Rw5G019720

Epidermis-specific secreted glycoprotein EP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016568
Physical Location & Seq
Reverse (-)
25664091 .. 25665457
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019720.1

Sequence Viewer

Length: 1332 bp
ATGGCCACTCTAGTTTCTCATGCGCCTTTTCTCCTAGTTCTCTTCCTTCTCGGTAGCTTCTATGTCATTCCCTCTCAAGCCCAAGTCCCTGCAAACCAAACATTCATATTTGTCAACCAAGGCGAACTGGGAGACCGAATAGTCGAATTCGAAGCTAGCTACCGCGTGATCCAAACTAAAGCTCACACCTTTTACACACACCCTTTCAGACTATGCTTCTACAATACCACCCCAGATTCTTACATATTTGCCATAAGAGCCGGTGTTCCCAATGATGAGGACCTCATGAGGTGGGTCTGGGACGCTAACCGAAACCACCCGGTGCACGAGAAAGCTACCCTCTCTCTCGGACGGGATGGGAACTTGATCCTCGCCGAGGCAGACGGGACTGTTGCTTGGCAAACAAACACGGCCAACAAAGGTGTCACAGGAATAAAGTTGCTTCCAGATGGTAACTTGGTTCTTCATGACAAGAATGGAAGATTCATATGGCAAAGCTTTGACTACCCCCATGATACTCTTCTATTGGGGCAGTCGGTCCAAAACATTACTGGTCATAACAAGCTCATTAGTCGTAAATCTGACATAGATGGCTCTGATGGGCCATACAGTCTTATTCTTGATCGAAGTGGTTTCACTATGTACCTGAACAACTCCGGTCAGCTTCTTACATATGGTGGCTGGACAGACAGTAACTTTGGAAGCACTGTGACATTTGACTATCAAACTACGAATGGCTTTGATGATGTCATGGCTTATGAGCTTGTTCTCAATGTGAACCAAGACATTGATGCTCCACCTCAGAACCGTCGGCGCCTCCTACAAACCCGTCCCATTGGCAATGCACATCAAATCAACCTCAACCAGCTCAACTACGACGCTAAGTACTCATTCTTAAGACTTGGATCAGATGGCAGTCTCAAGGCCTATACTTACTACAACAAAGTCAGCAACCAAAAATGGGATGAGAGTTTCTCATTCTTTTCAAGTTATTTCGTCAGGGAATGCGCATTGCCATCAAAATGTGGGTCTTATGGGTACTGTAGCAATGGCATGTGTGTGGCATGCCCAAGTCCAAAAGGGCTTCTGGCATGTAAGAGCGGAGCAAAGGTGGACTACTACAAGATTGTGGCAGTTGAGCACTTTTTGAGTCCCTATTTGGACGGTGGAGATGGACCGATGAAGGTTGAAGAGTGTAGGGCCAAGTGTGATAATGACTGTAAGTGTTTAGGTTTTTTTTACAGGGAAGCCAGCTCAAAGTGTCTGCTAGCACCAGTACTTGGGACTCTCATAAAGGAGGTGAATACATCAGTGGGTTACATCAAGTATTGA

Protein Analysis

443

Amino Acids

49.59

Weight (kDa)

6.37

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 99 - 182 2.3e-19 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78820 AT1G78830
fragaria_vesca FvH4_3g18370
malus_domestica MD11G1262700.v1.1
prunus_persica Prupe.4G165500_v2.0.a1
pyrus_communis pycom11g23160
rosa_laevigata RLG00000033281
rosa_multiflora Rmu_sc0001523.1_g000002
rosa_roxburghii Rroxscaffold_1G00049250
rosa_rugosa Rorug05G0122300
rosa_samantha Rh5BG214700 Rh5CG238500 Rh5DG219200
rosa_wichuraiana Rw5G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
Acc16I TGCGCA 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 813
AccB7I CCANNNNNTGG 1 cut(s) 1280
AccBSI CCGCTC 1 cut(s) 1101
AccII CGCG 1 cut(s) 165
AciI CCGC 2 cut(s) 163, 1101
AclWI GGATC 3 cut(s) 163, 361, 913
AcoI YGGCCR 2 cut(s) 3, 411
AcsI RAATTY 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 814
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 4 cut(s) 644, 887, 1040, 1278
AfiI CCNNNNNNNGG 3 cut(s) 376, 961, 1280
AflII CTTAAG 1 cut(s) 895
AgsI TTSAA 2 cut(s) 987, 1190
Alw21I GWGCWC 2 cut(s) 327, 1143
Alw26I GTCTC 2 cut(s) 126, 923
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 3 cut(s) 163, 361, 913
AoxI GGCC 5 cut(s) 3, 411, 602, 924, 1200
ApaLI GTGCAC 1 cut(s) 323
ApoI RAATTY 1 cut(s) 146
ArsI GACNNNNNNTTYG 4 cut(s) 680, 712, 1013, 1045
AspLEI GCGC 3 cut(s) 25, 816, 1010
AspS9I GGNCC 5 cut(s) 280, 538, 602, 1175, 1200
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 1312
AsuII TTCGAA 1 cut(s) 150
AsuNHI GCTAGC 2 cut(s) 155, 1267
AvaII GGWCC 3 cut(s) 280, 538, 1175
BaeGI GKGCMC 1 cut(s) 327
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 813
BauI CACGAG 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 327, 1143
BccI CCATC 7 cut(s) 350, 443, 584, 593, 905, 1024, 1166
BceAI ACGGC 1 cut(s) 426
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 126, 923
BfaI CTAG 4 cut(s) 11, 35, 156, 1268
BfmI CTRYAG 1 cut(s) 1042
BfoI RGCGCY 1 cut(s) 817
BfrI CTTAAG 1 cut(s) 895
BmcAI AGTACT 2 cut(s) 887, 1278
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 3 cut(s) 280, 538, 1175
BmgT120I GGNCC 5 cut(s) 280, 538, 602, 1175, 1200
BmiI GGNNCC 1 cut(s) 815
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 781
BmtI GCTAGC 2 cut(s) 159, 1271
BmuI ACTGGG 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 959, 991
Bpu14I TTCGAA 1 cut(s) 150
BpuEI CTTGAG 2 cut(s) 60, 905
BpuMI CCSGG 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 814
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 118, 375
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 961, 1280
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 132, 556, 1274
Bse3DI GCAATG 3 cut(s) 847, 1010, 1054
BseDI CCNNGG 2 cut(s) 118, 375
BseGI GGATG 2 cut(s) 361, 970
BseLI CCNNNNNNNGG 3 cut(s) 376, 961, 1280
BseMI GCAATG 3 cut(s) 847, 1010, 1054
BseMII CTCAG 1 cut(s) 815
BseNI ACTGG 3 cut(s) 132, 556, 1274
BseSI GKGCMC 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 5 cut(s) 5, 413, 604, 926, 1202
BshNI GGYRCC 1 cut(s) 813
BsiHKAI GWGCWC 2 cut(s) 327, 1143
BsiSI CCGG 3 cut(s) 261, 320, 657
BslFI GGGAC 6 cut(s) 71, 314, 400, 816, 1137, 1297
BslI CCNNNNNNNGG 3 cut(s) 376, 961, 1280
BsmAI GTCTC 2 cut(s) 126, 923
BsmFI GGGAC 6 cut(s) 71, 314, 400, 816, 1137, 1297
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 5 cut(s) 5, 413, 604, 926, 1202
Bso31I GGTCTC 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 327, 1143
Bsp143I GATC 4 cut(s) 168, 366, 622, 905
BspACI CCGC 2 cut(s) 163, 1101
BspANI GGCC 5 cut(s) 5, 413, 604, 926, 1202
BspCNI CTCAG 1 cut(s) 814
BspFNI CGCG 1 cut(s) 165
BspHI TCATGA 2 cut(s) 285, 466
BspLI GGNNCC 1 cut(s) 815
BspOI GCTAGC 2 cut(s) 159, 1271
BspPI GGATC 3 cut(s) 163, 361, 913
BspT104I TTCGAA 1 cut(s) 150
BspT107I GGYRCC 1 cut(s) 813
BspTI CTTAAG 1 cut(s) 895
BspTNI GGTCTC 1 cut(s) 126
BsrBI CCGCTC 1 cut(s) 1101
BsrDI GCAATG 3 cut(s) 847, 1010, 1054
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 3 cut(s) 132, 556, 1274
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 2 cut(s) 118, 375
BssMI GATC 4 cut(s) 168, 366, 622, 905
BssNI GRCGYC 1 cut(s) 814
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 326
Bst4CI ACNGT 8 cut(s) 391, 611, 692, 709, 809, 1043, 1166, 1220
Bst6I CTCTTC 3 cut(s) 47, 525, 1185
BstACI GRCGYC 1 cut(s) 814
BstAFI CTTAAG 1 cut(s) 895
BstBI TTCGAA 1 cut(s) 150
BstC8I GCNNGC 4 cut(s) 157, 1066, 1252, 1269
BstDEI CTNAG 2 cut(s) 801, 882
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 361, 970
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 817
BstHHI GCGC 3 cut(s) 25, 816, 1010
BstKTI GATC 4 cut(s) 171, 369, 625, 908
BstMAI GTCTC 2 cut(s) 126, 923
BstMBI GATC 4 cut(s) 168, 366, 622, 905
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNSI RCATGY 3 cut(s) 1057, 1068, 1095
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 1042
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 1 cut(s) 165
BsuRI GGCC 5 cut(s) 5, 413, 604, 926, 1202
BtsCI GGATG 2 cut(s) 361, 970
BtsIMutI CAGTG 2 cut(s) 705, 1317
Cac8I GCNNGC 4 cut(s) 157, 1066, 1252, 1269
CciI TCATGA 2 cut(s) 285, 466
CfoI GCGC 3 cut(s) 25, 816, 1010
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 5 cut(s) 280, 538, 602, 1175, 1200
CseI GACGC 2 cut(s) 311, 887
Csp6I GTAC 4 cut(s) 643, 886, 1039, 1277
CviAII CATG 8 cut(s) 20, 286, 467, 512, 751, 1054, 1065, 1092
CviQI GTAC 4 cut(s) 643, 886, 1039, 1277
DdeI CTNAG 2 cut(s) 801, 882
DinI GGCGCC 1 cut(s) 815
DpnI GATC 4 cut(s) 170, 368, 624, 907
DpnII GATC 4 cut(s) 168, 366, 622, 905
DraIII CACNNNGTG 1 cut(s) 322
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 2 cut(s) 3, 411
Eam1104I CTCTTC 3 cut(s) 47, 525, 1185
EarI CTCTTC 3 cut(s) 47, 525, 1185
Eco130I CCWWGG 1 cut(s) 118
Eco147I AGGCCT 1 cut(s) 926
Eco31I GGTCTC 1 cut(s) 126
Eco47I GGWCC 3 cut(s) 280, 538, 1175
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 118
EgeI GGCGCC 1 cut(s) 815
EheI GGCGCC 1 cut(s) 815
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 8 cut(s) 23, 289, 470, 515, 754, 1057, 1068, 1095
FaqI GGGAC 6 cut(s) 71, 314, 400, 816, 1137, 1297
FatI CATG 8 cut(s) 19, 285, 466, 511, 750, 1053, 1064, 1091
FauNDI CATATG 2 cut(s) 488, 673
FokI GGATG 2 cut(s) 368, 977
FspAI RTGCGCAY 1 cut(s) 1009
FspBI CTAG 4 cut(s) 11, 35, 156, 1268
FspI TGCGCA 1 cut(s) 1009
GlaI GCGC 3 cut(s) 24, 815, 1009
HaeII RGCGCY 1 cut(s) 817
HaeIII GGCC 5 cut(s) 5, 413, 604, 926, 1202
HapII CCGG 3 cut(s) 261, 320, 657
HgaI GACGC 2 cut(s) 311, 887
HhaI GCGC 3 cut(s) 25, 816, 1010
Hin1I GRCGYC 1 cut(s) 814
Hin1II CATG 8 cut(s) 23, 289, 470, 515, 754, 1057, 1068, 1095
Hin6I GCGC 3 cut(s) 23, 814, 1008
HinP1I GCGC 3 cut(s) 23, 814, 1008
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 4 cut(s) 236, 483, 1150, 1285
HpaII CCGG 3 cut(s) 261, 320, 657
HphI GGTGA 1 cut(s) 1312
Hpy166II GTNNAC 4 cut(s) 115, 325, 778, 1114
Hpy188I TCNGA 6 cut(s) 209, 350, 583, 598, 804, 910
Hpy188III TCNNGA 4 cut(s) 286, 446, 467, 620
Hpy8I GTNNAC 4 cut(s) 115, 325, 778, 1114
Hpy99I CGWCG 2 cut(s) 813, 881
HpyAV CCTTC 2 cut(s) 56, 1177
HpyCH4III ACNGT 8 cut(s) 391, 611, 692, 709, 809, 1043, 1166, 1220
HpyCH4V TGCA 3 cut(s) 92, 325, 845
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 2 cut(s) 801, 882
Hsp92I GRCGYC 1 cut(s) 814
Hsp92II CATG 8 cut(s) 23, 289, 470, 515, 754, 1057, 1068, 1095
HspAI GCGC 3 cut(s) 23, 814, 1008
KasI GGCGCC 1 cut(s) 813
Kzo9I GATC 4 cut(s) 168, 366, 622, 905
LmnI GCTCC 2 cut(s) 799, 1103
LweI GCATC 1 cut(s) 781
MaeI CTAG 4 cut(s) 11, 35, 156, 1268
MaeIII GTNAC 5 cut(s) 424, 452, 692, 709, 1316
MalI GATC 4 cut(s) 170, 368, 624, 907
MbiI CCGCTC 1 cut(s) 1101
MboI GATC 4 cut(s) 168, 366, 622, 905
MboII GAAGA 5 cut(s) 34, 455, 492, 512, 1202
MhlI GDGCHC 2 cut(s) 327, 1143
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 814
MlyI GAGTC 2 cut(s) 1159, 1279
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 896
MslI CAYNNNNRTG 2 cut(s) 1021, 1058
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 895
MspI CCGG 3 cut(s) 261, 320, 657
MspR9I CCNGG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 1010
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 1 cut(s) 257
NarI GGCGCC 1 cut(s) 814
NciI CCSGG 1 cut(s) 320
NdeI CATATG 2 cut(s) 488, 673
NdeII GATC 4 cut(s) 168, 366, 622, 905
NheI GCTAGC 2 cut(s) 155, 1267
NlaIII CATG 8 cut(s) 23, 289, 470, 515, 754, 1057, 1068, 1095
NlaIV GGNNCC 1 cut(s) 815
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 424, 709
NsbI TGCGCA 1 cut(s) 1009
NspI RCATGY 3 cut(s) 1057, 1068, 1095
NspV TTCGAA 1 cut(s) 150
PaeI GCATGC 1 cut(s) 1068
PagI TCATGA 2 cut(s) 285, 466
PceI AGGCCT 1 cut(s) 926
PctI GAATGC 1 cut(s) 1010
PfeI GAWTC 2 cut(s) 236, 483
PflMI CCANNNNNTGG 1 cut(s) 1280
PleI GAGTC 2 cut(s) 1158, 1279
PluTI GGCGCC 1 cut(s) 817
PpsI GAGTC 2 cut(s) 1158, 1279
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 815
PspPI GGNCC 5 cut(s) 280, 538, 602, 1175, 1200
PspPPI RGGWCCY 1 cut(s) 280
RsaI GTAC 4 cut(s) 644, 887, 1040, 1278
RsaNI GTAC 4 cut(s) 643, 886, 1039, 1277
RseI CAYNNNNRTG 2 cut(s) 1021, 1058
SaqAI TTAA 1 cut(s) 896
Sau3AI GATC 4 cut(s) 168, 366, 622, 905
Sau96I GGNCC 5 cut(s) 280, 538, 602, 1175, 1200
ScaI AGTACT 2 cut(s) 887, 1278
SchI GAGTC 2 cut(s) 1159, 1279
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 2 cut(s) 327, 1143
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 1 cut(s) 1042
SfoI GGCGCC 1 cut(s) 815
SfuI TTCGAA 1 cut(s) 150
SinI GGWCC 3 cut(s) 280, 538, 1175
SmiMI CAYNNNNRTG 2 cut(s) 1021, 1058
SmlI CTYRAG 3 cut(s) 75, 895, 920
SmoI CTYRAG 3 cut(s) 75, 895, 920
SphI GCATGC 1 cut(s) 1068
Sse9I AATT 1 cut(s) 146
SseBI AGGCCT 1 cut(s) 926
SsiI CCGC 2 cut(s) 163, 1101
SspDI GGCGCC 1 cut(s) 813
SspMI CTAG 4 cut(s) 11, 35, 156, 1268
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 8 cut(s) 391, 611, 692, 709, 809, 1043, 1166, 1220
TaqI TCGA 3 cut(s) 144, 150, 625
TaqII GACCGA 3 cut(s) 150, 526, 1192
TasI AATT 1 cut(s) 146
TatI WGTACW 2 cut(s) 885, 1276
TfiI GAWTC 2 cut(s) 236, 483
Tru1I TTAA 1 cut(s) 896
Tru9I TTAA 1 cut(s) 896
TscAI CASTG 2 cut(s) 712, 1317
TseFI GTSAC 2 cut(s) 424, 709
Tsp45I GTSAC 2 cut(s) 424, 709
TspDTI ATGAA 4 cut(s) 94, 455, 475, 1196
TspRI CASTG 2 cut(s) 712, 1317
Van91I CCANNNNNTGG 1 cut(s) 1280
Vha464I CTTAAG 1 cut(s) 895
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 3 cut(s) 280, 538, 1175
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 146
XceI RCATGY 3 cut(s) 1057, 1068, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XspI CTAG 4 cut(s) 11, 35, 156, 1268
ZrmI AGTACT 2 cut(s) 887, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.