Rroxscaffold_1G00049250

Epidermis-specific secreted glycoprotein EP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016568
Physical Location & Seq
Forward (+)
69407605 .. 69409920
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049250.1

Sequence Viewer

Length: 1233 bp
ATGGAAGCAAAGAGTGAAAACAGGAATAGGACGAACAATGACATGGTGAAAGTAGAGGAAGATCAGTGCAAAAAGTTTGGGGTTGATGAGAAGATTACTGTTGGAGCAGAGTGCGGTCAGGCCGATCAATGCTTTTCAGAGTGCAAACCAAACATAATGTGCTATCGAGCATCCAAGAGAAGAAAGCCAAGTGTTCTAACTAGAACCTCCAGCAACTACCGCGTGATCCAAACTAAAGCTCACACCTTTTACACACACCCTTTCAGACTATGCTTCTACAATACCACCCCAGATTCTTACATATTTGCCATAAGAGCCGGTGTTCCCAATGATGAGGACCTCATGAGGTGGGTCTGGGACGCAAACCGAAACCACCCGGTGCGCGAGAAAGCCACCCTCTCTCTCGGACGGGATGGGAACTTGATCCTCGCCGAGGCAGACAGGACTGTTGCTTGGCAAACAAACACGGCCAACAAAGGTGTCACAGGAATAAAGTTGCTTCCAGATGGTAACTTGGTTCTTCATGACAAGAATGGAAGATTCATATGGCAAAGCTTTGACTACCCCCATGATACTCTTCTATTGGGGCAGTCGGTCAAAAACATTACTGGTCATAACAAGCTTATTAGTCGTAAATCTGCCACAGATGGCTCTGATGGGCCATACAGTCTTATTCTTGATCGAAGTGGTTTCACTATGTACTTGAACAACTCCGGTCAGCTTCTTACATATGGTGGCTGGACAGACAGTAACTTTGGAAGCACTGTGACATTTGACTATCAAACTACGGATGGCTTTGATGACGTCATAGCTTATGAGCTTTTTCTCAATGTGAACCAAGACATTGATGCTCCACCTCAGAACCGTCGGCGCCTCCTACAAACCCGTCCCATTGGCAGTGCACACCAAATCAACCTCAACAAGCTCAACTACAACGCTAAGTACTCATTCTTAAGACTTGGATCAGATGGCAGTCTCAAGGCCTATACTTATTACAACGAAGTCAGCAACCAAAAATGGGATGAGAGTTTCTCATTCTTTTCGAGTTATTTCGTCAGGGAATGCGCATTGCCATCAAAATGTGGGTCTTATGGGTACTGTAGCAATGGCATGTGTGTGGCATGCCCAAGTCCAGAAGGGCTTCTGGGTTGGAGCAAGGCATGCGCGCCGCCAAAGTTGGCAGCATGTAAGAGCGGAGCTAAGGTGGACTACTACAAAGATTGTGGCGGTTGA

Protein Analysis

410

Amino Acids

46.07

Weight (kDa)

8.53

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 118 - 200 2.1e-19 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78820 AT1G78830
fragaria_vesca FvH4_3g18370
malus_domestica MD11G1262700.v1.1
prunus_persica Prupe.4G165500_v2.0.a1
pyrus_communis pycom11g23160
rosa_laevigata RLG00000033281
rosa_multiflora Rmu_sc0001523.1_g000002
rosa_roxburghii Rroxscaffold_1G00049250
rosa_rugosa Rorug05G0122300
rosa_samantha Rh5BG214700 Rh5CG238500 Rh5DG219200
rosa_wichuraiana Rw5G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 807
Acc16I TGCGCA 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 870
AccBSI CCGCTC 1 cut(s) 1194
AccII CGCG 3 cut(s) 222, 384, 1166
AciI CCGC 5 cut(s) 114, 220, 1169, 1194, 1227
AclWI GGATC 3 cut(s) 220, 418, 970
AcoI YGGCCR 1 cut(s) 468
AcyI GRCGYC 2 cut(s) 804, 871
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 3 cut(s) 701, 944, 1097
AfiI CCNNNNNNNGG 2 cut(s) 433, 1018
AflII CTTAAG 1 cut(s) 952
AgsI TTSAA 1 cut(s) 706
AluBI AGCT 8 cut(s) 239, 555, 622, 721, 812, 820, 925, 1199
AluI AGCT 8 cut(s) 239, 555, 622, 721, 812, 820, 925, 1199
Alw21I GWGCWC 1 cut(s) 904
Alw26I GTCTC 1 cut(s) 980
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 3 cut(s) 220, 418, 970
AoxI GGCC 4 cut(s) 120, 468, 659, 981
ApaLI GTGCAC 1 cut(s) 900
ApeKI GCWGC 1 cut(s) 1181
ArsI GACNNNNNNTTYG 4 cut(s) 737, 769, 1070, 1102
Asp700I GAANNNNTTC 1 cut(s) 1140
AspLEI GCGC 5 cut(s) 384, 873, 1067, 1166, 1168
AspS9I GGNCC 2 cut(s) 337, 659
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 904
BbvI GCAGC 1 cut(s) 1193
BccI CCATC 7 cut(s) 407, 500, 641, 650, 785, 962, 1081
BceAI ACGGC 1 cut(s) 483
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 980
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 1099
BfoI RGCGCY 1 cut(s) 874
BfrI CTTAAG 1 cut(s) 952
BisI GCNGC 2 cut(s) 1169, 1182
BlsI GCNGC 2 cut(s) 1170, 1183
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 337, 659
BmiI GGNNCC 1 cut(s) 872
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 179, 838
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
BpmI CTGGAG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 1200
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 377
BsaHI GRCGYC 2 cut(s) 804, 871
BsaJI CCNNGG 1 cut(s) 432
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 1188, 1218
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 1018
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 2 cut(s) 1067, 1111
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 4 cut(s) 170, 418, 796, 1027
BseLI CCNNNNNNNGG 2 cut(s) 433, 1018
BseMI GCAATG 2 cut(s) 1067, 1111
BseMII CTCAG 1 cut(s) 872
BseNI ACTGG 1 cut(s) 613
BsePI GCGCGC 1 cut(s) 1164
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 1 cut(s) 1193
Bsh1236I CGCG 3 cut(s) 222, 384, 1166
BshFI GGCC 4 cut(s) 122, 470, 661, 983
BshNI GGYRCC 1 cut(s) 870
BsiHKAI GWGCWC 1 cut(s) 904
BsiSI CCGG 3 cut(s) 318, 377, 714
BslFI GGGAC 2 cut(s) 371, 873
BslI CCNNNNNNNGG 2 cut(s) 433, 1018
BsmAI GTCTC 1 cut(s) 980
BsmFI GGGAC 2 cut(s) 371, 873
BsmI GAATGC 1 cut(s) 1067
BsnI GGCC 4 cut(s) 122, 470, 661, 983
Bsp1286I GDGCHC 1 cut(s) 904
Bsp143I GATC 6 cut(s) 61, 124, 225, 423, 679, 962
BspACI CCGC 5 cut(s) 114, 220, 1169, 1194, 1227
BspANI GGCC 4 cut(s) 122, 470, 661, 983
BspCNI CTCAG 1 cut(s) 871
BspFNI CGCG 3 cut(s) 222, 384, 1166
BspHI TCATGA 2 cut(s) 342, 523
BspLI GGNNCC 1 cut(s) 872
BspPI GGATC 3 cut(s) 220, 418, 970
BspT107I GGYRCC 1 cut(s) 870
BspTI CTTAAG 1 cut(s) 952
BsrBI CCGCTC 1 cut(s) 1194
BsrDI GCAATG 2 cut(s) 1067, 1111
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 432
BssHII GCGCGC 1 cut(s) 1164
BssMI GATC 6 cut(s) 61, 124, 225, 423, 679, 962
BssNI GRCGYC 2 cut(s) 804, 871
Bst4CI ACNGT 7 cut(s) 100, 448, 668, 749, 766, 866, 1100
Bst6I CTCTTC 1 cut(s) 582
BstACI GRCGYC 2 cut(s) 804, 871
BstAFI CTTAAG 1 cut(s) 952
BstAPI GCANNNNNTGC 1 cut(s) 1161
BstC8I GCNNGC 3 cut(s) 1123, 1162, 1166
BstDEI CTNAG 3 cut(s) 858, 939, 1200
BstENI CCTNNNNNAGG 1 cut(s) 431
BstF5I GGATG 4 cut(s) 170, 418, 796, 1027
BstFNI CGCG 3 cut(s) 222, 384, 1166
BstH2I RGCGCY 1 cut(s) 874
BstHHI GCGC 5 cut(s) 384, 873, 1067, 1166, 1168
BstKTI GATC 6 cut(s) 64, 127, 228, 426, 682, 965
BstMAI GTCTC 1 cut(s) 980
BstMBI GATC 6 cut(s) 61, 124, 225, 423, 679, 962
BstMWI GCNNNNNNNGC 3 cut(s) 219, 314, 1161
BstNSI RCATGY 4 cut(s) 1114, 1125, 1164, 1188
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 1 cut(s) 1099
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 3 cut(s) 222, 384, 1166
BstV1I GCAGC 1 cut(s) 1193
BsuRI GGCC 4 cut(s) 122, 470, 661, 983
BtsCI GGATG 4 cut(s) 170, 418, 796, 1027
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 3 cut(s) 71, 762, 904
Cac8I GCNNGC 3 cut(s) 1123, 1162, 1166
CciI TCATGA 2 cut(s) 342, 523
CfoI GCGC 5 cut(s) 384, 873, 1067, 1166, 1168
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 337, 659
CseI GACGC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 700, 943, 1096
CspCI CAANNNNNGTGG 1 cut(s) 1204
CviAII CATG 8 cut(s) 43, 343, 524, 569, 1111, 1122, 1161, 1185
CviQI GTAC 3 cut(s) 700, 943, 1096
DdeI CTNAG 3 cut(s) 858, 939, 1200
DinI GGCGCC 1 cut(s) 872
DpnI GATC 6 cut(s) 63, 126, 227, 425, 681, 964
DpnII GATC 6 cut(s) 61, 124, 225, 423, 679, 962
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
Eco147I AGGCCT 1 cut(s) 983
Eco47I GGWCC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 872
EheI GGCGCC 1 cut(s) 872
FaeI CATG 8 cut(s) 46, 346, 527, 572, 1114, 1125, 1164, 1188
FaqI GGGAC 2 cut(s) 371, 873
FatI CATG 8 cut(s) 42, 342, 523, 568, 1110, 1121, 1160, 1184
FauNDI CATATG 2 cut(s) 545, 730
Fnu4HI GCNGC 2 cut(s) 1169, 1182
FokI GGATG 4 cut(s) 157, 425, 803, 1034
Fsp4HI GCNGC 2 cut(s) 1169, 1182
FspAI RTGCGCAY 1 cut(s) 1066
FspBI CTAG 1 cut(s) 201
FspI TGCGCA 1 cut(s) 1066
GlaI GCGC 5 cut(s) 383, 872, 1066, 1165, 1167
GluI GCNGC 2 cut(s) 1169, 1182
GsuI CTGGAG 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 874
HaeIII GGCC 4 cut(s) 122, 470, 661, 983
HapII CCGG 3 cut(s) 318, 377, 714
HgaI GACGC 1 cut(s) 368
HhaI GCGC 5 cut(s) 384, 873, 1067, 1166, 1168
Hin1I GRCGYC 2 cut(s) 804, 871
Hin1II CATG 8 cut(s) 46, 346, 527, 572, 1114, 1125, 1164, 1188
Hin6I GCGC 5 cut(s) 382, 871, 1065, 1164, 1166
HinP1I GCGC 5 cut(s) 382, 871, 1065, 1164, 1166
HindIII AAGCTT 2 cut(s) 553, 620
HinfI GANTC 2 cut(s) 293, 540
HpaII CCGG 3 cut(s) 318, 377, 714
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 3 cut(s) 835, 902, 1207
Hpy188I TCNGA 6 cut(s) 139, 266, 407, 655, 861, 967
Hpy188III TCNNGA 5 cut(s) 343, 503, 524, 677, 1133
Hpy8I GTNNAC 3 cut(s) 835, 902, 1207
Hpy99I CGWCG 1 cut(s) 870
HpyAV CCTTC 1 cut(s) 1130
HpyCH4III ACNGT 7 cut(s) 100, 448, 668, 749, 766, 866, 1100
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 3 cut(s) 69, 144, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 314, 1161
HpyF3I CTNAG 3 cut(s) 858, 939, 1200
HpySE526I ACGT 1 cut(s) 804
Hsp92I GRCGYC 2 cut(s) 804, 871
Hsp92II CATG 8 cut(s) 46, 346, 527, 572, 1114, 1125, 1164, 1188
HspAI GCGC 5 cut(s) 382, 871, 1065, 1164, 1166
KasI GGCGCC 1 cut(s) 870
Kzo9I GATC 6 cut(s) 61, 124, 225, 423, 679, 962
LmnI GCTCC 4 cut(s) 104, 856, 1152, 1196
Lsp1109I GCAGC 1 cut(s) 1193
LweI GCATC 2 cut(s) 179, 838
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 4 cut(s) 481, 509, 749, 766
MalI GATC 6 cut(s) 63, 126, 227, 425, 681, 964
MbiI CCGCTC 1 cut(s) 1194
MboI GATC 6 cut(s) 61, 124, 225, 423, 679, 962
MboII GAAGA 6 cut(s) 71, 103, 192, 512, 549, 569
MhlI GDGCHC 1 cut(s) 904
Mly113I GGCGCC 1 cut(s) 871
MmeI TCCRAC 2 cut(s) 82, 1130
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 1 cut(s) 953
MslI CAYNNNNRTG 2 cut(s) 1078, 1115
MspCI CTTAAG 1 cut(s) 952
MspI CCGG 3 cut(s) 318, 377, 714
MspR9I CCNGG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 1067
MvnI CGCG 3 cut(s) 222, 384, 1166
MwoI GCNNNNNNNGC 3 cut(s) 219, 314, 1161
NarI GGCGCC 1 cut(s) 871
NciI CCSGG 1 cut(s) 377
NdeI CATATG 2 cut(s) 545, 730
NdeII GATC 6 cut(s) 61, 124, 225, 423, 679, 962
NlaIII CATG 8 cut(s) 46, 346, 527, 572, 1114, 1125, 1164, 1188
NlaIV GGNNCC 1 cut(s) 872
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 481, 766
NsbI TGCGCA 1 cut(s) 1066
NspI RCATGY 4 cut(s) 1114, 1125, 1164, 1188
PaeI GCATGC 2 cut(s) 1125, 1164
PagI TCATGA 2 cut(s) 342, 523
PauI GCGCGC 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 983
PctI GAATGC 1 cut(s) 1067
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 2 cut(s) 293, 540
PkrI GCNGC 2 cut(s) 1170, 1183
PluTI GGCGCC 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 872
PspPI GGNCC 2 cut(s) 337, 659
PspPPI RGGWCCY 1 cut(s) 337
PteI GCGCGC 1 cut(s) 1164
RsaI GTAC 3 cut(s) 701, 944, 1097
RsaNI GTAC 3 cut(s) 700, 943, 1096
RseI CAYNNNNRTG 2 cut(s) 1078, 1115
SaqAI TTAA 1 cut(s) 953
SatI GCNGC 2 cut(s) 1169, 1182
Sau3AI GATC 6 cut(s) 61, 124, 225, 423, 679, 962
Sau96I GGNCC 2 cut(s) 337, 659
ScaI AGTACT 1 cut(s) 944
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 904
SfaNI GCATC 2 cut(s) 179, 838
SfcI CTRYAG 1 cut(s) 1099
SfoI GGCGCC 1 cut(s) 872
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 1078, 1115
SmlI CTYRAG 2 cut(s) 952, 977
SmoI CTYRAG 2 cut(s) 952, 977
SphI GCATGC 2 cut(s) 1125, 1164
SseBI AGGCCT 1 cut(s) 983
SsiI CCGC 5 cut(s) 114, 220, 1169, 1194, 1227
SspDI GGCGCC 1 cut(s) 870
SspMI CTAG 1 cut(s) 201
StuI AGGCCT 1 cut(s) 983
StyD4I CCNGG 1 cut(s) 375
TaaI ACNGT 7 cut(s) 100, 448, 668, 749, 766, 866, 1100
TaiI ACGT 1 cut(s) 807
TaqI TCGA 3 cut(s) 166, 682, 1043
TaqII GACCGA 1 cut(s) 583
TatI WGTACW 2 cut(s) 699, 942
TauI GCSGC 1 cut(s) 1171
TfiI GAWTC 2 cut(s) 293, 540
Tru1I TTAA 1 cut(s) 953
Tru9I TTAA 1 cut(s) 953
TscAI CASTG 3 cut(s) 71, 769, 904
TseFI GTSAC 2 cut(s) 481, 766
TseI GCWGC 1 cut(s) 1181
Tsp45I GTSAC 2 cut(s) 481, 766
TspDTI ATGAA 2 cut(s) 512, 532
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 3 cut(s) 71, 769, 904
Vha464I CTTAAG 1 cut(s) 952
VneI GTGCAC 1 cut(s) 900
VpaK11BI GGWCC 1 cut(s) 337
XagI CCTNNNNNAGG 1 cut(s) 431
XceI RCATGY 4 cut(s) 1114, 1125, 1164, 1188
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 1 cut(s) 201
ZraI GACGTC 1 cut(s) 805
ZrmI AGTACT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.