pycom11g11010

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006465
Physical Location & Seq
Forward (+)
9482714 .. 9489153
6440 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2643 bp
ATGAGGATTTTAGTGGTGGTTTTGGTGCTCGGAGTTCTGGCGACGGCGTCGTATTGCGCGAAGGTGACGTACGACCACAGAGCGCTGGTGATTGACGGGAAGCGCAGGGTTTTGGTGTCCGGCTCCATTCACTATCCTCGCAGCACCCCCGAGATGTGGCCGGACCTTATTCAGAAATCGAAAGACGGAGGCTTGGACGTGATCGAGACCTACGTCTTCTGGAACTTGCACGAAGCAGTTCGAGGCCAGTACGATTTTGGGGGAAGAAAAGATTTGGTTAAATTTGTGAAGACAGTAGCAGAAGCTGGCCTTTATGTGCACCTTAGAATTGGTCCCTATGTGTGCGCAGAATGGAACTACGGTGGATTTCCTCTTTGGTTGCATTTCATACCCGGAATTCAATTGCGAACGGACAATGAGCCGTTCAAGGCAGAAATGCAGCGATTCACGGCAAAAATTGTTGATATGATGAAGAAGGAGAAGCTTTATGCATCTCAGGGCGGACCGATCATTTTATCTCAGATTGAAAACGAGTATGGAAACATTGATCGAGCATACGGGGCAGCTGCCCAAACCTATATTAAGTGGGCAGCAGACATGGCTGTGTCTTTGGATACGGGGGTGCCTTGGGTCATGTGCCAGCAAGACGATGCTCCTCCATCTGTGATCAGCACTTGCAATGGATTTTATTGTGACCAATGGACCCCAAGACTTCCTGAAAAGAGACCCAAAATGTGGACTGAGAATTGGAGTGGATGGTTTCTTTCCTTTGGTGGTGCTGTGCCTCAGAGACCAGTGGAAGACCTTGCATTTGCCGTAGCACGCTTCTTTCAGCGAGGTGGAACTTTCCAAAATTATTATATGTACCATGGCGGGACTAACTTTGGGCGGAGTACTGGAGGACCCTTTATTGCTACCAGCTATGACTATGATGCTCCAATTGATGAGTATGGACTTCTTAGACAGCCTAAGTGGGGTCACCTAAAAGATGTGCATAAGGCCATAAAGCTTTGCGAAGAAGCCATGGTGGCCACTGACCCAAAATATTCTTCTTTTGGTCCAAACGTTGAGGCAACTGTTTACAAAACAGGATCAGCATGTGCTGCGTTCCTTGCTAATTCTGACACCAAATCTGATGCCACTGTGACTTTCAATGGAAATTCTTATCACTTGCCTGCATGGTCTGTGAGCATCTTACCAGACTGCAAAAATGTAGTGCTTAACACTGCAAAGATTAACTCTGCCGCCATGATTCCAAGCTTCATGCATCACTCGGTGATAGATGATATCGATTCCTCTGAAGCTTTGGGCTCAGGCTGGAGTTGGATAAATGAACCAGTGGGTATCTCAAAGAAGGATGCATTTGCCAGAGTTGGATTGTTGGAGCAAATAAACACCACAGCTGACAAAAGTGACTATTTGTGGTACTCTTTAAGCATTGATGTCACAAGTTCTGACACATTCCTTCAAGATGGATCTCAATCCATTCTTCATGTGGAATCACTTGGCCATGCCCTTCATGCGTTTATTAACGGAAAGCCTTCAGGGAGAGGAATCATCACTGCTAACAATGGCAAAATTTCTGTGGATATCCCTGTCACATTTGCATCTGGGAAGAACACAATAGATCTTCTGAGTTTGACTATTGGCCTTCAGGTTTCACCTTATTTCTTATTATATTCTGGAAGTGCAGTTTTTGTAAATGAATGCCATGCTTTAAGGTCAATTTCTGACCACGGATTGTTTTTTGTACATCAATTTTTCAATCAGAACTATGGAGCCTTCTTTGACAAATCAGGTGCCGGAATAACTGGTCCTGTTCAGCTGAAGGGCTTGAAAAATGGCACTACCACCGATCTCTCATCTCAGCGGTGGACGTATCAGATTGGACTTCAAGGGGAAGACTCGGGTTTTTCCAGCGGAAGTTCTTCTCAGTGGATTTCACAACCTACCTTACCCAAGAAACAACCCTTGACATGGTACAAGGCAACATTTAATGCTCCTGATGGCAGTAACCCTGTTGCACTAGACTTTACAGGGATGGGAAAGGGTGAAGCATGGGTGAACGGGCAAAGCATTGGGCGGTACTGGCCGACCAACAATGCTCCAACTAGCGGTTGCCCTGACTCTTGCAATTTCAGAGGACCTTATGACTCCAACAAATGCAGAAAGAACTGTGGAAAGCCATCTCAGGAATTGTACCACGTGCCTCGTTCATGGTTGAAACCAAGTGGCAATACTCTTGTCCTGTTTGAGGAAATTGGAGGCGATCCAACGCAGATATCTTTTGCTACAAGACAGATTGAAAGTTTGTGCTCACATGTATCAGAGTCTCACCCGTCGCCTGTAGATACGTGGAGTTCAGATTCAAAAGCGGGAAGGAAGTTAGGGCCTGTGCTATCACTAGAGTGCCCATTTCCTAACCAAGTCATTTCTTCAATCAAATTTGCAAGTTATGGAAAACCTCAGGGGACCTGTGGAAGCTTTAGCCATGGCCAGTGCAAGAGCACTAGCGCTCTATCCATTGTACAGAAGGCTTGCGTTGGATCCAAGAGTTGTAGCATTGAAGTCTCGGTTAAAACGTTCGGTGACCCATGTAAAGGAGTAGCAAAGAGTTTAGCAGTTGAAGCATCCTGTAGATAG

Protein Analysis

881

Amino Acids

96.37

Weight (kDa)

7.5

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 29 - 334 1.9e-122 Glycosyl hydrolases family 35
GHD PF17834 357 - 412 1.8e-24 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 658 - 746 3.6e-26 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 802 - 879 6.2e-21 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 622, 1799
AccB7I CCANNNNNTGG 1 cut(s) 735
AccII CGCG 1 cut(s) 59
AciI CCGC 9 cut(s) 501, 873, 889, 1245, 1870, 1920, 2083, 2115, 2375
AclI AACGTT 2 cut(s) 1065, 2582
AclWI GGATC 5 cut(s) 1099, 1483, 2264, 2541, 2554
AcoI YGGCCR 5 cut(s) 158, 1029, 1507, 2090, 2494
AcsI RAATTY 5 cut(s) 281, 396, 1159, 1578, 2444
AcuI CTGAAG 4 cut(s) 1320, 1527, 1637, 1847
AcvI CACGTG 1 cut(s) 2206
AcyI GRCGYC 1 cut(s) 47
AdeI CACNNNGTG 1 cut(s) 1276
AfeI AGCGCT 2 cut(s) 84, 2515
AfiI CCNNNNNNNGG 7 cut(s) 156, 735, 974, 1977, 2114, 2254, 2600
AflIII ACRYGT 1 cut(s) 2320
AjiI CACGTC 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 1033, 1065
AleI CACNNNNGTG 1 cut(s) 2407
Alw21I GWGCWC 4 cut(s) 30, 321, 2318, 2510
Alw26I GTCTC 5 cut(s) 200, 718, 784, 2337, 2575
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 5 cut(s) 1099, 1483, 2264, 2541, 2554
AlwNI CAGNNNCTG 1 cut(s) 305
Ama87I CYCGRG 2 cut(s) 149, 1906
Aor51HI AGCGCT 2 cut(s) 84, 2515
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 6 cut(s) 141, 439, 563, 566, 590, 1103
ApoI RAATTY 5 cut(s) 281, 396, 1159, 1578, 2444
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
Asp700I GAANNNNTTC 3 cut(s) 237, 1540, 1927
AspLEI GCGC 5 cut(s) 59, 85, 105, 347, 2516
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 9 cut(s) 76, 100, 971, 1288, 1653, 2063, 2074, 2327, 2600
AvaI CYCGRG 2 cut(s) 149, 1906
AvaII GGWCC 9 cut(s) 163, 332, 503, 702, 902, 1058, 1814, 2144, 2472
AxyI CCTNAGG 1 cut(s) 2466
BaeGI GKGCMC 2 cut(s) 321, 2414
BaeI ACNNNNGTAYC 2 cut(s) 606, 639
BalI TGGCCA 3 cut(s) 1031, 1509, 2496
BamHI GGATCC 1 cut(s) 2546
BanI GGYRCC 2 cut(s) 622, 1799
BanII GRGCYC 1 cut(s) 1313
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbrPI CACGTG 1 cut(s) 2206
BbsI GAAGAC 4 cut(s) 208, 296, 807, 1908
Bbv12I GWGCWC 4 cut(s) 30, 321, 2318, 2510
BbvI GCAGC 6 cut(s) 153, 451, 553, 575, 602, 1090
BccI CCATC 6 cut(s) 667, 750, 1466, 2000, 2035, 2194
BceAI ACGGC 4 cut(s) 60, 406, 465, 800
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 5 cut(s) 200, 718, 784, 2337, 2575
BfaI CTAG 4 cut(s) 2027, 2112, 2405, 2511
BfmI CTRYAG 2 cut(s) 2346, 2635
BfoI RGCGCY 2 cut(s) 86, 2517
BfuI GTATCC 1 cut(s) 607
BglI GCCNNNNNGGC 1 cut(s) 1028
BglII AGATCT 1 cut(s) 1627
BisI GCNGC 7 cut(s) 142, 440, 564, 567, 591, 1104, 1245
BlsI GCNGC 7 cut(s) 143, 441, 565, 568, 592, 1105, 1246
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 9 cut(s) 163, 332, 503, 702, 902, 1058, 1814, 2144, 2472
BmeT110I CYCGRG 2 cut(s) 149, 1906
BmgBI CACGTC 1 cut(s) 199
BmiI GGNNCC 9 cut(s) 124, 334, 624, 704, 904, 1780, 1801, 2473, 2548
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 9 cut(s) 500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2639
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 4 cut(s) 208, 296, 807, 1908
BpmI CTGGAG 2 cut(s) 918, 1339
Bpu10I CCTNAGC 1 cut(s) 1312
BpuMI CCSGG 1 cut(s) 393
Bsa29I ATCGAT 1 cut(s) 1290
BsaAI YACGTR 2 cut(s) 2206, 2355
BsaBI GATNNNNATC 1 cut(s) 1480
BsaHI GRCGYC 1 cut(s) 47
BsaI GGTCTC 3 cut(s) 200, 718, 784
BsaJI CCNNGG 5 cut(s) 626, 868, 1023, 1735, 2491
Bsc4I CCNNNNNNNGG 7 cut(s) 156, 735, 974, 1977, 2114, 2254, 2600
Bse1I ACTGG 7 cut(s) 247, 794, 901, 1337, 1816, 2093, 2497
Bse21I CCTNAGG 1 cut(s) 2466
Bse3DI GCAATG 1 cut(s) 685
Bse8I GATNNNNATC 1 cut(s) 1480
BseCI ATCGAT 1 cut(s) 1290
BseDI CCNNGG 5 cut(s) 626, 868, 1023, 1735, 2491
BseGI GGATG 4 cut(s) 761, 1363, 2046, 2630
BseJI GATNNNNATC 1 cut(s) 1480
BseLI CCNNNNNNNGG 7 cut(s) 156, 735, 974, 1977, 2114, 2254, 2600
BseMI GCAATG 1 cut(s) 685
BseNI ACTGG 7 cut(s) 247, 794, 901, 1337, 1816, 2093, 2497
BseRI GAGGAG 1 cut(s) 645
BseSI GKGCMC 2 cut(s) 321, 2414
BseXI GCAGC 6 cut(s) 153, 451, 553, 575, 602, 1090
BsgI GTGCAG 1 cut(s) 1710
Bsh1236I CGCG 1 cut(s) 59
BshNI GGYRCC 2 cut(s) 622, 1799
BshVI ATCGAT 1 cut(s) 1290
BsiHKAI GWGCWC 4 cut(s) 30, 321, 2318, 2510
BsiHKCI CYCGRG 2 cut(s) 149, 1906
BsiSI CCGG 4 cut(s) 120, 161, 393, 1803
BsiWI CGTACG 1 cut(s) 69
BslFI GGGAC 3 cut(s) 318, 889, 2485
BslI CCNNNNNNNGG 7 cut(s) 156, 735, 974, 1977, 2114, 2254, 2600
BsmAI GTCTC 5 cut(s) 200, 718, 784, 2337, 2575
BsmFI GGGAC 3 cut(s) 318, 889, 2485
BsmI GAATGC 1 cut(s) 1712
Bso31I GGTCTC 3 cut(s) 200, 718, 784
BsoBI CYCGRG 2 cut(s) 149, 1906
Bsp1286I GDGCHC 6 cut(s) 30, 321, 1313, 2318, 2414, 2510
Bsp1407I TGTACA 2 cut(s) 1750, 2527
Bsp19I CCATGG 3 cut(s) 868, 1023, 2491
BspACI CCGC 9 cut(s) 501, 873, 889, 1245, 1870, 1920, 2083, 2115, 2375
BspDI ATCGAT 1 cut(s) 1290
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 9 cut(s) 124, 334, 624, 704, 904, 1780, 1801, 2473, 2548
BspPI GGATC 5 cut(s) 1099, 1483, 2264, 2541, 2554
BspT107I GGYRCC 2 cut(s) 622, 1799
BspTNI GGTCTC 3 cut(s) 200, 718, 784
BsrDI GCAATG 1 cut(s) 685
BsrGI TGTACA 2 cut(s) 1750, 2527
BsrI ACTGG 7 cut(s) 247, 794, 901, 1337, 1816, 2093, 2497
BssECI CCNNGG 5 cut(s) 626, 868, 1023, 1735, 2491
BssNI GRCGYC 1 cut(s) 47
BssT1I CCWWGG 4 cut(s) 626, 868, 1023, 2491
Bst4CI ACNGT 5 cut(s) 295, 362, 1078, 1144, 2177
BstACI GRCGYC 1 cut(s) 47
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 2 cut(s) 1750, 2527
BstBAI YACGTR 2 cut(s) 2206, 2355
BstC8I GCNNGC 5 cut(s) 307, 641, 823, 1176, 2539
BstDSI CCRYGG 4 cut(s) 868, 1023, 1735, 2491
BstEII GGTNACC 2 cut(s) 977, 2588
BstENI CCTNNNNNAGG 1 cut(s) 2252
BstF5I GGATG 4 cut(s) 761, 1363, 2046, 2630
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 2 cut(s) 86, 2517
BstHHI GCGC 5 cut(s) 59, 85, 105, 347, 2516
BstMAI GTCTC 5 cut(s) 200, 718, 784, 2337, 2575
BstMWI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1112, 1520, 2089, 2627
BstNSI RCATGY 2 cut(s) 1101, 2324
BstPAI GACNNNNGTC 1 cut(s) 212
BstPI GGTNACC 2 cut(s) 977, 2588
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 2 cut(s) 2346, 2635
BstSLI GKGCMC 2 cut(s) 321, 2414
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 6 cut(s) 153, 451, 553, 575, 602, 1090
BstV2I GAAGAC 4 cut(s) 208, 296, 807, 1908
BstX2I RGATCY 3 cut(s) 1475, 1627, 2546
BstYI RGATCY 3 cut(s) 1475, 1627, 2546
Bsu15I ATCGAT 1 cut(s) 1290
Bsu36I CCTNAGG 1 cut(s) 2466
BsuI GTATCC 1 cut(s) 607
BsuTUI ATCGAT 1 cut(s) 1290
BtgI CCRYGG 4 cut(s) 868, 1023, 1735, 2491
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 4 cut(s) 761, 1363, 2046, 2630
BtsI GCAGTG 2 cut(s) 1224, 1560
BtsIMutI CAGTG 8 cut(s) 801, 1032, 1140, 1224, 1344, 1560, 1940, 2504
Cac8I GCNNGC 5 cut(s) 307, 641, 823, 1176, 2539
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 5 cut(s) 59, 85, 105, 347, 2516
ClaI ATCGAT 1 cut(s) 1290
CpoI CGGWCCG 1 cut(s) 503
CseI GACGC 1 cut(s) 36
CspI CGGWCCG 1 cut(s) 503
DraIII CACNNNGTG 1 cut(s) 1276
EaeI YGGCCR 5 cut(s) 158, 1029, 1507, 2090, 2494
EciI GGCGGA 2 cut(s) 516, 904
Eco130I CCWWGG 4 cut(s) 626, 868, 1023, 2491
Eco24I GRGCYC 1 cut(s) 1313
Eco31I GGTCTC 3 cut(s) 200, 718, 784
Eco32I GATATC 3 cut(s) 1288, 1591, 2283
Eco47I GGWCC 9 cut(s) 163, 332, 503, 702, 902, 1058, 1814, 2144, 2472
Eco47III AGCGCT 2 cut(s) 84, 2515
Eco57I CTGAAG 4 cut(s) 1320, 1527, 1637, 1847
Eco72I CACGTG 1 cut(s) 2206
Eco81I CCTNAGG 1 cut(s) 2466
Eco88I CYCGRG 2 cut(s) 149, 1906
Eco91I GGTNACC 2 cut(s) 977, 2588
EcoNI CCTNNNNNAGG 1 cut(s) 2252
EcoO109I RGGNCCY 4 cut(s) 902, 2144, 2390, 2472
EcoO65I GGTNACC 2 cut(s) 977, 2588
EcoRI GAATTC 1 cut(s) 396
EcoRV GATATC 3 cut(s) 1288, 1591, 2283
EcoT14I CCWWGG 4 cut(s) 626, 868, 1023, 2491
EcoT22I ATGCAT 3 cut(s) 493, 1269, 1363
EcoT38I GRGCYC 1 cut(s) 1313
ErhI CCWWGG 4 cut(s) 626, 868, 1023, 2491
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 3 cut(s) 318, 889, 2485
FauI CCCGC 2 cut(s) 866, 2368
FbaI TGATCA 1 cut(s) 666
Fnu4HI GCNGC 7 cut(s) 142, 440, 564, 567, 591, 1104, 1245
FokI GGATG 4 cut(s) 768, 1370, 2053, 2617
FriOI GRGCYC 1 cut(s) 1313
Fsp4HI GCNGC 7 cut(s) 142, 440, 564, 567, 591, 1104, 1245
FspBI CTAG 4 cut(s) 2027, 2112, 2405, 2511
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 5 cut(s) 58, 84, 104, 346, 2515
GluI GCNGC 7 cut(s) 142, 440, 564, 567, 591, 1104, 1245
GsuI CTGGAG 2 cut(s) 918, 1339
HaeII RGCGCY 2 cut(s) 86, 2517
HapII CCGG 4 cut(s) 120, 161, 393, 1803
HgaI GACGC 1 cut(s) 36
HhaI GCGC 5 cut(s) 59, 85, 105, 347, 2516
Hin1I GRCGYC 1 cut(s) 47
Hin6I GCGC 5 cut(s) 57, 83, 103, 345, 2514
HinP1I GCGC 5 cut(s) 57, 83, 103, 345, 2514
HindIII AAGCTT 5 cut(s) 482, 1007, 1258, 1302, 2482
HpaII CCGG 4 cut(s) 120, 161, 393, 1803
HphI GGTGA 9 cut(s) 76, 100, 971, 1288, 1653, 2063, 2074, 2327, 2600
Hpy166II GTNNAC 5 cut(s) 319, 738, 1081, 1875, 2065
Hpy188III TCNNGA 7 cut(s) 205, 220, 716, 1469, 1683, 2003, 2192
Hpy8I GTNNAC 5 cut(s) 319, 738, 1081, 1875, 2065
Hpy99I CGWCG 3 cut(s) 46, 52, 2344
HpyCH4III ACNGT 5 cut(s) 295, 362, 1078, 1144, 2177
HpyCH4IV ACGT 8 cut(s) 68, 198, 213, 1065, 1877, 2205, 2354, 2582
HpyF10VI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1112, 1520, 2089, 2627
HpySE526I ACGT 8 cut(s) 68, 198, 213, 1065, 1877, 2205, 2354, 2582
Hsp92I GRCGYC 1 cut(s) 47
HspAI GCGC 5 cut(s) 57, 83, 103, 345, 2514
Ksp22I TGATCA 1 cut(s) 666
LmnI GCTCC 7 cut(s) 128, 658, 940, 1384, 1778, 2005, 2110
Lsp1109I GCAGC 6 cut(s) 153, 451, 553, 575, 602, 1090
LweI GCATC 9 cut(s) 500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2639
MaeI CTAG 4 cut(s) 2027, 2112, 2405, 2511
MaeII ACGT 8 cut(s) 68, 198, 213, 1065, 1877, 2205, 2354, 2582
MaeIII GTNAC 9 cut(s) 64, 692, 977, 1144, 1412, 1444, 1597, 2012, 2588
MfeI CAATTG 2 cut(s) 401, 939
MflI RGATCY 3 cut(s) 1475, 1627, 2546
MhlI GDGCHC 6 cut(s) 30, 321, 1313, 2318, 2414, 2510
MlsI TGGCCA 3 cut(s) 1031, 1509, 2496
MluNI TGGCCA 3 cut(s) 1031, 1509, 2496
MlyI GAGTC 4 cut(s) 1898, 2120, 2147, 2339
MmeI TCCRAC 7 cut(s) 1304, 1354, 1362, 2132, 2181, 2297, 2524
Mox20I TGGCCA 3 cut(s) 1031, 1509, 2496
Mph1103I ATGCAT 3 cut(s) 493, 1269, 1363
MroXI GAANNNNTTC 3 cut(s) 237, 1540, 1927
MscI TGGCCA 3 cut(s) 1031, 1509, 2496
MseI TTAA 9 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1718, 1995, 2577
MslI CAYNNNNRTG 2 cut(s) 602, 2407
Msp20I TGGCCA 3 cut(s) 1031, 1509, 2496
MspA1I CMGCKG 5 cut(s) 566, 1403, 1825, 1870, 1920
MspI CCGG 4 cut(s) 120, 161, 393, 1803
MspR9I CCNGG 1 cut(s) 393
MunI CAATTG 2 cut(s) 401, 939
Mva1269I GAATGC 1 cut(s) 1712
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1112, 1520, 2089, 2627
NciI CCSGG 1 cut(s) 393
NcoI CCATGG 3 cut(s) 868, 1023, 2491
NlaIV GGNNCC 9 cut(s) 124, 334, 624, 704, 904, 1780, 1801, 2473, 2548
NmuCI GTSAC 8 cut(s) 64, 692, 977, 1144, 1412, 1444, 1597, 2588
NsbI TGCGCA 1 cut(s) 346
NsiI ATGCAT 3 cut(s) 493, 1269, 1363
NspI RCATGY 2 cut(s) 1101, 2324
OliI CACNNNNGTG 1 cut(s) 2407
PciI ACATGT 1 cut(s) 2320
PcsI WCGNNNNNNNCGW 3 cut(s) 56, 210, 2579
PctI GAATGC 1 cut(s) 1712
PdmI GAANNNNTTC 3 cut(s) 237, 1540, 1927
PfeI GAWTC 6 cut(s) 444, 1252, 1292, 1499, 1554, 2366
Pfl23II CGTACG 1 cut(s) 69
PflFI GACNNNGTC 1 cut(s) 46
PflMI CCANNNNNTGG 1 cut(s) 735
PkrI GCNGC 7 cut(s) 143, 441, 565, 568, 592, 1105, 1246
PleI GAGTC 4 cut(s) 1898, 2120, 2147, 2338
PmaCI CACGTG 1 cut(s) 2206
PmlI CACGTG 1 cut(s) 2206
PpsI GAGTC 4 cut(s) 1898, 2120, 2147, 2338
Ppu21I YACGTR 2 cut(s) 2206, 2355
PpuMI RGGWCCY 3 cut(s) 902, 2144, 2472
PscI ACATGT 1 cut(s) 2320
PshAI GACNNNNGTC 1 cut(s) 212
Psp1406I AACGTT 2 cut(s) 1065, 2582
Psp5II RGGWCCY 3 cut(s) 902, 2144, 2472
PspCI CACGTG 1 cut(s) 2206
PspEI GGTNACC 2 cut(s) 977, 2588
PspLI CGTACG 1 cut(s) 69
PspN4I GGNNCC 9 cut(s) 124, 334, 624, 704, 904, 1780, 1801, 2473, 2548
PspPPI RGGWCCY 3 cut(s) 902, 2144, 2472
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 3 cut(s) 1475, 1627, 2546
PsyI GACNNNGTC 1 cut(s) 46
PvuII CAGCTG 3 cut(s) 566, 1403, 1825
RseI CAYNNNNRTG 2 cut(s) 602, 2407
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 9 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1718, 1995, 2577
SatI GCNGC 7 cut(s) 142, 440, 564, 567, 591, 1104, 1245
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 4 cut(s) 1898, 2120, 2147, 2339
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 6 cut(s) 30, 321, 1313, 2318, 2414, 2510
SfaNI GCATC 9 cut(s) 500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2639
SfcI CTRYAG 2 cut(s) 2346, 2635
SinI GGWCC 9 cut(s) 163, 332, 503, 702, 902, 1058, 1814, 2144, 2472
SmiMI CAYNNNNRTG 2 cut(s) 602, 2407
SsiI CCGC 9 cut(s) 501, 873, 889, 1245, 1870, 1920, 2083, 2115, 2375
SspI AATATT 1 cut(s) 1046
SspMI CTAG 4 cut(s) 2027, 2112, 2405, 2511
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 4 cut(s) 626, 868, 1023, 2491
TaaI ACNGT 5 cut(s) 295, 362, 1078, 1144, 2177
TaiI ACGT 8 cut(s) 71, 201, 216, 1068, 1880, 2208, 2357, 2585
TaqI TCGA 5 cut(s) 179, 204, 241, 550, 1290
TaqII GACCGA 1 cut(s) 520
TatI WGTACW 3 cut(s) 893, 1750, 2527
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 6 cut(s) 444, 1252, 1292, 1499, 1554, 2366
Tru1I TTAA 9 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1718, 1995, 2577
Tru9I TTAA 9 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1718, 1995, 2577
TscAI CASTG 8 cut(s) 801, 1039, 1147, 1231, 1344, 1567, 1940, 2504
TseFI GTSAC 8 cut(s) 64, 692, 977, 1144, 1412, 1444, 1597, 2588
TseI GCWGC 6 cut(s) 141, 439, 563, 566, 590, 1103
Tsp45I GTSAC 8 cut(s) 64, 692, 977, 1144, 1412, 1444, 1597, 2588
TspDTI ATGAA 8 cut(s) 376, 485, 1252, 1347, 1481, 1508, 1719, 2205
TspGWI ACGGA 4 cut(s) 201, 425, 1548, 1752
TspRI CASTG 8 cut(s) 801, 1039, 1147, 1231, 1344, 1567, 1940, 2504
Tth111I GACNNNGTC 1 cut(s) 46
Van91I CCANNNNNTGG 1 cut(s) 735
VneI GTGCAC 1 cut(s) 317
VpaK11BI GGWCC 9 cut(s) 163, 332, 503, 702, 902, 1058, 1814, 2144, 2472
XagI CCTNNNNNAGG 1 cut(s) 2252
XapI RAATTY 5 cut(s) 281, 396, 1159, 1578, 2444
XceI RCATGY 2 cut(s) 1101, 2324
XcmI CCANNNNNNNNNTGG 2 cut(s) 254, 1492
XmnI GAANNNNTTC 3 cut(s) 237, 1540, 1927
XspI CTAG 4 cut(s) 2027, 2112, 2405, 2511
ZrmI AGTACT 1 cut(s) 895
Zsp2I ATGCAT 3 cut(s) 493, 1269, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.