pycom12654g00120

Histone chaperone domain CHZ

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012654
OG0007419
Physical Location & Seq
Forward (+)
136064 .. 141785
5722 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
ATGGCGGATGACGCGCAAGATAGCGAGATCCCTCTGAAGGAGGCGCATGATATCCAGTCCCAGATTAAGACTGCTATGCGCTTACGCGTCCCTTACTTCAAAGAACAATCCGATTCTTTGACTTTTGAGGGTGTCCGAAGAGTCTTAGAGAAAGACCTGGGCTTGGAAACATTTGCGTTGGATGTGCACAAAAGATTTGTCAAGGAACATTTAGTGGAGGTCTTAGAAGGTGCTGGTGATGACAATACCTCAAGGGCATCAGGGGAGACTGATGAAAAAACTCTGGTTAAAGAAGAAGCCGTAGAATTACCAAAAGGAAAGTCAAATAAAGATGCGAAGCAAACATGTTCAGAAGATGAGGAGAAAATGGAAGACTCTCCTGTAATGGGGCTTCTGACAGGGCATAAAGCGGCCAAATCTGGAACTGAAGAAACTAAAACTACCAACCAAAAAAAGGGTCCAACTGAGAGTGTGATAAAGTCAGCCCTTATAAAAAGGGGTTCTTATATCAAAGCTAATTCTGAAAAGATTACTCTGGTTGGACTTCGCCGACTTTTGGAGGAAGATCTTCAGCTTCAGAAATATACTCTTGATTCCTTTAAGAAGTTTATAAGTCAGCAACTAGACGAGGTTCTAGAATCTTGTGAAAATTCTGAACCTGCAACTACTGCCAAGAAAAATGTTCAGAGGAGTGCTCAGAGAAAAGCATCCACTAAGGTGAGAAGCAATGAGAGTTCTGGTTCTTCAGATAATGAAAGTAGTGAGGAAGAAGATGAAGTAAAACCTAAAAGAAAAACTGCTCCAAAAGGAAAGACGCAGAAAAGTGCTCAGAGAAAAGCATCCACTAAGGTCAGAAGCGATGAGAGATCTGGTTCTTCAGATAATGAAATTAGTGAGGAAGAAGATGAAGTAAAACCTAGAAGCAAAAGTGTTCCAAAAGGAAAGATGCAGAATATGGACAGCCATAAGAAACGGAAAATAATGGCTAAGGAGACTAACGTCTCTGGAAAGAAGAGGATCAAGCCTCCTCAGAAAGACCCAGAAGAAAAAAGTGATGCAGAAGACAGTGGAAATGTCTCTGAAGATGACCATTCTCAGTCATCTGCCGAAAAACCTGTAAAGAAGAAAGAAGTTTCAACAACACCTGCATATGGGAAACGTGTGGAGCATCTACGATCAGTTATCAAAGCATGTGGAATGAGCGTTGCCCCCTCAGTATACAAGAAAGTCAAGCAGGTGCCTGAGAATAAACGTGAAGCGCACCTGATAAAGGAGTTAGAGGAGATACTCGCTAGAGAAGGATTATCTGCACATCCCTCTGAGAAAGAAATCAAGGAAGTCAAAAAGAAAAAGGAAAGGGCAAAAGAGCTCGAAGGCATTGACATGAGTAACATTGTAACAAGTTCACGTAGAAGGTCCACTACTAGTTTTGTGCCTCCTCCAAAGCCCCAAATACCAGTTGAAAGTGAATCTGATGATGCTGAAAGTTCAGATGATGACAATGATGAAGATGACGACAGTGACAAGGAAGAGAATGTGGTTGAGGACGAGGACAATGGGGATAATGGTAATAGTGATAGCAACCATAGTGACAAGGATGAAGCTGATGACAGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHZ

Protein Analysis

540

Amino Acids

60.05

Weight (kDa)

5.36

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHZ PF09649 452 - 483 1.1e-09 Histone chaperone domain CHZ
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 491, 611
AarI CACCTGC 2 cut(s) 1153, 1225
Acc36I ACCTGC 3 cut(s) 667, 1153, 1225
AccB1I GGYRCC 1 cut(s) 1237
AccI GTMKAC 1 cut(s) 1218
AccII CGCG 2 cut(s) 14, 87
AciI CCGC 2 cut(s) 5, 410
AclWI GGATC 2 cut(s) 22, 1025
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 7 cut(s) 56, 447, 554, 560, 729, 861, 1101
AfiI CCNNNNNNNGG 7 cut(s) 37, 163, 386, 454, 556, 1151, 1270
AflIII ACRYGT 3 cut(s) 85, 344, 1159
AgsI TTSAA 3 cut(s) 100, 1137, 1463
AhlI ACTAGT 1 cut(s) 1424
AjnI CCWGG 1 cut(s) 156
AleI CACNNNNGTG 1 cut(s) 716
AluBI AGCT 4 cut(s) 515, 574, 1369, 1604
AluI AGCT 4 cut(s) 515, 574, 1369, 1604
Alw21I GWGCWC 4 cut(s) 189, 697, 829, 1371
Alw26I GTCTC 4 cut(s) 260, 986, 1006, 1081
Alw44I GTGCAC 1 cut(s) 185
AlwI GGATC 2 cut(s) 22, 1025
AoxI GGCC 1 cut(s) 411
ApaLI GTGCAC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 6 cut(s) 183, 215, 305, 337, 442, 474
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 4 cut(s) 16, 46, 81, 1261
AspS9I GGNCC 2 cut(s) 458, 1416
AsuHPI GGTGA 2 cut(s) 248, 730
AvaII GGWCC 2 cut(s) 458, 1416
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 1237
BanII GRGCYC 1 cut(s) 1371
BarI GAAGNNNNNNTAC 4 cut(s) 375, 407, 1405, 1437
BbsI GAAGAC 2 cut(s) 378, 1068
Bbv12I GWGCWC 4 cut(s) 189, 697, 829, 1371
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 158
BcoDI GTCTC 4 cut(s) 260, 986, 1006, 1081
BcuI ACTAGT 1 cut(s) 1424
BfaI CTAG 5 cut(s) 623, 635, 918, 1293, 1425
BfuAI ACCTGC 3 cut(s) 667, 1153, 1225
BglII AGATCT 2 cut(s) 565, 866
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 2 cut(s) 458, 1416
BmgT120I GGNCC 2 cut(s) 458, 1416
BmiI GGNNCC 2 cut(s) 459, 1239
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 8 cut(s) 266, 322, 716, 848, 936, 1045, 1177, 1468
BoxI GACNNNNGTC 1 cut(s) 998
BpiI GAAGAC 2 cut(s) 378, 1068
BplI GAGNNNNNCTC 2 cut(s) 679, 711
Bpu10I CCTNAGC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 1409
BsaJI CCNNGG 1 cut(s) 157
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 163, 386, 454, 556, 1151, 1270
Bse1I ACTGG 2 cut(s) 55, 1457
Bse3DI GCAATG 1 cut(s) 733
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 6 cut(s) 13, 187, 707, 839, 1312, 1603
BseLI CCNNNNNNNGG 7 cut(s) 37, 163, 386, 454, 556, 1151, 1270
BseMI GCAATG 1 cut(s) 733
BseMII CTCAG 8 cut(s) 456, 710, 842, 1043, 1109, 1227, 1233, 1311
BseNI ACTGG 2 cut(s) 55, 1457
BseRI GAGGAG 5 cut(s) 374, 703, 1017, 1295, 1428
BseSI GKGCMC 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 2 cut(s) 14, 87
BshFI GGCC 1 cut(s) 413
BshNI GGYRCC 1 cut(s) 1237
BsiHKAI GWGCWC 4 cut(s) 189, 697, 829, 1371
BslFI GGGAC 2 cut(s) 43, 74
BslI CCNNNNNNNGG 7 cut(s) 37, 163, 386, 454, 556, 1151, 1270
BsmAI GTCTC 4 cut(s) 260, 986, 1006, 1081
BsmBI CGTCTC 1 cut(s) 1006
BsmFI GGGAC 2 cut(s) 43, 74
BsnI GGCC 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 189, 697, 829, 1371
Bsp143I GATC 5 cut(s) 27, 565, 866, 1017, 1175
BspACI CCGC 2 cut(s) 5, 410
BspANI GGCC 1 cut(s) 413
BspCNI CTCAG 8 cut(s) 457, 709, 841, 1042, 1108, 1226, 1234, 1312
BspFNI CGCG 2 cut(s) 14, 87
BspLI GGNNCC 2 cut(s) 459, 1239
BspMI ACCTGC 3 cut(s) 667, 1153, 1225
BspPI GGATC 2 cut(s) 22, 1025
BspT107I GGYRCC 1 cut(s) 1237
BsrDI GCAATG 1 cut(s) 733
BsrI ACTGG 2 cut(s) 55, 1457
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 5 cut(s) 27, 565, 866, 1017, 1175
BssNAI GTATAC 1 cut(s) 1219
Bst1107I GTATAC 1 cut(s) 1219
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 1067, 1520
Bst6I CTCTTC 3 cut(s) 133, 1007, 1524
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 1409
BstENI CCTNNNNNAGG 1 cut(s) 1268
BstF5I GGATG 6 cut(s) 13, 187, 707, 839, 1312, 1603
BstFNI CGCG 2 cut(s) 14, 87
BstHHI GCGC 4 cut(s) 16, 46, 81, 1261
BstKTI GATC 5 cut(s) 30, 568, 869, 1020, 1178
BstMAI GTCTC 4 cut(s) 260, 986, 1006, 1081
BstMBI GATC 5 cut(s) 27, 565, 866, 1017, 1175
BstMWI GCNNNNNNNGC 2 cut(s) 11, 668
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 2 cut(s) 348, 1194
BstPAI GACNNNNGTC 1 cut(s) 998
BstSCI CCNGG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 189
BstUI CGCG 2 cut(s) 14, 87
BstV2I GAAGAC 2 cut(s) 378, 1068
BstX2I RGATCY 3 cut(s) 27, 565, 866
BstYI RGATCY 3 cut(s) 27, 565, 866
BstZ17I GTATAC 1 cut(s) 1219
BsuRI GGCC 1 cut(s) 413
BtgZI GCGATG 1 cut(s) 873
BtsCI GGATG 6 cut(s) 13, 187, 707, 839, 1312, 1603
BtsIMutI CAGTG 2 cut(s) 1072, 1525
BveI ACCTGC 3 cut(s) 667, 1153, 1225
CfoI GCGC 4 cut(s) 16, 46, 81, 1261
Cfr13I GGNCC 2 cut(s) 458, 1416
CseI GACGC 3 cut(s) 20, 76, 823
CspCI CAANNNNNGTGG 2 cut(s) 1174, 1209
CviAII CATG 4 cut(s) 47, 345, 1191, 1384
DpnI GATC 5 cut(s) 29, 567, 868, 1019, 1177
DpnII GATC 5 cut(s) 27, 565, 866, 1017, 1175
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 133, 1007, 1524
EarI CTCTTC 3 cut(s) 133, 1007, 1524
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 1369
Eco24I GRGCYC 1 cut(s) 1371
Eco32I GATATC 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 458, 1416
Eco53kI GAGCTC 1 cut(s) 1369
Eco57I CTGAAG 7 cut(s) 56, 447, 554, 560, 729, 861, 1101
EcoICRI GAGCTC 1 cut(s) 1369
EcoNI CCTNNNNNAGG 1 cut(s) 1268
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 1371
Esp3I CGTCTC 1 cut(s) 1006
FaeI CATG 4 cut(s) 50, 348, 1194, 1387
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FaqI GGGAC 2 cut(s) 43, 74
FatI CATG 4 cut(s) 46, 344, 1190, 1383
FauNDI CATATG 1 cut(s) 1150
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 6 cut(s) 20, 194, 694, 826, 1299, 1610
FriOI GRGCYC 1 cut(s) 1371
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 5 cut(s) 623, 635, 918, 1293, 1425
GlaI GCGC 4 cut(s) 15, 45, 80, 1260
GluI GCNGC 1 cut(s) 411
HaeIII GGCC 1 cut(s) 413
HgaI GACGC 3 cut(s) 20, 76, 823
HhaI GCGC 4 cut(s) 16, 46, 81, 1261
Hin1II CATG 4 cut(s) 50, 348, 1194, 1387
Hin6I GCGC 4 cut(s) 14, 44, 79, 1259
HinP1I GCGC 4 cut(s) 14, 44, 79, 1259
HinfI GANTC 6 cut(s) 113, 141, 374, 593, 638, 1469
HphI GGTGA 2 cut(s) 248, 730
Hpy166II GTNNAC 4 cut(s) 187, 1219, 1406, 1419
Hpy188III TCNNGA 4 cut(s) 420, 590, 635, 1005
Hpy8I GTNNAC 4 cut(s) 187, 1219, 1406, 1419
HpyAV CCTTC 5 cut(s) 31, 221, 1292, 1367, 1407
HpyCH4III ACNGT 2 cut(s) 1067, 1520
HpyCH4IV ACGT 4 cut(s) 999, 1159, 1252, 1408
HpyCH4V TGCA 6 cut(s) 187, 662, 949, 1058, 1148, 1310
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 668
HpySE526I ACGT 4 cut(s) 999, 1159, 1252, 1408
Hsp92II CATG 4 cut(s) 50, 348, 1194, 1387
HspAI GCGC 4 cut(s) 14, 44, 79, 1259
Kzo9I GATC 5 cut(s) 27, 565, 866, 1017, 1175
LmnI GCTCC 2 cut(s) 805, 1165
LweI GCATC 8 cut(s) 266, 322, 716, 848, 936, 1045, 1177, 1468
MaeI CTAG 5 cut(s) 623, 635, 918, 1293, 1425
MaeII ACGT 4 cut(s) 999, 1159, 1252, 1408
MaeIII GTNAC 4 cut(s) 1388, 1396, 1520, 1589
MalI GATC 5 cut(s) 29, 567, 868, 1019, 1177
MboI GATC 5 cut(s) 27, 565, 866, 1017, 1175
MflI RGATCY 3 cut(s) 27, 565, 866
MhlI GDGCHC 4 cut(s) 189, 697, 829, 1371
MluCI AATT 4 cut(s) 305, 517, 649, 888
MluI ACGCGT 1 cut(s) 85
MlyI GAGTC 2 cut(s) 150, 368
MmeI TCCRAC 3 cut(s) 159, 485, 520
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 4 cut(s) 66, 288, 600, 1618
MslI CAYNNNNRTG 2 cut(s) 716, 1382
MspR9I CCNGG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 2 cut(s) 14, 87
MwoI GCNNNNNNNGC 2 cut(s) 11, 668
NdeI CATATG 1 cut(s) 1150
NdeII GATC 5 cut(s) 27, 565, 866, 1017, 1175
NlaIII CATG 4 cut(s) 50, 348, 1194, 1387
NlaIV GGNNCC 2 cut(s) 459, 1239
NmuCI GTSAC 2 cut(s) 1520, 1589
NspI RCATGY 2 cut(s) 348, 1194
OliI CACNNNNGTG 1 cut(s) 716
PaqCI CACCTGC 2 cut(s) 1153, 1225
PciI ACATGT 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 4 cut(s) 113, 593, 638, 1469
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 2 cut(s) 149, 368
PpsI GAGTC 2 cut(s) 149, 368
Ppu21I YACGTR 1 cut(s) 1409
PscI ACATGT 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 998
PsiI TTATAA 2 cut(s) 491, 611
Psp124BI GAGCTC 1 cut(s) 1371
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 459, 1239
PspPI GGNCC 2 cut(s) 458, 1416
PsuI RGATCY 3 cut(s) 27, 565, 866
RseI CAYNNNNRTG 2 cut(s) 716, 1382
SacI GAGCTC 1 cut(s) 1371
SaqAI TTAA 4 cut(s) 66, 288, 600, 1618
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 5 cut(s) 27, 565, 866, 1017, 1175
Sau96I GGNCC 2 cut(s) 458, 1416
SchI GAGTC 2 cut(s) 150, 368
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 4 cut(s) 189, 697, 829, 1371
SfaNI GCATC 8 cut(s) 266, 322, 716, 848, 936, 1045, 1177, 1468
SinI GGWCC 2 cut(s) 458, 1416
SmiMI CAYNNNNRTG 2 cut(s) 716, 1382
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SpeI ACTAGT 1 cut(s) 1424
Sse9I AATT 4 cut(s) 305, 517, 649, 888
SsiI CCGC 2 cut(s) 5, 410
SspMI CTAG 5 cut(s) 623, 635, 918, 1293, 1425
SstI GAGCTC 1 cut(s) 1371
StyD4I CCNGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 1067, 1520
TaiI ACGT 4 cut(s) 1002, 1162, 1255, 1411
TaqI TCGA 1 cut(s) 1371
TasI AATT 4 cut(s) 305, 517, 649, 888
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 4 cut(s) 113, 593, 638, 1469
Tru1I TTAA 4 cut(s) 66, 288, 600, 1618
Tru9I TTAA 4 cut(s) 66, 288, 600, 1618
TscAI CASTG 2 cut(s) 1072, 1525
TseFI GTSAC 2 cut(s) 1520, 1589
Tsp45I GTSAC 2 cut(s) 1520, 1589
TspDTI ATGAA 7 cut(s) 288, 768, 789, 900, 921, 1521, 1614
TspGWI ACGGA 1 cut(s) 988
TspRI CASTG 2 cut(s) 1072, 1525
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 458, 1416
XagI CCTNNNNNAGG 1 cut(s) 1268
XapI RAATTY 1 cut(s) 649
XbaI TCTAGA 1 cut(s) 634
XceI RCATGY 2 cut(s) 348, 1194
XmiI GTMKAC 1 cut(s) 1218
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 5 cut(s) 623, 635, 918, 1293, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.