Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0002472

Physical Location & Seq

Genomic Coordinates Reverse (-)

10190064 .. 10192120

Gene Structure 2057 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g11530.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGGATTCCCCCATCAGTAAAGGCACAGGCTGCTTTACCTCTTGCATATTGAGGAGCAAACCCCGAGCTACACCTGGAGCAATTGTTCAATTTTCATATTTTGAACTGGAAAGTGCAACCAATAAGTTTTCAGATTCCAATCTAATAGGACTTGGTGGAAGCAGCTATGTCTATCGTGGCCAGCTCAATGATGGCGAAGTTGTGGCAGTTAAGCGTCTAAAAGCTTTGAAAGGGCCAGACATGGACTCTGTCTTTTTAACAGAGATTGAAATGTTAGCCAGACTTAATCATTTTAATGTGGTGCCTTTGCTTGGCTACTGCTTTGAAACCCATGGAAAAAATGTTGAGAGGTTATTGATATTTGAGTACATGGATAACGGTAACCTGAGAGATTGTTTGGATGGGGATGAAGGGAAAAACATGGATTGGGTCACTCGAGTTTCAATTGCCATTGGAGCTGCAAGGGGTTTGGAATATCTCCATGAAGCAGCTGCTCCAAGAATTCTGCATCGAGATGTCAAATCCACAAACATTCTTCTGGATGAAAATTGGCAAGCAAAAATAACTGATCTTGGTATGGCGAAACGTTTAAAAGCTGATGGTGTCCCTAGCACATCTAGTTCTCCAGCAAGAATGCAGGGGACTTTTGGTTATTTTGCACCGGAATATGCTATTGCTGGAAGAGCATCTCTTGAGTCAGACGTTTTCAGTTTTGGCGTAGTTCTTCTTGAGCTTATCACTGGTCGGAAGCCCATCCATAAATCAACCACTAAGGGAGAAGAAAGCCTTGTCATATGGGCTATGCCTCGTTTACTGGATAGTAGGCGAGTAATCACAGAGTTGGCGGATCCAGATTTAAATGGAAACTTCCCAGAAGAGGAGATGCAGATAATGGCTTACTTGGCAAAAGAATGTCTACTGTTGGATCCTGATGCTAGACCAAACATGAGTGAGGTTGTCCAAATCCTTTCAACTATTTCACCAGAGAGATCTAAAAGGAAAAACTTTTCTGTAAATCTTTTTCAGCACATAGGCATGGATGGCCATAAAGATGCTGAGGAGATATGGCGAGCGAAATCCAGTAAAGGTTCAGTTCGGTGTTCACTGCCACTGGACATTGACCGTAACCTATGTGCTGAAAGAGACACGCATTCTGTTTCAGATAATATGGAGAGATTGATCCTCTTGACTTCAAATGCCAGGAGTTGTCGTGCCAACGATATTGAGACAGTGGATTTAACTGAGCCCAGGTTGGAGTCTTTTTGCGTAACAAATGGTAAGCCCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009888 GO:0009987 GO:0010008 GO:0010087 GO:0010089 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0031090 GO:0031410 GO:0031982 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0097708 GO:0098588 GO:0098805 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

430

Amino Acids

47.66

Weight (kDa)

5.64

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28250 AT2G28250 AT2G28250 AT2G28250 AT2G28250 AT2G28250
fragaria_vesca	FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760 FvH4_3g31760
malus_domestica	MD03G1120900.v1.1 MD11G1139700.v1.1
prunus_persica	Prupe.6G104400_v2.0.a1
pyrus_communis	pycom11g11530
rosa_chinensis	RchiOBHm_Chr5g0058481
rosa_laevigata	RLG00000035227
rosa_multiflora	Rmu_sc0000367.1_g000133
rosa_roxburghii	Rroxscaffold_1G00022020
rosa_rugosa	Rorug05G0315400 Rorug05G0315400 Rorug05G0315400 Rorug05G0315500 Rorug05G0315600 Rorug05G0315700
rosa_samantha	Rh5AG381600 Rh5BG393200 Rh5CG417400 Rh5DG408600
rosa_wichuraiana	Rw5G035960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	301
AccI	GTMKAC	1 cut(s)	916
AciI	CCGC	1 cut(s)	845
AclI	AACGTT	1 cut(s)	586
AclWI	GGATC	5 cut(s)	842, 855, 920, 933, 1174
AcoI	YGGCCR	2 cut(s)	178, 1042
AcsI	RAATTY	1 cut(s)	501
AfaI	GTAC	1 cut(s)	368
AfiI	CCNNNNNNNGG	2 cut(s)	311, 877
AgsI	TTSAA	8 cut(s)	89, 104, 229, 269, 326, 444, 972, 1194
AjnI	CCWGG	3 cut(s)	73, 1199, 1247
AloI	GAACNNNNNNTCC	2 cut(s)	69, 101
AluBI	AGCT	8 cut(s)	68, 165, 184, 224, 458, 491, 596, 733
AluI	AGCT	8 cut(s)	68, 165, 184, 224, 458, 491, 596, 733
Alw26I	GTCTC	2 cut(s)	1137, 1220
AlwI	GGATC	5 cut(s)	842, 855, 920, 933, 1174
Ama87I	CYCGRG	2 cut(s)	63, 435
AoxI	GGCC	3 cut(s)	178, 233, 1042
ApeKI	GCWGC	5 cut(s)	30, 162, 458, 488, 491
ApoI	RAATTY	1 cut(s)	501
AspS9I	GGNCC	1 cut(s)	233
AsuHPI	GGTGA	1 cut(s)	972
AvaI	CYCGRG	2 cut(s)	63, 435
BalI	TGGCCA	2 cut(s)	180, 1044
BamHI	GGATCC	2 cut(s)	847, 925
BanI	GGYRCC	1 cut(s)	301
BanII	GRGCYC	1 cut(s)	1248
BbvCI	CCTCAGC	1 cut(s)	1056
BbvI	GCAGC	5 cut(s)	17, 174, 445, 478, 500
BccI	CCATC	6 cut(s)	20, 185, 395, 593, 761, 1034
BciT130I	CCWGG	3 cut(s)	75, 1201, 1249
BcoDI	GTCTC	2 cut(s)	1137, 1220
BfaI	CTAG	3 cut(s)	609, 618, 936
BglII	AGATCT	1 cut(s)	989
BisI	GCNGC	5 cut(s)	31, 163, 459, 489, 492
BlsI	GCNGC	5 cut(s)	32, 164, 460, 490, 493
Bme1390I	CCNGG	3 cut(s)	75, 1201, 1249
BmeT110I	CYCGRG	2 cut(s)	63, 435
BmgT120I	GGNCC	1 cut(s)	233
BmiI	GGNNCC	3 cut(s)	303, 849, 927
BmrFI	CCNGG	3 cut(s)	75, 1201, 1249
BmsI	GCATC	5 cut(s)	517, 695, 873, 922, 1042
BpmI	CTGGAG	2 cut(s)	96, 609
Bpu10I	CCTNAGC	1 cut(s)	1056
BpuEI	CTTGAG	2 cut(s)	713, 749
BsaBI	GATNNNNATC	1 cut(s)	138
BsaJI	CCNNGG	2 cut(s)	331, 1247
BsaWI	WCCGGW	1 cut(s)	661
BsaXI	ACNNNNNCTCC	4 cut(s)	69, 99, 1195, 1225
Bsc4I	CCNNNNNNNGG	2 cut(s)	311, 877
Bse1I	ACTGG	5 cut(s)	111, 745, 819, 1080, 1116
Bse8I	GATNNNNATC	1 cut(s)	138
BseBI	CCWGG	3 cut(s)	75, 1201, 1249
BseDI	CCNNGG	2 cut(s)	331, 1247
BseGI	GGATG	5 cut(s)	406, 412, 547, 753, 1045
BseJI	GATNNNNATC	1 cut(s)	138
BseLI	CCNNNNNNNGG	2 cut(s)	311, 877
BseMII	CTCAG	3 cut(s)	377, 1047, 1233
BseNI	ACTGG	5 cut(s)	111, 745, 819, 1080, 1116
BseRI	GAGGAG	3 cut(s)	67, 893, 1073
BseXI	GCAGC	5 cut(s)	17, 174, 445, 478, 500
BshFI	GGCC	3 cut(s)	180, 235, 1044
BshNI	GGYRCC	1 cut(s)	301
BsiHKCI	CYCGRG	2 cut(s)	63, 435
BsiSI	CCGG	1 cut(s)	662
BslFI	GGGAC	2 cut(s)	590, 655
BslI	CCNNNNNNNGG	2 cut(s)	311, 877
BsmAI	GTCTC	2 cut(s)	1137, 1220
BsmFI	GGGAC	2 cut(s)	590, 655
BsmI	GAATGC	2 cut(s)	639, 1150
BsnI	GGCC	3 cut(s)	180, 235, 1044
BsoBI	CYCGRG	2 cut(s)	63, 435
Bsp1286I	GDGCHC	1 cut(s)	1248
Bsp143I	GATC	5 cut(s)	568, 847, 925, 989, 1179
Bsp19I	CCATGG	1 cut(s)	331
BspACI	CCGC	1 cut(s)	845
BspANI	GGCC	3 cut(s)	180, 235, 1044
BspCNI	CTCAG	3 cut(s)	378, 1048, 1234
BspLI	GGNNCC	3 cut(s)	303, 849, 927
BspPI	GGATC	5 cut(s)	842, 855, 920, 933, 1174
BspQI	GCTCTTC	1 cut(s)	676
BspT107I	GGYRCC	1 cut(s)	301
BsrI	ACTGG	5 cut(s)	111, 745, 819, 1080, 1116
BssECI	CCNNGG	2 cut(s)	331, 1247
BssMI	GATC	5 cut(s)	568, 847, 925, 989, 1179
BssT1I	CCWWGG	1 cut(s)	331
Bst2UI	CCWGG	3 cut(s)	75, 1201, 1249
Bst4CI	ACNGT	4 cut(s)	380, 921, 1124, 1231
Bst6I	CTCTTC	2 cut(s)	676, 870
BstAPI	GCANNNNNTGC	1 cut(s)	30
BstC8I	GCNNGC	3 cut(s)	182, 555, 1071
BstDEI	CTNAG	4 cut(s)	386, 771, 1056, 1242
BstDSI	CCRYGG	1 cut(s)	331
BstEII	GGTNACC	1 cut(s)	380
BstF5I	GGATG	5 cut(s)	406, 412, 547, 753, 1045
BstKTI	GATC	5 cut(s)	571, 850, 928, 992, 1182
BstMAI	GTCTC	2 cut(s)	1137, 1220
BstMBI	GATC	5 cut(s)	568, 847, 925, 989, 1179
BstMWI	GCNNNNNNNGC	5 cut(s)	30, 455, 683, 902, 1041
BstNI	CCWGG	3 cut(s)	75, 1201, 1249
BstPI	GGTNACC	1 cut(s)	380
BstSCI	CCNGG	3 cut(s)	73, 1199, 1247
BstV1I	GCAGC	5 cut(s)	17, 174, 445, 478, 500
BstX2I	RGATCY	3 cut(s)	847, 925, 989
BstYI	RGATCY	3 cut(s)	847, 925, 989
BsuRI	GGCC	3 cut(s)	180, 235, 1044
BtgI	CCRYGG	1 cut(s)	331
BtsCI	GGATG	5 cut(s)	406, 412, 547, 753, 1045
BtsI	GCAGTG	1 cut(s)	1103
BtsIMutI	CAGTG	4 cut(s)	738, 1103, 1109, 1236
Cac8I	GCNNGC	3 cut(s)	182, 555, 1071
Cfr13I	GGNCC	1 cut(s)	233
CseI	GACGC	1 cut(s)	203
Csp6I	GTAC	1 cut(s)	367
CviAII	CATG	8 cut(s)	241, 332, 370, 421, 482, 946, 1036, 1287
CviQI	GTAC	1 cut(s)	367
DdeI	CTNAG	4 cut(s)	386, 771, 1056, 1242
DpnI	GATC	5 cut(s)	570, 849, 927, 991, 1181
DpnII	GATC	5 cut(s)	568, 847, 925, 989, 1179
DraI	TTTAAA	2 cut(s)	591, 858
EaeI	YGGCCR	2 cut(s)	178, 1042
Eam1104I	CTCTTC	2 cut(s)	676, 870
EarI	CTCTTC	2 cut(s)	676, 870
EciI	GGCGGA	1 cut(s)	860
Eco130I	CCWWGG	1 cut(s)	331
Eco24I	GRGCYC	1 cut(s)	1248
Eco88I	CYCGRG	2 cut(s)	63, 435
Eco91I	GGTNACC	1 cut(s)	380
EcoO65I	GGTNACC	1 cut(s)	380
EcoRI	GAATTC	1 cut(s)	501
EcoRII	CCWGG	3 cut(s)	73, 1199, 1247
EcoT14I	CCWWGG	1 cut(s)	331
EcoT38I	GRGCYC	1 cut(s)	1248
ErhI	CCWWGG	1 cut(s)	331
FaeI	CATG	8 cut(s)	244, 335, 373, 424, 485, 949, 1039, 1290
FalI	AAGNNNNNCTT	2 cut(s)	771, 803
FaqI	GGGAC	2 cut(s)	590, 655
FatI	CATG	8 cut(s)	240, 331, 369, 420, 481, 945, 1035, 1286
FauNDI	CATATG	1 cut(s)	794
FblI	GTMKAC	1 cut(s)	916
Fnu4HI	GCNGC	5 cut(s)	31, 163, 459, 489, 492
FokI	GGATG	5 cut(s)	413, 419, 554, 740, 1052
FriOI	GRGCYC	1 cut(s)	1248
Fsp4HI	GCNGC	5 cut(s)	31, 163, 459, 489, 492
FspBI	CTAG	3 cut(s)	609, 618, 936
GluI	GCNGC	5 cut(s)	31, 163, 459, 489, 492
GsuI	CTGGAG	2 cut(s)	96, 609
HaeIII	GGCC	3 cut(s)	180, 235, 1044
HapII	CCGG	1 cut(s)	662
HgaI	GACGC	1 cut(s)	203
Hin1II	CATG	8 cut(s)	244, 335, 373, 424, 485, 949, 1039, 1290
HindIII	AAGCTT	1 cut(s)	222
HinfI	GANTC	5 cut(s)	5, 134, 245, 695, 1256
HpaII	CCGG	1 cut(s)	662
HphI	GGTGA	1 cut(s)	972
Hpy166II	GTNNAC	3 cut(s)	812, 917, 1103
Hpy188I	TCNGA	4 cut(s)	133, 700, 747, 1162
Hpy188III	TCNNGA	7 cut(s)	512, 539, 692, 728, 851, 929, 1186
Hpy8I	GTNNAC	3 cut(s)	812, 917, 1103
HpyAV	CCTTC	1 cut(s)	404
HpyCH4III	ACNGT	4 cut(s)	380, 921, 1124, 1231
HpyCH4IV	ACGT	2 cut(s)	586, 702
HpyCH4V	TGCA	7 cut(s)	45, 116, 461, 508, 637, 659, 886
HpyF10VI	GCNNNNNNNGC	5 cut(s)	30, 455, 683, 902, 1041
HpyF3I	CTNAG	4 cut(s)	386, 771, 1056, 1242
HpySE526I	ACGT	2 cut(s)	586, 702
Hsp92II	CATG	8 cut(s)	244, 335, 373, 424, 485, 949, 1039, 1290
Kzo9I	GATC	5 cut(s)	568, 847, 925, 989, 1179
LguI	GCTCTTC	1 cut(s)	676
LmnI	GCTCC	4 cut(s)	54, 77, 455, 499
Lsp1109I	GCAGC	5 cut(s)	17, 174, 445, 478, 500
LweI	GCATC	5 cut(s)	517, 695, 873, 922, 1042
MaeI	CTAG	3 cut(s)	609, 618, 936
MaeII	ACGT	2 cut(s)	586, 702
MaeIII	GTNAC	4 cut(s)	380, 430, 1124, 1267
MalI	GATC	5 cut(s)	570, 849, 927, 991, 1181
MboI	GATC	5 cut(s)	568, 847, 925, 989, 1179
MboII	GAAGA	5 cut(s)	527, 693, 716, 791, 887
MfeI	CAATTG	2 cut(s)	81, 444
MflI	RGATCY	3 cut(s)	847, 925, 989
MhlI	GDGCHC	1 cut(s)	1248
MlsI	TGGCCA	2 cut(s)	180, 1044
MluCI	AATT	5 cut(s)	81, 89, 444, 501, 547
MluNI	TGGCCA	2 cut(s)	180, 1044
MlyI	GAGTC	3 cut(s)	239, 704, 1265
MmeI	TCCRAC	3 cut(s)	725, 903, 1233
MnlI	CCTC	8 cut(s)	45, 49, 342, 816, 871, 946, 1051, 1193
Mox20I	TGGCCA	2 cut(s)	180, 1044
MscI	TGGCCA	2 cut(s)	180, 1044
MseI	TTAA	7 cut(s)	210, 257, 285, 294, 590, 857, 1238
MslI	CAYNNNNRTG	4 cut(s)	294, 513, 1034, 1050
Msp20I	TGGCCA	2 cut(s)	180, 1044
MspA1I	CMGCKG	1 cut(s)	491
MspI	CCGG	1 cut(s)	662
MspR9I	CCNGG	3 cut(s)	75, 1201, 1249
MunI	CAATTG	2 cut(s)	81, 444
Mva1269I	GAATGC	2 cut(s)	639, 1150
MvaI	CCWGG	3 cut(s)	75, 1201, 1249
MwoI	GCNNNNNNNGC	5 cut(s)	30, 455, 683, 902, 1041
NcoI	CCATGG	1 cut(s)	331
NdeI	CATATG	1 cut(s)	794
NdeII	GATC	5 cut(s)	568, 847, 925, 989, 1179
NlaIII	CATG	8 cut(s)	244, 335, 373, 424, 485, 949, 1039, 1290
NlaIV	GGNNCC	3 cut(s)	303, 849, 927
NmuCI	GTSAC	1 cut(s)	430
PaeR7I	CTCGAG	1 cut(s)	435
PciSI	GCTCTTC	1 cut(s)	676
PctI	GAATGC	2 cut(s)	639, 1150
PfeI	GAWTC	2 cut(s)	5, 134
PflFI	GACNNNGTC	1 cut(s)	248
PkrI	GCNGC	5 cut(s)	32, 164, 460, 490, 493
PleI	GAGTC	3 cut(s)	239, 703, 1264
PpsI	GAGTC	3 cut(s)	239, 703, 1264
Psp1406I	AACGTT	1 cut(s)	586
Psp6I	CCWGG	3 cut(s)	73, 1199, 1247
PspEI	GGTNACC	1 cut(s)	380
PspGI	CCWGG	3 cut(s)	73, 1199, 1247
PspN4I	GGNNCC	3 cut(s)	303, 849, 927
PspPI	GGNCC	1 cut(s)	233
PspXI	VCTCGAGB	1 cut(s)	435
PsuI	RGATCY	3 cut(s)	847, 925, 989
PsyI	GACNNNGTC	1 cut(s)	248
PvuII	CAGCTG	1 cut(s)	491
RsaI	GTAC	1 cut(s)	368
RsaNI	GTAC	1 cut(s)	367
RseI	CAYNNNNRTG	4 cut(s)	294, 513, 1034, 1050
SapI	GCTCTTC	1 cut(s)	676
SaqAI	TTAA	7 cut(s)	210, 257, 285, 294, 590, 857, 1238
SatI	GCNGC	5 cut(s)	31, 163, 459, 489, 492
Sau3AI	GATC	5 cut(s)	568, 847, 925, 989, 1179
Sau96I	GGNCC	1 cut(s)	233
SchI	GAGTC	3 cut(s)	239, 704, 1265
ScrFI	CCNGG	3 cut(s)	75, 1201, 1249
SduI	GDGCHC	1 cut(s)	1248
SfaNI	GCATC	5 cut(s)	517, 695, 873, 922, 1042
Sfr274I	CTCGAG	1 cut(s)	435
SlaI	CTCGAG	1 cut(s)	435
SmiI	ATTTAAAT	1 cut(s)	858
SmiMI	CAYNNNNRTG	4 cut(s)	294, 513, 1034, 1050
SmlI	CTYRAG	3 cut(s)	435, 692, 728
SmoI	CTYRAG	3 cut(s)	435, 692, 728
Sse9I	AATT	5 cut(s)	81, 89, 444, 501, 547
SsiI	CCGC	1 cut(s)	845
SspMI	CTAG	3 cut(s)	609, 618, 936
StyD4I	CCNGG	3 cut(s)	73, 1199, 1247
StyI	CCWWGG	1 cut(s)	331
SwaI	ATTTAAAT	1 cut(s)	858
TaaI	ACNGT	4 cut(s)	380, 921, 1124, 1231
TaiI	ACGT	2 cut(s)	589, 705
TaqI	TCGA	2 cut(s)	436, 511
TasI	AATT	5 cut(s)	81, 89, 444, 501, 547
TatI	WGTACW	1 cut(s)	366
TfiI	GAWTC	2 cut(s)	5, 134
Tru1I	TTAA	7 cut(s)	210, 257, 285, 294, 590, 857, 1238
Tru9I	TTAA	7 cut(s)	210, 257, 285, 294, 590, 857, 1238
TscAI	CASTG	4 cut(s)	745, 1110, 1116, 1236
TseFI	GTSAC	1 cut(s)	430
TseI	GCWGC	5 cut(s)	30, 162, 458, 488, 491
Tsp45I	GTSAC	1 cut(s)	430
TspDTI	ATGAA	4 cut(s)	84, 423, 498, 558
TspRI	CASTG	4 cut(s)	745, 1110, 1116, 1236
Tth111I	GACNNNGTC	1 cut(s)	248
XapI	RAATTY	1 cut(s)	501
XcmI	CCANNNNNNNNNTGG	1 cut(s)	188
XhoI	CTCGAG	1 cut(s)	435
XmiI	GTMKAC	1 cut(s)	916
XspI	CTAG	3 cut(s)	609, 618, 936

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.