pycom11g11970

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014989
Physical Location & Seq
Forward (+)
10839893 .. 10841791
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGCATAACTTCCCCATCAACCAACTTATCACCTTTGCAATGTGGAGCCTACCTAACTTCAAAATTCTCATACCCCACCCCTCTGTTTTTCGCTGCATTTCCTTCTATCCTTTTCTTAATTCAATCTTCATGGCTCCTCTCATCTTCCTAATCCTTCTTTTCCTAATGATTTCTTCAAGCATTGCTCAGGGTCCCCCCTCACCTGGCTATAACCCTAGTTTTAAAATTAGCACTCTAGGGTTTGATCAGGGTTTTAGAACCCTTTGGGGTCCTCAACATCAAAATTTAGACCAGGGTACTTTAACACTCTGGCTTGACAGTAGCTCAGGAAGTGGGTTCAAGTCACTTCATCCGTACCGTTCTGGATATTTTGGTGCAGCCGTTAAGCTCCAACCTGGTTACACTGCTGGAGTAATTACTTCATTCTATCTCTCCAACAACGAAGAACATCCGGGGAACCACGATGAAATCGATATTGAGTTCCTTGGTACAACTCCTGATAAGCCCTATACTCTACAAACCAATGTGTACATTAGAGGCAGTGGAGATGGAAACATCATTGGAAGAGAGAAGAGATTTCATCTCTGGTTTGATCCAACACAGGACTTTCACAACTACGCTATATTATGGAACCCTACTGATCGATCATATTCCTGGTGGATGATGTGCCCATAA

Protein Analysis

225

Amino Acids

25.57

Weight (kDa)

6.42

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 82 - 214 3.7e-38 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44990
fragaria_vesca FvH4_3g30850
malus_domestica MD11G1146700.v1.1
prunus_persica Prupe.6G110300_v2.0.a1
pyrus_communis pycom11g11970
rosa_chinensis RchiOBHm_Chr5g0057091
rosa_laevigata RLG00000035150
rosa_multiflora Rmu_co8466263.1_g000001 Rmu_sc0001334.1_g000001 Rmu_sc0007667.1_g000003
rosa_roxburghii Rroxscaffold_1G00023110
rosa_rugosa Rorug05G0305700
rosa_samantha Rh5AG375000 Rh5BG386000 Rh5CG409500
rosa_wichuraiana Rw5G035300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 2 cut(s) 63, 283
AfaI GTAC 4 cut(s) 298, 356, 490, 530
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 61, 123, 177, 340
AjnI CCWGG 4 cut(s) 202, 291, 394, 653
AluBI AGCT 2 cut(s) 324, 388
AluI AGCT 2 cut(s) 324, 388
AlwI GGATC 1 cut(s) 587
ApeKI GCWGC 2 cut(s) 93, 377
ApoI RAATTY 2 cut(s) 63, 283
AspS9I GGNCC 2 cut(s) 191, 269
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 22, 192
AvaII GGWCC 2 cut(s) 191, 269
BaeGI GKGCMC 1 cut(s) 671
BbvI GCAGC 2 cut(s) 80, 389
BccI CCATC 2 cut(s) 23, 542
BceAI ACGGC 1 cut(s) 365
BciT130I CCWGG 4 cut(s) 204, 293, 396, 655
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 1 cut(s) 453
BfaI CTAG 2 cut(s) 216, 236
BisI GCNGC 2 cut(s) 94, 378
BlsI GCNGC 2 cut(s) 95, 379
Bme1390I CCNGG 5 cut(s) 204, 293, 396, 453, 655
Bme18I GGWCC 2 cut(s) 191, 269
BmgT120I GGNCC 2 cut(s) 191, 269
BmiI GGNNCC 7 cut(s) 47, 135, 192, 193, 270, 458, 632
BmrFI CCNGG 5 cut(s) 204, 293, 396, 453, 655
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 186, 325
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 2 cut(s) 471, 643
BsaJI CCNNGG 3 cut(s) 292, 452, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse3DI GCAATG 2 cut(s) 45, 180
BseBI CCWGG 4 cut(s) 204, 293, 396, 655
BseCI ATCGAT 2 cut(s) 471, 643
BseDI CCNNGG 3 cut(s) 292, 452, 484
BseGI GGATG 3 cut(s) 349, 448, 666
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMI GCAATG 2 cut(s) 45, 180
BseMII CTCAG 2 cut(s) 200, 339
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 671
BseXI GCAGC 2 cut(s) 80, 389
BsgI GTGCAG 1 cut(s) 396
BshVI ATCGAT 2 cut(s) 471, 643
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 671
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 4 cut(s) 244, 592, 640, 644
BspCNI CTCAG 2 cut(s) 199, 338
BspDI ATCGAT 2 cut(s) 471, 643
BspLI GGNNCC 7 cut(s) 47, 135, 192, 193, 270, 458, 632
BspPI GGATC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 45, 180
BsrGI TGTACA 1 cut(s) 528
BssECI CCNNGG 3 cut(s) 292, 452, 484
BssMI GATC 4 cut(s) 244, 592, 640, 644
BssT1I CCWWGG 1 cut(s) 484
Bst2UI CCWGG 4 cut(s) 204, 293, 396, 655
Bst4CI ACNGT 2 cut(s) 320, 359
Bst6I CTCTTC 2 cut(s) 559, 566
BstAUI TGTACA 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 186, 325
BstF5I GGATG 3 cut(s) 349, 448, 666
BstKTI GATC 4 cut(s) 247, 595, 643, 647
BstMBI GATC 4 cut(s) 244, 592, 640, 644
BstNI CCWGG 4 cut(s) 204, 293, 396, 655
BstSCI CCNGG 5 cut(s) 202, 291, 394, 451, 653
BstSLI GKGCMC 1 cut(s) 671
BstV1I GCAGC 2 cut(s) 80, 389
Bsu15I ATCGAT 2 cut(s) 471, 643
BsuTUI ATCGAT 2 cut(s) 471, 643
BtsCI GGATG 3 cut(s) 349, 448, 666
BtsI GCAGTG 2 cut(s) 402, 547
BtsIMutI CAGTG 2 cut(s) 402, 547
Cfr13I GGNCC 2 cut(s) 191, 269
ClaI ATCGAT 2 cut(s) 471, 643
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 4 cut(s) 297, 355, 489, 529
CviAII CATG 1 cut(s) 130
CviJI RGCY 8 cut(s) 48, 134, 207, 313, 324, 380, 388, 505
CviKI_1 RGCY 8 cut(s) 48, 134, 207, 313, 324, 380, 388, 505
CviQI GTAC 4 cut(s) 297, 355, 489, 529
DdeI CTNAG 2 cut(s) 186, 325
DpnI GATC 4 cut(s) 246, 594, 642, 646
DpnII GATC 4 cut(s) 244, 592, 640, 644
DraI TTTAAA 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 559, 566
EarI CTCTTC 2 cut(s) 559, 566
Eco130I CCWWGG 1 cut(s) 484
Eco47I GGWCC 2 cut(s) 191, 269
EcoO109I RGGNCCY 2 cut(s) 191, 269
EcoRII CCWGG 4 cut(s) 202, 291, 394, 653
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 1 cut(s) 133
FaiI YATR 9 cut(s) 6, 71, 131, 210, 510, 623, 628, 649, 673
FaqI GGGAC 1 cut(s) 177
FatI CATG 1 cut(s) 129
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 2 cut(s) 94, 378
FokI GGATG 2 cut(s) 336, 435
Fsp4HI GCNGC 2 cut(s) 94, 378
FspBI CTAG 2 cut(s) 216, 236
GluI GCNGC 2 cut(s) 94, 378
GsuI CTGGAG 1 cut(s) 429
HapII CCGG 1 cut(s) 452
Hin1II CATG 1 cut(s) 133
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 22, 192
Hpy166II GTNNAC 1 cut(s) 529
Hpy188III TCNNGA 3 cut(s) 327, 363, 497
Hpy8I GTNNAC 1 cut(s) 529
HpyAV CCTTC 2 cut(s) 112, 164
HpyCH4III ACNGT 2 cut(s) 320, 359
HpyCH4V TGCA 4 cut(s) 4, 38, 96, 377
HpyF3I CTNAG 2 cut(s) 186, 325
Hsp92II CATG 1 cut(s) 133
KflI GGGWCCC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 4 cut(s) 244, 592, 640, 644
LmnI GCTCC 3 cut(s) 45, 139, 393
Lsp1109I GCAGC 2 cut(s) 80, 389
MabI ACCWGGT 1 cut(s) 394
MaeI CTAG 2 cut(s) 216, 236
MaeIII GTNAC 2 cut(s) 342, 398
MalI GATC 4 cut(s) 246, 594, 642, 646
MboI GATC 4 cut(s) 244, 592, 640, 644
MboII GAAGA 6 cut(s) 118, 136, 165, 455, 576, 583
MhlI GDGCHC 1 cut(s) 671
MluCI AATT 5 cut(s) 63, 118, 225, 283, 414
MmeI TCCRAC 3 cut(s) 415, 459, 620
MnlI CCTC 5 cut(s) 91, 147, 208, 282, 530
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 117, 222, 302, 384
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 5 cut(s) 204, 293, 396, 453, 655
MvaI CCWGG 4 cut(s) 204, 293, 396, 655
NciI CCSGG 1 cut(s) 453
NdeII GATC 4 cut(s) 244, 592, 640, 644
NlaIII CATG 1 cut(s) 133
NlaIV GGNNCC 7 cut(s) 47, 135, 192, 193, 270, 458, 632
NmuCI GTSAC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PkrI GCNGC 2 cut(s) 95, 379
PpuMI RGGWCCY 2 cut(s) 191, 269
Psp5II RGGWCCY 2 cut(s) 191, 269
Psp6I CCWGG 4 cut(s) 202, 291, 394, 653
PspGI CCWGG 4 cut(s) 202, 291, 394, 653
PspN4I GGNNCC 7 cut(s) 47, 135, 192, 193, 270, 458, 632
PspPI GGNCC 2 cut(s) 191, 269
PspPPI RGGWCCY 2 cut(s) 191, 269
RsaI GTAC 4 cut(s) 298, 356, 490, 530
RsaNI GTAC 4 cut(s) 297, 355, 489, 529
SaqAI TTAA 4 cut(s) 117, 222, 302, 384
SatI GCNGC 2 cut(s) 94, 378
Sau3AI GATC 4 cut(s) 244, 592, 640, 644
Sau96I GGNCC 2 cut(s) 191, 269
ScrFI CCNGG 5 cut(s) 204, 293, 396, 453, 655
SduI GDGCHC 1 cut(s) 671
SetI ASST 6 cut(s) 35, 55, 205, 326, 390, 397
SexAI ACCWGGT 1 cut(s) 394
SinI GGWCC 2 cut(s) 191, 269
Sse9I AATT 5 cut(s) 63, 118, 225, 283, 414
SspMI CTAG 2 cut(s) 216, 236
StyD4I CCNGG 5 cut(s) 202, 291, 394, 451, 653
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 320, 359
TaqI TCGA 2 cut(s) 471, 643
TasI AATT 5 cut(s) 63, 118, 225, 283, 414
TatI WGTACW 1 cut(s) 528
Tru1I TTAA 4 cut(s) 117, 222, 302, 384
Tru9I TTAA 4 cut(s) 117, 222, 302, 384
TscAI CASTG 2 cut(s) 409, 547
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 2 cut(s) 93, 377
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 5 cut(s) 118, 338, 411, 480, 569
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 409, 547
VpaK11BI GGWCC 2 cut(s) 191, 269
XapI RAATTY 2 cut(s) 63, 283
XspI CTAG 2 cut(s) 216, 236
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.