pycom11g16860

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012256
Physical Location & Seq
Forward (+)
18689433 .. 18690915
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGATGGTGGGGGCTGCTCTGTCTGTGGTGGGTTCGTCGGTGGTGGACTCGCACACTAGTCCTTGCCTGTGTTTGGATGCGCTGCCGACTACGGCGATGAATCTCAAGAGCGGGGGAGAAATGGTTTTGCAGAGGAATTCAATGGCCAAGAAGCATGTGGTGAAACCAGGCTCCTTGGAGTTGGGCAGTTCGTTCATTGATTCCGGCCACGACAGGCGATTTTCGATGAAAGCTGTTCGTGGTATTGGCAGTAGGAGTACGAGGAAGGGAAAGAACCGGGGTTTTGTAATTGTTAATGAGCTTGCAGGGCAATATGAAGACAGTTTTGAGGATGTTAAAACGCAATTACTCAACTATTTTACGTACAAGGCTGTGAGGACTGTTATGAACCAGCTGTATGAGATGAACCCAACACAATATAGGTGGTTGTACGATTTTGTTGTAACTCACAAGCCTGGAGACGGGAAGCGGTTCCTCCGCACCCTGGGGAAGGAGAGGCATGAGCTTGCTGAGAGAGTAATGGTGACACGGCTTCACCTCTATGGTAAATGGGTCAAGAAATGCGATCATGCCGCAATATATCAAGGAATCGCAGACGAGAACTTGGAGTTGATGCGTGAACGGCTTACTGAGACTGTGATATGGCCCTCCGATGACAACACGGAGAAGATTGGCTGA

Protein Analysis

226

Amino Acids

25.45

Weight (kDa)

9.18

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 112 - 212 7e-20 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19855 AT5G19855
fragaria_vesca FvH4_3g28850
malus_domestica MD03G1175000.v1.1 MD11G1193100.v1.1
prunus_persica Prupe.4G249500_v2.0.a1 Prupe.4G249500_v2.0.a1
pyrus_communis pycom03g13250 pycom11g16860
rosa_chinensis RchiOBHm_Chr5g0052051
rosa_laevigata RLG00000034846
rosa_multiflora Rmu_ssc0000098.1_g000001
rosa_roxburghii Rroxscaffold_1G00027850
rosa_rugosa Rorug05G0275200
rosa_samantha Rh5AG347200 Rh5BG358100 Rh5CG383600 Rh5DG373600
rosa_wichuraiana Rw5G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 111
AciI CCGC 4 cut(s) 111, 469, 478, 573
AcoI YGGCCR 2 cut(s) 144, 205
AcsI RAATTY 1 cut(s) 136
AfaI GTAC 3 cut(s) 259, 365, 431
AfiI CCNNNNNNNGG 4 cut(s) 73, 461, 484, 490
AgsI TTSAA 1 cut(s) 141
AhlI ACTAGT 1 cut(s) 56
AjnI CCWGG 3 cut(s) 166, 454, 483
AluBI AGCT 4 cut(s) 233, 301, 394, 505
AluI AGCT 4 cut(s) 233, 301, 394, 505
Alw26I GTCTC 2 cut(s) 453, 626
AoxI GGCC 3 cut(s) 144, 205, 644
ApeKI GCWGC 2 cut(s) 14, 82
ApoI RAATTY 1 cut(s) 136
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 645
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 172, 527, 535
BalI TGGCCA 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 3 cut(s) 108, 545, 638
BciT130I CCWGG 3 cut(s) 168, 456, 485
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 453, 626
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 1 cut(s) 57
BisI GCNGC 3 cut(s) 15, 83, 573
BlsI GCNGC 3 cut(s) 16, 84, 574
Bme1390I CCNGG 4 cut(s) 168, 278, 456, 485
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 2 cut(s) 172, 473
BmrFI CCNGG 4 cut(s) 168, 278, 456, 485
BmsI GCATC 2 cut(s) 67, 603
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 363
BsaJI CCNNGG 4 cut(s) 174, 277, 483, 484
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 461, 484, 490
BseBI CCWGG 3 cut(s) 168, 456, 485
BseDI CCNNGG 4 cut(s) 174, 277, 483, 484
BseGI GGATG 2 cut(s) 82, 337
BseLI CCNNNNNNNGG 4 cut(s) 73, 461, 484, 490
BseMII CTCAG 2 cut(s) 501, 621
BseXI GCAGC 1 cut(s) 69
BshFI GGCC 3 cut(s) 146, 207, 646
BsiSI CCGG 2 cut(s) 204, 277
BslI CCNNNNNNNGG 4 cut(s) 73, 461, 484, 490
BsmAI GTCTC 2 cut(s) 453, 626
BsmBI CGTCTC 1 cut(s) 453
BsnI GGCC 3 cut(s) 146, 207, 646
Bsp143I GATC 1 cut(s) 565
BspACI CCGC 4 cut(s) 111, 469, 478, 573
BspANI GGCC 3 cut(s) 146, 207, 646
BspCNI CTCAG 2 cut(s) 502, 622
BspLI GGNNCC 2 cut(s) 172, 473
BsrBI CCGCTC 1 cut(s) 111
BssECI CCNNGG 4 cut(s) 174, 277, 483, 484
BssMI GATC 1 cut(s) 565
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 3 cut(s) 168, 456, 485
Bst4CI ACNGT 3 cut(s) 323, 382, 637
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 303, 507
BstDEI CTNAG 2 cut(s) 510, 630
BstENI CCTNNNNNAGG 1 cut(s) 488
BstF5I GGATG 2 cut(s) 82, 337
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 1 cut(s) 568
BstMAI GTCTC 2 cut(s) 453, 626
BstMBI GATC 1 cut(s) 565
BstMWI GCNNNNNNNGC 2 cut(s) 307, 622
BstNI CCWGG 3 cut(s) 168, 456, 485
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 166, 276, 454, 483
BstSNI TACGTA 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 146, 207, 646
BtgZI GCGATG 1 cut(s) 110
BtsCI GGATG 2 cut(s) 82, 337
Cac8I GCNNGC 2 cut(s) 303, 507
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 645
Csp6I GTAC 3 cut(s) 258, 364, 430
CspCI CAANNNNNGTGG 2 cut(s) 404, 439
CviAII CATG 3 cut(s) 155, 500, 569
CviQI GTAC 3 cut(s) 258, 364, 430
DdeI CTNAG 2 cut(s) 510, 630
DpnI GATC 1 cut(s) 567
DpnII GATC 1 cut(s) 565
EaeI YGGCCR 2 cut(s) 144, 205
Eco105I TACGTA 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 3 cut(s) 166, 454, 483
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 3 cut(s) 158, 503, 572
FatI CATG 3 cut(s) 154, 499, 568
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 3 cut(s) 15, 83, 573
FokI GGATG 2 cut(s) 89, 344
Fsp4HI GCNGC 3 cut(s) 15, 83, 573
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 81
GluI GCNGC 3 cut(s) 15, 83, 573
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 3 cut(s) 146, 207, 646
HapII CCGG 2 cut(s) 204, 277
HhaI GCGC 1 cut(s) 82
Hin1II CATG 3 cut(s) 158, 503, 572
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 4 cut(s) 47, 100, 200, 588
HpaII CCGG 2 cut(s) 204, 277
HphI GGTGA 3 cut(s) 172, 527, 535
Hpy166II GTNNAC 2 cut(s) 46, 620
Hpy188I TCNGA 1 cut(s) 652
Hpy188III TCNNGA 2 cut(s) 106, 556
Hpy8I GTNNAC 2 cut(s) 46, 620
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 259, 484
HpyCH4III ACNGT 3 cut(s) 323, 382, 637
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 2 cut(s) 130, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 622
HpyF3I CTNAG 2 cut(s) 510, 630
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 3 cut(s) 158, 503, 572
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 565
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 2 cut(s) 67, 603
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 442, 523
MalI GATC 1 cut(s) 567
MbiI CCGCTC 1 cut(s) 111
MboI GATC 1 cut(s) 565
MboII GAAGA 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 3 cut(s) 136, 288, 344
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 8 cut(s) 126, 255, 322, 369, 485, 489, 548, 658
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 470
MscI TGGCCA 1 cut(s) 146
MseI TTAA 2 cut(s) 294, 336
MslI CAYNNNNRTG 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 2 cut(s) 204, 277
MspR9I CCNGG 4 cut(s) 168, 278, 456, 485
MvaI CCWGG 3 cut(s) 168, 456, 485
MwoI GCNNNNNNNGC 2 cut(s) 307, 622
NciI CCSGG 1 cut(s) 278
NdeII GATC 1 cut(s) 565
NlaIII CATG 3 cut(s) 158, 503, 572
NlaIV GGNNCC 2 cut(s) 172, 473
NmuCI GTSAC 1 cut(s) 523
NspI RCATGY 1 cut(s) 158
PasI CCCWGGG 1 cut(s) 484
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 100, 200, 588
PkrI GCNGC 3 cut(s) 16, 84, 574
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Ppu21I YACGTR 1 cut(s) 363
Psp6I CCWGG 3 cut(s) 166, 454, 483
PspGI CCWGG 3 cut(s) 166, 454, 483
PspN4I GGNNCC 2 cut(s) 172, 473
PspPI GGNCC 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 3 cut(s) 259, 365, 431
RsaNI GTAC 3 cut(s) 258, 364, 430
RseI CAYNNNNRTG 1 cut(s) 540
SaqAI TTAA 2 cut(s) 294, 336
SatI GCNGC 3 cut(s) 15, 83, 573
Sau3AI GATC 1 cut(s) 565
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 4 cut(s) 168, 278, 456, 485
SetI ASST 7 cut(s) 235, 303, 365, 396, 425, 507, 540
SfaNI GCATC 2 cut(s) 67, 603
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SnaBI TACGTA 1 cut(s) 363
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 3 cut(s) 136, 288, 344
SsiI CCGC 4 cut(s) 111, 469, 478, 573
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 4 cut(s) 166, 276, 454, 483
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 3 cut(s) 323, 382, 637
TaiI ACGT 1 cut(s) 365
TaqI TCGA 1 cut(s) 224
TasI AATT 3 cut(s) 136, 288, 344
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 3 cut(s) 100, 200, 588
Tru1I TTAA 2 cut(s) 294, 336
Tru9I TTAA 2 cut(s) 294, 336
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 14, 82
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 6 cut(s) 113, 184, 242, 330, 401, 419
TspGWI ACGGA 1 cut(s) 677
XagI CCTNNNNNAGG 1 cut(s) 488
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.