BspQI GCTCTTC 1 cut(s) 203
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 625
BsrI ACTGG 3 cut(s) 415, 444, 522
BssECI CCNNGG 2 cut(s) 242, 554
BssMI GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 242, 554
Bst2UI CCWGG 2 cut(s) 676, 782
Bst4CI ACNGT 3 cut(s) 164, 319, 507
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 287, 348, 652, 726
BstDEI CTNAG 2 cut(s) 260, 605
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 574, 798
BstKTI GATC 7 cut(s) 216, 229, 336, 342, 361, 749, 768
BstMBI GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 2 cut(s) 615, 647
BstNI CCWGG 2 cut(s) 676, 782
BstSCI CCNGG 2 cut(s) 674, 780
BstSFI CTRYAG 5 cut(s) 90, 160, 472, 503, 652
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 3 cut(s) 104, 524, 695
BstX2I RGATCY 2 cut(s) 339, 765
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 2 cut(s) 339, 765
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 346, 650
BtgI CCRYGG 1 cut(s) 554
BtsCI GGATG 2 cut(s) 574, 798
BtsI GCAGTG 2 cut(s) 484, 636
BtsIMutI CAGTG 6 cut(s) 169, 315, 422, 484, 529, 636
Cac8I GCNNGC 4 cut(s) 287, 348, 652, 726
CaiI CAGNNNCTG 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 370
CviAII CATG 3 cut(s) 290, 426, 555
DdeI CTNAG 2 cut(s) 260, 605
DpnI GATC 7 cut(s) 215, 228, 335, 341, 360, 748, 767
DpnII GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 242, 554
Eco147I AGGCCT 1 cut(s) 650
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 644
EcoRII CCWGG 2 cut(s) 674, 780
EcoT14I CCWWGG 2 cut(s) 242, 554
ErhI CCWWGG 2 cut(s) 242, 554
FaeI CATG 3 cut(s) 293, 429, 558
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 289, 425, 554
Fnu4HI GCNGC 3 cut(s) 93, 513, 709
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 3 cut(s) 93, 513, 709
GluI GCNGC 3 cut(s) 93, 513, 709
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 2 cut(s) 346, 650
HgaI GACGC 1 cut(s) 370
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 293, 429, 558
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 4 cut(s) 113, 146, 422, 449
Hpy166II GTNNAC 4 cut(s) 75, 397, 482, 527
Hpy188I TCNGA 3 cut(s) 112, 263, 301
Hpy188III TCNNGA 3 cut(s) 337, 385, 604
Hpy8I GTNNAC 4 cut(s) 75, 397, 482, 527
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 2 cut(s) 93, 117
HpyCH4III ACNGT 3 cut(s) 164, 319, 507
HpyCH4V TGCA 6 cut(s) 92, 350, 512, 527, 618, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 615, 647
HpyF3I CTNAG 2 cut(s) 260, 605
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 293, 429, 558
Kzo9I GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
LguI GCTCTTC 1 cut(s) 203
Lsp1109I GCAGC 3 cut(s) 104, 524, 695
LweI GCATC 1 cut(s) 550
MaeIII GTNAC 1 cut(s) 428
MalI GATC 7 cut(s) 215, 228, 335, 341, 360, 748, 767
MbiI CCGCTC 1 cut(s) 11
MboI GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
MboII GAAGA 4 cut(s) 78, 190, 215, 750
MflI RGATCY 2 cut(s) 339, 765
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 2 cut(s) 181, 786
MlyI GAGTC 2 cut(s) 155, 431
MnlI CCTC 8 cut(s) 65, 161, 546, 580, 640, 653, 677, 706
MroXI GAANNNNTTC 1 cut(s) 31
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 676, 782
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 2 cut(s) 676, 782
MwoI GCNNNNNNNGC 2 cut(s) 615, 647
NcoI CCATGG 1 cut(s) 554
NdeII GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
NlaIII CATG 3 cut(s) 293, 429, 558
NlaIV GGNNCC 2 cut(s) 634, 767
NspV TTCGAA 1 cut(s) 201
PaeR7I CTCGAG 1 cut(s) 644
PceI AGGCCT 1 cut(s) 650
PciSI GCTCTTC 1 cut(s) 203
PctI GAATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 113, 449
PkrI GCNGC 3 cut(s) 94, 514, 710
Ple19I CGATCG 1 cut(s) 336
PleI GAGTC 2 cut(s) 154, 430
PpsI GAGTC 2 cut(s) 154, 430
Psp6I CCWGG 2 cut(s) 674, 780
PspGI CCWGG 2 cut(s) 674, 780
PspN4I GGNNCC 2 cut(s) 634, 767
PspPI GGNCC 1 cut(s) 293
PspXI VCTCGAGB 1 cut(s) 644
PstI CTGCAG 2 cut(s) 94, 656
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 2 cut(s) 339, 765
PvuI CGATCG 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 515
RseI CAYNNNNRTG 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 203
SatI GCNGC 3 cut(s) 93, 513, 709
Sau3AI GATC 7 cut(s) 213, 226, 333, 339, 358, 746, 765
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 155, 431
ScrFI CCNGG 2 cut(s) 676, 782
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 5 cut(s) 90, 160, 472, 503, 652
Sfr274I CTCGAG 1 cut(s) 644
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 2 cut(s) 230, 644
SmoI CTYRAG 2 cut(s) 230, 644
Sse9I AATT 2 cut(s) 181, 786
SseBI AGGCCT 1 cut(s) 650
SsiI CCGC 2 cut(s) 9, 539
StuI AGGCCT 1 cut(s) 650
StyD4I CCNGG 2 cut(s) 674, 780
StyI CCWWGG 2 cut(s) 242, 554
TaaI ACNGT 3 cut(s) 164, 319, 507
TaqI TCGA 5 cut(s) 201, 336, 384, 645, 749
TasI AATT 2 cut(s) 181, 786
TfiI GAWTC 2 cut(s) 113, 449
TscAI CASTG 6 cut(s) 169, 322, 422, 484, 529, 643
TseI GCWGC 3 cut(s) 92, 512, 708
TspDTI ATGAA 3 cut(s) 163, 361, 791
TspGWI ACGGA 3 cut(s) 285, 691, 758
TspRI CASTG 6 cut(s) 169, 322, 422, 484, 529, 643
VneI GTGCAC 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.