pycom11g12620

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008659
Physical Location & Seq
Forward (+)
11520560 .. 11523492
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1896 bp
ATGGCTCCAGCTGGGAAGGTTTCTGAGTTTTGCAGACAAGGCAATGACTGGTTTTGCAAAGCGGGATTGCCAAGTGATATTACTGTGGTCGTGGACGGCGTGAGCTTCCATCTTCATAAGTTTCCTCTCATATCAAAATGTGGGAAGATATCACATATGTACGAAGAGTCCAAAAGCACACGCAAGAGCATTTTCACCGCAATTCTGGAGGAACTCCCTGGTGGCCCTGACATGTTTCTGATTGCAGCTAAATTCTGCTATGGTATGCGAGTGGAATTGACTCCAAGAAATGTAGTAATCGTGTACTGCGCAGCGGACTACCTTGAAATGACAGATGAGTATGGAGAAGGAAACTTGCTCTCAAAGTCGGAGAGTTGCTTCCATAAGAATGTACTTCACAACTGGAAAGACTCTATCTTGGCTCTTCAAAGTTGTGAGACTGTAATTGAAAGGGCAGAAAAGCTTCACATAGTAAACAAGTGTGTAAATGCTATATCGGTGATGGTTTGTACAGATCCTAGTTTGTTTGGATGGCCCATGATGATGTATGGTAGCTTACAGAGTCCTGGTGGAAGCATCCTATGGAACGGAATTAACACTGGAGCGAGAATTCGAAGCTCTGAGTCTAACTGGTGGTTTGAAGACATCTCATACCTTAGTGTGAGTTTGTTTGAGAGGCTTATCAAGACAATGGAAACAAGGGGTATAAGACCCGAAAACCTAGTAGGTGCTATAATGCATTATACCAGAAAGTACCTGCCTGGCCTAGGACGATGGCAGGGTGGACAACACGGTATACCCAGAACAGTTACAAGTTTTAGTTTGACGCCCATTGCCCTTGATCAAAGGGTAGTTTTAGAAAGCATTGAAAAACTTCTTCCCCTCAAGAAGGGGAAATCATTTTGCCGTTTTCTACTTGGTCTTCTTCGTGTTGCATTAATATTGGGTGTCAGTCAAACCTGCAAGGATGCTTTGGAGAGGAGAATAGGGATGCAGCTGGAATTGGCAACCCTAGATGGTCTTCTGATTCCTACTTATTCAGATACTGATGCATTGTATGATGCTGACTGTGTTGAACGAATAATCCATCATTTTATGTCGTCAGAATCAAAGGCTACACCATTTTCTCCGTCATCATTTGACATGGAAACATCGCCATCATCAAGACCTTTACAGAAAGTTGCAAAGTTGGTAGATAGCTATATTGCGGAGGTTGCTTCGGATGTGACTTTAAAACCAGGAAAGATACGCTCTCTTGCGGAGGCCCTCCCAGAATATTCAAGAACTTTATATGATGGGTTGTATAGGTCACTGGATATATACTTCAAGGCACACCCTTGGCTCTCCAATAAAGAGAAGGAAGATCTCTGCAGCATCATTGACTTCCAGAAGCTATCTATTGATGCATGTTCCCATGCATCTCAAAATGAGAGGTTGCCACTTAGAGTTGTTCTTCAAGTCCTGTTCTTTGAGCAGATGCATTTGAGAACCGCTCTAGCTGGTTGTCTTCATGGCTTGGAAACTCAAAGTGGCCCTGCAGGTCCTGCGACTGTCCCGGGTGACATGGCTGGCCAGATTATACAGAGGGATGGGTGGGTGACTATGGTGCGTGAGAATCGCGTTTTAAAGGTGGATATGGAAAAGATGAGATCTAGAGTTGGTGAACTCGAAGAAGAGTTCAGTAAAATAAAGCAAGAGATGAAAAAGGTGAGCAAGTCACATAGCTCCCTCAGTTCTCCTCGTGTGGTTGGCAGGAGGATTGGGTGCAGTCTTCTCTCGAGACCCTCTGATGCTAAACCAGATACTGTTGAGAGTACTCGGCCATCCCCAAGATCATCAGTTGAGCAGGCACGTCCTTCGACCTTGCATTATAGACACAAGAAAATTTCCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

632

Amino Acids

70.65

Weight (kDa)

8.55

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 210 - 470 5.1e-87 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1894
Acc16I TGCGCA 1 cut(s) 310
Acc36I ACCTGC 3 cut(s) 765, 968, 1529
AccBSI CCGCTC 1 cut(s) 1493
AccI GTMKAC 1 cut(s) 796
AccII CGCG 1 cut(s) 1620
AciI CCGC 6 cut(s) 62, 198, 314, 1208, 1259, 1491
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 2 cut(s) 1570, 1820
AcsI RAATTY 3 cut(s) 251, 609, 1884
AcyI GRCGYC 1 cut(s) 827
AfaI GTAC 6 cut(s) 161, 305, 393, 511, 755, 1816
AfiI CCNNNNNNNGG 2 cut(s) 767, 889
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 9 cut(s) 326, 428, 449, 641, 869, 1076, 1281, 1327, 1457
AjiI CACGTC 1 cut(s) 1853
AjnI CCWGG 4 cut(s) 217, 565, 760, 1237
AloI GAACNNNNNNTCC 2 cut(s) 1068, 1100
Alw26I GTCTC 2 cut(s) 431, 1774
AlwI GGATC 1 cut(s) 509
AlwNI CAGNNNCTG 3 cut(s) 1046, 1544, 1805
Ama87I CYCGRG 2 cut(s) 1555, 1777
AoxI GGCC 7 cut(s) 223, 533, 763, 1263, 1531, 1570, 1820
ApeKI GCWGC 4 cut(s) 245, 311, 994, 1371
ApoI RAATTY 3 cut(s) 251, 609, 1884
AseI ATTAAT 1 cut(s) 938
Asp700I GAANNNNTTC 2 cut(s) 462, 873
AspA2I CCTAGG 1 cut(s) 766
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 5 cut(s) 224, 534, 1264, 1532, 1541
AsuC2I CCSGG 2 cut(s) 1556, 1557
AsuHPI GGTGA 6 cut(s) 187, 511, 1571, 1609, 1673, 1720
AsuII TTCGAA 1 cut(s) 613
AvaI CYCGRG 2 cut(s) 1555, 1777
AvaII GGWCC 1 cut(s) 1541
AvrII CCTAGG 1 cut(s) 766
BalI TGGCCA 1 cut(s) 1572
BauI CACGAG 1 cut(s) 1740
BbsI GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BbvI GCAGC 4 cut(s) 257, 323, 1006, 1383
BceAI ACGGC 2 cut(s) 112, 891
BcgI CGANNNNNNTGC 2 cut(s) 1839, 1873
BciT130I CCWGG 4 cut(s) 219, 567, 762, 1239
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 1556, 1557
BcoDI GTCTC 2 cut(s) 431, 1774
BfaI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
BfmI CTRYAG 2 cut(s) 1369, 1536
BfuAI ACCTGC 3 cut(s) 765, 968, 1529
BglII AGATCT 2 cut(s) 1363, 1649
BisI GCNGC 4 cut(s) 246, 312, 995, 1372
BlnI CCTAGG 1 cut(s) 766
BlsI GCNGC 4 cut(s) 247, 313, 996, 1373
BmcAI AGTACT 1 cut(s) 1816
Bme1390I CCNGG 6 cut(s) 219, 567, 762, 1239, 1556, 1557
Bme18I GGWCC 1 cut(s) 1541
BmeT110I CYCGRG 2 cut(s) 1555, 1777
BmgBI CACGTC 1 cut(s) 1853
BmgT120I GGNCC 5 cut(s) 224, 534, 1264, 1532, 1541
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 6 cut(s) 219, 567, 762, 1239, 1556, 1557
BpiI GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BplI GAGNNNNNCTC 2 cut(s) 1477, 1509
BpmI CTGGAG 2 cut(s) 227, 621
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 2 cut(s) 1556, 1557
BsaHI GRCGYC 1 cut(s) 827
BsaI GGTCTC 1 cut(s) 1774
BsaJI CCNNGG 4 cut(s) 217, 766, 1337, 1555
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 889
Bse1I ACTGG 5 cut(s) 53, 407, 604, 635, 1317
Bse3DI GCAATG 2 cut(s) 49, 831
BseBI CCWGG 4 cut(s) 219, 567, 762, 1239
BseDI CCNNGG 4 cut(s) 217, 766, 1337, 1555
BseGI GGATG 7 cut(s) 536, 576, 973, 996, 1228, 1594, 1823
BseLI CCNNNNNNNGG 2 cut(s) 767, 889
BseMI GCAATG 2 cut(s) 49, 831
BseMII CTCAG 3 cut(s) 15, 612, 1744
BseNI ACTGG 5 cut(s) 53, 407, 604, 635, 1317
BseRI GAGGAG 2 cut(s) 994, 1728
BseXI GCAGC 4 cut(s) 257, 323, 1006, 1383
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 1786
Bsh1236I CGCG 1 cut(s) 1620
BshFI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BsiHKCI CYCGRG 2 cut(s) 1555, 1777
BsiSI CCGG 1 cut(s) 1556
BslFI GGGAC 1 cut(s) 1538
BslI CCNNNNNNNGG 2 cut(s) 767, 889
BsmAI GTCTC 2 cut(s) 431, 1774
BsmFI GGGAC 1 cut(s) 1538
BsnI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
Bso31I GGTCTC 1 cut(s) 1774
BsoBI CYCGRG 2 cut(s) 1555, 1777
Bsp119I TTCGAA 1 cut(s) 613
Bsp1407I TGTACA 1 cut(s) 509
Bsp143I GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BspACI CCGC 6 cut(s) 62, 198, 314, 1208, 1259, 1491
BspANI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BspCNI CTCAG 3 cut(s) 16, 613, 1743
BspFNI CGCG 1 cut(s) 1620
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 1373, 1540
BspMI ACCTGC 3 cut(s) 765, 968, 1529
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 429
BspT104I TTCGAA 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 1774
BsrBI CCGCTC 1 cut(s) 1493
BsrDI GCAATG 2 cut(s) 49, 831
BsrGI TGTACA 1 cut(s) 509
BsrI ACTGG 5 cut(s) 53, 407, 604, 635, 1317
BssECI CCNNGG 4 cut(s) 217, 766, 1337, 1555
BssMI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BssNAI GTATAC 1 cut(s) 797
BssNI GRCGYC 1 cut(s) 827
BssSI CACGAG 1 cut(s) 1740
BssT1I CCWWGG 2 cut(s) 766, 1337
Bst1107I GTATAC 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 1740
Bst2UI CCWGG 4 cut(s) 219, 567, 762, 1239
Bst4CI ACNGT 7 cut(s) 85, 442, 794, 808, 1070, 1552, 1807
Bst6I CTCTTC 3 cut(s) 159, 429, 1668
BstACI GRCGYC 1 cut(s) 827
BstAPI GCANNNNNTGC 1 cut(s) 1544
BstAUI TGTACA 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 1570, 1848
BstDEI CTNAG 5 cut(s) 24, 621, 656, 1442, 1730
BstENI CCTNNNNNAGG 2 cut(s) 765, 887
BstF5I GGATG 7 cut(s) 536, 576, 973, 996, 1228, 1594, 1823
BstFNI CGCG 1 cut(s) 1620
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 5 cut(s) 517, 844, 1366, 1652, 1835
BstMAI GTCTC 2 cut(s) 431, 1774
BstMBI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BstMWI GCNNNNNNNGC 3 cut(s) 39, 1214, 1544
BstNI CCWGG 4 cut(s) 219, 567, 762, 1239
BstNSI RCATGY 2 cut(s) 235, 1410
BstSCI CCNGG 6 cut(s) 217, 565, 760, 1237, 1554, 1555
BstSFI CTRYAG 2 cut(s) 1369, 1536
BstUI CGCG 1 cut(s) 1620
BstV1I GCAGC 4 cut(s) 257, 323, 1006, 1383
BstV2I GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BstX2I RGATCY 3 cut(s) 514, 1363, 1649
BstYI RGATCY 3 cut(s) 514, 1363, 1649
BstZ17I GTATAC 1 cut(s) 797
BsuRI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BtgZI GCGATG 1 cut(s) 1137
BtrI CACGTC 1 cut(s) 1853
BtsCI GGATG 7 cut(s) 536, 576, 973, 996, 1228, 1594, 1823
BtsIMutI CAGTG 2 cut(s) 597, 1310
BveI ACCTGC 3 cut(s) 765, 968, 1529
Cac8I GCNNGC 2 cut(s) 1570, 1848
CaiI CAGNNNCTG 3 cut(s) 1046, 1544, 1805
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 5 cut(s) 224, 534, 1264, 1532, 1541
Cfr9I CCCGGG 1 cut(s) 1555
CseI GACGC 1 cut(s) 835
Csp6I GTAC 6 cut(s) 160, 304, 392, 510, 754, 1815
CviAII CATG 7 cut(s) 232, 538, 1144, 1407, 1415, 1511, 1564
CviQI GTAC 6 cut(s) 160, 304, 392, 510, 754, 1815
DdeI CTNAG 5 cut(s) 24, 621, 656, 1442, 1730
DpnI GATC 5 cut(s) 516, 843, 1365, 1651, 1834
DpnII GATC 5 cut(s) 514, 841, 1363, 1649, 1832
DraI TTTAAA 2 cut(s) 1233, 1626
EaeI YGGCCR 2 cut(s) 1570, 1820
Eam1104I CTCTTC 3 cut(s) 159, 429, 1668
EarI CTCTTC 3 cut(s) 159, 429, 1668
Eco130I CCWWGG 2 cut(s) 766, 1337
Eco31I GGTCTC 1 cut(s) 1774
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 1541
Eco88I CYCGRG 2 cut(s) 1555, 1777
EcoNI CCTNNNNNAGG 2 cut(s) 765, 887
EcoO109I RGGNCCY 2 cut(s) 1264, 1541
EcoRI GAATTC 1 cut(s) 609
EcoRII CCWGG 4 cut(s) 217, 565, 760, 1237
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 766, 1337
EcoT22I ATGCAT 5 cut(s) 741, 1054, 1408, 1420, 1482
ErhI CCWWGG 2 cut(s) 766, 1337
FaeI CATG 7 cut(s) 235, 541, 1147, 1410, 1418, 1514, 1567
FaqI GGGAC 1 cut(s) 1538
FatI CATG 7 cut(s) 231, 537, 1143, 1406, 1414, 1510, 1563
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 4 cut(s) 246, 312, 995, 1372
FokI GGATG 7 cut(s) 543, 563, 980, 1003, 1235, 1601, 1810
Fsp4HI GCNGC 4 cut(s) 246, 312, 995, 1372
FspBI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
FspI TGCGCA 1 cut(s) 310
GlaI GCGC 1 cut(s) 310
GluI GCNGC 4 cut(s) 246, 312, 995, 1372
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 2 cut(s) 227, 621
HaeIII GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
HapII CCGG 1 cut(s) 1556
HgaI GACGC 1 cut(s) 835
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 827
Hin1II CATG 7 cut(s) 235, 541, 1147, 1410, 1418, 1514, 1567
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 8 cut(s) 167, 280, 410, 562, 623, 1027, 1106, 1615
HpaII CCGG 1 cut(s) 1556
HphI GGTGA 6 cut(s) 187, 511, 1571, 1609, 1673, 1720
Hpy166II GTNNAC 6 cut(s) 94, 304, 475, 785, 797, 1664
Hpy188I TCNGA 9 cut(s) 25, 240, 370, 622, 1026, 1042, 1105, 1222, 1789
Hpy188III TCNNGA 9 cut(s) 206, 685, 886, 1164, 1281, 1387, 1653, 1777, 1779
Hpy8I GTNNAC 6 cut(s) 94, 304, 475, 785, 797, 1664
HpyAV CCTTC 5 cut(s) 10, 341, 883, 1351, 1866
HpyCH4III ACNGT 7 cut(s) 85, 442, 794, 808, 1070, 1552, 1807
HpyCH4IV ACGT 1 cut(s) 1852
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 1214, 1544
HpyF3I CTNAG 5 cut(s) 24, 621, 656, 1442, 1730
HpySE526I ACGT 1 cut(s) 1852
Hsp92I GRCGYC 1 cut(s) 827
Hsp92II CATG 7 cut(s) 235, 541, 1147, 1410, 1418, 1514, 1567
HspAI GCGC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 5 cut(s) 514, 841, 1363, 1649, 1832
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 10, 602, 1730
Lsp1109I GCAGC 4 cut(s) 257, 323, 1006, 1383
MaeI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
MaeII ACGT 1 cut(s) 1852
MaeIII GTNAC 6 cut(s) 808, 1225, 1308, 1559, 1597, 1716
MalI GATC 5 cut(s) 516, 843, 1365, 1651, 1834
MbiI CCGCTC 1 cut(s) 1493
MboI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
MflI RGATCY 3 cut(s) 514, 1363, 1649
MlsI TGGCCA 1 cut(s) 1572
MluCI AATT 8 cut(s) 201, 251, 275, 444, 591, 609, 1001, 1884
MluNI TGGCCA 1 cut(s) 1572
MlyI GAGTC 5 cut(s) 176, 274, 404, 571, 632
MmeI TCCRAC 1 cut(s) 348
Mox20I TGGCCA 1 cut(s) 1572
Mph1103I ATGCAT 5 cut(s) 741, 1054, 1408, 1420, 1482
MroXI GAANNNNTTC 2 cut(s) 462, 873
MscI TGGCCA 1 cut(s) 1572
MseI TTAA 4 cut(s) 594, 938, 1232, 1625
MslI CAYNNNNRTG 2 cut(s) 387, 542
Msp20I TGGCCA 1 cut(s) 1572
MspA1I CMGCKG 3 cut(s) 11, 314, 997
MspI CCGG 1 cut(s) 1556
MspR9I CCNGG 6 cut(s) 219, 567, 762, 1239, 1556, 1557
MvaI CCWGG 4 cut(s) 219, 567, 762, 1239
MvnI CGCG 1 cut(s) 1620
MwoI GCNNNNNNNGC 3 cut(s) 39, 1214, 1544
NciI CCSGG 2 cut(s) 1556, 1557
NdeI CATATG 1 cut(s) 156
NdeII GATC 5 cut(s) 514, 841, 1363, 1649, 1832
NlaIII CATG 7 cut(s) 235, 541, 1147, 1410, 1418, 1514, 1567
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 1798
NmuCI GTSAC 5 cut(s) 1225, 1308, 1559, 1597, 1716
NsbI TGCGCA 1 cut(s) 310
NsiI ATGCAT 5 cut(s) 741, 1054, 1408, 1420, 1482
NspI RCATGY 2 cut(s) 235, 1410
NspV TTCGAA 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 1777
PciI ACATGT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdmI GAANNNNTTC 2 cut(s) 462, 873
PfeI GAWTC 3 cut(s) 1027, 1106, 1615
PkrI GCNGC 4 cut(s) 247, 313, 996, 1373
PleI GAGTC 5 cut(s) 175, 274, 404, 570, 631
PpsI GAGTC 5 cut(s) 175, 274, 404, 570, 631
PpuMI RGGWCCY 1 cut(s) 1541
PscI ACATGT 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 938
PsiI TTATAA 1 cut(s) 1894
Psp5II RGGWCCY 1 cut(s) 1541
Psp6I CCWGG 4 cut(s) 217, 565, 760, 1237
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 4 cut(s) 217, 565, 760, 1237
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 5 cut(s) 224, 534, 1264, 1532, 1541
PspPPI RGGWCCY 1 cut(s) 1541
PstI CTGCAG 2 cut(s) 1373, 1540
PstNI CAGNNNCTG 3 cut(s) 1046, 1544, 1805
PsuI RGATCY 3 cut(s) 514, 1363, 1649
PvuII CAGCTG 2 cut(s) 11, 997
RsaI GTAC 6 cut(s) 161, 305, 393, 511, 755, 1816
RsaNI GTAC 6 cut(s) 160, 304, 392, 510, 754, 1815
RseI CAYNNNNRTG 2 cut(s) 387, 542
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 594, 938, 1232, 1625
SatI GCNGC 4 cut(s) 246, 312, 995, 1372
Sau3AI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
Sau96I GGNCC 5 cut(s) 224, 534, 1264, 1532, 1541
SbfI CCTGCAGG 1 cut(s) 1540
ScaI AGTACT 1 cut(s) 1816
SchI GAGTC 5 cut(s) 176, 274, 404, 571, 632
ScrFI CCNGG 6 cut(s) 219, 567, 762, 1239, 1556, 1557
SdaI CCTGCAGG 1 cut(s) 1540
SfcI CTRYAG 2 cut(s) 1369, 1536
Sfr274I CTCGAG 1 cut(s) 1777
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 1 cut(s) 1541
SlaI CTCGAG 1 cut(s) 1777
SmaI CCCGGG 1 cut(s) 1557
SmiMI CAYNNNNRTG 2 cut(s) 387, 542
SmlI CTYRAG 2 cut(s) 884, 1777
SmoI CTYRAG 2 cut(s) 884, 1777
Sse8387I CCTGCAGG 1 cut(s) 1540
Sse9I AATT 8 cut(s) 201, 251, 275, 444, 591, 609, 1001, 1884
SsiI CCGC 6 cut(s) 62, 198, 314, 1208, 1259, 1491
SspI AATATT 2 cut(s) 942, 1277
SspMI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
StyD4I CCNGG 6 cut(s) 217, 565, 760, 1237, 1554, 1555
StyI CCWWGG 2 cut(s) 766, 1337
TaaI ACNGT 7 cut(s) 85, 442, 794, 808, 1070, 1552, 1807
TaiI ACGT 1 cut(s) 1855
TaqI TCGA 4 cut(s) 613, 1668, 1778, 1859
TasI AATT 8 cut(s) 201, 251, 275, 444, 591, 609, 1001, 1884
TatI WGTACW 4 cut(s) 303, 391, 509, 1814
TfiI GAWTC 3 cut(s) 1027, 1106, 1615
Tru1I TTAA 4 cut(s) 594, 938, 1232, 1625
Tru9I TTAA 4 cut(s) 594, 938, 1232, 1625
TscAI CASTG 2 cut(s) 604, 1317
TseFI GTSAC 5 cut(s) 1225, 1308, 1559, 1597, 1716
TseI GCWGC 4 cut(s) 245, 311, 994, 1371
Tsp45I GTSAC 5 cut(s) 1225, 1308, 1559, 1597, 1716
TspDTI ATGAA 3 cut(s) 104, 1499, 1715
TspGWI ACGGA 2 cut(s) 603, 1119
TspMI CCCGGG 1 cut(s) 1555
TspRI CASTG 2 cut(s) 604, 1317
VpaK11BI GGWCC 1 cut(s) 1541
VspI ATTAAT 1 cut(s) 938
XagI CCTNNNNNAGG 2 cut(s) 765, 887
XapI RAATTY 3 cut(s) 251, 609, 1884
XbaI TCTAGA 1 cut(s) 1652
XceI RCATGY 2 cut(s) 235, 1410
XhoI CTCGAG 1 cut(s) 1777
XmaI CCCGGG 1 cut(s) 1555
XmaJI CCTAGG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 2 cut(s) 462, 873
XspI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
ZrmI AGTACT 1 cut(s) 1816
Zsp2I ATGCAT 5 cut(s) 741, 1054, 1408, 1420, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.