pycom11g21250

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0017061
Physical Location & Seq
Forward (+)
24393657 .. 24398633
4977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1608 bp
ATGGGCCTTTTTGTTGACTTTTTGTTGAATATATCACAAAAGTTTAAACCAACAAAAATGACGTTCAACTGTTTGTTATACCCATTCTCTGTTCTCCTCATTTCCTATTCTGTTTTAGCTCAAAGTTTGAAACCACATGAAGAAATCCAAAGGTCTGCATTCCCAAATGACTTCTTCTTTGGAGCTGCGACTTCATCTTATCAGGTTGAAGGTGCTTATCTTCAAGATGGTAAGGGCCTAAGCAACTGGGATGTTTTCACAAAAATTCCAGGAAAAATAGAGGATAATGCTAACGGAGACGTTGCAGACGACCATTACAATCTCTACTTGGAAGACATTGAGCTGATGCATTCCTTAGGCATAAGGGGATACCGGTTCTCCATTTCTTGGTCCAGAATTCTACCTGATGGGATGCTCGGCACGCCCAATCCAAGCGGGATCACGTTTTATAACAAGCTTATAAACCACCTAGTAAGCAAAGGAATAGAACCATTTGTGACAATTCACCACACTGACCTGCCCTATATGCTAGAAGCAAGAGATGGGGGCTGGCTTAGTCCGCTATTACGGGAAGAATTTGCTCATTTTGCCACCATATGCTTCAAGAATTTTGGGGATAGGGTGAGGTACTGGGTTACCATAAACGAGCCCAATCTCATAGCTGATTTAGCCTACATAAGAGGAATATTTCCACCAGCTCGTTGTTCACCGCCGTTTGGTAATTGTTCCACTGGAAATTCTGATGTGGAGCCTCTCATTGCAATGCACAACATGTTAATAGCACATGGCATGGCCGTTGACATCTATCGTAGTTTGTTTTGGCCAAAACAACAGGGGTTTATTGGAATTGTTTCGAGTGCTTTCATGTATGAACCGCTTAGAGATGAAGAAAGAGATCGGCTTGCTGCTGATAGAGCCTTGGCTTTCAATGTGGCCTGGATGCTTGATCCATTAATATATGGAGATTATCCTGCTGAAATGCGCCAATGTCATGGCTTGGCATTGCCAAAGTTTTCGGCCAATGAGAAAAGACTTTTAAGAGGAAGCATAGATTTCATTGGTGTCAACCACTATTCAACTCTATATGTCAAGGACTGTATCAACTCTACTTGCCCCTCCGGCGGTGACCGTCCCATTAGAGGCTTTCTCAACACAACTTCGTATCGAGATGGCGTCCCCATTGGAGAACCGACTGGTACGTCGTATTTATTTGTTGTTCCAAGAGGGATGGAGGAGATCATTGACTATCTTAAACGAAGATATCATAACGTGCCCATGTTTGTTACTGAAAATGGATACACTTTACCAGCACAACAAAATGAGTTACAAGACATGGGTCGGATCAATTTTCACAAAGCTTACCTTGTCTCTTTAGCCAGAGCAATAAGGAAGGGGGCTAATGTACGTGGATATTTCATTTGGTCCTTGATGGATAATTTTGAATGGCTAAGTGGATACACCTCAAGATTTGGTTTATATTATGTCGATCGTCAAACACTTGAACGAACTCCAAAGCTTTCTGCAAAATGGTTTGCTAGTTTTCTCAAAAACAATACTCATAACGAGAAGGAAGAAAGTAGTGCAGCTGCTTCATCGGTTCAGGGTTGA

Protein Analysis

536

Amino Acids

60.77

Weight (kDa)

6.41

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 51 - 518 1e-147 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 450, 461
AasI GACNNNNNNGTC 1 cut(s) 1198
Acc36I ACCTGC 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 387
AciI CCGC 5 cut(s) 435, 560, 710, 875, 1122
AclWI GGATC 3 cut(s) 446, 941, 1349
AcoI YGGCCR 3 cut(s) 792, 821, 1017
AcsI RAATTY 5 cut(s) 264, 396, 575, 607, 736
AcyI GRCGYC 1 cut(s) 1173
AfaI GTAC 3 cut(s) 629, 1198, 1404
AfiI CCNNNNNNNGG 4 cut(s) 387, 716, 1121, 1139
AflIII ACRYGT 1 cut(s) 771
AgeI ACCGGT 1 cut(s) 372
AjnI CCWGG 2 cut(s) 268, 935
AluBI AGCT 9 cut(s) 119, 185, 343, 457, 662, 698, 1358, 1516, 1586
AluI AGCT 9 cut(s) 119, 185, 343, 457, 662, 698, 1358, 1516, 1586
Alw26I GTCTC 2 cut(s) 291, 1372
AlwI GGATC 3 cut(s) 446, 941, 1349
AoxI GGCC 6 cut(s) 4, 235, 792, 821, 933, 1017
ApeKI GCWGC 4 cut(s) 185, 905, 1583, 1586
ApoI RAATTY 5 cut(s) 264, 396, 575, 607, 736
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AseI ATTAAT 1 cut(s) 953
AsiGI ACCGGT 1 cut(s) 372
Asp700I GAANNNNTTC 1 cut(s) 850
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 4 cut(s) 4, 235, 390, 1422
AsuHPI GGTGA 4 cut(s) 497, 634, 699, 1136
AvaII GGWCC 2 cut(s) 390, 1422
AxyI CCTNAGG 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 1275
BalI TGGCCA 1 cut(s) 823
BanII GRGCYC 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 172, 892, 1573, 1595
BccI CCATC 6 cut(s) 221, 401, 536, 1163, 1222, 1423
BceAI ACGGC 2 cut(s) 697, 779
BciT130I CCWGG 2 cut(s) 270, 937
BciVI GTATCC 3 cut(s) 362, 1289, 1448
BcoDI GTCTC 2 cut(s) 291, 1372
BfaI CTAG 3 cut(s) 470, 530, 1536
BfuAI ACCTGC 1 cut(s) 525
BfuI GTATCC 3 cut(s) 362, 1289, 1448
BglI GCCNNNNNGGC 1 cut(s) 1119
BisI GCNGC 4 cut(s) 186, 906, 1584, 1587
BlsI GCNGC 4 cut(s) 187, 907, 1585, 1588
Bme1390I CCNGG 2 cut(s) 270, 937
Bme18I GGWCC 2 cut(s) 390, 1422
BmgT120I GGNCC 4 cut(s) 4, 235, 390, 1422
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 2 cut(s) 270, 937
BmrI ACTGGG 2 cut(s) 256, 640
BmsI GCATC 3 cut(s) 336, 402, 930
BmuI ACTGGG 2 cut(s) 256, 640
BoxI GACNNNNGTC 1 cut(s) 1335
BpiI GAAGAC 1 cut(s) 339
BplI GAGNNNNNCTC 2 cut(s) 1131, 1163
Bpu10I CCTNAGC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 1447
BsaAI YACGTR 1 cut(s) 1406
BsaHI GRCGYC 1 cut(s) 1173
BsaJI CCNNGG 1 cut(s) 918
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 4 cut(s) 387, 716, 1121, 1139
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 4 cut(s) 251, 635, 736, 1198
Bse21I CCTNAGG 1 cut(s) 355
Bse3DI GCAATG 3 cut(s) 756, 768, 1001
BseBI CCWGG 2 cut(s) 270, 937
BseDI CCNNGG 1 cut(s) 918
BseGI GGATG 4 cut(s) 256, 417, 945, 1233
BseLI CCNNNNNNNGG 4 cut(s) 387, 716, 1121, 1139
BseMI GCAATG 3 cut(s) 756, 768, 1001
BseNI ACTGG 4 cut(s) 251, 635, 736, 1198
BseRI GAGGAG 2 cut(s) 86, 1247
BseSI GKGCMC 1 cut(s) 1275
BseXI GCAGC 4 cut(s) 172, 892, 1573, 1595
BsgI GTGCAG 1 cut(s) 1602
Bsh1285I CGRYCG 1 cut(s) 1489
BshFI GGCC 6 cut(s) 6, 237, 794, 823, 935, 1019
BshTI ACCGGT 1 cut(s) 372
BsiEI CGRYCG 1 cut(s) 1489
BsiSI CCGG 2 cut(s) 373, 1119
BslFI GGGAC 2 cut(s) 1116, 1160
BslI CCNNNNNNNGG 4 cut(s) 387, 716, 1121, 1139
BsmAI GTCTC 2 cut(s) 291, 1372
BsmBI CGTCTC 1 cut(s) 291
BsmFI GGGAC 2 cut(s) 1116, 1160
BsmI GAATGC 2 cut(s) 158, 349
BsnI GGCC 6 cut(s) 6, 237, 794, 823, 935, 1019
Bsp1286I GDGCHC 2 cut(s) 651, 1275
Bsp143I GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
BspACI CCGC 5 cut(s) 435, 560, 710, 875, 1122
BspANI GGCC 6 cut(s) 6, 237, 794, 823, 935, 1019
BspLI GGNNCC 1 cut(s) 750
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 3 cut(s) 446, 941, 1349
BsrDI GCAATG 3 cut(s) 756, 768, 1001
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 4 cut(s) 251, 635, 736, 1198
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 918
BssMI GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
BssNI GRCGYC 1 cut(s) 1173
BssT1I CCWWGG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 270, 937
Bst4CI ACNGT 3 cut(s) 71, 1097, 1130
BstACI GRCGYC 1 cut(s) 1173
BstBAI YACGTR 1 cut(s) 1406
BstC8I GCNNGC 3 cut(s) 422, 551, 903
BstDEI CTNAG 5 cut(s) 239, 355, 554, 878, 1448
BstEII GGTNACC 2 cut(s) 634, 1124
BstF5I GGATG 4 cut(s) 256, 417, 945, 1233
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 6 cut(s) 441, 898, 949, 1239, 1344, 1489
BstMAI GTCTC 2 cut(s) 291, 1372
BstMBI GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
BstMCI CGRYCG 1 cut(s) 1489
BstMWI GCNNNNNNNGC 7 cut(s) 421, 526, 559, 587, 668, 914, 1119
BstNI CCWGG 2 cut(s) 270, 937
BstNSI RCATGY 1 cut(s) 775
BstPAI GACNNNNGTC 1 cut(s) 1335
BstPI GGTNACC 2 cut(s) 634, 1124
BstSCI CCNGG 2 cut(s) 268, 935
BstSLI GKGCMC 1 cut(s) 1275
BstV1I GCAGC 4 cut(s) 172, 892, 1573, 1595
BstV2I GAAGAC 1 cut(s) 339
BstXI CCANNNNNNTGG 1 cut(s) 992
Bsu36I CCTNAGG 1 cut(s) 355
BsuI GTATCC 3 cut(s) 362, 1289, 1448
BsuRI GGCC 6 cut(s) 6, 237, 794, 823, 935, 1019
BtsCI GGATG 4 cut(s) 256, 417, 945, 1233
BtsIMutI CAGTG 2 cut(s) 510, 729
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 3 cut(s) 422, 551, 903
CfoI GCGC 1 cut(s) 984
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 4 cut(s) 4, 235, 390, 1422
CseI GACGC 1 cut(s) 1162
Csp6I GTAC 3 cut(s) 628, 1197, 1403
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 8 cut(s) 137, 772, 785, 790, 865, 992, 1276, 1333
CviQI GTAC 3 cut(s) 628, 1197, 1403
DdeI CTNAG 5 cut(s) 239, 355, 554, 878, 1448
DpnI GATC 6 cut(s) 440, 897, 948, 1238, 1343, 1488
DpnII GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
DraI TTTAAA 1 cut(s) 46
DrdI GACNNNNNNGTC 1 cut(s) 1198
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 3 cut(s) 792, 821, 1017
Eco130I CCWWGG 1 cut(s) 918
Eco24I GRGCYC 1 cut(s) 651
Eco32I GATATC 1 cut(s) 1262
Eco47I GGWCC 2 cut(s) 390, 1422
Eco81I CCTNAGG 1 cut(s) 355
Eco91I GGTNACC 2 cut(s) 634, 1124
EcoO109I RGGNCCY 1 cut(s) 235
EcoO65I GGTNACC 2 cut(s) 634, 1124
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 268, 935
EcoRV GATATC 1 cut(s) 1262
EcoT14I CCWWGG 1 cut(s) 918
EcoT22I ATGCAT 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 918
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 8 cut(s) 140, 775, 788, 793, 868, 995, 1279, 1336
FalI AAGNNNNNCTT 2 cut(s) 1347, 1379
FaqI GGGAC 2 cut(s) 1116, 1160
FatI CATG 8 cut(s) 136, 771, 784, 789, 864, 991, 1275, 1332
FauI CCCGC 1 cut(s) 428
FauNDI CATATG 1 cut(s) 596
Fnu4HI GCNGC 4 cut(s) 186, 906, 1584, 1587
FokI GGATG 4 cut(s) 263, 424, 952, 1240
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 186, 906, 1584, 1587
FspBI CTAG 3 cut(s) 470, 530, 1536
GlaI GCGC 1 cut(s) 983
GluI GCNGC 4 cut(s) 186, 906, 1584, 1587
HaeIII GGCC 6 cut(s) 6, 237, 794, 823, 935, 1019
HapII CCGG 2 cut(s) 373, 1119
HgaI GACGC 1 cut(s) 1162
HhaI GCGC 1 cut(s) 984
Hin1I GRCGYC 1 cut(s) 1173
Hin1II CATG 8 cut(s) 140, 775, 788, 793, 868, 995, 1279, 1336
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HincII GTYRAC 3 cut(s) 16, 799, 1066
HindII GTYRAC 3 cut(s) 16, 799, 1066
HindIII AAGCTT 3 cut(s) 455, 1356, 1514
HpaII CCGG 2 cut(s) 373, 1119
HphI GGTGA 4 cut(s) 497, 634, 699, 1136
Hpy166II GTNNAC 4 cut(s) 16, 707, 799, 1066
Hpy188I TCNGA 2 cut(s) 742, 1341
Hpy188III TCNNGA 5 cut(s) 224, 393, 604, 1166, 1464
Hpy8I GTNNAC 4 cut(s) 16, 707, 799, 1066
Hpy99I CGWCG 1 cut(s) 1204
HpyAV CCTTC 3 cut(s) 203, 1384, 1561
HpyCH4III ACNGT 3 cut(s) 71, 1097, 1130
HpyCH4IV ACGT 6 cut(s) 62, 300, 443, 1199, 1269, 1405
HpyCH4V TGCA 7 cut(s) 158, 305, 349, 761, 766, 1523, 1583
HpyF10VI GCNNNNNNNGC 7 cut(s) 421, 526, 559, 587, 668, 914, 1119
HpyF3I CTNAG 5 cut(s) 239, 355, 554, 878, 1448
HpySE526I ACGT 6 cut(s) 62, 300, 443, 1199, 1269, 1405
Hsp92I GRCGYC 1 cut(s) 1173
Hsp92II CATG 8 cut(s) 140, 775, 788, 793, 868, 995, 1279, 1336
HspAI GCGC 1 cut(s) 982
Kzo9I GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
LmnI GCTCC 2 cut(s) 182, 748
Lsp1109I GCAGC 4 cut(s) 172, 892, 1573, 1595
LweI GCATC 3 cut(s) 336, 402, 930
MaeI CTAG 3 cut(s) 470, 530, 1536
MaeII ACGT 6 cut(s) 62, 300, 443, 1199, 1269, 1405
MaeIII GTNAC 5 cut(s) 496, 634, 1124, 1282, 1323
MalI GATC 6 cut(s) 440, 897, 948, 1238, 1343, 1488
MboI GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
MboII GAAGA 8 cut(s) 152, 166, 212, 344, 584, 899, 1269, 1583
MhlI GDGCHC 2 cut(s) 651, 1275
MlsI TGGCCA 1 cut(s) 823
MluNI TGGCCA 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 1319
Mox20I TGGCCA 1 cut(s) 823
Mph1103I ATGCAT 1 cut(s) 351
MroXI GAANNNNTTC 1 cut(s) 850
MscI TGGCCA 1 cut(s) 823
MseI TTAA 5 cut(s) 45, 776, 953, 1037, 1251
MslI CAYNNNNRTG 1 cut(s) 761
Msp20I TGGCCA 1 cut(s) 823
MspA1I CMGCKG 1 cut(s) 1586
MspI CCGG 2 cut(s) 373, 1119
MspR9I CCNGG 2 cut(s) 270, 937
MssI GTTTAAAC 1 cut(s) 46
Mva1269I GAATGC 2 cut(s) 158, 349
MvaI CCWGG 2 cut(s) 270, 937
MwoI GCNNNNNNNGC 7 cut(s) 421, 526, 559, 587, 668, 914, 1119
NdeI CATATG 1 cut(s) 596
NdeII GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
NlaIII CATG 8 cut(s) 140, 775, 788, 793, 868, 995, 1279, 1336
NlaIV GGNNCC 1 cut(s) 750
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 496, 1124
NsiI ATGCAT 1 cut(s) 351
NspI RCATGY 1 cut(s) 775
PciI ACATGT 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 2 cut(s) 158, 349
PdmI GAANNNNTTC 1 cut(s) 850
PflMI CCANNNNNTGG 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 268
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 4 cut(s) 187, 907, 1585, 1588
Ple19I CGATCG 1 cut(s) 1489
PmeI GTTTAAAC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 1406
PscI ACATGT 1 cut(s) 771
PshAI GACNNNNGTC 1 cut(s) 1335
PshBI ATTAAT 1 cut(s) 953
PsiI TTATAA 2 cut(s) 450, 461
Psp6I CCWGG 2 cut(s) 268, 935
PspEI GGTNACC 2 cut(s) 634, 1124
PspGI CCWGG 2 cut(s) 268, 935
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 4 cut(s) 4, 235, 390, 1422
PvuI CGATCG 1 cut(s) 1489
PvuII CAGCTG 1 cut(s) 1586
RsaI GTAC 3 cut(s) 629, 1198, 1404
RsaNI GTAC 3 cut(s) 628, 1197, 1403
RseI CAYNNNNRTG 1 cut(s) 761
SaqAI TTAA 5 cut(s) 45, 776, 953, 1037, 1251
SatI GCNGC 4 cut(s) 186, 906, 1584, 1587
Sau3AI GATC 6 cut(s) 438, 895, 946, 1236, 1341, 1486
Sau96I GGNCC 4 cut(s) 4, 235, 390, 1422
ScrFI CCNGG 2 cut(s) 270, 937
SduI GDGCHC 2 cut(s) 651, 1275
SfaNI GCATC 3 cut(s) 336, 402, 930
SinI GGWCC 2 cut(s) 390, 1422
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 1462
SmoI CTYRAG 1 cut(s) 1462
SsiI CCGC 5 cut(s) 435, 560, 710, 875, 1122
SspI AATATT 1 cut(s) 687
SspMI CTAG 3 cut(s) 470, 530, 1536
StyD4I CCNGG 2 cut(s) 268, 935
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 3 cut(s) 71, 1097, 1130
TaiI ACGT 6 cut(s) 65, 303, 446, 1202, 1272, 1408
TaqI TCGA 3 cut(s) 854, 1165, 1485
Tru1I TTAA 5 cut(s) 45, 776, 953, 1037, 1251
Tru9I TTAA 5 cut(s) 45, 776, 953, 1037, 1251
TscAI CASTG 2 cut(s) 517, 736
TseFI GTSAC 2 cut(s) 496, 1124
TseI GCWGC 4 cut(s) 185, 905, 1583, 1586
Tsp45I GTSAC 2 cut(s) 496, 1124
TspDTI ATGAA 8 cut(s) 153, 183, 853, 885, 900, 1045, 1405, 1581
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 2 cut(s) 517, 736
Van91I CCANNNNNTGG 1 cut(s) 387
VpaK11BI GGWCC 2 cut(s) 390, 1422
VspI ATTAAT 1 cut(s) 953
XapI RAATTY 5 cut(s) 264, 396, 575, 607, 736
XceI RCATGY 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 850
XspI CTAG 3 cut(s) 470, 530, 1536
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.