pycom11g06050

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011766
Physical Location & Seq
Reverse (-)
4665157 .. 4667043
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1887 bp
ATGATCTCCGCTATGTTCGGTAGCATGAACGTGTTGAATTATATTCTTCAATCCTGTCTTGTTGATGTTAATAAAGCCTGCGGTTCAGATAGAGCTACTGCTCTTCATTGTGCTGTTGCTGGTGGCTCTGCTGCTTCGGCTGAGGTTGTCAAGCTCTTGCTTGCTGCTTCTGCTGATGCGAATTCTCTTGATGCTAATGGAAACCAACCAGGTGACTTGATCGCACCTGCTTATAGTTCGAGCTTCAGTTCGAGGAAGAAAGCTTTGGAGGTCATGCTCAAGGGTGTTCCCAGTATTGATGAACCTTTTGACTTCTCGGAGCAAATGATTAATGAGACAGAAGGGCAAGAACAGCAAGAGATGACAACTCCTCGTGCTTCAAAGGATGGAACAGAGAAGAAAGAGTATCCTGTTGATCTTTCTCTTCCGGACATCAAGAACGGGATTTATAGCACGGATGAGTTTAGAATGTATACTTTCAAGGTCAAGCCGTGCTCAAGGGCCTACTCTCATGACTGGACAGAGTGTCCATTCGTCCACCCGGGGGAAAATGCAAGGCGGCGTGATCCAAGGAAATATCATTACAGCTGTGTTCCTTGCCCGGAGTTCAGAAAGGGGACCTGCAGGCAGGGAGATGCTTGTGAATATGCACATGGTATTTTTGAATGTTGGCTTCACCCTGCCCAGTACCGTACCCGTCTTTGCAAGGATGAGACAGGATGCACAAGAAGGGTCTGTTTCTTTGCTCACAAGCCAGAGGAGCTTCGCCCCTTGTATGCTTCGACAGGTTCTGCTGTGCCTTCTCCTAGATCATTCTCAGCCACTGCTGCTTCTCTGGACATGGGGTCAATTGCCCCGCTTTCCCTCAACTCTCCATCCATGATGATTCCTCCTGCTTCAACTCCGCCCATGACTCCCACTGGACCTTCTTCTCCTATGAGTGGAAACATGTGGCAGAACAATCCAAACTTTGCACCCCCCACTTTGCAGCTTCCAGGTAGCAGATTGAAATCTACTCTGAGTGCTAGAGATATGGATTTTGAGATCGGGATGCTTAGCCTGGAGAGGGATCGTCGAAGGCAGCAACGCTTGATTGATGAGATATCCGGTTCCCCATCTAGCTGGAACAAAGTCTTATCTCCTGTGTCGCCCTTTTCTGCCTCTGGGAATCGAACAGGGGAATTGAATACCATTGGAGGAGTGAACCCTACTAACCTTGACGACATTTTTGGATCTCTTGATCCTGCAATTTTGCCTCAATTTAATGGCCTTTCACGGGATGCAACAGCATCCCAGTTACATTCTCCAACTGGGATCCAGATGCGCCAAAATATGAACCTGCAGGCCCGTCCCAGCTACTCTGCCAGCCTGGCATCCTCTCCTGTACGGGCTTCTCCGATGTTTGGAGTTGATGCATCTAGTGCAGCTGCTGTTTTTAATTCAAGGTCTGCTGCATTTGCAAAGAGGAGTCAGAGCTTCATTGAGCGTAGTGCTGGCAACCGTAATTCTGTGGTTTCTTCTTCTGCTGATTTTGGAACAATAAAGCCCTCTAACCTTTCAGATTGGGGCTCGCCTGGTGGCAAATTAGACTGGGGTATCCAGGGCGAGGAGCTGAACAAGCTGAGGAAATCTGCTTCTTTTGGATTCCGAAGCAGCGGGAGCAGTTCCCCAACAGCTTCATCCATGATGCCAACAAATGGTGTTGAGCCTGATGTATCATGGGTTCAATCTCTTGTCAAGGATGGACCTCAAGCCTCGCAACAACGCAGGCAATTTGGGTTCGAGGACCAGCAGCAGCAGCAGTGTCACCCTAACAACGGAGGTCCAGAGATGCTCCCCGCTTGGGTGGAGCAGCTTTACTTTGAGCAGGAGCAGATGGTGGCTTAA

Protein Analysis

629

Amino Acids

68.25

Weight (kDa)

6.35

Isoelectric Point (pI)

61.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_AtC3H23 PF25512 154 - 187 8.4e-21 AtC3H23-like, CCCH zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011766)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58620
fragaria_vesca FvH4_3g38750 FvH4_3g38750 FvH4_3g38750
malus_domestica MD03G1068400.v1.1 MD11G1072800.v1.1
prunus_persica Prupe.6G056300_v2.0.a1
pyrus_communis pycom03g05490 pycom11g06050
rosa_chinensis RchiOBHm_Chr5g0069901
rosa_laevigata RLG00000036106
rosa_multiflora Rmu_ssc0000133.1_g000002
rosa_roxburghii Rroxscaffold_1G00011160
rosa_rugosa Rorug05G0400600
rosa_samantha Rh5AG457700 Rh5BG476500 Rh5CG500300 Rh5DG488400
rosa_wichuraiana Rw5G042740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 3 cut(s) 235, 629, 1347
AccB7I CCANNNNNTGG 1 cut(s) 1697
AccI GTMKAC 1 cut(s) 473
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 7 cut(s) 9, 81, 559, 857, 905, 1656, 1839
AclWI GGATC 6 cut(s) 560, 1077, 1235, 1240, 1309, 1322
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 229
AfaI GTAC 3 cut(s) 689, 694, 1386
AfiI CCNNNNNNNGG 9 cut(s) 544, 941, 1066, 1276, 1403, 1606, 1697, 1817, 1843
AflIII ACRYGT 2 cut(s) 30, 948
AhdI GACNNNNNGTC 2 cut(s) 525, 844
AjnI CCWGG 6 cut(s) 208, 994, 1059, 1368, 1573, 1599
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 2 cut(s) 329, 707
AlwI GGATC 6 cut(s) 560, 1077, 1235, 1240, 1309, 1322
AlwNI CAGNNNCTG 3 cut(s) 791, 824, 1430
Ama87I CYCGRG 1 cut(s) 541
Aor13HI TCCGGA 1 cut(s) 427
AoxI GGCC 3 cut(s) 501, 1267, 1344
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
AseI ATTAAT 1 cut(s) 330
AspLEI GCGC 1 cut(s) 1326
AspS9I GGNCC 7 cut(s) 501, 618, 923, 1345, 1745, 1786, 1823
AsuC2I CCSGG 3 cut(s) 542, 543, 602
AsuHPI GGTGA 3 cut(s) 224, 668, 1799
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 5 cut(s) 618, 923, 1745, 1786, 1823
BaeI ACNNNNGTAYC 2 cut(s) 1579, 1612
BamHI GGATCC 1 cut(s) 1314
BanII GRGCYC 1 cut(s) 1571
BauI CACGAG 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 497
BbvCI CCTCAGC 2 cut(s) 141, 1622
BccI CCATC 5 cut(s) 380, 883, 1123, 1736, 1870
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 6 cut(s) 210, 996, 1061, 1370, 1575, 1601
BciVI GTATCC 2 cut(s) 417, 1607
BcnI CCSGG 3 cut(s) 542, 543, 602
BcoDI GTCTC 2 cut(s) 329, 707
BfaI CTAG 4 cut(s) 807, 1026, 1119, 1419
BfmI CTRYAG 2 cut(s) 622, 1340
BfuAI ACCTGC 3 cut(s) 235, 629, 1347
BfuI GTATCC 2 cut(s) 417, 1607
BglI GCCNNNNNGGC 1 cut(s) 1370
BlpI GCTNAGC 1 cut(s) 1055
Bme1390I CCNGG 9 cut(s) 210, 542, 543, 602, 996, 1061, 1370, 1575, 1601
Bme18I GGWCC 5 cut(s) 618, 923, 1745, 1786, 1823
BmeRI GACNNNNNGTC 2 cut(s) 525, 844
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 7 cut(s) 501, 618, 923, 1345, 1745, 1786, 1823
BmiI GGNNCC 3 cut(s) 619, 1111, 1316
BmrFI CCNGG 9 cut(s) 210, 542, 543, 602, 996, 1061, 1370, 1575, 1601
BmrI ACTGGG 5 cut(s) 285, 679, 1288, 1320, 1600
BmuI ACTGGG 5 cut(s) 285, 679, 1288, 1320, 1600
BpmI CTGGAG 1 cut(s) 1082
Bpu10I CCTNAGC 2 cut(s) 141, 1622
Bpu1102I GCTNAGC 1 cut(s) 1055
BpuEI CTTGAG 3 cut(s) 263, 481, 1734
BpuMI CCSGG 3 cut(s) 542, 543, 602
BsaBI GATNNNNATC 1 cut(s) 1009
BsaJI CCNNGG 4 cut(s) 541, 542, 569, 1600
BsaWI WCCGGW 2 cut(s) 427, 1106
Bsc4I CCNNNNNNNGG 9 cut(s) 544, 941, 1066, 1276, 1403, 1606, 1697, 1817, 1843
Bse1I ACTGG 7 cut(s) 291, 521, 685, 925, 1294, 1315, 1595
Bse8I GATNNNNATC 1 cut(s) 1009
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 6 cut(s) 210, 996, 1061, 1370, 1575, 1601
BseDI CCNNGG 4 cut(s) 541, 542, 569, 1600
BseJI GATNNNNATC 1 cut(s) 1009
BseLI CCNNNNNNNGG 9 cut(s) 544, 941, 1066, 1276, 1403, 1606, 1697, 1817, 1843
BseMII CTCAG 4 cut(s) 132, 831, 1010, 1613
BseNI ACTGG 7 cut(s) 291, 521, 685, 925, 1294, 1315, 1595
BseRI GAGGAG 5 cut(s) 360, 773, 1212, 1480, 1622
BseYI CCCAGC 1 cut(s) 1352
BsgI GTGCAG 1 cut(s) 1443
BshFI GGCC 3 cut(s) 503, 1269, 1346
BsiHKAI GWGCWC 1 cut(s) 497
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 4 cut(s) 428, 542, 602, 1107
BslFI GGGAC 2 cut(s) 631, 1335
BslI CCNNNNNNNGG 9 cut(s) 544, 941, 1066, 1276, 1403, 1606, 1697, 1817, 1843
BsmAI GTCTC 2 cut(s) 329, 707
BsmFI GGGAC 2 cut(s) 631, 1335
BsnI GGCC 3 cut(s) 503, 1269, 1346
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 2 cut(s) 497, 1571
Bsp13I TCCGGA 1 cut(s) 427
Bsp1720I GCTNAGC 1 cut(s) 1055
BspACI CCGC 7 cut(s) 9, 81, 559, 857, 905, 1656, 1839
BspANI GGCC 3 cut(s) 503, 1269, 1346
BspCNI CTCAG 4 cut(s) 133, 830, 1011, 1614
BspEI TCCGGA 1 cut(s) 427
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 619, 1111, 1316
BspMAI CTGCAG 2 cut(s) 626, 1344
BspMI ACCTGC 3 cut(s) 235, 629, 1347
BspPI GGATC 6 cut(s) 560, 1077, 1235, 1240, 1309, 1322
BspQI GCTCTTC 1 cut(s) 108
BsrI ACTGG 7 cut(s) 291, 521, 685, 925, 1294, 1315, 1595
BssECI CCNNGG 4 cut(s) 541, 542, 569, 1600
BssNAI GTATAC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 569
Bst1107I GTATAC 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 6 cut(s) 210, 996, 1061, 1370, 1575, 1601
Bst4CI ACNGT 2 cut(s) 692, 1502
Bst6I CTCTTC 2 cut(s) 108, 429
BstAPI GCANNNNNTGC 1 cut(s) 1421
BstC8I GCNNGC 8 cut(s) 79, 162, 626, 1344, 1366, 1495, 1571, 1769
BstDEI CTNAG 5 cut(s) 141, 817, 1019, 1055, 1622
BstHHI GCGC 1 cut(s) 1326
BstMAI GTCTC 2 cut(s) 329, 707
BstNI CCWGG 6 cut(s) 210, 996, 1061, 1370, 1575, 1601
BstNSI RCATGY 1 cut(s) 952
BstSCI CCNGG 9 cut(s) 208, 540, 541, 600, 994, 1059, 1368, 1573, 1599
BstSFI CTRYAG 2 cut(s) 622, 1340
BstX2I RGATCY 2 cut(s) 1232, 1314
BstXI CCANNNNNNTGG 1 cut(s) 1122
BstYI RGATCY 2 cut(s) 1232, 1314
BstZ17I GTATAC 1 cut(s) 474
BsuI GTATCC 2 cut(s) 417, 1607
BsuRI GGCC 3 cut(s) 503, 1269, 1346
BtsI GCAGTG 2 cut(s) 822, 1808
BtsIMutI CAGTG 3 cut(s) 822, 918, 1808
BveI ACCTGC 3 cut(s) 235, 629, 1347
Cac8I GCNNGC 8 cut(s) 79, 162, 626, 1344, 1366, 1495, 1571, 1769
CaiI CAGNNNCTG 3 cut(s) 791, 824, 1430
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 1326
Cfr13I GGNCC 7 cut(s) 501, 618, 923, 1345, 1745, 1786, 1823
Cfr9I CCCGGG 1 cut(s) 541
CsiI ACCWGGT 1 cut(s) 208
Csp6I GTAC 3 cut(s) 688, 693, 1385
CviQI GTAC 3 cut(s) 688, 693, 1385
DdeI CTNAG 5 cut(s) 141, 817, 1019, 1055, 1622
DriI GACNNNNNGTC 2 cut(s) 525, 844
Eam1104I CTCTTC 2 cut(s) 108, 429
Eam1105I GACNNNNNGTC 2 cut(s) 525, 844
EarI CTCTTC 2 cut(s) 108, 429
EciI GGCGGA 1 cut(s) 894
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 1571
Eco32I GATATC 1 cut(s) 1104
Eco47I GGWCC 5 cut(s) 618, 923, 1745, 1786, 1823
Eco57I CTGAAG 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 541
EcoO109I RGGNCCY 2 cut(s) 501, 618
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 6 cut(s) 208, 994, 1059, 1368, 1573, 1599
EcoRV GATATC 1 cut(s) 1104
EcoT14I CCWWGG 1 cut(s) 569
EcoT22I ATGCAT 1 cut(s) 1417
EcoT38I GRGCYC 1 cut(s) 1571
ErhI CCWWGG 1 cut(s) 569
FaqI GGGAC 2 cut(s) 631, 1335
FauI CCCGC 3 cut(s) 864, 1649, 1846
FblI GTMKAC 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 1571
FspBI CTAG 4 cut(s) 807, 1026, 1119, 1419
GlaI GCGC 1 cut(s) 1325
GsaI CCCAGC 1 cut(s) 1356
GsuI CTGGAG 1 cut(s) 1082
HaeIII GGCC 3 cut(s) 503, 1269, 1346
HapII CCGG 4 cut(s) 428, 542, 602, 1107
HhaI GCGC 1 cut(s) 1326
Hin6I GCGC 1 cut(s) 1324
HinP1I GCGC 1 cut(s) 1324
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 5 cut(s) 886, 913, 1168, 1468, 1644
HpaII CCGG 4 cut(s) 428, 542, 602, 1107
HphI GGTGA 3 cut(s) 224, 668, 1799
Hpy166II GTNNAC 3 cut(s) 474, 538, 1204
Hpy188I TCNGA 8 cut(s) 88, 319, 611, 1020, 1398, 1473, 1561, 1649
Hpy188III TCNNGA 9 cut(s) 188, 428, 436, 512, 836, 1048, 1238, 1318, 1826
Hpy8I GTNNAC 3 cut(s) 474, 538, 1204
Hpy99I CGWCG 1 cut(s) 1077
HpyAV CCTTC 5 cut(s) 335, 723, 810, 936, 1071
HpyCH4III ACNGT 2 cut(s) 692, 1502
HpyCH4IV ACGT 1 cut(s) 30
HpyF3I CTNAG 5 cut(s) 141, 817, 1019, 1055, 1622
HpySE526I ACGT 1 cut(s) 30
HspAI GCGC 1 cut(s) 1324
Kpn2I TCCGGA 1 cut(s) 427
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 7 cut(s) 319, 760, 1609, 1659, 1839, 1849, 1870
MabI ACCWGGT 1 cut(s) 208
MaeI CTAG 4 cut(s) 807, 1026, 1119, 1419
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 3 cut(s) 212, 1296, 1805
MboII GAAGA 8 cut(s) 38, 95, 268, 409, 416, 921, 1509, 1512
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 2 cut(s) 1232, 1314
MhlI GDGCHC 2 cut(s) 497, 1571
MlyI GAGTC 2 cut(s) 907, 1477
MmeI TCCRAC 1 cut(s) 1331
Mph1103I ATGCAT 1 cut(s) 1417
MroI TCCGGA 1 cut(s) 427
MseI TTAA 5 cut(s) 69, 330, 1263, 1437, 1885
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 3 cut(s) 588, 1427, 1656
MspI CCGG 4 cut(s) 428, 542, 602, 1107
MspR9I CCNGG 9 cut(s) 210, 542, 543, 602, 996, 1061, 1370, 1575, 1601
MunI CAATTG 1 cut(s) 849
MvaI CCWGG 6 cut(s) 210, 996, 1061, 1370, 1575, 1601
NciI CCSGG 3 cut(s) 542, 543, 602
NlaIV GGNNCC 3 cut(s) 619, 1111, 1316
NmuCI GTSAC 2 cut(s) 212, 1805
NsiI ATGCAT 1 cut(s) 1417
NspI RCATGY 1 cut(s) 952
PagI TCATGA 1 cut(s) 511
PaqCI CACCTGC 1 cut(s) 235
PciI ACATGT 1 cut(s) 948
PciSI GCTCTTC 1 cut(s) 108
PfeI GAWTC 3 cut(s) 886, 1168, 1644
PflMI CCANNNNNTGG 1 cut(s) 1697
PleI GAGTC 2 cut(s) 907, 1476
PpsI GAGTC 2 cut(s) 907, 1476
PpuMI RGGWCCY 1 cut(s) 618
PscI ACATGT 1 cut(s) 948
PshBI ATTAAT 1 cut(s) 330
Psp5II RGGWCCY 1 cut(s) 618
Psp6I CCWGG 6 cut(s) 208, 994, 1059, 1368, 1573, 1599
PspFI CCCAGC 1 cut(s) 1352
PspGI CCWGG 6 cut(s) 208, 994, 1059, 1368, 1573, 1599
PspN4I GGNNCC 3 cut(s) 619, 1111, 1316
PspPI GGNCC 7 cut(s) 501, 618, 923, 1345, 1745, 1786, 1823
PspPPI RGGWCCY 1 cut(s) 618
PsrI GAACNNNNNNTAC 2 cut(s) 1707, 1739
PstI CTGCAG 2 cut(s) 626, 1344
PstNI CAGNNNCTG 3 cut(s) 791, 824, 1430
PsuI RGATCY 2 cut(s) 1232, 1314
PvuII CAGCTG 2 cut(s) 588, 1427
RsaI GTAC 3 cut(s) 689, 694, 1386
RsaNI GTAC 3 cut(s) 688, 693, 1385
RseI CAYNNNNRTG 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 5 cut(s) 69, 330, 1263, 1437, 1885
Sau96I GGNCC 7 cut(s) 501, 618, 923, 1345, 1745, 1786, 1823
SbfI CCTGCAGG 2 cut(s) 626, 1344
SchI GAGTC 2 cut(s) 907, 1477
ScrFI CCNGG 9 cut(s) 210, 542, 543, 602, 996, 1061, 1370, 1575, 1601
SdaI CCTGCAGG 2 cut(s) 626, 1344
SduI GDGCHC 2 cut(s) 497, 1571
SexAI ACCWGGT 1 cut(s) 208
SfcI CTRYAG 2 cut(s) 622, 1340
SinI GGWCC 5 cut(s) 618, 923, 1745, 1786, 1823
SmaI CCCGGG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 3 cut(s) 278, 496, 1749
SmoI CTYRAG 3 cut(s) 278, 496, 1749
Sse8387I CCTGCAGG 2 cut(s) 626, 1344
SsiI CCGC 7 cut(s) 9, 81, 559, 857, 905, 1656, 1839
SspMI CTAG 4 cut(s) 807, 1026, 1119, 1419
StyD4I CCNGG 9 cut(s) 208, 540, 541, 600, 994, 1059, 1368, 1573, 1599
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 2 cut(s) 692, 1502
TaiI ACGT 1 cut(s) 33
TaqI TCGA 6 cut(s) 239, 251, 782, 1075, 1171, 1782
TauI GCSGC 1 cut(s) 562
TfiI GAWTC 3 cut(s) 886, 1168, 1644
Tru1I TTAA 5 cut(s) 69, 330, 1263, 1437, 1885
Tru9I TTAA 5 cut(s) 69, 330, 1263, 1437, 1885
TscAI CASTG 3 cut(s) 829, 925, 1808
TseFI GTSAC 2 cut(s) 212, 1805
Tsp45I GTSAC 2 cut(s) 212, 1805
TspDTI ATGAA 6 cut(s) 41, 95, 315, 1349, 1468, 1668
TspGWI ACGGA 2 cut(s) 470, 1833
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 3 cut(s) 829, 925, 1808
Van91I CCANNNNNTGG 1 cut(s) 1697
VpaK11BI GGWCC 5 cut(s) 618, 923, 1745, 1786, 1823
VspI ATTAAT 1 cut(s) 330
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 952
XmaI CCCGGG 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 4 cut(s) 807, 1026, 1119, 1419
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.