pycom11g23160

Epidermis-specific secreted glycoprotein EP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016568
Physical Location & Seq
Forward (+)
26184141 .. 26185508
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1368 bp
ATGGCAACTAAAACTTCACATTCAGCTGTTCTCCTACTCTTCTCCCTCATTGCCTTCCTGTTTGCCATTCCCATTCAAGCCCAAGTCCCTGCAAACCAAACCTTCAAATTCATCAACCAAGGCAAATTAGGAGACGGAAACATTGAATATCATGCTACCTACCGCGTGATCCAAACAAATTCCCATACTTTCTATACAAATCCTTTCGGGTTATGCTTCTACAACACCACCCCAGATTCCCACATATTCGCCATCAGAGCCGGTGTTCCCAATGATGAAGTACGGTGGGTCTGGGATGCAAACCGGAATCACCCGGTTCACGAGAAAGCCACTCTCTCTTTTGGTAAAGAAGGAAACTTAGTCCTTGGAGAAGCAGATGGGACTGTTGTTTGGCAAACAAACACAGCCAACAAAGGTGTTACGGGCATCAAGTTACTTGAAAACGGTAACTTGGTCCTCCATGACAAGAATGGAAGATTCATATGGCAGAGCTTTGATTACCCTACCGATACTCTTCTAGCGGGGCAATCAATCAGAACAAATGGTCGTAACAAGCTTGTTAGTCGCAAATCTGATACTGATAGCTCTGATGGGCCATACAGTATGGTTCTTGACAAGACCGGTTTCGCTATGTACTTGAACAACGCTGGCCAGCGTCTTATATATGGCGGGTGGACAGGAACTGACTATGGAAGCACTGTAACCTTTGATTATCAGACTACGAATGGTTTCGAAGATGCCATTGCTTACGAGCTTGTGTTCAATGTAAACCAGGACATTAGTGCTCCACCACCACCACCACAAAGCAAGAGGCGCCTCCTACAAGTTCGTCCCGTTGGCAGCGCAACACAGATCAACCTTAACAAGCTCAACTACAATGCTACGTACTCATTTCTAAGACTCGGGTCCGATGGCAATCTCAAGGCCTATACTTATTATGACAAAGTGAACAGCATGAACTGGGAAGAGAGCTTCGCATTCTTTTCGAGCTATTTCATTAGAGAATGTGCATTGCCATCTAAATGTGGTTCATATGGGTACTGTAGTAGGGGTATGTGTGTGGGGTGCCCCAGCCCGAAAGGGCTTCTGGGTTGGAGCAAGGGCTGTGCAGCCCCAAAGTTGGGACAGTGTAAGAGTGGGGCAAAAGCGAACTACTACAAGGTTGCGGATGTTGAGCACTTTCTGAGTCCTTATTTGGATGGTGGAGATGGTCCAATGAAGGTCGGAGAGTGCAGGGCCAAGTGTGACAAGGACTGTAAGTGCTTAGGGTTCTTTTACAGGGAAGATTCCTCTAAGTGTTTGTTGGCACCAGTGCTTGGGACTCTTATAAAGGACGTGAATACTTCGGCCGGTTACATCAAATATTAA

Protein Analysis

456

Amino Acids

50.32

Weight (kDa)

8.62

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 97 - 185 1.5e-22 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78820 AT1G78830
fragaria_vesca FvH4_3g18370
malus_domestica MD11G1262700.v1.1
prunus_persica Prupe.4G165500_v2.0.a1
pyrus_communis pycom11g23160
rosa_laevigata RLG00000033281
rosa_multiflora Rmu_sc0001523.1_g000002
rosa_roxburghii Rroxscaffold_1G00049250
rosa_rugosa Rorug05G0122300
rosa_samantha Rh5BG214700 Rh5CG238500 Rh5DG219200
rosa_wichuraiana Rw5G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1328
AccB1I GGYRCC 3 cut(s) 813, 1065, 1306
AccB7I CCANNNNNTGG 1 cut(s) 1316
AccII CGCG 1 cut(s) 165
AciI CCGC 4 cut(s) 163, 521, 669, 1166
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 2 cut(s) 649, 1347
AcsI RAATTY 2 cut(s) 107, 178
AcyI GRCGYC 1 cut(s) 814
AfaI GTAC 4 cut(s) 282, 635, 887, 1040
AfiI CCNNNNNNNGG 3 cut(s) 1120, 1121, 1316
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 6 cut(s) 77, 106, 146, 440, 640, 763
AjiI CACGTC 1 cut(s) 1336
AjnI CCWGG 1 cut(s) 771
AluBI AGCT 8 cut(s) 26, 492, 556, 585, 754, 868, 972, 990
AluI AGCT 8 cut(s) 26, 492, 556, 585, 754, 868, 972, 990
Alw21I GWGCWC 2 cut(s) 787, 1179
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 163
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 5 cut(s) 593, 649, 924, 1236, 1347
ApeKI GCWGC 2 cut(s) 840, 1109
ApoI RAATTY 2 cut(s) 107, 178
AsiGI ACCGGT 1 cut(s) 620
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 2 cut(s) 816, 845
AspS9I GGNCC 5 cut(s) 454, 593, 906, 1211, 1236
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 302
AsuII TTCGAA 1 cut(s) 732
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 3 cut(s) 454, 906, 1211
BaeGI GKGCMC 1 cut(s) 1070
BaeI ACNNNNGTAYC 2 cut(s) 1030, 1063
BalI TGGCCA 1 cut(s) 651
BanI GGYRCC 3 cut(s) 813, 1065, 1306
BauI CACGAG 1 cut(s) 320
Bbv12I GWGCWC 2 cut(s) 787, 1179
BbvI GCAGC 2 cut(s) 852, 1121
BccI CCATC 7 cut(s) 260, 371, 584, 905, 1024, 1193, 1202
BcgI CGANNNNNNTGC 2 cut(s) 966, 1000
BciT130I CCWGG 1 cut(s) 773
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 1 cut(s) 1042
BfoI RGCGCY 1 cut(s) 817
BisI GCNGC 2 cut(s) 841, 1110
BlsI GCNGC 2 cut(s) 842, 1111
Bme1390I CCNGG 2 cut(s) 314, 773
Bme18I GGWCC 3 cut(s) 454, 906, 1211
BmeT110I CYCGRG 1 cut(s) 902
BmgBI CACGTC 1 cut(s) 1336
BmgT120I GGNCC 5 cut(s) 454, 593, 906, 1211, 1236
BmiI GGNNCC 4 cut(s) 815, 907, 1067, 1308
BmrFI CCNGG 2 cut(s) 314, 773
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 3 cut(s) 286, 435, 727
BmuI ACTGGG 1 cut(s) 970
BoxI GACNNNNGTC 1 cut(s) 904
Bpu10I CCTNAGC 1 cut(s) 1264
Bpu14I TTCGAA 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 814
BsaJI CCNNGG 2 cut(s) 118, 364
BsaWI WCCGGW 2 cut(s) 303, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 1120, 1121, 1316
Bse118I RCCGGY 3 cut(s) 260, 620, 1349
Bse1I ACTGG 2 cut(s) 965, 1310
Bse3DI GCAATG 3 cut(s) 48, 741, 1010
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 1 cut(s) 773
BseDI CCNNGG 2 cut(s) 118, 364
BseGI GGATG 3 cut(s) 301, 1174, 1204
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 1120, 1121, 1316
BseMI GCAATG 3 cut(s) 48, 741, 1010
BseMII CTCAG 1 cut(s) 1175
BseNI ACTGG 2 cut(s) 965, 1310
BseSI GKGCMC 1 cut(s) 1070
BseX3I CGGCCG 1 cut(s) 1347
BseXI GCAGC 2 cut(s) 852, 1121
BseYI CCCAGC 1 cut(s) 1070
BsgI GTGCAG 2 cut(s) 1128, 1252
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 1350
BshFI GGCC 5 cut(s) 595, 651, 926, 1238, 1349
BshNI GGYRCC 3 cut(s) 813, 1065, 1306
BshTI ACCGGT 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 1350
BsiHKAI GWGCWC 2 cut(s) 787, 1179
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 5 cut(s) 261, 304, 314, 621, 1350
BslFI GGGAC 5 cut(s) 71, 394, 816, 1137, 1333
BslI CCNNNNNNNGG 3 cut(s) 1120, 1121, 1316
BsmAI GTCTC 1 cut(s) 126
BsmBI CGTCTC 1 cut(s) 126
BsmFI GGGAC 5 cut(s) 71, 394, 816, 1137, 1333
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 5 cut(s) 595, 651, 926, 1238, 1349
BsoBI CYCGRG 1 cut(s) 902
Bsp119I TTCGAA 1 cut(s) 732
Bsp1286I GDGCHC 3 cut(s) 787, 1070, 1179
Bsp143I GATC 2 cut(s) 168, 852
BspACI CCGC 4 cut(s) 163, 521, 669, 1166
BspANI GGCC 5 cut(s) 595, 651, 926, 1238, 1349
BspCNI CTCAG 1 cut(s) 1176
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 815, 907, 1067, 1308
BspPI GGATC 1 cut(s) 163
BspT104I TTCGAA 1 cut(s) 732
BspT107I GGYRCC 3 cut(s) 813, 1065, 1306
BsrDI GCAATG 3 cut(s) 48, 741, 1010
BsrFI RCCGGY 3 cut(s) 260, 620, 1349
BsrI ACTGG 2 cut(s) 965, 1310
BssAI RCCGGY 3 cut(s) 260, 620, 1349
BssECI CCNNGG 2 cut(s) 118, 364
BssMI GATC 2 cut(s) 168, 852
BssNI GRCGYC 1 cut(s) 814
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 2 cut(s) 118, 364
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 773
Bst4CI ACNGT 8 cut(s) 285, 385, 446, 602, 700, 1043, 1128, 1256
Bst6I CTCTTC 3 cut(s) 44, 519, 960
BstACI GRCGYC 1 cut(s) 814
BstBAI YACGTR 1 cut(s) 885
BstBI TTCGAA 1 cut(s) 732
BstC8I GCNNGC 2 cut(s) 649, 653
BstDEI CTNAG 5 cut(s) 358, 896, 1184, 1264, 1293
BstF5I GGATG 3 cut(s) 301, 1174, 1204
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 817
BstHHI GCGC 2 cut(s) 816, 845
BstKTI GATC 2 cut(s) 171, 855
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 2 cut(s) 168, 852
BstMCI CGRYCG 1 cut(s) 1350
BstMWI GCNNNNNNNGC 2 cut(s) 257, 813
BstNI CCWGG 1 cut(s) 773
BstPAI GACNNNNGTC 1 cut(s) 904
BstSCI CCNGG 2 cut(s) 312, 771
BstSFI CTRYAG 1 cut(s) 1042
BstSLI GKGCMC 1 cut(s) 1070
BstSNI TACGTA 1 cut(s) 885
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 852, 1121
BstZI CGGCCG 1 cut(s) 1347
BsuRI GGCC 5 cut(s) 595, 651, 926, 1238, 1349
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 3 cut(s) 301, 1174, 1204
BtsIMutI CAGTG 3 cut(s) 696, 1133, 1317
Cac8I GCNNGC 2 cut(s) 649, 653
CaiI CAGNNNCTG 1 cut(s) 683
CfoI GCGC 2 cut(s) 816, 845
Cfr10I RCCGGY 3 cut(s) 260, 620, 1349
Cfr13I GGNCC 5 cut(s) 454, 593, 906, 1211, 1236
CseI GACGC 1 cut(s) 644
Csp6I GTAC 4 cut(s) 281, 634, 886, 1039
CspAI ACCGGT 1 cut(s) 620
CviAII CATG 3 cut(s) 152, 461, 955
CviQI GTAC 4 cut(s) 281, 634, 886, 1039
DdeI CTNAG 5 cut(s) 358, 896, 1184, 1264, 1293
DinI GGCGCC 1 cut(s) 815
DpnI GATC 2 cut(s) 170, 854
DpnII GATC 2 cut(s) 168, 852
EaeI YGGCCR 2 cut(s) 649, 1347
EagI CGGCCG 1 cut(s) 1347
Eam1104I CTCTTC 3 cut(s) 44, 519, 960
EarI CTCTTC 3 cut(s) 44, 519, 960
EclXI CGGCCG 1 cut(s) 1347
Eco105I TACGTA 1 cut(s) 885
Eco130I CCWWGG 2 cut(s) 118, 364
Eco147I AGGCCT 1 cut(s) 926
Eco47I GGWCC 3 cut(s) 454, 906, 1211
Eco52I CGGCCG 1 cut(s) 1347
Eco88I CYCGRG 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 771
EcoT14I CCWWGG 2 cut(s) 118, 364
EgeI GGCGCC 1 cut(s) 815
EheI GGCGCC 1 cut(s) 815
ErhI CCWWGG 2 cut(s) 118, 364
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 3 cut(s) 155, 464, 958
FaqI GGGAC 5 cut(s) 71, 394, 816, 1137, 1333
FatI CATG 3 cut(s) 151, 460, 954
FauI CCCGC 2 cut(s) 514, 662
FauNDI CATATG 2 cut(s) 482, 1033
Fnu4HI GCNGC 2 cut(s) 841, 1110
FokI GGATG 3 cut(s) 308, 1181, 1211
Fsp4HI GCNGC 2 cut(s) 841, 1110
FspBI CTAG 1 cut(s) 518
GlaI GCGC 2 cut(s) 815, 844
GluI GCNGC 2 cut(s) 841, 1110
GsaI CCCAGC 1 cut(s) 1074
HaeII RGCGCY 1 cut(s) 817
HaeIII GGCC 5 cut(s) 595, 651, 926, 1238, 1349
HapII CCGG 5 cut(s) 261, 304, 314, 621, 1350
HgaI GACGC 1 cut(s) 644
HhaI GCGC 2 cut(s) 816, 845
Hin1I GRCGYC 1 cut(s) 814
Hin1II CATG 3 cut(s) 155, 464, 958
Hin6I GCGC 2 cut(s) 814, 843
HinP1I GCGC 2 cut(s) 814, 843
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 7 cut(s) 236, 307, 477, 900, 1186, 1286, 1321
HpaII CCGG 5 cut(s) 261, 304, 314, 621, 1350
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 4 cut(s) 319, 675, 769, 949
Hpy188I TCNGA 8 cut(s) 257, 536, 574, 589, 717, 910, 1185, 1226
Hpy188III TCNNGA 2 cut(s) 320, 611
Hpy8I GTNNAC 4 cut(s) 319, 675, 769, 949
HpyAV CCTTC 4 cut(s) 64, 112, 344, 1213
HpyCH4III ACNGT 8 cut(s) 285, 385, 446, 602, 700, 1043, 1128, 1256
HpyCH4IV ACGT 2 cut(s) 884, 1335
HpyCH4V TGCA 5 cut(s) 92, 299, 1010, 1109, 1233
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 813
HpyF3I CTNAG 5 cut(s) 358, 896, 1184, 1264, 1293
HpySE526I ACGT 2 cut(s) 884, 1335
Hsp92I GRCGYC 1 cut(s) 814
Hsp92II CATG 3 cut(s) 155, 464, 958
HspAI GCGC 2 cut(s) 814, 843
KasI GGCGCC 1 cut(s) 813
Kzo9I GATC 2 cut(s) 168, 852
LmnI GCTCC 2 cut(s) 790, 1095
Lsp1109I GCAGC 2 cut(s) 852, 1121
LweI GCATC 3 cut(s) 286, 435, 727
MaeI CTAG 1 cut(s) 518
MaeII ACGT 2 cut(s) 884, 1335
MaeIII GTNAC 7 cut(s) 418, 432, 446, 548, 700, 1244, 1352
MalI GATC 2 cut(s) 170, 854
MboI GATC 2 cut(s) 168, 852
MboII GAAGA 6 cut(s) 31, 486, 506, 746, 977, 1295
MhlI GDGCHC 3 cut(s) 787, 1070, 1179
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 3 cut(s) 107, 125, 178
MluNI TGGCCA 1 cut(s) 651
Mly113I GGCGCC 1 cut(s) 814
MlyI GAGTC 3 cut(s) 894, 1195, 1315
MmeI TCCRAC 2 cut(s) 1073, 1204
MnlI CCTC 5 cut(s) 56, 467, 804, 827, 1300
Mox20I TGGCCA 1 cut(s) 651
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 651
MseI TTAA 2 cut(s) 861, 1366
MslI CAYNNNNRTG 1 cut(s) 1021
Msp20I TGGCCA 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 5 cut(s) 261, 304, 314, 621, 1350
MspR9I CCNGG 2 cut(s) 314, 773
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 1 cut(s) 773
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 257, 813
NarI GGCGCC 1 cut(s) 814
NciI CCSGG 1 cut(s) 314
NdeI CATATG 2 cut(s) 482, 1033
NdeII GATC 2 cut(s) 168, 852
NlaIII CATG 3 cut(s) 155, 464, 958
NlaIV GGNNCC 4 cut(s) 815, 907, 1067, 1308
NmuCI GTSAC 1 cut(s) 1244
NspV TTCGAA 1 cut(s) 732
PceI AGGCCT 1 cut(s) 926
PctI GAATGC 1 cut(s) 977
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 4 cut(s) 236, 307, 477, 1286
PflMI CCANNNNNTGG 1 cut(s) 1316
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 2 cut(s) 842, 1111
PleI GAGTC 3 cut(s) 894, 1194, 1315
PluTI GGCGCC 1 cut(s) 817
PpsI GAGTC 3 cut(s) 894, 1194, 1315
Ppu21I YACGTR 1 cut(s) 885
PshAI GACNNNNGTC 1 cut(s) 904
PsiI TTATAA 1 cut(s) 1328
Psp6I CCWGG 1 cut(s) 771
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 1 cut(s) 771
PspN4I GGNNCC 4 cut(s) 815, 907, 1067, 1308
PspPI GGNCC 5 cut(s) 454, 593, 906, 1211, 1236
PstNI CAGNNNCTG 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 4 cut(s) 282, 635, 887, 1040
RsaNI GTAC 4 cut(s) 281, 634, 886, 1039
RseI CAYNNNNRTG 1 cut(s) 1021
SaqAI TTAA 2 cut(s) 861, 1366
SatI GCNGC 2 cut(s) 841, 1110
Sau3AI GATC 2 cut(s) 168, 852
Sau96I GGNCC 5 cut(s) 454, 593, 906, 1211, 1236
SchI GAGTC 3 cut(s) 894, 1195, 1315
ScrFI CCNGG 2 cut(s) 314, 773
SduI GDGCHC 3 cut(s) 787, 1070, 1179
SfaNI GCATC 3 cut(s) 286, 435, 727
SfcI CTRYAG 1 cut(s) 1042
SfoI GGCGCC 1 cut(s) 815
SfuI TTCGAA 1 cut(s) 732
SinI GGWCC 3 cut(s) 454, 906, 1211
SmiMI CAYNNNNRTG 1 cut(s) 1021
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SnaBI TACGTA 1 cut(s) 885
Sse9I AATT 3 cut(s) 107, 125, 178
SseBI AGGCCT 1 cut(s) 926
SsiI CCGC 4 cut(s) 163, 521, 669, 1166
SspDI GGCGCC 1 cut(s) 813
SspI AATATT 1 cut(s) 1364
SspMI CTAG 1 cut(s) 518
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 2 cut(s) 312, 771
StyI CCWWGG 2 cut(s) 118, 364
TaaI ACNGT 8 cut(s) 285, 385, 446, 602, 700, 1043, 1128, 1256
TaiI ACGT 2 cut(s) 887, 1338
TaqI TCGA 2 cut(s) 732, 986
TasI AATT 3 cut(s) 107, 125, 178
TatI WGTACW 1 cut(s) 633
TfiI GAWTC 4 cut(s) 236, 307, 477, 1286
Tru1I TTAA 2 cut(s) 861, 1366
Tru9I TTAA 2 cut(s) 861, 1366
TscAI CASTG 3 cut(s) 703, 1133, 1317
TseFI GTSAC 1 cut(s) 1244
TseI GCWGC 2 cut(s) 840, 1109
Tsp45I GTSAC 1 cut(s) 1244
TspDTI ATGAA 7 cut(s) 100, 291, 469, 971, 985, 1020, 1232
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 3 cut(s) 703, 1133, 1317
Van91I CCANNNNNTGG 1 cut(s) 1316
VpaK11BI GGWCC 3 cut(s) 454, 906, 1211
XapI RAATTY 2 cut(s) 107, 178
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.