Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

27691237 .. 27695966

Gene Structure 4730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGCCGTCGTTCGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAAGCCTTCCATTTCCGGTTAGTAACAGTAAGAGATTTCTCGGCCTCTGAGACGCGGAGAGTAGAGAAGATTCTGATAGCGAACAGAGGCGAAATAGCCTGCAGGATCATGCGGACCGCCAAGCGACTCGGGATTCAAACAGTCGCCGTTTACAGCGACGCCGATAGGCATTCGCTTCACGTCAAATCGGCCGACGAGGCCGTGCATATTGGACCTCCACCGGCACGGCTCAGCTACCTCAACGCCTCCTCGATTCTCGACGCCGCCGTTCGTACTGGCGCTCAGGCTATCCATCCTGGCTATGGCTTTCTGTCGGAGAGTGCTGACTTTGCTCAACTTTGTGAAGATAAAGGTCTTACATTTATTGGGCCTCCGGCATCTGCCATTCGCGACATGGGTGATAAAAGTGCTTCAAAGAGAATAATGGGCGCGGCAGGGGTGCCGCTTGTGCCTGGATATCATGGTAACGATCAAGATATTGATCTAATGAAGTTAGAAGCTGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGAGGAAAGGGTATGAGGATTGTGCAGAGTCCTGATGAATTTGTCGAAGCTTTTTTGGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCATTAGCACAATATTGTTGGAGAAGTATATTACACGGCCAAGGCACATAGAAGTTCAGATCTTTGGGGACAAACATGGGAATGTTCTACATTTATATGAAAGAGATTGCAGCGTCCAGAGAAGACACCAGAAAATAATTGAAGAAGCCCCAGCTCCAAATGTTTCTAATGACTTTCGATCTCACTTGGGTCAAGCTGCTGTATCTGCTGCCAAGGCAGTTGGTTATCACAATGCTGGCACTGTGGAGTTTATAGTTGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAACACTCGACTTCAGGTTGAGCATCCTGTGACTGAGATGATCGTCGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTTGCAAACGGAGAACATCTTCCTATTAGTCAGTCACAGATCCCCTTATCAGGTCATGCTTTCGAAGCCCGAATATATGCTGAAAATGTACCGAAAGGATTCCTTCCAGCAACTGGAGTCCTTCATCACTATCATCACGTTCCAGTCTCACCAACAGTTAGAGTTGAGACTGGAGTTGAACGTGGAGACACTGTTAGCATGCATTATGATCCTATGATTGCTAAGCTTGTAGTATGGGGAGAAAATCGCACTGCAGCATTGGTGAAATTGAAGGATTGTTTGTCAAAGTTTCAGGTTGCAGGTTTACCAACCAACATCAATTTTCTGCTAAAACTTGCTAACCATCAGGCATTTGAAAATGGTGATGTGGAAACCCATTTTATTGAACGCTTTAAAGATGACCTCTTTGTCGACCCTAGTAATTCACTGCTGGTGGACAAAGTGGCTGGTGCTGCTAGATTTGGTGCTACACTCGCAGCTGCGTGTCTCATTGAAAAGGAAAATTATGAATTTAGAGAAAATCTTCCTGGTAGCAAAGGCATAAACTCCATATGGTATTCTTCTCCCCCCTTCAGAGTCCATCATTGTGCTAGACATACTATGGAGCTTGAGTGGGAAAATGAATACGATAGCAGTGGCTCAAAGTTACTGAAGATTTCAACCACTTATAAAGAAGATGGGAGCTATCTGGTTGAGGCAGAAGAAGACAGTTCCCAATGTGTGGAGGTTAAAGCAACTTGCATAGGCAACCACGATTTCCGAGTTGAAGCTGATGGTGTAATCACGGAGGTTTGCTTAGCTGTTTACTCCAAGGATCAAACCAAGCTCATTCATATATGGCATGGGTCACATCATCATCATTTCAAGCAGAAAATTGGTCTTGAATTGTATGATGATGATCGATCTCGAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

653

Amino Acids

72.3

Weight (kDa)

6.37

Isoelectric Point (pI)

35.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	35 - 143	1.2e-45	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	148 - 356	1.8e-74	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	175 - 325	5.1e-10	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	370 - 478	9e-36	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	489 - 651	1.9e-76	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1713
AasI	GACNNNNNNGTC	1 cut(s)	1451
Acc36I	ACCTGC	1 cut(s)	1334
AccB1I	GGYRCC	1 cut(s)	481
AccB7I	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
AccI	GTMKAC	1 cut(s)	1455
AccII	CGCG	3 cut(s)	97, 432, 473
AciI	CCGC	7 cut(s)	25, 97, 154, 159, 306, 473, 485
AclWI	GGATC	4 cut(s)	155, 1078, 1247, 1866
AcoI	YGGCCR	3 cut(s)	231, 713, 1015
AcsI	RAATTY	3 cut(s)	626, 1038, 1553
AcuI	CTGAAG	3 cut(s)	965, 1600, 1715
AcyI	GRCGYC	2 cut(s)	201, 303
AdeI	CACNNNGTG	1 cut(s)	656
AfaI	GTAC	2 cut(s)	316, 1134
AfiI	CCNNNNNNNGG	5 cut(s)	344, 581, 1094, 1157, 1765
AjiI	CACGTC	1 cut(s)	223
AjnI	CCWGG	3 cut(s)	337, 493, 1570
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	6 cut(s)	86, 946, 1195, 1205, 1224, 1535
AlwI	GGATC	4 cut(s)	155, 1078, 1247, 1866
AlwNI	CAGNNNCTG	1 cut(s)	1157
Ama87I	CYCGRG	1 cut(s)	170
AoxI	GGCC	7 cut(s)	84, 231, 240, 410, 713, 946, 1015
ApeKI	GCWGC	9 cut(s)	662, 665, 786, 872, 884, 1298, 1496, 1520, 1523
ApoI	RAATTY	3 cut(s)	626, 1038, 1553
Asp700I	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
AspLEI	GCGC	2 cut(s)	323, 473
AspS9I	GGNCC	3 cut(s)	156, 254, 410
AsuHPI	GGTGA	4 cut(s)	452, 1185, 1318, 1418
AsuII	TTCGAA	1 cut(s)	1107
AvaI	CYCGRG	1 cut(s)	170
AvaII	GGWCC	2 cut(s)	156, 254
BanI	GGYRCC	1 cut(s)	481
BbsI	GAAGAC	2 cut(s)	805, 1755
Bbv12I	GWGCWC	1 cut(s)	652
BbvI	GCAGC	9 cut(s)	649, 652, 798, 859, 871, 1310, 1483, 1510, 1532
BccI	CCATC	5 cut(s)	342, 1395, 1632, 1715, 1811
BceAI	ACGGC	5 cut(s)	173, 227, 284, 293, 728
BciT130I	CCWGG	3 cut(s)	339, 495, 1572
BcoDI	GTCTC	6 cut(s)	86, 946, 1195, 1205, 1224, 1535
BfaI	CTAG	3 cut(s)	1461, 1500, 1635
BfmI	CTRYAG	2 cut(s)	142, 1296
BfoI	RGCGCY	1 cut(s)	324
BfuAI	ACCTGC	1 cut(s)	1334
BglI	GCCNNNNNGGC	1 cut(s)	239
BglII	AGATCT	2 cut(s)	735, 1021
BlpI	GCTNAGC	3 cut(s)	272, 1266, 1840
Bme1390I	CCNGG	3 cut(s)	339, 495, 1572
Bme18I	GGWCC	2 cut(s)	156, 254
BmeT110I	CYCGRG	1 cut(s)	170
BmgBI	CACGTC	1 cut(s)	223
BmgT120I	GGNCC	3 cut(s)	156, 254, 410
BmiI	GGNNCC	1 cut(s)	483
BmrFI	CCNGG	3 cut(s)	339, 495, 1572
BmsI	GCATC	2 cut(s)	428, 1000
BpiI	GAAGAC	2 cut(s)	805, 1755
BpmI	CTGGAG	2 cut(s)	1179, 1236
Bpu10I	CCTNAGC	1 cut(s)	324
Bpu1102I	GCTNAGC	3 cut(s)	272, 1266, 1840
Bpu14I	TTCGAA	1 cut(s)	1107
BpuEI	CTTGAG	1 cut(s)	1673
Bsa29I	ATCGAT	1 cut(s)	1945
BsaBI	GATNNNNATC	2 cut(s)	522, 1941
BsaHI	GRCGYC	2 cut(s)	201, 303
BsaJI	CCNNGG	3 cut(s)	716, 888, 1854
BsaWI	WCCGGW	1 cut(s)	57
BsaXI	ACNNNNNCTCC	4 cut(s)	1221, 1251, 1640, 1670
Bsc4I	CCNNNNNNNGG	5 cut(s)	344, 581, 1094, 1157, 1765
Bse118I	RCCGGY	1 cut(s)	262
Bse1I	ACTGG	4 cut(s)	322, 1162, 1187, 1219
Bse8I	GATNNNNATC	2 cut(s)	522, 1941
BseBI	CCWGG	3 cut(s)	339, 495, 1572
BseCI	ATCGAT	1 cut(s)	1945
BseDI	CCNNGG	3 cut(s)	716, 888, 1854
BseGI	GGATG	2 cut(s)	334, 991
BseJI	GATNNNNATC	2 cut(s)	522, 1941
BseLI	CCNNNNNNNGG	5 cut(s)	344, 581, 1094, 1157, 1765
BseMII	CTCAG	5 cut(s)	81, 286, 338, 957, 993
BseNI	ACTGG	4 cut(s)	322, 1162, 1187, 1219
BseRI	GAGGAG	1 cut(s)	280
BseX3I	CGGCCG	1 cut(s)	231
BseXI	GCAGC	9 cut(s)	649, 652, 798, 859, 871, 1310, 1483, 1510, 1532
BseYI	CCCAGC	1 cut(s)	826
BsgI	GTGCAG	1 cut(s)	632
Bsh1236I	CGCG	3 cut(s)	97, 432, 473
Bsh1285I	CGRYCG	1 cut(s)	234
BshFI	GGCC	7 cut(s)	86, 233, 242, 412, 715, 948, 1017
BshNI	GGYRCC	1 cut(s)	481
BshVI	ATCGAT	1 cut(s)	1945
BsiEI	CGRYCG	1 cut(s)	234
BsiHKAI	GWGCWC	1 cut(s)	652
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	5 cut(s)	28, 39, 58, 263, 416
BslFI	GGGAC	1 cut(s)	758
BslI	CCNNNNNNNGG	5 cut(s)	344, 581, 1094, 1157, 1765
BsmAI	GTCTC	6 cut(s)	86, 946, 1195, 1205, 1224, 1535
BsmBI	CGTCTC	1 cut(s)	86
BsmFI	GGGAC	1 cut(s)	758
BsmI	GAATGC	1 cut(s)	211
BsnI	GGCC	7 cut(s)	86, 233, 242, 412, 715, 948, 1017
BsoBI	CYCGRG	1 cut(s)	170
Bsp119I	TTCGAA	1 cut(s)	1107
Bsp1286I	GDGCHC	1 cut(s)	652
Bsp1720I	GCTNAGC	3 cut(s)	272, 1266, 1840
Bsp68I	TCGCGA	1 cut(s)	432
BspACI	CCGC	7 cut(s)	25, 97, 154, 159, 306, 473, 485
BspANI	GGCC	7 cut(s)	86, 233, 242, 412, 715, 948, 1017
BspCNI	CTCAG	5 cut(s)	82, 285, 337, 956, 994
BspDI	ATCGAT	1 cut(s)	1945
BspFNI	CGCG	3 cut(s)	97, 432, 473
BspLI	GGNNCC	1 cut(s)	483
BspMAI	CTGCAG	2 cut(s)	146, 1300
BspMI	ACCTGC	1 cut(s)	1334
BspPI	GGATC	4 cut(s)	155, 1078, 1247, 1866
BspT104I	TTCGAA	1 cut(s)	1107
BspT107I	GGYRCC	1 cut(s)	481
BsrFI	RCCGGY	1 cut(s)	262
BsrI	ACTGG	4 cut(s)	322, 1162, 1187, 1219
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	3 cut(s)	716, 888, 1854
BssNI	GRCGYC	2 cut(s)	201, 303
BssT1I	CCWWGG	3 cut(s)	716, 888, 1854
Bst2UI	CCWGG	3 cut(s)	339, 495, 1572
Bst4CI	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
BstACI	GRCGYC	2 cut(s)	201, 303
BstBI	TTCGAA	1 cut(s)	1107
BstC8I	GCNNGC	3 cut(s)	142, 913, 1244
BstDEI	CTNAG	7 cut(s)	90, 272, 324, 943, 1002, 1266, 1840
BstF5I	GGATG	2 cut(s)	334, 991
BstFNI	CGCG	3 cut(s)	97, 432, 473
BstH2I	RGCGCY	1 cut(s)	324
BstHHI	GCGC	2 cut(s)	323, 473
BstMAI	GTCTC	6 cut(s)	86, 946, 1195, 1205, 1224, 1535
BstMCI	CGRYCG	1 cut(s)	234
BstMWI	GCNNNNNNNGC	8 cut(s)	239, 371, 490, 881, 890, 1109, 1496, 1517
BstNI	CCWGG	3 cut(s)	339, 495, 1572
BstNSI	RCATGY	1 cut(s)	1246
BstSCI	CCNGG	3 cut(s)	337, 493, 1570
BstSFI	CTRYAG	2 cut(s)	142, 1296
BstUI	CGCG	3 cut(s)	97, 432, 473
BstV1I	GCAGC	9 cut(s)	649, 652, 798, 859, 871, 1310, 1483, 1510, 1532
BstV2I	GAAGAC	2 cut(s)	805, 1755
BstX2I	RGATCY	3 cut(s)	735, 1021, 1083
BstYI	RGATCY	3 cut(s)	735, 1021, 1083
BstZI	CGGCCG	1 cut(s)	231
Bsu15I	ATCGAT	1 cut(s)	1945
BsuRI	GGCC	7 cut(s)	86, 233, 242, 412, 715, 948, 1017
BsuTUI	ATCGAT	1 cut(s)	1945
BtrI	CACGTC	1 cut(s)	223
BtsCI	GGATG	2 cut(s)	334, 991
BtsI	GCAGTG	3 cut(s)	1293, 1469, 1684
BtsIMutI	CAGTG	5 cut(s)	915, 1233, 1293, 1469, 1684
BtuMI	TCGCGA	1 cut(s)	432
BveI	ACCTGC	1 cut(s)	1334
Cac8I	GCNNGC	3 cut(s)	142, 913, 1244
CaiI	CAGNNNCTG	1 cut(s)	1157
CfoI	GCGC	2 cut(s)	323, 473
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	3 cut(s)	156, 254, 410
ClaI	ATCGAT	1 cut(s)	1945
CpoI	CGGWCCG	1 cut(s)	156
CseI	GACGC	4 cut(s)	103, 209, 311, 778
Csp6I	GTAC	2 cut(s)	315, 1133
CspI	CGGWCCG	1 cut(s)	156
CviAII	CATG	9 cut(s)	151, 436, 503, 581, 752, 961, 1100, 1243, 1886
CviQI	GTAC	2 cut(s)	315, 1133
DdeI	CTNAG	7 cut(s)	90, 272, 324, 943, 1002, 1266, 1840
DraI	TTTAAA	1 cut(s)	1438
DraIII	CACNNNGTG	1 cut(s)	656
DrdI	GACNNNNNNGTC	1 cut(s)	1451
DseDI	GACNNNNNNGTC	1 cut(s)	1451
EaeI	YGGCCR	3 cut(s)	231, 713, 1015
EagI	CGGCCG	1 cut(s)	231
EciI	GGCGGA	1 cut(s)	14
EclXI	CGGCCG	1 cut(s)	231
Eco130I	CCWWGG	3 cut(s)	716, 888, 1854
Eco32I	GATATC	2 cut(s)	500, 557
Eco47I	GGWCC	2 cut(s)	156, 254
Eco52I	CGGCCG	1 cut(s)	231
Eco57I	CTGAAG	3 cut(s)	965, 1600, 1715
Eco88I	CYCGRG	1 cut(s)	170
EcoRII	CCWGG	3 cut(s)	337, 493, 1570
EcoRV	GATATC	2 cut(s)	500, 557
EcoT14I	CCWWGG	3 cut(s)	716, 888, 1854
EcoT22I	ATGCAT	1 cut(s)	1248
ErhI	CCWWGG	3 cut(s)	716, 888, 1854
Esp3I	CGTCTC	1 cut(s)	86
FaeI	CATG	9 cut(s)	154, 439, 506, 584, 755, 964, 1103, 1246, 1889
FalI	AAGNNNNNCTT	2 cut(s)	1131, 1163
FaqI	GGGAC	1 cut(s)	758
FatI	CATG	9 cut(s)	150, 435, 502, 580, 751, 960, 1099, 1242, 1885
FauNDI	CATATG	1 cut(s)	1595
FblI	GTMKAC	1 cut(s)	1455
FokI	GGATG	2 cut(s)	321, 978
FspBI	CTAG	3 cut(s)	1461, 1500, 1635
GlaI	GCGC	2 cut(s)	322, 472
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	2 cut(s)	1179, 1236
HaeII	RGCGCY	1 cut(s)	324
HaeIII	GGCC	7 cut(s)	86, 233, 242, 412, 715, 948, 1017
HapII	CCGG	5 cut(s)	28, 39, 58, 263, 416
HgaI	GACGC	4 cut(s)	103, 209, 311, 778
HhaI	GCGC	2 cut(s)	323, 473
Hin1I	GRCGYC	2 cut(s)	201, 303
Hin1II	CATG	9 cut(s)	154, 439, 506, 584, 755, 964, 1103, 1246, 1889
Hin6I	GCGC	2 cut(s)	321, 471
HinP1I	GCGC	2 cut(s)	321, 471
HincII	GTYRAC	1 cut(s)	1456
HindII	GTYRAC	1 cut(s)	1456
HindIII	AAGCTT	2 cut(s)	636, 1268
HinfI	GANTC	8 cut(s)	112, 168, 175, 295, 616, 1143, 1161, 1620
HpaII	CCGG	5 cut(s)	28, 39, 58, 263, 416
HphI	GGTGA	4 cut(s)	452, 1185, 1318, 1418
Hpy166II	GTNNAC	5 cut(s)	193, 1349, 1456, 1480, 1849
Hpy188I	TCNGA	6 cut(s)	91, 117, 358, 735, 1619, 1805
Hpy188III	TCNNGA	8 cut(s)	172, 299, 431, 515, 620, 793, 1925, 1950
Hpy8I	GTNNAC	5 cut(s)	193, 1349, 1456, 1480, 1849
Hpy99I	CGWCG	5 cut(s)	10, 203, 239, 305, 1016
HpyAV	CCTTC	5 cut(s)	58, 1157, 1175, 1309, 1624
HpyCH4III	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
HpyCH4IV	ACGT	4 cut(s)	222, 655, 1182, 1225
HpyCH4V	TGCA	9 cut(s)	144, 247, 613, 786, 1049, 1246, 1298, 1343, 1785
HpyF10VI	GCNNNNNNNGC	8 cut(s)	239, 371, 490, 881, 890, 1109, 1496, 1517
HpyF3I	CTNAG	7 cut(s)	90, 272, 324, 943, 1002, 1266, 1840
HpySE526I	ACGT	4 cut(s)	222, 655, 1182, 1225
Hsp92I	GRCGYC	2 cut(s)	201, 303
Hsp92II	CATG	9 cut(s)	154, 439, 506, 584, 755, 964, 1103, 1246, 1889
HspAI	GCGC	2 cut(s)	321, 471
LmnI	GCTCC	4 cut(s)	647, 835, 1648, 1725
Lsp1109I	GCAGC	9 cut(s)	649, 652, 798, 859, 871, 1310, 1483, 1510, 1532
LweI	GCATC	2 cut(s)	428, 1000
MaeI	CTAG	3 cut(s)	1461, 1500, 1635
MaeII	ACGT	4 cut(s)	222, 655, 1182, 1225
MaeIII	GTNAC	6 cut(s)	64, 506, 997, 1077, 1689, 1890
MflI	RGATCY	3 cut(s)	735, 1021, 1083
MhlI	GDGCHC	1 cut(s)	652
MlyI	GAGTC	4 cut(s)	162, 625, 1170, 1629
MmeI	TCCRAC	2 cut(s)	336, 675
Mph1103I	ATGCAT	1 cut(s)	1248
MroXI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
MseI	TTAA	3 cut(s)	566, 1437, 1773
MslI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
MspA1I	CMGCKG	1 cut(s)	1523
MspI	CCGG	5 cut(s)	28, 39, 58, 263, 416
MspR9I	CCNGG	3 cut(s)	339, 495, 1572
Mva1269I	GAATGC	1 cut(s)	211
MvaI	CCWGG	3 cut(s)	339, 495, 1572
MvnI	CGCG	3 cut(s)	97, 432, 473
MwoI	GCNNNNNNNGC	8 cut(s)	239, 371, 490, 881, 890, 1109, 1496, 1517
NdeI	CATATG	1 cut(s)	1595
NlaIII	CATG	9 cut(s)	154, 439, 506, 584, 755, 964, 1103, 1246, 1889
NlaIV	GGNNCC	1 cut(s)	483
NmeAIII	GCCGAG	1 cut(s)	62
NmuCI	GTSAC	3 cut(s)	997, 1077, 1890
NruI	TCGCGA	1 cut(s)	432
NsiI	ATGCAT	1 cut(s)	1248
NspI	RCATGY	1 cut(s)	1246
NspV	TTCGAA	1 cut(s)	1107
PaeI	GCATGC	1 cut(s)	1246
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	1 cut(s)	211
PdmI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
PfeI	GAWTC	4 cut(s)	112, 175, 295, 1143
PflMI	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
PleI	GAGTC	4 cut(s)	162, 624, 1169, 1628
PpsI	GAGTC	4 cut(s)	162, 624, 1169, 1628
PsiI	TTATAA	1 cut(s)	1713
Psp6I	CCWGG	3 cut(s)	337, 493, 1570
PspFI	CCCAGC	1 cut(s)	826
PspGI	CCWGG	3 cut(s)	337, 493, 1570
PspN4I	GGNNCC	1 cut(s)	483
PspPI	GGNCC	3 cut(s)	156, 254, 410
PstI	CTGCAG	2 cut(s)	146, 1300
PstNI	CAGNNNCTG	1 cut(s)	1157
PsuI	RGATCY	3 cut(s)	735, 1021, 1083
PvuII	CAGCTG	1 cut(s)	1523
RruI	TCGCGA	1 cut(s)	432
RsaI	GTAC	2 cut(s)	316, 1134
RsaNI	GTAC	2 cut(s)	315, 1133
RseI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
Rsr2I	CGGWCCG	1 cut(s)	156
RsrII	CGGWCCG	1 cut(s)	156
SalI	GTCGAC	1 cut(s)	1454
SaqAI	TTAA	3 cut(s)	566, 1437, 1773
Sau96I	GGNCC	3 cut(s)	156, 254, 410
SbfI	CCTGCAGG	1 cut(s)	146
SchI	GAGTC	4 cut(s)	162, 625, 1170, 1629
ScrFI	CCNGG	3 cut(s)	339, 495, 1572
SdaI	CCTGCAGG	1 cut(s)	146
SduI	GDGCHC	1 cut(s)	652
SfaNI	GCATC	2 cut(s)	428, 1000
SfcI	CTRYAG	2 cut(s)	142, 1296
SfiI	GGCCNNNNNGGCC	1 cut(s)	239
SfuI	TTCGAA	1 cut(s)	1107
SinI	GGWCC	2 cut(s)	156, 254
SmiMI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
SmlI	CTYRAG	1 cut(s)	1652
SmoI	CTYRAG	1 cut(s)	1652
SphI	GCATGC	1 cut(s)	1246
Sse8387I	CCTGCAGG	1 cut(s)	146
SsiI	CCGC	7 cut(s)	25, 97, 154, 159, 306, 473, 485
SspI	AATATT	1 cut(s)	690
SspMI	CTAG	3 cut(s)	1461, 1500, 1635
StyD4I	CCNGG	3 cut(s)	337, 493, 1570
StyI	CCWWGG	3 cut(s)	716, 888, 1854
TaaI	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
TaiI	ACGT	4 cut(s)	225, 658, 1185, 1228
TaqI	TCGA	9 cut(s)	293, 300, 633, 853, 976, 1107, 1455, 1945, 1951
TauI	GCSGC	3 cut(s)	308, 476, 487
TfiI	GAWTC	4 cut(s)	112, 175, 295, 1143
Tru1I	TTAA	3 cut(s)	566, 1437, 1773
Tru9I	TTAA	3 cut(s)	566, 1437, 1773
TscAI	CASTG	5 cut(s)	922, 1240, 1300, 1476, 1684
TseFI	GTSAC	3 cut(s)	997, 1077, 1890
TseI	GCWGC	9 cut(s)	662, 665, 786, 872, 884, 1298, 1496, 1520, 1523
Tsp45I	GTSAC	3 cut(s)	997, 1077, 1890
TspDTI	ATGAA	9 cut(s)	545, 639, 789, 949, 983, 1157, 1566, 1680, 1865
TspGWI	ACGGA	2 cut(s)	1068, 1844
TspRI	CASTG	5 cut(s)	922, 1240, 1300, 1476, 1684
Van91I	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
VpaK11BI	GGWCC	2 cut(s)	156, 254
XapI	RAATTY	3 cut(s)	626, 1038, 1553
XceI	RCATGY	1 cut(s)	1246
XcmI	CCANNNNNNNNNTGG	2 cut(s)	341, 433
XmiI	GTMKAC	1 cut(s)	1455
XmnI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
XspI	CTAG	3 cut(s)	1461, 1500, 1635
Zsp2I	ATGCAT	1 cut(s)	1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.