pycom11g20440

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010677
Physical Location & Seq
Reverse (-)
23419703 .. 23421248
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGTATGGACATGTCGGGAGACTAGCAGAAGAAATAAAGAAAGGGGCTTCATCTGTCGAAGGTGTAGAAGCCACCCTATGGCAAGTCCCTGAGACACTCCCAGAGGAAGTTCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCTCGCCAGATGAACTGGCTGACGCAGATGGATTCGTTTTTGGCTTCCCAACAAGATTTGGTATGATGGCTGCCCAATTTAAGGCCTTTCTGGACGCAACAGGTGGTCTATGGAGAACACAACAGCTTGCTGGCAAACCTGCTGGAATCTTTTACAGCACCGCATCTCAAGGTGGCGGGCAAGAGACTACTGCATTGACAGCCATCACTCAGCTGGTTCATCATGGGATGATATTTGTACCCATCGGTTACACATTCGGAGTTGGCATGTTTGAAATGGAGAATATCAAAGGCGGAAGTCCTTACGGTGCAGGAACTTATGCTGGGGACGGTTCAAGACAGCCATCTCAGCTTGAGCTGGAGCAAGCATTCCACCAAGGGAAGTACATTGCCACCATCACAAAGAAGCTCAAGGGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

193

Amino Acids

20.34

Weight (kDa)

5.56

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 7 - 134 7.5e-14 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccB7I CCANNNNNTGG 1 cut(s) 78
AciI CCGC 3 cut(s) 315, 330, 447
AfaI GTAC 2 cut(s) 393, 539
AfiI CCNNNNNNNGG 2 cut(s) 78, 235
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 428, 489
AluBI AGCT 6 cut(s) 280, 367, 505, 511, 562, 572
AluI AGCT 6 cut(s) 280, 367, 505, 511, 562, 572
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 3 cut(s) 13, 86, 332
Alw44I GTGCAC 1 cut(s) 126
AoxI GGCC 1 cut(s) 237
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 224, 572
BaeGI GKGCMC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 211, 559
BccI CCATC 6 cut(s) 176, 214, 365, 404, 505, 557
BcoDI GTCTC 3 cut(s) 13, 86, 332
BfaI CTAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 2 cut(s) 225, 573
BlsI GCNGC 2 cut(s) 226, 574
BmsI GCATC 2 cut(s) 133, 326
BpmI CTGGAG 1 cut(s) 533
BpuEI CTTGAG 3 cut(s) 306, 527, 548
BsaJI CCNNGG 1 cut(s) 529
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 235
Bse1I ACTGG 1 cut(s) 174
Bse3DI GCAATG 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 529
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 78, 235
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 81, 377, 515
BseNI ACTGG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 2 cut(s) 211, 559
BseYI CCCAGC 1 cut(s) 476
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 130
BslFI GGGAC 2 cut(s) 71, 494
BslI CCNNNNNNNGG 2 cut(s) 78, 235
BsmAI GTCTC 3 cut(s) 13, 86, 332
BsmFI GGGAC 2 cut(s) 71, 494
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 130
BspACI CCGC 3 cut(s) 315, 330, 447
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 3 cut(s) 82, 376, 514
BspMI ACCTGC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 529
Bst4CI ACNGT 2 cut(s) 461, 485
BstC8I GCNNGC 4 cut(s) 282, 286, 332, 519
BstDEI CTNAG 3 cut(s) 90, 363, 501
BstF5I GGATG 1 cut(s) 387
BstMAI GTCTC 3 cut(s) 13, 86, 332
BstMWI GCNNNNNNNGC 2 cut(s) 353, 502
BstNSI RCATGY 2 cut(s) 14, 424
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 211, 559
BsuRI GGCC 1 cut(s) 239
BtsCI GGATG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 4 cut(s) 282, 286, 332, 519
CseI GACGC 2 cut(s) 185, 257
Csp6I GTAC 2 cut(s) 392, 538
CviAII CATG 3 cut(s) 11, 377, 421
CviQI GTAC 2 cut(s) 392, 538
DdeI CTNAG 3 cut(s) 90, 363, 501
EciI GGCGGA 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 529
Eco147I AGGCCT 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 3 cut(s) 14, 380, 424
FaiI YATR 8 cut(s) 6, 12, 79, 218, 265, 378, 422, 474
FaqI GGGAC 2 cut(s) 71, 494
FatI CATG 3 cut(s) 10, 376, 420
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 2 cut(s) 225, 573
FokI GGATG 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 225, 573
FspBI CTAG 1 cut(s) 23
GluI GCNGC 2 cut(s) 225, 573
GsaI CCCAGC 1 cut(s) 480
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 1 cut(s) 239
HgaI GACGC 2 cut(s) 185, 257
Hin1II CATG 3 cut(s) 14, 380, 424
HinfI GANTC 2 cut(s) 186, 300
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 413
Hpy188III TCNNGA 3 cut(s) 16, 245, 489
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 2 cut(s) 461, 485
HpyCH4V TGCA 4 cut(s) 128, 146, 347, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 502
HpyF3I CTNAG 3 cut(s) 90, 363, 501
Hsp92II CATG 3 cut(s) 14, 380, 424
LmnI GCTCC 2 cut(s) 514, 569
Lsp1109I GCAGC 2 cut(s) 211, 559
LweI GCATC 2 cut(s) 133, 326
MaeI CTAG 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 401
MboII GAAGA 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 1 cut(s) 230
MnlI CCTC 1 cut(s) 97
MseI TTAA 2 cut(s) 234, 577
MspA1I CMGCKG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 521
MwoI GCNNNNNNNGC 2 cut(s) 353, 502
NlaIII CATG 3 cut(s) 14, 380, 424
NspI RCATGY 2 cut(s) 14, 424
PceI AGGCCT 1 cut(s) 239
PciI ACATGT 1 cut(s) 10
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 2 cut(s) 186, 300
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 2 cut(s) 226, 574
PscI ACATGT 1 cut(s) 10
PspFI CCCAGC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 2 cut(s) 393, 539
RsaNI GTAC 2 cut(s) 392, 538
SaqAI TTAA 2 cut(s) 234, 577
SatI GCNGC 2 cut(s) 225, 573
SduI GDGCHC 1 cut(s) 130
SfaNI GCATC 2 cut(s) 133, 326
SmlI CTYRAG 3 cut(s) 321, 506, 563
SmoI CTYRAG 3 cut(s) 321, 506, 563
Sse9I AATT 1 cut(s) 230
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 3 cut(s) 315, 330, 447
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 239
StyI CCWWGG 1 cut(s) 529
TaaI ACNGT 2 cut(s) 461, 485
TaqI TCGA 1 cut(s) 57
TasI AATT 1 cut(s) 230
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 2 cut(s) 186, 300
Tru1I TTAA 2 cut(s) 234, 577
Tru9I TTAA 2 cut(s) 234, 577
TseI GCWGC 2 cut(s) 224, 572
TspDTI ATGAA 3 cut(s) 39, 180, 362
Van91I CCANNNNNTGG 1 cut(s) 78
VneI GTGCAC 1 cut(s) 126
XceI RCATGY 2 cut(s) 14, 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.