pycom12561g00260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012561
OG0016253
Physical Location & Seq
Forward (+)
382404 .. 383489
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGGAAATGGAAATCCCAGATGATTTCTGCACCTATGTTAGTGCACCCGCTAGTCCTGGTAGATATTGCAGCTCTGCCAATAACAACAATGTTTGCTTCTTTAGTGTCCCAACTAGTCCTAGGAAAAGGACCATTTCATCAACTTCTGATGACTATCCAACACCACCCATGACACCATATCACGATGCCAATTCCGACCTCAACGACTTTGAATTCGACACCAGTCACAGTTTCAATCTTGGTTCGGTTGGTAATGTGACTAGCCAGAAGGTGAAAGACTTGCTTCTGCACAAGCAGCACGAACAAATGCAAACCATGGCATTTGCTGACGACCTTTTCTGCGGCGGAAAAGTTATCCCGCTTGTACCTCCACTCAAACTTCCACCCCGTCTTGGTCAACAAAGAAATACGAACCGAAGCCCAACCCCATCTACCCCAGGGTCACCGAGTTTTGGGATCAAAATGCCGTTTTCGCATAGGACGTTGTGGAATGATGATTTTGATCCATTCATGGTGGCTCTGCAGCATGTCACGAAGGAGGAAAAGAGAGGAAAAGCAAAGACACAGGCTCGTGACATGTCCGACCTGAAAGACCAACAGAATAAACAAACTGGCCTGCCAACACCAATTTCCTCACCTTTTGTGAACTCCCCAAGTCTGCTACAAAGTCCAAAAAGGCTGGCTCTGCCCAGAGGACTGGAGTTTGCTAGACAAGTTAGACTAATCCGAATGGATCATTCCGAGAGATGCAGTGACCCCAAATTGGAGACGAGGACGCCTAAGAATAACATTGCTCTAGAAAGTGGGGGTCCATGTACAAAGCAAACCAAGCGGCAGAAAATTGGAAGGCTTTTATTGAGTGTGTCATTTGGGAAGGCAGATGATGAAGACAAAAAGGGAAAAGATCAAACTTCCGAAATATTGAAAAATCCAACATTTCTAAGGAGACTGAGCTTCAAGTCAGGAAGATTAACCAGCTGCAAAGCCGAGAAGAGGATATCTAATCAACTCACCAAGACGCCGCTAGTACAATTCAGGCCGAGGCTCTTACTCTGTATGGGTTATAAGGCAAGGTATGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

362

Amino Acids

40.62

Weight (kDa)

9.61

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06740
malus_domestica MD00G1045800.v1.1 MD03G1164300.v1.1
prunus_persica Prupe.4G260600_v2.0.a1
pyrus_communis pycom12561g00260
rosa_chinensis RchiOBHm_Chr6g0258931
rosa_laevigata RLG00000014570
rosa_multiflora Rmu_sc0004347.1_g000009
rosa_roxburghii Rroxscaffold_7G00206180
rosa_rugosa Rorug05G0584500
rosa_samantha Rh6AG100900 Rh6BG092800 Rh6CG090100 Rh6DG084300
rosa_wichuraiana Rw6G008760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AciI CCGC 6 cut(s) 48, 342, 345, 359, 832, 1022
AclWI GGATC 3 cut(s) 464, 497, 741
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 776, 1019
AfaI GTAC 3 cut(s) 366, 817, 1029
AfiI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 993
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 4 cut(s) 212, 235, 925, 958
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 2 cut(s) 55, 436
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 3 cut(s) 72, 954, 978
AluI AGCT 3 cut(s) 72, 954, 978
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 761, 940
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 3 cut(s) 464, 497, 741
AoxI GGCC 2 cut(s) 613, 1037
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 4 cut(s) 69, 295, 523, 978
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AspA2I CCTAGG 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 129, 809
AsuHPI GGTGA 4 cut(s) 283, 435, 627, 1003
AvaII GGWCC 2 cut(s) 129, 809
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 46
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 4 cut(s) 81, 307, 535, 965
BccI CCATC 1 cut(s) 436
BceAI ACGGC 1 cut(s) 451
BciT130I CCWGG 2 cut(s) 57, 438
BcoDI GTCTC 2 cut(s) 761, 940
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 7 cut(s) 51, 114, 120, 261, 708, 797, 1025
BfmI CTRYAG 1 cut(s) 521
BisI GCNGC 7 cut(s) 70, 296, 343, 524, 833, 979, 1022
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 7 cut(s) 71, 297, 344, 525, 834, 980, 1023
Bme1390I CCNGG 2 cut(s) 57, 438
Bme18I GGWCC 2 cut(s) 129, 809
BmgT120I GGNCC 2 cut(s) 129, 809
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 2 cut(s) 57, 438
BmsI GCATC 2 cut(s) 175, 737
BoxI GACNNNNGTC 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 894
BpmI CTGGAG 1 cut(s) 719
BsaBI GATNNNNATC 2 cut(s) 153, 501
BsaHI GRCGYC 2 cut(s) 776, 1019
BsaJI CCNNGG 5 cut(s) 119, 315, 436, 437, 1040
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 452, 763, 993
Bse1I ACTGG 3 cut(s) 222, 616, 702
Bse3DI GCAATG 1 cut(s) 789
Bse8I GATNNNNATC 2 cut(s) 153, 501
BseBI CCWGG 2 cut(s) 57, 438
BseDI CCNNGG 5 cut(s) 119, 315, 436, 437, 1040
BseJI GATNNNNATC 2 cut(s) 153, 501
BseLI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 993
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 1 cut(s) 941
BseNI ACTGG 3 cut(s) 222, 616, 702
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 4 cut(s) 81, 307, 535, 965
BsgI GTGCAG 2 cut(s) 13, 272
BshFI GGCC 2 cut(s) 615, 1039
BsiHKAI GWGCWC 1 cut(s) 46
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 993
BsmAI GTCTC 2 cut(s) 761, 940
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 615, 1039
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 815
Bsp143I GATC 4 cut(s) 456, 502, 733, 904
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 6 cut(s) 48, 342, 345, 359, 832, 1022
BspANI GGCC 2 cut(s) 615, 1039
BspCNI CTCAG 1 cut(s) 942
BspLI GGNNCC 1 cut(s) 810
BspMAI CTGCAG 1 cut(s) 525
BspPI GGATC 3 cut(s) 464, 497, 741
BsrDI GCAATG 1 cut(s) 789
BsrGI TGTACA 1 cut(s) 815
BsrI ACTGG 3 cut(s) 222, 616, 702
BssECI CCNNGG 5 cut(s) 119, 315, 436, 437, 1040
BssMI GATC 4 cut(s) 456, 502, 733, 904
BssNI GRCGYC 2 cut(s) 776, 1019
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 2 cut(s) 119, 315
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 57, 438
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 986
BstACI GRCGYC 2 cut(s) 776, 1019
BstAUI TGTACA 1 cut(s) 815
BstC8I GCNNGC 2 cut(s) 617, 681
BstDEI CTNAG 3 cut(s) 780, 941, 950
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 441
BstKTI GATC 4 cut(s) 459, 505, 736, 907
BstMAI GTCTC 2 cut(s) 761, 940
BstMBI GATC 4 cut(s) 456, 502, 733, 904
BstMWI GCNNNNNNNGC 4 cut(s) 295, 472, 685, 829
BstNI CCWGG 2 cut(s) 57, 438
BstNSI RCATGY 2 cut(s) 530, 580
BstPAI GACNNNNGTC 1 cut(s) 222
BstPI GGTNACC 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 55, 436
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 4 cut(s) 81, 307, 535, 965
BstV2I GAAGAC 1 cut(s) 894
BstXI CCANNNNNNTGG 1 cut(s) 697
BsuRI GGCC 2 cut(s) 615, 1039
BtgI CCRYGG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 1 cut(s) 757
Cac8I GCNNGC 2 cut(s) 617, 681
Cfr13I GGNCC 2 cut(s) 129, 809
CseI GACGC 2 cut(s) 784, 1027
Csp6I GTAC 3 cut(s) 365, 816, 1028
CviAII CATG 6 cut(s) 169, 316, 511, 527, 577, 813
CviQI GTAC 3 cut(s) 365, 816, 1028
DdeI CTNAG 3 cut(s) 780, 941, 950
DpnI GATC 4 cut(s) 458, 504, 735, 906
DpnII GATC 4 cut(s) 456, 502, 733, 904
Eam1104I CTCTTC 1 cut(s) 986
EarI CTCTTC 1 cut(s) 986
EciI GGCGGA 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 119, 315
Eco32I GATATC 1 cut(s) 999
Eco47I GGWCC 2 cut(s) 129, 809
Eco91I GGTNACC 1 cut(s) 441
EcoO65I GGTNACC 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 55, 436
EcoRV GATATC 1 cut(s) 999
EcoT14I CCWWGG 2 cut(s) 119, 315
ErhI CCWWGG 2 cut(s) 119, 315
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 6 cut(s) 172, 319, 514, 530, 580, 816
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 92
FatI CATG 6 cut(s) 168, 315, 510, 526, 576, 812
FauI CCCGC 2 cut(s) 55, 366
Fnu4HI GCNGC 7 cut(s) 70, 296, 343, 524, 833, 979, 1022
Fsp4HI GCNGC 7 cut(s) 70, 296, 343, 524, 833, 979, 1022
FspBI CTAG 7 cut(s) 51, 114, 120, 261, 708, 797, 1025
GluI GCNGC 7 cut(s) 70, 296, 343, 524, 833, 979, 1022
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 2 cut(s) 615, 1039
HgaI GACGC 2 cut(s) 784, 1027
Hin1I GRCGYC 2 cut(s) 776, 1019
Hin1II CATG 6 cut(s) 172, 319, 514, 530, 580, 816
HincII GTYRAC 1 cut(s) 398
HindII GTYRAC 1 cut(s) 398
HphI GGTGA 4 cut(s) 283, 435, 627, 1003
Hpy166II GTNNAC 3 cut(s) 44, 398, 646
Hpy188I TCNGA 6 cut(s) 148, 196, 583, 728, 742, 916
Hpy188III TCNNGA 5 cut(s) 182, 532, 572, 797, 963
Hpy8I GTNNAC 3 cut(s) 44, 398, 646
HpyAV CCTTC 4 cut(s) 262, 529, 840, 868
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 8 cut(s) 30, 44, 69, 289, 310, 523, 750, 981
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 472, 685, 829
HpyF3I CTNAG 3 cut(s) 780, 941, 950
HpySE526I ACGT 1 cut(s) 482
Hsp92I GRCGYC 2 cut(s) 776, 1019
Hsp92II CATG 6 cut(s) 172, 319, 514, 530, 580, 816
Kzo9I GATC 4 cut(s) 456, 502, 733, 904
Lsp1109I GCAGC 4 cut(s) 81, 307, 535, 965
LweI GCATC 2 cut(s) 175, 737
MaeI CTAG 7 cut(s) 51, 114, 120, 261, 708, 797, 1025
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 6 cut(s) 224, 256, 441, 529, 572, 752
MalI GATC 4 cut(s) 458, 504, 735, 906
MboI GATC 4 cut(s) 456, 502, 733, 904
MboII GAAGA 3 cut(s) 899, 978, 1003
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 6 cut(s) 190, 212, 627, 761, 840, 1031
MmeI TCCRAC 4 cut(s) 182, 219, 606, 956
MnlI CCTC 9 cut(s) 209, 378, 532, 542, 643, 686, 765, 987, 1035
MseI TTAA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 978
MspR9I CCNGG 2 cut(s) 57, 438
MvaI CCWGG 2 cut(s) 57, 438
MwoI GCNNNNNNNGC 4 cut(s) 295, 472, 685, 829
NcoI CCATGG 1 cut(s) 315
NdeII GATC 4 cut(s) 456, 502, 733, 904
NlaIII CATG 6 cut(s) 172, 319, 514, 530, 580, 816
NlaIV GGNNCC 1 cut(s) 810
NmeAIII GCCGAG 2 cut(s) 1012, 1065
NmuCI GTSAC 6 cut(s) 224, 256, 441, 529, 572, 752
NspI RCATGY 2 cut(s) 530, 580
PasI CCCWGGG 1 cut(s) 437
PciI ACATGT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PkrI GCNGC 7 cut(s) 71, 297, 344, 525, 834, 980, 1023
PscI ACATGT 1 cut(s) 576
PshAI GACNNNNGTC 1 cut(s) 222
PsiI TTATAA 1 cut(s) 1065
Psp6I CCWGG 2 cut(s) 55, 436
PspEI GGTNACC 1 cut(s) 441
PspGI CCWGG 2 cut(s) 55, 436
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 2 cut(s) 129, 809
PstI CTGCAG 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 978
RsaI GTAC 3 cut(s) 366, 817, 1029
RsaNI GTAC 3 cut(s) 365, 816, 1028
SaqAI TTAA 1 cut(s) 971
SatI GCNGC 7 cut(s) 70, 296, 343, 524, 833, 979, 1022
Sau3AI GATC 4 cut(s) 456, 502, 733, 904
Sau96I GGNCC 2 cut(s) 129, 809
ScrFI CCNGG 2 cut(s) 57, 438
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 2 cut(s) 175, 737
SfcI CTRYAG 1 cut(s) 521
SinI GGWCC 2 cut(s) 129, 809
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 6 cut(s) 190, 212, 627, 761, 840, 1031
SsiI CCGC 6 cut(s) 48, 342, 345, 359, 832, 1022
SspI AATATT 1 cut(s) 921
SspMI CTAG 7 cut(s) 51, 114, 120, 261, 708, 797, 1025
StyD4I CCNGG 2 cut(s) 55, 436
StyI CCWWGG 2 cut(s) 119, 315
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 1 cut(s) 485
TaqI TCGA 1 cut(s) 216
TasI AATT 6 cut(s) 190, 212, 627, 761, 840, 1031
TatI WGTACW 2 cut(s) 815, 1027
TauI GCSGC 3 cut(s) 345, 835, 1024
Tru1I TTAA 1 cut(s) 971
Tru9I TTAA 1 cut(s) 971
TscAI CASTG 1 cut(s) 757
TseFI GTSAC 6 cut(s) 224, 256, 441, 529, 572, 752
TseI GCWGC 4 cut(s) 69, 295, 523, 978
Tsp45I GTSAC 6 cut(s) 224, 256, 441, 529, 572, 752
TspDTI ATGAA 3 cut(s) 126, 499, 900
TspRI CASTG 1 cut(s) 757
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 2 cut(s) 129, 809
XapI RAATTY 1 cut(s) 212
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 2 cut(s) 530, 580
XmaJI CCTAGG 1 cut(s) 119
XspI CTAG 7 cut(s) 51, 114, 120, 261, 708, 797, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.